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Asialoglycoprotein receptor 1 promotes SARS-CoV-2 infection of human normal hepatocytes

Signal Transduction and Targeted Therapy 2024年第3期9卷 1199-1208页

作者： Xinyi Yang Xu Zheng Yuqi Zhu Xiaying Zhao Jun Liu Jiangna Xun Songhua Yuan Jun Chen Hanyu Pan Jinlong Yang Jing Wang Zhimin Liang Xiaoting Shen Yue Liang Qinru Lin Huitong Liang Min Li Fei Peng Daru Lu Jianqing Xu Hongzhou Lu Shibo Jiang Ping Zhao Huanzhang Zhu State Key Laboratory of genetic Engineering and Engineering Research Center of Gene Technology Ministry of EducationInstitute of GeneticsSchool of Life SciencesYiwu Research InstituteFudan UniversityShanghaiChina department of Microbiology Faculty of Naval MedicineNaval Medical UniversityShanghai 200433China Scientific Research Center Shanghai Public Health Clinical CenterFudan UniversityShanghaiChina department of Infectious Diseases and Immunology Shanghai Public Health Clinical CenterFudan UniversityShanghaiChina Changzheng Hospital Naval Medical UniversityShanghaiChina Key Laboratory of Medical Molecular Virology(MOE/NHC/CAMS) Shanghai Institute of Infectious Disease and BiosecuritySchool of Basic Medical SciencesFudan UniversityShanghaiChina department of Infectious Diseases and Nursing Research Institution National Clinical Research Center for Infectious DiseasesThe Third People’s Hospital of ShenzhenShenzhenGuangdongChina

Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)causes multi-organ damage,which includes hepatic dysfunction,as observed in over 50%of COVID-19 *** I converting enzyme(peptidyl-dipeptidase A)2(ACE2)is the primary receptor for SARS-CoV-2 entry into host cells,and studies have shown the presence of intracellular virus particles in human hepatocytes that express ACE2,but at extremely low ***,we asked if hepatocytes might express receptors other than ACE2 capable of promoting the entry of SARS-CoV-2 into *** address this question,we performed a genome-wide CRISPR-Cas9 activation library screening and found that Asialoglycoprotein receptor 1(ASGR1)promoted SARS-CoV-2 pseudovirus infection of HeLa *** Huh-7 cells,simultaneous knockout of ACE2 and ASGR1 prevented SARS-CoV-2 pseudovirus *** the immortalized THLE-2 hepatocyte cell line and primary hepatic parenchymal cells,both of which barely expressed ACE2,SARSCoV-2 pseudovirus could successfully establish an ***,after treatment with ASGR1 antibody or siRNA targeting ASGR1,the infection rate significantly dropped,suggesting that SARS-CoV-2 pseudovirus infects hepatic parenchymal cells mainly through an ASGR1-dependent *** confirmed that ASGR1 could interact with Spike protein,which depends on receptor binding domain(RBD)and N-terminal domain(NTD).Finally,we also used Immunohistochemistry and electron microscopy to verify that SARS-CoV-2 could infect primary hepatic parenchymal *** inhibiting ASGR1 in primary hepatic parenchymal cells by siRNA,the infection efficiency of the live virus decreased ***,these findings indicate that ASGR1 is a candidate receptor for SARS-CoV-2 that promotes infection of hepatic parenchymal cells.

关键词： ACE2 infection glycoprotein

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The spectrum of rare monogenic diseases in patients with premature coronary artery disease

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Chinese Medical Journal 2024年第10期137卷 1246-1248页

作者： Yaqun Teng Tian Du Siqin Feng Ran Tian Yaping Liu Jian Guo Lei Wang Zhiyu Zhang Xiaodong Luan Shan He Shengsheng Zhuang Yifei Wang Shuyuan Zhang Shi Chen Zhenyu Liu Shuyang Zhang department of Cardiology State Key Laboratory of Complex Severe and Rare diseasePeking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China department of Internal Medicine Peking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China department of Basic Medical Sciences Tsinghua University School of MedicineBeijing 100084China department of Breast Oncology Sun Yat-sen University Cancer CenterGuangzhouGuangdong 510060China McKusick-Zhang Center for genetic Medicine State Key Laboratory of Medical Molecular BiologyInstitute of Basic Medical Sciences Chinese Academy of Medical SciencesSchool of Basic Medicine Peking Union Medical CollegeBeijing 100005China Tsinghua University-Peking University Joint Center for Life Sciences Beijing 100084China department of Cardiology Beijing Tsinghua Changgung HospitalTsinghua UniversityBeijing 102218China department of Endocrinology Key Laboratory of Endocrinology of National Health CommissionPeking Union Medical College HospitalChinese Research Center for Behavior Medicine in Growth and DevelopmentBeijing 100730China

