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Journal of Environmental sciences 2024年第3期137卷 120-130页

作者： Sen Yan Wei Sun Sinuo Tian Zhiyuan Meng Jinling Diao Zhiqiang Zhou Li Li Wentao Zhu innovation center of Pesticide research Department of Applied ChemistryCollege of ScienceChina Agricultural UniversityBeijing 100193China College of plant Protection/Joint International research Laboratory of Agriculture&Agri-Product Safety Yangzhou UniversityYangzhou 225009China College of plant Protection Shanxi Agricultural UniversityTaiyuan 030031China

Several studies have confirmed that the health status of the paternal affects the health of the offspring,however,it remains unknown whether paternal exposure to pesticides affect the offspring health.Here,we used untargeted metabolomics and 16S rRNA sequencing technology,combined with tail suspension test and RT-qPCR to explore the effects of paternal exposure to nitenpyram on the neurotoxicity of offspring.Our results found that the paternal exposure to nitenpyram led to the offspring’s depressive-like behaviors,accompanied by the reduction of tryptophan content and the disorder of microbial abundance in the gut of the offspring.Further,we determined the expression of tryptophan metabolism-related genes tryptophanase(tnaA)and tryptophan hydroxylase 1(TpH1)in gut bacteria and colonic tissues.We found that tryptophan is metabolized to indoles rather than being absorbed into colonocytes,which coursed the reduce of tryptophan availability after nitenpyram exposure.In conclusion,our study deepens our understanding of the intergenerational toxic effects of pesticides.

关键词： Nitenpyram Depressive-like behaviors Gut microbiota Tryptophan metabolism

Effects of prebiotics on the fermentation of traditional suancai of Northeast China

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food science and Human Wellness 2024年第3期13卷 1358-1367页

作者： Mingwei Zhao Xinying Cao Yuzheng Wu Sibo Zou Zhigao Li Xinping Lin Chaofan Ji Liang Dong Sufang Zhang Chenxu Yu Huipeng Liang National Engineering research center of Seafood Collaborative Innovation Center of Provincial and Ministerial Co-construction for Deep ProcessingCollaborative Innovation Center of Seafood Deep ProcessingSchool of Food Science and TechnologyDalian Polytechnic UniversityDalian 116034China department of Agricultural and Biosystems Engineering Iowa State UniversityIA 50011USA

Suancai is a traditional fermented vegetable widely consumed in Northeast China.In this study,different prebiotics were used to improve the quality of suancai.Four prebiotics(inulin(INU),xylooligosaccharide(XO S),galactooligosaccharide(GOS),and stachyose(STA))were shown to reduce the pH value and increase the content of total titratable acidity(TTA)in suancai,while the contents of most organic acids were also increased.The addition of prebiotics had significant effects on the bacterial microbiota during the suancai fermentation process.All prebiotics were shown to contribute to the growth of Lactobacillus.The suancai sample with fructooligosaccharides(FOS)had the highest relative abundance of Lactobacillus.Besides,INU and XOS could increase the abundance of Weissella.To evaluate the quality of suancai fermented with prebiotics,profiles of volatile flavor compounds(VOCs)and free amino acids(FAA)were analyzed.The prebiotics affected the VOCs and FAA profiles via transforming the bacterial microbiota.In addition,the addition of prebiotics also changed the taste profiles of the suancai samples.This study is among the first attempts to reveal the effects of different prebiotics on suancai fermentation,and the findings provide a foundation to develop new ways for improving the quality of suancai.

关键词： Bacterial microbiota Lactic acid bacteria Flavor Amino acid Taste

New insights into protein–polysaccharide complex coacervation:Dynamics,molecular parameters,and applications

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Aggregate 2024年第1期5卷 148-166页

作者： Jiabao Zheng Paul Van der Meeren Weizheng Sun School of food science and Engineering South China University of TechnologyGuangzhouChina department of Green Chemistry and Technology Faculty of Bioscience EngineeringGhent UniversityGhentBelgium Overseas Expertise Introduction center for Discipline innovation of food Nutrition and Human Health(111 center) GuangzhouChina

For more than a decade,the discovery of liquid–liquid phase separation within living organisms has prompted colloid scientists to understand the connection between coacervate functionality,phase behavior,and dynamics at a multidisciplinary level.Although the protein–polysaccharide was the first system in which the coacervation phenomenon was discovered and is widely used in food systems,the phase state and relaxation dynamics of protein–polysaccharide complex coacervates(PPCC)have rarely been discussed previously.Consequently,this review aims to unravel the relationship between PPCC dynamics,thermodynamics,molecular architecture,applications,and phase states in past studies.Looking ahead,solving the way molecular architecture spreads to macro-functionality,that is,establishing the relationship between molecular architecture–dynamics–application,will catalyze novel advancements in PPCC research within the field of foods and biomaterials.

