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Cloning and Characterization of a Salt Tolerance-Associated Gene Encoding Trehalose-6-Phosphate Synthase in Sweetpotato

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Journal of Integrative Agriculture 2014年第8期13卷 1651-1661页

作者： JIANG Tao ZHAI Hong WANG Fei-bing ZHOU Hua-nan SI Zeng-zhi HE Shao-zhen LIU Qing-chang Beijing Key Laboratory of crop Genetic Improvement/Laboratory of crop Heterosis and Utilization Ministry of Education/College of Agriculture and Biotechnology China Agricultural University

Trehalose plays an important role in metabolic regulation and abiotic stress tolerance in a variety of organisms. In plants, its biosynthesis is catalyzed by two key enzymes： trehalose-6-phosphate synthase（TPS） and trehalose-6-phosphate phosphatase（TPP）. In the present study, a TPS gene, named IbTPS, was first isolated from sweetpotato（Ipomoea batatas（L.） Lam.） cv. Lushu 3 by rapid amplification of cDNA ends（RACE）. The open reading frame（ORF） contained 2 580 nucleotides encoding 859 amino acids with a molecular weight of 97.433 kDa and an isoelectric point（pI） of 5.7. The deduced amino acid sequence showed high identities with TPS of other plants. Real-time quantitative PCR analysis revealed that the expression level of IbTPS gene was significantly higher in stems of Lushu 3 than in its leaves and roots. Subcellular localization analysis in onion epidermal cells indicated that IbTPS gene was located in the nucleus. Transgenic tobacco（cv. Wisconsin 38） plants over-expressing IbTPS gene exhibited significantly higher salt tolerance compared with the control plant. Trehalose and proline content was found to be significantly more accumulated in transgenic tobacco plants than in the wild-type and several stress tolerance related genes were up-regulated. These results suggest that IbTPS gene may enhance salt tolerance of plants by increasing the amount of treahalose and proline and regulating the expression of stress tolerance related genes.

关键词： cloning IbTPS gene Ipomoea batatas(L.) Lam. salt tolerance

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Variation and trends in dough rheological properties and flour quality in 330 Chinese wheat varieties

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The crop Journal 2014年第4期2卷 195-200页

作者： Xiushi Yang Li Wu Zhihua Zhu Guixing Ren Sancai Liu Institute of crop science Chinese Academy of Agricultural Sciences Faculty of biotechnology Industry Chengdu University

The objective of this study was to investigate variation and trends in dough rheological properties and flour quality traits in 330 Chinese wheat varieties. The dough rheological properties of development time(DT), stability time(ST), and farinograph quality number(FQN)were evaluated, as well as the flour quality traits of protein(PC), wet gluten content(WGC), and sedimentation value(SV). The coefficients of variation of DT(40.5%), ST(58.1%), and FQN(42.4%) were higher than those of PC(9.1%), WGC(10.1%), and SV(15.3%). Normal distributions were observed for the flour quality indices but not for the rheological parameters. SV was strongly correlated with the three rheological parameters and accordingly might be used as a primary indicator for dough rheological property evaluation. Our results showed that there has been marked improvement in dough rheological properties for Chinese wheat varieties released since 1986, while flour quality has remained stable.

关键词： Rheological property Flour quality Dough Genetic improvement Wheat

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Organic micro-pollutant removal in liquid-phase using carbonized silk cotton hull

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Journal of Environmental sciences 2008年第9期20卷 1046-1054页

作者： M Sathishkumar A R Binupriya D Kavitha R Selvakumar K K Sheema J G Choi department of Food science and Technology Institute of Agricultural Science and Technology Chonbuk National University department of Chemical Engineering Yonsei University department of biotechnology Bharathiar University department of Plant biology and biotechnology Government Arts College National Agricultural Products Quality Management Services

Phenolic compounds constitute one of the major pollutants in the modern world. Although many physical and chemical treatment technologies for their removal exist, most of them are economically not feasible. The present study was aimed at using silk cotton hull, a potent agricultural waste as an adsobent for removal of 2,4-dichlorophenol (2,4-DCP), which was used as a model phenolic compound. The process parameters were investigated and optimized conditions were determined. The equilibrium time was found to ...

