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An efficient strategy for establishing a model of sensorineural deafness in rats

Neural Regeneration Research 2015年第10期10卷 1683-1689页

作者： Long Ma Hai-jin Yi Fen-qian Yuan Wei-wei Guo Shi-ming Yang The Second Artillery General Hospital of Chinese PLA department of otolaryngology-Head & Neck Surgery Beijing Tiantan Hospital Capital Medical University department of Head and Neck Surgery Jiangxi Cancer Hospital department of otolaryngology Head & Neck Surgery Institute of Otolaryngology Chinese PLA General Hospital

Ototoxic drugs can be used to produce a loss of cochlear hair cells to create animal models of deafness. However, to the best of our knowledge, there is no report on the establishment of a rat deafness model through the combined application of aminoglycosides and loop diuretics. The aim of this study was to use single or combined administration of furosemide and kanamycin sulfate to establish rat models of deafness. The rats received intravenous injections of different doses of furosemide and/or intramuscular injections of kanamycin sulfate. The auditory brainstem response was measured to determine the hearing threshold after drug application. Immunocytochemistry and confocal microscopy were performed to evaluate inner ear morphology. In the group receiving combined administration of furosemide and kanamycin, the auditory brainstem response threshold showed significant elevation 3 days after administration, higher than that produced by furosemide or kanamycin alone. The hair cells showed varying degrees of injury, from the apical turn to the basal turn of the cochlea and from the outer hair cells to the inner hair cells. The spiral ganglion cells maintained a normal morphology during the first week after the hair cells completely disappeared, and then gradually degenerated. After 2 months, the majority of spiral ganglion cells disappeared, but a few remained. These findings demonstrate that the combined administration of furosemide and kanamycin has a synergistic ototoxic effect, and that these drugs can produce hair cell loss and hearing loss in rats. These findings suggest that even in patients with severe deafness, electronic cochlear implants may partially restore hearing.

关键词： nerve regeneration sensorineural deafness kanamycin furosemide ototoxic drug spiral ganglion cells hair cell neural regeneration

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Role of adjunctive treatment strategies in COVID-19 and a review of international and national clinical guidelines

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Military Medical Research 2020年第2期7卷 209-227页

作者： Xin-Ni Xu Yew Kwang Ong De Yun Wang department of otolaryngology-Head and Neck Surgery National University Hospital SystemSingaporeSingapore department of otolaryngology Yong Loo Lin School of MedicineNational University of SingaporeSingaporeSingapore

The coronavirus disease(COVID-19)pandemic has led to a global struggle to cope with the sheer numbers of infected persons,many of whom require intensive care support or eventually succumb to the *** outbreak is managed by a combination of disease containment via public health measures and supportive care for those who are *** date,there is no specific anti-COVID-19 ***,the urgency to identify treatments that could turn the tide has led to the emergence of several investigational drugs as potential candidates to improve outcome,especially in the severe to critically *** many of these adjunctive drugs are being investigated in clinical trials,professional bodies have attempted to clarify the setting where the use of these drugs may be considered as off-label or compassionate *** review summarizes the clinical evidence of investigational adjunctive treatments used in COVID-19 patients as well as the recommendations of their use from guidelines issued by international and national organizations in healthcare.

关键词： COVID-19 Adjunctive treatment Chloroquine Lopinavir-ritonavir Remdesivir Corticosteroids Umifenovir Convalescent plasma

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Myricetin alleviates ovalbumin-induced allergic rhinitis in mice by regulating Th1/Th2 balance

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Asian Pacific Journal of Tropical Biomedicine 2023年第7期13卷 306-314页

作者： Ya-Nan Shi Jin-Zhu Su Juan Wang Jiang-Qiao Geng department of otolaryngology Sanya Women and Children's HospitalSanya572000China department of otolaryngology Hebei Children’s HospitalShijiazhuang050031China

