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Acta Biochimica et Biophysica Sinica 2004年第11期36卷 773-779页

作者： Bo-Xiong ZHONG and Yan-Wei SHENdepartment of bioresource science, College of Animal science, Zhejiang University, Hangzhou 310029, China department of bioresource science College of Animal Science Zhejiang University Hangzhou 310029 Chin

Soluble proteins extracted from leaves, apical shoots, axillary shoots, and stems of garland chrysanthemum plants infected by onion yellows phytoplasma were analyzed by two-dimensional gel electrophoresis. Computerized matching analysis revealed that at least six soluble proteins were accumulated specifically in phytoplasma-infected garland chrysanthemum. N-terminal amino acids sequences of these soluble

关键词： phytoplasma garland chrysanthemum Chrysanthemum coronarium pathogenesis-related proteins thaumatin osmotin

Sneak-peek into the chlorophyll content, antioxidant activity, targeted and non-targeted UHPLC-QTOF LC/MS metabolomic fingerprints of pulse microgreens grown under different photoperiod regimes

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food Bioscience 2023年第2期52卷 1293-1302页

作者： Arun Kumar Narpinder Singh Amritpal Kaur Robin Joshi department of food science and Technology Guru Nanak Dev UniversityAmritsarPunjab143005India department of food science and Technology Graphic Era Deemed to be UniversityDehradun248002India department of Biotechnology CSIR-Institute of Himalayan Bioresource TechnologyPalampur176061HPIndia

Pulse microgreens(mung bean,chickpea and lentil)were evaluated for compositional changes as a function of *** pulse microgreens were cultivated under photoperiod of 16 and 22 h,with an optimum growing temperature of 22℃ in light and 17℃ in dark in polypropylene trays containing moist cotton *** photoperiodic response of pulse microgreens in terms of plant height,chlorophyll content,antioxidant activity,phenolic profile,sugar profile and secondary metabolites were undertaken as the experimental *** targeted phenolic and sugar profile varied significantly with *** various phenolic acids,especially chlorogenic acid and p-coumaric acid increased amongst different microgreens on extending *** increase in sugar and their derivatives reflected the upregulation of carbohydrate metabolism under longer ***,the plant height,total chlorophyll content and antioxidant activity of pulse microgreen increased significantly on prolonging the *** non-targeted analysis by UHPLC-QTOF LC/MS revealed the presence of secondary metabolites of five major classes,which were known to have beneficial effects in biological ***-glucoside,an organic compound was exclusively present in mung bean microgreens which further increased in longer *** extraordinary supply of secondary metabolites makes the pulse microgreens excellent ingredient for therapeutic functional foods.

关键词： Pulse microgreen Photoperiod Chlorophyll content Phenolic compounds Sugars Secondary metabolites

VOC removal from contaminated groundwater through membrane pervaporation. (Ⅱ): 1,1,1-trichloroethane-SDS surfactant solution system

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Journal of Environmental sciences 2003年第6期15卷 821-826页

作者： Sean LIU department of food science Rutgers University

The conventional “pump-and-treat' technology for subsurface remediation of groundwater contaminated with volatile organic compounds(VOCs) such as 1,1,1-trichloroethane(TCA), a common chlorinated organic solvent, has limitation of prohibitively long treatment time due to extremely low water solubility of the VOCs. Surfactant-based soil remediation has emerged as the effective technology that substantially reduces the treatment time. In order to make the whole process economical, the surfactant used in soil washing has to be recovered and reused. This study examined the recovery of anionic surfactant, sodium dodecyl sulfate(SDS), from soil remediation fluids containing TCA, using a bench-scale membrane pervaporation unit. The effects of high TCA concentration, surfactant dosage, and flow rate on permeation flux and selectivity(α value) of the process were evaluated. In general, higher surfactant concentration yielded lower TCA flux and constant water flux, resulting in declining α values; higher flow rate of TCA feed stream results in higher VOC flux and selectivity, an indication of the effect of concentration polarization; higher TCA feed concentration produces higher TCA permeation across the membrane, however, the selectivity was virtually unchanged unless the total TCA concentration exceeded 2000 ppm.

