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Autochthonous halotolerant plant growth-promoting rhizobacteria promote bacoside A yield of Bacopa monnieri(L.)Nash and phytoextraction of salt-affected soil

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Pedosphere 2020年第5期30卷 671-683页

作者： Umesh PANKAJ Durgesh Narain SINGH Pooja MISHRA Pooja GAUR C.S.Vivek BABU Karuna SHANKER Rajesh Kumar VERMA department of Soil Science CSIR-Central Institute of Medicinal and Aromatic PlantsLucknow 226015(India) Division of Biotechnology CSIR-Central Institute of Medicinal and Aromatic PlantsLucknow 226015(India) Division of Microbial Technology CSIR-Central Institute o f Medicinal and Aromatic PlantsLucknow 226015(India) Analytical Chemistry department CSIR-Central Institute o f Medicinal and Aromatic PlantsLucknow 226015(India) Research Centre CSIR-Central Institute of Medicinal and Aromatic PlantsBangalore 560065(India)

Phytoremediation is a promising approach for reclamation of salt-affected *** is the most commonly used process,which exploits plants to absorb,immobilize,and accumulate salt in their *** this study,halotolerant plant growth-promoting rhizobacteria(PGPR)were isolated from the rhizosphere of wild grasses growing naturally in salt-affected areas of Lucknow,Uttar Pradesh(India)and were tested for their efficacies of salt-tolerance and plant growth-promoting(PGP)*** on 16S rRNA sequences,the most efficient halotolerant isolates possessing PGP traits were identified as Pseudomonas plecoglossicida(KM 233646),Acinetobacter calcoaceticus(KM 233647),Bacillus flexus(KM 233648),and Bacillus safensis(KM 233652).Application of these isolates as bio-inoculants significantly(Pplants,the soil physico-chemical properties of bio-inoculant-treated plants were *** shoot and root biomass(fresh and dry weights),soil enzymes,and soil nutrient parameters showed significant positive correlations with the shoot Na+:K+***,the halotolerant PGPR screened in this study could be useful for the reclamation of saline soils concomitant with improved plant growth and bacoside A yield.

关键词： bio-inoculant phytoremediation plant productivity soil physico-chemical property soil salinity

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Heteromeric and Homomeric Geranyl Diphosphate Synthases from Catharanthus roseus and Their Role in Monoterpene Indole Alkaloid Biosynthesis

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Molecular Plant 2013年第5期6卷 1531-1549页

作者： Avanish Rai Shachi S. Smita Anup Kumar Singh Karuna Shanker Dinesh A. Nagegowda CSIR-Central Institute of Medicinal and aromatic plants Research Centre Bangalore 560065 India CSIR-Central Institute of Medicinal and aromatic plants Lucknow 226015 India

Catharanthus roseus is the sole source of two most important monoterpene indole alkaloid （MIA） anti- cancer agents： vinblastine and vincristine. MIAs possess a terpene and an indole moiety derived from terpenoid and shikimate pathways, respectively. Geranyl diphosphate （GPP）, the entry point to the formation of terpene moiety, is a product of the condensation of isopentenyl diphosphate （IPP） and dimethylallyl diphosphate （DMAPP） by GPP synthase （GPPS）. Here, we report three genes encoding proteins with sequence similarity to large subunit （***） and small subunit （***） of heteromeric GPPSs, and a homomeric GPPSs. *** is a bifunctional enzyme producing both GPP and geranyl geranyl diphosphate （GGPP）, *** is inactive, whereas CrGPPS is a homomeric enzyme forming GPP. Co-expression of both subunits in Escherichia coil resulted in heteromeric enzyme with enhanced activity producing only GPR While *** and CrGPPS showed higher expression in older and younger leaves, respectively, *** showed an increasing trend and decreased gradually. Methyl jasmonate （MelA） treatment of leaves sig- nificantly induced the expression of only ***. GFP localization indicated that *** is plastidial whereas CrGPPS is mitochondrial. Transient overexpression of *** in C. roseus leaves resulted in increased vindoline, immediate monomeric precursor of vinblastine and vincristine. Although C. roseus has both heteromeric and homomeric GPPS enzymes, our results implicate the involvement of only heteromeric GPPS with *** regulating the GPP flux for MIA biosynthesis.

关键词： C. roseus monoterpene indole alkaloids geranyl diphosphate synthase heteromer homomer large subunit small subunit vindoline.

