检索结果-南通市图书馆

engineering and characterization of dehalogenase enzymes from Delftia acidovorans in bioremediation of perfluorinated compounds

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Synthetic and Systems biotechnology 2022年第2期7卷 683-688页

作者： Jackson D.Harris Collin M.Coon Megan E.Doherty Eamon A.McHugh Margaret C.Warner Conley L.Walters Olivia M.Orahood Abigail E.Loesch David C.Hatfield John C.Sitko Erin A.Almand J.Jordan Steel department of Biology United Stated Air Force AcademyColorado SpringsCO80840USA department of Biochemistry United States Air Force AcademyColorado SpringsCO80840USA department of Civil engineering United States Air Force AcademyColorado SpringsCO80840USA

Per-and Polyfluorinated alkyl substances(PFAS)are a broad class of synthetic compounds that have fluorine substituted for hydrogen in several or all locations and are globally categorized as PFCs(perfluorochemicals;commonly called fluorinated chemicals).These compounds have unique chemical and physical properties that enable their use in non-stick surfaces,fire-fighting efforts,and as slick ***,recent concerns over the health effects of such compounds,specifically perfluorooctanoic acid and perfluorooctane sulfonic acid(PFOA,PFOS;PFOA/S),have led to increased attention and research by the global community into degradation *** this study,soil samples from PFAS-contamination sites were cultured and screened for microbes with PFOA/S degradation potential,which led to the identification of Delftia *** was found that *** isolated from PFAS-contaminated soils was capable of growth in minimal media with PFOA as a sole carbon resource,and an observable fluoride concentration increase was observed when cells were exposed to *** suggests potential activity of a dehalogenase enzyme that may be of use in PFOA or PFAS microbial remediation *** associated haloacid dehalogenases have been identified in the *** genome and have been engineered for expression in Escherichia coli for rapid production and *** enzymes have shown potential for enzymatic defluorination,a significant step in biological degradation and removal of PFOA/S from the *** hypothesize that bioremediation of PFAS using naturally occurring microbial degradation pathways may represent a novel approach to remove PFAS contamination.

关键词： iGEM PFAS Bioremediation Bioengineering Delftia acidovorans

Carbon nanomaterials for cardiovascular theranostics:Promises and challenges

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bioactive Materials 2021年第8期6卷 2261-2280页

作者： Keshav Narayan Alagarsamy Sajitha Mathan Weiang Yan Alireza Rafieerad Saravanan Sekaran Hanna Manego Sanjiv Dhingra Regenerative Medicine Program Institute of Cardiovascular SciencesSt.Boniface Hospital Albrechtsen Research CentreDepartment of Physiology and PathophysiologyCollege of MedicineFaculty of Health SciencesUniversity of ManitobaWinnipegCanada Centre for Nanotechnology&Advanced Biomaterials(CeNTAB) Department of BioengineeringSchool of Chemical and BiotechnologySASTRA UniversityThanjavur613401Tamil NaduIndia Section of Cardiac Surgery Department of SurgeryMax Rady College of MedicineRady Faculty of Health SciencesUniversity of ManitobaWinnipegCanada

Cardiovascular diseases(CVDs)are the leading cause of death *** attack and stroke cause irreversible tissue *** currently available treatment options are limited to“damage-control”rather than tissue *** recent advances in nanomaterials have offered novel approaches to restore tissue function after *** particular,carbon nanomaterials(CNMs)have shown significant promise to bridge the gap in clinical translation of biomaterial based *** family of carbon allotropes(including graphenes,carbon nanotubes and fullerenes)have unique physiochemical properties,including exceptional mechanical strength,electrical conductivity,chemical behaviour,thermal stability and optical *** intrinsic properties make CNMs ideal materials for use in cardiovascular *** review is focused on recent efforts in the diagnosis and treatment of heart diseases using graphenes and carbon *** first section introduces currently available derivatives of graphenes and carbon nanotubes and discusses some of the key characteristics of these *** second section covers their application in drug delivery,biosensors,tissue engineering and immunomodulation with a focus on cardiovascular *** final section discusses current shortcomings and limitations of CNMs in cardiovascular applications and reviews ongoing efforts to address these concerns and to bring CNMs from bench to bedside.

