检索结果-南通市图书馆

Dissecting the cell microenvironment of ovarian endometrioma through single-cell RNA sequencing

science China Life sciences 2025年第1期 116-129页

作者： Jiangpeng Wu Siyu Xia Wenting Ye Yan Sun Jing Cai Fubing Yu Haiping Wen Xiuwei Yi Taikang Li Mingwei Chen Jiayun Chen Ge Song Chuanbin Yang Yali Song Jigang Wang department of Reproductive medicine Dongguan Maternal and Child Health Care Hospital department of Urology and Shenzhen Clinical Research Centre for GeriatricsShenzhen People's HospitalSouthern University of Science and Technology State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs Artemisinin Research Centerand Institute of Chinese Materia MedicaChina Academy of Chinese Medical Sciences Reproductive medicine Center Foshan Women and Children Hospital State Key Laboratory of Antiviral Drugs School of PharmacyHenan University

Ovarian endometrioma（OE）, also known as “chocolate cysts,” is a cystic mass that develops in the ovaries due to endometriosis and is a common gynecological condition characterized by the growth of endometrial tissue outside the uterus, leading to symptoms such as dysmenorrhea, pelvic pain, and infertility. However, the precise molecular and cellular mechanisms driving this pathophysiology remain largely unknown, posing challenges for diagnosis and treatment. Here, we employed integrated single-cell transcriptomic profiling of over 52,000 individual cells from endometrial tissues of OE patients and healthy donors and identified twelve major cell populations. We identified notable alterations in cell type-specific proportions and molecular signatures associated with OE. Notably, the activation of IGFBP5+macrophages with pro-inflammatory properties, NK cell exhaustion, and aberrant proliferation of IQCG+and KLF2+epithelium are key features and may be the potential mechanisms underlying the pathogenesis of OE. Collectively, our data contribute to a better understanding of OE at the single cell level and may pave the way for the development of novel therapeutic strategies.

关键词： ovary high-throughput sequencing transcriptomics gene expression analysis ligand-receptor analysis

来源：

同方期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Current state and progress of research on forensic biomechanics in China

引用

forensic sciences Research 2021年第1期6卷 1-12页

作者： Yijiu Chen shanghai Key Lab of forensic medicine Key Lab of Forensic scienceMinistry of Justiceshanghai Forensic service Platformacademy of Forensic scienceshanghaichina

forensic biomechanics gradually has become a significant component of forensic *** biomechanics is evidence-based science that applies biomechanical principles and methods to forensic practice,which has constituted one of the most potential research *** this review,we introduce how finite element techniques can be used to simulate forensic cases,how injury criteria and injury scales can be used to describe injury severity,and how tests of postmortem human subjects and dummy can be used to provide essential validation *** review also describes research progress and new applications of forensic biomechanics in China.

关键词： forensic sciences forensic biomechanics finite element analysis multiple rigid body postmortem human subjects(PHMS) injury reconstruction injury criteria material property driver identification anthropometric test devices injury biomechanism

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Preliminary study on the mechanisms of ankle injuries under falling and impact conditions based on the THUMS model

引用

forensic sciences Research 2022年第3期7卷 518-527页

作者： Zhengdong Li Jianhua Zhang Jinming Wang Ping Huang Donghua Zou Yijiu Chen department of forensic medicine School of Basic Medical SciencesFudan UniversityShanghaiChina Shanghai Key laboratory of forensic medicine Academy of Forensic ScienceMinistry of JusticeShanghaiChina

