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Xiangshao Granules Ameliorate Post-stroke Depression by Inhibiting Activation of Microglia and IDO1 Expression in Hippocampus and Prefrontal Cortex

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Chinese Journal of Integrative Medicine 2025年第1期31卷 28-38页

作者： LI Cheng-gang XU Lu-shan SUN Liang XU Yu-hao CAO Xiang ZHAO Chen-chen XIA Sheng-nan ZHANG Qing-xiu XU Yun department of Neurology Nanjing Drum Tower HospitalAffiliated Hospital of Medical SchoolNanjing UniversityNanjing210008China State Key Laboratory of Pharmaceutical biotechnology and Institute of Translational Medicine for Brain Critical Diseases Nanjing UniversityNanjing210008China Jiangsu Key Laboratory for Molecular Medicine Medical School of Nanjing UniversityNanjing210008China Nanjing Neurology Medical Center Nanjing210008China

Objective To investigate the therapeutic effect of Xiangshao Granules(XSG)on post-stroke depression(PSD)and explore the underlying *** Forty-three C57BL/6J mice were divided into 3 groups:sham(n=15),PSD+vehicle(n=14),and PSD+XSG(n=14)groups according to a random number *** PSD models were constructed using chronic unpredictable mild stress(CUMS)after middle cerebral artery occlusion(MCAO).The sham group only experienced the same surgical operation,but without MACO and CUMS *** XSG group received XSG(60 mg/kg per day)by gavage for 4 *** mice in the sham and vehicle groups were given the same volume of 0.9%saline at the same *** body weight and behavior tests including open field test,sucrose preference test,tail suspension test,and elevated plus-maze test,were used to validate the PSD mouse ***-time fluorescence quantitative polymerase chain reaction(RT-qPCR),enzyme-linked immunosorbent assay(ELISA),and immunofluorescence staining were used to evaluate the anti-inflammatory effects of *** potential molecular mechanisms were explored and verified through network pharmacology analysis,Nissl staining,Western blot,ELISA,and RT-qPCR,*** The body weight and behavior tests showed that MCAO combined with CUMS successfully established the PSD *** alleviated neuronal damage,reduced the expressions of pro-apoptotic proteins Caspase-3 and B-cell lymphoma-2(BCL-2)-associated X(BAX),and increased the expression of anti-apoptotic protein BCL-2 in PSD mice(P<0.05 or P<0.01).XSG inhibited microglial activation and the expressions of pro-inflammatory cytokines including tumor necrosis factor-α,interleukin(IL)-1β,and IL-6 via the toll-like receptor 4/nuclear factor kappa-B signaling pathway in PSD mice(P<0.05 or P<0.01).Furthermore,XSG decreased the expression of indoleamine 2,3-dioxygenase1(IDO1)and increased the concentration of 5-hydroxytryptamine in PSD mice(P<0.05 or P<0.01).Conclusion XSG could reverse

关键词： post-stroke depression Xiangshao Granules indoleamine 2,3-dioxygenase 1 Chinese medicine

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SPEI_(PM)-based research on drought impact on maize yield in North China Plain

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Journal of Integrative Agriculture 2015年第4期14卷 660-669页

作者： MING Bo GUO Yin-qiao TAO Hong-bin LIU Guang-zhou LI Shao-kun WANG Pu Institute of crop science Chinese Academy of Agricultural Sciences/Key Laboratory of Crop Physiology and EcologyMinistry of Agriculture College of Agriculture and biotechnology China Agricultural University

The calculation method of potential evapotranspiration（PET） was improved by adopting a more reliable PET estimate based on the Penman-Monteith equation into the standardized precipitation evapotranspiration index（SPEI） in this study（SPEI PM）. This improvement increased the applicability of SPEI in North China Plain（NCP）. The historic meteorological data during 1962–2011 were used to calculate SPEI PM. The detrended yields of maize from Hebei, Henan, Shandong, Beijing, and Tianjin provinces/cities of NCP were obtained by linear sliding average method. Then regression analysis was made to study the relationships between detrended yields and SPEI values. Different time scales were applied, and thus SPEI PM was mentioned as SPEI PMk-j（k=time scale, 1, 2, 3, 4,…, 24 mon; j=month, 1, 2, 3,..., 12）, among which SPEI PM3-8 reflected the water condition from June to August, a period of heavy precipitation and vigorous growth of maize in NCP. SPEI PM3-8 was highly correlated with detrended yield in this region, which can effectively evaluate the effect of drought on maize yield. Additionally, this relationship becomes more significant in recent 20 yr. The regression model based on the SPEI series explained 64.8% of the variability of the annual detrended yield in Beijing, 45.2% in Henan, 58.6% in Shandong, and 54.6% in Hebei. Moreover, when SPEI PM3-8 is in the range of –0.6 to 1.1, –0.9 to 0.8 and –0.8 to 2.3, the detrended yield increases in Shandong, Henan and Beijing. The yield increasing range was during normal water condition in Shandong and Henan, where precipitation was abundant. It indicated that the field management matched well with local water condition and thus allowed stable and high yield. Maize yield increase in these two provinces in the future can be realized by further improving water use efficiency and enhancing the stress resistance as well as yield stability. In Hebei and Beijing, the precipitation is less and thus the normal water condition cannot meet

