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Biodiversity of Free-Living marine Nematodes in the Southern Yellow Sea,China

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Journal of Ocean University of China 2016年第1期15卷 1-10页

作者： LIU Xiaoshou XU Man HUA Er ZHANG Zhinan College of marine life sciences Ocean University of China

Biodiversity pattems of free-living marine nematodes were studied using specific, taxonomic and phylogenetic diversity measures in the southern Yellow Sea, China. The results showed that the average of Shannon-Wiener diversity index （H＇） in the study area was 3.17. The higher values were distributed in the east part of Shandong coastal waters and north part of Jiangsu coastal waters, while the lower values were distributed in the southern Yellow Sea Cold Water Mass （YSCWM）. The average of taxonomic diversity （A） was 62.09 in the study region. The higher values were distributed in the transitional areas between the coastal areas and the southem YSCWM, while the lower values were distributed near the north part of Jiangsu coastal waters and the YSCWM. Results of correlation analysis of species diversity and taxonomic diversity showed that some of the two kinds of diversity index were inde- pendent, which suggested that combining the two kinds of diversity indices can reflect the ecological characteristics better. A test for 95% probability funnels of average taxonomic distinctness and variation in taxonomic distinctness suggested that Station 8794 （in the YSCWM） was outside of the 95% probability funnels, which may be due to the environmental stress. Results of correlation analysis between marine nematodes biodiversity and environmental variables showed that the sediment characteristics （Mdo and Silt-clay fraction） and phaeophorbide a （Pha-a） were the most important factors to determine the biodiversity patterns of marine nematodes.

关键词： species diversity taxonomic diversity flee-living marine nematode southem Yellow Sea

Pharmacokinetics and Biodegradation of Chitosan in Rats

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Journal of Ocean University of China 2015年第5期14卷 897-904页

作者： LI Hui JIANG Zhiwen HAN Baoqin NIU Shuyi DONG Wen LIU Wanshun College of marine life sciences Ocean University of China

Chitosan, an excellent biomedical material, has received a widespread in vivo application. In contrast, its metabolism and distribution once being implanted were less documented. In this study, the pharmacokinetics and biodegradation of fluorescein isothiocyanate(FITC) labeled and muscle implantation administrated chitosan in rats were investigated with fluorescence spectrophotometry, histological assay and gel chromatography. After implantation, chitosan was degraded gradually during its distribution to diverse organs. Among the tested organs, liver and kidney were found to be the first two highest in chitosan content, which was followed by heart, brain and spleen. Urinary excretion was believed to be the major pathway of chitosan elimination, yet 80% of chitosan administered to rats was not trackable in their urine. This indicated that the majority of chitosan was degraded in tissues. In average, the molecular weight of the degradation products of chitosan in diverse organs and urine was found to be <65 k Da. This further confirmed the in vivo degradation of chitosan. Our findings provided new evidences for the intensive and safe application of chitosan as a biomedical material.

关键词： chitosan fluorescein isothiocyanate pharmacokinetics biodegradation fluorescence spectrophotometry muscle implantation

Impact of Pollutant Concentration and Particle Deposition on the Radiative Flow of Casson-Micropolar Fluid between Parallel Plates

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Computer Modeling in Engineering & sciences 2025年第1期142卷 665-690页

作者： Ghaliah Alhamzi Badr Saad T.Alkahtani Ravi Shanker Dubey Vinutha Kalleshachar Neelima Nizampatnam department of Mathematics and Statistics College of ScienceImam Mohammad Ibn Saud Islamic University(IMSIU)Riyadh11564Saudi Arabia department of Mathematics College of ScienceKing Saud UniversityRiyadh11989Saudi Arabia department of Mathematics Amity School of Applied SciencesAmity University RajasthanJaipur302002RajasthanIndia department of Studies in Mathematics Davangere UniversityDavangere577002India department of Electronics and Communication Engineering Amrita School of EngineeringAmrita Vishwa VidyapeethamBengaluru560035India