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Antimycobacterial natural products from Moroccan medicinal plants: Chemical composition, bacteriostatic and bactericidal profile of Thymus satureioides and Mentha pulegium essential oils

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Asian Pacific Journal of Tropical Biomedicine 2016年第10期6卷 836-840页

作者： Marwa Chraibi Abdellah Farah Sara Lebrazi Oumaima El Amine Mohammed Iraqui Houssaini Kawtar Fikri-Benbrahim Laboratory of Microbial Biotechnology Faculty of Science and Technology Sass Sidi Mohamed Ben Abdellah University Laboratory of aromatic plants Medicinal and Natural Substances National Institute of Medicinal and Aromatic PlantsPhytochemistry Department

Objective: To evaluate the susceptibility of Mycobacterium aurum and Mycobacterium smegmatis in vitro to the essential oils obtained from two medicinal plants: Thymus satureioides(T. satureioides) and Mentha pulegium(M. pulegium), and to study their chemical ***: The aerial parts of T. satureioides and M. pulegium(leaves and stems) were hydro-distillated using a Clevenger-type apparatus and essential oils were analyzed and identified by gas chromatography-mass spectrometry. Antimycobacterial screening of essential oils was performed on the basis of the inhibition zone diameter by disc diffusion method against two mycobacterial strains whereas the minimal inhibitory concentration and minimal bactericidal concentration were determined by using the micro-dilution ***: Chemical analysis of their aerial part's essential oils gave as major compounds,borneol(34.26%), carvacrol(31.21%) and thymol(3.71%) for T. satureioides and R(+)-pulegone(75.48%), carvone(6.66%) and dihydrocarvone(4.64%) for M. *** their antimycobacterial effect evaluation, using the micro-dilution method,indicated that minimal inhibitory concentration values of T. satureioides essential oil ranged from 0.062% to 0.015%(v/v) and from 0.125% to 0.031%(v/v) for M. pulegium respectively against Mycobacterium aurum and Mycobacterium ***: It is clearly evident from the results obtained that the Moroccan medicinal plants have great potential to be used as anti-tuberculosis agents. These findings may help scientists to undertake several research projects to discover useful natural product as new anti-tuberculosis drug.

关键词： Thymus satureioides Mentha pulegium Essential oil Chemical composition Antimycobacterial activity

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Rapid metabolic fingerprinting with the aid of chemometric models to identify authenticity of natural medicines: Turmeric, Ocimum, and Withania somnifera study

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Journal of Pharmaceutical Analysis 2023年第9期13卷 1041-1057页

作者： Samreen Khan Abhishek Kumar Rai Anjali Singh Saudan Singh Basant Kumar Dubey Raj Kishori Lal Arvind Singh Negi Nicholas Birse Prabodh Kumar Trivedi Christopher T.Elliott Ratnasekhar Ch Metabolomics Lab Council of Scientific and Industrial Research(CSIR)-Central Institute of Medicinal and Aromatic Plants(CIMAP)Lucknow226015India department of Crop Production&Protection Council of Scientific and Industrial Research(CSIR)-Central Institute of Medicinal and Aromatic Plants(CIMAP)Lucknow226015India Academy of Council of Scientific and Industrial Research(ACSIR) Ghaziabad201002India department of Biotechnology Council of Scientific and Industrial Research(CSIR)-Central Institute of Medicinal and Aromatic Plants(CIMAP)Lucknow226015India Genetics and Plant Breeding department Council of Scientific and Industrial Research(CSIR)-Central Institute of Medicinal and Aromatic Plants(CIMAP)Lucknow226015India department of Phytochemistry Council of Scientific and Industrial Research(CSIR)-Central Institute of Medicinal and Aromatic Plants(CIMAP)Lucknow226015India School of Biological Sciences Queen's UniversityBelfastBT95DLUK

Herbal medicines are popular natural medicines that have been used for *** use of alternative medicines continues to expand rapidly across the *** World Health Organization suggests that quality assessment of natural medicines is essential for any therapeutic or health care applications,as their therapeutic potential varies between different geographic origins,plant species,and *** of herbal medicines based on a limited number of secondary metabolites is not an ideal *** quality should be considered based on a complete metabolic profile,as their pharmacological activity is not due to a few specific secondary metabolites but rather a larger group of bioactive compounds.A holistic and integrative approach using rapid and nondestructive analytical strategies for the screening of herbal medicines is required for robust *** this study,a rapid and effective quality assessment system for geographical traceability,species,and variety-specific authenticity of the widely used natural medicines turmeric,Ocimum,and Withania somnifera was investigated using Fourier transform near-infrared(FT-NIR)spectroscopy-based metabolic *** different geographical origins of turmeric,five different Ocimum species,and three different varieties of roots and leaves of Withania somnifera were studied with the aid of machine learning *** good discrimination(R^(2)>0.98,Q^(2)>0.97,and accuracy=1.0)with sensitivity and specificity of 100%was achieved using this metabolic fingerprinting *** study demonstrated that FT-NIR-based rapid metabolic fingerprinting can be used as a robust analytical method to authenticate several important medicinal herbs.

