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α-Mangostin and apigenin induced the necrotic death of BT474 breast cancer cells with autophagy and inflammation

Asian Pacific Journal of Tropical Biomedicine 2018年第11期8卷 519-526页

作者： Teeranai Ittiudomrak Songchan Puthong Tanapat Palaga Sittiruk Roytrakul Chanpen Chanchao Program in biotechnology Faculty of Science Chulalongkorn University Institute of biotechnology and Genetic Engineering Chulalongkorn University department of Microbiology Faculty of Science Chulalongkorn University National Center for Genetic Engineering and biotechnology (BIOTEC) National Science and Technology Development Agency department of Biology Faculty of Science Chulalongkorn University

Objective: To find new compounds in order to overcome the mainstay of metastatic breast cancer due to the adverse side effects from, and increasing resistance to, current chemotherapeutic agents. Methods: 毩-Mangostin and apigenin were reported in comparison to doxorubicin, a chemotherapeutic drug. Ductal carcinoma(BT474) cell line and nontumorigenic epithelial tissue from mammary gland(MCF-10 A) were used. Cell viability assessment was calculated by the standard 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method. Cell morphology was investigated by light microscopy. By flow cytometry analysis, programmed cell death was observed using annexin observed using propidium iodide st桋 and propidium iodide staining while cell-cycle arrest wasaining. Change in transcriptional expression was evaluated by real-time quantitative reverse transcription PCR. Results: In 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, the result revealed and apigenin were more cytotoxic to BT474 cells. Longer exposure times to 毩-mangostin enin caused more floating cells and a lower density of adhered cells wi毩-mangostin and apigth more vacuoles present in the colonies in BT474 only. 毩-Mangostin and apigenin caused necrosis in BT474 cells in a 24 h exposure, but a small amount of early apoptotic cells could also be detected at 24, 48 and 72 h exposure, whereas doxorubicin caused early apoptosis to BT474 cells at 24 h. Transcript expression and activity analysis supported caspase-3 was involved in the death of BT474 cells treated by all compounds. Moreover, 毩-mangostin and apigenin arrested the cellcycle at the G1-phase, but at the G2/M-phase by doxorubicin. All three compounds induced a change in transcript expression levels of inflammation-associated, proto-oncogene, autophagyassociated and apoptosis-associated genes. Conclusions: ntial new sources of chemotherapeuti毩-Mangostin and apigenin are worth investigating as potec agents for breast cancer treatment.

关键词： α-Mangostin Apigenin Breast cancer Cell cycle arrest Necrosis

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Sugar Starvation Enhances Leaf Senescence and Genes Involved in Sugar Signaling Pathways Regulate Early Leaf Senescence in Mutant Rice

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Rice science 2020年第3期27卷 201-214页

作者： LI Zhaowei ZHAO Qian CHENG Fangmin College of Life sciences Fujian Agriculture and Forestry UniversityFuzhou 350002China Institute of crop science College of Agriculture and BiotechnologyZhejiang UniversityHangzhou 310058China

To clarify the complex regulatory relationship between changes in sugar content and leaf senescence during the grain-filling stage of rice,genotype-dependent differences in sugar content and the temporal transcriptional patterns of genes involved in sugar signaling pathways were determined in mutant rice exhibiting early leaf senescence and its wild type Zhefu *** effects of exogenous glucose or sucrose on the senescence of detached leaves under dark conditions were also ***,soluble sugar,sucrose and fructose contents decreased,whereas electrolytic leakage and malondialdehyde levels increased in mutant leaves at the grain-filling *** results suggested that sugar starvation is positively correlated with the early leaf senescence of mutant *** leaf segments incubated in exogenous sugar solutions under dark conditions exhibited delayed *** high expression of Hxk1 in leaves of mutant plants at the initial grain-filling stage suggested that Hxk1 is involved in the hexose-sensing process at the early stage of leaf *** low expression levels of Hxk2 and Frk1 in the senescing leaves of mutant rice during the grain-filling stage are indicative of weakened hexose *** addition,the high expression levels of Su Sy1,Su Sy2 and Su Sy4 in leaves of mutant plants at the initial grain-filling stage are accompanied by the high transcript levels of SUT1,which favor sucrose translocation and remobilization from the early senescing leaves of mutant *** relatively reduced transcript levels of ch FBP,cy FBP,SPS1,SPS2 and SPS6 indicated that during the grain-filling stage,sucrose biosynthesis is weakened in the senescing leaves of mutant rice.