关键词： dynamics encapsulation liquid coacervate protein-polysaccharide complex coacervation solid precipitate

Alternative splicing of the PECTINESTERASE gene encoding a cell wall-degrading enzyme affects postharvest softening in grape

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Journal of Integrative Agriculture 2024年第3期23卷 863-875页

作者： Hainan Liu Maosong Pei Charles Ampomah-Dwamena Yaxin Shang Yihe Yu Tonglu Wei Qiaofang Shi Dalong Guo College of Horticulture and plant Protection Henan University of Science and TechnologyLuoyang 471000China Henan Engineering Technology research center of Quality Regulation and Control of Horticultural plants Luoyang 471000China The New Zealand Institute for plant&food research Limited(PFR) Auckland 1010New Zealand

The firmness of table grape berries is a crucial quality parameter. Despite extensive research on postharvest fruit softening, its precise molecular mechanisms remain elusive. To enhance our comprehension of the underlying molecular factors, we initially identified differentially expressed genes(DEGs) by comparing the transcriptomes of folic acid(FA)-treated and water-treated(CK) berries at different time points. We then analyzed the sequences to detect alternatively spliced(AS) genes associated with postharvest softening. A total of 2,559 DEGs were identified and categorized into four subclusters based on their expression patterns, with subcluster-4 genes exhibiting higher expression in the CK group compared with the FA treatment group. There were 1,045 AS-associated genes specific to FA-treated berries and 1,042 in the CK-treated berries, respectively. Gene Ontology(GO) annotation indicated that the AS-associated genes in CK-treated berries were predominantly enriched in cell wall metabolic processes,particularly cell wall degradation processes. Through a comparison between treatment-associated AS genes and subcluster-4 DEGs, we identified eight genes, including Pectinesterase 2(VvPE2, Vitvi15g00704), which encodes a cell wall-degrading enzyme and was predicted to undergo an A3SS event. The reverse transcription polymerase chain reaction further confirmed the presence of a truncated transcript variant of VvPE2 in the FA-treated berries.Our study provides a comprehensive analysis of AS events in postharvest grape berries using transcriptome sequencing and underscores the pivotal role of VvPE2 during the postharvest storage of grape berries.

关键词： grape postharvest softening folic acid alternative splicing Pectinesterase 2 alternative 3'splice site(A3SS)

Staying hungry:a roadmap to harnessing central regulators of symbiotic nitrogen fixation under fluctuating nitrogen availability

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aBIOTECH 2024年第1期5卷 107-113页

作者： Lijin Qiao Jieshun Lin Takuya Suzaki Pengbo Liang State Key Laboratory of plant Environmental Resilience College of Biological SciencesChina Agricultural UniversityBeijingChina MOA Key Laboratory of Soil Microbiology Rhizobium Research CenterChina Agricultural UniversityBeijingChina department of Molecular Biology and Genetics Aarhus UniversityAarhusDenmark Faculty of Life and Environmental sciences University of TsukubaTsukubaIbarakiJapan Tsukuba plant-innovation research center University of TsukubaTsukubaIbarakiJapan

Legumes have evolved specific inventions to enhance nitrogen(N)acquisition by establishing symbiotic interactions with N-fixing rhizobial bacteria.Because symbiotic N fixation is energetically costly,legumes have developed sophisticated mechanisms to ensure carbon-nitrogen balance,in a variable environment,both locally and at the whole plant level,by monitoring nodule number,nodule development,and nodular nitrogenase activity,as well as controlling nodule senescence.Studies of the autoregulation of nodulation and regulation of nodulation by nodule inception(NIN)and NIN-LIKE PROTEINs(NLPs)have provided great insights into the genetic mechanisms underlying the nitrateinduced regulation of root nodulation for adapting to N availability in the rhizosphere.However,many aspects of N-induced pleiotropic regulation remain to be fully explained,such as N-triggered senescence in mature nodules.Wang et al.determined that this process is governed by a transcriptional network regulated by NAC-type transcription factors.Characterization and dissection of these soybean nitrogenassociated NAPs(SNAPs)transcription factor-mastered networks have yielded a roadmap for exploring how legumes rewire nodule functions across a range of N levels,laying the foundation for enhancing the growth of N-deprived crops in agricultural settings.