关键词： silk cotton hull carbon adsorption 2 4-DCP temperature kinetic study

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Haplotype variation of Green Revolution gene Rht-D1 during wheat domestication and improvement

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Journal of Integrative Plant Biology 2014年第8期56卷 774-780页

作者： Chihong Zhang Lifeng Gao Jiaqiang Sun Jizeng Jia Zhenglong Ren Agronomy department Sichuan Agricultural University Institute of crop sciences Chinese Academy of Agricultural Sciences Key Laboratory of Crop Germplasm and Biotechnology Ministry of Agriculture Chengdu Institution of Biology theChinese Academy of Sciences

Green Revolution made a substantial contribution to wheat yields worldwide in the 196os and 197os. It is of great importance to analyze the haplotype variation of Rht-D1, the Green Revolution gene, during wheat （Triticum aestivum L.） domestication and breeding to understand its evolution and function in wheat breeding history. In this study, the Rht-D1 and its flanking regions were sequenced and single nucleotide polymorphisms were detected based on a panel of 45 accessions of Aegilops tauschii, 51 accessions of landraces and 8o accessions of commercial varieties. Genetic diversity in the wild accessions was much higher than that in the varieties and higher than that reported previously. Seven haplotypes （Hapl I to Hapl VII） of Rht-D1 were identified and their evolutionary relationships were proposed. In addition to the well-known Green Revolution allele Rht-Dlb, Hapl Vii （an allele Rht-Dlk） was identified in early breeding varieties, whichreduced plant height by 16%. The results suggested that Rht-Dlk had been used in breeding before the Green Revolution and made a great contribution to wheat production worldwide. Based on the breeding history and molecular evidence, we proposed that the wheat Green Revolution in China and International Maize and Wheat Improvement Center （CIMMYT） occurred independently.

关键词： Diversity haplotype Rht-D1 single nucleotidepolymorphism wheat

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Genome Editing Enables Next-Generation Hybrid Seed Production Technology

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Molecular Plant 2020年第9期13卷 1262-1269页

作者： Xiantao Qi Congsheng Zhang Jinjie Zhu Changlin Liu Changling Huang Xinhai Li Chuanxiao Xie nstitute of crop science Chinese Academy of Agricultural SciencesNational Key Facility for Crop Gene Resources and Genetic ImprovementBeijing 100081China Institute of biotechnology Institute Chinese Academy of Agricultural SciencesBeijing 100081China

The next-generation hybrid seed technology enables the successful production of sortable hybrid seeds from genic male sterile(GMS)lines and maintainers;however,it requires multiple laborious and complicated ***,we designed a simple next-generation hybrid seed production strategy that takes advantage of the CRISPR/Cas9 technology to create a Manipulated GMS Maintainer(MGM)system via a single *** this schema,the maize male fertility gene ZmMS26 was nullified by removal of its fifth exon using the CRISPR/Cas9 system on a vector,and a second vector carrying a functional ZmMS26 cDNA was co-transformed to restore *** second vector also contains a male gametophyte inactivation gene(ZmAA1)encoding maizeα-amylase driven by the pollen-specific promoter PG47 and an endosperm fluorescent marker(DsRED)driven by the barley endosperm aleurone-specific promoter *** derived single-copy hemizygous MGM lines bore a mutated MS26 gene,leading to complete male sterility but normal vegetative growth and grain *** MGM system could prevent genetic transmission of the MGM elements via male gametophytes,providing an efficient method for sorting maintainer seeds labeled by *** strategy can be extended to any GMS gene and to hybrid crops other than maize.

关键词： CRISPR/Cas9 genic male sterility manipulated maintainer ZmMS26 exon deletion

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Immobilization of nitrite oxidizing bacteria using biopolymeric chitosan media

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Journal of Environmental sciences 2013年第2期25卷 262-267页

作者： Pranee Lertsutthiwong Duangcheewan Boonpuak Wiboonluk Pungrasmi Sorawit Powtongsook Metallurgy and Materials science Research Institute Chulalongkorn University department of Environmental Engineering Faculty of EngineeringChulalongkorn University National Center for Genetic Engineering and biotechnology Center of Excellence for Marine biotechnology Department of Marine ScienceChulalongkorn University

The effects of chitosan characteristics including the degree of deacetylation, molecular weight, particle size, pH pretreatment and immobilization time on the immobilization of nitrite-oxidizing bacteria （NOB） on biopolymeric chitosan were investigated. Nitrite removal efficiency of immobilized NOB depended on the degree of deacetylation, particle size, pH pretreatment on the surface of chitosan and immobilization time. Scanning electron microscope characterization illustrated that the number of NOB cells attached to the surface of chitosan increased with an increment of immobilization time. The optimal condition for NOB immobilization on chitosan was achieved during a 24-hr immobilization period using chitosan with the degree of deacetylation larger than 80% and various particle size ranges between 1-5 mm at pH 6.5. In general, the NOB immobilized on chitosan flakes has a high potential to remove excess nitrite from wastewater and aquaculture systems.

