检索结果-南通市图书馆

Journal of Traditional Chinese Medicine 2024年第1期44卷 27-34页

作者： LI Xi LIN Xiangquan CHEN Dongdong LIU Hui the Third clinical Medical College Fujian Medical University department of Orthopedics Fuzhou Second HospitalFuzhou 350007China

OBJECTIVE:To explore the relationship between autophagy and apoptosis regulated by puerarin during ***:In this study,the effects of puerarin on the autophagic activity and apoptosis level of osteoblast precursors(MC3T3-E1 cells)was ***,the roles of puerarin on B-cell lymphoma-2(Bcl-2)phosphorylation at different sites in osteoblast precursors were *** effect of puerarin on the interaction between Bcl-2 and autophagy regulatory molecule or pro-apoptotic molecule was also investigated using Co-immunoprecipitation *** addition,the effect of puerarin on mitochondrial membrane potential of osteoblast precursors was also identified by mitochondrial membrane potential fluorescence probe ***:Our results showed that puerarin can promote the autophagic activity and apoptosis level of *** addition,puerarin promoted Bcl-2phosphorylation at Ser70 site,and the dissociation of Bcl-2-Beclin1 ***,puerarin could enhance the binding of Bcl-2-Bcl-2-Associated X(Bax)complex in MC3T3-E1 ***,puerarin increased the mitochondrial membrane potential of MC3T3-E1 ***:Therefore,puerarin promotes Beclin1into autophagy flux through Bcl-2 phosphorylation at Ser70,thereby enhancing autophagy of osteoblast precursors,which mediates its anti-apoptotic role during ***,the dissociation of Bcl-2-Beclin1 complex is conducive to the binding of Bcl-2-Bax complex,which resists the apoptosis of osteoblast precursors via the increased mitochondrial membrane potential.

关键词： puerarin osteoblasts autophagy apoptosis lymphoma B-cell

Investigation of the active ingredients and mechanism of Shuangling extract in dextran sulfate sodium salt induced ulcerative colitis mice based on network pharmacology and experimental verification

在线全文

学校读者我要写书评

暂无评论

Journal of Traditional Chinese Medicine 2024年第3期44卷 478-488页

作者： CHU Mengzhen WANG Yu LIN Zhijian Lyu Jintao ZHANG Xiaomeng ZHANG Bing department of clinical Chinese Pharmacy School of Chinese Materia MedicaBeijing University of Chinese MedicineBeijing 102488China

OBJECTIVE: To explore the pharmacodynamic effects and potential mechanisms of Shuangling extract against ulcerative colitis(UC). METHODS: The bioinformatics method was used to predict the active ingredients and action targets of Shuangling extract against UC in mice. And the biological experiments such as serum biochemical indexes and histopathological staining were used to verify the pharmacological effect and mechanism of Shuangling extract against UC in mice. RESULTS: The Shuangling extract reduced the levels of seruminterleukin-1β(IL-1β), tumor necrosis factor-α(TNF-N), interleukin-6(IL-6) and other inflammatory factors in UC mice and inhibited the inflammatory response. AKT Serine/threonine Kinase 1 and IL-6 may be the main targets of the anti-UC action of Shuangling extract, and the TNF signaling pathway, Forkhead box O signaling pathway and T-cell receptor signaling pathway may be the main signaling pathways. CONCLUSION: The Shuangling extract could inhibit the inflammatory response induced by UC and regulate intestinal immune function through multiple targets and multiple channels, which provided a new option and theoretical basis for anti-UC.

关键词： colitis ulcerative pharmacodynamic study network pharmacology molecular docking simulation Shuangling extract

Accurate prediction of biliary atresia with an integrated model using MMP-7 levels and bile acids

在线全文

学校读者我要写书评

暂无评论

World Journal of Pediatrics 2024年第8期20卷 822-833页

作者： Yi-Jiang Han Shu-Qi Hu Jin-Hang Zhu Xiao Cai Deng-Ming Lai Bao-Hai Chen Kun Zhu Qiao Tong Xin-Rui Zhou Jia-Le Deng Jin-Fa Tou Zhuo Fang Li-Zhong Du department of Neonatal Surgery Children’s HospitalZhejiang University School of MedicineNational Clinical Research Center for Child HealthHangzhouChina department of Data and Analytics WuXi Diagnostics Innovation Research InstituteShanghaiChina department of Information Center Children’s HospitalZhejiang University School of MedicineNational Clinical Research Center for Child HealthHangzhouChina department of Pathology Children’s HospitalZhejiang University School of MedicineNational Clinical Research Center for Child HealthHangzhouChina department of Neonatology Children’s HospitalZhejiang University School of MedicineNational Clinical Research Center for Child HealthHangzhouChina