关键词： Symbiotic nitrogen fixation N-adapted nodulation Nodule senescence NIN NLPs SNAPs

Endomembrane-biased dimerization of ABCG16 and ABCG25 transporters determines their substrate selectivity in ABA-regulated plant growth and stress responses

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Molecular plant 2024年第3期17卷 478-495页

作者： Yeling Zhou Yuzhu Wang Dong Zhang Jiansheng Liang institute of plant and food science Department of BiologySchool of Life SciencesSouthern University of Science and Technology(SUSTech)Shenzhen 518055China Key Laboratory of Molecular Design for plant Cell Factory of Guangdong Higher Education Institutes Southern University of Science and Technology(SUSTech)Shenzhen 518055China jiangsu Key Laboratory of Crop Genetics and Physiology/Co-lnnovation center for Modern Production Technology of Grain Crops Key Laboratory of Plant Functional Genomics of the Ministry of EducationYangzhou UniversityYangzhou 225009China

ATP-binding cassette(ABC)transporters are integral membrane proteins that have evolved diverse func-tions fulfilled via the transport of various substrates.In Arabidopsis,the G subfamily of ABC proteins is particularly abundant and participates in multiple signaling pathways during plant development and stress responses.In this study,we revealed that two Arabidopsis ABCG transporters,ABCG16 and ABCG25,engage in ABA-mediated stress responses and early plant growth through endomembrane-specific dimerization-coupled transport of ABA and ABA-glucosyl ester(ABA-GE),respectively.We first revealed that ABCG16 contributes to osmotic stress tolerance via ABA signaling.More specifically,ABCG16 induces cellular ABA efflux in both yeast and plant cells.Using FRET analysis,we showed that ABCG16 forms oblig-atory homodimers for ABA export activity and that the plasma membrane-resident ABCG16 homodimers specifically respond to ABA,undergoing notable conformational changes.Furthermore,we demonstrated that ABCG16 heterodimerizes with ABCG25 at the endoplasmic reticulum(ER)membrane and facilitates the ER entry of ABA-GE in both Arabidopsis and tobacco cells.The specific responsiveness of the ABCG16-ABCG25 heterodimer to ABA-GE and the superior growth of their double mutant support an inhib-itory role of these twoABCGs in early seedling establishment via regulation of ABA-GE translocation across the ER membrane.Our endomembrane-specific analysis of the FRET signals derived from the homo-or heterodimerized ABcG complexes allowed us to link endomembrane-biased dimerization to the transloca-tion of distinct substrates by ABcG transporters,providing a prototypic framework for understanding the omnipotence of ABcG transporters in plant development and stress responses.

关键词： abscisic acid ABCG transporter dimerization endomembrane substrate selectivity

Alleviative effects of Bacillus coagulans strains on irritable bowel syndrome-unraveling strain specificity through physiological and genomic analysis

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food science and Human Wellness 2024年第4期13卷 1845-1855页

作者： Jiang Cao Leilei Yu Jianxin Zhao Hao Zhang Wei Chen Yuzheng Xue Qixiao Zhai State Key Laboratory of food science and Technology Jiangnan UniversityWuxi 214122China School of food science and Technology Jiangnan UniversityWuxi 214122China National Engineering research center for Functional food Jiangnan UniversityWuxi 214122China Wuxi Translational Medicine research center and Jiangsu Translational Medicine research Institute Wuxi Branch Wuxi 214122China department of Gastroenterology Affiliated Hospital of Jiangnan UniversityWuxi 214122China

The high intraspecies heterogeneity of Baciillus coagulans leads to significant phenotypic differences among different strains.Thus,6 B.coagulans strains were tested in the present study using an irritable bowel syndrome(IBS)animal model to determine whether the IBS-alleviating effects of B.coagulans strains are strain-specific.The results of this study showed that the ingestion of B.coagulans GBI-30,6086,and B.coagulans CCFM1041 significantly alleviated IBS symptoms in mice.In contrast,other B.coagulans strains showed no or limited alleviating effects on IBS symptoms.According to our experimental results,the two main common features of these strains were as follows:1)The resistance of vegetative cells to bile salts,and 2)ability to synthesize specific lipids and secondary metabolites.Screening strains based on these two indicators may greatly reduce costs and provide a basis for mining new functional B.coagulans strains.Our results also suggest that administration of B.coagulans could significantly regulate microbiota dysbiosis in animal models.Moreover,the close relationships between the gut microbiota,gut microbiota metabolites,and IBS were further confirmed in this study.

