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Effects of Coated Capillary Column, Derivatization, and Temperature Programming on the Identification of Carica papaya Seed Extract Composition Using GC/MS Analysis

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Journal of Analysis and Testing 2020年第1期4卷 23-34页

作者： Muhamad Shirwan Abdullah Sani Jamilah Bakar Russly Abdul Rahman Faridah Abas International Institute for Halal Research and Training Level 3KICT BuildingInternational Islamic University MalaysiaJalan Gombak53100 Kuala LumpurMalaysia Konsortium Institut Halal IPT malaysia Ministry of Higher EducationBlock E8Complex EFederal Government Administrative Centre62604 PutrajayaMalaysia Halal Product Research Institute Universiti Putra Malaysia43400 SerdangSelangorMalaysia department of food technology Faculty of Food Science and TechnologyUniversiti Putra Malaysia43400 SerdangSelangorMalaysia

Some of the common practices to assess the composition of plant extract,including Carica papaya seed extract(CPSE)are direct injection of the extract,compound separation using polyethylene glycol capillary column(DB-WAX),and non-linear-temperature programming(NLTP)of GC/MS *** study specifically compared the coating of capillary column,sample derivatization,and temperature programming of GC/MS to determine the composition of *** retention indices(RI)of the detected compounds were determined and compared to the reference *** particular,5%phenyl-95%methylpolysiloxane(HP-5MS)-,DB-WAX-,and biscyanopropyl polysiloxane(HP-88)-coated capillary columns were used to identify the composition of *** this study,HP-5MS column,which separated the highest number of compounds(26 compounds)from CPSE,was deemed as the most suitable *** GC/MS analysis of derivatized CPSE identified 21 compound groups,where fatty acids and fatty acid methyl esters served as the major compounds(80.23%),followed by these compounds in decreasing order:amides>nitriles>sterols>fatty aldehydes>organic acids.A stronger correlation determination between the carbon number and alkane retention time of linear-temperature programming(LTP)(R^(2)=0.9859)was found,as compared to its correlation determination with NLTP(R^(2)=0.9175),which exhibited an almost equal RI of LTP to the reference ***,GC/MS analysis for the derivatized CPSE using HP-5MS column separation and LTP is highly recommended.

关键词： Capillary column Carica papaya seed GC/MS Sample derivatization Temperature programming

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Quality and origin of mountain food products: the new European label as a strategy for sustainable development

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Journal of Mountain science 2019年第2期16卷 428-440页

作者： Deborah BENTIVOGLIO Sara SAVINI Adele FINCO Giorgia BUCCI Emanuele BOSELLI department of Agricultural Food and Environmental Sciences (D3A) Polytechnic University of Marche Chemical Safety department Experimental Station for the Food Preserving Industry (SSICA) faculty of science and technology Free University of Bolzano-Bozen

Mountain farming provides high-quality food products due to the peculiar characteristics of the raw materials combined with traditional processing conditions. However, these products and their intrinsic characteristics are not clearly recognized by consumers on the market. Nowadays, Protected Designation of Origin(PDO), Protected Geographical Indication(PGI) and Traditional Specialities Guaranteed(TSG) certifications include products originating in mountain areas, even if a large part of mountain foodstuff is not protected by these certifications and foods not made in mountain areas are often labeled as ―mountain food products‖. For this reason, the European Union has set a specific Regulation in 2012 to recognize and protect mountain food and to make the consumers safer about the origin information. The objective of this paper is twofold: firstly, it presents the recent aspects related to European legislation of mountain food product; secondly, it aims at reviewing the main features of nutritional quality and technology of dairy and meat products, as the most widespread mountain food products, and the main protocols used to evaluate the authenticity of these food products. The promotion of mountain food product through a specific label and the use of tracing methods, which are able to verify the authenticity of the origin of these products, may play a pivotal role in increasing the consumer‘s loyalty towards these products and could be a way to boost a sustainable development of these economically marginal rural areas. However, the analytical protocols developed so far(stable isotopes, macro and microelements, radionuclides, spectroscopic, molecular techniques, volatile substances profile, terpenes) are in many cases still at the experimental level due to the extreme variability of the mountain origin of the products to be tested.

