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Expressed sequence tag analysis and cloning of trehalose-6-phosphate synthase gene from marine alga Laminaria japonica(Phaeophyta)

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Acta Oceanologica Sinica 2012年第6期31卷 139-148页

作者： XUAN Jinsong 1,4,FENG Yanbin 1,WENG Manli 1,ZHAO Ge 1,SHI Jinfeng 1,YAO Jianting 2,WANG Xiuliang 2,GUO Baotai 3,QIAO Lixian 3,DUAN Delin 2,WANG Bin 1 1 The State Key Laboratory of Plant Genomics,Institute of Genetics and Developmental Biology,Chinese Academy of sciences,Beijing 100101,China 2 The State Key Laboratory of Marine Biology,Institute of Oceanology,Chinese Academy of sciences,Qingdao 266071,China 3 College of Life sciences,Qingdao agricultural University,Qingdao 266109,China 4 department of biological Science and engineering,School of Chemical and biological engineering,University of Science and Technology Beijing,Beijing 100083,China The State Key Laboratory of Plant Genomics Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing China department of biological Science and engineering School of Chemical and Biological Engineering University of Science and Technology Beijing Beijing China The State Key Laboratory of Marine Biology Institute of Oceanology Chinese Academy of Sciences Qingdao China College of Life sciences Qingdao Agricultural University Qingdao China

A high quality cDNA library was constructed from the brown alga Laminaria japonica,with the titer of 1.2×10 5 pfu/*** average insert size of the cDNA library is about 1.6 *** the cDNA library,591 cDNA clones were randomly selected and *** a result,574 EST(expressed sequence tag) sequences were *** of the 574 ESTs were submitted to the dbEST database section of GenBank with the accession numbers from CX942625 to *** cDNA library was screened with a α-32 p labeled 453 bp T P S gene probe,which is a partial sequence yielded from Porphyra *** positive cDNA clones were screened and the sequencing data showed that these four cDNA clones covered majority of *** TPS cDNA *** PCR amplification,sequencing and assembling,the entire ORF(open reading frame) sequence of the T P S gene was obtained,which was named *** encodes a protein containing 908 amino acids with a calculated molecular mass of 101 674 *** LjTPS gene was successfully expressed in *** and *** LjTPS gene has potential application both in plant breeding to stress tolerance and in deciphering the T P S gene function and mechanism to stress tolerance.

关键词： cDNA library comparative study expressed sequence tag(EST) analysis Laminaria japonica trehalose-6-phosphate synthase(TPS) T P S gene

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Poverty alleviation and resource conservation through development of cost effective technology at foot hill of Shivalik - A case study

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International Journal of agricultural and biological engineering 2013年第3期6卷 34-40页

作者： R Kumar P K Singh B A Langoo Division of agricultural engineering Sher-e-Kashmir Univeristy of Agricultural Sciences and Techonology of KashmirSrinagar(J&K)India department of soil and water engineering College of Technology and EngineeringUdaipur(Raj)India

The study revealed the development of cost effective technology utilized as a practical tool for treatment of seasonal torrent and addressing erosion problems and land use *** was implemented in a small agricultural watershed located in foot hill of Shivalik,India,to assess its prediction capacity of runoff,peak runoff flow and sediment *** effective technology was evaluated at the event scale by using a database of hydrological,geomorphologic and land use data collected during a two-year *** the catchment,the gullies which are small to medium in size were treated with gully plugging by erecting loose boulder check dams and erected with different species of *** types of spur were constructed with vegetative reinforcement for channelization of stream *** sediments deposited in the first year at downstream was recorded 0.09-81.0 tons,while in the second year it was reduced up to 0.07-16.7 ***,up stream sediment deposition was recorded 1.0-72.0 tons and 0.37-13.1 tons in two consecutive *** D-50 analysis of sediment deposited was carried in three different places of torrent and it was found that deposited particle size material decreased after treatment undertaken in the torrent ***,the mechanical as well as vegetative measures helped in the channelization of water course towards the central line with the tune of 10-100 m,stabilization of torrent bed and reclamation of degraded land.