关键词： North China Plain maize(Zea mays L.) drought climate-determined yield standardized precipitation evapotranspiration index

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Rice tissue-specific promoters and conditiondependent promoters for effective translational application

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Journal of Integrative Plant Biology 2015年第11期57卷 913-924页

作者： Hee-Jeong Jeong Ki-Hong Jung department of Plant Molecular Systems biotechnology and crop Biotech Institute Kyung Hee University Graduate School of biotechnology Kyung Hee University

Rice （Oryza sativa） is one of the most important staple food crops for more than half of the world＇s population. The demand is increasing for food security because of population growth and environmental challenges triggered by climate changes. This scenario has led to more interest in developing crops with greater productivity and sustainability. The process of genetic transformation, a major tool for crop improvement, utilizes promoters as one of its key elements. Those promoters are generally divided into three types： constitutive, spatiotemporal, and condition-dependent. Tran- scriptional control of a constitutive promoter often leads to reduced plant growth, due to a negative effect of accumu- lated molecules during cellular functions or energy consump- tion. To maximize the effect of a transgene on transgenic plants, it is better to use condition-dependent or tissue- specific promoters. However, until now, those types have not been as widely applied in crop biotechnology. In this review, we introduce and discuss four groups of tissue-specific promoters （5o promoters in total） and six groups of condition-dependent promoters （27 promoters）. These pro- moters can be utilized to fine-tune desirable agronomic traits and develop crops with tolerance to various stresses, enhanced nutritional value, and advanced productivity.

关键词： Condition-dependent promoters crop biotechnologyapplication genetic transformation rice tissue-specific promoters

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Production of a monoclonal antibody specific for high molecular weight glutenin subunits (HMW-GS) in wheat and its antigenic determinant

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science China(Life sciences) 2005年第1期48卷 89-96页

作者： WANG Hanqian ZHANG Xueyong WANG Hongmei PANG Binshuang Key Laboratory of crop Germplasm and biotechnology Ministry of AgricultureInstitute of Crop ScienceChinese Academy of Agricultural SciencesBeijing100081China

Wheat high molecular weight glutenin subunits(HMW-GS)1Bx14 and 1By15 isolated by preparative SDS-PAGE are used as antigen to immunize BALB/c *** inoculation of the antigen is *** intra-peritoneal injection is completed 3 days before fusion with myeloma cell(SP2/0)via *** fusion cells are selected by indirect enzyme-linked immuno-sorbent assay(ELISA).Positive hybrid cells are further verified three times by limit dilution of the culture cells.A hybridoma cell line is successfully *** monoclonal antibody belongs to IgG1 *** immunoblotting,the antibody binds to all HMW-GS of *** cultivars,but does not bind to other storage proteins in seeds of *** result is consisting with the high homology in amino acid sequences among the HMW glutenin subunits in *** antibody also binds to HMW-GS storage proteins in Aegilops squarrosa and ***(durum wheat).Furthermore,it also binds to HMW storage proteins in Secale cereale(rye),Hordeum vulgare(barley).However,it never binds seed storage proteins in other cereals such as maize,oat,rice,foxtail millet,sorghum *** antigen determinant recognized by the antibody has been located within hexapeptide[PGQGQQ]or/and nonapeptide[GYYPTSPQQ]in the central repetitive region of HMW-GS.

关键词： wheat HWM-GS monoclonal antibody antigenic determinant immunoblotting.

