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Virologica Sinica 2024年第1期39卷 71-80页

作者： Mengmeng Cao Qiannan Jia Jinghua Li Lili Zhao Li zhu Yufan Zhang Shan Li Tao Deng National Institute of pathogen biology Chinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing100730China CAS Key Laboratory of pathogen microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijing100101China

The emergence of influenza virus A pandemic H1N1 in April 2009 marked the first pandemic of the 21st *** this study,we observed significant differences in the polymerase activities of two clinical 2009 H1N1 influenza A virus isolates from Chinese and Japanese *** comparison of the three main protein subunits(PB2,PB1,and PA)of the viral RNA-dependent RNA polymerase complex and subsequent mutational analysis revealed that a single amino acid substitution(E206K)was responsible for the observed impaired replication *** in vitro experiments showed that presence of PAE206K decreased the replication of influenza A/WSN/33 virus in mammalian cells and a reduction in the virus’s pathogenicity in *** studies revealed that PAE206K is a temperature-sensitive mutant associated with the inability to transport PB1–PA complex to the nucleus at high temperature(39.5℃).Hence,this naturally occurring variant in the PA protein represents an ideal candidate mutation for the development of live attenuated influenza vaccines.

关键词： H1N1 Influenza A virus Polymerase acidic protein Point mutation Viral replication

Baicalein suppresses Coxsackievirus B3 replication by inhibiting caspase-1 and viral protease 2A

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Virologica Sinica 2024年第4期39卷 685-693页

作者： Yanyan Dong Enze Shao Siwei Li Ruiqi Wang Dan Wang Lixin Wang Hong Yang Yingxia He Tian Luan Yang Chen Yao Wang Lexun Lin Yan Wang Zhaohua Zhong Wenran Zhao department of Cell biology Harbin Medical UniversityHarbin 150081China department of microbiology Harbin Medical UniversityHarbin 150081China

Myocarditis is an inflammatory disease of the cardiac muscle and one of the primary causes of dilated *** B coxsackievirus(CVB)is one of the leading causative pathogens of viral myocarditis,which primarily affects children and young *** to the lack of vaccines,the development of antiviral medicines is crucial to controlling CVB infection and the progression of *** this study,we investigated the antiviral effect of baicalein,a flavonoid extracted from Scutellaria *** results demonstrated that baicalein treatment significantly reduced cytopathic effect and increased cell viability in CVB3-infected *** addition,significant reductions in viral protein 3D,viral RNA,and viral particles were observed in CVB3-infected cells treated with *** found that baicalein exerted its inhibitory effect in the early stages of CVB3 *** also suppressed viral replication in the myocardium and effectively alleviated myocarditis induced by CVB3 *** study revealed that baicalein exerts its antiviral effect by inhibiting the activity of caspase-1 and viral protease *** together,our findings demonstrate that baicalein has antiviral activity against CVB3 infection and may serve as a potential therapeutic option for the myocarditis caused by enterovirus infection.

关键词： Baicalein Coxsackievirus B(CVB) Myocarditis 2A protease Caspase-1

Virus-like nanoparticle vaccines for inducing long-lasting immunity against infectious diseases

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National Science Review 2024年第4期11卷 13-15页

作者： Yi-Nan Zhang Sarah Auclair Jiang Zhu department of Integrative Structural and Computational biology The Scripps Research Institute department of Immunology and microbiology The Scripps Research Institute

Prophylactic vaccines that can generate long-lasting protective immune responses are needed for many infectious diseases. Efficient vaccination requires vaccine antigens to be retained and presented to B cells in lymp...

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Correlation between circulating T follicular helper cell levels after infection and a decreased risk of COVID-19 re-infection

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Virologica Sinica 2024年第4期39卷 699-701页

作者： Jinzhu Feng Zeyu Pu Rong Li Yuzhuang Li Xuewen Qin Hui Zhang Yiwen Zhang Institute of Human Virology Department of Pathogen Biology and BiosecurityKey Laboratory of Tropical Disease Control of Ministry of EducationZhongshan School of MedicineSun Yat-sen UniversityGuangzhou510080China

Dear Editor,As mass vaccination has been accomplished worldwide and a growing number of individuals have already been infected by SARS-CoV-2,understanding the incidence of subsequent re-infection is urgently required for comprehending the transmission dynamics of COVID-19(Bobrovitz et al.,2023).Additionally,current SARS-CoV-2 variants cause widespread escape from the neutralizing antibody(nAb)elicited by current ***,durable neutralization and memory B cell responses can be predicted by rapid CD4t T cell responses,particularly strong circulating T follicular helper(cTfh)cell responses,in individuals infected with SARS-CoV-2(Gong et al.,2020;Boppana et al.,2021;Narowski et al.,2022).Although nAb titers are known as predictors of protection against SARS-CoV-2 variants(Khoury et al.,2021),identifying more potential factors that could predict the risk of re-infection following primary infection and vaccination has become quite important in shaping prevention strategies(Chemaitelly et al.,2023).

