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Development of Rescue Material Transport UAV (Unmanned Aerial Vehicle)

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World Journal of engineering and Technology 2017年第4期5卷 720-729页

作者： Daeil Jo Yongjin Kwon department of Industrial engineering College of Engineering Ajou University Suwon South Korea

Recently, the market for drones is growing rapidly. Commercial UAVs (Unmanned Aerial Vehicles, or drones) are increasingly being used for various purposes, such as geographic survey, rescue missions, inspection of industrial facilities, traffic monitoring and delivery of cargos and goods. In particular, the drones have great potential for life-saving operations. A missing person, for example, can be rapidly and effectively searched using a drone in comparison with the conventional search operations. However, there is no commercially available rescue UAV until now. The motivation for this study is to design an unmanned aerial vehicle capable of vertical takeoff and landing, while containing a high power propellant apparatus in order to lift a heavy cargo that contains rescue materials (such as water, food, and medicine). We used the EDF (Electric Ducted Fan) technology as opposed to the conventional motor and prop combination. The EDF can produce the power about three times higher than the motor-prop combination. This became suitable for transportation of rescue goods, and can be widely used in rescue operations in natural environments. Based on these results, the UAV for rescue material transport capable of heavy vertical takeoff and landing is developed, including airframe, flight control computer and GCS (ground control station).

关键词： High-Powered Propellant Vertical Take-Off and Landing Rescue Drone GCS (Ground Control Station) FCC (Flight Control Computer)

Neural plasticity and adult neurogenesis: the deep biology perspective

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Neural Regeneration Research 2019年第2期14卷 201-205页

作者： Anna Maria Colangelo Giovanni Cirillo Lilia Alberghina Michele Papa Hans V.Westerhoff Laboratory of Neuroscience "R.Levi-Montalcini" Dept.of Biotechnology and Biosciences University of Milano-Bicocca SYSBIO Centre of Systems Biology University of Milano-Bicocca NeuroMI Milan Center for Neuroscience University of Milano-Bicocca Laboratory of Morphology of Neuronal Network Department of Public Medicine University of Campania "Luigi Vanvitelli" Synthetic Systems Biology and Nuclear Organization University of Amsterdam Molecular Cell Physiology VU University Amsterdam and Infrastructure Systems Biology at NL (ISBE.NL) Amsterdam NL and Systems Biology School for Chemical Engineering and Analytical Science University of Manchester

The recognition that neurogenesis does not stop with adolescence has spun off research towards the reduction of brain disorders by enhancing brain regeneration. Adult neurogenesis is one of the tougher problems of developmental biology as it requires the generation of complex intracellular and pericellular anatomies, amidst the danger of neuroinflammation. We here review how a multitude of regulatory pathways optimized for early neurogenesis has to be revamped into a new choreography of time dependencies. Distinct pathways need to be regulated, ranging from neural growth factor induced differentiation to mitochondrial bioenergetics, reactive oxygen metabolism, and apoptosis. Requiring much Gibbs energy consumption, brain depends on aerobic energy metabolism, hence on mitochondrial activity. Mitochondrial fission and fusion, movement and perhaps even mitoptosis, thereby come into play. All these network processes are interlinked and involve a plethora of molecules. We recommend a deep thinking approach to adult neurobiology.

关键词： neurogenesis adult brain neuroregeneration neuron differentiation nerve growth factor energy homeostasis mitochondria deep biology systems biology

Chelerythrine chloride from Macleaya cordata induces growth inhibition and apoptosis in human gastric cancer BGC-823 cells

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Acta Pharmaceutica Sinica B 2012年第5期2卷 464-471页

作者： Zhengfu Zhang Ying Guo Lingwei Zhang Jianbin Zhang Xionghui Wei department of Applied Chemistry College of Chemistry&Molecular EngineeringPeking UniversityBeijing 100871China Center for Drug Certification State Food and Drug AdministrationBeijing 100061China department of Chemical Biology School of Pharmaceutical SciencesPeking UniversityBeijing 100083China

This study aimed to investigate the ability of chelerythrine chloride(CHE),the main active ingredient of Macleaya cordata,to induce apoptosis in human gastric cancer BGC-823 *** results demonstrate that CHE inhibits cell proliferation in a time-and dose-dependent manner with accompanying S phase *** also induces apoptosis by a mechanism involving a reduction in the mitochondrial mem brane potential,the release of cytochrome c,activation of caspase 3 and cleavage of poly-ADP-ribose *** addition,CHE-induced apoptosis is accompanied by down-regulation of Bcl-x1 and Bcl-2 proteins with no change in the levels of Bax *** together,the results support the development of CHE as a potentially useful anticancer drug for the treatment of gastric cancer.

