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Establishment of a system for screening and identification of novel bactericide targets in the plant pathogenic bacterium Xanthomonas oryzae pv. oryzae using Tn-seq and SPR

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Journal of Integrative Agriculture 2024年第5期23卷 1580-1592页

作者： Chaoyue Pang Ling Jin Haoyu Zang Damalk Saint-Claire SKoklannou Jiazhi Sun Jiawei Yang Yongxing Wang Liang Xu Chunyan Gu Yang Sun Xing Chen Yu Chen School of plant protection Anhui Agricultural UniversityHefei 230036China Institute of plant protection and Agro-Products Safety Anhui Academy of Agricultural SciencesHefei 230031China Anhui Province Laboratory for Green Pesticide Development and Application School of Plant ProtectionAnhui Agricultural UniversityHefei230036China Hefei Research Center State Key Laboratory for Biology of Plant Diseases and Insect PestsHefei 230036China Key Laboratory of Integrated Crop Pest Management of Anhui Province School of Plant ProtectionAnhui Agricultural University Hefei230036China Shandong Vicome Greenland Chemical Co. Ltd.Jinan 250204China

Xanthomonas spp. cause severe bacterial diseases. However, effective strategies for prevention and management of these diseases are scarce. Thus, it is necessary to improve the efficiency of control of diseases caused by Xanthomonas. In this study, Xanthomonas oryzae pv. oryzae(Xoo), which causes rice bacterial leaf blight, has been studied as a representative. A transposon insertion library of Xoo, comprising approximately 200,000 individual insertion mutants, was generated. Transposon sequencing data indicated that the mariner C9 transposase mapped at 35.7–36.4% of all potential insertion sites, revealing 491 essential genes required for the growth of Xoo in rich media. The results show that, compared to the functions of essential genes of other bacteria, the functions of some essential genes of Xoo are unknown, 25 genes might be dangerous for the Xanthomonas group, and 3 are specific to Xanthomonas. High-priority candidates for developing broad-spectrum, Xanthomonas-specific, and environment-friendly bactericides were identified in this study. In addition, this study revealed the possible targets of dioctyldiethylenetriamine using surface plasmon resonance(SPR) in combination with high performance liquid chromatography–mass spectrometry(HPLC–MS). The study also provided references for the research of some certain bactericides with unknown anti-bacterial mode of action. In conclusion, this study urged a better understanding of Xanthomonas,provided meaningful data for the management of bacterial leaf blight, and disclosed selected targets of a novel bactericide.

关键词： rice bacterial leaf blight Xanthomonas essential genes Tn-seq dioctyldiethylenetriamine

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Juvenile hormone induces reproduction via miR-1175-3p in the red imported fire ant, Solenopsis invicta

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insect Science 2024年第2期31卷 371-386页

作者： Jie Chen Ziying Guan Yunjie Ma Qingxing Shi Ting Chen Muhammad Irfan WarisLihua Lyu Lihua Lyu Yongyue Lu Guojun Qi Institute of plant protection Guangdong Academy of Agricultural SciencesKey Laboratory of Green Prevention and Control on Fruits and Vegetables in South China Ministry of Agriculture and Rural AffairsGuangdong Provincial Key Laboratory of High Technology for Plant ProtectionGuangzhouChina department of Entomology College of Plant ProtectionSouth China Agricultural UniversityGuangzhouChina

Juvenile hormone(JH)acts in the regulation of caste differentiation between queens and workers(i.e.,with or without reproductive capacity)during vitellin synthesis and oogenesis in social ***,the regulatory mechanisms have not yet been ***,we identified a highly expressed microRNA(miRNA),miR-1175-3p,in the red imported fire ant,Solenopsis *** found that miR-1175-3p is prominently present in the fat bodies and ovaries of ***,miR-1175-3p interacts with its target gene,broad-complex core(Br-C),in the fat *** utilizing miR-1175-3p agomir,we successfully suppressed the expression of the Br-C protein in queens,resulting in reduced vitellogenin expression,fewer eggs,and poorly developed ***,decreasing miR-1175-3p levels led to the increased expression of Br-C and vitellogenin in workers,triggering the“re-development”of the ***,when queens were fed with JH,the expression of miR-1175-3p decreased,whereas the expression of vitellogenin-2 and vitellogenin-3 ***,the suppression of fertility in queens caused by treatment with agomir miR-1175-3p was completely rescued by the increased vitellogenin expression induced by being fed with *** results suggest the critical role of miR-1175-3p in JH-regulated reproduction,shedding light on the molecular mechanism underlying miRNA-mediated fecundity in social insects and providing a novel strategy for managing ***.

