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Human mesenchymal stromal cells enhance the immunomodulatory function of CD8＋CD28- regulatory T cells

cellular & molecular Immunology 2015年第6期12卷 708-718页

作者： Qiuli Liu Haiqing Zheng Xiaoyong Chen Yanwen Peng Weijun Huang Xiaobo Li Gang Li Wenjie Xia Qiquan Sun an Andy Peng Xiang cell-gene Therapy Translational Medicine Research Center The Third Affiliated Hospital Zhongshan School of Medicine Sun Yat-sen University Guangzhou Guangdong China Center for Stem cell Biology and Tissue Engineering The Key Laboratory for Stem Cells and Tissue Engineering Ministry of Education Sun Yat-sen University Guangzhou China department of Rehabilitation Medicine The Third Affiliated Hospital Sun Yat-sen University Guangzhou China Institute of Blood Transfusion Guangzhou Blood Centre Guangzhou China department of Renal Transplantation The Third Affiliated Hospital Sun Yat- sen University Guangzhou China department of Biochemistry Zhongshan Medical School Sun Yat-sen University Guangzhou China These authors contributed enuallv tn this wnrk

One important aspect of mesenchymal stromal cells （MSCs）-mediated immunomodulation is the recruitment and induction of regulatory T （Treg） cells. However, we do not yet know whether MSCs have similar effects on the other subsets of Treg cells. Herein, we studied the effects of MSCs on CD8＋CD28- Treg cells and found that the MSCs could not only increase the proportion of CD8＋CD28- T cells, but also enhance CD8＋CD28-T cells＇ ability of hampering naive CD4＋ T-cell proliferation and activation, decreasing the production of IFN-γ by activated CD4＋ T cells and inducing the apoptosis of activated CD4＋ T cells. Mechanistically, the MSCs affected the functions of the CD8＋CD28- T cells partially through moderate upregulating the expression of IL-10 and FasL. The MSCs had no distinct effect on the shift from CD8＋CD28＋ T cells to CD8＋CD28- T cells, but did increase the proportion of CD8＋CD28- T cells by reducing their rate of apoptosis. In summary, this study shows that MSCs can enhance the regulatory function of CD8＋CD28- Treg cells, shedding new light on MSCs-mediated immune regulation.

关键词： antigens CD28 CD8-positive T cells mesenchymal stromal cells regulatory T cells

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Hyperactivity of a midbrain dopamine to 5-HT circuit causes anorexia

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Journal of molecular cell Biology 2022年第5期14卷 63-64页

作者： Hailan Liu Xing Cai Yanlin He Yong Xu department of Pediatrics Children's Nutrition Research CenterBaylor College of MedicineHoustonTX 77030USA Kunming Institute of Zoology Chinese Academy of SciencesKunming 650201China Brain Glycemic and Metabolism Control department Pennington Biomedical Research CenterLouisiana State UniversityBaton RougeLA 70808USA department of molecular and cellular Biology Baylor College of MedicineHoustonTX 77030USA

Anorexia nervosa(AN)is a severe eating disorder that can eventually lead to death,buteffective the rapiesare missing dueto a lack of knowledge about its ***(DA)neurons in theventral tegmental area(VTA)and serotonin(5-HT)neurons in the dorsal Raphe nucleus(DRN)play essential roles in the control of eating and have been reported to be as sociated with human AN(Zhou and Palmiter,1995;Xu et al.,2017;Watson et al.,2019;He et al.,2021).

关键词： al. Watson dopamine

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Ego3 functions as a homodimer to mediate the interaction between Gtr1-Gtr2 and Egol in the EGO complex to activate TORC1

Ego3 functions as a homodimer to mediate the interaction bet...

