检索结果-南通市图书馆

iPSC-derived cranial neural crest-like cells can replicate dental pulp tissue with the aid of angiogenic hydrogel

Bioactive Materials 2022年第8期7卷 290-301页

作者： Yoshifumi Kobayashi Julie Nouet Erdenechimeg Baljinnyam Zain Siddiqui Daniel HFine Diego Fraidenraich Vivek A.Kumar Emi Shimizu department of oral biology Rutgers School of Dental MedicineNewarkNJ07103USA department of Cell biology and Molecular Medicine Rutgers New Jersey Medical SchoolNewarkNJ07103USA department of biomedical engineering New Jersey Institute of TechnologyNewarkNJ07102USA

The dental pulp has irreplaceable roles in maintaining healthy teeth and its regeneration is a primary aim of regenerative endodontics.This study aimed to replicate the characteristics of dental pulp tissue by using cranial neural crest(CNC)-like cells(CNCLCs);these cells were generated by modifying several steps of a previously established method for deriving NC-like cells from induced pluripotent stem cells(iPSCs).CNC is the anterior region of the neural crest in vertebrate embryos,which contains the primordium of dental pulp cells or odontoblasts.The produced CNCLCs showed approximately 2.5-12,000-fold upregulations of major CNC marker genes.Furthermore,the CNCLCs exhibited remarkable odontoblastic differentiation ability,especially when treated with a combination of the fibroblast growth factors(FGFs)FGF4 and FGF9.The FGFs induced odontoblast marker genes by 1.7-5.0-fold,as compared to bone morphogenetic protein 4(BMP4)treatment.In a mouse subcutaneous implant model,the CNCLCs briefly fated with FGF4+FGF9 replicated dental pulp tissue characteristics,such as harboring odontoblast-like cells,a dentin-like layer,and vast neovascularization,induced by the angiogenic self-assembling peptide hydrogel(SAPH),SLan.SLan acts as a versatile biocompatible scaffold in the canal space.This study demonstrated a successful collaboration between regenerative medicine and SAPH technology.

关键词： Regenerative endodontics iPSC Cranial neural crest Fibroblast growth factor Odontoblastic differentiation Angiogenic self-assembling peptide hydrogel

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

mTORC1 signaling pathway regulates tooth repair

引用

International Journal of oral Science 2023年第1期15卷 172-183页

作者： Honghong Liu Yu Yue Zhiyun Xu Li Guo Chuan Wu Da Zhang Lingfei Luo Wenming Huang Hong Chen Deqin Yang department of Endodontics Stomatological Hospital of Chongqing Medical UniversityChongqingChina Stomatological Hospital of Chongqing Medical University ChongqingChina Chongqing Key Laboratory of oral Diseases and biomedical Sciences ChongqingChina Chongqing Municipal Key Laboratory of oral biomedical engineering of Higher Education ChongqingChina institute of Developmental biology and Regenerative Medicine Southwest UniversityChongqingChina

Tooth germ injury can lead to abnormal tooth development and even tooth loss,affecting various aspects of the stomatognathic system including form,function,and appearance.However,the research about tooth germ injury model on cellular and molecule mechanism of tooth germ repair is still very limited.Therefore,it is of great importance for the prevention and treatment of tooth germ injury to study the important mechanism of tooth germ repair by a tooth germ injury model.Here,we constructed a Tg(dlx2b:Dendra2-NTR)transgenic line that labeled tooth germ specifically.Taking advantage of the NTR/Mtz system,the dlx2b+tooth germ cells were depleted by Mtz effectively.The process of tooth germ repair was evaluated by antibody staining,in situ hybridization,Ed U staining and alizarin red staining.The severely injured tooth germ was repaired in several days after Mtz treatment was stopped.In the early stage of tooth germ repair,the expression of phosphorylated 4E-BP1 was increased,indicating that mTORC1 is activated.Inhibition of mTORC1 signaling in vitro or knockdown of mTORC1 signaling in vivo could inhibit the repair of injured tooth germ.Normally,mouse incisors were repaired after damage,but inhibition/promotion of mTORC1 signaling inhibited/promoted this repair progress.Overall,we are the first to construct a stable and repeatable repair model of severe tooth germ injury,and our results reveal that mTORC1 signaling plays a crucial role during tooth germ repair,providing a potential target for clinical treatment of tooth germ injury.