关键词： Integrated watershed management mechanical measures D-50 vegetative measures

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Modification of colorimetric method based digital soil test kit for determination of macronutrients in oil palm plantation

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International Journal of agricultural and biological engineering 2020年第4期13卷 188-197页

作者： Muhammad Yamin Wan Ishak bin Wan Ismail Muhamad Saufi bin Mohd Kassim Samsuzana Binti Abd Aziz Farah Naz Akbar Redmond R.Shamshiri Muhammad Ibrahim Benjamin Mahns department of Farm Machinery&Power Faculty of Agricultural Engineering&TechnologyUniversity of AgricultureFaisalabad(38040)Pakistan department of biological and agricultural engineering Faculty of EngineeringUniversiti Putra Malaysia43400 UPM SerdangSelangorMalaysia department of Allied Health sciences Sargodha Medical CollegeUniversity of Sargodha(40100)Pakistan Leibniz Institute for agricultural engineering and Bioeconomy Max-Eyth-Allee 10014469 Potsdam-BornimGermany department of Environmental sciences&engineering Government College University Faisalabad(38000)Pakistan

It is the need of time that oil palm farmers must perform the spatially planned soil analysis to know about the fertilizer sufficient and deficient zones of *** method is a suitable and fast solution of soil analysis for NPK determination using the digital soil test *** determination procedure with a digital soil test kit was undefined for oil ***,the digital soil test kit determines the passage of light through an opaque medium of soil solution with a specified ***,environmental light may interfere leading to wrong results of NPK ***,this equipment was non-incorporable with the controller of any VRT fertilizer *** this research,these issues were addressed and the NPK measurement procedure was defined for oil palm plantation by modifying the‘soil to water’ratio in sample soil solution with an optimum environmental light range of 18-23 W/m^(2).‘Soil to water’ratios were found for nitrogen,phosphorus and potassium as 0.31 to 5.00,1.00 to 5.00 and 4.50 to 5.00,respectively to fit the requirement of NPK for oil palm in the prescribed range of the *** study of modified digital soil test kit showed that 91.7%N,89.6%P and 93.8%K results of modified digital soil test kit were matched with analytical laboratory ***,the reliability of NPK results using digital soil test kit was enhanced,making the kit incorporable with the controller of variable rate fertilizer applicator through remote monitoring based data acquisition *** outcome of this research can be used in the development of an IoT network data fusion for dynamic assessment of the NPK variation in the soil and nutrient management in oil palm plantations.

关键词： digital soil test kit variable rate fertilizer applicator oil palm NPK measurement data acquisition system colorimetric method

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Moisture dependent physical properties of Lagenaria siceraria seed

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International Journal of agricultural and biological engineering 2013年第4期6卷 111-120页

作者： Prashant Pandharinath Said Rama Chandra Pradhan department of Farm engineering Institute of Agricultural SciencesBanaras Hindu UniversityVaranasiUttar Pradesh 221005India

The investigation was done to evaluate the effect of moisture content on some physical properties of Lagenaria siceraria *** study was conducted at five moisture levels including 8.84%,10.10%,11.89%,12.60%and 15.10%(w.b.).Results showed that the mean value of principal dimensions,average diameters,surface area and 1000 seed mass increased linearly but aspect ratio decreased with increase in moisture *** sphericity increased in the moisture range of 8.84%to 11.89%but decreased with further raise in the moisture up to 15.10%.Gravimetric properties like bulk density increased with increase in moisture content however true density and porosity decreased linearly with increase in moisture *** of repose and terminal velocity increased linearly with moisture content of the *** coefficient of friction increased linearly with seed moisture content on five experimental surfaces(plywood,galvanized iron,glass and plastic).The information pertaining to moisture dependant physical properties of Lagenaria siceraria seeds may become an essential part in design of processing machines and its unit operations,design of dehulling,oil expression and other processing equipments.

关键词： Lagenaria siceraria physical properties angle of repose aspect ratio gravimetric properties sphericity

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Evaluation of the Efficacy of an Aloe barbadensis Based biological Insecticide against Pests of Abelmochus esculentus for Promoting Ecological Agriculture (Far-North, Cameroon)

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agricultural sciences 2024年第5期15卷 590-603页

作者： Mba Félicité Obono Laurentine Naoutissa Samuel Ntamack Augustin Goudoum department of Environmental Science National Advanced School of Engineering The University of Maroua Maroua Cameroon department of Agriculture Livestock and Derived Products National Advanced School of Engineering The University of Maroua Maroua Cameroon