Objective:To evaluate the effect of myricetin on ovalbumin(OVA)-induced allergic rhinitis in ***:Mice were sensitized and challenged using OVA(5%,500 mL)intraperitoneally and intranasally,respectively,on an alternative day for 14 days,followed by administration of myricetin(50,100,and 200 mg/kg)till day *** symptoms,biochemical parameters,protein expressions,and histopathology were ***:OVA-induced increased nasal symptoms including rubbing,sneezing,and discharge were significantly reduced by myricetin(100and 200 mg/kg)(P<0.05).Myricetin also protected against histamine challenge and attenuated elevated serum immunoglobulin E(IgE;total and OVA-specific),total IgG1,andβ-hexosaminidase levels,as well as leukotriene C4 and interleukins levels in nasal lavage fluid(P<0.05).Western blot analysis showed that myricetin significantly upregulated the protein expression of T-box expressed in T cells,while downregulating the protein expression of GATA binding protein 3,NF-κB,and IκB-α(P<0.05).Additionally,OVA-induced histopathological abberations in the nasal mucosa was markedly ameliorated by myricetin treatment(P<0.05).Conclusions:Myricetin exerts anti-allergic effects against OVAinduced allergic rhinitis via regulating Th1/Th2 balance.

关键词： Allergic rhinitis GATA3 Interleukins Myricetin Montelukast Ovalbumin Th1/Th2 balance

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Olfaction as a soldier——a review of the physiology and its present and future use in the military

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Military Medical Research 2017年第3期4卷 157-169页

作者： Palaniappan Ganesh Nagappan Somasundram Subramaniam De-Yun Wang School of Clinical Medicine University of Cambridge department of otolaryngology Ng Teng Fong General Hospital department of otolaryngology National University of Singapore National University Health System department of otolaryngology Yong Loo Lin School of Medicine National University of Singapore

Olfaction is one of our 5 main qualitative sensory abilities. In this review, we have examined the physiology of olfaction from the olfactory receptor to the brain. Through analyzing the physiology of olfaction, we have found that the biochemistry of olfactory nerve stimulation is unique from that of other similar pathways. Upon receiving large amounts of input from the olfactory nerve, the olfactory bulb, followed by several layers of centrifugal and centripetal processing in the brain, has to sort the information from the input as well as integrate it with other inputs from the brain to develop a coherent understanding of the input. We then examined the implications of olfaction in the military, the practical applications of electronic noses and problems associated with injury to olfaction that could affect compensation and combat worthiness of a soldier following injury. In the military, olfaction can allow the army to perform at its best through 4 main methods, namely ensuring olfaction is consistent with other dimensions of perception(ensuring optimal olfaction ability in all soldiers in combat), understanding the impact of different common combat environments on the sense of smell, utilizing odor as a defense mechanism and using olfactory aids when necessary. Electronic noses are olfactory aids that have a large potential in the military ranging from saving lives through the detection of explosives to potential methods for improving combustion efficiency. There are several problems associated with injury to olfaction that should be considered when deciding on compensation and combat worthiness of the soldier following an injury.

关键词： Olfaction Military Electronic nose Compensation Physiology Injury Combat

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Middle Ear Implants:Historical and futuristic perspective

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Journal of Otology 2011年第2期6卷 10-18页

作者： Guyan Andre Channer Adrien A Eshraghi department of otolaryngology University of Miami

Technological advancements are continuously changing the paradigm of human *** beings are constantly engaging in various measures to reduce the extent of sensory and motor *** has been in the form of various devices,*** prosthesis,visual aids (spectacles) and hearing *** attempts throughout the centuries have been made in an effort to improve sound amplification in *** article seeks to highlights the technological journey of one such implant,the middle ear implant,from its inception to the more technological advanced futuristic *** there are many amplification devices available presently,there still remains a group of patients who have not experienced adequate amplification for their hearing loss and this subset may gain the greatest benefit from middle ear implants.

关键词： middle ear implants vibrant sound bridge esteem envoy Rion E device hearing aids Totally Implantable Cochlear Amplifier middle ear transducer (MET) MAXUM

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Novel defined N7-methylguanosine modification-related lncRNAs for predicting the prognosis of laryngeal squamous cell carcinoma

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BIOCELL 2023年第9期47卷 1965-1975页

作者： ZHAOXU YAO HAIBIN MA LIN LIU QIAN ZHAO LONGCHAO QIN XUEYAN REN CHUANJUN WU KAILI SUN department of otolaryngology Handan Central HospitalHandan056001China

