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Behavioral Abnormality along with NMDAR-related CREB Suppression in Rat Hippocampus after Shortwave Exposure

Biomedical and Environmental Sciences 2019年第3期32卷 189-198页

作者： YU Chao BAI Yan Xin XU Xin Ping GAO Ya Bing HAO Yan Hui WANG Hui TAN Sheng Zhi LI Wen Chao ZHANG Jing YAO Bin Wei DONG Ji ZHAO Li PENG Rui Yun department of experimental Pathology Beijing Institute of Radiation Medicine

Objective To estimate the detrimental effects of shortwave exposure on rat hippocampal structure and function and explore the underlying mechanisms. Methods One hundred Wistar rats were randomly divided into four groups(25 rats per group) and exposed to 27 MHz continuous shortwave at a power density of 5, 10, or 30 m W/cm^2 for 6 min once only or underwent sham exposure for the control. The spatial learning and memory, electroencephalogram(EEG), hippocampal structure and Nissl bodies were analysed. Furthermore, the expressions of N-methyl-D-aspartate receptor(NMDAR) subunits(NR1, NR2 A, and NR2 B), c AMP responsive element-binding protein(CREB) and phosphorylated CREB(p-CREB) in hippocampal tissue were analysed on 1, 7, and 14 days after exposure. Results The rats in the 10 and 30 m W/cm^2 groups had poor learning and memory, disrupted EEG oscillations, and injured hippocampal structures, including hippocampal neurons degeneration, mitochondria cavitation and blood capillaries swelling. The Nissl body content was also reduced in the exposure groups. Moreover, the hippocampal tissue in the 30 m W/cm^2 group had increased expressions of NR2 A and NR2 B and decreased levels of CREB and p-CREB. Conclusion Shortwave exposure(27 MHz, with an average power density of 10 and 30 m W/cm^2) impaired rats' spatial learning and memory and caused a series of dose-dependent pathophysiological changes. Moreover, NMDAR-related CREB pathway suppression might be involved in shortwave-induced structural and functional impairments in the rat hippocampus.

关键词： Shortwave exposure Learning and memory Hippocampus NMDAR CREB

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Effects of Electromagnetic radiation on Autophagy and its Regulation

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Biomedical and Environmental Sciences 2018年第1期31卷 57-65页

作者： HAO Yan Hui ZHAO Li PENG Rui Yun department of experimental Pathology Beijing Institute of Radiation Medicine

With the ever increasing application of electronic technology, our exposure to artificial electromagnetic energy is also rapidly increasing. Electromagnetic radiation （EMR） is the fourth largest source of pollution,... 详细信息

关键词： Effects of Electromagnetic radiation Autophagy its Regulation

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How erastin assassinates cells by ferroptosis revealed

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Protein & Cell 2023年第2期14卷 84-86页

作者： Boyi Gan Department of Experimental Radiation Oncology The University of Texas MD Anderson Cancer CenterHoustonTX 77030USA

Ferroptosis represents an oxidative form of iron-dependent cell death resulting from unrestrained peroxidized phospholipids on cellular membranes(Jiang et al.,2021).In recent years,research interest in ferroptosis has increased steeply partly due to its high relevance to diverse diseases(such as cancer and neurodegener-ative diseases)and the enormous potential of ferroptosis induc-ers and inhibitors to treat these diseases(Jiang et al.,2021;Tang et al.,2021.

关键词： diseases death

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Dose-Dependent, Frequency-Dependent, and Cumulative Effects on Cardiomyocyte Injury and Autophagy of 2.856 GHz and 1.5 GHz Microwave in Wistar Rats

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Biomedical and Environmental Sciences 2022年第4期35卷 351-355页

作者： ZHANG Bo ZHANG Jing YAO Bin Wei XU Xin Ping WANG Hui ZHAO Li DONG Ji WANG Hao Yu TAN Sheng Zhi PENG Rui Yun department of experimental Pathology Beijing Institute of Radiation MedicineBeijing 100850China department of Pathology Beiya Orthopedic HospitalBeijing 100102China

With the in creasing use of microwave technology in wireless communication,medical research,and other fields,the risk of energy leakage and excessive human exposure requires more attention.Exposure to a certain frequency of microwave has been found to have harmful effects on multiple organs,including the heart[1-3],which is an important organ for blood circulation and any injury to which can cause a series of adverse consequences.

关键词： organs microwave consequences

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Dose-dependent Cardiac Dysfunction and Structural Damage in Rats after Shortwave radiation

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Biomedical and Environmental Sciences 2020年第8期33卷 603-613页

作者： ZHANG Jing YU Chao YAO Bin Wei WANG Hui ZHAO Li XU Xin Ping DONG Ji WANG Hao Yu HAO Yan Hui PENG Rui Yun department of experimental Pathology Institute of Radiation MedicineAcademy of Military Medical SciencesBeijing 100850China