Chemical insecticides have been considered as a means to combat crop pests. Although their effectiveness is evident, their impact on the environment is increasingly being discussed. The aim of this study is to determine the agro-ecological potential of a biological insecticide (C25H32O12) based on Aloe barbadensis in a Sahelian context. For this purpose, a completely randomized block experimental design with 3 replications and 4 treatments was set up to experiment with Aloe barbadensis as a bioinsecticide against pests of Abelmoschus esculentus. However, data were collected using an observation and parameter monitoring grid. This includes the cultivation of Abelmoschus esculentus, soil preparation, seeding and watering, plot labeling, preparation of the bioinsecticide (selection and preparation of raw materials, grinding of Aloe barbadensis miller and extraction of the crude bioinsecticide, quantification of treatment doses and dilution, and obtaining the formulated bioinsecticide), plant watering, plant treatment, and finally parameter monitoring. The results obtained reveal that the level of damage is significantly high in the control treatment T0 (63%) compared to the other treatments, with 29% for treatment T1, 7% for T2, and 1% for T3, implying a strong action capability of this insecticide against pests of Abelmoschus esculentus. Therefore, it can be concluded that for a normal growing season of Abelmoschus esculentus, this biological insecticide should be sprayed 12 times. Furthermore, this biological insecticide is unique in that it does not inflict any gastric toxicity on the pests, which gives it the characteristic of being a repellent. It is a biological insecticide whose efficacy period has been tested, with a minimum duration of 21 days. In conclusion, this formulated bioinsecticide based on Aloe barbadensis demonstrates significant efficacy against pests of Abelmoschus esculentus. In the future, we will consider experim

关键词： Aloe biological Insecticide Pests Agro-Ecological

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Bio-Adaption between Magnesium Alloy Stent and the Blood Vessel:A Review

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Journal of Materials Science & Technology 2016年第9期32卷 815-826页

作者： Jun Ma department of Chemical Biological and BioengineeringNorth Carolina Agricultural and Technical State UniversityGreensboroNCUSA NSF engineering Research Center-Revolutionizing Metallic Biomaterials North Carolina Agricultural and Technical State UniversityGreensboroNC USA

Biodegradable magnesium （Mg） alloy stents are the most promising next generation of bio-absorbable stents. In this article, we summarized the progresses on the in vitro studies, animal testing and clinical trials of biodegradable Mg alloy stents in the past decades. These exciting findings led us to propose the importance of the concept ＂bio-adaption＂ between the Mg alloy stent and the local tissue microenvironment after implantation. The healing responses of stented blood vessel can be generally described in three overlapping phases： inflammation, granulation and remodeling. The ideal bio-adaption of the Mg alloy stent, once implanted into the blood vessel, needs to be a reasonable function of the time and the space/dimension. First, a very slow degeneration of mechanical support is expected in the initial four months in order to provide sufficient mechanical support to the injured vessels. Although it is still arguable whether full mechanical support in stented lesions is mandatory during the first four months after implantation, it would certainly be a safety design parameter and a benchmark for regulatory evaluations based on the fact that there is insufficient human in vivo data available, especially the vessel wall mechanical properties during the healing/remodeling phase. Second, once the Mg alloy stent being degraded, the void space will be filled by the regenerated blood vessel tissues. The degradation of the Mg alloy stent should be 100% completed with no residues, and the degradation products （e.g., ions and hydrogen） will be helpful for the tissue reconstruction of the blood vessel. Toward this target, some future research perspectives are also discussed.

关键词： Mg stent Bio-adaptation Vessel Wound healing

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Structure and activity of thermophilic methanogenic microbial communities exposed to quaternary ammonium sanitizer

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Journal of Environmental sciences 2017年第6期29卷 164-168页

作者： Jesus D.Fernandez-Bayo Juliano Toniato Blake A.Simmons Christopher W.Simmons department of Food Science and Technology University of CaliforniaDauisCA 95616USA department of biological and agricultural engineering Uniuersity of CaliforniaDauisCA 95616USA Joint BioEnergy Institute EmeryvilleCA 94608USA biological and engineering sciences Center Sandia National LaboratoriesLiuermoreCA 94551USA Energy Efficiency Center University of CaliforniaDauisCA 95616USA

Food processing facilities often use antimicrobial quaternary ammonium compound （QAC） sanitizers to maintain cleanliness. These QACs can end up in wastewaters used as feedstock for anaerobic digestion. The aim of this study was to measure the effect of QAC contamination on biogas production and structure of microbial communities in thermo- philic digester sludge. Methane production and biogas quality data were analyzed in batch anaerobic digesters containing QAC at 0, 15, 50, 100 and 150 mg/L. Increasing sanitizer concentration in the bioreactors negatively impacted methane production rate and biogas quality. Microbial community composition data was obtained through 16S rRNA gene sequencing from the QAG-contaminated sludges. Sequencing data showed no significant restructuring of the bacterial communities. However, significant restructuring was observed within the archaeal communities as QAC concentration increased. Further studies to confirm these effects on a larger scale and with a longer retention time are necessary.