关键词： Carbon nanomaterials Cardiovascular disease Drug delivery Biosensors Cardiac tissue engineering Immunomodulation

Comparison of wheat-based rotation systems and monocropping systems under dryland Mediterranean conditions

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Agricultural and Biological engineering 2017年第5期10卷 203-213页

作者： Servet Tekin Attila Yazar Hatun Barut department of Biosystems engineering Faculty of AgricultureKahramanmaras Sütcüİmam UniversityKahramanmaras 46100Turkey Irrigation and Agricultural Structures department Çukurova UniversityAdana 01330 Turkey Eastern Mediterranean Agricultural Research Institute Adana 01370Turkey

Mono-cropping systems consisting of general low-yielding cereals are crucial productivity constraints in dry areas of the Mediterranean region.A crop rotation consisting of quinoa(Chenopodium quinoa Willd.)-wheat(Triticum aestivum L)-chickpea(Cicer arietinum)was compared with monocropping in the Mediterranean region of Turkey.A four year crop rotation trial was set up in Adana,Turkey.A total of nine crop rotation systems of Quinoa(Q),chickpea(L)and wheat(W)were considered in this *** four year results revealed that there was significant difference in grain and biomass yields,but no significant difference in plant height,harvest index,and 1000 grain weight of wheat among the treatments in crop rotation except number of grains per spike and soil organic *** wheat and chickpea-wheat-chickpea-wheat(LWLW)produced significantly greater grain yield in 2010/2011 and 2011/*** and QWQW rotation produced significantly lower biomass yield in 2008/*** maximum biomass yield of LWLW rotation resulted in 2011/2012 growing ***,the effect of previous crop on wheat grain and biomass yield in the four years of the rotation study was significantly *** the long run,the favorable effects of legume-based rotations on crop yields and water productivity are apparent.

关键词： crop rotation system monocropping system quinoa Chenopodium quinoa chickpea dryland yield

Rheostatic Control of ABA Signaling through HOS15-Mediated OST1 Degradation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2019年第11期12卷 1447-1462页

作者： Akhtar Ali Jae Kyoung Kim Masood Jan Haris Ali Khan Irfan Ullah Khan Mingzhe Shen Junghoon Park Chae Jin Lim Shah Hussain Dongwon Baek Kai Wang Woo Sik Chung Vicente Rubio Sang Yeol Lee Zhizhong Gong Woe Yeon Kim Ray ABressan Jose MPardo Dae-Jin Yun department of Biomedical Science&engineering Konkuk UniversitySeoul 05029South Korea department of Mathematical Sciences Korea Advanced Institute of Science and TechnologyDaejeon 34131Korea Division of Applied Life Science Plant Molecular Biology and Biotechnology Research CenterGyeongsang National UniversityJinju 660-701South Korea State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China Centro Nacional de Biotecnologia-CSIC Darwin 3.Campus de la UAM.Cantoblanco28049 MadridSpain department of Horticulture and Landscape Architecture Purdue University625 Agriculture Mall DriveWest LafayetteIN 47907-2010USA lnstituto de Bioquimica Vegetal y Fotosintesis cicCartujaCSIC-Universidad de SevillaAmericoVespucio 49Sevilla 41092Spain

Dehydrating stresses trigger the accumulation of abscisic acid(ABA),a key plant stress-signaling hormone that activates Snf1-Related Kinases(SnRK2s)to mount adaptive ***,the regulatory circuits that terminate the SnRK2s signal relay after acclimation or post-stress conditions remain to be ***,we show that the desensitization of the ABA signal is achieved by the regulation of OST1(SnRK2.6)protein stability via the E3-ubiquitin ligase *** ABA signal,HOS15-induced degradation of OST1 is inhibited and stabilized OST1 promotes the stress *** the ABA signal terminates,protein phosphatases ABI1/2 promote rapid degradation of OST1 via ***,we found that even in the presence of ABA,OST1 levels are also depleted within hours of ABA signal *** unexpected dynamics of OST1 abundance are then resolved by systematic mathematical modeling,demonstrating a desensitizing feedback loop by which OST1-induced upregulation of ABI1/2 leads to the degradation of *** model illustrates the complex rheostat dynamics underlying the ABA-induced stress response and desensitization.

关键词： ABA signaling drought stress HOS15 OST1 ABI1/2 protein degradation and stability

Inhibition of mycotoxin deoxynivalenol generation by using selenized glucose

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2020年第12期31卷 3276-3278页

作者： Kueyun Mao Peizi Li Tao Li Minmeng Zhao Chao Chen Jian Liu Zhiqiang Wang Lei Yu Key Laboratory of Plant Functional Cenortis of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Collaborative Innovarion of Modern Crops and food Crops in Jiangsu/Jiangsu Key Laboratory of Crop Cenetics and Physiology College of AgricultureYangzhou UniversityYangzhou 225009China School of Chemistry and Cherrical engineering Yangzhou UniversityYangzhou 225002China College of Aninal Science and Technology Yangzhou UniversityYangzhou 225009China department of Applied Chemistry Faculty of EngineeringKyushu Institute of TechnologyKitakyushu 804-8550Japan Sichuan Selewood Technolagy Compary Limited Chengdu 610218China College of Vererinary Medicine Yanagzhou UniversiryYangzhou 225009China