关键词： juvenile hormone miR-1175-3p red imported fire ant reproduction social insects vitellogenin

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Odorant receptor orthologues from moths display conserved responses to cis-jasmone

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insect Science 2024年第4期31卷 1107-1120页

作者： Xiao-Qing Hou Zhongqiang Jia Dan-Dan Zhang Guirong Wang Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureSynthetic Biology Laboratory of the Ministry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhenGuangdong ProvinceChina State Key Laboratory for Biology of plant Diseases and insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijingChina department of Biology Lund UniversityLundSweden

In insects,the odorant receptor(OR)multigene family evolves by the birth-and-death evolutionary model,according to which the OR repertoire of each species has undergone specific gene gains and losses depending on their chemical environment,resulting in taxon-specific OR lineage radiations with different sizes in the phylogenetic *** the general divergence in the gene family across different insect orders,the ORs in moths seem to be genetically conserved across species,clustered into 23 major clades containing multiple orthologous groups with single-copy gene from each *** hypothesized that ORs in these orthologous groups are tuned to ecologically important compounds and functionally ***-Jasmone is one of the compounds that not only primes the plant defense of neighboring receiver plants,but also functions as a behavior regulator to various *** test our hypothesis,using Xenopus oocyte recordings,we functionally assayed the orthologues of BmorOR56,which has been characterized as a specific receptor for *** results showed highly conserved response specificity of the BmorOR56 orthologues,with all receptors within this group exclusively responding to *** is supported by the dN/dS analysis,showing that strong purifying selection is acting on this ***,molecular docking showed that the ligand binding pockets of BmorOR56 orthologues to cis-jasmone are *** together,our results suggest the high conservation of OR for ecologically important compounds across Heterocera.

关键词： cis-jasmone evolutionary conservation functional characterization molec-ular docking moths purifying selection

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Gene cloning,protein expression,and enzymatic characterization of a double-stranded RNA degrading enzyme in Apolygus lucorum

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insect Science 2024年第1期31卷 119-133页

作者： Jie-Yu Zhang Jing Zhao Keyan Zhu-Salzman Qin-Qin Ji Yi-Ping Jiang Liu-Bin Xiao De-Jin Xu Guang-Chun Xu Lin-Quan Ge Yong-An Tan College of plant protection Yangzhou UniversityYangzhouJiangsu ProvinceChina Institute of plant protection Jiangsu Academy of Agricultural SciencesNanjingJiangsu ProvinceChina department of Entomology Texas A&M UniversityCollege StationTXUSA Taizhou Customs of the People's Republic of China TaizhouJiangsu ProvinceChina

RNA interference(RNAi)is a powerful tool that post-transcriptionally silences target genes in eukaryotic ***,silencing efficacy varies greatly among different insect ***,we met with little success when attempting to knock down genes in the mirid bug Apolygus lucorum via dsRNA *** disappearance of double-stranded RNA(dsRNA)could be a potential factor that restricts RNAi ***,we found that dsRNA can be degraded in midgut fluids,and a dsRNase of ***(AldsRNase)was identified and *** alignment indicated that its 6 key amino acid residues and the Mg2+-binding site were similar to those of other insects’*** signal peptide and endonuclease non-specific domain shared high sequence identity with the brown-winged green stinkbug Plautia stali *** showed high salivary gland and midgut expression and was continuously expressed through the whole life cycle,with peaks at the 4th instar ecdysis in the whole *** purified AldsRNase protein obtained by heterologously expressed can rapidly degrade *** comparing the substrate specificity of AldsRNase,3 specific substrates(dsRNA,small interfering RNA,and dsDNA)were all degraded,and the most efficient degradation is ***,immunofluorescence revealed that AldsRNase was expressed in the cytoplasm of midgut *** cloning and functional study of AldsRNase,the enzyme activity and substrate specificity of the recombinant protein,as well as the subcellular localization of nuclease,the reason for the disappearance of dsRNA was explained,which was useful in improving RNAi efficiency in *** and related species.

