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Identifying genetic susceptibility to Aspergillus fumigatus infection using collaborative cross mice and RNA-Seq approach

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Animal Models and Experimental Medicine 2024年第1期7卷 36-47页

作者： Roa'a H.S.Yosief Iqbal M.Lone Aharon Nachshon Heinz Himmelbauer Irit Gat-Viks Fuad A.Iraqi department of Clinical microbiology and Immunology Sackler Faculty of MedicineTel-Aviv UniversityTel-AvivIsrael School of Molecular Cell Biology and biotechnology George S.Wise Faculty of Life SciencesTel Aviv UniversityTel AvivIsrael Institute of Computational Biology Department of BiotechnologyUniversity of Natural Resources and Life SciencesMuthgasse 181190 ViennaAustria

Background:Aspergillus fumigatus(Af)is one of the most ubiquitous fungi and its infection potency is suggested to be strongly controlled by the host genetic *** aim of this study was to search for candidate genes associated with host susceptibility to Aspergillus fumigatus(Af)using an RNAseq approach in CC lines and hepatic gene ***:We studied 31 male mice from 25 CC lines at 8 weeks old;the mice were infected with *** tissues were extracted from these mice 5 days post-infection,and next-generation RNA-sequencing(RNAseq)was *** GENE-E analysis platform was used to generate a clustered heat map ***:Significant variation in body weight changes between CC lines was *** gene expression revealed 12 top prioritized candidate genes differ-entially expressed in resistant versus susceptible mice based on body weight ***,three candidate genes are located within genomic intervals of the previ-ously mapped quantitative trait loci(QTL),including Gm16270 and Stox1 on chromo-some 10 and Gm11033 on chromosome ***:Our findings emphasize the CC mouse model's power in fine mapping the genetic components underlying susceptibility towards *** a next step,eQTL analysis will be performed for our RNA-Seq *** candidate genes from our study will be further assessed with a human cohort with aspergillosis.

关键词： aspergillus fumigatus infection collaborative cross(CC)mice gene expression profile gene-network host susceptibility quantitative trait loci(QTL)mapping RNA-Seq

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Alterations in the gut microbiota and the faecal metabolomes are potentially associated with gestational diabetes mellitus through inflammatory response

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food Science and Human Wellness 2024年

作者： Ruili Pan Xiaopeng Xu Ying Chen Haojue Wang Linlin Wang Peijun Tian Xing Jin Jianxin Zhao Wei Chen Gang Wang State Key Laboratory of food Science and Resources， Jiangnan University School of food Science and Technology， Jiangnan University The department of Clinical Laboratory， Wuxi Xishan People's Hospital The department of Obstetrics and Gynecology， Wuxi Xishan People's Hospital National Engineering Research Center for Functional food， Jiangnan University (Yangzhou) Institute of food biotechnology， Jiangnan University

Gestational diabetes mellitus (GDM) is a disease of glucose intolerance that first occurs during pregnancy. Accumulating evidence underlined a link between gut microbiota dysbiosis and GDM， and microbial metabolites represent a unique way to explore microbiota–host interactions. However， the associations between changes in the gut microbiota and microbial metabolites and immune homeostasis in the GDM pathogenesis remain largely unclear. In this prospective study， the characteristics of gut microbiota in both first trimester (T1) and second trimester (T2 ) were investigated in 46 GDM patients and 44 matched controls. We comprehensively profiled the microbial metabolites using non-targeted metabolomics and quantitatively targeted metabolomics， measurements of inflammatory cytokines and biomarkers of intestinal barrier function， and combined with correlation analysis in T2. Gut microbiota dybiosis was observed in GDM patients in both T1 and T2， and was characterised by the enrichment of multiple potentially harmful bacteria， such as UBA1819 and Erysipelatoclostridium. Besides， alterations in the microbiota were accompanied by a disturbance in tryptophan metabolism， mainly manifested as a shift towards the production of more kynurenine and less indole derivatives. Most importantly， correlation network analysis indicated that overgrowth of potential pathogens and tryptophan metabolism disorder were associated with inflammatory imbalance and disrupted epithelial barrier in GDM patients. Theses findings provide a greater understanding of the pathogenesis and new targets for microecological interventions by mediating tryptophan metabolism in GDM.