关键词： Mountain products Label food technology Sustainable development Dairy products Meat products

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Preparation and characterization of asymmetric ultrafiltration membrane for effective recovery of proteases from surimi wash water

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Frontiers of Chemical science and Engineering 2012年第2期6卷 184-191页

作者： Nora'aini ALI Fadhilati HASSAN Sofiah HAMZAH department of Engineering science Faculty of Science and Technology University Malaysia Terengganu 21030 Kuala Terengganu Malaysia

The wash water generated from the surimi processing industry contains a large amount of proteases which are widely used in the food and biotechnology industries. Asymmetric polysulfone and polyethersulfone ultrafiltration （PSf-UF and PES-UF） membranes with three different polymer concentrations were screened for their abilities to recover proteases from surimi wash water. In- house fabricated membranes were prepared via a simple dry/wet phase inversion technique and were characterized in terms of permeability coefficient, membrane morphol- ogy and molecular weight cut-off （MWCO）. The ability of the UF membranes to remove commercial proteases was tested at various pressures （up to 10 bars）. The membrane with the best performance, 15 wt-% PSf-UF, was further tested with actual surimi wash water. The effect of the pH of the feed solution （4 to 8） in the pre-treatment stage was also evaluated to recover the highest amount of proteases. The highest retention of protease was 96% with a flux of 25.6 L/（mZ.h） which was achieved with the 15 wt-% PSf- UF membrane.

关键词： membrane ultrafiltration proteases surimi wash water

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Magnetic,thermal stability and dynamic mechanical properties of beta isotactic polypropylene/natural rubber blends reinforced by NiZn ferrite nanoparticles

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Defence technology（防务技术） 2019年第6期15卷 958-963页

作者： Lih-Jiun Yu Sahrim Hj Ahmad Ing Kong Mouad ATarawneh Shamsul Bahri Bin Abd Razak Elango Natarajan Chun Kit Ang department of Mechanical Engineering Faculty of EngineeringTechnology and Built EnvironmentUCSI UniversityNo 1Jalan Menara GadingUCSI Heights(Taman Connaught)Cheras56000Kuala LumpurMalaysia School of Applied Physics Faculty of Science and TechnologyUniversity Kebangsaan Malaysia43600BangiSelangor Darul EhsanMalaysia School of Engineering and Mathematical sciences La Trobe UniversityBundooraVIC3086Australia department of Physics College of ScienceAl-Hussein Bin Talal UniversityP.O Box 20Ma'anJordan department of food science and technology University Malaysia TerengganuKuala NerusTerengganuMalaysia

The dispersion of magnetic nanoparticles in matrix is crucial to ensure optimum performance of the *** difficulty level of achieving good dispersion is further increase when a multi-phases of matrix is present.A pre-coating technique of magnetic nanoparticles with polypropylene using ball-mill prior to melt-blending process was employed to prepare a multi-phases thermoplastic natural rubber *** effect of filler loading(2 wt%-10 wt%) on morphology,structure,magnetic properties,thermal stability and dynamic mechanical properties of the composites were *** was found that the NiZn ferrite nanoparticles act as nucleating agent to form beta isostatic polypropylene thermoplastic *** composites’ magnetic properties are directly dependent on the filler *** dispersion of magnetic fillers in polymer matrix plays role in affecting the magnetic properties and thermal *** preference of filler to locate at amorphous phase has distorted the chain orientation of natural rubber and ***,the polymorphism and crystallinity of the matrix varied as the filler loading increased,affecting the dynamic mechanical *** was found that 8 wt% NiZn nanocomposite exhibits highest E’ and tanδ,indicating the dynamic mechanical properties of NiZn nanocomposite are affected by β-phase degree.