Assessing the behaviour and concentration of waste pollutants deposited between two parallel plates is essential for effective environmental *** the effectiveness of treatment methods in reducing pollution scales is made easier by analysing waste discharge *** waste discharge concentration analysis is useful for assessing how effectively wastewater treatment techniques reduce pollution *** study aims to explore the Casson micropolar fluid flow through two parallel plates with the influence of pollutant concentration and thermophoretic particle *** explore the mass and heat transport features,thermophoretic particle deposition and thermal radiation are *** governing equations are transformed into ordinary differential equations with the help of suitable similarity *** Runge-Kutta-Fehlberg’s fourthfifth order technique and shooting procedure are used to solve the reduced set of equations and boundary *** integration of a neural network model based on the Levenberg-Marquardt algorithm serves to improve the accuracy of predictions and optimize the analysis of *** outcomes are displayed to analyze the characteristics of the relevant dimensionless parameters in the current *** reveal that concentration upsurges as the micropolar parameter *** concentration reduces with an upsurge in the thermophoretic *** upsurge in the external pollutant source variation and the local pollutant external source parameters enhances mass *** surface drag force declines for improved values of porosity and micropolar parameters.

关键词： Micropolar fluid thermal radiation porous medium thermophoretic particle deposition waste discharge concentration

Utilizing engineered extracellular vesicles as delivery vectors in the management of ischemic stroke:a special outlook on mitochondrial delivery

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Neural Regeneration Research 2025年第8期20卷 2181-2198页

作者： Jiali Chen Yiyang Li Xingping Quan Jinfen Chen Yan Han Li Yang Manfei Zhou Greta Seng Peng Mok Ruibing Wang Yonghua Zhao Institute of Chinese Medical sciences State Key Laboratory of Quality Research in Chinese MedicineUniversity of MacaoTaipaMacao Special Administrative RegionChina department of Pharmacy Hunan Provincial People’s Hospitalthe First Affiliated Hospital of Hunan Normal UniversityChangshaHunan ProvinceChina department of Electrical and Computer Engineering University of MacaoTaipaMacao Special Administrative RegionChina department of Pharmaceutical sciences Faculty of Health SciencesUniversity of MacaoTaipaMacao Special Administrative RegionChina

Ischemic stroke is a secondary cause of mortality worldwide,imposing considerable medical and economic burdens on *** vesicles,serving as natural nanocarriers for drug delivery,exhibit excellent biocompatibility in vivo and have significant advantages in the management of ischemic ***,the uncertain distribution and rapid clearance of extracellular vesicles impede their delivery *** utilizing membrane decoration or by encapsulating therapeutic cargo within extracellular vesicles,their delivery efficacy may be greatly ***,previous studies have indicated that microvesicles,a subset of large-sized extracellular vesicles,can transport mitochondria to neighboring cells,thereby aiding in the restoration of mitochondrial function post-ischemic *** extracellular vesicles have also demonstrated the capability to transfer mitochondrial components,such as proteins or deoxyribonucleic acid,or their sub-components,for extracellular vesicle-based ischemic stroke *** this review,we undertake a comparative analysis of the isolation techniques employed for extracellular vesicles and present an overview of the current dominant extracellular vesicle modification *** the complex facets of treating ischemic stroke,we also delineate various extracellular vesicle modification approaches which are suited to different facets of the treatment ***,given the burgeoning interest in mitochondrial delivery,we delved into the feasibility and existing research findings on the transportation of mitochondrial fractions or intact mitochondria through small extracellular vesicles and microvesicles to offer a fresh perspective on ischemic stroke therapy.

关键词： delivery engineering extracellular vesicles identification ischemic stroke isolation mitochondria targeting strategy therapeutic effects

Salicylic acid and heat acclimation pretreatment protects Laminaria japonica sporophyte(Phaeophyceae) from heat stress

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Chinese Journal of Oceanology and Limnology 2010年第4期28卷 924-932页

作者：周斌唐学玺王悠 Laboratory of Ecology College of Marine Life SciencesOcean University of China