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Antioxidant and lipoxygenase inhibitory properties of a novel flavonoid from Pistacia chinensis Bunge and its molecular docking analysis

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Traditional Medicine Research 2025年第2期10卷 30-36页

作者： Abdur Rauf Zuneera Akram Naveed Muhammad Najla AlMasoud Taghrid Saad Alomar Saima Naz Abdul Wadood Chandni Hayat Marcello Iriti department of Chemistry University of SwabiAmbar 23561Pakistan department of Pharmacology Faculty of Pharmaceutical SciencesBaqai Medical UniversityKarachi 74600Pakistan department of Pharmacy Abdul Wali Khan University MardanMardan 23200Pakistan department of Chemistry College of SciencePrincess Nourah bint Abdulrahman UniversityRiyadh 11671Saudi Arabia department of biotechnology BKUC-Bacha Khan University CharsaddaCharsadda 24620Pakistan department of Biochemistry Abdul Wali Khan University MardanMardan 23200Pakistan department of Biomedical Surgical and Dental SciencesUniversity of MilanMilan 20129Italy National Interuniversity Consortium of Materials science and Technology(INSTM) Firenze 50121Italy

Background:Pistacia chinensis Bunge has been traditionally used to manage various conditions,including asthma,pain,inflammation,hepatoprotection,and *** study was conducted to investigate the antioxidant and anti-lipoxygenase(LOX)properties of the isolated compound 2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-4H-chromen-4-one from Pistacia ***:LOX assay and antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl(DPPH)assay were *** docking studies were conducted using a molecular operating ***:The LOX assay revealed significant inhibitory effects at 0.2µM concentration,with an IC50 value of 37.80µ*** antioxidant effect demonstrated dose-dependency across 5 to 100µg/mL concentrations,reaching 93.09%at 100µg/mL,comparable to ascorbic acid’s 95.43%*** docking studies highlighted strong interactions with the lipoxygenase enzyme,presenting an excellent docking score of-10.98 kcal/***:These findings provide valuable insights into Pistacia chinensis’chemical components and biological effects,reinforcing its traditional medicinal applications.

关键词： Pistacia chinensis Bunge antioxidant DPPH assay antilipoxygenase docking analysis

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Effects of maternal feeding of clofibrate on hepatic fatty acid metabolism in suckling piglet

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Journal of Animal science and biotechnology 2025年第1期16卷 424-440页

作者： Jinan Zhao Brandon Pike Feng Wang Lin Yang Paige Meisner Yanling Huang Jack Odle Xi Lin Laboratory of Developmental Nutrition Department of Animal ScienceNorth Carolina State UniversityRaleighNC 27695USA Sales department Zinpro CorporationEden PrairieMN 55344USA Extension Agent NC State UniversityN.C.Cooperative Extension-Northampton County Center9495 NC Highway 305PO Box 636JacksonNC 27845USA Key Laboratory of Animal science of State Ethnic Affairs Commission Southwest Minzu UniversityChengdu 610041People’s Republic of China

Background Energy deficiency is a leading cause of the high pre-weaning mortality of neonatal piglets in the swine ***,optimal energy metabolism is of crucial importance for improving the survivability of neonatal *** effective utilization of milk fat as primary energy is indispensably *** Pregnant sows(n=27)were randomly assigned into 3 *** treatment received a standard diet(3,265 kcal ME/kg)supplemented with either 0,0.25%or 0.5%clofibrate(w/w)from d 107 of gestation to d 7 of *** effects of maternal clofibrate on their milk fatty acid(FA)and performance of the piglets were *** evaluations were performed via measuring sow productive performance,milk FA composition,and hepatic FA oxida-tion of the piglets at birth and d 1,7,14 and 19 after *** Maternal supplementation of clofibrate had no effect on reproductive performance of the sows at farrowing and weaning(P>0.05).However,the mortality at weaning was reduced for piglets from sows with 0.25%of clofi-brate,and the average weekly(and daily)gain was higher in piglets from sows that received clofibrate than sows without clofibrate in the first week(P<0.0001).Maternal clofibrate increased percentage of milk C12:0 and C14:0 FAs but decreased C18:2 and n-6 polyunsaturated *** clofibrate also increased plasma ketone body levels and hepatic FA oxidation measured at the first day of birth,but the increase was not detected in piglets on d 7,14 or *** was not detected in milk collected from the clofibrate-treated *** percentage of FA oxidation decreased,and the percentage of FA esterification increased with increasing in postnatal *** carni-tine increased FA oxidation regardless of succinate dehydrogenase inhibition,and the increase had no effect on FA *** Maternal supplementation of clofibrate during late gestation and early lactation increases hepatic FA oxidative metabolism at birth and imp

关键词： Clofibrate Hepatic fatty acid oxidation and metabolism Newborn pigs Postnatal age PPARα

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Cryopreservation of bovine sperm causes single‑strand DNA breaks that are localized in the toroidal regions of chromatin

