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Small extracellular vesicles derived from cerebral endothelial cells with elevated microRNA 27a promote ischemic stroke recovery

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Neural Regeneration Research 2025年第1期20卷 224-233页

作者： Yi Zhang Zhongwu Liu Michael Chopp Michael Millman Yanfeng Li Pasquale Cepparulo Amy Kemper Chao Li Li Zhang Zheng Gang Zhang department of Neurology Henry Ford HospitalDetroitMIUSA department of Physics Oakland UniversityRochesterMIUSA department of Pathology Henry Ford HospitalDetroitMIUSA Division of Pharmacology Department of NeuroscienceReproductive and Dentistry SciencesSchool of MedicineUniversity of Naples“Federico Ⅱ”NaplesItaly

Axonal remodeling is a critical aspect of ischemic brain repair processes and contributes to spontaneous functional *** previous in vitro study demonstrated that exosomes/small extracellular vesicles(sEVs)isolated from cerebral endothelial cells(CEC-sEVs)of ischemic brain promote axonal growth of embryonic cortical neurons and that microRNA 27a(miR-27a)is an elevated miRNA in ischemic *** the present study,we investigated whether normal CEC-sEVs engineered to enrich their levels of miR-27a(27a-sEVs)further enhance axonal growth and improve neurological outcomes after ischemic stroke when compared with treatment with non-engineered CEC-sEVs.27a-sEVs were isolated from the conditioned medium of healthy mouse CECs transfected with a lentiviral miR-27a expression *** EVs isolated from CECs transfected with a scramble vector(Scra-sEVs)were used as a *** male mice were subjected to permanent middle cerebral artery occlusion and then were randomly treated with 27a-sEVs or *** array of behavior assays was used to measure neurological *** with treatment of ischemic stroke with Scra-sEVs,treatment with 27a-sEVs significantly augmented axons and spines in the peri-infarct zone and in the corticospinal tract of the spinal grey matter of the denervated side,and significantly improved neurological *** vitro studies demonstrated that CEC-sEVs carrying reduced miR-27a abolished 27a-sEV-augmented axonal *** analysis revealed that 27a-sEVs systemically administered preferentially localized to the pre-synaptic active zone,while quantitative reverse transcription-polymerase chain reaction and Western Blot analysis showed elevated miR-27a,and reduced axonal inhibitory proteins Semaphorin 6A and Ras Homolog Family Member A in the peri-infarct *** of the Clathrin-dependent endocytosis pathway substantially reduced neuronal internalization of *** data provide evidence that 27a-sEVs have

关键词： axonal remodeling cerebral endothelial cells exosomes miR-27a mitochondria Semaphorin 6A small extracellular vesicles stroke

Relationship of Periodontal Status and Dental Caries Status with Oral Health Knowledge, Attitude and Behavior among Professional Students in India

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International Journal of Oral Science 2009年第4期1卷 196-206页

作者： Archana J. Sharda Srinath Shetty department of preventive and community dentistry Pacific Dental College & HospitalDebari Rajasthan India

Aim To find the relationship of periodontal status and dental caries status with oral health knowledge, attitude, behavior, among professional students in India. Methodology In a cross sectional study, a total of 825 students （males： 577, females： 248） fi＇om six professions were surveyed using a self administered structured questionnaire including 41 multiple choice questions and the WHO Oral Health Assessment Form （1997）. The data was analyzed using the SPSS version 13.0 to perform the Student＇s t-test, ANOVA test, Scheffe＇s test and Chi-square test, linear regression analysis. Results The mean percentage scores of the students for knowledge were 53.25 ±15.05; for attitude 74.97±20.48; and for behavior 59.09 ±18.77. The percentage of students with calculus score was found to be significantly high （43.8%）. The percentage of professional students with DMFT〉4 was 14.1% and the percentage of students with decayed teeth was 46.2%. The regression analysis showed that the oral health behavior of the students was dependent on the attitude （P〈0.001）, but showed no significant linear relation with the knowledge. Also, that the mean DMFT score was dependent on the oral health behavior （P〈0.05）, but showed no significant relationship with the knowledge and attitude of the students. The periodontal status was independent on the knowledge, but showed a significant relationship with attitude and behavior of the students. Conclusion A positive attitude and adherence to good oral hygiene behaviors is associated with better oral health.