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生命的分子机器及其调控网络——2012年全国生物化学与分子生物学学术大会

作者： Tianlong Zhang Marie-Pierre Pli-Gulli Hui Yang Claudio De Virgilio Jianping Ding State Key Laboratory of molecular Biology Institute of Biochemistry and Cell BiologyShanghai Institutes for Biological SciencesChinese Academy of Sciences Division of Biochemistry Department of BiologyUniversity of FribourgCH-1700 FribourgSwitzerland

TORC1 integrates a variety of cell growth signals to regulate cell growth,proliferation,and *** yeast EGO complex(EGOC),consisting of Gtr1,Gtr2,Ego1/Meh1,and Ego3/Slm4,localizes to the endosomal and vacuolar membranes and plays a pivotal role in cell growth and autophagy regulation through relaying amino acid signals to activate *** solved the crystal structures of a wild-type and a mutant form of S. cerevisiae Ego3 at 2.1 A and 2.6 A resolution,***3 assumes a homodimeric structure that displays

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Post-induction requirement of NMDA receptors for late phase long-term potentiation in vivo

Post-induction requirement of NMDA receptors for late phase ...

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中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Mu-Ming POO Institute of Neuroscience State Key Laboratory of NeuroscienceShanghai Institutes for Biological SciencesChinese Academy of Sciences Division of Neurobiology Department of Molecular and Cell BiologyUniversity of CaliforniaBerkeleyCA 94720-3200USA

Objective Persistent activity-induced long-term synaptic potentiation or depression(LTP or LTD) is regarded as the cellular basis for developmental refinement of neural circuits and for learning and memory in the adult *** temporal development of LTP includes an early-phase LTP(E-LTP;lasting 1-2 h) and a late-phase LTP(L-LTP;lasting > 3 h).However,the majority of our understanding on their properties and mechanisms comes from in vitro systems that are relatively silent concerning the spontaneous neural *** molecular and cellular mechanisms for the maintenance and reversal of LTP in the intact system with higher rate of natural spontaneous activities remain largely *** the present study,we have examined the consolidation of LTP at developing retinotectal synapses in Xenopus tadpoles in *** The field excitatory postsynaptic potentials(fEPSPs) were recorded in the tectum with a glass microelectrode filled with the HEPES-buffered bath saline,and its initial slope(20-80%of rising phase) is used to assay the long-term changes in the efficacy of developing retinotectal ***(1) Single episode of theta burst stimulation(TBS) to the optic nerve induced early-LTP of retinotectal synapses,lasting less than 1 h.(2) Three episodes of TBS with an optimal interval of 5 min(3×TBS-S5’) resulted in a robust and stable L-LTP lasting > 3 h.(3) In addition to the established role of activation of N-methyl-D-aspartate(NMDA) receptors during the induction,NMDA receptor activation and the subsequent intracellular Ca increase in postsynaptic cells within a critical time window of 30 min following 3×TBS-S5’are also critically required for the stabilization of L-LTP in *** Our results suggest that the post-induction activation of NMDA receptors and Ca signaling in a limited time window following spaced patterns of synaptic activity are critical for the L-LTP maintenance in vivo.

关键词： L-LTP TBS time window LTP maintenance NMDA receptors spontaneous activity

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Habitat preferences of two sparrow species are modified by abundances of other birds in an urban environment

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Current Zoology 2016年第4期62卷 357-368页

作者： Piotr SKoRKA Katarzyna S,ERPOWSKA Andzelika HAIDT Lukasz MYCZKO Anna EKNER-GRZYB Zuzanna M. ROSIN Zbigniew KWlECNsKI Joanna SUCHODOLSKA Viktoria TAKACS Lukasz JANKOWIAK Oskar WASIELEWSKI Agnieszka GRACLIK Agata J. KRAWCZYK Adam KASPRZAK Przemystaw SZWAJKOWSKI Przemystaw WYLEGAlA Anna W. MALECHA Tadeusz MIZERA Piotr TRYJANOWSKI Institute of Nature Conservation Polish Academy of Sciences AI. Mickiewicza 33 31-120 Krakow Institute of Zoology Poznan University of Life Sciences Wojska Polskiego 71 C 66-625 Poznan department of Forest Ecology Forest Research Institute Braci Lesej Sekocin Stary 05-090 Raszyn Oepartment of Behavioural Ecology department of cell Biology Faculty of Biology Adam Mickiewicz University Umultowska 89 61-614 Poznan Zoological Garden Browarna 25 61-063 Poznan department of Vertebrate Zoology and Anthropology University of Szczecin Wa ska 13 71-412 Szczecin department of Systematic Zoology Faculty of Biology Adam Mickiewicz University Umultowska 89 61-614 Poznan Division of Rural Tourism Poznad University of Life Sciences Witosa 45/114b 61-693 Poznan department of Animal physiology and Biochemistry Poznan University of Life Sciences Wolynska 35 60-637 Poznan Polish Society for Nature Conservation Salamandra Stolarska 7/3 60-788 Poznan Poland