关键词： chitosan immobilization nitrite-oxidizing bacteria pH adjustment

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Impact of thermal treatment and fermentation by lactic acid bacteria on sorghum metabolite changes, their antioxidant and antidiabetic activities

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Food Bioscience 2022年第1期45卷 284-292页

作者： Fred Kwame Ofosu Fazle Elahi Eric Banan-Mwine Daliri Sang-Ik Han Deog-Hwan Oh department of Food science and biotechnology College of Agriculture and Life SciencesKangwon National UniversityChuncheonGangwon-do24341South Korea department of Southern Area crop science NICS Upland Crop Breeding Res.Div.Gyeongsangnaam-do50424South Korea department of Biological Models Life Sciences CenterVilnius UniversityLithuania

This study evaluated the impact of thermal treatment and lactic acid bacteria fermentation of sorghum on the antioxidant,antidiabetic,nutritional and metabolite *** cooking and pressurized cooking improved the antioxidant capacity compared to *** of wet cooked(H95F)and pressurized cooked samples(H121F)further enhanced the ABTS and DPPH antioxidant capacity by 2.4 and 2.0 times;and 1.5 and 1.8 times higher than raw,*** acidilactici OHFR1 fermentation of sorghum M20 genotype after wet cooking at 60℃,80℃ and 95℃ significantly decreasedα-glucosidase inhibition to 39.1%,40.6% and 46.4%,respectively,compared to raw(51.6%).However,α-glucosidase inhibition significantly increased to 70.1% after pressurized cooking and ***,28 metabolites including phenolic compounds,amino acids and their derivatives,peptides and microbial metabolites were identified in sorghum M20 using UHPLC-Q-TOF-MS/MS non-targeted approach after *** to the raw sorghum sample,wet cooking and pressurized cooking resulted in 9 differential metabolites *** after wet and pressurized cooking resulted in 4 and 14 differential metabolites,respectively,compared to the *** findings confirm the potential of thermal treatment and fermentation to significantly modify sorghum bioactive components and their use as functional ingredients in the management of diabetes.

关键词： Whole sorghum grain Cooking Fermentation Antioxidant capacity Antidiabetic

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Genetic Diversity of Soybean and the Establishment of a Core Collection Focused on Resistance to Soybean Cyst Nematode

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Journal of Integrative Plant Biology 2006年第6期48卷 722-731页

作者： Yan-Song Ma Wen-Hui Wang Li-Xia Wang Feng-Ming Ma Pei-Wu Wang Ru-Zhen Chang Li-Juan Qiu Institute of crop science Chinese Academy of Agricultural Sciences National Key Facility of Crop Gene Resources and Genetic Improvement Crop Germplasm and Biotechnology Ministry of Agriculture Beijing 100081 China Jilin Agricultural University Changchun 130118 China Northeast of Agricultural University Harbin 150030 China

Soybean cyst nematode （SCN; Heterodera glycines） Is one of the most Important pests affecting soybean production. The best method of control of SCN is through the development of resistant cultlvars. However, limited progress has been made in soybean breeding In China because most modern cultlvars have no resistance to SCN. The distribution and phenotype of 432 immune or highly resistant Chinese accessions were surveyed and a primary core collection was selected as a representative sample for further analyses. Using evenly distributed simple sequence repeat markers, five selection methods were applied to the primary core collection and the optimal method was chosen to establish a core collection, which consisted of 28 accessions. These encompassed 70.8% of the ailelic variation present in the overall resistant collection. The 28 accessions differed from the reference resistant accessions at the genomlc level, Indicating that Chinese resistant accessions are distinct from known resistant accessions. This applied core collection provides a rational framework for undertaking diversity surveys, using genetic variation for the investigation of complex traits and for the discovery of novel traits.

