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Durable CRISPR-Based Epigenetic Silencing

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BioDesign Research 2021年第1期2021卷 39-46页

作者： Muneaki Nakamura Alexis E.Ivec Yuchen Gao Lei S.Qi department of Bioengineering StanfordCA 94305USA Program in Human biology StanfordCA 94305USA Cancer biology Program StanfordCA 94305USA department of chemical and Systems biology StanfordCA 94305USA chem-H Institute StanfordCA 94305USA

Development of CRISPR-based epigenome editing tools is important for the study and engineering of biological ***,we describe the design of a reporter system for quantifying the ability of CRISPR epigenome editors to produce a stable gene *** characterize the dynamics of durable gene silencing and reactivation,as well as the induced epigenetic changes of this *** report the creation of single-protein CRISPR constructs bearing combinations of three epigenetic editing domains,termed KAL,that can stably repress the gene *** system should allow for the development of novel epigenome editing tools which will be useful in a wide array of biological research and engineering applications.

关键词： expression behavior CRISPR

Theoretical Study on the Electronic Structures of Small Ti_nN_m(n+m=5,6)Clusters

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Chinese Journal of Structural chemistry 2008年第10期27卷 1281-1287页

作者：朱纯谭凯林梦海张乾二 department of chemistry Xiamen University State Key Laboratory of Phys.chem.of Solid Surfaces department of chem.Science College Guizhou University

45 isomers of TinNm （n ＋ m = 5, 6） clusters, including linear, some planar and some stero configurations, have been predicted by density functional theory method. For five-atom clusters Ti3N2 and Ti2N3, the most stable structures are trigonal bipyramid in D3h symmetry, and for TiaN cluster, the isomer with one nitrogen atom occupying the center of quasi-tetrahedron is the most stable. In the isomers of Ti4N2 and Ti3N3, the planar networks are more stable, but for Ti2N4, the six-membered ring configuration is the most favorable. Most linear structures can form weak-strong bonds alternately with higher energy. As regards to planar structures, the more Ti-N bonds are formed, the more stable they will be; for stero closed polyhedral isomers, their energies are lower.

关键词： TinNm cluster quantum chemical study

Studies on chem icalCom position ofIron-m olybdenum Cofactor fromNitrogenase by Fluorescence Analysis

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chemical Research in Chinese Universities 1999年第3期15卷 286-293页

作者： ZHANG Feng-zhang XU Liang-shu HUANG He-qing LONG Min-nan QIU Xue-hui CHEN Zhong department of biology Xiamen UniversityXiamen 361005P.R.China

The iron molybdenum cofactor(FeMoco) of nitrogenase MoFe protein from Azotobacter vinelandii OP was extracted by N methylformamide(NMF). The effects of FeMoco(in NMF) on electronic spectrum and fluorescence intensity of fluorescein dimercury acetate(FDMA)(in 1 mol/L NaOH) were investigated by fluorophotometric titrations and compared with those of (NH 4) 2MoS 4 and complexes of (NH 4) 2MoS 4 with Na 2S or Na 2S 2 or (NH 4) 2S x on the relative properties of FDMA. It was found that the electronic spectrum of FDMA displayed hypsochromic shift(17 nm) in the presence of FeMoco just like that in the presence of other inorganic sulfides and that the titration curve for the quench of FDMA with FeMoco is very similar to that for the quench of FDMA with complex of (NH 4) 2MoS 4 with Na 2S 2(mole ratio is 1∶3). The results showed that FeMoco(N) probably contained S—S bonds and its structure was found to be changed compared with Kim Rees structural model. This change should profit the formation of the polymer.

关键词： Nitrogenase Iron molybdenum cofactor Fluorophotometric titration Structural change

Biological functions and diagnostic implications of microRNAs in Mycobacterium tuberculosis infection

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Asian Pacific Journal of Tropical Biomedicine 2022年第1期12卷 1-8页

作者： Godkowicz Magdalena Druszczynska Magdalena department of Immunology and Infectious biology Institute of MicrobiologyBiotechnology and ImmunologyFaculty of Biology and Environmental ProtectionUniversity of LodzBanacha 12/1690-237 LodzPoland The Bio-Med-chem Doctoral School of the University of Lodz and Lodz Institutes of the Polish Academy of Sciences LodzPoland

MicroRNAs(miRNAs),small non-coding RNAs,play important roles in regulating host defense against pathogenic *** review provides information on the role of miRNAs in the antimycobacterial immune response and summarizes their possible diagnostic *** was compiled using scientific literature retrieved from such databases as PubMed,Scopus,ScienceDirect,Google Scholar,and PubMed *** articles published in the English language until December 2020 were taken into *** has been revealed that specific host miRNAs induced by Mycobacterium tuberculosis can target diverse factors and pathways in immune signaling to ensure longer pathogen survival inside the *** potential use of miRNAs in tuberculosis diagnosis or therapeutic strategies has been attracting increasing attention in recent ***,despite considerable efforts devoted to miRNA profiling,further studies are needed to elucidate the full potential of miRNAs as novel tuberculosis biomarkers or therapeutic targets.

