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Development of a Label-Free Colorimetric Aptasensor with Rationally Utilized Aptamer for Rapid Detection of Okadaic Acid

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Journal of Ocean University of China 2022年第2期21卷 400-408页

作者： YAN Xiaochen QI Xiaoyan ZHAO Yinglin LI Ling MA Rui WANG Lele WANG Sai MAO Xiangzhao College of food Science and Engineering Ocean University of ChinaQingdao 266003China Laboratory for Marine drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology Qingdao 266237China

Okadaic acid(OA)is a typical marine toxin with strong toxicity causing diarrheic shellfish poisoning(DSP).Aptamers show great advantages in toxin detection and attract increasing attentions in the field of food *** this study,a label-free col-orimetric aptasensor was constructed for visual and rapid detection of OA in *** exploit the binding capability of the anti-OA aptamer,the inherent molecular recognition mechanism of aptamer and OA was studied,based on molecular docking,fluorescent assay,and biolayer *** results showed that the stem-loop near the 3’terminal of the aptamer exhibit dominate binding *** on the revealed recognition information,the aptamer was thus rationally utilized and combined with AuNPs and cationic polymer polydiallyl dimethyl ammonium chloride(PDDA)for the development of the label-free colorimetric aptasensor,in which the 3’terminal was thoroughly exposed to *** aptasensor provided robust performance with a linear detection range of 100-1200 nmol L-1,a limit of detection of 41.30 nmol L-1,recovery rates of 91.6%-106.2%,as well as a high selectivity towards OA in shellfish *** whole detection process can be completed within 1 *** our best knowledge,this is the first time that the anti-OA aptamer was thoroughly studied,and a label-free colorimetric aptasensor was rationally designed in this *** study not only provides a rapid detection method for highly sensitive and specific detection of OA,but also serves as a reference for the design of efficient aptasensors in the future.

关键词： aptamer okadaic acid colorimetric aptasensor molecular docking fluorescent assay biolayer interferometry

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Dietary Supplementation with Sea Cucumber Saponins and Exercise Can Significantly Suppress Adipose Accumulation in Mice Fed with High-Fat Diet

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Journal of Ocean University of China 2021年第3期20卷 629-640页

作者： LI Rong MENG Jing SHI Haohao WANG Chengcheng LI Zhaojie XUE Changhu WANG Yuming ZHANG Tiantian College of food Science and Engineering Ocean University of ChinaQingdao 266003China Laboratory for Marine drugs and Bioproducts Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China Jining Center for food and Drug Control Jining 272025China

Dietary supplementation with sea cucumber saponins(SCS)and exercise have been confirmed to be effective in pre-venting the development of obesity and its related ***,the combined effectiveness of these interventions has not been ***,we studied whether the beneficial influences of exercise could be further enhanced by dietary supplementation with SCS in high-fat diet-fed KM(Kunming)*** were randomly divided into four groups,including the high-fat diet group(HF),the SCS group(HF-S),the exercise group(HF-E),and the combination of dietary SCS and exercise group(HF-S+E).There were eight mice in every *** results demonstrated that the combination of dietary SCS and exercise could synergistically reduce fat *** particular,white adipose tissue decreased by 63%in the HF-S+E group compared with that in the HF *** supplementation with exercise also improved peripheral markers,such as serum parameters and hepatic TG *** mechani-cal testing indicated that the combined effects of dietary SCS and exercise on inhibiting fat accumulation might be attributed to the inhibition of lipid synthesis in the liver and the activation of lipolysis in white adipose tissue to increase energy consumption.

