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Network pharmacology analysis combined with experimental verification of the molecular mechanism of Xihuang pill in treating liver cancer

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Traditional Medicine Research 2023年第6期8卷 24-32页

作者： Meng-Xin He Ayesha T.Tahir Saba Waris Wen-Bo Cheng Jun Kang School of Life Sciences Tianjin UniversityTianjin 300072China department of Biosciences COMSATS University IslamabadIslamabad 45550Pakistan department of gynacology Allama Iqbal Medical CollegeLahore 54000Pakistan Tianjin Key Laboratory of Medical Mass Spectrometry for Accurate Diagnosis Tianjin 300399China

Background:Xihuang pill is a kind of traditional Chinese medicine,which has been widely used in the treatment of kinds of ***,there is still a lack of systematic understanding of the molecular mechanism of Xihuang pill in the treatment of liver *** this work,we aim to explore the molecular mechanism of Xihuang pill in treating liver ***:The functional components in Xihuang pill were collected from Traditional Chinese Medicine Database and Analysis *** target genes of these components were also collected using Traditional Chinese Medicine Database and Analysis *** target genes of liver cancer were predicted using GeneCards *** intersecting genes were then analyzed with Venn *** Encyclopedia of Genes and Genomes and Database for Annotation,Visualization,and Integrated Discovery were used to analyze the ***,cell counting kit-8 was used to measure the half-maximal inhibitory concentration of Xihuang *** living dead cell staining method was used to observe the survival of ***2 cell apoptosis was tested by flow cytometry with fluorescein isothiocyanate/propidium iodide double staining method,and then the mitochondrial damage was also detected by flow *** expression of target genes was detected by quantitative real-time polymerase chain ***:A total of 130 compounds and 198 genes were identified as potential active ingredients and putative liver cancer‑related *** obtained 1,899 disease targets and 297 transcriptome targets from the *** drug-disease intersecting genes,CCNB1,BIRC5,TOP2A,ESR1,IGF2 and IGFBP3 were *** are enrichment in apoptosis,PI3K-AKT signaling pathway,MAPK signaling pathway,pathways in cancer and p53 signaling ***,it was found that the apoptosis rate of the HepG2 cells in Xihuang pill treated group was significantly higher than that of the control *** the apoptosis rate gradually increased in a dose depe

关键词： Xihuang pill liver cancer network pharmacology p53 signal pathway apoptosis-multiple species pathway

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Prediction and validation of molecular biological mechanism of Fuzheng Huayu capsule in the treatment of liver cancer

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Cancer Advances 2023年第7期6卷 1-9页

作者： Zi-Ning Wang Ayesha T.Tahir Saba Waris Wen-Bo Cheng Jun Kang School of Life Sciences Tianjin UniversityTianjin 300072China department of Biosciences COMSATS University IslamabadIslamabad 45550Pakistan department of gynacology Allama Lqbal Medical CollegeLahore 54000Pakistan Tianjin Key Laboratory of Medical Mass Spectrometry for Accurate Diagnosis Tianjin 300399China

Background:Fuzheng Huayu capsule(FZHY)combined with antiviral treatment has been shown to significantly reduce the risk of liver cancer in patients with hepatitis B ***,the potential of FZHY to directly treat liver cancer remains largely *** study aims to investigate the molecular mechanism underlying the potential of FZHY in treating liver ***:A network pharmacological analysis was performed using the Traditional Chinese Medicine Systems Pharmacology database to identify FZHY compounds and *** targets were searched using the Genecards database,and transcriptome data was downloaded from the NCBI *** Ontology analysis was conducted using the DAVID database,and Kyoto Encyclopedia of Genes and Genomes analysis was based on KOBAS and bioinformatics *** Swissdock database was used for molecular *** cell experiments,the half inhibitory concentration(IC50)of FZHY was determined using the CCK8 *** effects of FZHY on cell viability,apoptosis,and mitochondrial membrane potential were evaluated using a fluorescence microscope and flow *** molecular mechanism of FZHY in treating liver cancer was verified using quantitative polymerase chain ***:A total of 127 compounds and 184 proteins were identified as potential active ingredients and putative liver cancer-related ***,1,899 liver cancer targets,279 transcriptome targets,and 3 pathways(p53 signaling pathway,apoptosis and PI3K-Akt pathway)were *** FZHY-targets-liver cancer interaction network was ***50 of FZHY lyophilized powder solution to liver cancer was 5.13 mg/mL(IC50=5.13 mg/mL).FZHY treatment led to an increase in the ratio of cell apoptosis and induced mitochondrial membrane potential damage,resulting in an increase in the number of dead *** expression levels of CCNB1 and BIRC5 were induced with FZHY treatment,while the expression levels of AKR1C3 and IGF2 were ***

关键词： liver cancer Fuzheng Huayu capsule traditional Chinese medicine CCNB1 BIRC5 AKR1C3 IGF2

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