Ankle injuries are common in forensic practice,which are mainly caused by falling and traffic *** the mechanisms and manners of ankle injuries is a critical and challenging problem for forensic *** identification of the injury mechanism is still experience-based and strongly *** also lacks systematic research in current *** our study,based on the widely used Total Human Model of Safety 4.0(THUMS 4.0),we utilized the finite element(FE)method to simulate ankle injuries caused by falls from different heights(5 m,10 m and 20 m)with different landing postures(natural posture,inversion,eversion,plantar-flexion and dorsi-flexion)and injuries caused by impacts from different directions(anterior-posterior,lateral-medial and posterior-anterior)with different speeds(10 m/s,15m/s and 20m/s)at different sites(ankle and lower,middle and upper sections of leg).We compared the injury morphology and analyzed the mechanisms of ankle *** results showed that falling causes a specific compression fracture of the distal tibia,while fractures of the tibia and fibula diaphysis and ligament injuries caused by falling from a lower height or inversion,planter flexion or dorsiflexion at a large angle are not distinguishable from the similar injury patterns caused by impact on the middle and upper segments of the *** obvious compression fracture of the tibia distal was caused by the impacts,whereas ligament injuries and avulsion fractures of the medial or lateral condyle and fractures of the diaphysis of the tibia and fibula were *** studies will be helpful in reconstructing the ankle injury processes and analyzing the mechanisms in forensic practice,providing a deeper understanding of ankle injury mechanisms for forensic experts.

关键词： forensic sciences ankle injuries fall and impact injury finite element analysis injury biomechanisms

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Wave effect of gravitational waves intersected with a microlens field Ⅱ:An adaptive hierarchical tree algorithm and population study

引用

science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 241-254页

作者： Xikai Shan Guoliang Li Xuechun Chen Wen Zhao Bin Hu Shude Mao department of Astronomy Tsinghua University Purple Mountain Observatory Chinese Academy of Sciences Institute for Frontier in Astronomy and Astrophysics Beijing Normal University School of Physics and Astronomy Beijing Normal University School of Astronomy and Space science University of Science and Technology of China CAS Key Laboratory for Researches in Galaxies and Cosmology Department of Astronomy University of Science and Technology of ChinaChinese Academy of Sciences

The gravitational lensing wave effect generated by a microlensing field embedded in a lens galaxy is an inevitable phenomenon in strong lensed gravitational waves （SLGWs）.This effect presents both challenges and opportunities for the detection and application of ***,investigating this wave effect requires computing a complete diffraction integral over each microlens in the *** is extremely time-consuming due to the large number of microlenses （10～3-10～6）.Therefore,simply adding all the microlenses is ***,the complexity of the time delay surface makes the lens plane resolution a crucial factor in controlling numerical *** this paper,we propose a trapezoid approximation-based adaptive hierarchical tree algorithm to meet the challenges of calculation speed and *** find that this algorithm accelerates the calculation by four orders of magnitude compared to the simple adding method and is one order of magnitude faster than the fixed hierarchical tree algorithm proposed for electromagnetic *** importantly,our algorithm ensures controllable numerical errors,increasing confidence in the *** with our previous work （***.66,239511,2023）,this paper addresses all numerical issues,including integral convergence,precision,and computational time1).Finally,we conducted a population study on the microlensing wave effect of SLGWs using this algorithm and found that the microlensing wave effect cannot be ignored,especially for Type II SLGWs （from saddle position of the time delay surface） due to their intrinsic geometric structures and their typical intersection with a denser microlensing ***,more than 33%（11%） of SLGWs have a mismatch larger than 1%（3%） compared to the unlensed ***,we found that the mismatch between signal pairs in a doubly imaged GW is generally larger than 10-3,and 61%（25%） of signal pairs have a mismatch larger than 1%（3%）.Therefo

关键词： gravitational lensing diffractional integral algorithm gravitational wave

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Direct and rapid detection of serum amino acid and monoamine neurotransmitters to assist the diagnosis of panic disorder

引用

science China Chemistry 2025年第01期 377-384页

作者： Shiwen Liu Xuerui Wan Meng Zhao Jiaqi Wang Weilan Wu Linlin You Yonggui Yuan Qian Xu Rong Gao Key Laboratory of Environmental medicine Engineering Ministry of Education School of Public Health Southeast University Key Laboratory of Modern Toxicology of Ministry of Education Department of Hygienic Analysis and Detection School of Public HealthNanjing Medical University department of Psychosomatics and Psychiatry Zhongda Hospital School of Medicine Southeast University