关键词： infection prevention subsequent

Electrobiocorrosion by microbes without outer-surface cytochromes

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mLife 2024年第1期3卷 110-118页

作者： Dawn E.Holmes Trevor L.Woodard Jessica A.Smith Florin Musat Derek R.Lovley department of microbiology University of Massachusetts AmherstAmherstMassachusettsUSA department of Physical and Biological Science Western New England UniversitySpringfieldMassachusettsUSA department of Biomolecular Sciences Central Connecticut State UniversityNew BritainConnecticutUSA department of biology Section for MicrobiologyAarhus UniversityAarhusDenmark department of Molecular biology and Biotechnology Facuity of Biology and GeologyBabes-Bolyai UniversityCluj-NapocaRomania

Anaerobic microbial corrosion of iron-containing metals causes extensive economic *** microbes are capable of direct metal-to-microbe electron transfer(electrobiocorrosion),but the prevalence of electrobiocorrosion among diverse methanogens and acetogens is poorly understood because of a lack of tools for their genetic *** studies have suggested that respiration with 316L stainless steel as the electron donor is indicative of electrobiocorrosion,because,unlike pure Fe^(0),316L stainless steel does not abiotically generate H_(2) as an intermediary electron ***,we report that all of the methanogens(Methanosarcina vacuolata,Methanothrix soehngenii,and Methanobacterium strain IM1)and acetogens(Sporomusa ovata and Clostridium ljungdahli)evaluated respired with pure Fe^(0)as the electron donor,but only ***,***,and *** were capable of stainless steel *** electrobiocorrosive methanogens re-quired acetate as an additional energy source in order to produce methane from stainless *** of *** and *** demonstrated how acetogens can provide acetate to methanogens during *** only was Meth-anobacterium strain IM1 not capable of electrobiocorrosion,but it also did not accept electrons from Geobacter metal-lireducens,an effective electron-donating partner for direct interspecies electron transfer to all methanogens that can directly accept electrons from Fe^(0).The finding that ***,***,and *** are capable of electrobiocorrosion,despite a lack of the outer-surface c-type cytochromes previously found to be important in other electrobiocorrosive microbes,demonstrates that there are multiple microbial strategies for making electrical contact with Fe^(0).

关键词： acetogen corrosion Fe^(0) methanogen stainless steel

Studies of polyol production by the yeast Yarrowia lipolytica growing on crude glycerol under stressful conditions

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Carbon Resources Conversion 2024年第3期7卷 17-31,F0004页

作者： Eleni-Stavroula Vastaroucha Nikolaos G.Stoforos George Aggelis Seraphim Papanikolaou department of Food Science and Human Nutrition Agricultural University of Athens75 Iera Odos11855AthensGreece Unit of microbiology Department of BiologyUniversity of Patras26504 Rio PatrasGreece

Crude glycerol,the principal by-product of biodiesel production process,was employed as substrate by three wild-type Yarrowia lipolytica strains(ACA-YC 5030,LMBF 20 and NRRL Y-323).Stressful conditions(low pH value=2.0±0.3,low incubation temperature T=20±1℃,non-aseptic conditions)were ***-esting production of yeast biomass and polyols(***,mannitol and arabitol)was noted at pH=2.0±0.3 and T=20±1℃.Strains failed to produce significant quantities of cellular lipid,while variable quantities of intra-cellular polysaccharides were *** under previously pasteurized media supported significant biomass and polyols production for most of the tested strains,while only one strain(NRRL Y-323),managed to produce polyols at media that were not previously thermally treated at *** production of mannitol was favored at low initial glycerol(Glol0)concentrations,whereas higher Glol0 quantities favored the biosynthesis of *** the strain NRRL Y-323,highly aerated/agitated bioreactor trials showed different physiological profiles as compared to the respective flask ***,in flask experiments with the strain NRRL Y-323 at high Glol0 amounts(≈140 g/L)at low medium pH(=2.0±0.3),a significant production of polyols(=84.2 g/L)with the corresponding remarkable conversion yield on glycerol consumed=62%w/w was *** application:Renewable and biodegradable fuels,such as biodiesel,are safer and environmentally friendlier than the conventional petroleum *** is a cost-effective substrate obtained as the main side-product from biodiesel production process and is currently being employed in the realm of Industrial Microbi-ology and Biotechnology to produce metabolic products with added *** research focuses on using glycerol as a starting substrate for biotechnological conversions aiming at producing,amongst other compounds,polyols,microbial biomass,citric acid,*** selected strains of the Generall