关键词： rice sugar starvation leaf senescence signaling pathway detached leaf segment grain-filling stage

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Assessment of different genetic distances in constructing cotton core subset by genotypic values

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Journal of Zhejiang University-science B(Biomedicine & biotechnology) 2008年第5期9卷 356-362页

作者： Jian-cheng WANG Jin HU Xin-xian HUANG Sheng-chun XU Seed science Center College of Agriculture and Biotechnology Zhejiang University Hangzhou 310029 China crop Research Institute Shandong Academy of Agricultural Sciences Jinan 250100 China

One hundred and sixty-eight genotypes of cotton from the same growing region were used as a germplasm group to study the validity of different genetic distances in constructing cotton core subset. Mixed linear model approach was employed to unbiasedly predict genotypic values of 20 traits for eliminating the environmental effect. Six commonly used genetic distances(Euclidean,standardized Euclidean,Mahalanobis,city block,cosine and correlation distances) combining four commonly used hierarchical cluster methods(single distance,complete distance,unweighted pair-group average and Ward's methods) were used in the least distance stepwise sampling(LDSS) method for constructing different core subsets. The analyses of variance(ANOVA) of different evaluating parameters showed that the validities of cosine and correlation distances were inferior to those of Euclidean,standardized Euclidean,Mahalanobis and city block distances. Standardized Euclidean distance was slightly more effective than Euclidean,Mahalanobis and city block distances. The principal analysis validated standardized Euclidean distance in the course of constructing practical core subsets. The covariance matrix of accessions might be ill-conditioned when Mahalanobis distance was used to calculate genetic distance at low sampling percentages,which led to bias in small-sized core subset construction. The standardized Euclidean distance is recommended in core subset construction with LDSS method.

关键词： Core subset Mixed linear model Least distance stepwise sampling (LDSS) method Standardized Euclidean distance Mahalanobis distance

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General and Exact Inbreeding Coefficient of Maize Synthetics Derived from Three-Way Line Hybrids

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Phyton-International Journal of Experimental Botany 2022年第1期91卷 33-43页

作者： Alejandro Ibarra-Sánchez Juan Enrique Rodríguez-Pérez Aureliano Peña-Lomelí Clemente Villanueva-Verduzco Jaime Sahagún-Castellanos crop science department Chapingo Autonomus UniversityChapingo56230México

Synthetic varieties(SVs)are populations generated by randomly mating their *** are a good alternative for low-input farmers who grow onions,maize,and other allogamous crops since the seed produced by a SV does not change from one generation to the *** SV progenitors are commonly pure lines,in this case a synthetic(Syn_(TC))whose parents are t three-way line crosses,a very common type of maize hybrid grown in Mexico,is *** aim was to develop a general and exact equation for the inbreeding coefficient of a Syn_(TC)eF_(m)^(f)Syn_(TC)T because of its relationship with the mean of economically important *** objective arose due to the need for a more advanced study in terms of determining whether F_(m)^(f)Syn_(TC)can be applied specifically and accurately for any number of parents(t),plants per parent(m)and inbreeding coefficient(IC)of the initial lines(F_(L)).A formula for the IC of the Syn_(TC)was derived that,given any values of F_(L)(0≤F_(L)≤1)and t,is specific for any value of m,not just for“large”numbers associated with the context in which the Hardy-Weinberg law is *** was found that F_(m)^(f)Syn_(TC)is very sensitive to changes in m when m is not greater than eight,after which it tends to stabilize very *** summary,unlike previously derived formulas,F_(m)^(f)Syn_(TC)is exact for any values of t,m and F_(L).

关键词： Genotypic array genotypic mean hybrid random mating Zea mays L

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The wheat MYB-related transcription factor TaMYB72 promotes flowering in rice

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Journal of Integrative Plant Biology 2016年第8期58卷 701-704页

作者： Lichao Zhang Guoxiang Liu Jizeng Jia Guangyao Zhao Chuan Xia Lina Zhang Fu Li Qiang Zhang Chunhao Dong Shuangcheng Gao Longzhi Han Xiuping Guo Xin Zhang Jinxia Wu Xu Liu Xiuying Kong Key Laboratory of crop Germplasm Resources and Utilization Ministry of AgricultureNational Key Facility for Crop Gene Resources and Genetic ImprovementInstitute of Crop ScienceChinese Academy of Agricultural SciencesBeijing 100081China biotechnology Research nstitute Chinese Academy of Agricultural SciencesBeijing 100081China department of Agriculture College of Agriculture SchoolHenan University of Science and TechnologyLuoyang 471023China

Through large-scale transformation analyses, Ta MYB72 was identified as a flowering time regulator in wheat. Ta MYB72 is a MYB family transcription factor localized to the nucleus. Three Ta MYB72 homologs,Ta MYB72-A, Ta MYB72-B and Ta MYB72-D, cloned from hexaploid wheat were mapped to the short arm of the group 6 chromosomes. Under the long-day conditions,over-expression of the Ta MYB72 in rice shortened the flowering time by approximately 12 d. Expression analyses suggest that Ta MYB72 may function through upregulation of florigen genes Hd3 a and RFT1.