关键词： Baciillus coagulans Irritable bowel syndrome Resistance to bile salts Comparative genomic analysis Gut microbiota

SlBEL11 regulates flavonoid biosynthesis,thus fine-tuning auxin efflux to prevent premature fruit drop in tomato

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Journal of Integrative plant Biology 2024年第4期66卷 749-770页

作者： Xiufen Dong Xianfeng Liu Lina Cheng Ruizhen Li Siqi Ge Sai Wang Yue Cai Yang Liu Sida Meng Cai-Zhong Jiang Chun-Lin Shi Tianlai Li Daqi Fu Mingfang Qi Tao Xu College of Horticulture Shenyang Agricultural UniversityShenyang 110866China Collaborative innovation center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province College of Horticulture ScienceZhejiang A&F UniversityHangzhou 311300China Key Laboratory for Quality and Safety Control of Subtropical Fruits and Vegetables Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang ProvinceMinistry of Agriculture and Rural AffairsCollege of Horticulture ScienceZhejiang A&F UniversityHangzhou 311300China Key Laboratory of Protected Horticulture of Ministry of Education Shenyang 110866China Crops Pathology and Genetic research Unit United States Department of Agriculture Agricultural Research ServiceWashingtonDC 20250USA department of plant sciences University of CaliforniaDavisCA 95616USA ANGENOVO Oslo 0753Norway Laboratory of Fruit Biology College of Food Science and Nutritional EngineeringChina Agricultural UniversityBeijing 100083China

Auxin regulates flower and fruit abscission,but how developmental signals mediate auxin transport in abscission remains unclear.Here,we reveal the role of the transcription factor BEL1-LIKE HOMEODOMAIN11(SlBEL11)in regulating auxin transport during abscission in tomato(Solanum lycopersicum).SlBEL11 is highly expressed in the fruit abscission zone,and its expression increases during fruit development.Knockdown of SlBEL11 expression by RNA interference(RNAi)caused premature fruit drop at the breaker(Br)and 3d post-breaker(Br+3)stages of fruit development.Transcriptome and metabolome analysis of SlBEL11-RNAi lines revealed impaired flavonoid biosynthesis and decreased levels of most flavonoids,especially quercetin,which functions as an auxin transport inhibitor.This suggested that SlBEL11 prevents premature fruit abscission by modulating auxin efflux from fruits,which is crucial for the formation of an auxin response gradient.Indeed,quercetin treatment suppressed premature fruit drop in SlBEL11-RNAi plants.DNA affinity purification sequencing(DAP-seq)analysis indicated that SlBEL11 induced expression of the transcription factor gene SlMYB111 by directly binding to its promoter.Chromatin immunoprecipitation-quantitative polymerase chain reaction and electrophoretic mobility shift assay showed that S.lycopersicum MYELOBLASTOSIS VIRAL ONCOGENE HOMOLOG111(SlMYB111)induces the expression of the core flavonoid biosynthesis genes SlCHS1,SlCHI,SlF3H,and SlFLS by directly binding to their promoters.Our findings suggest that the SlBEL11-SlMYB111 module modulates flavonoid biosynthesis to fine-tune auxin efflux from fruits and thus maintain an auxin response gradient in the pedicel,thereby preventing premature fruit drop.

关键词： abscission auxin flavonoid SlBEL11 tomato

Unsynchronized butyrophilin molecules dictate cancer cell evasion of Vγ9Vδ2 T-cell killing

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Cellular & Molecular Immunology 2024年第4期21卷 362-373页