关键词： Magnetic Thermoplastic natural rubber Nanoparticles Composites

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Chemical composition, antibacterial activity and antioxidant activity of Citrus bergamia essential oil: Molecular docking simulations

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food Bioscience 2022年第6期50卷 1468-1477页

作者： Mohammad Noshad Behrooz Alizadeh Behbahani Zahra Nikfarjam department of food science and technology Faculty of Animal Science and Food TechnologyAgricultural Sciences and Natural Resources University of KhuzestanMollasaniIran department of Physical&Computational Chemistry Chemistry and Chemical Engineering Research Center of IranTehranIran

Molecular docking is a simulation method based on bioinformatics, that evaluates the interaction between molecules (such as essential oil and target proteins), and predicts their binding modes and affinity via a computer platform. In this study, the chemical composition and antibacterial activity of Citrus bergamia essential oil (BEO) were investigated, and molecular docking was used to examine the mechanism of interaction between the main constituents of BEO and target proteins correlated with antibacterial activity and intracellular antioxidant mechanisms. Results showed the major ingredient of BEO was linalool, which accounted for 31.58% of the total components, followed by limonene (21.47%), octadecadienoic acid (12%), octadecenal (11%), ocimene (6.72%), pinene (2.73%), and hexadecanoic acid (1.71%). The antioxidant activity of BEO was 212 ± 2.65 μg/mL using the DPPH test, and 176.43 ± 1.59 μg/mL and 89.13 ± 1.37 μg/mL using the ABTS and β-carotene discoloration methods, respectively. BEO had a stronger antibacterial impact on Gram-positive bacteria (Staphylococcus aureus ATCC 6538) than on Gram-negative bacteria (Enterobacter aerogenes ATCC 13048). Finally, after analyzing the results related to the docking the three compounds, Octadecadienoic acid, Hexadecanoic acid, and 13-Octadecenal, have been reported to be effective compounds with high potency against a large number of selected receptors. Based on the results, BEO can be used as a new antibacterial agent in the pharmaceutical and food industries.

关键词： Natural essential oil Antibacterial activity Gas chromatography-mass spectrometry Molecular docking

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Expression of structural genes related to anthocyanin biosynthesis of Vitis amurensis

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Journal of Forestry Research 2016年第3期27卷 647-657页

作者： Quan Zhao Fei He Malcolm J.Reeves Qiu-Hong Pan Chang-Qing Duan Jun Wang Traditional Chinese Medicine department Jilin Agricultural Science and Technology College Center for Viticulture and Enology College of Food Science & Nutritional Engineering China Agricultural University faculty of Applied science Business and ComputingEastern Institute of Technology

This research was designed to assess the changes in anthocyanin content in grape skins of Vitis amurensis and to explore m RNA transcriptions of 11 structural genes（PAL,CHS3, CHI1, F3H2, F30 H, F3050 H, DFR, LDOX, UFGT,OMT and GST） related to anthocyanin biosynthesis during grape berry development, by the use of HPLC-MS/MS and real-time Q-PCR analysis. Accumulation of anthocyanins began at veraison, continued throughout the later berry development and reached a peak at maturity. Veraison is the time when the berries turn from green to purple. Expression of PAL, CHI1, and LDOX were up-regulated from 2 to4 weeks after flowering（WAF）, down-regulated from6 WAF to veraison, whereas DFR was up-regulated at8 WAF, and then up-regulated from veraison to ***3, F3050 H, UFGT, GST, and OMT were down-regulated from 2 WAF to veraison, and then up-regulated from veraison to maturity. The transcriptional expressions of the11 structural genes also showed positive correlations with the anthocyanin content from veraison to maturity. Positive correlations were also observed between OMT transcriptional level and the content of methoxyl-anthocyanins, and between F3050 H transcriptional level and the content of delphinidin anthocyanins. F3H2 and F30 H expression was up-regulated at 2 WAF. F3H2 expression was down-regulated from 4 WAF to veraison and then up-regulated again from veraison to maturity. F30 H expression was down-regulated at 4 WAF and then up-regulated again from 6 WAF to maturity. F30 H transcriptional level was correlated positively with the cyanidin anthocyanin concentration from veraison to maturity. These results indicate that the onset of anthocyanin synthesis during berry development coincides with a coordinated increase in the expression of a number of genes in the anthocyanin biosynthetic pathway.