关键词： Macleaya cordata Chelerythrine chloride BGC-823 cells Apoptosis

Technique of Earthworms Restoring Soil in Greenhouse Cultivation

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Asian Agricultural Research 2024年第1期16卷 40-43页

作者： Yanjiao LI Jiafei SU Zhiu ZHANG Guang QI Jianhua CHEN Lixuan KOU Limin WANG Wenxian LIU Junyi ZHANG Libing QIU School of Chemical and Environmental engineering Pingdingshan UniversityPingdingshan 467000China

The production environment of greenhouse cultivation is relatively closed,the multiple cropping index is high,the management of fertilizationwatering and pesticideapplication isblindtosomeextent,andthe phenomenonofcontinuous cropping isalsocommonSoilquali-ty affects the sustainable development of greenhouse *** is a ubiquitous invertebrate organism in soil,an important part of soil system,a link between terrestrial organisms and soil organisms,an important link in the small cycle of soil material organisms,and plays an important role in maintaining the structure and function of soil *** ecotypes of earthworms are closely related to their habi-tats(soil layers)and food resource preferences,and then affect their ecological *** principle of earthworm regulating soil function is essentially the close connection and interaction between earthworm and soil *** different ecotypes of earthworms and bio-logical agents to carry out combined remediation of greenhouse cultivation soil is a technical model to realize sustainable development of green-house cultivation.

关键词： Earthworms Greenhouse cultivation Soil remediation Biological agent

Phosphorylation and function of DGAT1 in skeletal muscle cells

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Biophysics Reports 2015年第1期1卷 41-50页

作者： Jinhai Yu Yiran Li Fei Zou Shimeng Xu Pingsheng Liu National Laboratory of Biomacromolecules Institute of BiophysicsChinese Academy of Sciences University of Chinese Academy of Sciences department of Biological Science and biotechnology School of Biological Science and Medical EngineeringBeihang University Graduate School of Anhui Medical University General Hospital of Air Force

Aberrant intramuscular triacylglycerol(TAG) storage in human skeletal muscle is closely related to insulin insensitivity. Excessive lipid storage can induce insulin resistance of skeletal muscle, and under severe conditions, lead to type 2 diabetes. The balance of interconversion between diacylglycerol and TAG greatly influences lipid storage and utilization. Diacylglycerol O-acyltransferase 1(DGAT1) plays a key role in this process, but its activation and phosphorylation requires further dissection. In this study,12 putative conserved phosphorylation sites of DGAT1 were identified by examining amino acid conservation of DGAT1 in different species. Another 12 putative phosphorylation sites were also found based on bioinformatics predictions and previous reports. Meanwhile, several phosphorylation sites of DGAT1 were verified by phosphorylation mass spectrometry analysis of purified DGAT1 from mouse myoblast C2 C12 cells. Using single point mutations, a regulatory role of 3 putative phosphorylation sites was dissected. Finally, using truncation mutations, a potential domain of DGAT1 that was involved in the regulation of enzymatic activity was revealed. This study provides useful information for further understanding DGAT1 activity regulation.

关键词： DGAT1 Enzymatic activity Phosphorylation Single point mutation

Improving the quality of cold storage black tiger prawn （Penaeus monodon） using ozonated water

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Improving the quality of cold storage black tiger prawn （Pe...

Targeting Glycinebetaine for Abiotic Stress Tolerance in Crop Plants:Physiological Mechanism,Molecular Interaction and Signaling

International Conference on biotechnology and food Science（ICBFS 2011）

作者： Sumate Tantratian Jirapa Setjintanin Romanee Sanguandeekul department of food Technology Faculty of ScienceChulalongkorn UniversityBangkokThailand

The ozonated water with total ozone concentration （TOC）220-1130 mg/l or residual ozone concentration（ROC） less than 0.1-0.31 mg/l were evaluated against total bacteria and psychrotrophic *** was found that antimicrobial effect increased when the concentration of ozone increased for the cell suspension and on the shrimp *** storage at 4 ℃,the shrimps washed with water and ozonated water did not have sensory scores significantly different（P≥0.05）in color and *** TVB and pH of the shrimp meat were significantly lower than the water *** ozonated water 1130 mg/l could reduce the number of bacteria on the shrimp and also retarded the growth of psychrotropic *** ozonated water could prolong the detectable bad odor for more than 4 days.

关键词： black tiger prawn cold storage quality ozonated water

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Phyton-International Journal of Experimental Botany 2019年第3期88卷 185-221页

作者： Mirza Hasanuzzaman Aditya Banerjee M.H.M.Borhannuddin Bhuyan Aryadeep Roychoudhury Jubayer Al Mahmud Masayuki Fujita department of Agronomy Faculty of AgricultureSher-e-Bangla Agricultural UniversityDhakaBangladesh Post Graduate department of biotechnology St.Xavier’s College(Autonomous)30Mother Teresa SaraniKolkataWest BengalIndia Laboratory of Plant Stress Responses Department of Applied Biological ScienceFaculty of AgricultureKagawa University2393 IkenobeMiki-choKita-gunKagawaJapan Bangladesh Agricultural Research Institute JoydebpurGazipurBangladesh department of Agroforestry and Environmental Science Faculty of AgricultureSher-e-Bangla Agricultural UniversityDhakaBangladesh