关键词： Apolygus lucorum cloning dsRNase enzymatic activity protein expression

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Classical taxonomy,molecular phylogeny and genetic analysis of the genus Exitianus Ball,1929(Hemiptera:Cicadellidae:Deltocephalinae)from Egypt

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Zoological Systematics 2024年第2期49卷 143-157页

作者： Rabab M.El-Hady Ahmed F.El-Sayed Survey&Taxonomy department Plant Protection Research InstituteAgriculture Research CentreGizaEgypt Microbial Genetics department Biotechnology Research InstituteNational Research CentreGizaEgypt Egypt Center for Research and Regenerative Medicine(ECRRM) CairoEgypt

Egyptian species of the leafhopper genus Exitianus Ball,1929,***(St?l,1855),***(Distant,1908),and *** Ross,1968 are ***,morphological descriptions,and a key for their identification are *** this study,we used molecular techniques to confirm morphological identification and detect phylogeny among the three Exitianus *** partial nucleotide sequences of the amplified products obtained were determined by Macrogen *** nucleotide sequences of 28S rDNA and COX genes of the EGY-ARC-9,EGY-ARC-4,and EGY-ARC-5 were determined by Macrogen Korea,blasted into BLAST at the National Center for biotechnology Information website(NCBI)and compared with those deposited in the GenBank DNA *** results represented the homology percentage between the partial sequences of the 28S rRNA and COX genes from each species and related species obtained from GenBank DNA *** morphological identifications of the three Exitianus species were confirmed by molecular characterization and sequencing of 28S rDNA and COX genes and identified as ***,***,and ***,their sequences of 28srDNA and COX genes were deposited in GenBank with an accession number(LC670610,LC670607,and OQ196105)for 28srDNA gene and(LC775357,LC775358,and LC775359)for the COX gene.

关键词： Auchenorrhyncha Chaismini taxonomic 28S rDNA phylogenetic

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A dicistrovirus increases pupal mortality in Spodoptera frugiperda by suppressing protease activity and inhibiting larval diet consumption

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Journal of Integrative Agriculture 2024年第8期23卷 2723-2734页

作者： Meixue Sun Tong Li Yingjie Liu Kenneth Wilson Xingyu Chen Robert I.Graham Xianming Yang Guangwei Ren Pengjun Xu Key Laboratory of Tobacco Pest Monitoring&Integrated Management Tobacco Research InstituteChinese Academy of Agricultural SciencesQingdao 266101China Institute of plant protection Henan Academy of Agricultural SciencesZhengzhou 450000China Staff Development Institute of China National Tobacco Corporation Zhengzhou 450000China Lancaster Environment Centre Lancaster UniversityLancaster LA14YWUK department of Rural Land Use Scotland’s Rural CollegeCraibstone CampusAberdeen AB01ABUK State Key Laboratory for Biology of plant Diseases and insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China

Understanding interactions between viruses and their hosts is conducive to enabling better application of viruses as biocontrol *** viruses carried by parasitic wasps enhance the parasitic efficiency of wasp-larvae by protecting them against the immune system of their Lepidopteran ***,the relationship between prey pests and viruses found in predatory natural enemies remains ***,we report the interaction between Arma chinensis virus-1(AcV-1),originally isolated from a predatory natural enemy,Arma chinensis(Hemiptera:Pentatomidae),and one of its prey species,Spodoptera frugiperda(Lepidoptera:Noctuidae).The results showed that the AcV-1 virus appeared harmful to the novel host *** by inhibiting larval diet consumption and increasing pupal ***,sequencing data indicated that the virus altered the gene expression profiles of *** analysis showed that the proteasome and phagosome pathways related to protein degradation and immune response were significantly *** the expression levels of digestive enzyme genes did not change significantly,the total protease activity of AcV-1 virus-positive individuals was significantly decreased,suggesting that the virus inhibited diet consumption of *** via the down-regulation of digestive enzyme *** results indicate that a virus initially isolated in a predatory natural enemy can decrease the fitness of its prey *** virus was found to impact the host proteasome and phagosome pathways related to protein degradation and immunity,providing a potential mechanism to enhance controlling efficiency.