关键词： Biomass Glycerol Polyols Yarrowia lipolytica

SOX11 as a potential prognostic biomarker in hepatocellular carcinoma linked to immune infiltration and ferroptosis

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Chinese Journal of Cancer Research 2024年第4期36卷 378-397页

作者： Hongyu Chen Qiangguo Ao Yueling Wang Yue Qian Qingli Cheng Wei Zhang department of Nephrology the Second Medical Center of PLA General HospitalNational Clinical Research Center for Geriatric DiseasesBeijing 100853China Clinical Laboratory the First Affiliated Hospital of Henan UniversityKaifeng 475000China Cell biology department Wuxi School of MedicineJiangnan UniversityWuxi 214122China department of pathogen biology Guizhou Nursing Vocational CollegeGuiyang 550000China

Objective:SOX11 is expressed in numerous malignancies,including hepatocellular carcinomas(HCC),but its oncogenic function has not been ***,we performed a comprehensive bioinformatics analysis of the Liver Hepatocellular Carcinoma(LIHC)dataset to investigate the function of SOX11 in ***:SOX11 expression data from The Cancer Genome Atlas(TCGA)and Gene Expression Omnibus(GEO)were validated by immunohistochemistry(IHC).Co-expression,differential expression,and functional analyses utilized TCGA-LIHC,Timer 2.0,Metascape,GTEx,and LinkedOmics *** with immune infiltration,ferroptosis,and immune checkpoint genes were *** changes were explored via *** regression,receiver operating characteristic curve(ROC),Kaplan-Meier analysis,and nomogram modeling evaluated associations with HCC clinicopathological ***11’s impact on proliferation and migration was studied in HepG2 and HuH7 cell ***:SOX11 was significantly elevated in HCC tumors compared to ***11-associated genes exhibited differential expression in pathways involving extracellular membrane ion *** associations were found between SOX11 levels,immune infiltration,ferroptosis,and immune checkpoint genes in HCC ***11 levels correlated with HCC stage,histologic grade,and tumor status,and independently predicted overall and disease-specific ***11 expression effectively distinguished between tumor and normal liver *** correlations highlighted a significant relationship between SOX11 and ferroptosis-associated *** SOX11 levels in HepG2 and HuH7 cells resulted in reduced proliferation and ***:SOX11 was found to represent a promising biomarker within HCC diagnosis and prognosis together with being a possible drug-target.

关键词： SOX11 biomarker HCC prognosis genetic alterations tumor-immune infiltration ferroptosis

Lecithin-cholesterol acyltransferase is a potential tumor suppressor and predictive marker for hepatocellular carcinoma metastasis

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World Journal of Gastrointestinal Oncology 2024年第8期16卷 3651-3671页

作者： Yan Li Li-Na Jiang Bo-Kang Zhao Mei-Ling Li Yi-Yun Jiang Yi-Si Liu Shu-Hong Liu Li Zhu Xin Ye Jing-Min Zhao department of Pathology and Hepatology The Fifth Medical Center of Chinese PLA General HospitalBeijing 100039China department of Hepatology Center of Infectious Diseases and Pathogen BiologyThe First Hospital of Jilin UniversityChangchun 130061Jilin ProvinceChina First department of Liver Disease Center Beijing Youan HospitalCapital Medical UniversityBeijing 100069China Key Laboratory of pathogenic microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China Savaid Medical School University of Chinese Academy of SciencesBeijing 100190China department of Pathology The Fifth Medical Center of Chinese PLA General HospitalBeijing 100039China