关键词： exercise sea cucumber saponins fat accumulation lipogenesis lipolysis

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Quantitative Structure-activity Relationship Studies on the Antioxidant Activity and Gap Junctional Communication of Carotenoids

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Chinese Journal of Structural Chemistry 2010年第9期29卷 1362-1372页

作者：孙玉敬吴丹刘东红陈健初沈妍叶兴乾 department of food Science and Nutrition School of Biosystems Engineering and Food ScienceZhejiang University

The antioxidant and gap junctional communication（GJC） activities of carotenoids are known to be the two main anticancer *** structure-activity relationship（QSAR） models of the two activities were developed using stepwise regression and multilayer perceptron neural network based on the calculated descriptors of quantum *** results showed that the significant molecular descriptor related to the antioxidant activity of carotenoids was the HOMO-LUMO energy gap（EHL） and the molecular descriptor related to the GJC was the lowest unoccupied molecular orbital energy（ELUMO）.The two models of antioxidant activity both showed good predictive power,but the predictive power of the neural network QSAR model of antioxidant activity was *** addition,the two GJC models have similar,moderate predictive *** possible mechanisms of antioxidant activity and GJC of carotenoids were discussed.

关键词： carotenoids antioxidant activity gap junctional communication multilayer perceptron neural network quantitative structure-activity relationship

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Laminarin and Laminarin Oligosaccharides Originating from Brown Algae:Preparation,Biological Activities,and Potential Applications

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Journal of Ocean University of China 2021年第3期20卷 641-653页

作者： HUANG Yi JIANG Hong MAO Xiangzhao CI Fangfang College of food Science and Engineering Ocean University of ChinaQingdao 266003China Laboratory for Marine drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology Qingdao 266237China

Brown algae is one of the three major types of marine algae and includes approximately 2000 *** is widely dis-tributed in various seas around the *** algae contain a plethora of active substances,such as polysaccharides,polyphe-nols,omega-3 fatty acids,and ***,a type of storage carbohydrate found abundantly in brown algae,is mainly formed by glucose monomers linked byβ-1,3-glucosidic bonds and partialβ-1,6-glucosidic *** and laminarin oligo-saccharides,which contain 2-10 saccharide units,have extensive biological activities,such as antitumor,antioxidant,anti-inflam-matory,and prebiotic ***,both laminarin and laminarin oligosaccharides can be considered as ideal substrates for bioethanol production because they are composed of abundant glucose ***,brown algae-derived laminarin and lami-narin oligosaccharides have various potential applications in the food,medicine,cosmetics,and bioenergy *** paper reviews the preparation methods of laminarin and laminarin oligosaccharides,as well as their biological activities and potential applications.

关键词： brown algae laminarin laminarin oligosaccharides biological activities potential applications

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P_(luxI)mutants with different promoting period and their application for quorum sensing regulated protein expression

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food Science and Human Wellness 2023年第5期12卷 1841-1849页

作者： Zhuoning Cao Zhen Liu Guilin Zhang Xiangzhao Mao College of food Science and Engineering Ocean University of ChinaQingdao 266003China Laboratory for Marine drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology Qingdao 266237China

Quorum sensing(QS)system can dynamically control the expression of proteins along with the cell *** promoting period of QS system has been little focused on until *** this study,a self-induced dynamic regulated expression(SIDRE)system was constructed in Escherichia *** enable the system suitable for the expression of enzymes,promoter engineering was used to obtain P_(luxI)*** test the SIDRE system,alginate lyase AL493 and esterase Est7 were used as target protein for *** enzyme activity of alginate lyase and esterase reached 96.38%and 106.71%of the control strains containing the T7 *** high-density fermentation,the activity of alginate lyase expressed by the SIDRE system with P_(luxI)(T-38C)as promoter was 4.34-fold of that expressed by the T7 ***,the P_(luxI)mutants with different promoting periods and/or different strengths show great potential in both laboratory and industrial scale for protein expression.

关键词： Quorum sensing Promoter engineering Enzymes Alginate lyase Esterase High-density fermentation

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Properties and Anti-Ultraviolet Activity of Gallic Acid-Chitosan-Gelatin Mixed Gel

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Journal of Ocean University of China 2022年第1期21卷 204-212页

作者： WANG Wenjie XUE Changhu MAO Xiangzhao College of food Science and Engineering Ocean University of ChinaQingdao 266003China Laboratory for Marine drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology Qingdao 266200China

Hydrogel has high water content and structural similarity with natural extracellular *** it has been widely studied and applied in the field of *** order to further develop multifunctional hydrogels,we prepared mixed gels with antiultraviolet *** study found that the addition of polysaccharides and polyphenols was beneficial to the rheological,me-chanical properties,and biological activity of the ***(CS)could significantly improve the viscoelasticity,hardness,gel strength,thermal stability and crystallinity of ***,the addition of gallic acid(GA)could not only provide significant cross-linking effect,improve gel properties and microstructure,but also improve the UV resistance of the mixed gel.