To the Editor:The global burden of premature coronary artery disease(CAD)is increasing among the young *** CAD generally refers to the occurrence of obstructive coronary atherothrombotic lesions in men and women aged<55 years and<65 years,respectively,and particularly in those aged<45 years.[1]The incidence of CAD in the younger population has remained stable or has increased,despite the declining incidence in older *** with premature CAD have a poor long-term prognosis,experiencing a high rate of recurrent ischemic events,rapid progression to multivessel disease,and frequent premature death.[1]Thus,it is crucial to identify the underlying causes of premature CAD that are distinct from those of age-related CAD and improve patient outcomes through precise diagnosis and treatment.

关键词： premature younger precise

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Multidrug-Resistant of Escherichia coli and Salmonella spp. Strains in Chicken Feces Intended for Consumption in Open Spaces of Ouagadougou, Burkina Faso

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Open Journal of Applied Sciences 2024年第4期14卷 881-892页

作者： Stéphanie Lynseh Carine Sita Bénao Dabiré Amana Métuor Abdoul Karim Ouattara Rahimatou Yasmine Wendkouni Tiemtoré Nicolas Ouédraogo Blandine Ouédraogo Rhaina Olivia Badini Lionel Eliada Benoit Bambara Serge Sougué Jacques Simporé Biomolecular and genetic Laboratory (LABIOGENE) Pietro Annigoni Biomolecular Research Center (CERBA) Department of Biochemistry-Microbiology University Joseph Ki-Zerbo Ouagadougou Burkina Faso department of Biochemistry-Microbiology University of D&#233dougou D&#233dougou Burkina Faso National Malaria Research and Training Centre (CNRFP) Ouagadougou Burkina Faso

Resistant bacteria can be transmitted to humans through feces or contaminated meat from local chickens. Bacterial strains were isolated from the intestinal contents of 400 local chicken samples from various sales sites. These strains were then characterized using bacteriological and biochemical methods to identify resistant strains. In a study conducted in Ouagadougou, we systematically collected chicken fecal samples from 20 locations across the city, followed by isolation and identification of Salmonella spp. using specific enrichment and culture methods, as well as Escherichia coli. Bacterial strains were characterized using antibiotic resistance profiles were determined through agar diffusion tests, revealing sensitivity or resistance to a range of antibiotics based on established scientific criteria. The results showed that out of the 400 samples collected, 81.25% and 63.5% were contaminated by Escherichia coli and Salmonella spp., respectively. Among these, 86.15% of identified Escherichia coli and 50.78% of Salmonella spp. displayed resistance to at least one tested antibiotic. Among 280 Escherichia coli isolates identified resistant to at least one antibiotic, 31.07% were resistant to cefotaxime (CTX), 20.35% to ceftazidime (CAZ), 21.07% to ceftriaxone (CTR), 75% to amoxicillin clavulanic acid (AMC), 23.57% aztreoname (ATM) and 27.14% were resistant to imipenem (IMP). In the case of the 129 Salmonella spp. isolates resistant to at least one tested antibiotic, 34.88% were resistant to CTX;41.08% to CAZ;35.65% to CTR, 92% to AMC, 39.53% to ATM and finally 47.28% were resistant to IMP. Our study revealed high prevalence of resistance in bacterial strains isolated from local chickens sold outdoors in Ouagadougou. These findings raise significant public health concerns, due to the possible transmission of these resistant strains to humans through the consumption of contaminated meat, thus complicating the treatment of bacterial infections.