关键词： Anthocyanins Gene expression HPLC-MS/MS Real-time Q-PCR Vitis amurensis

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Thyme oil inclusion levels in a rabbit ration: Evaluation of productive performance, carcass criteria and meat quality under hot environmental conditions

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Animal Nutrition 2018年第4期4卷 410-416页

作者： Ahmed A.A.Abdel-Wareth Eman M.M.Taha Karl-Heinz Südekum Jayant Lohakare department of Animal and Poultry Production Faculty of Agriculture South Valley University department of food science and technology Faculty of Agriculture South Valley University Institute of Animal science University of Bonn department of Agriculture-Animal science University of Arkansas at Pine Bluff

The aim of this study was to determine the impact of thyme essential oil supplementation in rabbit rations on performance, carcass criteria, and meat quality under hot environmental conditions. A total of 75, 4-week-old Californian male rabbits were assigned to 5 dietary treatments until 12 weeks of *** rabbits were reared in an open house system(38C average ambient temperature and 26% to 35%relative humidity). Treatments were as follows: unsupplemented standard ration, negative control(CON); standard ration supplemented with 1.50 g/kg olive oil as carrier, positive control(POS);POS t 50 mg/kg thyme oil(TO1); POS t 100 mg/kg thyme oil(TO2); and POS t 150 mg/kg thyme oil(TO3). Dietary thyme oil up to 150 mg/kg improved(P < 0.001) feed intake and growth performance in comparison to CON. The highest average daily gain and most efficient feed conversion ratio(linear,P < 0.001 and quadratic, P < 0.001) were found for TO1 followed by TO2, TO3 and POS, *** incorporation of thyme oil improved(P < 0.001) carcass criteria and decreased(P < 0.001) perirenal and scapular fat without any side effects on internal organs. Notably, the water holding capacity of rabbit meat was greater(P < 0.001), and the lipid oxidation was lower(P < 0.01) in rabbits fed treated rations compared with CON. Differences were also recorded in oxymyoglobin and metmyoglobin contents in rabbit meat among treatments. In conclusion, thyme oil of a specified composition and to be added to a rabbit ration up to 100 mg/kg using 1.50 g/kg olive oil as a carrier can be used as an efficient feed additive for improving productive performance of rabbits under hot environmental conditions.

关键词： Antioxidant Essential oil Meat characteristics Nutrition Production

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The effects of Lippia javanica dietary inclusion on growth performance,carcass characteristics and fatty acid profiles of broiler chickens

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Animal Nutrition 2016年第3期2卷 160-167页

作者： David A.Mpofu Upenyu Marume Victor Mlambo Arno Hugo department of Animal science Faculty of Agriculture Science and TechnologyNorth-West University food Security and Safety Niche Area Faculty of Agriculture Science and TechnologyNorth-West University department of Microbial Biochemical and Food BiotechnologyUniversity of Free State

This study was conducted to determine the effect of inclusion of fever tea(Lippia javanica) leaf meal in broiler diets on growth performance, carcass characteristics and fatty acid(FA) profiles over a 42-day feeding period. One hundred and eighty, one-day-old, broiler chicks were randomly allocated to the following four treatments: 1) negative control(commercial broiler diet only [Negcontrol]); 2) positive control(commercial broiler diet + prophylactic antibiotics [Poscontrol]); 3) commercial broiler diet without prophylactic antibiotics + 5 g of L. javanica per kg of feed(Ljav5) and 4) commercial broiler diet without prophylactic antibiotics + 12 g of L. javanica per kg of feed(Ljav12). Body weights(BW) and feed intake(FI) were recorded weekly and used to calculate feed conversion ratio(FCR) and average daily weight gain(ADG). At the end of the trial(day 42),all chickens were slaughtered at a local commercial abattoir for assessment of carcass characteristics and FA profiles of meat. The broilers fed L. javanica had significantly(P < 0.05) lower FI compared with the other two groups. However,the broilers in the Poscontrol and Ljav5 treatment groups had higher(P < 0.05) ADG, lower FCR and higher slaughter weights. L. javanica inclusion had no effect on the breast weight, thigh weight, carcass weight, and dressing percentage of the broilers. Most of the n-3 FA were not affected by diets except for the docosapentaenoic, which was found to be higher(P < 0.05) in the Ljav12 treatment group and the lowest in the Negcontrol. The broilers in the Negcontrol and Poscontrol groups had higher(P < 0.05) total saturated fatty acids(SFA). On the contrary, the L. javanica fed broilers had higher(P < 0.05) total polyunsaturated fatty acids(PUFA), total n-3 FA and PUFA:SFA ratio and also had significantly lower n-6:n-3 ratios compared with the other two treatment groups. No differences were observed with regards to total monounsaturated fatty acids(MUFA) and total n-6 FA. Overall, t