关键词： Anaerobic digestionAntimicrobialsBiofuelsMicrobial ecologyWaste management

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Semi-synthesis of Ubiquitin-propargylamide for identifying deubiquitinase targeting inhibitors

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Chinese Chemical Letters 2019年第3期30卷 743-746页

作者： Xiaodan Tan Jingsi Bai Shan Ding Yujing Ren Danning Hong Ziqing Mei Yi-Ming Li School of biological and Medical engineering Hefei University of Technology Tsinghua-Peking Center for Life sciences Ministry of Education Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical BiologyDepartment of Chemistry Tsinghua University Biotechnology Research Institute Chinese Academy of Agricultural Sciences School of biological Science Beijing Institute of Technology

Ubiquitin-propargylamide(Ub-PA) is one of the most widely used activity-based probe to measure the activity of deubiquitinases(DUBs) and help validate DUBs targeting inhibitors. However, current synthetic route of Ub-PA is cumbersome. In this work, we report a novel semi-synthetic strategy to prepare Ub-PA in large-scale. Biochemical assays prove that semi-synthetic Ub-PA is an effective probe in identifying DUBs targeting inhibitors.

关键词： Deubiquitinase Activity-based probe Ubiquitin-propargylamide Protein semi-synthesis Inhibitor identification

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Real-time monitoring of optimum timing for harvesting fresh tea leaves based on machine vision

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International Journal of agricultural and biological engineering 2019年第1期12卷 6-9页

作者： Liang Zhang Hongduo Zhang Yedong Chen Sihui Dai Xumeng Li Kenji Imou Zhonghua Liu Ming Li College of engineering Hunan Agricultural UniversityChangsha 410128China College of Horticulture and Landscape Hunan Agricultural UniversityChangsha 410128China agricultural College Hunan Agricultural UniversityChangsha 410128China department of biological and Environmental engineering Graduate School of Agricultural and Life SciencesUniversity of TokyoTokyo 113-0033Japan School of agricultural engineering and Food Science Shandong University of TechnologyZibo 255000China Hunan agricultural Aviation Advanced Technology engineering Research Center Changsha 410129China

The harvesting time of fresh tea leaves has a significant impact on product yield and *** aim of this study was to propose a method for real-time monitoring of the optimum harvesting time for picking fresh tea leaves based on machine ***,the shapes of fresh tea leaves were distinguished from RGB images of the tea-tree canopy after graying with the improved B-G algorithm,filtering with a median filter algorithm,binary processing with the Otsu algorithm,and noise reduction and edge smoothing using open and close *** the leaf characteristics,such as leaf area index,average length,and leaf identification index,were *** on these,the Bayesian discriminant principle and method were used to construct a discriminant model for fresh tea-leaf collection *** this method was applied to a RGB tea-tree canopy image acquired at 45°shooting angle,the fresh tea-leaf recognition rate was 90.3%,and the accuracy for fresh tea-leaf harvesting status was 98%by cross ***,this method provides the basic conditions for future tea-plantation operation and management using information technology,automation,and intelligent systems.

关键词： agricultural machinery fresh tea leaves machine vision intelligent recognition real-time monitoring

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Effect of smart sprinkler irrigation utilization on water use efficiency for wheat crops in arid regions

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International Journal of agricultural and biological engineering 2014年第1期7卷 26-35页

作者： Hussein Mohammed Al-Ghobari Mohamed Said Abdalla El Marazky department of agricultural engineering College of Food and Agriculture Sciences King Saud UniversityRiyadh 11451Kingdom of Saudi Arabia

The smart irrigation system(SIS)developed in this research is a valuable tool for scheduling irrigation and quantifying water required by *** was implemented and tested under sprinkler irrigation system to irrigate wheat crops(YecoraRojo).Results obtained from this system were compared with the control irrigation system(CIS),whose scheduling method was based on data from an automatic weather *** indicated significant savings in applied water using the *** addition,the use of the SIS conserved 12%of irrigation water compared to CIS and obtained an economical *** water use efficiency(WUE)under SIS had generally higher values(1.64 kg/m^(3))compared to CIS(1.46 kg/m^(3)).Hence,the application of SIS technology provides significant advantages on WUE and irrigation water use efficiency(IWUE).Relatively high WUE and IWUE were found for the irrigation treatment(80%of evapotranspiration under SIS).Results showed that the irrigation requirements of wheat increased(100%of ETc under CIS)with increasing evapotranspiration(ETc)but excessive irrigation could decrease WUE and *** results indicated that extreme irrigation might not produce higher yield or optimal economic benefit,thus,suitable irrigation schedules by using SIS must be established and extendable to other agricultural crops.

关键词： smart irrigation system(SIS) sprinkler irrigation scheduling water use efficiency arid region evapotranspiration grain yield

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