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Journal of Animal science and biotechnology 2025年第1期16卷 92-106页

作者： Jordi Ribas‑Maynou Rodrigo Muiño Carolina Tamargo Marc Yeste biotechnology of Animal and Human Reproduction(TechnoSperm) Institute of Food and Agricultural TechnologyUniversity of GironaS17003 GironaSpain Unit of Cell Biology Department of BiologyFaculty of SciencesUniversity of GironaS17003 GironaSpain Present Address:Unit of Cell Biology and Medical Genetics Department of Cell BiologyPhysiology and ImmunologyAutonomous University of BarcelonaS08193 BellaterraSpain department of Animal Pathology Faculty of Veterinary MedicineUniversity of Santiago de CompostelaS15705 LugoSpain department of Animal Selection and Reproduction The Regional Agri-Food Research and Development Service of Asturias(SERIDA)S33394 GijónSpain Catalan Institution for Research and Advanced Studies(ICREA) S08010 BarcelonaSpain

Background Sperm cryopreservation is widely used in the cattle industry,as it allows for disassociating the localiza-tion of sires and the collection of semen from the timing of artificial *** freeze-thawing is known to impair sperm DNA integrity,whether the damage induced consists of single-(SSB)or double-strand breaks(DSB)has not been *** addition,no previous study has addressed if DNA breaks preferentially reside in specific genome regions such as those forming the toroid linker regions,or are rather spread throughout the regions linked to *** main aim of the present work,therefore,was to elucidate the type and localization of the DNA damage generated by cryopreservation and to evaluate its impact on artificial insemination outcomes in *** The incidence of SSB and DSB was evaluated in 12 ejaculates before and after cryopreservation with the Comet assay,and the localization of the DNA breaks was assessed using pulsed-field gel electrophoresis(PFGE).Before cryopreservation,the incidence of SSB was 10.99%±4.62%and involved 20.56%±3.04%of sperm cells,whereas these figures significantly(P0.990)before and after cryopreservation(before:incidence of 13.91%±1.75%of sperm DNA affecting 56.04%±12.49%of sperm cells;after:incidence of 13.55%±1.55%of sperm DNA involving 53.36%±11.00%of sperm cells).Moreover,PFGE revealed that the percentage of sperm DNA fragments whose length was shorter than a toroid(<31.5 kb)was greater(P<0.0001)after(27.00%±4.26%)than before freeze-thawing(15.57%±4.53%).These differences indicated that the DNA breaks induced by cryopreservation affect the regions condensed in protamines,which are structured in *** the other hand,in vivo fertility rates were associated to the incidence of SSB and DSB in frozen-thawed sperm(P=0.032 and P=0.005),but not w

关键词： Cattle DNA damage Fertility Sperm Toroid linker regions

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A monoclonal antibody against Lates calcarifer vitellogenin and a competitive ELISA to evaluate vitellogenin induction after exposure to xenoestrogen

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Journal of Environmental sciences 2019年第1期31卷 325-333页

作者： Witchuda Prasatkaew Phochit Nanthanawat Nanthika Khongchareonporn Sutin Kingtong Environmental science program Faculty of Science Burapha University department of biotechnology Faculty of Science Burapha University The Institute of biotechnology and Genetic Engineering Chulalongkorn University department of Biology Faculty of Science Burapha University

A monoclonal antibody specific to sea bass(Lates calcarifer) vitellogenin(VTG) was developed,for use as a tool for monitoring endocrine disrupting chemicals(EDCs). VTG was induced in sea bass by intramuscular injection of 17β-estradiol(E_2: 2 mg/kg) every three days. Blood was collected three days after the last injection. Plasma VTG was then purified by chromatography in hydroxyapatite and a sephacryl-S300 column. Characterizations of purified VTG were done by phospholipoglycoprotein staining on a native-PAGE with confirmation by mass spectrometry(LC-MS/MS). Antibody was raised in mice by injection of purified VTG. After monoclonal antibody production, the hybridoma clone No. 41(MAb-sea bass VTG 41)was selected and developed for quantification of VTG by competitive enzyme-linked immunosorbent assay(ELISA). The ELISA method was sensitive with a detection limit of VTG 40 ng/mL. MAb-sea bass VTG 41 was specific to VTG from E_2-treated sea bass and others EDCs(Nonylphenol, Benzo[a]pyrene and CdCl_2). Moreover, cross-reactivity was also found in E_2-treated coral grouper(Epinephelus corallicola). The ELISA method obtained from this work can be further applied for the assessment of EDCs in Thailand and Southeast Asia's aquatic environment.