关键词： microRNA Mycobacterium tuberculosis Biomarker Diagnostics Tuberculosis

A new rhodamine-based fluorescent chemosensor for mercury in aqueous media

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Chinese chemical Letters 2014年第7期25卷 1065-1068页

作者： Yao Liu Er-Bing Yang Rui Han Di Zhang Yong Ye Yu-Fen Zhao Phosphorus chemical Engineering Research Center of Henan Province the College of Chemistry and Molecular EngineeringZhengzhou University Key Laboratory of Bioorganic Phosphorus chemistry & chemical biology (Ministry of Education) Department of ChemistryTsinghua University Key Lab chem Biol Fujian Prov. Coll. Chem.& Chem. Engn.Xiamen University

A new fluorescent ＂on-off＇ chemosensor for Hg2＋ initiated by a derivative of rhodamine B was designed and synthesized. Compound 1 exhibited high sensitivity and selectivity for Hg2＋ over other commonly coexistent metal ions in aqueous media, Upon the addition of Hg2＋, the spirocyclic ring of probe is opened and a significant enhancement of visible color and fluorescence in the range of 500-600 nm is observed, The colorimetric and fluorescent response to Hg2＋ can be conveniently detected by the naked eye, which provides a facile method for visual detection of Hg2＋, From the molecular structure and spectral results of 1, an irreversible, hydrolysis, desulfurization reaction mechanism is proposed.

关键词： Fluorescent probe Rhodamine Mercury On-off chemosensor

ENZYMATICALLY　SOLVENT－FREE　SYNTHESIS　OF　SUGAR　ESTER　CONTAINING　ω－3　POLYUNSATURATED　FATTY　ACID

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Chinese chemical Letters 1996年第7期7卷 611-614页

作者： Zu Yi Li Owen P. Ward(1.Shanghai Institute of Organic chemistry Academia Sinica Shanghai,200032)(2.department of biology,University of Waterloo,Waterloo,Ontario N2L3GI,Canada) Shanghai Inst. Organ. chem. Acad. S. Shanghai 200032 department of biology University of Waterloo Waterloo Ont. N2L3GI Canada

A enzymatic synthesis of sugar ester from isopropylidenesugar and ω-3polyunsaturated fatty acid was *** with Lipase N-435 gave the bestresult.

关键词： ACID FATTY ENZYMATICALLY CONTAINING

Targeted protein degradation bypassing cereblon and von Hippel-Lindau

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The Innovation 2023年第3期4卷 42-43页

作者： Wenchao Lu Lingang Laboratory Shanghai 200031China department of chemical and Systems biology Chem-H and Stanford Cancer InstituteStanford School of MedicineStanford UniversityStanfordCA 94305USA

Targeted protein degradation(TPD)has emerged as a new drug discovery approach to wipe out disease-causing proteins by harnessing the ubiquitin-proteasome system(UPS).A major class of molecules in the field are called proteolysis-targeting chimeras(PROTACs),which are heterobifunctional molecules encompassing two ligands that bind to a protein of interest and the E3 ligase,respectively joint by a linker.1 Due to its catalytic mechanism that enables event-driven pharmacology,PROTACs technology can induce an efficient degradation and therefore achieve the pharmacology at a lower dose compared with traditional small molecule inhibitors that exert their inhibition through an occupancy-driven *** the first PROTAC was reported as a proof-ofconcept 20 years ago,this technology has led to a paradigm shift that culminated in the clinic trials of new modalities such as ARV-110,ARV-471,etc.

关键词： mechanism proof enable

Progress and challenges for replacing n-methyl-2-pyrrolidone/polyvinylidene fluoride slurry formulations in lithium-ion battery cathodes

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Progress in Natural Science:Materials International 2024年第1期34卷 194-206页

作者： Sung Cik Mun Yeong Hoon Jeon Jong Ho Won Battery Materials Research Center LG Chem department of Energy Engineering Dankook University

With electric vehicles, energy storage systems, and portable electronic devices becoming increasingly popular, the demand for lithium-ion batteries has surged considerably. In the lithium-ion battery industry, n-methyl-2-pyrrolidone(NMP) is widely used as the solvent for cathode slurry, and polyvinylidene fluoride(PVDF) is used as the cathode binder. However, because of the harmful effect of NMP on the environment and human health, the use of NMP and PVDF for lithium-ion batteries will be highly regulated in the future. Therefore, developing eco-friendly alternatives is crucial for formulating systems using new solvents and binders. Despite numerous efforts, these alternatives have not been widely adopted across the lithium-ion battery industry. It is due to their limited ability to compete on price or performance to replace the traditional NMP/PVDF slurry formulations. This review investigates developments in the search for new solvents and binders that can be used in cathode slurry compositions. The new systems can potentially decrease energy consumption and manufacturing costs associated with NMP recovery, energy-intensive drying processes, and material expenses. We discuss key factors and technical challenges from earlier studies and compare them with the current, optimized formulations based on NMP and PVDF.

关键词： Lithium-ion battery Eco-friendly alternative Slurry formulation chemical regulation Binder performance

The use of computational thermodynamic for yttrium recovery from rare earth elements-bearing residue

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Journal of Rare Earths 2021年第2期39卷 201-207页

作者： Amilton Barbosa Botelho Junior Denise Crocce Romano Espinosa Jorge Alberto Soares Tenorio department of chem ical Engineering Polytechnic SchoolUniversity of Sao PauloSao PauloBrazil

The rare earth elements are considered critical metals,due to the risk of supply *** recycling of waste electrical and electronic equipment can be an alternative to supply the rare earth *** processes have been developed,and by aqueous media is the most prominent,which makes possible the extraction and separation of elements even in low concentration(traces).As an example of thermal processing,the use of thermodynamic simulations might benefit the metal extraction in hydrometallurgical *** this reason,the goal of this work is to evaluate the use of FactSage 7.2 software for the leaching of fluorescent lamp powders by sulfuric *** effect of concentration and temperature was *** comprise that the thermodynamic software wellpredicted the solid phase formed in all residues of leaching experiments-gypsum was predicted by the software and identified in XRD *** demonstrates that FactSage software can be explored for metals extraction in aqueous media,being important for trace-elements *** extraction reaches up to 95%at 45℃using H_(2)SO_(4)2 mol/L.

关键词： Yttrium Lanthanum Cerium WEEE FactSage Rare earths