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Inositol-requiring enzyme 1α is required for gut development in Xenopus lavies embryos

World Journal of Gastroenterology 2013年第2期19卷 227-234页

作者： Jing Guo Xin-Xin Li Jiao-Jiao Feng Chen-Yang Yin XueJun Wang Ning Wang Li Yuan department of biochemistry and molecular Biology Nanjing Medical University

AIM:To investigate the role of inositol-requiring enzyme 1α(IRE1α) in gut development of Xenopus lavies ***:Xenopus embryos were obtained with in vitro fertilization and cultured in 0.1 × *** and half nanogram of IRE1α,1 ng of IRE1α-GR mRNA,1 ng of IRE1αΔC-GR mRNA,and 50 ng of IRE1α morpholino oligonucleotide(MO) or XBP1(C)MO were injected into four blastomeres at 4-cell stage for scoring the phenotype and marker gene *** rescue the effect of IRE1α MO,1 ng of IRE1α-GR mRNA was coinjected with 50 ng of *** the activation of the GR-fusion proteins,dexamethasone was prepared as 5 mmol/L stock solutions in 100% ethanol and applied to the mRNA injected embryos at desired stages in a concentration of 10 μmol/L in 0.1 × *** were kept in dexamethasone up to stage ***-mount in situ hybridization was used to determine specific gene expression,such as IRE1α,IRE1β,Xbra and Xsox17α.IRE1α protein expression during Xenopus embryogenesis was detected by Western ***:In the whole-mount in situ hybridization analysis,xenopus IRE1α and IRE1β showed quite different expression pattern during tadpole *** relatively higher expression of IRE1α was observed in the pancreas,and significant transcription of IRE1β was found in the ***1α protein could be detected at all developmental stages analyzed,from stage 1 to stage ***-of-function assay showed that IRE1α mRNA injected embryos at tailbud stage were nearly normal and the expression of the pan-mesodermal marker gene Xbra and the endodermal gene Xsox17α at stage 10.5 was not significantly changed in embryos injected with IRE1α mRNA as compared to uninjected control *** at tadpole stage,the embryos injected with IRE1α-GR mRNA did not display overt phenotype,such as gut-coiling ***-of-function assay demonstrated that the IRE1α MO injected embryos were morphologically normal before the tailbud *** did not observe a significant change of mesodermal and end

关键词： Inositol-requiring enzyme 1α XBP1 Xenopus lavies Gut Development

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EXPRESSION OF N-ras GENE IN HUMAN PRIMARY HEPATOCARCINOMA

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Science in China,Ser.B 1986年第2期29卷 181-186页

作者：胡利富王露萍居丽雯黄乐泓李晓林李秀珍顾健人 department of biochemistry and molecular Biology Shanghai Cancer Institute

Poly(A)~+ RNA was isolated from 9 specimens of human primary hepatic carcinoma, 1 non-tumorous liver tissue adjacent to cancer and 1 normal liver tissue samples. The Oligo-dT cellulose-purified poly(A)~+ RNAs were subjected to formaldehyde agarose gel electrophoresis, Northern transfer and hybridization with various ancogene probes. Two RNA species, 5.6 kb and 2.2 kb wore identified by N-ras gone hybridization in 6 out of 9 mRNA samples from primary hepatic carcinoma specimen. N-ras specific mRNA was not detectable in mRNA samples from normal human liver and tumor surrounding cirrhotic tissue. No detectable hybridization of mRNA from hepatoma and normal liver with Ki-ras or Ha-ras was *** human N-ras gene has been identified in DNA of mouse transfeetants transformed with PHC DNA, it strongly suggests that N-ras gene might be responsible for the transforming activity of part of cases of human liver cancer.