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Gut liver brain axis in diseases:the implications for therapeutic interventions

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Signal Transduction and Targeted Therapy 2024年第1期9卷 79-104页

作者： Mengyao Yan Shuli Man Benyue Sun Long Ma Lanping Guo Luqi Huang Wenyuan Gao State Key Laboratory of food Nutrition and Safety Key Laboratory of Industrial MicrobiologyMinistry of EducationTianjin Key Laboratory of Industry MicrobiologyNational and Local United Engineering Lab of Metabolic Control Fermentation TechnologyChina International Science and Technology Cooperation Base of Food Nutrition/Safety and Medicinal ChemistryCollege of BiotechnologyTianjin University of Science&Technology300457 TianjinChina National Resource Center for Chinese Materia Medica China Academy of Chinese Medical Sciences100700 BeijingChina Tianjin Key Laboratory for Modern Drug Delivery&High-Efficiency School of Pharmaceutical Science and TechnologyTianjin UniversityWeijin Road300072 TianjinChina

Gut-liver-brain axis is a three-way highway of information interaction system among the gastrointestinal tract,liver,and nervous *** the past few decades,breakthrough progress has been made in the gut liver brain axis,mainly through understanding its formation mechanism and increasing treatment *** this review,we discuss various complex networks including barrier permeability,gut hormones,gut microbial metabolites,vagus nerve,neurotransmitters,immunity,brain toxic metabolites,β-amyloid(Aβ)metabolism,and epigenetic regulation in the gut-liver-brain *** therapies containing antibiotics,probiotics,prebiotics,synbiotics,fecal microbiota transplantation(FMT),polyphenols,low FODMAP diet and nanotechnology application regulate the gut liver brain ***,some special treatments targeting gut-liver axis include farnesoid X receptor(FXR)agonists,takeda G protein-coupled receptor 5(TGR5)agonists,glucagon-like peptide-1(GLP-1)receptor antagonists and fibroblast growth factor 19(FGF19)*** gut-brain axis embraces cognitive behavioral therapy(CBT),antidepressants and tryptophan metabolism-related *** liver-brain axis contains epigenetic regulation and Aβmetabolism-related *** the future,a better understanding of gut-liver-brain axis interactions will promote the development of novel preventative strategies and the discovery of precise therapeutic targets in multiple diseases.

关键词： metabolism therapeutic immunity

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Two intestinal microbiota-derived metabolites, deoxycholic acid and butyrate, synergize to enhance host defense peptide synthesis and alleviate necrotic enteritis

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Journal of Animal Science and biotechnology 2024年第3期15卷 1158-1172页

作者： Dohyung M.Kim Jing Liu Melanie A.Whitmore Isabel Tobin Zijun Zhao Guolong Zhang department of Animal and food Sciences Oklahoma State UniversityStillwaterOKUSA

Background Necrotic enteritis(NE)is a major enteric disease in poultry,yet effective mitigation strategies remain *** acid(DCA)and butyrate,two major metabolites derived from the intestinal microbiota,have independently been shown to induce host defense peptide(HDP)***,the potential synergy between these two compounds remains *** To investigate the possible synergistic effect between DCA and butyrate in regulating HDP synthesis and barrier function,we treated chicken HD11 macrophage cells and jejunal explants with DCA and sodium butyrate(NaB),either individually or in combination,for 24 ***,we performed RNA isolation and reverse transcrip-tion-quantitative PCR to analyze HDP genes as well as the major genes associated with barrier *** further determine the synergy between DCA and NaB in enhancing NE resistance,we conducted two independent trials with Cobb broiler *** each trial,the diet was supplemented with DCA or NaB on the day-of-hatch,followed by NE induction through sequential challenges with Eimeria maxima and Clostridium perfringens on d 10 and 14,*** recorded animal mortality after infection and assessed intestinal lesions on d *** impact of DCA and NaB on the microbiota in the ileum and cecum was evaluated through bacterial 16S rRNA gene *** We found that the combination of DCA and NaB synergistically induced multiple HDP genes in both chicken HD11 cells and jejunal ***,the gene for claudin-1,a major tight junction protein,also exhibited synergistic induction in response to DCA and ***,dietary supplementation with a combination of 0.75 g/kg DCA and 1 g/kg NaB led to a significant improvement in animal survival and a reduction in intestinal lesions compared to either compound alone in a chicken model of ***,the cecal microbiota of NE-infected chickens showed a marked decrease in SCFA-producing bacteria such as Bacteroides,F

关键词： Bile acids Host defense peptides Metabolites Microbiome Necrotic enteritis Short-chain fatty acids

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High glucose reduces Nrf2-dependent cRAGE release and enhances inflammasome-dependent IL-1βproduction in monocytes:the modulatory effects of EGCG