Every species has certain habitat requirements, which may be altered by interactions with other co-occurring species. These interactions are mostly ignored in predictive models trying to identify key habitat variables correlated with species population abundance/occurrence. We investigated how the structure of the urban landscape, food resources, potential competitors, predators, and interaction between these factors influence the abundance of house sparrow Passer domesticus and the tree sparrow P. montanus in sixty 25 ha plots distributed randomly across residential areas of the city of Pozna5 （Poland）. The abundance of the house sparrow was positively correlated with the abundance of pigeons but negatively correlated with human-related food resources. There were significant interaction terms between abundances of other urban species and habitat variables in statistical models. For example, the abundance of house sparrow was negatively correlated with the abundance of corvids and tree sparrows but only when food resources were low. The abundance of tree sparrows positively correlated with density of streets and the distance from the city center. The abundance of this species positively correlated with the abundance of corvids when food resources were low but negatively correlated at low covers of green area. Our study indicates that associations between food resources, habitat covers, and the relative abundance of two sparrow species are altered by the abundance of other urban species. Competition, niche separation and social facilitation may be responsible for these interactive effects. Thus, biotic interactions should be included not only as an additive effect but also as an interaction term between abundance and habitat variables in statistical models predicting species abundance and occu rrence.

关键词： landscape ecology public information spatial autocorrelatien urban ecosystems.

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Cloning and biological comparison of Restin,a novel member of Mage superfamily

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Science China(Life Sciences) 2002年第4期45卷 412-420页

作者：赵忠良杨辉路凡柴玉波朱峰陈苏民 department of Biochemistry and molecular Biology The Fourth Military Medical University Xi'an 710032 China

In the present study, a new member of melanoma associated antigens (Mage), named Restin (219 amino acids), was identified from HL-60 cell induced by all-trans-retinoic acid (ATRA) by PCR-based subtractive hybridization. Bioinformatics analysis found this novel gene shares high homolog with Necdin (a neuronal growth suppressor, 49%). Both of them are basic proteins. Moreover, the Restin, Necdin and Mages are in one protein superfamily. This fact indicates that the Restin and Mages are mutually related but functionally different. Further analysis found that they can be divided into two subgroups, the acid and the basic. Restin, Necdin and Mage-D1 have an alkaline conserve region (PI is from 8.6 to 10.1), which are not or less expressed in tumor tissues but mostly in normal tissues. It has been reported that Necdin can arrest the cell proliferation by interaction with p53 and E2F1. Therefore, all of them are probably related to arrest the cell cycle. However, the Mage A and C are primarily acid proteins (PI is from 4.2 to 4.9), not expressed in normal tissues but in tumors. It is quite probable that these proteins are involved in the cell proliferation. We therefore suggest that these two protein families might be a pair of control elements of cell cycle-l'in cycle or out of cycle'.

关键词： Bioinformatics, Mage, Necdin, Restin.

