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Resolving the developmental mechanisms of cardiac microthrombosis of SARS-CoV-2 based on single-cell transcriptome analysis

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Science China Life Sciences 2025年第1期68卷 103-115页

作者： Xizi Luo Nan Zhang Yuntao Liu Beibei Du Xuan Wang Tianxu Zhao Bingqiang Liu Shishun Zhao Jiazhang Qiu Guoqing Wang State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious diseases Key Laboratory for Zoonosis Research of the Ministry of EducationCollege of Basic MedicineJilin University State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious diseases Key Laboratory for Zoonosis Research of the Ministry of EducationCollege of Veterinary MedicineJilin University College of Mathematics Jilin University Bioinformatic Lab School of MathematicsShandong University department of Cardiology China-Japan Union HospitalJilin University

The coronavirus disease 2019（COVID-19） outbreak caused by the severe acute respiratory syndrome coronavirus-2（SARS-CoV-2） developed into a global health emergency. Systemic microthrombus caused by SARS-CoV-2 infection is a common complication in patients with COVID-19. Cardiac microthrombosis as a complication of SARS-CoV-2 infection is the primary cause of cardiac injury and death in patietns with severe COVID-19. In this study, we performed single-cell sequencing analysis of the right ventricular free wall tissue from healthy donors,patients who died during the hypercoagulable period of characteristic coagulation abnormality（CAC）, and patients who died during the fibrinolytic period of CAC. We collected 61,187 cells enriched in 24 immune cell subsets and 13 cardiac-resident cell subsets. We found that in the course of SARS-CoV-2 infected heart microthrombus, MYO1EhighRASGEF1Bhighmonocyte-derived macrophages promoted hyperactivation of the immune system and initiated the extrinsic coagulation pathway by activating chemokines CCL3, CCL5. This series of events is the main cause of cardiac microthrombi following SARS-CoV-2 infection. In a SARS-CoV-2 infected heart microthrombus, excessive immune activation is accompanied by an increase in cellular iron content, which in turn promotes oxidative stress and intensifies intercellular competition. This induces cells to alter their metabolic environment, resulting in increased sugar uptake via the glycosaminoglycan synthesis pathway. In addition, high levels of reactive oxygen species generated by elevated iron levels promote increased endogenous malondialdehyde synthesis in a subpopulation of cardiac endothelial cells. This exacerbates endothelial cell dysfunction and exacerbates the coagulopathy process.

关键词： SARS-CoV-2 cardiac microthrombosis immune hyperactivation cell competition

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Effects of Selenium and Zinc on Renal Oxidative Stress and Apoptosis Induced by Fluoride in Rats

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Biomedical and Environmental Sciences 2006年第6期19卷 439-444页

作者： RI-AN YU TAO XIA AI-GUO WANG XUE-MIN CHEN department of occupational and Environmental health School of Public Health Guangdong Pharmaceutical University Guangzhou 510310 Guangdong China department of occupational and Environmental health MOE Key Laboratory of Environment and Health School of Public Health Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 Hubei China

Objective To study the effects of selenium and zinc on oxidative stress, apoptosis, and cell cycle changes in rat renal cells induced by fluoride. Methods Wistar rats were given distilled water containing sodium fluoride （50 mg/L NaF） and were gavaged with different doses of selenium-zinc preparation for six months. Four groups were used and each group had eight animals （four males and four females）. Group one, sham-handled control; group two, 50 mg/L NaF; group three, 50 mg/L NaF with a low dose of selenium-zinc preparation （0.1 mg/kg Na2 SeO3 and 14.8 mg/kg ZnSO4 · 7H2O）; and group four, 50 mg/L NaF with a high dose of selenium-zinc preparation （0.2 mg/kg Na2 SeO3 and29.6 mg/kg ZnSO4 · 7H20）. The activities of serum glutathione peroxidase （GSH-Px）, kidney superoxide dismutase （SOD）, and the levels of malondialdehyde （MDA） and glutathione （GSH） in the kidney were measured to assess the oxidative stress. Kidney cell apoptosis and cell cycle were detected by flow cytometry. Results NaF at the dose of 50 mg/L increased excretion of fluoride in urine, promoted activity of urine γ -glutarnyl transpeptidase （ γ -GT）, inhibited activity of serum GSH-PX and kidney SOD, reduce kidney GSH content, and increased kidney MDA. NaF at the dose of 50 mg/L also induced rat renal apoptosls, reduced the cell number of G2/M phase in cell cycle, and decreased DNA relative content significantly. Selenium and zinc inhibited effects of NaF on oxidative stress and apoptosis, promoted the cell number of G2/M phase in cell cycle, but failed to increase relative DNA content significantly. Conclusion Sodium fluoride administered at the dose of 50 mg/L for six months induced oxidative stress and apoptosis, and changes the cell cycle in rat renal cells. Selenium and zinc antagonize oxidative stress, apoptosis, and cell cycle changes induced by excess fluoride.