BACKGROUND Hepatocellular carcinoma(HCC)is a major cause of cancer mortality worldwide,and metastasis is the main cause of early recurrence and poor ***,the mechanism of metastasis remains poorly *** To determine the possible mechanism affecting HCC metastasis and provide a possible theoretical basis for HCC *** The candidate molecule lecithin-cholesterol acyltransferase(LCAT)was screened by gene microarray and bioinformatics *** expression levels of LCAT in clinical cohort samples was detected by quantitative realtime polymerase chain reaction and western *** proliferation,migration,invasion and tumor-forming ability were measured by Cell Counting Kit-8,Transwell cell migration,invasion,and clonal formation assays,*** formation was detected in nude mice after LCAT gene knockdown or *** immunohistochemistry for Ki67,E-cadherin,N-cadherin,matrix metalloproteinase 9 and vascular endothelial growth factor were performed in liver tissues to assess the effect of LCAT on *** set enrichment analysis(GSEA)on various gene signatures were analyzed with GSEA version *** machine-learning algorithms(random forest,support vector machine,and logistic regression)were applied to predict HCC metastasis in The Cancer Genome Atlas and GEO *** LCAT was identified as a novel gene relating to HCC metastasis by using gene microarray in HCC *** was significantly downregulated in HCC tissues,which is correlated with recurrence,metastasis and poor outcome of HCC *** analysis indicated that LCAT inhibited HCC cell proliferation,migration and invasion both in vitro and in *** data showed that LCAT was negatively associated with HCC size and metastasis(HCC size≤3 cm vs 3-9 cm,P9 cm,P<0.01;metastatic-free HCC vs extrahepatic metastatic HCC,P<0.05).LCAT suppressed the growth,migration and invasion of HCC cell lines via PI3K/A

关键词： Lecithin-cholesterol acyltransferase Tumor suppressor gene Hepatocellular carcinoma PI3K/AKT/mTOR Predicting model

A statistical approach to enhance the productivity of Streptomyces baarensis MH-133 for bioactive compounds

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Synthetic and Systems Biotechnology 2024年第2期9卷 196-208页

作者： Mohamed H.Kalaba Gamal M.El-Sherbiny Osama M.Darwesh Saad A.Moghannem Botany and microbiology department Faculty of Science(Boys)Al-Azhar UniversityCairo 11884Egypt Agricultural microbiology department National Research CentreDokkiCairoEgypt

The goal of this study was to use statistical optimization to change the nutritional and environmental conditions so that Streptomyces baarensis MH-133 could make more active *** trials were used to screen for critical variables influencing productivity using the Placket-Burman Design *** MH-133 is significantly influenced by elicitation,yeast extract,inoculum size,and incubation period in terms of antibacterial activity.A total of 27 experimental trials with various combinations of these factors were used to carry out the response surface technique using the Box-Behnken *** analyses revealed that the model was highly significant(p<0.001),with a lack-of-fit of 0.212 and a coefficient determination(R2)of ***,the model predicted that the response as inhibition zone diameter would reach a value of 27 *** optimal conditions,*** MH-133 produced 18.0 g of crude extract to each 35L and was purified with column *** active fraction exhibiting antibacterial activity was characterized using spectroscopic *** MIC and MBC values varied between 37.5 and 300μg/ml and 75 and 300μg/ml,*** conclusion,the biostatistical optimization of the active fraction critical variables,including environmental and nutritional conditions,enhances the production of bioactive molecules by Streptomyces species.

关键词： S.baarensis MH-133 Placket-Burman designBox-Behnken design ESKAPE Antibacterial activity

Cancer-associated fibroblasts express CD1d and activate invariant natural killer T cells under cellular stress

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Cellular & Molecular Immunology 2024年第1期21卷 91-94页

作者： Shengduo Pei Jonas Sjölund Yueyun Pan Kristian Pietras Mikael C.I.Karlsson department of microbiology Tumor and Cell BiologyKarolinska InstitutetStockholmSweden Division of Translational Cancer Research Department of Laboratory MedicineLund University Cancer CentreLund UniversityLundSweden

Invariant natural killer T(iNKT)cells are part of the family of unconventional T cells,and they recognize glycolipid antigens presented on the MHC class I like molecule CD1^([1]).In humans there are five CD1 molecules named CD1a to CD1e,whereas mice use only CD1d for lipid ***,iNKT cells can elicit both cytotoxicity similarly to conventional CD8 T cells and secrete cytokines that influence the adaptive immune response including providing direct T cell help to B cells^([2]).In the context of tumors,they can use both release of granzyme B and use Fas-mediated mechanisms for killing of tumor *** iNKT cells use a restricted TCR repertoire and CD1 molecules are non-heterogenous the risk of graft vs host disease is limited and thus these T cells have been an attractive option for off the shelf immunotherapy to treat cancer^([3]).

关键词： killer CD1d Cancer