关键词： pervaporation VOCs SDS surfactant groundwater remediation 1,1,1-trichloroethane

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Role of copper chelating agents: between old applications and new perspectives in neuroscience

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Neural Regeneration Research 2025年第3期20卷 751-762页

作者： Rosalba Leuci Leonardo Brunetti Vincenzo Tufarelli Marco Cerini Marco Paparella Nikola Puvača Luca Piemontese department of Pharmacy-Pharmaceutical science University of Bari Aldo MoroBariItaly department of Precision and Regenerative Medicine and Jonian Area(DiMePRe-J) Section of Veterinary Science and Animal ProductionUniversity of Bari Aldo MoroBariItaly department of Engineering Management in Biotechnology Faculty of Economics and Engineering Management in Novi SadUniversity Business Academy in Novi SadNovi SadSerbia

The role of copper element has been an increasingly relevant topic in recent years in the fields of human and animal health, for both the study of new drugs and innovative food and feed supplements. This metal plays an important role in the central nervous system, where it is associated with glutamatergic signaling, and it is widely involved in inflammatory processes. Thus, diseases involving copper(Ⅱ) dyshomeostasis often have neurological symptoms, as exemplified by Alzheimer's and other diseases(such as Parkinson's and Wilson's diseases). Moreover, imbalanced copper ion concentrations have also been associated with diabetes and certain types of cancer, including glioma. In this paper, we propose a comprehensive overview of recent results that show the importance of these metal ions in several pathologies, mainly Alzheimer's disease, through the lens of the development and use of copper chelators as research compounds and potential therapeutics if included in multi-target hybrid drugs. Seeing how copper homeostasis is important for the well-being of animals as well as humans, we shortly describe the state of the art regarding the effects of copper and its chelators in agriculture, livestock rearing, and aquaculture, as ingredients for the formulation of feed supplements as well as to prevent the effects of pollution on animal productions.

关键词： agriculture Alzheimer's disease chelators copper feed supplements multi-target

BIOPOLYMER BASED NANO-DELIVERY SYSTEMS FOR ENHANCING BIOAVAILABILITY OF NUTRACEUTICALS

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Chinese Journal of Polymer science 2013年第9期31卷 1190-1203页

作者： Bing Hu Qing-rong Huang College of food science and Technology Nanjing Agricultural University department of food science Rutgers University

Recently, there has been an increasing interest in the development of efficient food-grade oral nano-delivery systems for encapsulation, protection and target delivery of nutraceuticals to enhance their bioavailability, fiarther to prevent disease and promote human health and well-being. food proteins represent promising candidates for efficient nutraceutical nanocarriers due to their exceptional characteristics, namely biodegradability, nonantigenicity, high nutritional value, abundant renewable sources and extraordinary binding capacity to various nutraceuticals. In addition, their biocompatibility, biodegradability, low toxicity, low cost, and non-starch polysaccharides possess many favourable characteristics such as stability in the harsh gastric environment, resistance to digestive enzymes, and mucoadhesiveness to intestinal mucosal surface. This review describes the design and formation of nanoscaled delivery systems for nutraceuticals using food-grade proteins （including peptides）, polysaccharides and their associative complexes. The toxicity and cellular uptake fate of the nanostructures, as well as their effects on the intestinal absorption of the encapsulated nutraceuticals were also discussed.

关键词： food biopolymer Nano-delivery systems Bioavailability Nutraceuticals.

Quantitative Structure-activity Relationship Studies on the Antioxidant Activity and Gap Junctional Communication of Carotenoids

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Chinese Journal of Structural Chemistry 2010年第9期29卷 1362-1372页

作者：孙玉敬吴丹刘东红陈健初沈妍叶兴乾 department of food science and Nutrition School of Biosystems Engineering and Food ScienceZhejiang University

The antioxidant and gap junctional communication（GJC） activities of carotenoids are known to be the two main anticancer *** structure-activity relationship（QSAR） models of the two activities were developed using stepwise regression and multilayer perceptron neural network based on the calculated descriptors of quantum *** results showed that the significant molecular descriptor related to the antioxidant activity of carotenoids was the HOMO-LUMO energy gap（EHL） and the molecular descriptor related to the GJC was the lowest unoccupied molecular orbital energy（ELUMO）.The two models of antioxidant activity both showed good predictive power,but the predictive power of the neural network QSAR model of antioxidant activity was *** addition,the two GJC models have similar,moderate predictive *** possible mechanisms of antioxidant activity and GJC of carotenoids were discussed.

关键词： carotenoids antioxidant activity gap junctional communication multilayer perceptron neural network quantitative structure-activity relationship

QSPR Study on the Glass Transition Temperature of Polyacrylates

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Chinese Journal of Structural Chemistry 2007年第12期26卷 1466-1470页

作者：刘天宝彭艳芬吴新民 department of Chemistry and food science Chizhou College

Structural parameters of 22 polyacrylic compounds were computed at two levels using Hartree-Fock and DFT methods. Based on the experimental data of glass transition temperature （Tg）, four-parameter （energy of the lowest unoccupied molecular orbital （ELoMO）, the highest positive charge （Qmax^＋）, dipole moments（μ） and the next highest occupied molecular orbital （ENLOMO）） dependent equations were developed using structural parameters as theoretical descriptors. Especially, Tg dependent equation calculated at the HF/6-31G（d） level is more advantageous than others in view of their correlation and predictive abilities. This dependent equation was validated by variance inflation factors （VIF） and t-test methods.

