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Berberine Inhibits Norepinephrine-Induced Apoptosis in Neonatal Rat Cardiomyocytes via Inhibiting ROS-TNF-α-Caspase Signaling Pathway

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Chinese Journal of Integrative Medicine 2013年第6期19卷 424-431页

作者：吕秀秀余小慧王华东严玉霞王彦平陆大祥戚仁斌胡巢凤李红梅 department of Pathophysiology Key Laboratory of State Administration of Traditional Chinese MedicineSchool of MedicineJinan University Institute of Integrative Medicine Jinan University department of Biochemistry School of MedicineJinan University

Objective： TO determine the effect of berberine （Ber） on norepinephrine （NE）-induced apoptosis in neonatal rat cardiomyocytes. Methods： The cultured neonatal rat cardiomyocytes were treated with NE in the presence or absence of Ber. The activity of lactate dehydrogenase （LDH） in the culture medium was examined, and apoptosis of cardiomyocytes was assessed by Hoechst 33258, isothiocyanate （FITC）- conjugated annexin-V, and propidine iodide （PI） staining. In addition, the activities of caspases-2 and-3 were measured by a fluorescent assay kit. The level of secreted tumor necrosis factor oL （TNF-a） and production of intracellular reactive oxygen species （ROS） were also determined. Results： NE at a concentration of 50 p, mol/L induced an obvious increase in the activity of LDH in the culture medium （P〈0.05）, which was inhibited by coincubation with 0.5, 1.0, or 2.0 μ mol/L Ber （P〈0.05）. Ber also significantly attenuated NE- induced apoptosis in a dose-dependent manner （P〈0.01）. Moreover, Ber at a dose of 2 μ mol/L markedly decreased the ROS and TNF-a productions （P〈0.05） and inhibited the activation of caspases-2 and -3 in cardiomyocytes exposed to NE （P〈0.05）. Conclusion： The present study suggested that Ber could reduce NE-induced apoptosis in neonatal rat cardiomyocytes through inhibiting the ROS-TNF-a-caspase signaling pathway.

关键词： apoptosis cardiomyocytes berberine norepinephrine

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Zero-waste emission design of sustainable and programmable actuators

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SusMat 2023年第2期3卷 207-221页

作者： Linchao Sun Lixuan Che Ming Li William George Neal Xu Leng Yaojia Long Yifeng Jia Yunhu Gao Matteo Palma Yao Lu department of Chemistry School of Physical and Chemical SciencesQueen Mary University of LondonLondonUK State Key Laboratory of Structural Analysis for Industrial Equipment Dalian University of TechnologyDalianChina Chemistry Research Laboratory Department of ChemistryUniversity of OxfordOxfordUK department of Engineering University of CambridgeCambridgeUK

Moisture-responsive actuators are widely used as energy-harvesting devices due to their excellent ability to spontaneously and continuously convert external energy into kinetic ***,it remains a challenge to sustainably synthesize moisture-driven ***,we present a sustainable zero-waste emission methodology to prepare soft actuators using carbon nano-powders and biodegradable polymers through a water evaporation *** to the water solubility and recyclability of the matrixes employed here,the entire synthetic process achieves zero-waste *** composite films featured strong figures of merit and capabilities with a 250◦maximum bending angle under 90%relative *** motions and intelligent bionic applications,including walkers,smart switches,robotic arms,flexible excavators,and hand-shaped actuators,were further achieved by modulating the geometry of the *** sustainable method for actuators’fabrication has great potential in large-scale productions and applications due to its advantages of zero-waste emission manufacturing,excellent recyclability,inherent adaptive integration,and low cost.

关键词： programmable motions recyclability sustainable actuators zero-waste emission

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Increased procoagulant activity of red blood cells in the presence of cisplatin

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Chinese Medical Journal 2008年第18期121卷 1775-1780页

作者： LV Cheng-fang YU Hong-juan HOU Jin-xiao ZHOU Jin department of Hematology First Affiliated Hospital of Harbin Medical University Harbin Heilongjiang 150001 China

Background Cisplatin based chemotherapy is a well recognized risk factor for coagulation disorders and thrombosis. The pathophysiological mechanisms by which cisplatin promote thrombosis are not well understood. Methods Red blood cells （RBCs） were separated from peripheral blood of patients with breast cancer （n=10） and healthy adults （n=6） and treated with cisplatin. Coagulation time of RBCs was assessed by one step recalcification time and the productions of thrombin, intrinsic and extrinsic factor Xa were measured in the presence or absence of various concentrations of lactadherin. Exposed phosphatidylserine was stained with lactadherin and observed by confocal microscopy and flow cytometry. Results Neither fresh RBCs nor RBCs treated without cisplatin had potent procoagulant activity. Cisplatin treatment increased procoagulant activity of RBCs in a cell number- and concentration-dependent manner. Exposed phosphatidylserine was stained with lactadherin and after cisplatin treatment, strong fluorescence was revealed by confocal microscopy. Lactadherin bound RBCs from patients with breast cancer increased from （1.9＋0.5）% on control RBCs to （68.0±3.5）% on RBCs treated with 10umol/L cisplatin for 24 hours. Conclusions Cisplatin treatment increases procoagulant activity of RBCs, which have a strong association with exposure of phosphatidylserine. The increased procoagulant activity may contribute to the pathogenesis of thrombophilia during cisplatin based chemotherapy in breast cancer patients.