In the era of climate change,abiotic stresses(e.g.,salinity,drought,extreme temperature,flooding,metal/metalloid(s),UV radiation,ozone,etc.)are considered as one of the most complex environmental constraints that restricts crop production *** of stress-tolerant crop cultivars is the most auspicious way of surviving this constraint,and to produce these types of tolerant *** bioengineering mechanisms involved in stress signaling are being adopted in this *** example of this kind of manipulation is the osmotic *** quarternary ammonium compound glycinebetaine(GB),also originally referred to as betaine is a methylated glycine *** the betaines,GB is the most abundant one in plants,which is mostly produced in response to dehydration caused by different abiotic stresses like drought,salinity,and extreme *** helps in decreased accumulation and detoxification of ROS,thereby restoring photosynthesis and reducing oxidative *** takes part in stabilizing membranes and *** is also involved in the stabilization and protection of photosynthetic components,such as ribulose-1,5-bisphosphate carboxylase/oxygenase,photosystem II and quarternary enzyme and protein complex structures under environmental *** was found to perform in chaperone-induced protein *** addition,GB can confer stress tolerance in very low concentrations,and it acts in activating defense responsive genes with stress ***,field application of GB has also shown protective effects against environmental adversities increasing crop yield and *** this review,we will focus on the role of GB in conferring abiotic stress tolerance and the possible ways to engineer GB biosynthesis in plants.

关键词： Osmolytes compatible solutes biostimulants proline amino acids stress signaling

Near index matching enables solid diffractive optical element fabrication via additive manufacturing

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Light(Science & Applications) 2023年第10期12卷 2187-2198页

作者： Reut Orange kedem Nadav Opatovski Dafei Xiao Boris Ferdman Onit Alalouf Sushanta Kumar Pal Ziyun Wang Henrik von der Emde Michael Weber Steffen J.Sahl Aleks Ponjavic Ady Arie Stefan WHell Yoav Shechtman Russell Berrie Nanotechnology Institute Technion—Israel Institute of TechnologyHaifaIsrael Lorry Lokey Interdisciplinary Center for Life Sciences and engineering Technion—Israel Institute of TechnologyHaifaIsrael department of Biomedical engineering Technion—Israel Institute of TechnologyHaifaIsrael School of Electrical engineering Fleischman Faculty of engineering Tel Aviv UniversityTel Aviv 69978Israel School of Physics and Astronomy University of LeedsLeedsUK School of food Science and Nutrition University of LeedsLeedsUK department of NanoBiophotonics Max Planck Institute for Multidisciplinary SciencesGöttingenGermany department of Optical Nanoscopy Max Planck Institute for Medical ResearchHeidelbergGermany

Diffractive optical elements(DOEs)have a wide range of applications in optics and photonics,thanks to their capability to perform complex wavefront shaping in a compact ***,widespread applicability of DOEs is still limited,because existing fabrication methods are cumbersome and ***,we present a simple and cost-effective fabrication approach for solid,high-performance *** method is based on conjugating two nearly refractive index-matched solidifiable transparent *** index matching allows for extreme scaling up of the elements in the axial dimension,which enables simple fabrication of a template using commercially available 3D printing at tens-of-micrometer *** demonstrated the approach by fabricating and using DOEs serving as microlens arrays,vortex plates,including for highly sensitive applications such as vector beam generation and super-resolution microscopy using MINSTED,and phase-masks for three-dimensional single-molecule localization *** the advantage of making DOEs widely accessible by drastically simplifying their production,the method also overcomes difficulties faced by existing methods in fabricating highly complex elements,such as high-order vortex plates,and spectrum-encoding phase masks for microscopy.

关键词： matching enable vortex

Effect of Limited Enzymatic Hydrolysis on Structure Characterization of Glycinin

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Agricultural biotechnology 2012年第3期1卷 52-54页

作者： Chunhong DUAN Wan SUN Yunjie LI Yunlong WANG Siyi PAN department of Bioengineering Wuhan Bioengineering Institute College of food Science and Technology Huazhong Agricultural University

[ Objective] This study aimed to characterize the structure of soybean glycinin affected by limited enzymatic hydrolysis. [ Method ] The glycinin was limitedly hydrolyzed by alkaline protease; then the SDS-polyacrylamide gel electrophoresis （SDS-PAGE）, scanning electron microscopy （SEM）, Fourier transform infrared spectroscopy （fTIR） and other means were performed to characterize the glyeinin structure changing during the hydrolysis process. [ Result] SDS-PAGE analysis showed that the subunit contem of glycinin significantly decreased after hydrolysis, and acidic subunits were more susceptible to hydrolysis than alkaline subunits. The scanning electron microscopy revealed that the structure of glycinin powder changed greatly after hydrolysis. The I^TIR results showed that the propor- tions of all conformations of glycinin changed greatly during hydrolysis process. In addition, the protein hydrophobicity and sulfhydryl content were also significantly influenced by hydrolysis. [ Conclusion] The enzymatic hydrolysis greatly changed the conformations of glyeinin, and the change was dependent on the degree of hy- drolysis.

关键词： Glycinin Alkaline proteinase Hydrolysis Structure characterization