关键词： Fluoride Selenium Zinc Oxidative stress Apoptosis Proliferation

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Development of Job Burden-capital Model of occupational Stress： An Exploratory Study

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Biomedical and Environmental Sciences 2016年第9期29卷 678-682页

作者： WANG Chao LI Shuang LI Tao YU Shan Fa DAI Jun Ming LIU Xiao Man ZHU Xiao Jun National Institute of occupational health and Poison Control Chinese Center for Disease Control and Prevention Beijing 200050 China Graduate School of Chinese Center for Disease Control and Prevention Beijing 202206 China department of occupational health Surveillance Prevention and Treatment Center for Occupational Diseases Zhengzhou 450052 Henan China School of public health Fudan University Shanghai 200032 China

This study proposes a new framework as job burden-capital model for comprehensive assessment on occupational stress. 1618 valid samples were recruited from electronic manufacturing service industry in Hunan province by self-rated questionnaires after written consent. Structural equation model analysis was employed to verify the model by the data collected. The final fitting model has satisfactory fitting goodness （CMIN/DF=5.78, AGFI=0.937, NNFI=0.960, IF1=0.968, RMSEA=0.054）. Both of the measurement model and structural model have acceptable path Ioadings. Job burden and capital could either directly affect occupational outcomes or indirectly influence them through equation personality.

关键词： Development of Job Burden-capital Model of occupational Stress

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Coincidence between malignant perivascular epithelioid cell tumor arising in the gastric serosa and lung adenocarcinoma

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World Journal of Gastroenterology 2015年第4期21卷 1349-1356页

作者： Sohsuke Yamada Atsunori Nabeshima Hirotsugu Noguchi Aya Nawata Hisae Nishii Xin Guo Ke-Yong Wang Masanori Hisaoka Toshiyuki Nakayama department of Pathology and Cell Biology School of MedicineUniversity of Occupational and Environmental HealthKitakyushu 807-8555Japan department of Urology School of MedicineUniversity of Occupational and Environmental HealthKitakyushu807-8555Japan Bio-information Research Center School of MedicineUniversity of Occupational and Environmental HealthKitakyushu 807-8555Japan department of Pathology and Oncology School of MedicineUniversity of Occupational and Environ-mental HealthKitakyushu 807-8555Japan

A 4-mo history of both epigastralgia and back pain was presented in a 39-year-old male. Computed tomography showed right lung nodule and abdominal mass attached to the gastric wall, measuring approximately 30 mm and 70 mm in diameter. Since biopsy samples from the lung and abdomen revealed poorly differentiated adenocarcinoma and malignant tumor, clinicians first interpreted the abdominal mass as metastatic carcinoma, and a right lower lobectomy with following resection of the mass was performed. Gross examination of both lesions displayed gray-whitish to yellow-whitish cut surfaces with hemorrhagic and necrotic foci, and the mass attached to the serosa of the lesser curvature on the gastric body. On microscopic examination, the lung tumor was composed of a proliferation of highly atypical epithelial cells having abundant eosinophilic cytoplasm, predominantly arranged in an acinar or solid growth pattern with vessel permeation, while the abdominal tumor consisted of sheets or nests with markedly atypical epithelioid cells having pleomorphic nuclei and abundant eosinophilic to clear cytoplasm focally in a radial perivascular or infiltrative growth pattern. Immunohistochemically, the latter cells were positive for HMB45 or α-smooth muscle actin, but the former ones not. Therefore, we finally made a diagnosis of malignant perivascular epithelioid cell tumor(PEComa) arising in the gastric serosa, combined with primary lung adenocarcinoma. Furthermore, small papillary car-cinoma of the thyroid gland was identified. The current case describes the coincidence of malignant PEComa with other carcinomas, posing a challenge in distinction from metastatic tumor disease.