关键词： gallic acid chitosan gelatin gel properties UV resistance

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The role of axon guidance molecules in the pathogenesis of epilepsy

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Neural Regeneration Research 2025年第5期20卷 1244-1257页

作者： Zheng Liu Chunhua Pan Hao Huang department of Neurology Affiliated Hospital of Zunyi Medical UniversityZunyiGuizhou ProvinceChina

Current treatments for epilepsy can only manage the symptoms of the condition but cannot alter the initial onset or halt the progression of the disease. Consequently, it is crucial to identify drugs that can target novel cellular and molecular mechanisms and mechanisms of action. Increasing evidence suggests that axon guidance molecules play a role in the structural and functional modifications of neural networks and that the dysregulation of these molecules is associated with epilepsy susceptibility. In this review, we discuss the essential role of axon guidance molecules in neuronal activity in patients with epilepsy as well as the impact of these molecules on synaptic plasticity and brain tissue remodeling. Furthermore, we examine the relationship between axon guidance molecules and neuroinflammation, as well as the structural changes in specific brain regions that contribute to the development of epilepsy. Ample evidence indicates that axon guidance molecules, including semaphorins and ephrins, play a fundamental role in guiding axon growth and the establishment of synaptic connections. Deviations in their expression or function can disrupt neuronal connections, ultimately leading to epileptic seizures. The remodeling of neural networks is a significant characteristic of epilepsy, with axon guidance molecules playing a role in the dynamic reorganization of neural circuits. This, in turn, affects synapse formation and elimination. Dysregulation of these molecules can upset the delicate balance between excitation and inhibition within a neural network, thereby increasing the risk of overexcitation and the development of epilepsy. Inflammatory signals can regulate the expression and function of axon guidance molecules, thus influencing axonal growth, axon orientation, and synaptic plasticity. The dysregulation of neuroinflammation can intensify neuronal dysfunction and contribute to the occurrence of epilepsy. This review delves into the mechanisms associated with

关键词： axon guidance drug-resistant epilepsy epilepsy nerve regeneration nervous system diseases neural pathways neuroinflammatory diseases neuronal plasticity neurons synaptic remodeling

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A high-throughput screening method of cell transformation assay in Bhas 42 cells and the research on genistein

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中国药理学与毒理学杂志 2013年第3期 576-577页

作者： WANG Ying ZHANG Joe GENG Xing-chao LI Bo WANG Xue National Center for Safety Evaluation of drugs National Institute for Food and Drug Control Roche Research and Early Development China

OBJECTIVE This study was conducted to establish a high-throughput screening method of cell transformation assay in Bhas 42 cells,aiming to shorten the experimental period and increase the level of objectivity of in vitro cell transformation assay in the detection of carcinogens and to study the transformation potential of *** ① Assay Establishment:Two cell transformation methods were established in our lab for the Bhas 42 cell transformation assay: traditional foci formation method and hydrogen peroxide treatment *** hydrogen peroxide treatment method,transformation was quantified by measuring survival transformed cells OD value at 450 nm using CCK-8 on Day 20 after a treatment of the cell culture for 24 h by hydrogen peroxide on Day *** culture was then fixed,dyed and the number of foci was counted to verify *** the traditional foci formation method,the cell culture was fixed and dyed on Day 21 after plating cells in 96-well plate as described previously.② Study of the transformation effect of genistein in Bhas 42 cells using the H2 O2 treatment ***,a dose range-finding assay was performed to determine genistein concentrations according to the growth of cell treated with genistein in initiation and promotion assays ***,a definitive cell transformation assay was performed using 5 concentrations of genistein in either initiation or promotion *** 96well plate was used for each compound concentration,and each transformation assay also included negative,positive and blank *** ① In the initiation assay with 3-MCA as an initiator using foci formation,the number of wells having transformed foci was 53 wells /plate in positive control and 13 wells /plate in negative *** the promotion assay with TPA as a promoter using foci formation method,the number of wells having transformed foci was 40 wells /plate in positive control and 18 wells /plate in negative *** proportion of wells with tr

