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Testing the polar auxin transport model with a selective plasma membrane H^(+)-ATPase inhibitor

Journal of Integrative Plant Biology 2022年第6期64卷 1229-1245页

作者： Yongqing Yang Xiaohui Liu Wei Guo Wei Liu Wei Shao Jun Zhao Junhong Li Qing Dong Liang Ma Qun He Yingzhang Li Jianyong Han Xiaoguang Lei State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China Beijing National Laboratory for Molecular sciences Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of EducationDepartment of Chemical BiologyCollege of Chemistry and Molecular EngineeringSynthetic and Functional Biomolecules Centerand Peking‐Tsinghua Center for Life SciencesPeking UniversityBeijing 100871China Institute of Food Science and Technology Chinese Academy of Agricultural SciencesBeijing 100193China department of Dermatology Peking University First HospitalBeijing 100034China Iomics Biosciences Inc. Beijing 100102China College of biological sciences China Agricultural UniversityBeijing 100193China

Auxin is unique among plant hormones in that its function requires polarized transport across plant cells.A chemiosmotic model was proposed to explain how polar auxin transport is derived by the H^(+)gradient across the plasma membrane(PM)established by PM H^(+)-adenosine triphosphatases(ATPases).However,a classical genetic approach by mutations in PM H^(+)-ATPase members did not result in the ablation of polar auxin distribution,possibly due to functional redundancy in this gene *** confirm the crucial role of PM H^(+)-ATPases in the polar auxin transport model,we employed a chemical genetic *** a chemical screen,we identified protonstatin-1(PS-1),a selective small-molecule inhibitor of PM H^(+)-ATPase activity that inhibits auxin *** with transgenic plants and yeast strains showed that the activity of PM H^(+)-ATPases affects auxin uptake as well as acropetal and basipetal polar auxin *** propose that PS-1 can be used as a tool to interrogate the function of PM H^(+)-*** results support the chemiosmotic model in which PM H^(+)-ATPase itself plays a fundamental role in polar auxin transport.

关键词： Arabidopsis auxin transport inhibitor plasma membrane H^(+)-ATPase

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Ale beer containing free and immobilized Lactobacillus brevis, a potential deliverysystem for probiotics

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Food Production, Processing and Nutrition 2021年第1期3卷 92-105页

作者： Kriza Faye Calumba Vondel Reyes Franklin Bonilla Elio Villasmil Subramaniam Sathivel School of Nutrition and Food sciences 220 H.D.Wilson LaboratoriesLouisiana State University Agricultural CenterBaton RougeLA 70803USA department of biological and agricultural engineering Louisiana State UniversityBaton RougeLA 70803USA

Probiotics in ale beer may be attractive to health-conscious ***,beer conditions may decrease probiotic *** produced from durian(Durio zibethinus)rind,a by-product that is currently unutilized,can be used for the immobilization of *** medium was incubated with Lactobacillus brevis and periodically sampled to obtain the growth *** beer with free *** and cells immobilized in durian rind powder was produced and separately assessed during storage at 21°C for 24 *** physico-chemical parameters of both beers did not differ *** rind powder conferred protection up to 12 days of storage with the immobilized cells in the beer having a significantly higher count than the free cells,which can be due to the acid detergent fiber content(19.67%).Free and immobilized cells remained viable with counts of 4.89 and 5.00 log CFU/mL of beer,respectively,at the end of the storage *** treatments had approximate counts of 5 log CFU/mL after 120 min in simulated gastric and intestinal *** predominant bacterial species present at the end of storage were *** and *** study suggests that ale beer could be a potential delivery system for free and immobilized probiotic *** is one of the few studies demonstrating the use of probiotic lactic acid bacteria in beer brewing.

关键词： Probiotics Beer Durian Viability

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Influence of colour changes and moisture content during banana drying on laser backscattering

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International Journal of agricultural and biological engineering 2010年第2期3卷 46-51页

作者： Giuseppe Romano Dimitrios Argyropoulos Klaus Gottschalk Emanuele Cerruto Joachim Müller Institute of agricultural engineering University of HohenheimStuttgartGermany Leibniz-Institute of agricultural engineering Potsdam-Bornim ATBGermany department of agricultural engineering University of Agricultural SciencesCataniaItaly