关键词： Perivascular epithelioid cell tumor Malignant Gast

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Modulation of Behavior and Glutamate Receptor mRNA Expression in Rats after Sub-chronic Administration of Benzo(a)pyrene

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Biomedical and Environmental Sciences 2011年第4期24卷 408-414页

作者： TANG Qian XIA YinYin CHENG ShuQun TU BaiJie department of occupational and Environmental Medicine College of Public Health Chongqing MedicalUniversity Chongqing 400016 Sichuan China

Objective The present study aimed to test whether exposure to benzo（a）pyrene [B（a）P] affects spatial learning and short-term memory by modulating the expression of the Gria1 and Grin2a glutamate receptor subunit genes in the *** Thirty-six 21-24-day-old,male rats were randomly assigned into high-,medium-,and low-dose toxin exposure groups （6.25,2.5,and 1 mg/kg,respectively） and a control group,each containing nine *** behavioral performance of adult rats exposed to sub-chronic administration of B（a）P was monitored by learning and memory tests （Morris water maze）.Real-time PCR assays were used to quantify Gria1 and Grin2a gene expression in the *** At medium and high doses,B（a）P impaired spatial learning *** crossing-platform-location frequency and the time spent swimming in the platform area,which both relate to short-term memory,were significantly decreased in B（a）P-treated rats compared with *** level of Gria1 mRNA increased 2.6-5.9-fold,and the level of Grin2a mRNA increased 10-14.5-fold,with a greater fold increase associated with higher doses of B（a）*** We demonstrated that sub-chronic administration of B（a）P inhibits spatial learning and short-term memory,and increases Gria1 and Grin2a expression in the *** suggests a relationship of B（a）P exposure levels with Gria1 and Grin2a expression and impairment of short-term and spatial memory.

关键词： Benzo(a)pyrene Gria1 Grin2a

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Effects of Maternal Marginal Iodine Deficiency on Interactions between Cerebellar Bergmann Glia Cells and Purkinje Cells in Rat Offspring

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Biomedical and Environmental Sciences 2017年第12期30卷 932-937页

作者： YU Ye DONG Jing WANG Yuan WANG Yi MIN Hui SHAN Zhong Yan TENG Wei Ping CHEN Jie department of occupational and Environmental health School of Public Health China Medical University department of Endocrinology and Metabolism Liaoning Provincial Key Laboratory of Endocrine Diseases the First Hospital of China Medical University

Iodine deficiency （ID） during early pregnancy has an adverse effect on children＇s psychomotor and motor function but the mechanism has not been clarified. Therefore, our aim was to study the effect of maternal marginal ID on cerebellar neurodevelopment and the underlying mechanism. After obtaining marginal ID rats, we examined interactions between Bergmann gila cells （BGs） and Purkinje cells （PCs） using immunofluorescence and expression of the glutamate transporter and receptor by western blot. Our results showed that marginal ID reduced the number of contacted points between BGs and PCs,

关键词： Effects of Maternal Marginal Iodine Deficiency Cerebellar Bergman Purkinje Cells

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Enhancing Sound Absorption in Micro-Perforated Panel and Porous Material Composite in Low Frequencies:A Numerical Study Using FEM

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Sound & Vibration 2024年第1期58卷 81-100页

作者： Mohammad Javad SheikhMozafari department of occupational health Engineering School of Public HealthTehran University of Medical SciencesTehranIran

Mitigating low-frequency noise poses a significant challenge for acoustic engineers,due to their long wavelength,with conventional porous sound absorbers showing limitations in attenuating such *** effective strategy involves combining porous materials with micro-perforated plates(MPP)to address this *** the significant impact of structural variables like panel thickness,hole diameter,and air gap on the acoustic characteristics of MPP,achieving the optimal condition demands numerous sample *** impedance tube’s considerable expense for sound absorption measurement and the substantial cost involved in fabricating each sample using a 3D printer underscore the advantage of utilizing simulation methods to attain the optimal *** study focuses on optimizing low-frequency enhancement by investigating key *** the Finite Element Numerical Method(FEM)in COMSOL software,a composite panel was constructed comprising date palm fiber layers,an intervening air layer,and *** study explored the arrangement of these layers and the impact of parameters like hole diameter,plate thickness,and perforation ratio on acoustic *** selected optimal parameter at each stage was consistently maintained for subsequent *** revealed that layer arrangement significantly influenced acoustic *** the MPP layer before the porous material yielded superior low-frequency *** low-frequency behavior involved reducing hole diameter and perforation ratio while increasing plate *** the porous material’s thickness relative to the air layer behind the MPP enhanced absorption peak and resonance *** conclusion,halving the porous layer’s thickness while incorporating an air layer and single MPP proved more effective than using a thick porous *** approach not only reduces costs and space requirements but also enhances low-frequency *** study highlights the precision of

关键词： Micro-perforated panel date palm natural fibers low and middle frequency noise finite element method

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Arsenic exposure and glutamate-induced gliotransmitter release from astrocytes

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Neural Regeneration Research 2012年第31期7卷 2439-2445页