关键词： oral health knowledge attitude oral healthbehavior professional students

Root canal morphology and configuration of 123 maxillary second molars by means of micro-CT

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International Journal of Oral Science 2017年第1期9卷 33-37页

作者： Thomas Gerhard Wolf Frank Paqué Anja-Christin Woop Brita Willershausen Benjamín Briseno-Marroquín department of Operative dentistry Johannes Gutenberg University Medical CenterMainzGermany Division of Endodontology Department of Preventive DentistryPeriodontology and CariologyUniversity of ZurichZurichSwitzerland

The aim of this study was to investigate the root canal configuration, accessory canals and number of main foramina of 123 maxillary second molars by means of micro-computed tomography. The teeth were scanned and reproduced with 3D software imaging. The root canal configuration and number of main foramina were evaluated by means of a four-digit system. The morphological complexity of human maxillary second molars is depicted by the number of accessory and connecting canals. The most frequently observed root canal configurations in the mesiobuccal root were 2-2-2/2 （19.5%）, 2-2-1/1 （14.6%） and 2-1-1/1 （13.0%）. A 1-1-1/1 configuration was observed in 93.5% and in 96.7% in the distobuccal and palatal roots, respectively. The MB1 mot canal had one accessory canal （18.7%）, and 8.9% of the MB2 root canal had one or two accessory canals. The distobuccal （11.3%） and palatal （14.6%） root canals had at least one accessory canal, and connecting canals were observed in 16.3% of mesiobuccal roots. The MB1, MB2, distobuccal and palatal root canals had one main foramen in 99.2%, 43.1%, 98.4% and 99.2% of samples, respectively. In the mesiobuccal root, one accessory foramen was detected in 14.6%, two were detected in 7.3%, and three were detected in 5.7%. The distobuccal root showed one or two accessory foramina in 9.1% of samples. The root canal configuration of maxillary second molars is quite heterogeneous; the mesiobuccal root has predominantly two root canal entrances （58.4%, 1 in 41.1%） with one main foramen （54.4%）. Two main foramina were observed in 43.0%. Morphological variations, connecting and accessory canals were observed in all apical thirds.

关键词： accessory and connecting canals apical foramina maxillary second molar micro-CT morphology root canal configuration

Three-dimensional analysis of the physiological foramen geometry of maxillary and mandibular molars by means of micro-CT

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International Journal of Oral Science 2017年第3期9卷 151-157页

作者： Thomas Gerhard Wolf Frank Paqué Michael Sven Patyna Brita Willershausen Benjamín Briseno-Marroquín department of Operative dentistry University Medical Center of the Johannes Gutenberg University Mainz department of Endodontology Division of Preventive DentistryPeriodontologyand CariologyCenter of Dental MedicineUniversity of Zürich

The aim of this study was to investigate the physiological foramen diameter, shape and distance between physiological and anatomical apex of maxillary and mandibular first and second molars. Accurate knowledge of the physiological foramina morphology; thus, inherent mechanical shaping technical hindrances, is decisive when taking the corresponding root canal final preparation decision. The morphological dimensions of a total of 1 727 physiological foramina were investigated by means of micro-computed tomography. Mean narrow and wide （to a high number, oval） diameters of the physiological foramen were 0.24, 0.22 and 0.33 mm and 0.33, 0.31 and 0.42 mm in mesiobuccal （MB）, distobuccal （DB） and palatal （P） roots in maxillary first molars; 0.24, 0.22 and 0.33 mm and 0.41, 0.33 and 0.44 in MB, DB, and P roots in maxillary second molars. Mandibular first molars showed mean narrow and wide diameters of 0.24 and 0.30 mm and of 0.39 and 0.46 mm in mesial （M） and distal （D） roots; second mandibular molars showed 0.25 and 0.31 mm and 0.47 mm in M and D roots. The mean distance between the physiological foramina and anatomical apex was 0.82, 0.81 and 1.02 mm and 0.54, 0.43 and 0.63 mm in MB, DB and P roots of the maxillary first and second molars, respectively. A mean distance of 0.95 mm （M） and 1.05 mm （D） in the first and 0.78 mm （M） and 0.81 mm （D） in the second mandibular molars was observed. Based on the results obtained, assumable recommendations for final preparation size of the physiological foramen were calculated. However, when taking into consideration, the resulting standard deviations of marginal errors must be cautiously considered when taking a final decision in clinical endodontic treatment.