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food Science and Human Wellness 2024年第3期13卷 1531-1542页

作者： Chi-Hao Wu Yin-Hsuan Chang Chin-Lin Hsu Sheng-Yi Chen Gow-Chin Yen Graduate Programs of Nutrition Science School of Life ScienceTaiwan Normal UniversityTaipei 106209TaiwanChina department of food Science and biotechnology Chung Hsing UniversityTaichung 40227TaiwanChina School of Nutrition Chung Shan Medical UniversityTaichung 40201TaiwanChina

Soluble receptor for advanced glycation end products(sRAGE)acts as a decoy sequestering of RAGE ligands,thus preventing the activation of the ligand-RAGE axis linking human ***,the molecular mechanisms underlying sRAGE remain *** this study,THP-1 monocytes were cultured in normal glucose(NG,5.5 mmol/L)and high glucose(HG,15 mmol/L)to investigate the effects of diabetesrelevant glucose concentrations on sRAGE and interleukin-1β(IL-1β)*** modulatory effects of epigallocatechin gallate(EGCG)in response to HG challenge were also *** enhanced intracellular reactive oxygen species(ROS)generation and RAGE *** secretion of sRAGE,including esRAGE and cRAGE,was reduced under HG conditions,together with the downregulation of a disintegrin and metallopeptidase 10(ADAM10)and nuclear factor erythroid 2-related factor 2(Nrf2)nuclear ***,the HG effects were counteracted by siRAGE and exacerbated by *** immunoprecipitation results showed that Nrf2 binding to the ADAM10 promoter and HG interfered with this *** data reinforce the notion that RAGE and Nrf2 might be sRAGE-regulating *** HG conditions,the treatment of EGCG reduced ROS generation and RAGE ***-stimulated cRAGE release was likely caused by the upregulation of the Nrf2-ADAM10 *** inhibited HG-mediated NLRP3 inflammasome activation at least partly by stimulating sRAGE,thereby reducing IL-1βrelease.

关键词： Epigallocatechin gallate(EGCG) Inflammasome Nuclear factor erythroid 2-related factor 2(Nrf2) Receptor for advanced glycation end products(RAGE) Soluble RAGE(sRAGE)

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Combined Application of Biostimulants and EDTA Improved Wheat Productivity under Cadmium Stress

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Phyton-International Journal of Experimental Botany 2024年第7期93卷 1647-1665页

作者： Abida Aziz Shafiqa Bano Mubshar Hussain Muhammad Farooq Azhar Ghulam Yasin Naila Hadayat Iqra Arooj Abeer Hashem Ajay Kumar Elsayed Fathi Abd_Allah Qamar uz Zaman department of Botany The Women UniversityMultan54500Pakistan Institute of Agronomy Bahauddin Zakariya UniversityMultan60800Pakistan department of Forestry and Range Management Bahauddin Zakariya UniversityMultan60800Pakistan department of Botany Division of Science&TechnologyUniversity of EducationLahore54770Pakistan department of microbiology&Molecular Genetics The Women UniversityMultan54500Pakistan Botany and microbiology department College of ScienceKing Saud UniversityRiyadh201313Saudi Arabia Amity Institute of biotechnology Amity UniversityNoida201313India Plant Production department College of Food and Agricultural SciencesKing Saud UniversityRiyadh11451Saudi Arabia School of Agriculture Yunnan UniversityKunmingYunan650504China department of Environmental Sciences The University of LahoreLahore54590Pakistan

Wheat(Triticum aestivum L.)exhibits a greater capacity for cadmium(Cd)absorption compared to other cereal crops,leading to elevated daily Cd intake,and posing a signiﬁcant threat to public *** the mitigation of Cd stress in sustainable and environmentally friendly way,a pot study was designed by using exogenous application of various biostimulants,i.e.,Nigella sativa and Ocimum sanctum extracts:0%,10%,and 20%in combination with the chelating agent ethylenediaminetetraacetic acid(EDTA)using 0 and 5 mg kg^(-1) under various levels of Cd stress(i.e.,0,5,10,and 15 mg kg^(-1) soil).Results revealed that Cd stress signiﬁcantly reduced the seed emergence,growth,root,and allometric characters and yield-related parameters of wheat *** most observable reduc-tion was documented in wheat plants exposed to a higher Cd concentration(15 mg kg^(-1)),followed by the lower Cd level(control).The combined application of bio-stimulants and EDTA minimized the negative impacts of Cd *** highest increase in seedling emergence(5.44%),leaf area(50.60%),number of tillers(31.02%),grain yield per plant(24.28%),biological yield(13.97%),and decrease in Cd levels in grains(40%)was noticed where 20%foliar application of *** and 10%of *** biostimulants were done using 5 mg kg^(-1) of soil-applied *** intervention demonstrated a notable reduction in Cd-induced negative effects,highlighting the potential of these substances in promoting sustainable wheat cultivation in contaminated ***,it is an eco-friendly and approachable method at theﬁeld level able to ensure food safety.