Objective:Through integrated bioinformatics analysis,the goal of this work was to find new,characterised N7-methylguanosine modification-related long non-coding RNAs(m7G-lncRNAs)that might be used to predict the prognosis of laryngeal squamous cell carcinoma(LSCC).Methods:The clinical data and LSCC gene expression data for the current investigation were initially retrieved from the TCGA database&***,using co-expression analysis of m7G-associated mRNAs&lncRNAs&differential expression analysis(DEA)among LSCC&normal sample categories,we discovered lncRNAs that were connected to *** prognosis prediction model was built for the training category using univariate&multivariate COX regression&LASSO regression analyses,&the model’s efficacy was checked against the test category *** addition,we conducted DEA of prognostic m7G-lncRNAs among LSCC&normal sample categories&compiled a list of co-expression networks&the structure of prognosis *** compare the prognoses for individuals with LSCC in the high-&low-risk categories in the prognosis prediction model,survival and risk assessments were also carried ***,we created a nomogram to accurately forecast the outcomes of LSCC patients&created receiver operating characteristic(ROC)curves to assess the prognosis prediction model’s predictive ***:Using co-expression network analysis&differential expression analysis,we discovered 774 m7G-lncRNAs and 551 DEm7G-lncRNAs,*** then constructed a prognosis prediction model for six m7G-lncRNAs(FLG−AS1,RHOA−IT1,AC020913.3,AC027307.2,AC010973.2 and AC010789.1),identified 32 DEPm7G-lncRNAs,analyzed the correlation between 32 DEPm7G-lncRNAs and 13 DEPm7G-mRNAs,and performed survival analyses and risk analyses of the prognosis prediction model to assess the prognostic performance of LSCC *** displaying ROC curves and a nomogram,we finally checked the prognosis prediction model's ***:By creating novel predictive

关键词： N7-methylguanosine modification Prognostic lncRNAs signatures Prognosis prediction model Laryngeal squamous cell carcinoma

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Expression of IL-17 and Syndecan-1 in Nasal Polyps and Their Correlation with Nasal Polyps

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2017年第3期37卷 412-418页

作者：龚国清任芳芳王彦君万浪陈删袁杰杨传梅刘邦华孔维佳 department of otolaryngology Union Hospital Tongji Medical College Huazhong University of Science and Technology department of otolaryngology The Fifth Affiliated Hospital of Zhengzhou University department of Otolarygology Huangshi Central Hospital

Nasal polyp(NP) is a common chronic inflammatory disease of the nasal cavity and sinuses. Although some authors have suggested that NP is related to inflammatory factors such as interleukin(IL)-1β, IL-5, IL-8, granulocyte-macrophage colony-stimulating factor(GM-CSF), tumor necrosis factor(TNF)-α, and IL-17, the mechanisms underlying the pathogenesis and progression of NP remain obscure. This study investigated the expression and distribution of IL-17 and syndecan-1 in NP, and explored the roles of these two molecules in the pathogenesis of eosinophilic chronic rhinosinusitis with nasal polyps(Eos CRSwNP) and non-Eos CRSwNP. Real-time PCR and immunohistochemistry were used to detect the expression of IL-17 and syndecan-1 in samples [NP, unciform process(UP) from patients with CRS, and middle turbinate(MT) from healthy controls undergoing pituitary tumor surgery]. The results showed that the expression levels of IL-17 and syndecan-1 were upregulated in both NP and UP tissues, but both factors were higher in NP tissues than in UP tissues. There was no significant difference in IL-17 levels between the Eos CRSw NP and non-Eos CRSwNP samples, and syndecan-1 levels were increased in the non-Eos CRSwNP tissues as compared with those in Eos CRSwNP tissues. In all of the groups, there was a close correlation between the expression of IL-17 and syndecan-1 in nasal mucosa epithelial cells, glandular epithelial cells, and inflammatory cells, suggesting that IL-17 and syndecan-1 may play a role, and interact with each other, in the pathogenesis of non-Eos CRSwNP.

关键词： interleukin-17 syndecan-1 nasal polyps

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Comparison of human nasal epithelial cells grown as explant outgrowth cultures or dissociated tissue cultures in vitro

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Frontiers of Medicine 2013年第4期7卷 486-491页

作者： Jian Jiao Na Meng Hong Wang Luo Zhang department of otolaryngology Head and Neck SurgeryBeijing Tongren HospitalCapital Medical UniversityBeijing 100005ChinaKey Laboratory of OtolaryngologyHead and Neck Surgery(Ministry of Education)Beijing Institute of OtolaryngologyBeijing 100005China