Possible mediatory roles of heat acclimation and salicylic acid in protecting the sporophyte of marine macroalga Laminaria japonica(Phaeophyceae) from heat stress were *** stress resulted in oxidative injury in the kelp *** heat stress significant accumulation of hydrogen peroxide(H_2O_2) and malonaldehyde(MDA),a membrane lipid peroxidation product,and a drastic decrease in chlorophyll a content were *** of the enzymatic antioxidant system was drastically affected by heat *** activity of superoxide dismutase(SOD) was significantly increased while peroxidase(POD),catalase(CAT) and glutathione peroxidase(GPX) were greatly inhibited and,simultaneously,phenylalanine ammonia-lyase was activated while polyphenol oxidase(PPO) was *** heat acclimation pretreatment and exogenous application of salicylic acid alleviated oxidative damage in kelp *** receiving heat acclimation pretreatment and exogenous salicylic acid prior to heat stress exhibited a reduced increase in H_2O_2 and MDA content,and a lower reduction in chlorophyll a *** with heat acclimation and salicylic acid elevated activities of SOD,POD,CAT,GPX and *** these results collectively,we speculate that the inhibition of antioxidant enzymes is a possible cause of the heat-stress-induced oxidative stress in ***,and enhanced thermotolerance may be associated,at least in part,with the elevated activity of the enzymatic antioxidant system.

关键词： Laminaria japonica salicylic acid heat acclimation thermotolerance oxidative stress antioxidant system

Application of restriction site amplified polymorphism (RSAP) to genetic diversity in Saccharina japonica

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Chinese Journal of Oceanology and Limnology 2013年第4期31卷 830-834页

作者：赵翠刘翠李威池姗冯荣芳刘涛 Laboratory of Genetics and Breeding of marine Organism College of Marine Life SciencesOcean University of China

Restriction site amplified polymorphism (RSAP) was used, for the first time, to analyze the genetic structure and diversity of four, mainly cultivated, varieties of the brown alga, Saccharina japonica. Eighty-eight samples from varieties "Rongfu", "Fujian", "Ailunwan" and "Shengchanzhong" were used for the genetic analyses. One hundred and ninety-eight bands were obtained using eight combinations of primers. One hundred and ninety-one (96.46%) were polymorphic bands. Nei's genetic diversity was 0.360, and the coefficient of genetic differentiation was 0.357. No inbreeding-type recession was found in the four brown alga varieties and the results of the "Ailunwan" variety using samples from 2 years showed that the variety was becoming less diverse during the selection inherent in the breeding program. Genetic diversity and cluster analyses results were consistent with these genetic relationships. The results show the RSAP method is suitable for genetic analysis. Continuous inbreeding and selection could reduce the genetic diversity effectively; therefore periodical supervision is required.

关键词： Saccharinajaponica restriction site amplified polymorphism genetic diversity

Effects of heavy metals (Pb^(2+) and Cd^(2+)) on the ultrastructure, growth and pigment contents of the unicellular cyanobacterium Synechocystis sp. PCC 6803

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Chinese Journal of Oceanology and Limnology 2009年第2期27卷 383-388页

作者： K. K. I. U. Arunakumara 张学成 College of marine life sciences Ocean University of China department of Crop Science Faculty of AgricultureUniversity of Ruhuna

The unicellular cyanobacterium Synechocystis sp. PCC 6803, a model organism known for its unique combination of highly desirable molecular genetic, physiological and morphological characteristics, was employed in the present study. The species was cultured in BG11 liquid medium contained various initial concentrations of Pb^2＋ and Cd〉（0, 0.5, 1, 2, 4, 6 and 8 mg/L）. The experiment was conducted for six days and the metal induced alterations in the ultrastructure, growth and pigment contents were assessed. Alterations in the ultrastructure of the Synechocystis sp. PCC 6803 cells became evident with the increased （〉4 mg/L Pb^2＋） metal concentration. The photosynthetic apparatus （thylakoid membranes） were found to be the worst affected. Deteriorated or completely destroyed thylakoid membranes have made large empty spaces in the cell interior. In addition, at the highest concentration （8 mg/L pb^2＋）, the polyphosphate granules became more prominent both in size and number. Despite the initial slight stimulations （0.2, 3.8 and 6.5% respectively at 0.5, 1 and 2 mg/L pb^2＋）, both metals inhibited the growth in a dose-dependent manner as incubation progressed. Pigment contents （chlorophyll a, 13 carotene and phycocyanin） were also decreased with increasing metal concentration. Cells exposed to 6 mg/L Pb^2＋, resulted in 36.56, 37.39 and 29.34% reductions of chlorophyll a, 13 carotene and phycocyanin respectively over the control. Corresponding reductions for the same CdZ＋concentrations were 57.83, 48.94 and 56.90%. Lethal concentration （96 h LC50） values （3.47 mg/L Cd^2＋ and 12.11 mg/L Pb^2＋） indicated that Synechocystis sp. PCC 6803 is more vulnerable to Cd^2＋ than Pb^2＋.