关键词： Flowering transcriptional factor wheat

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SCFP, a novel fiber-specific promoter in cotton

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Chinese science Bulletin 2008年第17期53卷 2639-2645页

作者： HOU Lei LIU Hao LI JiaBao YANG Xia XIAO YueHua LUO Ming SONG ShuiQing YANG GuangWei PEI Yan Key Laboratory of biotechnology and crop Quality Improvement of the Ministry of Agriculture Biotechnology Research Center Southwest University Chongqing 400716 China

To investigate the expression pattern of GhSCFP which was isolated from cotton fiber cDNA library, a 1006 bp upstream fragment of the gene was cloned by chromosome walking and fused to GUS and GFP respectively. Histochemical GUS and GFP fluorescence analysis revealed that the expression of the report genes driven by the promoter sequence was detectable only in outer layer cells during the seed development in the transgentic tobaccos. In transgenic cotton, strong GUS activity was observed in spherical protrusions on 0 dpa (days post anthesis) ovule surface, and in the 2―36 dpa fiber cells, while no GUS signals were detected in the root, leaves, stem, corolla, anther and stigma. Our data demonstrated that GhSCFP upstream sequence is a cotton fiber-specific promoter and this promoter will be useful in the molecular research on fiber cell development and in cotton fiber improvements by genetic modification.

关键词：棉花纤维促进剂 SCFP 烟草植物生长调节剂

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Localization of calmodulin and calmodulin-like protein and their functions in biomineralization in P. fucata

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Progress in Natural science:Materials International 2008年第4期18卷 405-412页

作者： Zi Fang a, Zhenguang Yan a, Shuo Li a, Qin Wang a, Weizhong Cao a, Guangrui Xu a, Xunhao Xiong a, Liping Xie a,b, Rongqing Zhang a,b, a Institute of Marine biotechnology, department of Biological science and biotechnology, Tsinghua University, Beijing 100084, China b Protein science Laboratory of the Ministry of Education, Tsinghua University, Beijing 100084, China Institute of Marine biotechnology Department of Biological Science and Biotechnology Tsinghua University Beijing 100084 China Protein science Laboratory of the Ministry of Education Tsinghua University Beijing 100084 China

Calmodulin (CaM) and calmodulin-like protein (CaLP) are two proteins involved in biomineralization. Their localizations in Pinct- ada fucata mantle epithelia were studied by Western blot (WB) analysis of the nuclear/cytosol fraction of primary cultured P. fucata mantle cells and immunogold electron microscopy. The results showed a completely different distribution of these two proteins at the subcellular level. CaM was distributed throughout both the nucleus and cytoplasm of the mantle epithelium but CaLP was distributed only in the cytoplasm. The functions of these two proteins in biomineralization were investigated by shell regeneration. During this pro- cess, the expressions of CaM and CaLP were greatly enhanced in different organelles of the mantle epithelium. Overexpression of these two proteins and a mutant of calmodulin-like protein (M-CaLP) that lacks an extra C-terminal tail in MC3T3-E1 promoted the mRNA expression of osteopontin, a biomineralization marker for osteoblasts. All of the results indicated that CaM and CaLP have completely different distributions in the mantle epithelium and affect the biomineralization process at different levels. The extra C-terminal tail of CaLP is important for its functions in biomineralization in P. fucata.

关键词： Pinctada fucata Calmodulin Calmodulin-like protein Immunogold electron microscopy

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A field-deployable method for single and multiplex detection of DNA or RNA from pathogens using Cas12 and Cas13

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science China(Life sciences) 2022年第7期65卷 1456-1465页

作者： Lina Li Canxing Duan Jianfeng Weng Xiantao Qi Changlin Liu Xinhai Li Jinjie Zhu Chuanxiao Xie National Key Facility for crop Gene Resources and Genetic Improvement Institute of Crop ScienceChinese Academy of Agricultural Sciences(CAAS)Beijing 100081 China biotechnology Research Institute Chinese Academy of Agricultural Sciences(CAAS)Beijing 100081China