关键词： prevention inhibited mTORC1

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Injectable hybrid inorganic nanoscaffold as rapid stem cell assembly template for cartilage repair

引用

National Science Review 2022年第4期9卷 116-127页

作者： Shenqiang Wang Letao Yang Bolei Cai Fuwei Liu Yannan Hou Hua Zheng Fang Cheng Hepeng Zhang Le Wang Xiaoyi Wang Qianxin Lv Liang Kong Ki-Bum Lee Qiuyu Zhang Key Laboratory of Special Functional and Smart Polymer Materials of Ministry of Industry and Information Technology School of Chemistry and Chemical EngineeringNorthwestern Polytechnical University Xi'an Key Laboratory of Functional Organic Porous Materials School of Chemistry and Chemical EngineeringNorthwestern Polytechnical University department of Chemistry and Chemical biology Rutgers University department of biomedical engineering Columbia University State Key Laboratory of Military Stomatology & National Clinical Research Center for oral Diseases & Shaanxi Key Laboratory of oral Diseases Department of Oral and Maxillofacial SurgerySchool of StomatologyThe Fourth Military Medical University Research & Development institute of Northwestern Polytechnical University in Shenzhen

Cartilage injuries are o?ten devastating and most cannot be cured because of the intrinsically low regenerative capacity of cartilage tissues. Although stem-cell therapy has shown enormous potential for cartilage repair, the therapeutic outcome has been restricted by low survival rates and poor chondrocyte differentiation in vivo. Here, we report an injectable hybrid inorganic(IHI) nanoscaffold that facilitates fast assembly, enhances survival and regulates chondrogenic differentiation of stem cells. IHI nanoscaffolds that strongly bind to extracellular matrix(ECM) proteins assemble stem cells through synergistic 3 D cell–cell and cell–matrix interactions, creating a favorable physical microenvironment for stem-cell survival and differentiation in vitro and in vivo. Additionally, chondrogenic factors can be loaded into nanoscaffolds with a high capacity, which allows deep, homogenous drug delivery into assembled 3 D stem-cell-derived tissues for effective control over the soluble microenvironment of stem cells. The developed IHI nanoscaffolds that assemble with stem cells are injectable. They also scavenge reactive oxygen species and timely biodegrade for proper integration into injured cartilage tissues. Implantation of stem-cell-assembled IHI nanoscaffolds into injured cartilage results in accelerated tissue regeneration and functional recovery. By establishing our IHI nanoscaffold-templated 3 D stem-cell assembly method, we provide a promising approach to better overcoming the inhibitory microenvironment associated with cartilage injuries and to advance current stem-cell-based tissue engineering.

关键词： injectable nanoscaffold 3D cell culture tissue engineering cartilage repair stem-cell therapy

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Are human dental papilla-derived stem cell and human brain-derived neural stem cell transplantations suitable for treatment of Parkinson’s disease?

引用

Neural Regeneration Research 2013年第13期8卷 1190-1200页

作者： Hyung Ho Yoon Joongkee Min Nari Shin Yong Hwan Kim Jin-Mo Kim Yu-Shik Hwang Jun-Kyo Francis Suh Onyou Hwang Sang Ryong Jeon department of Neurological Surgery Asan Medical Center University of Ulsan College of Medicine department of Biochemistry and Molecular biology University of Ulsan College of Medicine department of Computer Science and engineering University of Notre Dame Notre Dame Center for Bionics of Korea institute of Science and Technology department of maxillofacial biomedical engineering Institute of Oral Biology School of Dentistry Kyung Hee University

Transplantation of neural stem cells has been reported as a possible approach for replacing impaired dopaminergic neurons. In this study, we tested the efficacy of early-stage human dental papilla-derived stem cells and human brain-derived neural stem cells in rat models of 6-hydroxydopamine-induced Parkinson＇s disease. Rats received a unilateral injection of 6-hydroxydopamine into right medial forebrain bundle, followed 3 weeks later by injections of PBS, early-stage human dental papilla-derived stem cells, or human brain-derived neural stem cells into the ipsilateral striatum. All of the rats in the human dental papilla-derived stem cell group died from tumor formation at around 2 weeks following cell transplantation. Postmortem examinations revealed homogeneous malignant tumors in the striatum of the human dental papilla-derived stem cell group. Stepping tests revealed that human brain-derived neural stem cell transplantation did not improve motor dysfunction. In apomorphine-induced rotation tests, neither the human brain-derived neural stem cell group nor the control groups （PBS injection） demonstrated significant changes. Glucose metabolism in the lesioned side of striatum was reduced by human brain-derived neural stem cell transplantation. [18F]-FP-CIT PET scans in the striatum did not demonstrate a significant increase in the human brain-derived neural stem cell group. Tyrosine hydroxylase （dopaminergic neuronal marker） staining and G protein-activated inward rectifier potassium channel 2 （A9 dopaminergic neuronal marker） were positive in the lesioned side of striatum in the human brain-derived neural stem cell group. The use of early-stage human dental papilla-derived stern cells confirmed its tendency to form tumors. Human brain-derived neural stem cells could be partially differentiated into dopaminergic neurons, but they did not secrete dopamine.