关键词： Abiotic stress allometric characters antioxidant activity crop yield seed emergence grain yield

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Lactiplantibacillus plantarum AR113 alleviates microbiota dysbiosis of tongue coating and cerebral ischemia/reperfusion injury in rat

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food Science and Human Wellness 2024年第4期13卷 2132-2140页

作者： Zhiqiang Xiong Gang Liu Ling Fang Xiuming Li Yongjun Xia Guangqiang Wang Xin Song Lianzhong Ai Shanghai Engineering Research Center of food microbiology School of Health Science and EngineeringUniversity of Shanghai for Science and TechnologyShanghai 200093China department of Rehabilitation Huashan HospitalFudan UniversityShanghai 200040China department of Rehabilitation Medicine Ruijin HospitalShanghai Jiao Tong University School of MedicineShanghai 200025China

Stroke is one of the leading causes of death and disability ***,information on stroke-related tongue coating microbiome(TCM)is limited,and whether TCM modulation could benefit for stroke prevention and rehabilitation is ***,TCM from stroke patients(SP)was characterized using molecular *** occurrence of stroke resulted in TCM dysbiosis with significantly reduced species richness and *** abundance of Prevotella,Leptotrichia,Actinomyces,Alloprevotella,Haemophilus,and TM7_[G-1]were greatly reduced,but common infection Streptococcus and Pseudomonas were remarkably ***,an antioxidative probiotic Lactiplantibacillus plantarum AR113 was used for TCM intervention in stroke rats with cerebral ischemia/reperfusion(I/R).AR113 partly restored I/R induced change of TCM and gut microbiota with significantly improved neurological deficit,relieved histopathologic change,increased activities of antioxidant enzymes,and decreased contents of oxidative stress ***,the gene expression of antioxidant-related proteins and apoptosis-related factors heme oxygenase-1(HO-1),superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),nuclear factor erythroid 2-related factor 2(Nrf2),NAD(P)H:quinone oxidoreductase-1(NQO-1),and Bcl-2 was significantly increased,but cytochrome C,cleaved caspase-3,and Bax were markedly decreased in the brain by AR113 *** results suggested that AR113 could ameliorate cerebral I/R injury through antioxidation and anti-apoptosis pathways,and AR113 intervention of TCM may have the application potential for stroke prevention and control.

关键词： Stroke Cerebral ischemia/reperfusion Tongue coating Lactiplantibacillus plantarum AR113 Probiotic intervention

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Asymmetric reduction of conjugated C=C bonds by immobilized fusion of old yellow enzyme and glucose dehydrogenase

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Green Synthesis and Catalysis 2024年第2期5卷 80-87页

作者： Yongxing Li Pengqian Luan Lele Dong Jianqiao Liu Luying Jiang Jing Bai Fufeng Liu Yanjun Jiang School of Chemical Engineering and Technology Hebei University of TechnologyTianjin 300130China College of food Science and Biology Hebei University of Science&Technology26 Yuxiang StreetYuhua DistrictShijiazhuang 050018China Key Laboratory of Industrial Fermentation microbiology of Ministry of Education Tianjin Key Laboratory of Industrial MicrobiologyCollege of BiotechnologyTianjin University of Science&TechnologyTianjin 300457China

Asymmetric reduction of the conjugated C=C bonds by the old yellow enzymes(OYEs)presents a promising field in the synthesis of chiral ***,few natural OYEs have been applied in large-scale applications due to the requirement of costly NADPH and low operational ***,a stable and efficient fusion of YqjM from Bacillus subtilis and glucose dehydrogenase(GDH)from Bacillus megaterium was constructed to stereoselectively reduce the conjugated C=C bonds in a self-sufficient continuous *** effects of the enzyme order and different linkers on the fusions were investigated by structural analysis and all-atom molecular dynamics *** best fusion YqjM_G_GDH gave 98% conversion of 100 mmol/L 2-methylcyclopentenone with an excellent ee value(>99%)in 3 h,while the mixture of individual enzymes only obtained 68% conversion after more than 8 *** improved substrate conversion of YqjM_G_GDH fusion was probably attributed to the increased flexibility of each fused enzyme and the shortening of the diffusion distance of NADPH regenerated.A one-pot process was designed to purify and immobilize the fusion on the Ni2t-nitrilotriacetic acid functionalized magnetic mesoporous silica *** resulting immobilized biocatalyst not only catalyzed the asymmetric reduction of various α,β-unsaturated ketones(20 mmol/L)continuously with only 50μmol/L NADPt to initiate the whole process,but also retained more than 82%of the initial activity after seven cycles,serving as a good candidate for the industrial applications.