关键词： growth pigment contents Synechocystis sp. PCC 6803 ultrastructure

The Appearance of Ulva laetevirens(Ulvophyceae, Chlorophyta) in the Northeast Coast of the United States of America

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Journal of Ocean University of China 2014年第5期13卷 865-870页

作者： MAO Yunxiang Jang Kyun Kim Roderick Wilson Charles Yarish department of Ecology and Evolutionary Biology University of Connecticut at Stamford Key Laboratory of marine Genetics and Breeding (MOE) College of Marine Life Sciences Ocean University of China department of marine sciences University of Connecticut at Stamford

Introduced species may outcompete or hybridize with native species, resulting in the loss of native biodiversity or even alteration of ecosystem processes. In this study, we reported an alien distromatic Ulva species, which was found in an embayment(Holly Pond) connected with Long Island Sound, USA. The morphological and anatomical observations in combination with molecular data were used for its identification to species. Anatomy of collected specimens showed that the cell shape in rhizoidal and basal regions was round and the marginal teeth along the basal and median region were not found. These characteristics were primarily identical to the diagnostic characteristics of Ulva laetevirens Areschoug(Chlorophyta). The plastid-encoding tufA and nucleus-encoding ITS1 were used for its molecular identification. Phylogenetic analysis for the tufA gene placed the specimens from Holly Pond in a well-supported clade along with published sequences of U. laetevirens identified early without any sequence divergence. In ITS tree, the sample also formed well-supported clades with the sequences of U. laetevirens with an estimated sequence divergence among the taxa in these clades as low as 1%. These findings confirmed the morpho-anatomical conclusion. Native to Australia, this species was reported in several countries along the Mediterranean coast after the late of 1990 s. This is the first time that U. laetevirens is found in the northeast coast of United States and the second record for Atlantic North America.

关键词： Ulva laetevirens Areschoug introduced seaweed Long Island Sound USA ITS tufA

Early development of Costaria costata(***) Saunders and cultivation trials

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Chinese Journal of Oceanology and Limnology 2010年第4期28卷 731-737页

作者：付刚刘吉东王高歌姚建亭王秀良段德麟 College of marine life sciences Ocean University of China Institute of Oceanology Chinese Academy of Sciences

Costaria costata(***) Saunders is one of common kelps distributed in many coastal areas worldwide;however,in China,no reports have been made on cultivation of the *** investigate potential cultivation of the species in the northern part of China,trials on isolation and preservation of the gametophytes were conducted using *** from Korea;growth and development of the gametophytes were *** showed that at 10±1°C,60 μmol m-2s-1 and 12:12 h(L:D),freshly released zoospores settled down within 1 hour,and then developed into the primary cell during the following 2 *** a vegetative growth phase lasting 6-8 days,female gametophytes became 3-4 times larger in diameter than that of the primary cell,but still remained at a unicellular stage,while male gametophytes divided into 4-10 cells with only a slight change in *** occurred within 10 days after the zoospores were released from the sporangia,and the apical and basal tissues of the juvenile sporophyte divided and differentiated into the blade and *** and irradiance influenced gametophytic vegetative growth and developmental ***,low irradiance(15 μmol m-2s-1 and 30 μmol m-2s-1) was unfavorable to the induction of fertility,but it enhanced female gametophyte *** optimal conditions for vegetative growth were 15°C and 30 μmol *** transplantation of the juvenile seedlings and after eight months cultivation,the harvested mature blade reached 194 cm in length and 32.7 cm in *** study proves that it is feasible to implement propagation and large scale cultivation of *** in northern China.

关键词： Costaria costata gametophyte growth development cultivation