Background Biliary atresia(BA)is a rare fatal liver disease in children,and the aim of this study was to develop a method to diagnose BA *** We determined serum levels of matrix metalloproteinase-7(MMP-7),the results of 13 liver tests,and the levels of 20 bile acids,and integrated computational models were constructed to diagnose *** Our findings demonstrated that MMP-7 expression levels,as well as the results of four liver tests and levels of ten bile acids,were significantly different between 86 BA and 59 non-BA patients(P<0.05).The computational prediction model revealed that MMP-7 levels alone had a higher predictive accuracy[area under the receiver operating characteristic curve(AUC)=0.966,95%confidence interval(CI):0.942,0.989]than liver test results and bile acid *** AUC was 0.890(95%CI 0.837,0.943)for liver test results and 0.825(95%CI 0.758,0.892)for bile acid ***,bile levels had a higher contribution to enhancing the predictive accuracy of MMP-7 levels(AUC=0.976,95%CI 0.953,1.000)than liver test *** AUC was 0.983(95%CI 0.962,1.000)for MMP-7 levels combined with liver test results and bile acid *** addition,we found that MMP-7 levels were highly correlated with gamma-glutamyl transferase levels and the liver fibrosis *** The innovative integrated models based on a large number of indicators provide a noninvasive and cost-effective approach for accurately diagnosing BA in children.

关键词： Bile aid.Biliary atresia Diagnosis Liver fibrosis Liver test MMP-7

Visceral adipose tissue predicts severity and prognosis of acute pancreatitis in obese patients

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Hepatobiliary & Pancreatic Diseases International 2024年第5期23卷 458-462页

作者： Yuan Tian Qing Huang Yu-Tang Ren Xuan Jiang Bo Jiang department of Gastroenterology Beijing Tsinghua Changgung HospitalSchool of Clinical MedicineTsinghua UniversityBeijing 102218China

Acute pancreatitis is a common systemic inflammatory disease, manifested by a spectrum of severity, ranging from mild in the majority of patients to severe acute pancreatitis. Patients with severe acute pancreatitis suffer from severe local and systemic complications and organ failure, leading to a poor prognosis. The early recognition of the severe condition is important to improve prognosis. Obesity has risen in tandem with an increase in the severity of acute pancreatitis in recent years. Studies have revealed that adipose tissue, particularly visceral adipose tissue is associated with the prognosis of acute pancreatitis. This review discussed the role of visceral adipose tissue in obese patients with acute pancreatitis and explored the possible mechanism involved.

关键词： Visceral adipose tissue Obesity Acute pancreatitis

Evaluation of the value of combined detection of tumor markers CA724,carcinoembryonic antigen,CA242,and CA19-9 in gastric cancer

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastrointestinal Oncology 2024年第5期16卷 1737-1744页

作者： Chong-Mei Zhou Shao-Hua Zhao department of clinical Laboratory Henan Provincial People's HospitalZhengzhou 450003Henan ProvinceChina

BACKGROUND Gastric cancer is a global health concern that poses a significant threat to human *** To detecting serum changes in carcinoembryonic antigen(CEA),carbohydrate antigens(CA)724,CA242,and CA19-9 expression among patients with gastric *** Eighty patients diagnosed with gastric cancer between January 2020 and January 2023 were included in the observation group,while 80 patients with benign gastric diseases were included in the control *** groups were tested for tumor markers(CA724,CEA,CA242,and CA19-9].Tumor marker indicators(CA724,CEA,CA242,and CA19-9)were compared between the two groups,assessing positive rates of tumor markers across various stages in the observation ***,single and combined detection of various tumor markers were *** The sensitivity,specificity,accuracy,positive predictive value,and negative predictive value observed for the combined detection of CA724,CEA,CA242,and CA19-9 were higher than those of CA724,CEA,CA242,and CA19-9 ***,the combined detection of CA724,CEA,CA242,and CA19-9 has a high diagnostic accuracy and could reduce the occurrence of missed or misdiagnosed cases,facilitating the early diagnosis and treatment of *** CA724,CEA,CA242,and CA19-9 serum levels in gastric cancer patients significantly surpassed those in non-gastric cancer patients(Pclinical promotion.