Pre-drying treatments are frequently employed to preserve fruit *** objective of this research was to monitor colour changes of banana during drying by laser backscattering and to determine the influence of the fruit discolouration on the light distribution into banana ***,to examine the influence of drying on the laser backscatter,the relationship between moisture content and relative laser area of banana slices was analyzed with different degrees of colour *** experiments were conducted at drying air temperature of 63℃with various pre-treatments like chilling,soaking in ascorbic/citric acid and dipping in distilled *** untreated sample was used as a control.A laser diode emitting at 670 nm with 3 mW power was used as light *** backscattering relative laser area was used as an indicator for the light absorption into the *** high result achieved on coefficient of determination R^(2)(>0.93)confirmed linear relationship between relative laser area and moisture *** with ascorbic acid gave the best prediction of the moisture content with the standard error of 5.7 and 8.8 for the estimated intercept and *** results showed a significant difference of lightness(L*values)during drying according to the different *** a result,colour degradation did not have a significant influence on the absorption of light at 670 nm wavelength.

关键词： musa x paradisiaca colour change banana drying pre-treatment relative laser area backscattering

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Octopaminergic neurons function in appetitive but not aversive olfactory learning and memory in Bactrocera dorsalis

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Insect Science 2022年第6期29卷 1747-1760页

作者： Jin-Xin Yu Qian Xiang Jia-Bao Qu Yan-Min Hui Tao Lin Xin-Nian Zeng Jia-Li Liu Guangdong engineering Research Center for Insect Behavior Regulation College of Plant ProtectionSouth China Agricultural UniversityGuangzhouGuangdongChina College of Life sciences Department of Biological ScienceShangrao Normal UniversityShangraoJiangxiChina

The biogenic amine octopamine(OA,invertebrate counterpart of nora-drenaline)plays critical roles in the regulation of olfactory ***,OA has been thought to mediate appetitive but not aversive learning in honeybees,fruit flies(Drosophila),and ***,this viewpoint has recently been challenged because OA activity through aβ-adrenergic-like receptor drives both appetitive and aversive ***,we explored the roles of OA neurons in olfactory learning and memory retrieval in Bactrocera *** trained flies to associate an orange odor with a sucrose reward or to associate methyl eugenol,a male lure,with N,N-diethyl-3-methyl benzoyl amide(DEET)*** then treated flies with OA receptor antagonists before appetitive or aversive conditioning and a memory retention *** of OA receptor antagonist mianserin or epinastine into the abdomen of flies led to impaired of appetitive learning and memory retention with a sucrose reward,while aversive learning and memory reten-tion with DEET punishment remained *** results suggest that the OA signaling participates in appetitive but not aversive learning and memory retrieval in *** through OA receptors.

关键词： appetitive aversive Bactrocera dorsalis learning and memory octopamine

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MEDICAL APPLICATIONS OF BIOPOLYESTERS POLYHYDROXYALKANOATES

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Chinese Journal of Polymer Science 2013年第5期31卷 719-736页

作者：陈国强汪洋 MOE Key Lab of Bioinformatics and Systems Biology Department of Biological Science and BiotechnologySchool of Life SciencesTsinghua-Peking Center for Life SciencesTsinghua University Center for Nano and Micro Mechanics Tsinghua University State Key Laboratory of Plant Physiology and Biochemistry National Plant Gene Research CentreCollege of Biological SciencesChina Agricultural University

Microbial polyhydroxyalkanoates （PHAs） are a family of biopolyesters produced by many wild type and engineered bacteria. PHAs have diverse structures accompanied by flexible thermal and mechanical properties. Combined with their in vitro biodegradation, cell and tissue compatibility, PHAs have been studied for medical applications, especially medical implants applications, including heart valve tissue engineering, vascular tissue engineering, bone tissue engineering, cartilage tissue engineering, nerve conduit tissue engineering as well as esophagus tissue engineering. Most studies have been conducted in the authors＇ lab in the past 20＋ years. Recently, mechanism on PHA promoted tissue regeneration was revealed to relate to cell responses to PHA biodegradation products and cell-material interactions mediated by microRNA. Very importantly, PHA implants were found not to cause carcinogenesis during long-term implantation. Thus, PHAs should have a bright future in biomedical areas.

关键词： PHB PHA Polyhydroxyalkanoates Tissue engineering Biomaterials Implants.

