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SLAF marker based QTL mapping of fruit-related traits reveals a major-effect candidate locus ff2.1 for flesh firmness in melon

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Journal of Integrative Agriculture 2023年第11期22卷 3331-3345页

作者： CHEN Ke-xin DAI Dong-yang WANG Ling YANG Li-min LI Dan-dan WANG Chao JI Peng SHENG Yun-yan Department of Horticulture and Landscape Architecture Heilongjiang Bayi Agricultural UniversityDaqing 163309P.R.China

Flesh firmness(FF) is an important and complex trait for melon breeders and consumers. However, the genetic mechanism underlying FF is unclear. Here, a soft fruit melon(P5) and a hard fruit melon(P10) were crossed to generate F2, and the FF and fruit-related traits were recorded for two years. By performing quantitative trait locus(QTL) specificlocus amplified fragment(SLAF)(QTL-SLAF) sequencing and molecular marker-linkage analysis, 112 844 SLAF markers were identified, and 5 919 SNPs were used to construct a genetic linkage map with a total genetic distance of1 356.49 cM. Ten FF-and fruit-related QTLs were identified. Consistent QTLs were detected for fruit length(FL) and fruit diameter(FD) in both years, and QTLs for single fruit weight(SFW) were detected on two separate chromosomes in both years. For FF, the consistent major locus(ff2.1) was located in a 0.17-Mb candidate region on chromosome 2. Using 429 F2individuals derived from a cross between P5 and P10, we refined the ff2.1 locus to a 28.3-kb region harboring three functional genes. These results provide not only a new candidate QTL for melon FF breeding but also a theoretical foundation for research on the mechanism underlying melon gene function.

关键词： QTL mapping flesh firmness fruit-related traits melon SLAF sequencing

Phosphate Starvation Responses and Gibberellic Acid Biosynthesis Are Regulated by the MYB62 Transcription Factor in Arabidopsis

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Molecular Plant 2009年第1期2卷 43-58页

作者： Ballachanda N. Devaiah Ramaiah Madhuvanthi Athikkattuvalasu S. Karthikeyan Kashchandra G. Raghothama Department of Horticulture and Landscape Architecture Purdue University West Lafayette IN 47907-116S USA

The limited availability of phosphate （Pi） in most soils results in the manifestation of Pi starvation responses in plants. To dissect the transcriptional regulation of Pi stress-response mechanisms, we have characterized the biological role of MYB62, an R2R3-type MYB transcription factor that is induced in response to Pi deficiency. The induction of MYB62 is a specific response in the leaves during Pi deprivation. The MYB62 protein localizes to the nucleus. The overexpression of MYB62 resulted in altered root architecture, Pi uptake, and acid phosphatase activity, leading to decreased total Pi content in the shoots. The expression of several Pi starvation-induced （PSI） genes was also suppressed in the MYB62 overexpressing plants. Overexpression of MYB62 resulted in a characteristic gibberellic acid （GA）-deficient phenotype that could be partially reversed by exogenous application of GA. In addition, the expression of SOC1 and SUPERMAN, molecular reg- ulators of flowering, was suppressed in the MYB62 overexpressing plants. Interestingly, the expression of these genes was also reduced during Pi deprivation in wild-type plants, suggesting a role for GA biosynthetic and floral regulatory genes in Pi starvation responses. Thus, this study highlights the role of MYB62 in the regulation of phosphate starvation responses via changes in GA metabolism and signaling. Such cross-talk between Pi homeostasis and GA might have broader implications on flowering, root development and adaptive mechanisms during nutrient stress.

关键词： Abiotic/environmental stress nutrition signal transduction.