The measurement of amino acid and monoamine neurotransmitters（NTs） in serum plays a central role in the identification and monitoring of clinical panic disorder（PD）. However, the strong polarity and chemical heterogeneity of NTs make direct extraction and rapid detection extremely challenging. Herein, we developed a strategy that integrated solid phase extraction based on magnetic Fe3O4coated with polydopamine（Fe3O4@PDA） and direct analysis of mass spectrometry in real time（DART-MS） for quantification of 10 NTs. We systematically investigated the optimal conditions of this strategy to achieve good accuracy and precision. Compared with existing magnetic solid phase extraction methods for NTs detection in biological samples, this strategy could adsorb more targets while having similar adsorption efficiencies, and the total process time was reduced by an average of 69.42%. We rapidly determined the metabolic abnormality of 6 types of NTs in the serum of PD patients and demonstrated their diagnostic accuracy. In addition, an outstanding feature of PD in which tryptophan was more metabolized by the KYN pathway and the 5-hydroxytryptamine pathway was inhibited could be elucidated by this strategy. Our results highlighted the potential ability of Fe3O4@PDA coupled with DART-MS to assist in the clinical diagnosis of PD.

关键词：

来源：

同方期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Protein arginine methyltransferase-6 regulates heterogeneous nuclear ribonucleoprotein-F expression and is a potential target for the treatment of neuropathic pain

引用

Neural Regeneration Research 2025年第9期20卷 2682-2696页

作者： Xiaoyu Zhang Yuqi Liu Fangxia Xu Chengcheng Zhou Kaimei Lu Bin Fang Lijuan Wang Lina Huang Zifeng Xu department of Anesthesiology International Peace Maternity and Child Health HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghaiChina department of Anesthesiology Shanghai General HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghaiChina Shanghai Key Laboratory of Embryo Original Diseases ShanghaiChina

Protein arginine methyltransferase-6 participates in a range of biological functions,particularly RNA processing,transcription,chromatin remodeling,and endosomal ***,it remains unclear whether protein arginine methyl transferase-6 modifies neuropathic pain and,if so,what the mechanisms of this *** this study,protein arginine methyltransferase-6 expression levels and its effect on neuropathic pain were investigated in the spared nerve injury model,chronic constriction injury model and bone cancer pain model,using immunohistochemistry,western blotting,immunoprecipitation,and label-free proteomic *** results showed that protein arginine methyltransferase-6 mostly co-localized withβ-tubulinⅢin the dorsal root ganglion,and that its expression decreased following spared nerve injury,chronic constriction injury and bone cancer *** addition,PRMT6 knockout(Prmt6~(-/-))mice exhibited pain ***,the development of spared nerve injury-induced hypersensitivity to mechanical pain was attenuated by blocking the decrease in protein arginine methyltransferase-6 ***,when protein arginine methyltransferase-6 expression was downregulated in the dorsal root ganglion in mice without spared nerve injury,increased levels of phosphorylated extracellular signal-regulated kinases were observed in the ipsilateral dorsal horn,and the response to mechanical stimuli was ***,protein arginine methyltransferase-6 appeared to contribute to spared nerve injury-induced neuropathic pain by regulating the expression of heterogeneous nuclear ***,protein arginine methyltransfe rase-6-mediated modulation of hete rogeneous nuclear ribonucleoprotein-F expression required amino atids 319 to 388,but not classical H3R2 *** findings indicated that protein arginine methyltransferase-6 is a potential therapeutic target fo r the treatment of peripheral neuro pathic pain.