作者： Yan Wang Fenghong Zhao Yingjun Liao Yaping Jin Guifan Sun department of occupational and Environmental health School of Public Health China Medical University Shenyang 110001 LiaoningProvince China department of occupational and Environmental health School of Public Health Shenyang Medical College Shenyang 110034 LiaoningProvince China department of Physiology China Medical University Shenyang 110001 Liaoning Province China

The present study used cultures of primary astrocytes, isolated from neonatal rats, to verify the hypothesis that arsenite-induced neurotoxicity can influence neuronal function by altering glutamate-induced gliotransmitter release. Primary astrocytes were exposed to 0, 2.5, 5, 10, 20 or 30 μM arsenite for 24 hours. Cell viability and morphological observations revealed that 5 μM arsenic exposure could induce cytotoxicity. Cells were then cultured in the presence of 0, 2.5, 5, or 10 μM arsenite for 24 hours and stimulated with 25 μM glutamate for 10 minutes. Results showed that [Ca2＋]i in astrocytes exposed to 5 and 10 μM arsenite was significantly increased and levels of D-serine, γ-aminobutyric acid and glycine in cultures exposed to 2.5-10 μM arsenite were also increased. However, glutamate levels in the media were significantly increased only after treatment with 10 μM arsenite. In conclusion, our findings suggest that arsenic exposure may affect glutamate-induced gliotransmitter release from astrocytes and further disturb neuronal function.

关键词： arsenite astrocyte glutamate neuron cell viability intracellular free calcium gliotransmitter neurotoxicity neural regeneration

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Concentrations of disinfection by-products in swimming pool following modifications of the water treatment process:An exploratory study

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Journal of Environmental Sciences 2017年第8期29卷 163-172页

作者： Robert Tardif Manuel Rodriguez Cyril Catto Ginette Charest-Tardif Sabrina Simard department of Environmental and occupational health School of Public HealthUniversité de Montréal NSERC Industrial Research Chair on Drinking Water Université Laval

The formation and concentration of disinfection by-products（DBPs） in pool water and the ambient air vary according to the type of water treatment process used. This exploratory study was aimed at investigating the short-term impact of modifications of the water treatment process on traditional DBP levels（e.g., trihalomethanes（THMs）, chloramines） and emerging DBPs（e.g., Halonitromethanes, Haloketones, NDMA） in swimming pool water and/or air. A sampling program was carried to understand the impact of the following changes made successively to the standard water treatment process： activation of ultraviolet（UV）photoreactor, halt of air stripping with continuation of air extraction from the buffer tank,halt of air stripping and suppression of air extraction from the buffer tank, suppression of the polyaluminium silicate sulfate（PASS） coagulant. UV caused a high increase of Halonitromethanes（8.4 fold）, Haloketones（2.1 fold）, and THMs in the water（1.7 fold） and, of THMs in the air（1.6 fold） and contributed to reducing the level of chloramines in the air（1.6fold） and NDMA in the water（2.1 fold）. The results highlight the positive impact of air stripping in reducing volatile contaminants. The PASS did not change the presence of DBPs, except for the THMs, which decrease slightly with the use of this coagulant. This study shows that modifications affecting the water treatment process can rapidly produce important and variable impacts on DBP levels in water and air and suggests that implementation of any water treatment process to reduce DBP levels should take into account the specific context of each swimming pool.

关键词： Disinfection by-products Swimming pool UV rays Air stripping Chloramines Emerging DBPs NDMA

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Effects of Exposure to Trinitrotoluene on Male Reproduction

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Biomedical and Environmental Sciences 1993年第2期6卷 154-160页

作者： LI YI, JIANG QUAN-GUAN, YAO SHOU-QI, LIU WEI, TIAN GAO-JUN,AND GUI JING-WEIdepartment of occupational health, School of Public health, Beijing Medical University, Beijing ^Hebi Institute of occupational health, Henan Province Anyang Institute of occupational health, Henan Province Xinxiang Institute of occupational health, Henan Province, China Prof. Jiang Quan-guan, department of occupational health, School of Public health, Beijing Medical University, Beijing 100083 China. department of occupational health School of Public Health Beijing Medical University China.

A cross-sectional study was performed in 2 plants located in Henan Province in 1990 for observing the reproductive and sexual functions of male workers exposed to trinitrotoluene (TNT). The TNT concentrations in the workplace air, except the packing site, were found to have exceeded MAC (1 mg/m3). TNT exposed male workers complained of more sexual disorders such as impotence, the loss of libido and sexual hypoesthesia than the control group. Compared with the control group, the volume of semen and percentage of motile spermatozoawere found to have significantly decreased, and the sperm malformation incidence increased significantly in TNT exposed workers. The serum testosterone content in TNT exposed male workers was significantly decreased too.

关键词： TNT

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