关键词： glass transition temperature QSPR polyacrylate Hartree-fock DFT

Effective extraction of elastase from Bacillus sp. fermentation broth using aqueous two-phase system

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Journal of Zhejiang University-science B(Biomedicine & Biotechnology) 2005年第11期6卷 1087-1094页

作者：徐莹何国庆李景军 department of food science and Nutrition Zhejiang University School of food science and Technology Southern Yangtze University

This paper presents the evaluation of an aqueous two-phase system （ATPS） for extracting elastase produced by Bacillus sp. EL31410. The elastase and cell partition behavior in polyethylene glycol （PEG）/salt systems was investigated. The suitable system for elastase extraction was PEG/KHEPO4-KEHPO4, in which elastase is mainly partitioned into the PEG-rich phase, while the cells remained in the other phase. The influence of defined system parameters （e.g. PEG molecular mass, pH, NaCl addition） on the partitioning behavior of elastase is described. The concentration of phase forming components, PEG and KHEPO4-KEHPO4, was optimized for elastase recovery by means of response surface methodology, and it was found that they greatly influenced extraction recovery. The optimal ATPS was 23.1% （w/w） PEG 2000 and 11.7% （w/w） KHEPO4-KEHPO4. The predicted recovery was about 89.5%, so this process is suggested to be a rapid and convenient method for elastase extraction.

关键词： Elastase Aqueous two-phase system (ATPS) Bioseparation Purification Optimization Response surface methodology (RSM)

Effects of Irradiation on the Structure-activity Relationship of Konjac Glucomannan Molecular Chain Membrane

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Chinese Journal of Structural Chemistry 2012年第6期31卷 857-866页

作者：吴春华彭述辉温成荣王丽霞熊波刘雅楠范琳琳姚闽娜庞杰 College of food science Fujian Agriculture and Forestry University department of food science Guangzhou City Polytechnic

To know the effects of irradiation on the konjac glucomannan （KGM） molecular chain membrane, KGM membrane solution was treated with the irradiation dose of 0-20 kGy in this study, and the structure and properties of KGM membrane were analyzed with Infrared spectrum, Raman spectrum, X-ray, SEM scanning and so on. The results revealed that the effects of different irradiation doses on the KGM molecular chain structure were different. Higher irradiation dose （20 kGy） resulted in partial damage against KGM membrane crystal structure, and there was no obvious change for the amorphous structure; with membrane property test, the tensile strength of KGM membrane gradually increased with the increase of irradiation dose and its elongation at break reduced, but these changes were not significant, WVP value reduced; with SEM, the membrane surface treated with irradiation was smoother even than the membrane without treatment. In addition, when increasing the irradiation dose, membrane surface became more even, and arrangement was more orderly and compact. KGM membrane nrooerties, and it is an ideal Irradiation modification could effectively improve the modification method.

关键词： KGM molecular chain irradiation modification membrane structure mechanicalproperty

Lipids and Fatty Acid Composition of Dried Edible Red and Black Ants

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Agricultural sciences in China 2010年第7期9卷 1072-1077页

作者： Oranut Sihamala Subhachai Bhulaidok department of food science and Nutrition Zhejiang University

Total lipid contents,lipid classes and fatty acid compositions of hot-air dried edible black ants（Polyrhachis vicina Roger） from Wenzhou and Guizhou,China,and edible red ant（Oecophylla smaragdina Fabricius） from Thailand were *** major lipid components were triacylglycerol（43.4-79.4% of total lipid）,followed by phospholipids（6.1-21.5%）,diacylglycerol（6.1-18.1%） and cholesterol ester（4.9-13.5%） while free fatty acids（1.8-2.9%） and sterol（0.5-0.8%） were the minor *** acid（C18：1） was the most predominant fatty acid,accounting for 3 407.8,9 098.8 and 3 790.1 mg 100 g-1 in Wenzhou and Guizhou black ants,and Thai red ant,*** by palmitic acid（C16：0）,922.9,2 523.3 and 1 511.8 mg 100 g-1;linoleic acid（C18：2n-6）,133.7,305.2 and 506.4 mg 100 g-1;α-linolenic acid（C18：3n-3）,35.1,138.0 and 52.5 mg 100 g-1;eicosapentaenoic acid（C20：5n-3） was 19.1,15.0 and 20.0 mg 100 g-1 in Wenzhou and Guizhou black ants,and Thai red ant,*** acid（C22：5n-3） was only found in black ants,7.0 and 7.3 mg 100 g-1 for Wenzhou and Guizhou ants,*** edible black and red ants were shown to be a good source of unsaturated fatty acid.

关键词： Polyrhachis vicina Roger Oecophylla smaragdina Fabricius edible black ant edible red ant polyunsaturated fatty acid (PUFA)