关键词： Enzyme fusion Old yellowenzymes Asymmetricreduction C=C bond Cofactor regeneration Immobilization

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Mechanistic insights toward identification and interaction of plant parasitic nematodes:A review

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Soil Ecology Letters 2024年第1期6卷 11-27页

作者： Bisma Jan Ali Haider Shah Mudasir Ahmad Bhat Arif Tasleem Jan Ishfaq Ahmad Wani Ali Asghar Shah Section of microbiology Baba Ghulam Shah Badshah UniversityRajouriJ&K185234India department of biotechnology Baba Ghulam Shah Badshah University RajouriJ&K185234India department of Botany Baba Ghulam Shah Badshah University RajouriJ&K185234India department of Zoology School of Biosciences and BiotechnologyBaba Ghulam Shah Badshah University RajouriJ&K185234India

Nematode identification serves as an important param-eter to study their behaviour,importance and *** of classical morphometric based identifica-tion methods prove to be lacking due to insufficient knowledge on morphological variations among closely related *** approaches such as DNA and protein-based information,microarray,probing,sequence-based methods and others have been used to supplement morphology-based methods for nematode *** and certain protein-based nematode-associated molecular patterns(NAMPs),can be perceived by the host plants,and can initiate a signalling *** review primarily emphasizes on an updated account of different classical and modern tools used for the identification of *** we also summa-rize the mechanism of some important signalling pathways which are involved in the different plant nematode *** constitute most diverse and least studied group of soil inhabiting *** are ecologically and physiologically important,however,wide range of nematodes show harmful impact on the individuals that live within their *** parasitic nematodes(PPNs)are transparent,pseudocoelomate,free living or parasitic *** lack morphometric identification methods due to insufficient knowl-edge on morphological variations among closely related *** such,molecular approaches such as DNA and protein-based information,microarray,probing,sequence-based methods and others have been used to supplement morphology-based methods for their *** invade the defense response of different plant species,parasitic nematodes have evolved different molecular *** and certain protein-based nematode-associated molecular patterns(NAMPs),can be perceived by the host plants,and can initiate a signaling *** overcome the host confrontation and develop certain nematode feeding sites,some members can inject effectors into

关键词： nematode identification plant nematode interaction DNA fingerprinting nematode-associated molecular patterns signaling path-ways

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Bifidobacterium breve alleviates gut–liver-axis injury caused by high-fat diet and aflatoxin B1 in mice

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food Science and Human Wellness 2024年

作者： Yongli Ye Tingwei Wang Jian Ji Jiasheng Wang Yinzhi Zhang Xiulan Sun International Joint Laboratory on food Safety， Synergetic Innovation Center of food Safety and Quality Control， School of food Science and Technology， Jiangnan University School of Biological and Chemical Engineering， Zhejiang University of Science and Technology Yixing Institute of food and biotechnology Co.， Ltd. department of Environmental Health Science， College of Public Health， University of Georgia

Aflatoxin B1(AFB1) is a common contaminant in cereals of global concern， and long-term low-dose exposure can adversely affect human health. Here， we showed that populations with dietary patterns characterized by high-fat diet (HFD) might have an increased risk of exposure to high levels of AFB1. Our data indicated that chronic exposure of AFB1 induced “gut-liver axis” injury in mice under HFD and normal diet (ND) patterns. AFB1 further aggravated hepatic and intestinal injury， and intestinal microbiota disruption in HFD mice. Bifidobacterium breve BAA-2849 intervention analysis showed that liver injury and lipid disorders caused by AFB1 exposure were alleviated by regulating the proportions of different gut microbes. We demonstrated through a mice model that the populations with a dietary pattern of HFD might be more susceptible to AFB1 exposure and adverse effects on the gut-liver axis， and the toxicity of AFB1 exposure can be alleviated by regulating the gut microbiota.

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