关键词： Rapid metabolic fingerprinting Natural medicines FT-NIR Chemometric models

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Integrated Analysis of the Trees and Associated UnderCanopy Species in a Subalpine Forest of Western Himalaya,Uttarakhand, India

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Journal of Mountain Science 2015年第1期12卷 154-165页

作者： Vinod K.BISHT Chandra P.KUNIYAL Bhagwati P.NAUTIYAL Pratti Prasad Herbal Research and Development Institute department of Horticulture Aromatic and Medicinal Plants School of Earth Sciences and Natural Resources Management Mizoram University High Altitude plants Physiology Research Centre HNB Garhwal (Central) University

Subalpine forests are known as outstanding habitats due to co-existence of both temperate and alpine vegetation and are classic example of ecotonal zones. Limited but diverse physiognomy of trees inhabiting in subalpine forest results in variability within under-canopy habitat conditions. Studies were undertaken to assess population status, habitat preference and interferences to the trees and associated under-canopy herbs in a subalpine forest of western Himalaya. A total of lo woody and 23 under-canopy herbs were recorded in the selected subalpine forest area. At each stand, the number of tree species and under-canopy herbs ranged from 2 to 4 and 8 to lo respectively. Abies spectabilis, Acer caesium, Quercus floribunda, Q. semecarpifolia and Rhododendron arboreum were key tree species in this area. The density of main woody species was 280 to 119o individuals ha-1 at different stands. Herbaceous plants with rosette and clump growth habits were observed to have higher values for total basal cover and importance value index. Presence of some under- canopy herbs like; Dactylorhiza hatagirea, Malaxis muscifera, Picrorhiza kurrooa, Polygonatum cirrhifolium habitats also and Skimmia laureola showed that they are in the habitat specific specific. However, the presence of Frageria nubicola and Viola sp. was common in the selected stands. Felling of trees for timber, construction of temporary huts, fuel wood and lopping for fodder were main interferences for trees. On the other hand, trampling driven damage due to grazing, habitats degradation and overexploitation were observed key threats for under-canopy herbs. Integrated analysis including population studies, habitats preference and interferences to the trees and under-canopy herbs in this sensitive and important ecosystem will be useful for determining the conservation plans and ecosystem management.

关键词： Conservation Habitat preferences Interferences Management Population analysis

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Production,characterization and biological activities of acidic exopolysaccharide from marine Bacillus amyloliquefaciens 3MS 2017

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Asian Pacific Journal of Tropical Medicine 2017年第7期10卷 715-725页

作者： Samah A.El-Newary Abeer Y.Ibrahim Mohsen S.Asker Manal G.Mahmoud Mohamed E.El Awady department of Medicinal and aromatic plants Research National Research CentreDokkiCairoEgypt Microbial Biotechnology department National Research CentreDokkiCairoEgypt

Objective: To evaluate in-vitro antioxidant, anti-inflammatory and antitumor abilities against human breast adenocarcinoma(MCF7) and human prostate cancer(PC3) as well as the suppressor effect of bacterial exopolysaccharide(BAEPS) on Ehrlich ascites carcinoma(EAC). Methods: In-vitro antioxidants characters of BAEPS were determined using various methods, while anti-inflammatory activity was estimated against cyclooxygenase(COX-1 and COX-2). In-vitro study, anticancer against MCF7 and PC3 were assessed by the mitochondrial dependent reduction of yellow MTT. In in-vivo study against EAC progression, mice were inoculated with EAC cells and then were orally administered BAEPS at 200 mg/kg after 24 h(equals to 0.10 of determined LD50)/10 d. Results: BAEPS was acidic exopolysaccharide contained uronic acid(12.3%) and sulfate(22.8%) with constitution of glucose, galactose and glucuronic acid in a molar ratio1.6: 1.0: 0.9, respectively, with a molecular mass of 3.76伊104 g/mol. BAEPS appeared potent antioxidant characters as free radical scavenging, oxygen reactive species scavenging and metal chelation, while its reducing power was low. BAEPS showed selective anti-inflammatory activity against COX-2 than COX-1, COX-2 selective. BAEPS exhibited potent and selective effect to breast cell cancer MCF7, the death percentage was 65.20% with IC_(50)=70 μg/m L and IC_(90)=127.40 μg/m L. BAEPS decreased counted viable EAC cells and induced non-viable cells. BAEPS improved all assessed hematological parameters. These improvements were reflected in the increasing median survival time and significant increment(P<0.05) in life span. Conclusions: BAEPS has anti-tumor activity with a good margin of safety. The anti-tumor activity of BAEPS may be due to its content from sulfated groups and uronic acids and they have antioxidant and anti-inflammatory properties.