关键词： Biomarkers Serum Gastric cancer Diagnostic value

DNA methylation drives a new path in gastric cancer early detection:Current impact and prospects

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genes & Diseases 2024年第2期11卷 847-860页

作者： Xinhui Wang Yaqi Dong Hong Zhang Yinghui Zhao Tianshu Miao Ghazal Mohseni Lutao Du Chuanxin Wang department of clinical Laboratory The Second Hospital of Shandong UniversityJinanShandong 250033China department of clinical Laboratory Fuling HospitalChongqing UniversityChongqing 402774China Suzhou Research Institute of Shandong University SuzhouJiangsu 215123China department of Biochemistry and Molecular Biology Shandong University School of Basic Medical SciencesJinanShandong 250012China Shandong Engineering & Technology Research Center for Tumor Marker Detection JinanShandong 250033China Shandong Provincial clinical Medicine Research Center for clinical Laboratory JinanShandong 250033China

Gastric cancer(GC)is one of the most common and deadly cancers *** detection offers the best chance for curative treatment and reducing its ***,the optimal population-based early screening for GC remains *** DNA methylation occurs in the early stage of GC,exhibiting cancer-specific genetic and epigenetic changes,and can be detected in the media such as blood,gastric juice,and feces,constituting a valuable biomarker for cancer early ***,DNA methylation is a stable epigenetic alteration,and many innovative methods have been developed to quantify it rapidly and ***,large-scale clinical validation of DNA methylation serving as tumor biomarkers is still lacking,precluding their implementation in clinical *** conclusion,after a critical analysis of the recent existing literature,we summarized the evolving roles of DNA methylation during GC occurrence,expounded the newly discovered noninvasive DNA methylation biomarkers for early detection of GC,and discussed its challenges and prospects in clinical applications.

关键词： Biomarkers DNA methylation Early detection Gastric cancer Translation

Accuracy evaluation of Roche Accu-Chek Performa blood glucose meters at low glucose concentrations:A nine-year retrospective study

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

iLABMED 2024年第3期2卷 141-148页

作者： Zhipeng Zhao Runqing Li Xiuying Zhao Lina Zhang Tengjiao Wang Song Yang Ning Han Dong Zhu Laboratory Medicine department of Beijing Tsinghua Changgung Hospital School of Clinical MedicineTsinghua UniversityBeijingChina operations Management department of Beijing Tsinghua Changgung Hospital School of Clinical MedicineTsinghua UniversityBeijingChina

Objective:To evaluate the accuracy of Roche Accu-Chek Performa glucose meters at a low glucose concentration of<5.55 mmol/L(100 mg/dL)over a 9-year ***:The accuracy of the Roche Accu-Chek Performa glucose meters at lo... 详细信息

Objective:To evaluate the accuracy of Roche Accu-Chek Performa glucose meters at a low glucose concentration ofclinical use duration spanned from 1 to 9 years and 40.76%(86 out of 211 glucose meters)had been used for more than 2 *** correlation coefficient r between glucose meter measurements and laboratory reference values was 0.98(p<0.001).The mean according to Roche Accu-Chek Performa glucose meters was 0.05 mmol/L(0.9 mg/dL)higher than that of the biochemical analyzer(Z=-13.82,p<0.0001).The results showed that 100.00%(211 out of 211)of the Roche Accu-Chek Performa glucose meters met the acceptability criteria specified in ISO 15197:*** a low glucose concentration of<5.55 mmol/L,99.90%(2975 out of 2978)of the comparative data pairs in the error distribution fell within the range of±0.83 mmol/L(15 mg/dL).Parkes consensus error grid analysis showed that 100.00%(2978 out of 2978)of comparative data pairs fell within region ***:This study demonstrated that Roche Accu-Chek Performa glucose meters successfully met the accuracy standards of ISO 15197:2013 for measuring blood glucose within the hypoglycemic *** attention should be given to the performance of blood glucose monitoring systems in the

关键词： blood glucose monitoring system comparative study diabetes low glucose concentration point-of-care

NLRP3 activation in macrophages promotes acute intestinal injury in neonatal necrotizing enterocolitis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Pediatrics 2024年第2期20卷 153-164页

作者： Bo Shi Cheng-Jie Lyu Zhen-Kai Le Hao-Sen Ji Yi Xiao Yuan-Yuan Zhang Shou-Jiang Huang Lin-Jun Yu Qiang Shu Jin-Fa Tou Deng-Ming Lai department of Neonatal Surgery Children’s HospitalZhejiang University School of MedicineNational Clinical Research Center for Child HealthHangzhou 310052China department of Pulmonology Children’s HospitalZhejiang University School of MedicineNational Clinical Research Center for Child HealthHangzhou 310052China department of Thoracic and Cardiovascular Surgery Children’s HospitalZhejiang University School of MedicineNational Clinical Research Center for Child HealthHangzhou 310052China