关键词： PHC EXPRESSION OF N-ras GENE IN HUMAN PRIMARY HEPATOCARCINOMA RNA HCI

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The structural biology of ryanodine receptors

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Science China(Life Sciences) 2011年第8期54卷 712-724页

作者： Lynn KIMLICKA Filip VAN PETEGEM University of British Columbia Department of Biochemistry and Molecular Biology

Ryanodine receptors are ion channels that allow for the release of Ca2+ from the endoplasmic or sarcoplasmic *** are expressed in many different cell types but are best known for their predominance in skeletal and cardiac myocytes,where they are directly involved in excitation-contraction *** molecular weights exceeding 2 MDa,Ryanodine Receptors are the largest ion channels known to date and present major challenges for structural *** their discovery in the 1980s,significant progress has been made in understanding their behaviour through multiple structural ***-electron microscopy reconstructions of intact channels depict a mushroom-shaped structure with a large cytoplasmic region that pre-sents many binding sites for regulatory *** region undergoes significant motions during opening and closing of the channel,demonstrating that the Ryanodine Receptor is a bona fide allosteric ***-resolution structures through X-ray crystallography and NMR currently cover～11% of the entire *** combination of high-and low-resolution methods allows us to build pseudo-atomic *** we present an overview of the electron microscopy,NMR,and crystallographic analyses of this membrane protein giant.

关键词： calcium release excitation-contraction coupling genetic disease structural biology calcium release channel ion channel

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IDENTIFICATION OF HUMAN N-ras AS THE COMMON ONCOGENE IN NIH 3T3 CELLS TRANSFORMED WITH DNAs FROM HUMAN PRIMARY HEPATIC CANCER AND HEPATOMA 7402 LINE

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Science China chemistry 1986年第2期29卷 173-180页

作者：顾健人田培坤万大方王翔李宏年潘志美黄乐泓李秀珍蒋惠秋 department of biochemistry and molecular Biology Shanghai Cancer Institute

DNA was extracted from NIH 3T3 cells transformed with DNAs from human primary hepatic cancer （PHC） and Hepatoma 7402 cell line. The transformant DNA was analyzed by Southern transfer and hybridization withP-labeled probes of various *** EcoRI 7.2 and 9.0 kb bands characteristic of human N-ras gene were identified in transformed NIH 3T3 cells derived both from PHC and 7402 DNA. The BamHI 6.6kb band characteristic of human c-Ha-ras I was present only in 7402 transformants, but not in PHC ***-methionine incorporation, immunoprecipitation with anti-p21 monoclonal antibodies, SDS-PAGE and autoradiography, it was demonstrated that p21 synthesis was remarkably enhanced in 1402 cells as well as in transformed cells derived from both 7402 and PHC *** the data together, it. strongly implies that N-ras is one of the transforming genes for human liver cancer.

关键词： DNA PHC IDENTIFICATION OF HUMAN N-ras AS THE COMMON ONCOGENE IN NIH 3T3 CELLS TRANSFORMED WITH DNAs FROM HUMAN PRIMARY HEPATIC CANCER AND HEPATOMA 7402 LINE NIH

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Correlation of microRNAs responding to high dose γ-irradiation with predicted target mRNAs in HeLa cells using microarray analyses

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Chinese Science Bulletin 2013年第36期58卷 4622-4629页

作者： HU Zheng TIE Yi Lü GuiXiang FU HanJiang XING RuiYun ZHU Jie SUN ZhiXian ZHENG XiaoFei Beijing Institute of Radiation Medicine department of biochemistry and molecular Biology Harbin Medical University

An increasing data indicates that altered microRNAs(miRNAs)participate in the radiation-induced DNA damage ***,a correlation of mRNA and miRNA profiles across the entire genome and in response to irradiation has not been thoroughly *** analyzed miRNA microarray data collected from HeLa cells after ionizing radiation(IR),quantified the expression profiles of mRNAs and performed comparative analysis of the data sets using target prediction algorithms,Gene Ontology(GO)analysis,pathway analysis,and gene network *** results showed that the altered miRNAs were involved in regulation of various cellular ***-gene network analyses revealed that miR-186,miR-106b,miR-15a/b,CCND1and CDK6 played vital role in the cellular radiation *** qRT-PCR,we confirmed that twenty-two miRNAs showed differential expression in HeLa cells treated with IR and some of these miRNAs affected cell cycle *** study demonstrated that miRNAs influence gene expression in the entire genome during the cellular radiation response and suggested vital pathways for further research.