关键词： Antioxidant Anti-inflammatory Anti-tumor Ehrlich ascites carcinoma Exopolysaccharide Bacillus amyloliquefaciens

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Identification, occurrence, and validation of DRE and ABRE Cis-regulatory motifs in the promoter regions of genes of Arabidopsis thaliana

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Journal of Integrative Plant Biology 2014年第4期56卷 388-399页

作者： Sonal Mishra Aparna Shukla Swati Upadhyay Sanchita Pooja Sharma Seema Singh Ujjal J.Phukan Abha Meena Feroz Khan Vineeta Tripathi Rakesh Kumar Shukla Ashok Shrama Biotechnology Division CSIR-Central Institute of Medicinal and Aromatic Plants A Laboratory Under Council of Scientific and Industrial Research Metabolic and Structural Biology Division CSIR-Central Institute of Medicinal and Aromatic Plants A Laboratory Under Council of Scientific and Industrial Research Botany department CSIR-Central Drug Research Institute (CSIR-CDRI)

plants posses a complex co-regulatory network which helps them to elicit a response under diverse adverse conditions. We used an in silico approach to identify the genes with both DRE and ABRE motifs in their promoter regions in Arabidopsis thaliana. Our results showed that Arabidopsis contains a set of 2,052 genes with ABRE and DRE motifs in their promoter regions. Approximately 72~o or more of the total predicted 2,052 genes had a gap distance of less than 40o bp between DRE and ABRE motifs. For positional orientation of the DRE and ABRE motifs, we found that the DR form （one in direct and the other one in reverse orientation） was more prevalent than other forms. These predicted 2,o52 genes include 155 transcription factors. Using microarray data from The Arabidopsis Information Resource （TAIR） database, we present 44 transcription factors out of 155 which are upregulated by more than twofold in response to osmotic stress and ABA treatment. Fifty-one transcripts from the one predicted above were validated using semiquantitative expression analysis to support the microarray data in TAIR. Taken together, we report a set of genes containing both DRE and ABRE motifs in their promoter regions in A. thaliana, which can be useful to understand the role of ABA under osmotic stress condition.

关键词： Abscisic acid abscisic acid-responsive elements dehydration-responsive element osmotic stress response

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Comparative assessment for removal of anionic dye from water by different waste-derived biochar vis a vis reusability of generated sludge

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Biochar 2022年第1期4卷 986-1002页

作者： Mayank Singh MohdAhsan Versha Pandey Anupama Singh Disha Mishra Neerja Tiwari Pooja Singh Tanmoy Karak Puja Khare Crop Production and Protection DivisionCSIR-Central Institute of Medicinal and Aromatic PlantsLucknow 226015India Phytochemistry Division CSIR-Central Institute of Medicinal and Aromatic PlantsLucknow 226015India Upper Assam Advisory Centre Tea Research AssociationDikomDibrugarh 786101AssamIndia Academy of Scientific and Innovative Research(AcSIR) GaziabadIndia Biotechnology Division Central Institute of Medicinal and Aromatic PlantsLucknow 226015India

In this study,four biochars prepared from different crop residue waste *** bagasse(SBB),coconut shell(CNB),paddy straw(PDB),and distilled waste of lemongrass(LGB)were evaluated for removal of Remazol Brilliant Blue R from the aqueous *** RBBR adsorption capacities of biochar were 97-79%for SBB,99.9-99.47%for CNB,66.1-48%for PDB,and 78-68%for LGB,dominantly controlled by their aromaticity and mineral *** Langmuir and Freundlich isotherms and pseudo-second-order kinetic models have described the chemisorption of RBBR on biochar *** thermodynamic data suggested that adsorption was spontaneous and *** biochars demonstrated excellent reusability(till four cycles with 50-61%regeneration).The purified water and biochar dye sludge demonstrated no *** findings obtained in this study may provide supports for the potential of biochars for anionic dye removal from water and utilization of generated sludge for zero waste-producing technologies in the future.