关键词： dorsal root ganglion heterogeneous nuclear ribonucleoprotein F neuropathic pain protein arginine methyltransferase-6 sensory neurons

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Fatal meningococcal meningitis in a 2-year-old child:A case report

引用

World Journal of Clinical Cases 2019年第5期7卷 636-641页

作者： Aleksander Mularski Czesław Żaba department of Legal and forensic medicine Poznan University of Medical Sciences Institute of forensic Research

BACKGROUND In fatal cases of meningococcal septicemia, bacteriological diagnosis may not be straightforward due to postmortem replication and relocation of endogenic microflora. In medicolegal practice, aside from routine autopsy and histopathology, also other diagnostic methods, such as microbiological tests,immunohistochemistry and polymerase chain reaction(PCR), are used to examine body fluids and *** SUMMARY We present the case of sudden death in a 2-year-old child. The patient died approximately 30 min after hospital admission before any routine diagnostic procedures were undertaken. Presence of whole-body rash and fulminant course of the disease raised suspicion of meningococcal septicemia. An autopsy was performed seven days after death when the body showed the signs of late postmortem decomposition. No etiological factor of septicemia could be identified based on macro-and microscopic findings. However, PCR demonstrated the presence of genetic material of group W Neisseria meningitidis in patient's cerebrospinal fluid and *** Microbiological PCR should be conducted postmortem whenever a specific etiological factor could not be identified with conventional methods.

关键词： Meningococcal infection Molecular microbiology Waterhouse-Friderichsen syndrome Neisseria meningitidis Autopsy Cerebrospinal fluid Case report

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Use of deep learning in forensic sex estimation of virtual pelvic models from the Han population

引用

forensic sciences Research 2022年第3期7卷 540-549页

作者： Yongjie Cao Yonggang Ma Xiaotong Yang Jian Xiong Yahui Wang Jianhua Zhang Zhiqiang Qin Yijiu Chen Duarte Nuno Vieira Feng Chen Ji Zhang Ping Huang Shanghai Key Laboratory of forensic medicine Shanghai Forensic Service PlatformAcademy of Forensic ScienceMinistry of JusticeShanghaiChina department of forensic medicine Nanjing Medical UniversityNanjingChina department of Medical Imaging 3201 Hospital of Xi’an Jiaotong University Health Science CenterHanzhongChina department of forensic Pathology Shanxi Medical UniversityTaiyuanChina department of forensic medicine Guizhou Medical UniversityGuiyangChina Institute of Legal medicine Faculty of MedicineUniversity of CoimbraCoimbraPortugal

Accurate sex estimation is crucial to determine the identity of human skeletal remains ***,we developed convolutional neural network(CNN)models for sex estimation on virtual hemi-pelvic regions,including the ventral pubis(VP),dorsal pubis(DP),greater sciatic notch(GSN),pelvic inlet(PI),ischium,and acetabulum from the Han population and compared these models with two experienced forensic anthropologists using morphological methods.A Computed Tomography(CT)dataset of 862 individuals was divided into the subgroups of training,validation,and testing,*** CT-based virtual hemi-pelvises from the training and validation groups were used to calibrate sex estimation models;and then a testing dataset was used to evaluate the performance of the trained models and two human experts on the sex estimation of specific pelvic regions in terms of overall accuracy,sensitivity,specificity,F1 score,and receiver operating characteristic(ROC)*** for the ischium and acetabulum,the CNN models trained with the VP,DP,GSN,and PI images achieved excellent results with all the prediction metrics over *** accuracies were superior to those of the two forensic anthropologists in the independent ***,the heatmap results confirmed that the trained CNN models were focused on traditional sexual anatomic traits for sex *** study demonstrates the potential of AI techniques based on the radiological dataset in sex estimation of virtual pelvic *** excellent sex estimation performance obtained by the CNN models indicates that this method is valuable to proceed with in prospective forensic trials.