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Field study and regression modeling on soil water distribution with mulching and surface or subsurface drip irrigation systems

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International Journal of agricultural and biological engineering 2021年第2期14卷 142-150页

作者： Mohamed A.Mattar Ahmed A.Al-Othman Hosam O.Elansary Ahmed M.Elfeky Akram K.Alshami agricultural engineering department College of Food and Agriculture SciencesKing Saud UniversityRiyadh 11451Saudi Arabia Plant Production department College of Food and Agriculture SciencesKing Saud UniversityRiyadh 11451Saudi Arabia agricultural engineering Research Institute(AEnRI) Agricultural Research CentreGizaEgypt

The soil water status was investigated under soil surface mulching techniques and two drip line depths from the soil surface(DL).These techniques were black plastic film(BPF),palm tree waste(PTW),and no mulching(NM)as the control *** DL were 15 cm and 25 cm,with surface drip irrigation used as the *** results indicated that both the BPF and PTW mulching enhanced the soil water retention capacity and there was about 6%water saving in subsurface drip irrigation,compared with ***,the water savings at a DL of 25 cm were lower(15-20 mm)than those at a DL of 15 cm(19-24 mm),whereas surface drip irrigation consumed more *** distribution of soil water content(θv)for BPF and PTW were more useful than for ***,mulching the soil with PTW is recommended due to the lower costs and using a DL of 15 ***θv values were derived using multiple linear regression(MLR)and multiple nonlinear regression(MNLR)*** regression analysis revealed the superiority of the MLR over the MNLR model,which in the training and testing processes had coefficients of correlation of 0.86 and 0.88,root mean square errors of 0.37 and 0.35,and indices of agreement of 0.99 and 0.93,respectively,over the MNLR ***,DL and spacing from the drip line had a significant effect on the estimation of θv.

关键词： palm tree waste mulching plastic film mulching soil water distribution regression models

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Tests to evaluate the ecological impact of treated ballast water on three Chinese marine species

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Chinese Journal of Oceanology and Limnology 2014年第5期32卷 1105-1117页

作者：张亚楠王子羲蔡磊明蔡翔孙文俊马立青 Environmental engineering Institute Dalian Maritime University department of Mechanical engineering Tsinghua University Ecological Monitoring Training and Research Center Zhejiang Academy of Agricultural Sciences (ZAAS) Shanghai Academy of Environmental sciences department of Chemical and Biochemical engineering Western University

Ballast water has been a topic of concern for some time because of its potential to introduce invasive species to new habitats. To comply with the International Convention for the Control and Management of Ships' Ballast Water and Sediments, members of the International Maritime Organization(IMO) must equip their ships with on-board treatment systems to eliminate organism release with ballast water. There are many challenges associated with the implementation of this IMO guideline, one of which is the selection of species for testing the ecological impacts of the treated ballast water. In the United States, ballast water toxicity test methods have been defined by the United States Environmental Protection Agency. However, the test methods had not been finalized in China until the toxicity test methods for ballast water were established in 2008. The Chinese methods have been based on species from three trophic levels: Skeletonema costatum, Neomysis awatschensis, and Ctenogobius gymnauchen. All three species live in broad estuarine and open sea areas of China; they are sensitive to reference toxicants and acclimatize easily to different conditions. In this paper, the biological characteristics, test processes and statistical analysis methods are presented for the three species. Results indicate that the methods for evaluating these three organisms can be included in the ecological toxicity tests for treated ballast water in China.

关键词： treated ballast water ecological impact marine species toxicity test methods

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Soil Temperature and Maize Nitrogen Uptake Improvement Under Partial Root-Zone Drying Irrigation

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Pedosphere 2016年第6期26卷 872-886页

作者： Fatemeh KARANDISH Ali SHAHNAZARI Assistant Professor Water Engineering DepartmentUniversity of ZabolZabol 35856-98613(Iran) Water engineering department Sari Agricultural Sciences and Natural Resources UniversitySari 378(Iran)

Soil temperature is a major effective factor on the soil and plant biological *** can affect soil temperature and thereby induces a temperature effect on plant growth,which may result in an economic increase due to higher yield and plant nutrition.A ?eld experiment was carried out to investigate the effects of three irrigation strategies including full irrigation(FI),partial root-zone drying(PRD) and de?cit irrigation(DI) on soil temperature and the consequent results on the grain yield and N uptake of maize(Zea May L.).Soil temperature was measured by time domain re?ectometry(TDR) sensors during the 2010 growing *** treatments were applied from 55 to 107 d after *** PRD treatment caused soil temperature to be in a favorable domain for a longer period(for over 60% of the measuring dates) as a consequent result of water movement to deeper soil layers compared with the other treatments;the PRD treatment also reduced soil temperature at deeper soil depths to below the maximum favorable soil temperature for maize root growth,which resulted in deeper root penetration due to both water availability and favorable soil *** to the FI treatment,the PRD treatment increased root water uptake by 50% and caused no signi?cant reduction in total N uptake,while this was not observed in the DI treatment partially due to the negative temperature effect of DI on plant growth,which consequently affected the water and nutrient uptake.A longer vegetation period in the PRD treatment was observed due to higher leaf N concentrations and no signi?cant reduction in maize grain yield occurred in the PRD treatment,compared with those in the FI *** on the results,having 15.2% water saving during the whole growing season,the PRD irrigation would positively affect soil temperature and the water and nutrient uptake as a consequent,which thereby would prevent signi?cant reduction in maize grain yield.