关键词： apical constriction final apical file maxillary and mandibular molars morphology micro-computed tomography physiological foramen

Evaluation of periodontal healing in class II furcation defects following guided tissue regeneration with two different types of poly lactic acid membranes

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Chinese Medical Journal 2004年第2期17卷 270-274页

作者： Deniz Cetiner Berrinnsal Ates Parlar ElifGültekin BülentKurtis department of periodontology Faculty of dentistry Gazi University Ankara TurkeyDepartment of Periodontology Faculty of Dentistry Gazi University Ankara TurkeyDepartment of Periodontology Faculty of Dentistry Gazi University Ankara TurkeyDepartment of Pathology Faculty of Dentistry Gazi University Ankara TurkeyDepartment of Periodontology Faculty of Dentistry Gazi University Ankara Turkey

Background Guided tissue regeneration procedures provide predictable reconstruction of periodontal tissues in the treatment of furcation involvements in animals and huamns. This study was to compare long-term effectiveness of two different types of polylactic acid (PLA) membranes on periodontal regeneration in surgically created class II furcation defects in dogs.Methods Full thickness mucoperiosteal flap was raised on the buccal aspects of the experimental teeth and class II furcation defects having 5 mm vertical dimensions were created on mandibular premolar III and IV on each quadrant. The exposed root surfaces were thoroughly planed and PLA membranes were placed over the experimental defects on both sites. One site received liquid polymer membrane (LPM), and resorbable periodontal mesh (RPM) membranes were applied to the other site. The animals were sacrificed at 7 months after surgery and the specimens were processed for histological evaluation.Results The average length of new attachment formed on the treated roots in both groups ranged from 3. 02 mm to 4. 5 mm. Complete bone filling was observed at the furcation sites. No statistically significant differences were found between two membranes in any of the parameters (P>0. 05).Conclusion This study demonstrates favorable regenerative outcomes by the use of two different types of PLA membranes that could be used as alternatives for guided tissue regeneration (GTR).

关键词： guided tissue regeneration polyfactic acid membranes furcation/surgery experimental studies

Effect of gamification applications on success of dentistry students

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World Journal of Methodology 2025年第1期15卷 80-87页

作者： Hatice Ahsen Deniz Elif Polat Balkan Burak İncebeyaz Kıvanç Kamburoğlu department of Dentomaxillofacial Radiology Ankara University Faculty of DentistryAnkara 06500Türkiye

BACKGROUND Gamification,the use of game materials in education,has been used in several scientific fields,and studies have demonstrated its effectiveness in student *** To evaluate whether the success of 4th-and 5th-grade dentistry students increases when using the gamification applications Kahoot!and *** An experimental design was used to measure the effect of gamification applications on students who were taught using the traditional *** this purpose,Mentimeter and Kahoot!applications were used in this experimental *** groups of 4th-and 5th-grade students were formed:Mentimeter,Kahoot!,and control *** effect of gamification applications on achievement was evaluated by administering a post-test to these groups after the instructional ***-way analysis of variance and t-tests were used for statistical *** A total of 35.5%of the students were in the Mentimeter group,29.8%in the Kahoot!group,and 34.6%in the control *** the total number of students,54.8%were female and 45.2%were male,while 52.2%were 5th-grade students and 47.8%were 4th-grade *** overall grade point average was found to be 66.65(range,24-100).There was a statistically significant difference in average success scores between students of different groups(P=0.003<0.05)and grades(P=0.036<0.05).The average success score was the highest in the Mentimeter group(71.64)and the lowest in the Kahoot!group(62.15).In the control group,this score was *** average success score was higher for students in the 5thgrade(68.16)and for female students(68.84).CONCLUSION Gamification has a great potential in the education of dentomaxillofacial radiology *** outperformed both the Kahoot!and the traditional method in terms of average student success rates.