关键词： Multidrug-Resistant Chicken Ouagadougou Escherichia coli Salmonella spp. Antibiotic

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OsNPF3.1,a nitrate,abscisic acid and gibberellin transporter gene,is essential for rice tillering and nitrogen utilization efficiency

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Journal of Integrative Agriculture 2024年第4期23卷 1087-1104页

作者： Junnan Hang Bowen Wu Diyang Qiu Guo Yang Zhongming Fang Mingyong Zhang Institute of Rice Industry Technology Research/Key Laboratory of Functional Agriculture Guizhou Provincial Department of Education/Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous RegionMinistry of Education/Key Laboratory of Molecular Breeding for Grain and Oil Crops in Guizhou Province/College of Agricultural SciencesGuizhou UniversityGuiyang 550025China Key Laboratory of South China Agricultural Plant Molecular Analysis and genetic Improvement/Guangdong Provincial Key Laboratory of Applied Botany/South China Botanical Garden Chinese Academy of SciencesGuangzhou 510650China Institute of Fruit Tree Research Guangdong Academy of Agricultural SciencesGuangzhou 510640China

Low-affinity nitrate transporter genes have been identified in subfamilies 4-8 of the rice nitrate transporter 1(NRT1)/peptide transporter family(NPF),but the OsNPF3 subfamily responsible for nitrate and phytohormone transport and rice growth and development remains *** this study,we described OsNPF3.1 as an essential nitrate and phytohormone transporter gene for rice tillering and nitrogen utilization efficiency(NUtE).OsNPF3.1 possesses four major haplotypes of its promoter sequence in 517 cultivars,and its expression is positively associated with tiller *** expression was higher in the basal part,culm,and leaf blade than in other parts of the plant,and was strongly induced by nitrate,abscisic acid(ABA)and gibberellin 3(GA_3)in the root and shoot of *** experiments demonstrated that OsNPF3.1 is a pH-dependent low-affinity nitrate transporter,with rice protoplast uptake assays showing it to be an ABA and GA_3 ***3.1 overexpression significantly promoted ABA accumulation in the roots and GA accumulation in the basal part of the plant which inhibited axillary bud outgrowth and rice tillering,especially at high nitrate *** NUtE of OsNPF3.1-overexpressing plants was enhanced under low and medium nitrate concentrations,whereas the NUtE of OsNPF3.1 clustered regularly interspaced short palindromic repeats(CRISPR)plants was increased under high nitrate *** results indicate that OsNPF3.1 transports nitrate and phytohormones in different rice tissues under different nitrate *** altered OsNPF3.1 expression improves NUtE in the OsNPF3.1-overexpressing and CRISPR lines at low and high nitrate concentrations,respectively.

关键词： rice tillering grain yield phytohormone nitrate transporter nitrogen utilization efficiency

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Stimulatory Effect of Tithonya diversifolia-by Products on Plantain Banana Vivoplants in Nursery (A Review)

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American Journal of Plant Sciences 2024年第9期15卷 726-745页

作者： Cécile Annie Ewané Arouna Meshuneke Gaston Elock Mbang Fabrice Damien Wassom William Asah Che Glen Takang Beyang Craftsman Ndula Ndula-Nan Landry Fotsing Silatsa Jospin Walter Kom Timma Marie-Paule Kengoum Djam Rosine Arckange Barakielle Bindzi Abah Nicolas Niemenak department of Biochemistry Faculty of Science University of Yaounde 1 Yaounde Cameroon Laboratory of Biochemistry and Vegetal Physiology of Higher Teachers Training College University of Yaounde 1 Yaounde Cameroon Laboratory of Phytoprotection and Valorisation of genetic Resources of The Biotechnology Centre University of Yaounde 1 Yaounde Cameroon department of Biological Science Higher Teachers Training College University of Yaounde 1 Yaounde Cameroon

Plantain banana is an important cash crop that serves as stable food for millions of people around the world and contributes to income generation. Indeed, they provide a major staple food crop for millions of people and play an important role in the social fabric of many rural communities. Plantain banana cultivation encounters major problem of seedlings unavailability that are essential for the creation of new plantations, as well as parasitic constraints. Mycosphaerella fijiensis is the main pathogen attack constraints of banana plant responsible of black Sigatoka disease, and viruses, which can severely reduce the photosynthetic leaf area, leading to banana production losses of more than 80% in plantations with soil fertility problems. The repeated use of synthetic input is the origin of contamination to the environment, different pollution sources of plants and human health, as well as resistance to some strains of pathogens and plant fertilization problems over time. Recent works carried out in nursery have shown that vivoplants of plantains treated with biostimulants based on natural products notably Tithonia diversifolia biopromote good growth and less susceptibility to M. fijiensis. Indeed, an increase in agromorphological characteristics, good accumulation of growth and defense biomarkers was also observed. In this context, Tithonia diversifolia is shown to be involved in the stimulatory effect mechanism of growth promotion and defensive reaction of plantain vivoplants against various pathogens and it is suggested to be acting as a vital stimulator. This article reviews the current state of knowledge on plantain banana cultivation constraints and on the potential of Tithonia diversifolia in relation with its different stimulatory effects on plantain vivoplants.

