检索结果-南通市图书馆

The non-coding RNA SVALKA locus produces a cis-natural antisense transcript that negatively regulates the expression of CBF1 and biomass production at normal temperatures

在线全文

学校读者我要写书评

暂无评论

plant Communications 2023年第4期4卷 77-87页

作者： Vasiliki Zacharaki Shiv Kumar Meena Peter Kindgren Umeåplant Science Centre Department of Forest Genetics and Plant PhysiologySwedish University of Agricultural Sciences90187 Umea°Sweden

Non-coding transcription is present in all eukaryotic genomes,but we lack fundamental knowledge about its importance for an organism’s ability to develop *** plants,emerging evidence highlights the essential biological role of non-coding transcription in the regulation of coding ***,we have few molecular insights into this ***,we show that a long isoform of the long noncoding RNA SVALKA-L(SVK-L)forms a natural antisense transcript to the host gene CBF1 and negatively regulates CBF1 mRNA levels at normal temperatures in the model plant Arabidopsis ***,we show detailed evidence for the specific mode of action of *** pathway includes the formation of double-stranded RNA that is recognized by the DICER proteins and subsequent downregulation of CBF1 mRNA ***,the CBF1-SVK regulatory circuit is not only important for its previously known role in cold temperature acclimation but also for biomass production at normal *** study characterizes the developmental role of SVK-L and offers mechanistic insight into how biologically important overlapping natural antisense transcripts can act on and fine-tune the steady-state levels of their host gene’s mRNA.

关键词： SVALKA cis-natural antisense transcript non-coding transcription CBF1 Arabidopsis

Getting started in mapping-by-sequencing

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative plant Biology 2015年第7期57卷 606-612页

作者： Héctor Candela Rubén Casanova-Sáez José Luis Micol Instituto de Bioingeniería Universidad Miguel Hernández department of forest genetics and plant physiology Umea Plant Science CenterSwedish University of Agricultural Sciences

Next-generation sequencing （NGS） technologies allow the cost-effective sequencing of whole genomes and have expanded the scope of genomics to novel applications, such as the genome-wide characterization of intraspecific polymorphisms and the rapid mapping and identification of point mutations. Next-generation sequencing platforms, such as the Illumina HiSeq2ooo platform, are now commercially available at affordable prices and routinely produce an enormous amount of sequence data, but their wide use is often hindered by a lack of knowledge on how to manipulateand process the information produced. In this review, we focus on the strategies that are available to geneticists who wish to incorporate these novel approaches into their research but who are not familiar with the necessary bioinformatic concepts and computational tools. In particular, we comprehensively summarize case studies where the use of NGS technologies has led to the identification of point mutations, a strategy that has been dubbed ＂mapping-by-sequencing＇, and review examples from plants and other model species such as Caenorhabditis elegans, Saccharomyces cerevisiae, and Drosophila mela- nogaster. As these technologies are becoming cheaper and more powerful, their use is also expanding to allow mutation identification in species with larger genomes, such as many crop plants.

关键词： Mapping-by-sequencing massively parallel sequencing mutation identification SHOREmapping whole-genome re-sequencing

Poplar wood-inside out:High-resolution spatial,cellular,and pseudotime projections from cambial transcriptomes

在线全文

学校读者我要写书评

暂无评论

Molecular plant 2023年第10期16卷 1490-1492页

作者： Shruti Choudhary Hannele Tuominen Umeåplant Science Centre Department of Forest Genetics and Plant PhysiologySwedish University of Agricultural Sciences90183 UmeåSweden

The lifestyle of trees may seem sedentary,but behind the scenes,various meristems are busy stacking layers of cells in differentdirections,which allows trees to soar to great heights and *** cells are formed continuously during"primarygrowth"very close to the shoot apex,guaranteeing the continuityof the vascular system throughout the stem and vertical *** growth turns,by definition,into"secondary growth"with the advent of the vascular cambium.