Evaluation of ambient mass spectrometry tools for assessing inherent postharvest pepper quality

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horticulture Research 2021年第1期8卷 2252-2259页

作者： Tyler J.Mason Harmonie M.Bettenhausen Jacqueline M.Chaparro Mark E.Uchanski Jessica E.Prenni Department of Horticulture and Landscape Architecture Colorado State UniversityFort CollinsCO 80524USA

Horticulturists are interested in evaluating how cultivar,environment,or production system inputs can affect postharvest quality.Ambient mass spectrometry approaches enable analysis of minimally processed samples under ambient conditions and offer an attractive high-throughput alternative for assessing quality characteristics in plant products.Here,we evaluate direct analysis in real time(DART-MS)mass spectrometry and rapid evaporative ionization-mass spectrometry(REIMS)to assess quality characteristics in various pepper(Capsicum annuum L.)cultivars.DART-MS exhibited the ability to discriminate between pod colors and pungency based on chemical fingerprints,while REIMS could distinguish pepper market class(e.g.,bell,lunchbox,and popper).Furthermore,DART-MS analysis resulted in the putative detection of important bioactive compounds in human diet such as vitamin C,p-coumaric acid,and capsaicin.The results of this study demonstrate the potential for these approaches as accessible and reliable tools for high throughput screening of pepper quality.

关键词： cultivar pepper ambient

Protecting genes from RNA silencing by destroying aberrant transcripts

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Science China(Life Sciences) 2015年第6期58卷 613-615页

作者： ZHANG Heng ZHU JianKang Shanghai Center for Plant Stress Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Department of Horticulture and Landscape Architecture Purdue University

Gene expression is intensively regulated at the transcript level.It is the rate of transcription and the rate of degradation that together determine the steady-state level of a specific transcript.A whole-genome study indicates that the half-life of Arabidopsis transcripts varies from 0.2 to>24 h,indicating large variations in the turnover rate of specific transcripts,which is related to their biological functions[1].

关键词：转录水平 RNA沉默基因保护异常基因表达调控生物学功能降解作用全基因组

New discoveries generate new questions about RNA-directed DNA methylation in Arabidopsis

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National Science Review 2017年第1期4卷 10-15页

作者： Huiming Zhang Jian-Kang Zhu Shanghai Center for Plant Stress Biology and CAS Center for Excellence in Molecular Plant SciencesChinese Academy of Sciences Department of Horticulture and Landscape Architecture Purdue University

Plants can establish de novo DNA methylation via the RNA-directed DNA methylation(RdDM)pathway,which involves a complex array of proteins as well as small interfering RNAs(siRNAs)and scafold RNAs.In the canonicalRd DM pathway that is best understood in Arabidopsis,complementary pairing between 24-nt siRNAs and nascent scafold RNAs,together with protein–protein interactions,recruits the protein machinery for DNA methylation.

关键词： DCL RNA DNAmethylation AGO

A group of SUVH methyl-DNA binding proteins regulate expression of the DNA demethylase ROS_1 in Arabidopsis

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Journal of Integrative Plant Biology 2019年第2期61卷 110-119页

作者： Xinlong Xiao Jieqiong Zhang Tao Li Xing Fu Viswanathan Satheesh Qingfeng Niu Zhaobo Lang Jian-Kang Zhu Mingguang Lei Shanghai Center for Plant Stress Biology Center for Excellence in Molecular Plant SciencesChinese Academy of Sciences The University of Chinese Academy of Sciences Department of Horticulture and Landscape Architecture Purdue University

DNA methylation is typically regarded as a repressive epigenetic marker for gene expression.Genome-wide DNA methylation patterns in plants are dynamically regulated by the opposing activities of DNA methylation and demethylation reactions. In Arabidopsis, a DNA methylation monitoring sequence(MEMS) in the promoter of the DNA demethylase gene ROS_1 functions as a methylstat that senses these opposing activities and regulates genome DNA methylation levels by adjusting ROS_1 expression. How DNA methylation in the MEMS region promotes ROS_1 expression is not known. Here, we show that several Su(var)3-9 homologs(SUVHs) can sense DNA methylation levels at the MEMS region and function redundantly to promote ROS_1 expression. The SUVHs bind to the MEMS region, and the extent of binding is correlated with the methylation level of the MEMS.Mutations in the SUVHs lead to decreased ROS_1 expression, causing DNA hypermethylation at more than 1,000 genomic regions. Thus, the SUVHs function to mediate the activation of gene transcription by DNA methylation.