作者： Zeguang Wu Qiezhong Lamao Meichao Gu Xuanxuan Jin Ying Liu Feng Tian Ying Yu Pengfei Yuan Shuaixin Gao Thomas S.Fulford Adam P.Uldrich Catherine C.L Wong Wensheng Wei Biomedical Pioneering innovation center Peking-Tsinghua Center for Life SciencesPeking University Genome Editing Research CenterState Key Laboratory of Protein and Plant Gene ResearchSchool of Life SciencesPeking University100871BeijingChina EdiGene Inc. Life Science ParkChangping District102206BeijingChina center for Precision Medicine Multi-Omics research Peking University Health Science CenterPeking University100191BeijingChina department of Microbiology and Immunology at the Peter Doherty Institute for Infection and Immunity The University of MelbourneParkvilleVIC3010Australia Cancer Immunology Program Peter MacCallum Cancer CentreMelbourneVICAustralia State Key Laboratory for Complex Severe and Rare DiseasesClinical Research InstitutePeking Union Medical College HospitalChinese Academy of Medical Science&Peking Union Medical CollegePeking-Tsinghua Center for Life Sciences100871BeijingChina

Vγ9Vδ2 T cells are specialized effector cells that have gained prominence as immunotherapy agents due to their ability to target and kill cells with altered pyrophosphate metabolites.In our effort to understand how cancer cells evade the cell-killing activity of Vγ9Vδ2 T cells,we performed a comprehensive genome-scale CRISPR screening of cancer cells.We found that four molecules belonging to the butyrophilin(BTN)family,specifically BTN2A1,BTN3A1,BTN3A2,and BTN3A3,are critically important and play unique,nonoverlapping roles in facilitating the destruction of cancer cells by primary Vγ9Vδ2 T cells.The coordinated function of these BTN molecules was driven by synchronized gene expression,which was regulated by IFN-γsignaling and the RFX complex.Additionally,an enzyme called QPCTL was shown to play a key role in modifying the N-terminal glutamine of these BTN proteins and was found to be a crucial factor in Vγ9Vδ2 T cell killing of cancer cells.Through our research,we offer a detailed overview of the functional genomic mechanisms that underlie how cancer cells escape Vγ9Vδ2 T cells.Moreover,our findings shed light on the importance of the harmonized expression and function of gene family members in modulating T-cell activity.

关键词： Butyrophilin Vγ9Vδ2 T-cell Cancer-specific immune evasion Glutaminyl-peptide cyclotransferase-like Pyrophosphate metabolite Immunotherapy

Lactobacillus plantarum AR113 attenuates liver injury in D-galactose-induced aging mice via the inhibition of oxidative stress and endoplasmic reticulum stress

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food science and Human Wellness 2024年第2期13卷 885-897页

作者： Yongjun Xia Yujie Gong Xiangna Lin Yijin Yang Xin Song Guangqiang Wang Zhiqiang Xiong Yangyang Qian Zhuan Liao Hui Zhang Lianzhong Ai School of Health science and Engineering Shanghai Engineering Research Center of Food MicrobiologyUniversity of Shanghai for Science and TechnologyShanghai 200093China College of Life sciences Linyi UniversityLinyi 276000China department of Gastroenterol. Digestive Endoscopy CenterChanghai HospitalShanghai 200082China

Probiotics could effectively eliminate excess reactive oxygen species(ROS)generated during aging or lipid metabolism disorders,but their mechanism is unclear.The major purpose of this study was to investigate the mechanism of Lactiplantibacillus plantarun AR113 alleviating oxidative stress injury in the D-galactose induced aging mice.The result showed that pretreatment with L.plantarun AR113 significantly relieving H_(2)O_(2)induced cytotoxicity in HepG2 cells by maintain cell membrane integrity and increasing antioxidant enzyme activities.In D-galactose induced aging mice,L.plantarun AR113 could significantly attenuate liver damage and inflammatory infiltration by promoting endogenous glutathione(GSH)synthesis and activating the Nrf2/Keap1 signaling pathway in mice,and increasing the expression of regulated phaseⅡdetoxification enzymes and antioxidant enzymes.Further analysis shown that gavage of L.plantarun AR113 could significantly reduce the expression of G protein-coupled receptor 78(GPR78)and C/EBP homologous protein(CHOP)proteins,and promote the restoration of endoplasmic reticulum(ER)homeostasis,thereby activating cell anti-apoptotic pathways.These results were also confirmed in H_(2)O_(2)-treated HepG2 experiments.It indicated that L.plantarun AR113 could inhibit D-galactose-induced liver injury through dual inhibition of ER stress and oxidative stress.L.plantarun AR113 have good application potential in anti-aging and alleviating metabolic disorders.

关键词： Lactiplantibacillus plantarun AR113 HepG2 D-galactose Oxidative stress ER stress