Selenized glucose can be easily prepared via the selenization reaction of glucose using in situ generated NaHSe as the selenization *** technique has been industrialized to produce the chemical in kilogram scale,making it an easily available mate rial in laborato ry *** selenized glucose may be widely used as the starting material for the preparation of selenium-containing catalysts,as the organoselenium additive for feeds,and as the efficient selenium-enriched foliar *** this work,we found that treating Fusarium graminearum,a fungal pathogen inciting wheat scab disease,with selenium glucose could significantly inhibit the generation of the deoxynivalenol(DON)toxin,which might be a breakthrough for reducing the detriment of the wheat scab disease.

关键词： Wheat scab disease Deoxynivalenol Selenium Bioactivity

Assessment of Expression Cassettes and Culture Media for Different Escherichia coli Strains to Produce Astaxanthin

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Natural Products and Bioprospecting 2018年第5期8卷 397-403页

作者： Shun Li Jun-Chao Huang department of Economic Plants and biotechnology Yunnan Key Laboratory for Wild Plant ResourcesKunming Institute of BotanyChinese Academy of SciencesKunming 650201People’s Republic of China University of Chinese Academy of Sciences Beijing 100049People’s Republic of China

Astaxanthin is a value-added ketocarotenoid with great potential in nutraceutical and pharmaceutical *** engineering of heterologous hosts for astaxanthin production has attracted great *** this study,we assessed some key factors,including codon usage of the expressed genes,types of promoters,bacterial strains,and culture media,for engineered Escherichia coli to produce *** effect of codon usage was shown to be related to the types of *** DH5a was superior to other strains for astaxanthin *** culture media greatly affected the contents and yields of astaxanthin in engineered *** the expression cassette containing GadE promoter and its driving genes,HpCHY and CrBKT,was inserted into the plasmid pACCAR16DcrtX and expressed in *** DH5a,the engineered strain was able to produce 4.30±0.28 mg/g dry cell weight(DCW)or 24.16±2.03 mg/L of astaxanthin,which was a sevenfold or 40-fold increase over the initial production of 0.62±0.03 mg/g DCW or 0.61±0.05 mg/L.

关键词： Astaxanthin Escherichia coli Bacterial strains Codon usage Medium

Monoclonal antibodies:Principles and applications of immmunodiagnosis and immunotherapy for hepatitis C virus

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Hepatology 2015年第22期7卷 2369-2383页

作者： Ashraf Tabll Aymn T Abbas Sherif El-Kafrawy Ahmed Wahid Microbial biotechnology department (Biomedical Technology Group) Genetic Engineering and Biotechnology DivisionNational Research Centre biotechnology Research Laboratories Gastro--eneterology Surgery CentreMansoura University Special Infectious Agents Unit King Fahd Medical Research CenterKing Abdulaziz University National Liver Institute Menoufia University Biochemistry department Molecular and Cellular Biology UnitFaculty of PharmacyMinia University

Hepatitis C virus(HCV) is a major health problem worldwide. Early detection of the infection will help better management of the infected cases. The monoclonal antibodies(m Ab) of mice are predominantly used for the immunodiagnosis of several viral,bacterial,and parasitic antigens. Serological detection of HCV antigens and antibodies provide simple and rapid methods of detection but lack sensitivity specially in the window phase between the infection and antibody development. Human mA b are used in the immunotherapy of several blood malignancies,such as lymphoma and leukemia,as well as for autoimmune diseases. In this review article,we will discuss methods of mouse and human monoclonal antibody production. We will demonstrate the role of mouse mA b in the detection of HCV antigens as rapid and sensitive immunodiagnostic assays for the detection of HCV,which is a major health problem throughout the world,particularly in Egypt. We will discuss the value of HCV-neutralizing antibodies and their roles in the immunotherapy of HCV infections and in HCV vaccine development. We will also discuss the different mechanisms by which the virus escape the effect of neutralizing mA b. Finally,we will discuss available and new trends to produce antibodies,such as egg yolk-based antibodies(Ig Y),production in transgenic plants,and the synthetic antibody mimics approach.