关键词： HeLa细胞目标基因 microRNA 预测算法微阵列分析照射高剂量 miRNA

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Synthesis and Characterization of [(C_5H_4SiMe_2~tBu)_2Ln(μ-S^nBu)]_2 (Ln=Y,Er)

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Chinese Journal of Structural chemistry 2008年第5期27卷 523-528页

作者：李艳荣张正星陈珍霞刘瑞婷周锡庚 department of chemistry Molecular Catalysis and Innovative Material Laboratory Fudan University

（C5H4SiMe2^tBu）2Ln^nBu reacted with 1 equiv, of elemental sulfur in toluene at ambient temperature to yield the corresponding lanthanocene thiolates [（C5H4SiMe2^tBu）2Ln（μ-S^nBu）]2 （Ln = Y （1）, Er （2））. Complexes 1 and 2 have been characterized by elemental analysis, IR, mass spectroscopy and X-ray single-crystal diffraction analysis. Both complexes are of monoclinic with space group P21/c, formula C52H94S2Si4Y2 1 （C52H94S2Si4Er2 2） Mr = 1073.57 （1230.27）, a = 8.495（2）（8.41（2））, b = 26.913（8）（26.67（7））, c = 13.756（4）（13.68（4）） A, α = 90（90）, β = 101.184（5）（101.57（4））, γ = 90 （90）°, V= 3085.1（15）（3007（14）） A^3, Dc = 1.156 （1.359） g·cm^-3, Z= 2 （2）, F（000） = 1144 （1260）, μ = 2.046 （2.951） cm^-1, R = 0.0687 （0.0749） and wR = 0.1306 （0.1507） for observed reflections with I 〉 2σ（I）. X-ray structures of 1 and 2 definitively prove that only one sulfur atom is inserted into the Ln-C（^nBu） bond, forming a thiolate ligand.

关键词： organolanthanide crystal structure insertion elemental sulfur thiolate

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Thermochemical Study on Ternary Solid Complex of La(Gly)_2(Ala)_3Cl_3·2H_2O(s)

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Journal of Rare Earths 2007年第3期25卷 373-376页

作者：叶丽娟李强国李旭杨德俊刘义 department of chemistry and Life Science Xiangnan University College of chemistry and molecular Science Wuhan University

The complex of lanthanum chloride with Glycine and Alanine,La（Gly）2（Ala）3Cl3·2H2O,was synthesized and characterized by IR,elementary analysis,thermogravimetric analysis,and chemical *** dissolution enthalpies of LaCl3 ·7H2O（s）,2Gly（s）＋ 3Ala（s）and La（Gly）2（Ala）3Cl3 ·2H2O（s）were determined in 2 mol·L-1 HCl by a solution-reaction isoperibol *** designing a thermochemical cycle in terms of Hess＇ Law and through calculation,the reaction enthalpy of lanthanum chloride seven-hydrate with Glycine and Alanine was obtained：ΔrHθm（298.15 K）=（29.652±0.504）kJ·mol-1,and the standard enthalpy of formation of La（Gly）2（Ala）3Cl3·2H2O（s）ΔfHθm [La（Gly）2（Ala）3Cl3·2H2O,s,298.15 K]=-4467.6±8.3 kJ·mol-1.

关键词： lanthanum complex Glycine Alanine thermochemistry standard molar enthalpy of formation rare earths

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Effects of P301L-TAU on post-translational modifications of microtubules in human iPSC-derived cortical neurons and TAU transgenic mice

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Neural Regeneration Research 2025年第8期20卷 2348-2360页

作者： Mohamed Aghyad Al Kabbani Christoph Köhler Hans Zempel Institute of Human Genetics Faculty of Medicine and University Hospital CologneUniversity of CologneCologneGermany Center for molecular Medicine Cologne(CMMC) University of CologneCologneGermany Center Anatomy Department ⅡMedical FacultyUniversity of CologneCologneGermany