关键词： Arma chinensis virus-1 diet consumption fitness transcriptome protease activity

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A glycine-rich nuclear effector VdCE51 of Verticillium dahliae suppresses plant immune responses by inhibiting the accumulation of GhTRXH2

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The Crop Journal 2024年第4期12卷 1137-1149页

作者： Chi Li Yingqi Huang Wenjing Shang Jieyin Chen Steven J.Klosterman Krishna V.Subbarao Jun Qin Xiaoping Hu State Key Laboratory of Crop Stress Resistance and High-Efficiency Production Key Laboratory of Plant Protection Resources and Pest Management of Ministry of EducationKey Laboratory of Integrated Pest Management on the Loess Plateau of Ministry of Agriculture and Rural AffairsCollege of Plant ProtectionNorthwest A&F UniversityYangling 712100ShaanxiChina State Key Laboratory for Biology of plant Diseases and insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China Western Agricultural Research Center Chinese Academy of Agricultural SciencesChangji 831100XinjiangChina Agricultural Research Service United States Department of AgricultureSalinasCA 93905USA department of plant Pathology University of CaliforniaDavisc/o United States Agricultural Research StationSalinasCA 93905USA

Verticillium dahliae is an important soil-borne fungal pathogen that causes great yield losses in many cash *** of this fungus are known to regulate plant immunity but the mechanism much remains unclear.A glycine-rich nuclear effector,VdCE51,was able to suppress immune responses in tobacco against Botrytis cinerea and Sclerotinia *** effector was a required factor for full virulence of ***,and its nuclear localization was a requisite for suppressing plant *** thioredoxin GhTRXH2,identified as a positive regulator of plant immunity,was a host target of *** findings show a virulence regulating mechanism whereby the secreted nuclear effector VdCE51 interferes with the transcription of PR genes,and the SA signaling pathway by inhibiting the accumulation of GhTRXH2,thus suppressing plant immunity.

关键词： Verticillium dahliae plant immunity Effector Thioredoxin

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Crop antiviral defense: Past and future perspective

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Science China Life Sciences 2024年

作者： Zhirui Yang Guangyao Li Yongliang Zhang Fangfang Li Tao Zhou Jian Ye Xianbing Wang Xiaoming Zhang Zongtao Sun Xiaorong Tao Ming Wu Jianguo Wu Yi Li The State Key Laboratory of Protein and plant Gene Research School of Life Sciences Peking University State Key Laboratory of plant Environmental Resilience Frontiers Science Center for Molecular Design Breeding College of Biological Sciences China Agricultural University State Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops Vector-borne Virus Research Center College of Plant Protection Fujian Agriculture and Forestry University State Key Laboratory for Biology of plant Diseases and insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences State Key Laboratory for Agro-biotechnology and department of plant Pathology China Agricultural University State Key Laboratory of plant Genomics Institute of Microbiology Chinese Academy of Sciences State Key Laboratory of Integrated Management of Pest insects and Rodents Institute of Zoology Chinese Academy of SciencesCAS Center for Excellence in Biotic Interactions University of Chinese Academy of Sciences State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province Institute of Plant Virology Ningbo University department of plant Pathology The Key Laboratory of Plant Immunity Nanjing Agricultural University

Viral pathogens not only threaten the health and life of humans and animals but also cause enormous crop yield losses and contribute to global food insecurity. To defend against viral pathogens, plants have evolved an intricate immune system to perceive and cope with such attacks. Although most of the fundamental studies were carried out in model plants, more recent research in crops has provided new insights into the antiviral strategies employed by crop plants. We summarize recent advances in understanding the biological roles of cellular receptors, RNA silencing, RNA decay, hormone signaling, autophagy, and ubiquitination in manipulating crop host-mediated antiviral responses. The potential functions of circular RNAs, the rhizosphere microbiome, and the foliar microbiome of crops in plant-virus interactions will be fascinating research directions in the future. These findings will be beneficial for the development of modern crop improvement strategies.