关键词： Hepatitis C virus Monoclonal antibodies Immunodiag

Untargeted metabolomics characteristics of nonobese nonalcoholic fatty liver disease induced by high-temperature-processed feed in Sprague-Dawley rats

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2020年第46期26卷 7299-7311页

作者： Li-Jun Xue Ju-Qiang Han Yuan-Chen Zhou Hong-Ye Peng Teng-Fei Yin Kai-Min Li Shu-Kun Yao School of Biological Science and Medical engineering Beihang UniversityBeijing 100191China department of Hepatology The Seventh Medical Center of PLA General HospitalBeijing 100700China department of Gastroenterology China-Japan Friendship HospitalBeijing 100029China

BACKGROUND Nonalcoholic fatty liver disease(NAFLD)has become one of the most common chronic liver diseases in the *** our early clinical data and questionnaire analysis of NAFLD,it was found that the body mass index of some patients did not meet the diagnostic criteria for overweight or *** consumption of high-temperature-processed foods such as fried food,hot pot and barbecue is closely related to the occurrence of nonobese *** the intake of this kind of food can reduce disease severity and improve *** To explore the untargeted metabolomics characteristics of nonobese nonalcoholic fatty liver disease in Sprague-Dawley rats induced by high-temperatureprocessed *** Fifty-four male Sprague-Dawley rats were divided into three groups:The control group received a standard diet;the nonfried soybeans(NDFS)group received 60%NDFS and 40%basic feed and the dry-fried soybeans(DFS)group received 60%DFS and 40%basic *** rats were sacrificed at week 4,8,and 12 in each *** food intake,body weight,Lee’s index,liver index,serological index and hepatic histopathology were *** metabolomics characteristics were used to analyze the changes in liver metabolites of rats at week *** between metabolites and pathology scores between the DFS and control groups and between the DFS and NDFS groups were *** selected some of the metabolites,both within the pathway and outside of the pathway,to explain preliminarily the difference in liver pathology in the three groups of *** There were no statistically significant differences in the food intake,body weight,Lee's index or serological index between the DFS group and the control group(P>0.05).At week 8 and week 12,the steatosis scores in the DFS group were significantly higher than those in the other two groups(P<0.05).At week 12,the liver index of the DFS group was the lowest(NDFS group vs DFS group,P<0.05).The fibrosis score in the DFS group was sign

关键词： Nonobese nonalcoholic fatty liver disease High-temperature-processed feed Mild steatosis and early fibrosis Untargeted metabolomics characteristics Animal models Novel pathogenesis for nonalcoholic fatty liver disease

Nuciferine protects against high-fat diet-induced hepatic steatosis and insulin resistance via activating TFEB-mediated autophagy——lysosomal pathway

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2022年第6期12卷 2869-2886页

作者： Xiliang Du Chiara Di Malta Zhiyuan Fang Taiyu Shen Xiaodi Niu Meng Chen Bo Jin Hao Yu Lin Lei Wenwen Gao Yuxiang Song Zhe Wang Chuang Xu Zhijun Cao Guowen Liu Xinwei Li Key Laboratory of Zoonosis Ministry of EducationCollege of Veterinary MedicineJilin UniversityChangchun 130062China Telethon Institute of Genetics and Medicine(TIGEM)Via Campi Flegrei 34 PozzuoliNA 80078Italy Medical Genetics Unit Department of Medical and Translational ScienceFederico Ⅱ UniversityNaples 80131Italy College of food Science and engineering Jilin UniversityChangchun 130062China College of Animal Science and Veterinary Medicine Heilongjiang Bayi Agricultural UniversityDaqing 163319China State Key Laboratory of Animal Nutrition Beijing Engineering Technology Research Center of Raw Milk Quality and Safety ControlCollege of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China

Nonalcoholic fatty liver disease(NAFLD) is characterized by hepatic steatosis and insulin resistance and there are currently no approved drugs for its *** of mTOR complex1(mTORCl) and subsequent impairment of the transcription factor EB(TFEB)-mediated autophagy-lysosomal pathway(ALP) are implicated in the development of ***,agents that augment hepatic TFEB transcriptional activity may have therapeutic potential against *** objective of this study was to investigate the effects of nuciferine,a major active component from lotus leaf,on NAFLD and its underlying mechanism of *** we show that nuciferine activated ALP and alleviated steatosis,insulin resistance in the livers of NAFLD mice and palmitic acid-challenged hepatocytes in a TFEB-dependent *** investigation revealed that nuciferine interacts with the Ragulator subunit hepatitis B X-interacting protein and impairs the interaction of the Ragulator complex with Rag GTPases,thereby suppressing lysosomal localization and activity of mTORC1,which activates TFEB-mediated ALP and further ameliorates hepatic steatosis and insulin *** present results indicate that nuciferine may be a potential agent for treating NAFLD and that regulation of the mTORCl-TFEB-ALP axis could represent a novel pharmacological strategy to combat NAFLD.

关键词： Autophagy Fatty liver Lotus leaf Lysosome MiT/TFE mTORC1 Ragulator TFEB