关键词： Monoclonal antibody Vitellogenin Biomarker Endocrine disrupting chemicals(EDCs) ELISA

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A telomere-to-telomere genome assembly of the cultivated peanut

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Molecular Plant 2025年第1期18卷 5-8页

作者： Xiaobo Wang Ziqi Sun Feiyan Qi Zhiyuan Zhou Pei Du Lei Shi Wenzhao Dong Bingyan Huang Suoyi Han Stefano Pavan Meng Zhang Mengjie Cui Jing Xu Hua Liu Li Qin Zhongxin Zhang Xiaodong Dai Wei Gao Lijuan Miao Ruifang Zhao Juan Wang Mengmeng Wang Chenyang Zhi Yaojun Hu Huanhuan Zhao Linjie Chen Xiaodong Jin Yanhui Sun Zheng Zheng Xinyou Zhang The Shennong Laboratory Zhengzhou 450002China Institute of crop Molecular Breeding Henan Academy of Agricultural SciencesZhengzhou 450002China Henan Provincial Key Laboratory for Genetic Improvement of Oil crops Zhengzhou 450002China National Innovation Centre for Bio-breeding Industry Xinxiang 453000China department of Soil Plant and Food SciencesUniversity of Bari Aldo MoroBari 70126Italy

Dear Editor,Cultivated peanut(peanut in short)(Arachis hypogaea,2n=4x=40,genome type AABB)is a legume crop serving globally as a crucial source of edible oil and *** peanut reference genome sequences obtained for the cultivars"Tifrunner,""Shitouqi,"and"Fuhuasheng"were assembled or patched using third-generation sequencing technologies and significantly contributed to advancements in peanut genetics and breeding(Bertioli et al.,2019;Chen et al.,2019;Zhuang et al.,2019).However,unresolved gaps persist in these assemblies,particularly in regions of biological importance such as centromeres and telomeres(Supplemental Figure 1),hindering comprehensive exploration of their functional and evolutionary significance.

关键词： breeding cultivar Figure

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Concentration and retention of ascorbic acid,carotenoids,and sugars in fresh-cut lettuce in modified atmosphere packaging

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Horticultural Plant Journal 2025年第1期11卷 303-313页

作者： Jinita Sthapit Kandel Ivan Simko Ryan J.Hayes Beiquan Mou U.S.department of Agriculture Agricultural Research ServiceSam Farr United States Crop Improvement and Protection Research Center1636 E.Alisal StreetSalinasCA93905USA U.S.department of Agriculture Agricultural Research ServiceForage Seed and Cereal Research Unit3450 SW Campus WayCorvallisOR97331USA U.S.department of Agriculture Agricultural Research ServiceSunflower and Plant Biology Research1616 Albrecht Blvd NFargoND58102USA

Fresh-cut lettuce is widely used in ready-to-eat salads sold in modified atmosphere packages (MAP).Even in MAP,fresh-cut lettuce has short shelf life that results in loss of *** cultivars exhibit genetic variation for shelf life in MAP,but their variation for nutrient retention is not *** accessions were evaluated for initial content of ascorbic acid (AsA),carotenoids,and sugars and their retention in *** with high content and/or good retention of one or more nutrients were *** romaine accession ‘Floricos’ had high levels of all the three *** with relatively high retention of all the three nutrients were ‘Salinas 88’,‘Siskiyou’,‘Solar’,SM09A,‘Romance’,and ‘Green Towers’.Romaine cultivars,‘Balady Barrage’,‘Green Towers’,and ‘Darkland’ had relatively high initial levels of all tested nutrients and good rate of their *** was no clear correlation between initial AsA/carotene concentrations and their retention rates,suggesting that besides content,retention of nutrients should also be a breeding target in a lettuce nutritional improvement *** analyses with the Pearson's correlation coefficient determined a negative relationship between tissue deterioration(AUDePS) and retention of all tested nutrients[r of-0.52 (P<0.0001) for AsA,-0.27 (P<0.01) for total carotene,and-0.59 (P<0.0001) for total sugars],suggesting that an increase in tissue deterioration intensifies nutrient ***-sense heritability (H^(2)) across the experiments was0.15 for AsA,0.23 for total carotene,and 0.50 for total *** of germplasm with high nutrient content,extended shelf life and good nutrient retention provides valuable information for the lettuce industry and associated breeding programs.

关键词： Lettuce Shelf life Nutrient retention Ascorbic acid Carotene Sugars

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