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Evaluation of anti-metastatic effect of chitosan nanoparticles on esophageal cancer-associated fibroblasts

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Journal of Cancer Metastasis and Treatment 2016年第1期2卷 259-267页

作者： Pravin D.Potdar Aashutosh U.Shetti department of molecular Medicine and Biology Jaslok Hospital and Research CentreMumbai 400026India

Aim:Esophageal cancer is one of the major types of cancers,causing death of approximately 5%of all cancer *** is due,in large part,to both relatively ineffectual and unavailable *** order to develop an effective treatment strategy against esophageal cancer,it is important to target metastatic *** the present study,we have used a cancer-associated fibroblast(CAF)cell line derived from culturing peripheral blood mononuclear cells from a metastatic esophageal cancer patient to see whether chitosan nanoparticles(Ch-Np)treatment can modulate the metastatic phenotype of CAF cells by using various cellular and molecular ***:A CAF cell line was developed from peripheral blood mononuclear cells(PBMC)from a metastatic esophageal cancer *** cells were treated with 100μg/mL of chitosan nanoparticle in vitro for the morphological and oncogenic characteristic studies,along with the expression of various genes involved in process of tumor development and *** such as Light and Phase Contrast Microscopy,cell growth rate,Scratch metastatic assay,and molecular profiling were carried out to see changes in CAF cells before and after Ch-Np ***:It was observed that CAF cells grew in monolayer and had a doubling time of 25±0.38 ***,the cells had a fibroblastic *** treatment with 100μg/mL of Ch-Np in vitro,there was an increased doubling time to 30±0.83 ***,Scratch Assay showed an inhibition in the metastatic property of these *** findings were confirmed with gene expression *** was also observed that there was complete down-regulation of metastatic genes MMP1 and MMP9 and chemokines such as CXCR-4,CXCR-7,CCR-5,and SDF-1,indicating that Ch-Np inhibited the metastatic characteristic of CAF ***:This study has shown that there was an inhibition of metastatic properties of CAF cells after treatment with Ch-Np,suggesting that Ch-Np can be a delivery system used

关键词： Cancer-associated fibroblast molecular markers metastasis chitosan nanoparticle anti-metastatic metastatic genes

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Myeloid peroxisome proliferator-activated receptor α deficiency accelerates liver regeneration via IL-6/STAT3 pathway after 2/3 partial hepatectomy in mice

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Hepatobiliary Surgery and Nutrition 2022年第2期11卷 199-211,I0007页

作者： Guomin Xie Yanting Song Na Li Zhenzhen Zhang Xia Wang Ye Liu Shiyu Jiao Ming Wei Baoqi Yu Yan Wang Hua Wang Aijuan Qu department of physiology and Pathophysiology School of Basic Medical SciencesCapital Medical UniversityBeijingChina Key Laboratory of Remodeling-Related Cardiovascular Diseases Ministry of EducationBeijingChina department of Endocrinology Beijing Chaoyang Hospital Affiliated to Capital Medical UniversityBeijingChina department of Infectious Diseases Peking University First HospitalBeijingChina department of Oncology the First Affiliated Hospital of Anhui Medical UniversityHefeiChina

Background:Liver regeneration is a fundamental process for sustained body homeostasis and liver function recovery after *** evidence demonstrates that myeloid cells play a critical role in liver regeneration by secreting cytokines and growth *** proliferator-activated receptorα(PPARα),the target of clinical lipid-lowering fibrate drugs,regulates cell metabolism,proliferation,and ***,the role of myeloid PPARαin partial hepatectomy(PHx)-induced liver regeneration remains ***:Myeloid-specific PPARa-deficient(Ppara^(Mye−/−))mice and the littermate controls(Ppara^(fl/fl))were subjected to sham or 2/3 PHx to induce liver *** proliferation and mitosis were assessed by immunohistochemical(IHC)staining for 5-bromo-2'-deoxyuridine(BrdU)and Ki67 as well as hematoxylin and eosin(H&E)*** and neutrophil infiltration into livers were reflected by IHC staining for galectin-3 and myeloperoxidase(MPO)as well as flow cytometry *** migration ability was evaluated by transwell *** mRNA levels for cell cycle or inflammation-related genes were measured by quantitative real-time RT-PCR(qPCR).The protein levels of cell proliferation related protein and phosphorylated signal transducer and activator of transcription 3(STAT3)were detected by Western ***:Ppara^(Mye−/−)mice showed enhanced hepatocyte proliferation and mitosis at 32 h after PHx compared with Ppara^(fl/fl)mice,which was consistent with increased proliferating cell nuclear antigen(Pcna)mRNA and cyclinD1(CYCD1)protein levels in Ppara^(Mye−/−)mice at 32 h after PHx,indicating an accelerated liver regeneration in Ppara^(Mye−/−)*** staining showed that macrophages and neutrophils were increased in Ppara^(Mye−/−)liver at 32 h after *** of Ppara^(Mye−/−)mice also showed an enhanced infiltration of M1 macrophages at 32 h after *** vitro,Ppara-deficient bone marrow-derived macrophages(BMDMs)exhibited ma