关键词： forensic sciences forensic anthropology sex estimation pelvis deep learning convolutional neural network

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Validation of the Investigator 24plex QS Kit:a 6-dye multiplex PCR assay for forensic application in the Chinese Han population

引用

forensic sciences Research 2022年第2期7卷 172-180页

作者： Ruiyang Tao Chong Chen Xiang Sheng Ruocheng Xia Xiaochun Zhang Jingyi Zhang Zihao Yang Suhua Zhang Chengtao Li Institute of forensic medicine West China School of Basic Medical Sciences&Forensic MedicineSichuan UniversityChengduChina Shanghai Key Laboratory of forensic medicine Shanghai Forensic Service PlatformAcademy of Forensic SciencesMinistry of JusticeShanghaiChina College of medicine and forensics Xi’an Jiaotong University Health Science CenterXi’anChina QIAGEN HildenGermany department of forensic medicine School of Basic Medical ScienceWenzhou Medical UniversityWenzhouChina department of forensic science Medical School of Soochow UniversitySuzhouChina

The Investigator 24plex QS Kit(QIAGEN,Hilden,Germany)is a 6-dye fluorescent chemistry short tandem repeat(STR)polymerase chain reaction(PCR)amplification system that simultaneously amplifies 20 of the expanded Combined DNA Index System(CODIS)core STR loci,SE33,DYS391,and the standard sex-determining locus,amelogenin,as well as two special internal performance quality sensor controls(QS1 and QS2),which are included in the primer mix to check the PCR *** study was designed to be a pilot evaluation of this STR-PCR kit in a Chinese Han population regarding the PCR conditions,sensitivity,precision,accuracy,repeatability,reproducibility,and concordance;tolerance to PCR inhibitors;applicability to real“forensic-type”samples;species specificity;mixture,balance and stutter analyses,and utility in a population *** exhaustive validation studies demonstrated that the Investigator 24plex QS system is accurate,sensitive and robust for STR *** addition,these genetic markers in the population data in our study indicated that they can also be useful for forensic identification and paternity testing in the Chinese Han population.

关键词： forensic sciences forensic genetics short tandem repeat(STR)typing Investigator 24plex QS Kit validation study

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A novel solution treatment and aging for powder bed fusion-laser beam Ti-6Al-2Sn-4Zr-6Mo alloy:Microstructural and mechanical characterization

引用

International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 414-424页

作者： Gianluca Pirro Alessandra Martucci Alessandro Morri Mariangela Lombardi Lorella Ceschini department of Industrial Engineering(DIN) Alma Mater Studiorum-University of BolognaBologna 40136Italy department of Applied science and Technology(DISAT) Politecnico di TorinoTorino TO 10129Italy

Ti-6Al-4Zr-2Sn-6Mo alloy is one of the most recent titanium alloys processed using powder bed fusion-laser beam(PBF-LB)*** alloy has the potential to replace Ti-6Al-4V in automotive and aerospace applications,given its superior mechanical properties,which are approximately 10%higher in terms of ultimate tensile strength(UTS)and yield strength after appropriate heat *** as-built conditions,the alloy is characterized by the presence of soft orthorhombicα″martensite,necessitating a postprocessing heat treatment to decompose this phase and enhance the mechanical properties of the ***,PBFed Ti6246 components undergo an annealing process that transforms theα″martensite into anα-βlamellar *** primary objective of this research was to develop a solution treatment and aging(STA)heat treatment tailored to the unique microstructure produced by the additive manufacturing process to achieve an ultrafine bilamellar microstructure reinforced by precipitation *** study investigated the effects of various solution temperatures in theα-βfield(ranging from 800 to 875℃),cooling media(air and water),and aging time to determine the optimal heat treatment parameters for achieving the desired bilamellar *** each heat treatment condition,differentα-βmicrostructures were found,varying in terms of theα/βratio and the size of the primaryα-phase *** attention was given to how these factors were influenced by increases in solution temperature and how microhardness correlated with the percentage of the metastableβphase present after *** tests were performed on samples subjected to the most promising heat treatment parameters.A comparison with literature data revealed that the optimized STA treatment enhanced hardness and UTS by13%and 23%,respectively,compared with those of the annealed *** surface analyses were conducted to investigate fracture mechanisms.

关键词： powder bed fusion-laser beam titanium alloys heat treatments mechanical properties fractographic analysis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：