关键词： Tithonia diversifolia Plantain Banana Black Sigatoka Disease Growth Biopromotion Bioprotection Induced Resistance Biofungicidal Effect

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Spectrum of NSD1 gene mutations in southern Chinese patients with Sotos syndrome

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Chinese Medical Journal 2005年第18期118卷 1499-1506页

作者： Tony M.F. Tong Edgar W.L. Hau Ivan F.M. Lo Daniel H.C. Chan Stephen T.S. Lam Clinical genetic Service Department of Health Hong Kong SAR China Clinical Genetic Service Department of Health Hong Kong SAR China Clinical Genetic Service Department of Health Hong Kong SAR China Clinical Genetic Service Department of Health Hong Kong SAR China Clinical Genetic Service Department of Health Hong Kong SAR China

Background Sotos syndrome is an overgrowth syndrome with characteristic facial gestalt and mental retardation of variable severity. Haploinsufficiency of the NSD 1 gene has been implicated as the major cause of Sotos syndrome, with a predominance of microdeletions reported in Japanese patients. This study was conducted to investigate into the spectrum of NSD1 gene mutations in southern Chinese patients with Sotos syndrome. Methods Thirty-six Chinese patients with Sotos syndrome and two patients with Weaver syndrome were subject to molecular testing. Results NSD1 gene mutations were detected in 26 （72%） Sotos patients. Microdeletion was found in only 3 patients, while the other 23 had point mutations （6 frameshift, 8 nonsense, 2 spice site, and 7 missense）. Of these, 19 mutations were never reported. NSD1 gene mutations were not found in the two patients with Weaver syndrome. Conclusions Most cases of Sotos syndrome are caused by NSD1 gene defects, but the spectrum of mutations is different from that of Japanese patients. Genotype-phenotype correlation showed that patients with microdeletions might be more prone to congenital heart disease but less likely to have somatic overgrowth. The two patients with Weaver syndrome were not found to have NSD1 gene mutations, but the number was too small for any conclusion to be drawn.

关键词： Sotos syndrome·Weaver syndrome·NSD1 gene mutation

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Phage-displayed heptapeptide sequence conjugation significantly improves the specific targeting ability of antimicrobial peptides against Staphylococcus aureus

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mLife 2024年第2期3卷 251-268页

作者： Tao Wang Peng Tan Qi Tang Chenlong Zhou Yakun Ding Shenrui Xu Mengda Song Huiyang Fu Yucheng Zhang Xiaohui Zhang Yueyu Bai Zhihong Sun Xi Ma State Key Laboratory of Animal Nutrition and Feeding College of Animal Science and TechnologyChina Agricultural UniversityBeijingChina Luoyang Key Laboratory of Animal genetic and Breeding College of Animal ScienceHenan University of Science and TechnologyLuoyangChina Key Laboratory of Innovative Utilization of Indigenous Cattle and Sheep Germplasm Resources(Co‐construction by Ministry and Province) Ministry of Agriculture and Rural AffairsZhengzhou UniversityZhengzhouChina Animal Health Supervision in Henan Province ZhengzhouChina Laboratory for Bio‐Feed and Molecular Nutrition Department of Animal Science and TechnologySouthwest UniversityChongqingChina

Broad-spectrum antibacterial drugs often lack specificity,leading to indiscriminate bactericidal activity,which can disrupt the normal microbial balance of the host flora and cause unnecessary cytotoxicity during systemic *** this study,we constructed a specifically targeted antimicrobial peptide against Staphylococcus aureus by introducing a phage-displayed peptide onto a broad-spectrum antimicrobial peptide and explored its structure–function relationship through one-factor ***2 obtained by screening based on the selectivity index and the targeting index showed specific killing ability against ***,SFK2 showed excellent biocompatibility in mice and piglet,and demonstrated significant therapeutic efficacy against *** *** conclusion,our screening of phage-derived heptapeptides effectively enhances the specific bactericidal ability of the antimicrobial peptides against ***,providing a theoretical basis for developing targeted antimicrobial peptides.