关键词： full irrigation deficit irrigation grain yield irrigation strategy leaf N concentration root growth water saving water uptake

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Determination of threshing and separation unit performance of rosemary(Rosmarinus officinalis)plants

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International Journal of agricultural and biological engineering 2021年第6期14卷 237-243页

作者： Deniz Yilmaz Mehmet Emin Gökduman department of agricultural Machinery&Technology engineering Faculty of AgricultureIsparta University of Applied SciencesIspartaTurkey

Rosemary(Rosmarinus officinalis L.)is an important perennial medicinal and aromatic plant belonging to the Lamiaceae *** is widely produced in Turkey for its oil which can be extracted from the plants when flowers are in *** Turkey,a significant amount of domestic and foreign trade of rosemary is *** 2018-2020,2088 t of rosemary plants were imported,while in the same years 2077 t were *** this study,the performance indexes of a threshing and separating unit designed according to the physicomechanical properties of the rosemary were *** operating parameters of the machine were determined at three different moisture contents and adjusted according to the harvest moisture *** threshing efficiency,work efficiency,power requirement,and specific energy consumption values of the threshing unit were determined,while separating efficiency,work efficiency and specific power consumption of separating unit of the developed system were *** to the study results,the threshing efficiency of the rosemary plant varied between 44.00%and 96.97%.Work efficiency of the thresher varied between 1.18 and 15.34 kg/*** requirements and specific energy consumption of the threshing unit were obtained as 0.19-1.05 kW and 0.02-0.37 kW·h/h,*** the other hand,the separating efficiency of the separating unit for the rosemary plant varied between 44.27%and 85.24%.Maximum and minimum values of work efficiency were found as 0.75 kg/h and 5.52 kg/*** power consumption of the unit ranged from 3.95 to 39.95 kW/kg.

关键词： rosemary mechanization threshing separating design Turkey

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Estimation of citrus canker lesion size using hyperspectral reflectance imaging

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International Journal of agricultural and biological engineering 2013年第3期6卷 41-51页

作者： Nikhil P.Niphadkar Thomas F.Burks Jianwei Qin Mark A.Ritenour ATW Automation Inc. DaytonOH 45402USA department of agricultural and biological engineering University of FloridaGainesvilleFL 32611USA U.S.department of Agriculture BeltsvilleMD 20705USA department of Horticultural sciences University of FloridaGainesvilleFL 34945USA

The Citrus industry has need for effective approaches to remove fruit with canker before they are shipped to selective international market such as the European *** research aims to determine the detectable size limit for cankerous lesions using hyperspectral imaging *** developed multispectral algorithms using visible to near-infrared wavelengths,were used to segregate cankerous citrus fruits from other peel conditions(normal,greasy spot,insect damage,melanose,scab and wind scar).However,this previous work did not consider lesion size.A two-band ratio method with a simple threshold based classifier(ratio of reflectance at wavelengths 834 nm and 729 nm),which gave maximum overall classification accuracy of 95.7%,was selected for lesion size estimation in this *** smallest size of cankerous lesion detected in terms of equivalent diameter was 1.66 *** effect of variation of threshold values and number of erosion cycles(applying morphological erosion multiple times to the image)on estimation of smallest detectable lesion was *** was found that small threshold values gave better canker classification accuracies,while exhibiting a lower overall classification ***,higher threshold values portrayed the opposite *** threshold value of 1.275 gave the optimum tradeoff between canker classification accuracy,overall classification accuracy and minimal lesion size *** the number of erosion cycles reduced detection rates of smaller canker lesions,leading to the conclusion that a single erosion cycle gave the best size estimation *** erosion kernel of the size 3 mm×3 mm was used during the exploration.

关键词： citrus canker lesion size disease detection hyperspectral reflectance imaging image classification multispectral algorithm size detection limit

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