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plant virology in the 21st century in China:Recent advances and future directions

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Journal of Integrative plant Biology 2024年第3期66卷 579-622页

作者： Jianguo Wu Yongliang Zhang Fangfang Li Xiaoming Zhang Jian Ye Taiyun Wei Zhenghe Li Xiaorong Tao Feng Cui Xianbing Wang Lili Zhang Fei Yan Shifang Li Yule Liu Dawei Li Xueping Zhou Yi Li State Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops Vector-borne Virus Research CenterCollege of Plant ProtectionFujian Agriculture and Forestry UniversityFuzhou 350002China State Key Laboratory of plant Environmental Resilience and Ministry of Agriculture Key Laboratory of Soil Microbiology College of Biological SciencesChina Agricultural UniversityBeijing 100193China State Key Laboratory for Biology of plant Diseases and insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China State Key Laboratory of Integrated Management of Pest insects and Rodents Institute of ZoologyChinese Academy of SciencesBeijing 100101China CAS Center for Excellence in Biotic Interactions University of Chinese Academy of SciencesBeijing 100049China State Key Laboratory of plant Genomics Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China State Key Laboratory of Rice Biology Institute of BiotechnologyZhejiang UniversityHangzhou 310058China department of plant Pathology The Key Laboratory of Plant ImmunityNanjing Agricultural UniversityNanjing 210095China State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Institute of Plant VirologyNingbo UniversityNingbo 315211China MOE Key Laboratory of Bioinformatics Center for Plant BiologySchool of Life SciencesTsinghua UniversityBeijing 100084China State Key Laboratory of Protein and plant Gene Research School of Life SciencesPeking UniversityBeijing 100871China

plant viruses are a group of intracellular pathogens that persistently threaten global food *** advances in plant virology have been achieved by Chinese scientists over the last 20 years,including basic research and technologies for preventing and controlling plant viral ***,we review these milestones and advances,including the identification of new crop-infecting viruses,dissection of pathogenic mechanisms of multiple viruses,examination of multilayered interactions among viruses,their host plants,and virus-transmitting arthropod vectors,and in-depth interrogation of plantencoded resistance and susceptibility ***,various plant virus-based vectors have also been successfully developed for gene function studies and target gene expression in *** also recommend future plant virology studies in China.

关键词： China emerging plant viruses plant virology plant resistance/susceptibility to viral infection plant-virus transmission vector interactions plant virus-based vectors viral pathogenesis

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The cluster digging behavior of larvae confers trophic benefits to fitness in insects

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insect Science 2024年第3期31卷 870-884页

作者： Yujie Wu Qiang Wang Weikang Yang Sheng Zhang Chuan-Xi Mao Nana He Shaojie Zhou Chuanming Zhou Wei Liu School of plant protection Anhui Agricultural UniversityAnhui Province Key Laboratory of Crop Integrated Pest ManagementAnhui Province Engineering Laboratory for Green Pesticide Development and ApplicationHefeiChina College of plant protection Nanjing Agricultural UniversityNanjingChina School of Teacher Education Nanjng Xiaozhuang UniversityNanjingChina State Key Laboratory of Biocatalysis and Enzyme Engineering Hubei Province Key Laboratory of Biotechnology of Chinese Traditional MedicineNational&Local Joint Engineering Research Center of High-throughput Drug Screening TechnologySchool of Life ScienceHubei UniversityWuhanChina

Collective behaviors efficiently impart benefits to a diversity of species ranging from bacteria to *** larvae tend to cluster and form coordinated digging groups under crowded conditions,yet understanding the rules governing this behavior is in its *** primarily took advantage of the Drosophila model to investigate cooperative foraging ***,we report that Drosophila-related species and the black soldier fly have evolved a conserved strategy of cluster digging in food ***,we investigated relative factors,including larval stage,population density,and food stiffness and quality,that affect the cluster digging ***,oxygen supply through the posterior breathing spiracles is necessary for the organization of digging *** importantly,we theoretically devise a mathematical model to accurately calculate how the cluster digging behavior expands food resources by diving depth,cross-section area,and food *** found that cluster digging behavior approximately increases 2.2 fold depth,1.7-fold cross-section area,and 1.9 fold volume than control groups,*** of food sources significantly facilitates survival,larval development,and reproductive success of Drosophila challenged with competition for limited food resources,thereby conferring trophic benefits to fitness in ***,our findings highlight that the cluster digging behavior is a pivotal behavior for their adaptation to food scarcity,advancing a better understanding of how this cooperative behavior confers fitness benefits in the animal kingdom.

关键词： cluster digging determinant development fecundity larvae mathematical model

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