关键词： antimicrobial peptides membrane disruption mechanism microbial infection phage display technology Staphylococcus aureus

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Robust neutralization of SARS-CoV-2 variants including JN.1 and BA.2.87.1 by trivalent XBB vaccine-induced antibodies

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Signal Transduction and Targeted Therapy 2024年第6期9卷 2359-2361页

作者： Xun Wang Shujun Jiang Wentai Ma Yanliang Zhang Pengfei Wang Shanghai Pudong Hospital Fudan University Pudong Medical CenterState Key Laboratory of Genetic EngineeringMOE Engineering Research Center of Gene TechnologySchool of Life SciencesShanghai Institute of Infectious Disease and BiosecurityFudan UniversityShanghaiChina department of Infectious Diseases Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese MedicineNanjing Research Center for Infectious Diseases of Integrated Traditional Chinese and Western MedicineNanjingJiangsuChina Beijing Institute of Genomics Chinese Academy of SciencesUniversity of Chinese Academy of Sciences and China National Center for BioinformationBeijingChina

Dear Editor,The rapid and widespread transmission of SARS-CoV-2 has led to the emergence of multiple variants of concern(VOCs),most notably the Omicron variant,which continues to evolve and diversify into a range of *** previous research has shown that these Omicron sub-lineages,spanning BA.1-BA.2.86,have been evolving to exhibit increased neutralization escape capabilities.

关键词： evolve neutralization variants

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Response to Commentary by Dr. Matthew J. Clarkson on

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Journal of Sport and Health Science 2024年第5期13卷 670-671页

作者： Hugo de Luca Corrêa Lysleine Alves Deus Dahan da Cunha Nascimento Nicholas Rolnick Rodrigo Vanerson Passos Neves Andrea Lucena Reis Thais Branquinho de Araújo Carmen Tzanno-Martins Fernanda Silveira Tavares Luiz Sinésio Silva Neto Cláudio Avelino Rodrigues Santos Paolo Lucas Rodrigues-Silva Fernando Honorato Souza Vitória Marra da Motta Vilalva Mestrinho Rafael Lavarini dos Santos Rosangela Vieira Andrade Jonato Prestes Thiago dos Santos Rosa Post-graduate Program of Physical Education Catholic University of BrasıliaBrasılia 71966-700Brazil The Human Performance Mechanic Lehman CollegeNew YorkNY 10011USA Clinical Group Home Dialysis Center and RenalClass Sao Paulo 01239-040Brazil department of Medicine Catholic University of BrasıliaBrasılia 71966-700Brazil Faculty of Medicine Federal University of TocantinsTocantins 77001-090Brazil Embrapa genetic Resources and Biotechnology Brasılia 70770-917Brazil g Graduate Program of Genomic Sciences and Biotechnology Brasılia 71966-700Brazil

Dear editor:We sincerely thank Matthew *** for his insightful commentary1 on our recent publication in the Journal of Sport and Health Science.2 Clarkson's pioneering work in the development and application of blood flow restriction (BFR) exercise protocols has undeniably laid the groundwork for subsequent investigations in this field,including our own.(3-6)His commentary not only highlights the relevance and timeliness of our study but also offers valuable perspectives on refining safety considerations for intradialytic BFR exercise among hemodialysis patients.

关键词： patients application flow

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TaRLK-6A promotes Fusarium crown rot resistance in wheat

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Journal of Integrative Plant Biology 2024年第1期66卷 12-16页

作者： Haijun Qi Xiuliang Zhu Wenbiao Shen Xia Yang Chaozhong Zhang Genying Li Feng Chen Xuening Wei Zengyan Zhang Institute of Special Animal and Plant Sciences Chinese Academy of Agricultural SciencesChangchun 130000China The National Key Facility for Crop Gene Resources and genetic Improvement Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China College of Life Science Nanjing Agricultural UniversityNanjing 210095China Agronomy College/National Key Laboratory of Wheat and Maize Crop Science/CIMMYT‐China(Henan)Joint Center of Wheat and Maize Henan Agricultural UniversityZhengzhou 450002China department of Plant Sciences University of CaliforniaDavisCA 95616USA Crop Research Institute Shandong Academy of Agricultural SciencesJinan 250100China

Fusariumcrown rot(FCR),mainly caused by the soil‐borne fungus Fusarium pseudograminearum,is a devastatingdisease of wheat(Triticum aestivum(Ta)).Fusariumcrown rotcauses substantial yield losses and generates mycotoxins inwheat grains that can cause serious health problems in hu-mans and livestock(Powell et al.,2017).Identifying genes thatprovide resistance *** character-izing the molecular mechanisms underlying such resistance arekey to breeding wheat varieties that are resistant to this dev-astating fungus.

关键词： aestivum breeding soil

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