关键词： Gamification Mentimeter Kahoot! Education dentistry

The emerging role of mesenchymal stem cell-derived extracellular vesicles to ameliorate hippocampal NLRP3 inflammation induced by binge-like ethanol treatment in adolescence

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Neural Regeneration Research 2025年第4期20卷 1153-1163页

作者： Susana Mellado María JoséMorillo-Bargues Carla Perpiñá-Clérigues Francisco García-García Victoria Moreno-Manzano Consuelo Guerri María Pascual department of Physiology School of Medicine and DentistryUniversity of ValenciaValenciaSpain Bioinformatics and Biostatistics Unit Príncipe Felipe Research CenterValenciaSpain Neuronal and Tissue Regeneration Laboratory Príncipe Felipe Research CenterValenciaSpain

Our previous studies have reported that activation of the NLRP3(NOD-,LRR-and pyrin domain-containing protein 3)-inflammasome complex in ethanol-treated astrocytes and chronic alcohol-fed mice could be associated with neuroinflammation and brain *** stem cell-derived extracellular vesicles(MSC-EVs)have been shown to restore the neuroinflammatory response,along with myelin and synaptic structural alterations in the prefrontal cortex,and alleviate cognitive and memory dysfunctions induced by binge-like ethanol treatment in adolescent *** the therapeutic role of the molecules contained in mesenchymal stem cell-derived extracellular vesicles,the present study analyzed whether the administration of mesenchymal stem cell-derived extracellular vesicles isolated from adipose tissue,which inhibited the activation of the NLRP3 inflammasome,was capable of reducing hippocampal neuroinflammation in adolescent mice treated with binge *** demonstrated that the administration of mesenchymal stem cell-derived extracellular vesicles ameliorated the activation of the hippocampal NLRP3 inflammasome complex and other NLRs inflammasomes(e.g.,pyrin domain-containing 1,caspase recruitment domain-containing 4,and absent in melanoma 2,as well as the alterations in inflammatory genes(interleukin-1β,interleukin-18,inducible nitric oxide synthase,nuclear factor-kappa B,monocyte chemoattractant protein-1,and C–X3–C motif chemokine ligand 1)and miRNAs(miR-21a-5p,miR-146a-5p,and miR-141-5p)induced by binge-like ethanol treatment in adolescent *** analysis further revealed the involvement of miR-21a-5p and miR-146a-5p with inflammatory target genes and NOD-like receptor signaling *** together,these findings provide novel evidence of the therapeutic potential of MSC-derived EVs to ameliorate the hippocampal neuroinflammatory response associated with NLRP3 inflammasome activation induced by binge drinking in adolescence.

关键词： adolescence binge-like ethanol treatment extracellular vesicles hippocampus mesenchymal stem cells neuroinflammation NOD-,LRR-and pyrin domain-containing protein 3(NLRP3)

Periodontopathogen profile of healthy and oral lichen planus patients with gingivitis or periodontitis

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International Journal of Oral Science 2013年第2期5卷 92-97页

作者： Abdullah Seckin Ertugrul Ugur Arslan Recep Dursun Sema Sezgin Hakki department of periodontology Faculty of DentistryYuzuncu Yil University department of Microbiology Selcuklu Medical FacultySelcuk University department of Dermatology Baskent Research Hospital department of periodontology Faculty of DentistrySelcuk University