关键词： applied core collection resistance simple sequence repeat marker soybean soybean cyst nematode

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Discrimination of individual seed viability by using the oxygen consumption technique and headspace-gas chromatography-ion mobility spectrometry

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Journal of Integrative Agriculture 2023年第3期22卷 727-737页

作者： TU Ke-ling YIN Yu-lin YANG Li-ming WANG Jian-hua SUN Qun department of Seed science and biotechnology College of Agronomy and BiotechnologyChina Agricultural University/The Innovation Center(Beijing)of Crop Seeds Whole-Process Technology ResearchMinistry of Agriculture and Rural Affairs/Beijing Key Laboratory of Crop Genetic ImprovementBeijing 100193P.R.China College of Arts and science of Hubei Normal University Huangshi 435109P.R.China College of science China Agricultural UniversityBeijing 100083P.R.China

Identifying and selecting high-quality seeds is crucial for improving crop *** purpose of this study was to improve the selection of crop seeds based on separating vital seeds from dead seeds,by predicting the potential germination ability of each seed,and thus improving seed *** methods of oxygen consumption (Q) of seeds and the headspace-gas chromatography-ion mobility spectrometry(HS-GC-IMS) were evaluated for identifying the viability of individual ***,the oxygen consumption technique showed clear differences among the values related to respiratory characteristics for seeds that were either vital or not,and the discrimination ability of final oxygen consumption(Q_(120)) was achieved not only in sweet corn seeds but also in pepper and wheat ***,Qtwas established as a new variable to shorten the measuring process in the Q2 (oxygen sensor) procedure,which was significantly related to the viability of individual *** minimize seed damage during measurement,the timing for viability evaluation was pinpointed at the 12,6 and 9 h for pepper,sweet corn,and wheat seeds based on the new variables concerning oxygen consumption (i.e.,Q_(12),Q_(6)and Q_(9),respectively).The accuracies of viability prediction were 91.9,97.7 and 96.2%,*** seeds were identified and hence discarded,leading to an enhancement in the quality of the seed lot as indicated by an increase in germination percentage,from 86.6,90.9,and 53.8%to all at 100%.We then used the HS-GC-IMS to determine the viability of individual sweet corn seeds,noting that corn seed has a heavier weight so the volatile gas components are more likely to be detected.A total of 48 chromatographic peaks were identified,among which 38 target compounds were characterized,including alcohols,aldehydes,acids and ***,there were no significant differences between the vital and dead seeds,due to the trace amount volatile composition differences among the individual ***

关键词： headspace-gas chromatography-ion mobility spectrometry oxygen consumption seed respiration sweet corn tomato wheat

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Molecular Identification and Expression Analysis of GhHYDRA1 Gene, a Homologue of HYDRA1 Gene From Upland Cotton (Gossypium hirsutum L.)

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Agricultural sciences in China 2011年第1期10卷 41-48页

作者： ZANG Zhen-le HU Ming-yu LI Xian-bi CHEN Kui-jun LIAO Peng XIAO Yue-hua HOU Lei PEI Yanand LUO Ming Key Laboratory of biotechnology and crop Quality Improvement Ministry of Agriculture/Biotechnology Research Center SouthwestUniversity Chongqing 400716 P.R.China

Beside as precursors of BRs biosynthesis, more and more evidences supported that phytosterols play an important role in plant growth and development. To investigate the effects of phytosterols on the fiber development of upland cotton （Gossypium hirsutum L.） and the molecular base of sterol regulating cotton fiber growth, a homologue of HYDRA1 was cloned from upland cotton （cv. Xuzhou 142） by screening cotton fiber EST database and contigging the candidate ESTs. The GhHYDRA1 encoded a polypeptide of 218 amino acid residues and the deduced amino acid sequences had high homology with the members of HYDRA1 in Populus trichocarpa, Solanum tuberosum, and Arabidopsis thaliana. Moreover, GhHYDRA1 had comparable transmembrane regions to AtHYDRA1 in sequence, length, order, and spacing, except for a C-terminal polylysine cluster. Quantitative real-time RT-PCR analysis revealed that the higher expression levels of GhHYDRA1 gene were detected in 6 to 12 DPA （days post anthesis） fibers, while the lower levels were observed in 0 DPA ovule （with fibers） and 16 to 18 DPA fibers. These results indicated that GhHYDRA1 is the homologue of HYDRA1 gene and plays a crucial role in fiber elongation. Furthermore, auxin and BL up-regulated the expression level of GhHYDRA1 while ABA and KT down-regulated the expression level of GhHYDRA1 in cotton ovule and fiber growth. The result suggested that phytosterols play a role in the interaction of plant hormones.

关键词： cotton fiber phytosterols brassinosteroids GhHYDRA1

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