关键词： neural regeneration stern cells cell transplantation glucose metabolism human brain-derivedneural stem cells human dental papilla-derived stem cells Parkinson＇s disease positron emissiontomography grants-supported paper neuroregeneration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Viral inactivation by light

引用

eLight 2022年第1期2卷 237-254页

作者： Mohammad Sadraeian Le Zhang Farzaneh Aavani Esmaeil Biazar Dayong Jin Present Address:institute for biomedical Materials and Devices(IBMD) Faculty of ScienceUniversity of Technology SydneySydneyNSW 2007Australia department of oral and maxillofacial Surgery Division of Regenerative Orofacial MedicineUniversity Hospital Hamburg-Eppendorf20251 HamburgGermany department of biomedical engineering Islamic Azad UniversityTonekabon BranchTonekabonIran UTS‑SUStech Joint Research Centre for biomedical Materials&Devices Department of Biomedical EngineeringCollege of EngineeringSouthern University of Science and TechnologyShenzhenGuangdongChina

Nowadays,viral infections are one of the greatest challenges for medical sciences and human society.While antiviral compounds and chemical inactivation remain inadequate,physical approaches based on irradiation provide new potentials for prevention and treatment of viral infections,without the risk of drug resistance and other unwanted side effects.Light across the electromagnetic spectrum can inactivate the virions using ionizing and non-ionizing radiations.This review highlights the anti-viral utility of radiant methods from the aspects of ionizing radiation,including high energy ultraviolet,gamma ray,X-ray,and neutron,and non-ionizing photo-inactivation,including lasers and blue light.

关键词： Antiviral therapy Enveloped virus Photo-inactivation Ionizing radiation Nuclear radiations

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cells and material-based strategies for regenerative endodontics

引用

Bioactive Materials 2022年第8期7卷 234-249页

作者： Zain Siddiqui Amanda M.Acevedo-Jake Alexandra Griffith Nurten Kadincesme Kinga Dabek Dana Hindi Ka Kyung Kim Yoshifumi Kobayashi Emi Shimizu Vivek Kumar department of biomedical engineering New Jersey Institute of TechnologyNewarkNJ07102USA department of oral biology Rutgers School of Dental MedicineNewarkNJ07103USA department of Endodontics Rutgers School of Dental MedicineNewarkNJ07103USA department of Chemicals and Materials engineering New Jersey Institute of TechnologyNewarkNJ07102USA department of biology New Jersey Institute of TechnologyNewarkNJ07102USA

The carious process leads to inflammation of pulp tissue.Current care options include root canal treatment or apexification.These procedures,however,result in the loss of tooth vitality,sensitivity,and healing.Pulp capping and dental pulp regeneration are continually evolving techniques to regenerate pulp tissue,avoiding necrosis and loss of vitality.Many studies have successfully employed stem/progenitor cell populations,revascularization approaches,scaffolds or material-based strategies for pulp regeneration.Here we outline advantages and disadvantages of different methods and techniques which are currently being used in the field of regenerative endodontics.We also summarize recent findings on efficacious peptide-based materials which target the dental niche..

关键词： Regenerative endodontics Pulp regeneration Tissue engineering Stem cells Scaffolds

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Liquiritigenin promotes osteogenic differentiation and prevents bone loss via inducing auto-lysosomal degradation and inhibiting apoptosis

引用

Genes & Diseases 2023年第1期10卷 284-300页

作者： Yu Qiu Yueyang Zhao Zhimin Long Aijia Song Peng Huang Kejian Wang Ling Xu David Paul Molloy Guiqiong He College of Stomatology Chongqing Medical UniversityChongqing Key Laboratory of Oral Diseases and Biomedical SciencesChongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher EducationChongqing400016China Chongqing Key Laboratory of Neurobiology Chongqing Medical UniversityChongqing 400016China department of Anatomy Chongqing Medical UniversityChongqing 400016China Laboratory of Medical Experiment Technology Institute of Life ScienceChongqing Medical UniversityChongqing 400016China department of Biochemistry and Molecular biology Chongqing Medical UniversityChongqing 400016China