Oral lichen planus （OLP） is a chronic inflammatory disease that is frequently detected in oral tissues. The aim of our study was to identify the prevalence of the detection of periodontopathogenic microorganisms （Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia and Treponema denticola in OLP patients and to compare with this prevalence of periodontopathogenic microorganisms in healthy non-OLP patients. Our study included 27 （18 chronic periodontitis （OLPP） and 9 gingivitis （OLPG）） patients diagnosed with OLP along with 26 （13 chronic periodontitis （HP） and 13 gingivitis （HG）） healthy non-OLP patients. The multiplex polymerase chain reaction （PCR）with subsequent reverse hybridization method （micro-IDent） was used for identifying periodontopathogenic microorganisms present in subgingival plaque samples. The percentages of detection for A. actinomycetemcomitans, P. gingivalis, P. intermedia, T. forsythia and T. denticola in subgingival plaque samples taken from OLP patients （OLPG and OLPP） were 18.5%, 85.1%, 81.4%, 88.8% and 74%, respectively. Meanwhile, in the non-OLP patients （HG and HP）, these values were 7.6%, 50%, 46.1%, 73% and 57.7%, respectively. Thus, comparing the non-OLP groups with the OLP groups, the periodontopathogens＇ percentages of detection in the OLP groups were higher than those in the non-OLP groups. According to our study results, OLP patients have higher levels of infection with A. actinomycetemcomitans, P. gingivalis, P. intermedia, T. forsythia and T. denticola than non-OLP patients. We argue that the high percentages in patients with OLP may help identify the importance of periodontopathoRenic microorganisms in the progress of periodontal diseases of OLP.

关键词： Aggregatibacter actinomycetemcomitans oral lichen planus Porphyromonas gingivalis Prevotel/a intermedia Tannerel/a forsythia Treponema denticola

Isolation and characterisation of human gingival margin-derived STRO-1/MACS^+ and MACS^-cell populations

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International Journal of Oral Science 2015年第2期7卷 80-88页

作者： Karim M Fawzy El-Sayed Sebastian Paris Christian Graetz Neemat Kassem Mohamed Mekhemar Hendrick Ungefroren Fred Fndrich Christof Drfer Clinic for Conservative dentistry and periodontology School of Dental MedicineChristian Albrechts University Oral Medicine and periodontology department Faculty of Oral and Dental MedicineCairo University department of Operative and preventive dentistry Charite-Universitatsmedizin Berlin department of Clinical Pathology Faculty of MedicineCairo University Clinic for Applied Cellular Therapy Christian Albrechts University

Recently,gingival margin-derived stem/progenitor cells isolated via STRO-1/magnetic activated cell sorting（MACS） showed remarkable periodontal regenerative potential in *** a second-stage investigation,the present study＇s aim was to perform in vitro characterisation and comparison of the stem/progenitor cell characteristics of sorted STRO-1-positive（MACS~＋） and STRO-1-negative（MACS^-） cell populations from the human free gingival *** were isolated from the free gingiva using a minimally invasive technique and were magnetically sorted using anti-STRO-1 ***,the MACS~＋ and MACS^- cell fractions were characterized by flow cytometry for expression of CD14,CD34,CD45,CD73,CD90,CD105,CD146/MUC18 and ***-forming unit（CFU） and multilineage differentiation potential were assayed for both cell *** marker expression was examined using real-time polymerase chain reaction（PCR）.MACS~＋ and MACS- cell fractions showed plastic ***~＋ cells,in contrast to MACS- cells,showed all of the predefined mesenchymal stem/progenitor cell characteristics and a significantly higher number of CFUs（P〈0.01）.More than 95%of MACS~＋ cells expressed CD105,CD90 and CD73;lacked the haematopoietic markers CD45,CD34 and CD14,and expressed STRO-1 and CD146/***- cells showed a different surface marker expression profile,with almost no expression of CD14 or STRO-1,and more than 95%of these cells expressed CD73,CD90 and CD146/MUC18,as well as the haematopoietic markers CD34 and CD45 and ***~＋ cells could be differentiated along osteoblastic,adipocytic and chondroblastic *** contrast,MACS- cells demonstrated slight osteogenic *** MACS~＋ cells showed significantly higher expression of collagen I（P〈0.05） and collagen III（P〈0.01）,whereas MACS^- cells demonstrated higher expression of osteonectin（P〈0.05;MannWhitney）.The present study is the first to compare gingival MACS~＋ and MACS- cell populations demo

关键词： gingival progenitor populations margin periodontal cytometry collagen isolation dental sorting