For some Cas nucleases,trans-cleavage activity triggered by CRISPR/Cas-mediated cis-cleavage upon target nucleic acid recognition has been explored for diagnostic *** single and multiplex nucleic acid-based detection is needed for crop pathogen management in ***,we harnessed and characterized RfxCas13d as an additional CRISPR/Cas nucleic acid detection *** systematically characterized AsCas12a,LbCas12a,LwaCas13a,and RfxCas13d combined with isothermal amplification to develop a CRISPR/Cas nucleic acid-based tool for single or multiplex pathogen *** data indicated that sufficient detection sensitivity was achieved with just a few copies of DNA/RNA targets as *** this tool,we successfully detected DNA from Fusarium graminearum and Fusarium verticillioides and RNA from rice black-streaked dwarf virus in crude extracts prepared in the *** method,from sample preparation to result readout,could be rapidly and easily deployed in the *** system could be extended to other crop pathogens,including those that currently lack a detection method and have metabolite profiles that make detection *** nucleic acid detection system could also be used for single-nucleotide polymorphism genotyping,transgene detection,and qualitative detection of gene expression in the field.

关键词： nucleic acid detection AsCas12a LbCas12a LwaCas13a RfxCas13d maize ear rot Fusarium head blight rice black-streaked dwarf virus(RBSDV)

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Structure and regulatory function of plant transcription factors

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Chinese science Bulletin 2001年第4期46卷 271-278页

作者： LIU Qiang, ZHANG Guiyou & CHEN Shouyidepartment of Biological science and biotechnology, Tsinghua Univorsity, Beijing 100084, China department of Biological science and biotechnology, State Laboratory of Biomembrane and Membrane biotechnology, Tsinghua University, Beijing 100084, China Institute of Genetics, Chinese Academy of sciences, Beijing 100101, China department of Biological science and biotechnology Tsinghua University Beijing China department of Biological science and biotechnology State Laboratory of Biomembrane and Membrane Biotechnology Tsinghua University Beijing China Institute of Genetics Chinese Academy of Sciences Beijing China

The expression of inducible genes in plants is regulated by specific transcription factors at the transcrip-tional level. A typical transcription factor usually contains a DNA-binding domain, a transcription regulation domain, a dimerization site and a nuclear localization domain. These functional domains define the characteristic, localization and regulatory role of a transcription factor. Transcription factors recognize and bind to specific cis-acting elements or interact with other proteins, and then activate or repress the transcription of target genes by their functional domains. In recent years, elucidation on the structure and function of transcription factors has become an important subject in plant molecular biology.

关键词： transcription factor DNA-binding domain cis-acting element.

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Characterization of Gh SERK2 and its expression associated with somatic embryogenesis and hormones level in Upland cotton

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Journal of Integrative Agriculture 2018年第3期17卷 517-529页

作者： LIU Zheng-jie ZHAO Yan-peng ZENG Ling-he ZHANG Yuan WANG Yu-mei HUA Jin-ping College of Agronomy and biotechnology China Agricultural University/Key Laboratory of Crop Heterosis and Utilization Ministry of Education/Beijing Key Laboratory of Crop Genetic Improvement United States department of Agriculture - Agricultural Research Service (USDA-ARS) Crop Genetics Research Unit Research Institute of Cash crop Hubei Academy of Agricultural Sciences

Somatic embryogenesis （SE） is one of the most important steps during regeneration of cotton, but the molecular mechanism of SE remains unclear. SOMATIC EMBRYOGENSIS RECEPTOR KINASE （SERK） gene is known to function in SE. A homolog GhSERK2 （accession number： JF430801） was cloned from Upland cotton and characterized for its functions in SE. GhSERK2 expressed in different tissues and showed higher expression level in floral organs than vegetative ones with the highest levels in ovule and anther. GhSERK2 expressed during SE with a high level at globular embryos stage. Upon treatment with indole-3-butytic acid （IBA）, the transcription level of GhSERK2 was induced and promoted SE subsequently. A 2-day treatment of 2,4-dichlorophenoxyacetic acid （2,4-D） induced the expression of GhSERK2, but treatments of 2,4-D for longer periods sharply inhibited the GhSERK2 transcription level of embryogenic callus （EC）. The levels of hormones, including 3-indoleacetic acid （IAA）, abscisic acid （ABA）, and brassinosteroid （BR）, were increased in the initial calli induced from the over-expression of GhSERK2 cotton. Our results indicated that GhSERK2 expression was associated with induction of SE and closely related to hormone levels during tissue culture in Upland cotton, and the gene might play an important role in regeneration of cotton.

关键词： GhSERK2 somatic embryogenesis hormone level expression pattern Upland cotton (Gossypium hirsutum L.)

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