Osteoporosis(OP)is a debilitating skeletal abnormality involving bone remodeling and bone cell homeostasis characterized by decreased bone strength and high fracture risk.A novel therapeutic intervention for OP by manipulating cellular autophagy-apoptosis processes to promote skeletal homeostasis is presented.Protective effects of the naturally occurring plant extract Liquiritigenin(LG)were demonstrated in an ovariectomy(OvX)-OP mouse model and preosteoblast MC3T3-E1 cells.Micro-CT and histological staining assessments of skeletal phenotype were applied alongside detection of autophagy activity in osteocytes and MC3T3-E1 cells by transmission electron microscopy(TEM).The effects of LG on chloroquine(CQ)-and the apoptosis-inducing TS-treated osteogenic differentiations and status of lysosomes within MC3T3-E1 cells were analyzed by Neutral red,Alizarin red S and alkaline phosphatase(ALP)staining and Western blot assays.Treatment with LG prevented bone loss,increased osteogenic differentiation in vivo and in vitro,and inhibited osteoclast formation to some extent.TEM analyses revealed that LG can improve auto-lysosomal degradation within osteocytes from OVX mice and MC3T3-E1 cells.The abnormal status of lysosomes associated with CQ and TS treatments was notably alleviated by LG which also reduced levels of apoptosis-induced inhibition of osteogenic differentiation and averted abnormal osteogenic differentiation as a consequence of a blockage in autolysosome degradation.Overall,LG stimulates bone growth in Oovx mice through increased osteogenic differentiation and regulation of autophagyapoptosis mechanisms,presenting an auspicious natural therapy for Op.

关键词： Apoptosis Auto-lysosomal degradation Liquiritigenin Osteogenic differentiation Osteoporosis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Extracellular matrix remodelling in dental pulp tissue of carious human teeth through the prism of single-cell RNA sequencing

引用

International Journal of oral Science 2023年第3期15卷 450-461页

作者： Anamaria Balic Dilara Perver Pierfrancesco Pagella Hubert Rehrauer Bernd Stadlinger Andreas E.Moor Viola Vogel Thimios A.Mitsiadis Orofacial Development and Regeneration Institute of Oral BiologyCentre of Dental MedicineUniversity of ZurichZurichSwitzerland department of Health Sciences and Technology Institute of Translational MedicineETH ZurichZurichSwitzerland Functional Genomics Center Zurich ETH Zurich and University of ZurichZurichSwitzerland Clinic of Cranio-maxillofacial and oral Surgery University of ZurichZurichSwitzerland department of Biosystems Science and engineering ETH ZurichBaselSwitzerland

Carious lesions are bacteria-caused destructions of the mineralised dental tissues,marked by the simultaneous activation of immune responses and regenerative events within the soft dental pulp tissue.While major molecular players in tooth decay have been uncovered during the past years,a detailed map of the molecular and cellular landscape of the diseased pulp is still missing.In this study we used single-cell RNA sequencing analysis,supplemented with immunostaining,to generate a comprehensive single-cell atlas of the pulp of carious human teeth.Our data demonstrated modifications in the various cell clusters within the pulp of carious teeth,such as immune cells,mesenchymal stem cells(MSC)and fibroblasts,when compared to the pulp of healthy human teeth.Active immune response in the carious pulp tissue is accompanied by specific changes in the fibroblast and MSC clusters.These changes include the upregulation of genes encoding extracellular matrix(ECM)components,including COL1A1 and Fibronectin(FN1),and the enrichment of the fibroblast cluster with myofibroblasts.The incremental changes in the ECM composition of carious pulp tissues were further confirmed by immunostaining analyses.Assessment of the Fibronectin fibres under mechanical strain conditions showed a significant tension reduction in carious pulp tissues,compared to the healthy ones.The present data demonstrate molecular,cellular and biomechanical alterations in the pulp of human carious teeth,indicative of extensive ECM remodelling,reminiscent of fibrosis observed in other organs.This comprehensive atlas of carious human teeth can facilitate future studies of dental pathologies and enable comparative analyses across diseased organs.