关键词： Biochar Remazol Brilliant Blue R Dye Adsorption Biochar dye sludge

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Polyphenolic content and antioxidant activity of some wild Saudi Arabian asteraceae plants

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Asian Pacific Journal of Tropical Medicine 2014年第7期7卷 545-551页

作者： Abdelaaty A.Shahat Abeer Y.Ibrahim Mansour S.Elsaid Pharmacognosy department College of PharmacyKing Saud University Phytochemistry department National Research Centre Medicinal and aromatic plants Research Dept. National Research Centre

Objective:To study the antioxidant properties of crude extract of different Asteraceae ***:The antioxidant properties of six extracts were evaluated using different antioxidant tests,including free radical scavenging,reducing power,metal chelation,superoxide anion radical scavenging,total antioxidant capacity and inhibition of lipid peroxidation ***:Picris cyanocarpa(***)and Anthemis deserti(***)had powerful antioxidant properties as radical scavenger,reducing agent and superoxide anion radical scavenger while Achillia fragrantissima(***)and Artemissia monosperma(***)were the most efficient as ion chelator(100%at 100,200 and 400μg/mL)*** and Rhanlarium appoposum(***.)showed 100%inhibition on peroxidation of linoleic acid emulsion at 200 and 400μg/mL,while butylatedhydroxy toluene and ascorinc acid showed 100and 95%inhibition percentage at 400μg/mL,*** various antioxidant activities were compared to standard antioxidants such as butylated hydroxyl toluene and ascorbic ***:In most tests *** and *** had powerful antioxidant properties as radical scavenger,reducing agent and superoxide anion radical scavenger.

关键词： Medicinal plants Antioxidant Polyphenol Saudi Arabia Asteraceae species

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The protection of Thymus vulgaris leaves alcoholic extract against hepatotoxicity of alcohol in rats

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Asian Pacific Journal of Tropical Medicine 2017年第4期10卷 341-351页

作者： Samah A.El-Newary Nermeen M.Shaffie E.A.Omer Medicinal and aromatic plants Research department Pharmaceutical Industries Research DivisionNational Research Center Pathology department Medical Researches DivisionNational Research CentreGiza Egypt

Objective:This study was performed to investigate the protective effect of Thymus vulgaris(***) leaves 70%alcoholic extract against alcohol-mediate hepatotoxicity ***:The protective effect of extract was investigated at dose of 500 mg/kg/day orally against alcohol-mediate hepatotoxicity using adult male Wister albino rats during 21 *** effect of *** extract was evaluated comparing with silymarin standard drug al recommended dose(25 mg/kg/day) orally for 21 ***:Alcohol-mediate hepatotoxicity rats(alcohol-control) showed hepatocytes distortion represented as marked increment on liver biomarkers;alkaline phosphatase(ALP),aspartate transaminase(AST) and alanine transaminase(ALT) activities,as well as pronounced reduction on total protein and its fractions albumin and globulin corresponding to normal *** to oxidative stress status as depletion on glutathione concentration,catalase(CAT),superoxide dismutase(SOD),glutathione reductase(GR),glutathione-S-transferase(GST) and glutathione peroxidase(GPx)activities,concurrence with augmentation oxidative stress parameters;malondyaldchydc(MDA) and hydrogen peroxide(H_2O_2) concentrations comparing to normal *** administration elevated total cholesterol(TC),low density lipoprotein cholesterol(LDL-C) and high density lipoprotein cholesterol(HDL-C) comparing to normal ***-administration *** extract with alcohol showed protective effect on hepatocytes manifested as minimizing on *** and ALT activities and increment on total protein,albumin and globulin production compared to *** enzymes activities;***,GST and GPx were significantly magnified,while MDA and H_2O_2 concentration were lessened corresponding to ***,lipid profile was markedly improved and risk ratio was lowered compared to *** results were confirmed by normalization of degenerated and fibrotic liver tissue as of alcohol-cont

关键词： Thymus vulgaris Alcohol-mediate hepatotoxicity Polyphenols Flavonoids Antioxidants enzymes activities Oxidative stress Histopathology

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