关键词： Liver regeneration peroxisome proliferator-activated receptorα(PPARα) myeloid cell signal transducer and activator of transcription 3(STAT3) interleukin 6(IL-6)

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Targeting the P2X7 receptor in microglial cells to prevent brain inflammation

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Neural Regeneration Research 2020年第7期15卷 1245-1246页

作者： Lin-Hua Jiang Sébastien Roger School of Biomedical Sciences Faculty of Biological SciencesUniversity of LeedsSino-UK Joint Laboratory of Brain Function and Injury and Department of Physiology and NeurobiologyXinxiang Medical UniversityXinxiangHenan ProvinceChina EA4245 TransplantationImmunology and InflammationFaculty of MedicineUniversity of ToursToursFrance

Microglial cells are the key innate immune cells in the brain and they are crucial in maintaining brain parenchyma *** physiological conditions,microglial cells assume a ramified morphology with a small cell body and an extensive network of fine processes,which secrete neurotrophic factors and patrol the surroundings in search for pathogens and eliminate cellular debris via *** cells express a repertoire of pattern recognition receptors(PRRs)that enable them to detect diverse danger-associated molecular patterns(DAMPs)released from damaged cells or cells under stress,or pathogen-associated molecular patterns generated by pathogens during infection.

关键词： IL ATP APP Targeting the P2X7 receptor in microglial cells to prevent brain inflammation

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Default polyfunctional T helper 1 response to ample signal 1 alone

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cellular & molecular Immunology 2021年第7期18卷 1809-1822页

作者： Luca Danelli Georgina Cornish Julia Merkenschlager George Kassiotis Present address:Laboratory of molecular Immunology The Rockefeller UniversityNew YorkNY10065USA department of Medicine Faculty of MedicineImperial College LondonLondonW21PGUK Retroviral Immunology The Francis Crick Institute1 Midland RoadLondonNW11ATUK

CD4^(+)T cells integrate well-defined signals from the T-cell receptor(TCR)(signal 1)and a host of costimulatory molecules(signal 2)to initiate clonal expansion and differentiation into diverse functional T helper(Th)***,our ability to guide the expansion of context-appropriate Th subsets by deploying these signals in vaccination remains *** cell-based vaccines,we selectively amplified signal 1 by exclusive presentation of an optimized peptide:MHC II(pMHC II)complex in the absence of classic *** to expectations,amplified signal 1 alone was strongly immunogenic and selectively expanded highaffinity TCR clonotypes,despite delivering intense TCR *** contrast to natural infection or standard vaccines,amplified signal 1,presented by a variety of professional and nonprofessional antigen-presenting cells(APCs),induced exclusively polyfunctional Th1 effector and memory cells,which protected against retroviral infection and tumor challenge,and expanded tumor-reactive CD4^(+)T cells otherwise rendered unresponsive in tumor-bearing ***,our findings uncover a default Th1 response to ample signal 1 and offer a means to selectively prime such protective responses by vaccination.

关键词： CD4 T cell priming T helper differentiation peptide-MHC II complex

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