TAU is a microtubule-associated protein that promotes microtubule assembly and stability in the *** is missorted and aggregated in an array of diseases known as *** are essential for neuronal function and regulated via a complex set of post-translational modifications,changes of which affect microtubule stability and dynamics,microtubule interaction with other proteins and cellular structures,and mediate recruitment of microtubule-severing *** impairment of microtubule dynamics causes neuronal dysfunction,we hypothesize cognitive impairment in human disease to be impacted by impairment of microtubule *** therefore aimed to study the effects of a disease-causing mutation of TAU(P301L)on the levels and localization of microtubule post-translational modifications indicative of microtubule stability and dynamics,to assess whether P301L-TAU causes stability-changing modifications to *** investigate TAU localization,phosphorylation,and effects on tubulin post-translational modifications,we expressed wild-type or P301L-TAU in human MAPT-KO induced pluripotent stem cell-derived neurons(i Neurons)and studied TAU in neurons in the hippocampus of mice transgenic for human P301L-TAU(p R5 mice).Human neurons expressing the longest TAU isoform(2N4R)with the P301L mutation showed increased TAU phosphorylation at the AT8,but not the p-Ser-262 epitope,and increased polyglutamylation and acetylation of microtubules compared with endogenous TAU-expressing neurons.P301L-TAU showed pronounced somatodendritic presence,but also successful axonal enrichment and a similar axodendritic distribution comparable to exogenously expressed 2N4R-wildtype-TAU.P301L-TAU-expressing hippocampal neurons in transgenic mice showed prominent missorting and tauopathy-typical AT8-phosphorylation of TAU and increased polyglutamylation,but reduced acetylation,of microtubules compared with non-transgenic *** sum,P301L-TAU results in changes in micr

关键词： human induced pluripotent stem cell microtubules P301L pR5 mice TAU tauopathy tubulin code

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Increasing polymer scission rate by tying an overhand knot

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Science China chemistry 2024年第11期67卷 3498-3499页

作者： Yuheng Hu Jennifer T.Ou Jim YHuang Yuanning Feng department of chemistry and biochemistry The University of OklahomaNormanOK 73019USA

Similar to the phenomenon of knotted ropes in life, knotted climbing ropes will reduce their strength due to knots. Although computer simulations [1], theoretical speculation[2,3], and intuition [3] suggest that similar processes may occur at the nanoscale, this hypothesis has not been experimentally proven before. With the increasing maturity of molecular recognition and self-assembly, a research communication by Leigh et al. [4] published in Nature chemistry introduced a molecular overhand knot into a polymer to study its effect on the scission rate of the polymer chain under tension.

关键词： polymer hypothesis hough

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Time course and mechanism of brain oxidative stress and damage for redox active and inactive transition metals overload

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BIOCELL 2016年第1期40卷 19-21页

作者： NIDIA FERRAROTTI ROSARIO MUSACCO-SEBIO CHRISTIAN SAPORITO-MAGRINA JUAN MANUEL ACOSTA MARISA REPETTO Clinical biochemistry department Faculty of Pharmacy and BiochemistryInstitute of Biochemistry and Molecular Medicine(IBIMOL)University of Buenos Aires-CONICETBuenos AiresArgentina General and Inorganic chemistry Faculty of Pharmacy and BiochemistryInstitute of Biochemistry and Molecular Medicine(IBIMOL)University of Buenos Aires-CONICETBuenos AiresArgentina.

The objective of this work was to study the in vivo time course of biochemical processes of oxidative damage in the brain of Sprague-Dawley rats that received an acute overload of the redox active metals iron(Fe)and copper(Cu),and the redox inactive cobalt(Co)and nickel(Ni).Oxidative stress indicators(phospholipid and protein oxidation),glutathione(GSH),antioxidant enzymes and NADPH oxidase activities,and the plasma inflam-matory cytokine(IL-6)were *** results showed that in brain oxidative mechanisms for both sets of metal are different,however in both cases are *** mechanism for Fe and Cu oxidative damage is mediated by the generation of the free radical hydroxyl(Fenton reaction and homolytic cleavage of hydroperoxides).Two events of antioxidant protection prior to oxidation of phospholipids and proteins by Fe and Cu are *** first process is the use of GSH and the second is the increased activity of the Cu,Zn-SOD and catalase *** oxidative mechanism for metal redox inactive is the consumption of GSH,NADPH oxidase activation and inflammatory response mediated by *** increased protein oxidation as a result of the inflammatory *** produced increments of phospholipid oxidation and SOD activity.

关键词： metals oxidative damage oxidative stress brain lipid peroxidation

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