关键词： procoagulant activity red blood cell phosphatidylserine cisplatin lactadherin

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Microorganism-derived biological macromolecules for tissue engineering

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Frontiers of Medicine 2022年第3期16卷 358-377页

作者： Naser Amini Peiman Brouki Milan Vahid Hosseinpour Sarmadi Bahareh Derakhshanmehr Ahmad Hivechi Fateme Khodaei Masoud Hamidi Sara Ashraf Ghazaleh Larijani Alireza Rezapour Cellular and Molecular Research Center Iran University of Medical SciencesTehran ***Iran Institutes of Regenerative Medicine Faculty of Advanced Technologies in MedicineIran University of Medical SciencesTehran ***Iran department of Tissue Engineering and Regenerative Medicine Faculty of Advanced Technologies in MedicineIran University of Medical SciencesTehran ***Iran department of Pharmaceutics University of MinnesotaMN 55455USA Burn Research Center Department of Plastic and Reconstructive SurgeryIran University of Medical SciencesTehran ***Iran department of Medical Biotechnology Faculty of ParamedicineGuilan University of Medical SciencesRasht ***Iran department of Biology Science and Research BranchIslamic Azad UniversityTehran ***Iran Cellular and Molecular Research Centre Qom University of Medical SciencesQom ***Iran department of Tissue Engineering and Regenerative Medicine School of MedicineQom University of Medical SciencesQom ***Iran

According to literature,certain microorganism productions mediate biological ***,their beneficial characteristics remain ***,scientists concentrate on obtaining natural materials from live creatures as new sources to produce innovative smart biomaterials for increasing tissue reconstruction in tissue engineering and regenerative *** present review aims to introduce microorganism-derived biological macromolecules,such as pullulan,alginate,dextran,curdlan,and hyaluronic acid,and their available sources for tissue *** evidence indicates that these materials can be used as biological material in scaffolds to enhance regeneration in damaged tissues and contribute to cosmetic and dermatological *** natural-based materials are attractive in pharmaceutical,regenerative medicine,and biomedical *** study provides a detailed overview of natural-based biomaterials,their chemical and physical properties,and new directions for future research and therapeutic applications.

关键词： biological macromolecules regenerative medicine tissue engineering exopolysaccharide carbohydrate

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Comparative studies of versatile extracellular proteolytic activities of lactic acid bacteria and their potential for extracellular amino acid productions as feed supplements

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Journal of animal Science and Biotechnology 2019年第3期10卷 789-801页

作者： Ye Heng Lim Hooi Ling Foo Teck Chwen Loh Rosfarizan Mohamad Norhani Abdullah Institute of Bioscience Universiti Putra Malaysia43400 UPMSerdangSelangorMalaysia department of Bioprocess Technology Faculty of Biotechnology and Biomolecular SciencesUniversiti Putra Malaysia43400 UPMSerdangSelangorMalaysia department of animal Sciences Faculty of AgricultureUniversiti Putra Malaysia43400 UPMSerdangSelangorMalaysia Institute of Tropical Agriculture and Food Security Universiti Putra Malaysia43400 UPMSerdangSelangorMalaysia Institute of Tropical Forestry and Forest Products Universiti Putra Malaysia43400 UPMSerdangSelangorMalaysia

Background:Increasing understanding on the functions of amino acids (AA) has led to new commercial applications and expansion of the worldwide ***,the current technologies rely heavily on non-food grade microorganism and chemical synthesis for the production of *** studies reported that lactic acid bacteria (LAB) have the capability of producing AA owing to their well-established proteolytic system and amino acid biosynthesis ***,the objectives of this study were to explore the extracellular proteolytic activity of LAB isolated from various Malaysian fermented foods and their potential to produce AA extracellularly as feed ***:All the studied LAB isolates were versatile extracellular protease producers,whereby extracellular protease activities were detected from acidic to alkaline pH (pH 5,pH 6.5,pH 8) using qualitative and quantitative proteolytic *** highest proteolytic activity at pH 5 (15.76 U/mg) and pH 8 (19.42 U/mg) was achieved by Lactobacillus plantarum RG14,while Lactobacillus plantarum RS5 exhibited the highest proteolytic activity of 17.22 U/mg at pH *** for the results of AA production conducted in de Man,Rogosa and Sharpe medium and analysed by high pressure liquid chromatography system,all LAB isolates were capable of producing an array of ***,Pediococcus *** greater ability for AA production as compared to Lactobacillus ***,the studied LAB were able to produce a few major feed supplement AA such as methionine,lysine,threonine and *** TL-3 recorded the highest methionine and threonine productivity of 3.72 mg/L/h and 5.58 mg/L/h ***,*** I-UL4 demonstrated a lysine productivity of 1.24 mg/L/h,while *** TP-6 achieved up to 1.73 mg/L/h of tryptophan ***:All the 17 studied LAB isolates possessed versatile extracellular proteolytic system and have vast capability of producing various amino acids including a

关键词： Amino acid Bio-agent Extracel ular proteolytic activity Feed supplement Lactic acid bacteria Lactobacil us Pediococcus

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