关键词： Fever tea Feed conversion ratio Average daily gain Carcass weight Fatty acids

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Citrus peel extract and powder attenuate hypercholesterolemia and hyperglycemia using rodent experimental modeling

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Asian Pacific Journal of Tropical Biomedicine 2017年第10期7卷 870-880页

作者： Humaira Ashraf Masood Sadiq Butt Muhammad Jawad Iqbal Hafiz Ansar Rasul Suleria National Institute of food science & technology Faculty of FoodNutrition & Home SciencesUniversity of Agriculture UQ School of Medicine Translational Research InstituteThe University of Queensland department of food NutritionDietetics & HealthKansas State University

Objective: To investigate hypocholesterolemic and hypoglycemic potential of citrus peel extract and powder using rodent experimental ***: Considering the fact, rat feeding trial was carried out for a period of 56 d to access the prophylaxis of citrus peel flavonoids by employing normal(study Ⅰ),hyperglycemic(study Ⅱ) and hypercholesterolemic(study Ⅲ) rats. Each study was further divided into three groups to ensure the provision of selected diets, i.e.,control, functional and nutraceutical diets. Each study was further divided into three groups to ensure the provision of selected diets, i.e., control, functional and nutraceutical ***: Declining trend for total cholesterol was observed in all studies with maximum reduction(8.55%) in rat group fed on nutraceutical diet in study Ⅲ. Likewise, levels of low density lipoproteins and triglycerides reduced 11.39% and 7.89% respectively in hypercholesterolemic rats. Moreover, nutraceutical diet alleviated the sera glucose level by 8.96% in study Ⅱ.Conclusions: Conclusively, inclusion of citrus peel bioflavonoids in dietary therapies is a promising strategy to modulate lipidemic and glycemic attributes without imparting any deleterious effect on hematological parameters.

关键词： Functional foods Nutraceutical Citrus powder Hypercholesterolemia Hyperglycemia Efficacy study

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Production of nitrite-free frankfurter-type sausages by combiningε-polylysine with beetroot extracts:An assessment of microbial,physicochemical,and sensory properties

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food Bioscience 2022年第5期49卷 746-754页

作者： Ali Ayaseh Kazem Alirezalu Milad Yaghoubi Zahra Razmjouei Shima Jafarzadeh Krystian Marszałek Amin Mousavi Khaneghah department of food science and technology Faculty of AgricultureUniversity of TabrizTabrizIran department of food science and technology Ahar Faculty of Agriculture and Natural ResourcesUniversity of TabrizTabrizIran School of Engineering Edith Cowan UniversityJoondalupWA6027Australia department of Fruit and Vegetable Product technology Prof.Wacław Dąbrowski Institute of Agricultural and Food Biotechnology-State Research Institute36 Rakowiecka St.02-532WarsawPoland department of General food technology and Nutrition Institute of Food Technology and NutritionCollege of Natural ScienceUniversity of RzeszowRzeszowPoland

The current study investigated the utilization ofε-polylysine(ε-PL)and beetroot extract(BE)as suitable nitrite substitutes in frankfurter-type sausages packaged in vacuum polyethylene *** sample’s technological,safety,antimicrobial,and organoleptic attributes were analyzed during 30 days of storage at 4℃.Compared with controls,the sausages containingϵ-PL with BE had no significant differences in the water activity and proximate *** results showed that the T5 and T4 samples had the highest residual nitrite and total phenolic contents at day ***ε-PL and BE into frankfurter-type sausages prevented the increase of pH and maintained samples’pink color and texture consistency during storage.T3 and T4 samples could effectively delay the growth of total viable count,coliform,Clostridium perfringens,mold,and yeasts within acceptable limitations during refrigerated storage.

关键词： Frankfurter-type sausage Beetroot extract ε-polylysine Nitrite Quality Sensory properties

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