The purpose of this study was to compare cell growth characteristics,ciliated cell differentiation,and function of human nasal epithelial cells established as explant outgrowth cultures or dissociated tissue *** nasal mucosa of the uncinate process was obtained by endoscopy and epithelial cell cultures were established by explant outgrowth or dissociated tissue culture *** cell growth characteristics were observed by inverted phase contrast *** cell differentiation was detected byβ-tubulin IV and ZO-1 *** and ATP-stimulated ciliary beat frequency(CBF)was measured using a high-speed digital microscopic imaging *** the explant and dissociated tissue cultures established as monolayers with tight junctions and differentiated cell composition,with both types of cultures comprising ciliated and non-ciliated epithelial *** were also frequently found in explant cultures but rarely seen in dissociated tissue *** both culture systems,the highest ciliated cell density appeared at 7th–10th culture day and declined with time,with the lifespan of ciliated cells ranging from 14 to 21 ***,10%of the cells in explant cultures and 20%of the cells in the dissociated tissue cultures were *** two cultures demonstrated similar ciliary beat frequency values at baseline(7.78±1.99 Hz and 7.91±2.52 Hz,respectively)and reacted equivalently following stimulation with 100μM *** results of this study indicate that both the explant outgrowth and dissociated tissue culture techniques are suitable for growing well-differentiated nasal ciliated and non-ciliated cells,which have growth characteristics and ciliary activity similar to those of nasal epithelial cells in vivo.

关键词： ciliated cells ciliary beat frequency dissociated tissue culture explant culture nasal epithelial cells

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Analysis of Etiology,Diagnosis,and Treatment Strategy and Efficacy of Pulsatile Tinnitus Caused by Abnormal Vascular Anatomy

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Current Medical Science 2023年第1期43卷 173-183页

作者： Sha LV Jia-tian YUAN Qi-wei XIE Shi-xiong TANG Yao-wen WANG department of otolaryngology Ningbo First HospitalNingbo315000China

Objective To investigate the etiology,clinical characteristics,diagnosis,and treatment strategies and efficacy of pulsatile tinnitus(PT)caused by vascular anatomy *** The clinical data of 45 patients with PT in our hospital from 2012 to 2019 were collected and retrospectively *** All 45 patients had vascular anatomical *** patients were divided into 10 categories according to the different locations of vascular abnormalities:sigmoid sinus diverticulum(SSD),sigmoid sinus wall dehiscence(SSWD),SSWD with high jugular bulb,pure dilated mastoid emissary vein,aberrant internal carotid artery(ICA)in the middle ear,transverse-sigmoid sinus(TSS)transition stenosis,TSS transition stenosis with SSD,persistent occipital sinus stenosis,petrous segment stenosis of ICA,and dural arteriovenous *** patients complained of PT synchronous with heartbeat *** interventional therapy and extravascular open surgery were used according to the location of the vascular *** disappeared in 41 patients,was significantly relieved in 3 patients,and was unchanged in 1 patient *** for one patient with transient headache postoperatively,no obvious complications *** PT caused by vascular anatomy abnormalities can be identified by detailed medical history and physical and imaging *** can be relieved or even completely alleviated after appropriate surgical treatments.

关键词： pulsatile tinnitus vessel structural abnormity etiology treatment strategy efficacy

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Intra-operative hearing monitoring methods in middle ear surgeries

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Journal of Otology 2016年第4期11卷 -页

作者： Wei Ren Fei Ji Jialing Zeng Hui Zhao department of otolaryngology/Head and Neck Surgery Chinese PLA Institute of OtolaryngologyChinese PLA General Hospital

Hearing loss is a condition affecting millions of people worldwide. Conductive hearing loss (CHL) is mainly caused by middle ear diseases. The low frequency area is the pivotal part of speech frequencies and most frequently impaired in patients with CHL. Among various treatments of CHL, middle ear surgery is efficient to improve hearing. However, variable success rates and possible needs for prolonged revision surgery still frustrate both surgeons and patients. Nowadays, increasing numbers of researchers explore various methods to monitor the efficacy of ossicular reconstruction intraoperatively, including electrocochleography (ECochG), auditory brainstem response (ABR), auditory steady state response (ASSR), distortion product otoacoustic emissions (DPOAE), subjective whisper test, and optical coherence tomography (OCT). Here, we illustrate several methods used clinically by reviewing the literature.

关键词： Intraoperative monitoring Middle ear surgery Hearing

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