关键词： organs pulp matrix

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

An automatic optimization method for minimizing supporting structures in additive manufacturing

引用

Advances in Manufacturing 2020年第1期8卷 49-58页

作者： Xiao-Jun Chen Jun-Lei Hu Qing-Long Zhou Constantinus Politis Yi Sun institute of biomedical Manufacturing and Life Quality engineering State Key Laboratory of Mechanical System and VibrationSchool of Mechanical EngineeringShanghai Jiao Tong UniversityShanghai 200240P.R.China OMFS-IMPATH Research Group Department of Imaging and PathologyFaculty of MedicineKatholieke Universiteit LeuvenLeuvenBelgium department of oral and maxillofacial Surgery University Hospitals LeuvenLeuvenBelgium

The amount of supporting structure usage has been a major research topic in layer-based additive manufacturing(AM)over the past years as it leads to increased fabrication time and decreased surface quality.Previous studies focused on deformation and topology optimization to eliminate the number of support structures.However,during the actual fabrication process,the properties of shape and topology are essential.Therefore,they should not be modified casually.In this study,we present an optimizer that reduces the number of supporting structures by identifying the prime printing direction.Without rotation,models are projected in each direction in space,and the basis units involved in the generation of support structures are separated.Furthermore,the area of the supporting structures is calculated.Eventually,the prime printing direction with minimal supporting area is obtained through pattern-searching method.The results of the experiment demonstrated that the printing area was reduced by up to 60%for some cases,and the surface quality was also improved correspondingly.Furthermore,both the material consumption and fabrication time were decreased in most cases.In future work,additional factors will be considered,such as the height of the supporting S Xiao-Jun Chen xiaojunchen@*** 1 institute of biomedical Manufacturing and Life Quality engineering,State Key Laboratory of Mechanical System and Vibration,School of Mechanical engineering,Shanghai Jiao Tong University,Shanghai 200240,P.R.China 2 OMFS-IMPATH Research Group,department of Imaging and Pathology,Faculty of Medicine,Katholieke Universiteit Leuven,Leuven,Belgium 3 department of oral and maxillofacial Surgery,University Hospitals Leuven,Leuven,Belgium structures and the adhesion locations to improve the efficiency of this optimizer.

关键词： Printing direction Optimization Supporting structures

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Degradable and Tunable Keratin-fibrinogen Hydrogel as Controlled Release System for Skin Tissue Regeneration

引用

Journal of Bionic engineering 2023年第3期20卷 1049-1059页

作者： Sung Jun Min Jae Seo Lee Haram Nah Ho-Jin Moon Sang Jin Lee Hyeon Jeong Kang Yu-Shik Hwang Il Keun Kwon Dong Nyoung Heo department of Dentistry Graduate SchoolKyung Hee University26 Kyungheedae-RoDongdaemun-GuSeoul02447Republic of Korea Biofriends Inc 26 Kyungheedae-RoDongdaemun-GuSeoul02447Republic of Korea department of Dental Materials School of DentistryKyung Hee University26 Kyungheedae-RoDongdaemun-GuSeoul02447Republic of Korea Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology School of DentistryKyung Hee UniversitySeoul02447Korea Kyung Hee University Medical Science Research institute Kyung Hee University23 Kyungheedae-RoDongdaemun-GuSeoul02447Republic of Korea

Biodegradable hydrogels are promising biomaterials for use in controlled-release systems for skin tissue regeneration. Controlled delivery systems constitute an important aspect of tissue engineering because they can modulate various physiological responses, including early immune response, tissue remodeling, and cell proliferation and maturation in the wound-healing process. Hydrogels composed of various biomaterials have been developed to overcome the limitations of conventional drug- or protein-delivery systems, such as limited targeting ability, low stability, and the induction of drug resistance. Hydrogels based on keratin, a natural polymer extracted from human hair, can provide adequate cell support and control homeostasis. Consequently, they can be applied for skin tissue engineering. In this study, we prepared degradable, tunable, and biocompatible hydrogels for controllable protein delivery. We synthesized keratin-fibrinogen (KER-FBG) by the chemical coupling reaction and prepared hydrogels through polymerization with thrombin. The structures and morphologies of the KER-FBG hydrogels were confirmed. Furthermore, the mechanical properties, swelling ratio, degradation, release behavior, and biocompatibility were investigated. The KER-FBG hydrogels presented promising biological performance, indicating that the material is suitable as a controlled protein delivery carrier.

关键词： Keratin Fibrinogen Hydrogel Drug delivery Degradable

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：