关键词： guarantee directions continuously

The Role of Viral Infection in Inducing Variability in Virus-Free Progeny in Tomato

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative plant Biology 2009年第5期51卷 476-488页

作者： Liliana Marii Gheorghe Chiriac Institute of genetics and plant physiology Academy of Sciences of Moldova

The effect of virus-host interactions on subsequent generations is poorly understood. The evaluation of the effects of viral infection on inheritance of quantitative traits in the progeny of infected plants and elucidation of a possible relationship between chiasma frequency in the infected plants and variability of traits in the progeny were investigated. The current study involved genotypes of four intraspecific hybrids of tomato （Solanum lycopersicum L.）, their parental forms and two additional cultivars. Used as infection were the tobacco mosaic virus （TMV） and potato virus X （PVX）. The consequences of the effect of viral infection were evaluated based on chromosome pairing in diakinesis and/or by examining quantitative and qualitative traits in the progeny of the infected tomato plants. Tomato plants infected with TMV ＋ PVX were found to differ in chiasma frequency per pollen mother cell or per bivalent. Deviations have been observed for genotypes of both F1 hybrids and cultivars. At the same time, differences in mean values of the traits under study have only been found for progeny populations （F2-F4） derived from virus-infected F1 hybrids, but not in the case of progeny of the infected cultivars. The rate of recombinants combining traits of both parents increased significantly （2.22-8.24 times） in progeny populations of hybrids infected with TMV＋PVX. The above suggests that the observed effects could be the result of modification of recombination frequencies that can be manifested in heterozygous hybrids and make small contributions to variability in cases of ＇homozygous＇ tomato genotypes （i.e. cultivars）.

关键词： chiasma recombination Solanum lycopersicum traits variability viral infection.

Agrobacterium tumefaciens-mediated transformation of rice with the spider insecticidal gene conferring resistance to leaffolder and striped stem borer

在线全文

学校读者我要写书评

暂无评论

Cell Research 2001年第2期11卷 149-155页

作者： HUANG JIAN QIU, ZHI MING WEI, HAI LONG AN, YU XIAN ZHU National Laboratory of plant Molecular genetics, Institute of plant physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China National La National Laboratory of plant Molecular genetics Institute of Plant Physiology and Ecology Chinese Academy of Sciences Shanghai. jqhuang@***

Immature embryos of rice varieties 'Xiushui11' and 'Chunjiang 11' precultured for 4d were infected and transformed by Agrobacterium tumefaciens strain EHA101/pExT7 (containing the spider insecticidal gene). The resistant cant were transferred onto the differentiation medium and plants were regenerated. The transformation frequency reached 56%-72% measured as numbers of Geneticin (G418)-resistant calli produced and 36%-60% measured as numbers of transgenic plants regenerated, respectively. PCR and Southern blot analysis of transgenic plants confirmed that the T-DNA had been integrated into the rice genome. Insect bioassays using T1 transgenic plants indicated that the mortality of the leaffolder (Cnaphalocrasis medinalis) after 7d of leaf feeding reached 38%-61% and the corrected mortality of the striped stem borer (Chilo suppressalis) after 7d of leaf feeding reached 16%-75%. The insect bioassay results demonstrated that the transgenic plants expressing the spider insecticidal protein conferred enhanced resistance to these pests.

关键词： Rice Agrobacterium tumefaciens spider insecticidal gene transgenic plant Leaffolder striped stem borer insect bioassay.

CRISPR Primer Designer:Design primers for knockout and chromosome imaging CRISPR-Cas system

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative plant Biology 2015年第7期57卷 613-617页

作者： Meng Yan Shi-Rong Zhou Hong-Wei Xue National Key Laboratory of plant Molecular genetics Shanghai Institute of Plant Physiology and Ecologythe Chinese Academy of Sciences

The clustered regularly interspaced short palin- dromic repeats （CRiSPR）-associated system enables biologists to edit genomes precisely and provides a powerful tool for perturbing endogenous gene regulation, modulation of epigenetic markers, and genome architecture. However, there are concerns about the specificity of the system, especially the usages of knocking out a gene. Previous designing tools either were mostly built-in websites or ran as command-line programs, and none of them ran locally and acquired a user- friendly interface. In addition, with the development of CRISPR-derived systems, such as chromosome imaging, there were still no tools helping users to generate specific end-user spacers. We herein present researchers to design primers CRISPR Primer Designer for for CRISPR applications. Theprogram has a user-frfendly interface, can analyze the BLAST results by using multiple parameters, score for each candidate spacer, and generate the primers when using a certain plasmid. In addition, CRISPR Primer Designer runs locally and can be used to search spacer clusters, and exports primers for the CRiSPR-Cas system-based chromosome imaging system.