关键词： DNA ROS Figure

OST1 phosphorylates ICE1 to enhance plant cold tolerance

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Science China(Life Sciences) 2015年第3期58卷 317-318页

作者： LANG ZhaoBo ZHU JianKang Shanghai Center for Plant Stress Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Department of Horticulture and Landscape Architecture Purdue University

Low temperature is one of the most important environmental stresses that affect plant survival,growth and development.In response to cold stress,hundreds of genes are upor down-regulated in plants[1].The C-repeat binding factor(CBF)regulon is the best-understood cold-responsive transcriptional module[1].In Arabidopsis,there are three CBF genes,i.e.,CBF1,CBF2 and CBF3.They are rapidly in-

关键词：抗寒能力 CBF基因磷酸植株植物生存环境压力结合因子 CBF1

Maintenance of water uptake and reduced water loss contributeto water stress tolerance of Spiraea alba Du Roi and Spiraeatomentosa L.

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horticulture Research 2014年第1期1卷 150-156页

作者： Kelly M Stanton Michael V Mickelbart Department of Horticulture and Landscape Architecture Purdue UniversityWest LafayetteIN 47907USA

Two primarily eastern US native shrubs,Spiraea alba Du Roi and Spiraea tomentosa L.,are typically found growing in wet areas,often with standing water.Both species have potential for use in the landscape,but little is known of their environmental requirements,including their adaptation to water stress.Two geographic accessions of each species were evaluated for their response to water stress under greenhouse conditions.Above-ground biomass,water relations and gas exchange were measured in well-watered and water stress treatments.In both species,water stress resulted in reduced growth,transpiration and pre-dawn water potential.However,both species also exhibited the ability to osmotically adjust to lower soil water content,resulting in maintained midday leaf turgor potential in all *** CO_(2) assimilation was reduced only in one accession of S.alba,primarily due to large reductions in stomatal conductance.S.tomentosa lost a larger proportion of leaves than S.alba in response to water stress.The primary water stress tolerance strategies of S.alba and S.tomentosa appear to be the maintenance of water uptake and reduced water loss.

关键词： stress primarily landscape

Brassinosteroids fine-tune secondary and primary sulfur metabolism through BZR1-mediated transcriptional regulation

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Journal of Integrative Plant Biology 2023年第5期65卷 1153-1169页

作者： Mengyu Wang Congxi Cai Yubo Li Han Tao Fanliang Meng Bo Sun Huiying Miao Qiaomei Wang department of horticulture Key Laboratory of Horticultural Plant GrowthDevelopment and Quality improvementMinistry of AgricultureZhejiang UniversityHangzhou 310058China School of horticulture and landscape architecture Yangzhou UniversityYangzhou 221116China Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province Zhejiang Academy of Agricultural SciencesHangzhou 310021China College of horticulture Sichuan Agricultural UniversityChengdu 611130China

For adaptation to ever-changing environments,plants have evolved elaborate metabolic systems coupled to a regulatory network for optimal growth and defense. Regulation of plant secondary metabolic pathways such as glucosinolates(GSLs) by defense phytohormones in response to different stresses and nutrient deficiency has been intensively investigated, while how growth-promoting hormone balances plant secondary and primary metabolism has been largely unexplored. Here, we found that growth-promoting hormone brassinosteroid(BR) inhibits GSLs accumulation while enhancing biosynthesis of primary sulfur metabolites, including cysteine(Cys) and glutathione(GSH) both in Arabidopsis and Brassica crops, fine-tuning secondary and primary sulfur metabolism to promote plant growth. Furthermore, we demonstrate that of BRASSINAZOLE RESISTANT 1(BZR1), the central component of BR signaling, exerts distinct transcriptional inhibition regulation on indolic and aliphatic GSL via direct MYB51 dependent repression of indolic GSL biosynthesis, while exerting partial MYB29 dependent repression of aliphatic GSL biosynthesis. Additionally, BZR1 directly activates the transcription of APR1 and APR2 which encodes rate-limiting enzyme adenosine 5′-phosphosulfate reductases in the primary sulfur metabolic pathway.In summary, our findings indicate that BR inhibits the biosynthesis of GSLs to prioritize sulfur usage for primary metabolites under normal growth conditions.These findings expand our understanding of BR promoting plant growth from a metabolism perspective.

关键词： brassinosteroids BZR1 glucosinolates plant growth primary sulfur metabolites secondary sulfur metabolites