Background Macrophages are involved in various immune inflammatory disease *** study aimed to investigate the role and mechanism of macrophages in regulating acute intestinal injury in neonatal necrotizing enterocolitis(NEC).Methods CD68,nucleotide-binding oligomerization domain,leucine-rich repeat,and pyrin domain-containing 3(NLRP3),cysteine aspartate-specific protease-1(caspase-1),and interleukin-1β(IL-1β)in paraffin sections of intestinal tissues from NEC and control patients were detected with immunohistochemistry,immunofluorescence,and western *** pet milk,hypoxia and cold stimulation were used to establish a mouse(wild type and Nlrp3^(-/-))model of *** mouse macrophage(RAW 264.7)and rat intestinal epithelial cell-6 lines were also cultured followed by various ***,intestinal epithelial cell injuries,and IL-1β release were *** Compared to the gut“healthy”patients,the intestinal lamina propria of NEC patients had high macrophage infiltration and high NLRP3,caspase-1,and IL-1β ***,in vivo,the survival rate of Nlrp3^(-/-)NEC mice was dramatically improved,the proportion of intestinal macrophages was reduced,and intestinal injury was decreased compared to those of wild-type NEC ***3,caspase-1,and IL-1β derived from macrophages or supernatant from cocultures of macrophages and intestinal epithelial cells also caused intestinal epithelial cell *** Macrophage activation may be essential for NEC ***3/caspase-1/IL-1β cellular signals derived from macrophages may be the underlying mechanism of NEC development,and all these may be therapeutic targets for developing treatments for NEC.

关键词： Caspase-1 Interleukin-1β Macrophage Necrotizing enterocolitis NLRP3

Progress on immuno-microenvironment and immune-related therapies in patients with pseudomyxoma peritonei

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cancer Biology & Medicine 2024年第7期21卷 586-605页

作者： Qidi Zhao Tian Wei Ru Ma Yubin Fu Rui Yang Yandong Su Yang Yu Bing Li Yan Li School of clinical Medicine Tsinghua UniversityBeijing 100084China department of Surgical Oncology Beijing Tsinghua Changgung HospitalSchool of Clinical MedicineTsinghua UniversityBeijing 102218China department of Peritoneal Cancer Surgery Bejing Shijitan HospitalCapital Medical UniversityBejing 100038China

Pseudomyxoma peritonei(PMP) is an indolent malignant syndrome. The standard treatment for PMP is cytoreductive surgery combined with intraperitoneal hyperthermic chemotherapy(CRS + HIPEC). However, the high recurrence rate and latent clinical symptoms and signs are major obstacles to further improving clinical outcomes. Moreover, patients in advanced stages receive little benefit from CRS + HIPEC due to widespread intraperitoneal metastases. Another challenge in PMP treatment involves the progressive sclerosis of PMP cell-secreted mucus, which is often increased due to activating mutations in the gene coding for guanine nucleotide-binding protein alpha subunit(GNAS). Consequently, the development of other PMP therapies is urgently needed. Several immune-related therapies have shown promise, including the use of bacterium-derived non-specific immunogenic agents, radioimmunotherapeutic agents, and tumor cell-derived neoantigens, but a well-recognized immunotherapy has not been established. In this review the roles of GNAS mutations in the promotion of mucin secretion and disease development are discussed. In addition, the immunologic features of the PMP microenvironment and immune-associated treatments are discussed to summarize the current understanding of key features of the disease and to facilitate the development of immunotherapies.

关键词： Pseudomyxoma peritonei tumor immuno-microenvironment immune-related therapy mucin 2 GNAS mutation

Characterization of the CpsQ Regulon Reveals Its Role in the Transcription of Type Ⅵ Secretion System 2 Genes in Vibrio parahaemolyticus

在线全文

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2024年第4期37卷 436-439页

作者： LUO Xi LI Xue ZHANG Miao Miao HUANG Qing Lian ZHANG Yi Quan JI Shen Jie LU Ren Fei department of clinical Laboratory Affiliated Nantong Hospital 3 of Nantong UniversityNantong Third People’s HospitalNantong 226006JiangsuChina department of clinical Laboratory Qidong People’s HospitalQidong 226200JiangsuChina

Vibrio parahaemolyticus,a gram-negative halophilic bacterium that naturally inhabits coastal waters,causes gastroenteritis,skin infections,and septicemia in human beings[1].This bacterium produces multiple virulence factors,including thermostable direct hemolysin(TDH),TDH-related hemolysin(TRH),type Ⅲ secretion system 1(T3SS1),T3SS2,type Ⅵ secretion system 1(T6SS1),and T6SS2[1].Furthermore,Ⅴ.parahaemolyticus forms biofilms on the surface,which help it in adapting to unfavorable conditions[2].Mature biofilm formation requires special structures,including lateral and polar flagella,exopolysaccharide(EPS),and type Ⅳ pili[2].

关键词： coastal system T3SS