Objective To detect the effects of shortwave radiation on dose-dependent cardiac structure and function in rats after radiation and to elucidate the mechanism of shortwave radiation induced cardiac injury to identify sensitive indicators and prophylactic treatment.Methods One hundred Wistar rats were either exposed to 27 MHz continuous shortwave at a power density of 5,10,and 30 mW/cm^2 for 6 min or undergone sham exposure for the control(the rats had to be placed in the exposure system with the same schedules as the exposed animals,but with an inactive antenna).The Ca^2+,glutamic oxaloacetic transaminase(AST),creatine kinase(CK)and lactate dehydrogenase(LDH)content in the peripheral serum of the rats were detected by an automatic blood biochemical analyser.The electrocardiogram(ECG)of standard lead II was recorded by a multi-channel physiological recording and analysis system.The cardiac structure of rats was observed by light and electron microscopy.Results The results showed that the 5,10,and 30 mW/cm^2 shortwave radiation caused a significant increased in the levels of Ca2+,AST,CK,and LDH in the peripheral serum of rats.The cardiac structure was damaged by radiation and showed a disordered arrangement of myocardial fibres,the cavitation and swelling of myocardial mitochondria.These injuries were most significant 7 d after radiation and were not restored until 28 d after radiation.Conclusion Shortwave radiation of 5,10,and 30 mW/cm^2 can damage rat cardiac function,including damage to the tissue structure and ultrastructure,especially at the level of the myocardial fibres and mitochondria.Shortwave radiation at 5,10,and 30 mW/cm^2 induced damage to rat heart function and structure with a dose-effect relationship,i.e.,the greater the radiation dose was,the more significant the damage was.

关键词： Shortwave Rat heart Function Structure Damage effect Dose dependence

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Biological and clinical aspects of HPV-related cancers

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Cancer Biology & Medicine 2020年第4期17卷 864-878页

作者： Klaudia Anna Szymonowicz Junjie Chen Department of Experimental Radiation Oncology The University of Texas MD Anderson Cancer CenterHoustonTX 77030USA

Cancer-related diseases represent the second overall cause of death worldwide.Human papilloma virus(HPV)is an infectious agent which is mainly sexually transmitted and may lead to HPV-associated cancers in both men and women.Almost all cervical cancers are HPV-associated,however,an increasing number of head and neck cancers(HNCs),especially oropharyngeal cancer,can be linked to HPV infection.Moreover,anogenital cancers,including vaginal,vulvar,penial,and anal cancers,represent a subset of HPVrelated cancers.Whereas testing and prevention of cervical cancer have significantly improved over past decades,anogenital cancers remain more difficult to confirm.Current clinical trials including patients with HPV-related cancers focus on finding proper testing for all HPV-associated cancers as well as improve the currently applied treatments.The HPV viral oncoproteins,E6 and E7,lead to degradation of,respectively,p53 and pRb resulting in entering the S phase without G1 arrest.These high-risk HPV viral oncogenes alter numerous cellular processes,including DNA repair,angiogenesis,and/or apoptosis,which eventually result in carcinogenesis.Additionally,a comprehensive analysis of gene expression and alteration among a panel of DNA double strand breaks(DSB)repair genes in HPV-negative and HPV-positive HNC cancers reveals differences pointing to HPV-dependent modifications of DNA repair processes in these cancers.In this review,we discuss the current knowledge regarding HPV-related cancers,current screening,and treatment options as well as DNA damage response-related biological aspects of the HPV infection and clinical trials.

关键词： Human papillomavirus(HPV) cervical cancer HNC DNA repair clinical trials cancer statistics

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Microwave-induced Apoptosis and Cytotoxicity of NK Cells through ERK1/2 Signaling

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Biomedical and Environmental Sciences 2017年第5期30卷 323-332页

作者： ZHAO Li LI Jing HAO Yan Hui GAO Ya Bing WANG Shui Ming ZHANG Jing DONG Ji ZHOU Hong Mei LIU Shu Chen PENG Rui Yun department of experimental Pathology Beijing Institute of Radiation Medicine department of Pathology General Hospital of Beijing Command of PLA

Objective To investigate microwave-induced morphological and functional injury of natural killer（NK） cells and uncover their mechanisms. Methods NK-92 cells were exposed to 10, 30, and 50 m W/cm^2 microwaves for 5 min. Ultrastructural changes, cellular apoptosis and cell cycle regulation were detected at 1 h and 24 h after exposure. Cytotoxic activity was assayed at 1 h after exposure, while perforin and NKG2 D expression were detected at 1 h, 6 h, and 12 h after exposure. To clarify the mechanisms, phosphorylated ERK（p-ERK） was detected at 1 h after exposure. Moreover, microwave-induced cellular apoptosis and cell cycle regulation were analyzed after blockade of ERK signaling by using U0126. Results Microwave-induced morphological and ultrastructural injury, dose-dependent apoptosis（P 〈 0.001） and cell cycle arrest（P 〈 0.001） were detected at 1 h after microwave exposure. Moreover, significant apoptosis was still detected at 24 h after 50 m W/cm^2 microwave exposure（P 〈 0.01）. In the 30 m W/cm^2 microwave exposure model, microwaves impaired the cytotoxic activity of NK-92 cells at 1 h and down regulated perforin protein both at 1 h and 6 h after exposure（P 〈 0.05）. Furthermore, p-ERK was down regulated at 1 h after exposure（P 〈 0.05）, while ERK blockade significantly promoted microwave-induced apoptosis（P 〈 0.05） and downregulation of perforin（P 〈 0.01）. Conclusion Microwave dose-dependently induced morphological and functional injury in NK-92 cells, possibly through ERK-mediated regulation of apoptosis and perforin expression.