关键词： Chromosome imaging CRISPR knockout primer software

The ethical cost of doing nothing

在线全文

学校读者我要写书评

暂无评论

National Science Review 2020年第7期7卷 1260-1262页

作者： Andrew J.Parker University of Oxford Department of PhysiologyAnatomy and Genetics

Concern has been raised about the ethics and risks of performing genetic interventions in macaque monkeys to study models of human neuropsychiatric conditions. Here I point out that,when the outcome of some situations is truly uncertain, human decision makers tend to evaluate these situations inconsistently. The consequences of this inconsistency are sometimes profound, as they lead policy makers and reviewers to ignore or discount the ethical costs of doing nothing. I conclude that there are fundamental problems with the ‘precautionary

关键词：

Molecular characterization of chalcone isomerase (CHI) regulating flower color in herbaceous peony (Paeonia lactiflora Pall.)

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2018年第1期17卷 122-129页

作者： WU Yan-qing ZHU Meng-yuan JIANG Yu ZHAO Da-qiu TAO Jun Jiangsu Key Laboratory of Crop genetics and physiology College of Horticulture and Plant ProtectionYangzhou University

Chalcone isomerase （CHI） is a key enzyme that converts yellow chalcone to colorless naringenin, playing an important regulatory role in color formation of ornamental flowers. We determined the coding sequence of CHI in herbaceous peony using rapid-amplification of cDNAends （RACE） technology, and subsequently detected the expression pattern of CHI in the inner and outer petals at different developmental stages using qRT-PCR. We cloned the upstream promoter sequences of CHI using genome walking technology and predicted the location of CpG islands and 5＇ truncation. In addition, we con- structed five dual-luciferase reporter gene carriers and detected the promoter activities of different fragments. Our results showed that the full-length cDNA sequence of CHI was 898 bp, and the 5＂-upstream core promoter was located at -1 651 to -2050 bp region, where contained one CpG island （-1 897 to -2010 bp） and several important binding sites of transcription factor, such as Spl, serum response factor （SRF）, activating protein （AP）-2alpha and CCAAT/enhancer binding protein （C/ EBP）alpha. Expression results showed that the expression of CHI at different developmental stages was generally higher in inner petals than those in outer petals, and the maximum at the bud stage （S1）. Thus, this study will provide theoretical basis for an in-depth study of CHI gene function and expression regulation.

关键词： herbaceous peony CHI gene cloning promoter transcriptional activity

SRG1,Encoding a Kinesin-4 Protein,Is an Important Factor for Determining Grain Shape in Rice

在线全文

学校读者我要写书评

暂无评论

Rice science 2018年第6期25卷 297-307页

作者： QIN Ran Delara AKHTER YANG Chengcong Ujjal Kumar NATH Jamal ESHAG JIN Xiaoli SHI Chunhai department of Agronomy Zhejiang University department of genetics and plant Breeding Sylhet Agricultural University department of genetics and plant Breeding Bangladesh Agricultural University

Grain shape is one of the important agronomic traits, which is closely related to the yield of rice.A new rice mutant, named small and round grain(srg1), was isolated from an indica cultivar Zhenong 34 by ethyl methane sulfonate(EMS) mutagenesis. The microscopic analysis showed that the cell length of spikelet in srg1 was decreased compared with that in wild type(WT), which caused the grain length ***, the grains of srg1 were wider than those of WT because of the increased cell layers in spikelet in the lateral direction. Therefore, the inhibition of cell expansion and increased cell proliferation collectively led to the small and round grain. By map-based cloning, the gene SRG1 was located on the short arm of chromosome 9, which encodes a kinesin-4 protein, represented by the gene LOC_Os09 g02650. A single nucleotide polymorphism, occurred in the 16 th exon of SRG1, led to premature translation stop in mutant. The cell cycle-related genes were up-regulated in srg1, which conferred that SRG1 controlled grain width through the cell proliferation. Since the role of SRG1 in regulating grain shape was not clarified well before, it is valuable to explore the mechanism of grain development. This study could, hence, provide a morphogenesis and molecular basis for elucidating the function of SRG1, as well as a new germplasm resource for the further study of grain development.

关键词： small and round grain kinesin-4 protein gene mapping rice