关键词： Bhas 42 cell transformation promotive activity non-genotoxic carcinogens genistein

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Biotransformation of Shrimp Wastes by Bacillus subtilis OKF04 and Evaluation of Growth Promoting Effect in Crop Planting

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Journal of Ocean University of China 2023年第5期22卷 1383-1392页

作者： HU Zelin PAN Zhaoyang ZHAO Tianyu WANG Yongzhen SUN Jianan MAO Xiangzhao College of food Science and Engineering Ocean University of ChinaQingdao 266003China Laboratory for Marine drugs and Bioproducts Qingdao National Laboratory for Marine Science and TechnologyQingdao 266237China

In this study,we proposed a reliable and sustainable technique for the clean utilization of shrimp wastes,which can yield a solid inoculant of Bacillus subtilis OKF04 containing micronutrients at low cost without the risk of *** of the culture conditions revealed that the head of shrimp Litopenaus vannamei and the wheat bran acted as suitable substrates for the growth of *** *** 60%initial moisture content,30℃culture temperature,and 5%inoculation amount,followed by 48 hours of fermentation and 0.5%soluble starch added during the drying process(50℃for 6h),a solid *** OKF04 inoculant with a spore amount of 2.4×10^(10)CFU g^(-1)and a high amino acid content was *** solid *** OKF04 inoculant was applied to cultivate pakchoi under pot *** the result,of adding to,the size of stems and leaves,nutritional composition,and physiological activity of pakchoi were significantly(P<0.05)enhanced by solid *** OKF04 *** OKF04 also significantly(P<0.05)increased the soil’s nutrient content and improved its microbial ***,pakchoi cultivated with a low dose of solid *** OKF04 inoculant(0.05 g kg^(-1)soil)resulted in the best *** study provides a new method for the preparation of microbial inoculants with solid waste shrimp heads.

关键词： shrimp wastes Bacillus subtilis OKF04 inoculant solid state fermentation crop growth promotion

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Inhibitory effect of reuterin-producing Limosilactobacillus reuteri and edible alginate-konjac gum film against foodborne pathogens and spoilage microorganisms

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food Bioscience 2023年第2期52卷 912-920页

作者： F.J.Rodrigues M.F.Cedran J.L.Bicas H.H.Sato food Biochemistry Laboratory Department of Food Science and NutritionSchool of Food EngineeringUniversity of CampinasCampinasSPBrazil food Biotechnology Laboratory Department of Food Science and NutritionSchool of Food EngineeringUniversity of CampinasCampinasSPBrazil

The possible inhibitory effects against foodborne pathogens and spoilage microorganisms of two strains(DSM 20016 and DSM 17938)of reuterin-producing Limosilactobacillus reuteri,and an edible alginate-konjac gum film containing *** cells and in situ produced reuterin was *** in vitro antimicrobial screening study indicated that both *** strains and the edible film showed inhibitory effects against ***,*** and ***,and towards the fungi ***,***,***,and *** addition,*** DSM 20016 was able to produce reuterin in situ(46.67 mmol/L)in alginate-konjac gum film-forming solution through anaerobic glycerol ***,after the edible film production by extrusion technique,both *** and reuterin were successfully trapped(98 and 67.6%,respectively).Further studies must be conducted to detect the exact metabolites related to the inhibition effects against foodborne pathogens and spoilage microorganisms.

关键词： 3-Hydroxypropionaldehyde Lactobacillus reuteri Antimicrobial activity Edible film Konjac gum

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