关键词： Microwave Natural killer cells Cytotoxicity Apoptosis Cell cycle Perforin

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Mass spectrometry-based protein-protein interaction techniques and their applications in studies of DNA damage repair

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2021年第1期22卷 1-20页

作者： Zhen CHEN Junjie CHEN Department of Experimental Radiation Oncology The University of Texas MD Anderson Cancer CenterHoustonTX77030USA

Proteins are major functional units that are tightly connected to form complex and dynamic networks.These networks enable cells and organisms to operate properly and respond efficiently to environmental cues.Over the past decades,many biochemical methods have been developed to search for protein-binding partners in order to understand how protein networks are constructed and connected.At the same time,rapid development in proteomics and mass spectrometry(MS)techniques makes it possible to identify interacting proteins and build comprehensive protein-protein interaction networks.The resulting interactomes and networks have proven informative in the investigation of biological functions,such as in the field of DNA damage repair.In recent years,a number of proteins involved in DNA damage response and DNA repair pathways have been uncovered with MS-based protein-protein interaction studies.As the technologies for enriching associated proteins and MS become more sophisticated,the studies of protein-protein interactions are entering a new era.In this review,we summarize the strategies and recent developments for exploring protein-protein interaction.In addition,we discuss the application of these tools in the investigation of protein-protein interaction networks involved in DNA damage response and DNA repair.

关键词： Protein-protein interaction Interactome Proteomics Mass spectrometry DNA repair DNA damage response

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Dynamic Expression of Hyperpolarization-activated Cyclic Nucleotide-gated Cation Channel 4 Involved in Microwave Induced Pacemaker Cell Injuries

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Biomedical and Environmental Sciences 2015年第11期28卷 823-828页

作者： LIU Yan Qing ZHAO Li GAO Ya Bing DONG Ji WANG Hui YAO Bin Wei ZHOU Hong Mei WANG Shui Ming PENG Rui Yun department of experimental Pathology Beijing Institute of Radiation Medicine The Affiliated Hospital of Logistic College of Chinese People’s Armed Police Forces department of radiation Protection Beijing Institute of Radiation Medicine

To investigate the mechanisms of microwave induced pacemaker cell injuries, Wistar rats and the primary pacemaker cells of newborn Wistar rats were exposed to microwave at average power density of 50 mW/cm2. Slower spontaneous beating rate, intercellular Ca2＋ aggregation and cell membrane perforation were detected immediately after the exposure. Moreover, hyperpolarizationactivated cyclic nucleotide-gated cation channel 4 （HCN4） was down-regulated immediately after the exposure and up-regulated at 12 h after the exposure. In the sinoatrial node （SAN） of the rats,

关键词： HCN In node Dynamic Expression of Hyperpolarization-activated Cyclic Nucleotide-gated Cation Channel 4 Involved in Microwave Induced Pacemaker Cell Injuries SAN ISH Figure Ach AR

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Hepatopoietin Cn suppresses apoptosis of human hepatocellular carcinoma cells by up-regulating myeloid cell leukemia-1

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World Journal of Gastroenterology 2010年第2期16卷 193-200页

作者： Chu-Tse Wu department of experimental Hematology Beijing Institute of Radiation Medicine27 Taiping Road

AIM:To investigate the role of hepatopoietin Cn(HPPCn) in apoptosis of hepatocellular carcinoma(HCC)cells and its mechanism. METHODS:Two human HCC cell lines,SMMC7721 and HepG2,were used in this study.Immunostaining, Western blotting and enzyme linked immunosorbent assay were conducted to identify the expression of HPPCn and the existence of an autocrine loop of HPPCn/ HPPCn receptor in SMMC7721 and HepG2.Apoptotic cells were detected using fluorescein isothiocyanate (FITC)-conjugated Annexin V and propidium iodide.RESULTS:The HPPCn was highly expressed in human HCC cells and secreted into culture medium(CM). FITC-labeled recombinant human protein(rhHPPCn) could specifically bind to its receptor on HepaG2 cells. Treatment with 400 ng/mL rhHPPCn dramatically increased the viability of HCC-derived cells from 48.1% and 36.9%to 85.6%and 88.4%,respectively(P< 0.05).HPPCn silenced by small-interfering RNA reduced the expression and secretion of HPPCn and increased the apoptosis induced by trichostatin A.Additionally, HPPCn could up-regulate the expression of myeloid cell leukemia-1(Mcl-1)in HCC cells via mitogen-activated protein kinase(MAPK)and sphingosine kinase-1. CONCLUSION:HPPCn is a novel hepatic growth factor that can be secreted to CM and suppresses apoptosis of HCC cells by up-regulating Mcl-1 expression.

关键词： Hepatopoietin Cn Autocrine Hepatocellular carcinoma Apoptosis Myeloid cell leukemia-1

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