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Social skills and psychopathology are associated with autonomic function in children:a cross-sectional observational study

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Neural Regeneration Research 2022年第4期17卷 920-928页

作者： Elisa Cainelli Luca Vedovelli Daniele Bottigliengo Dario Boschiero Agnese Suppiej Child Neurology and Clinical Neurophysiology University Hospital of PadovaPadovaItaly department of General Psychology University of PadovaPadovaItaly Unit of Biostatistics Epidemiology and Public HealthDepartment of CardiacThoracicVascular Sciencesand Public HealthUniversity of PadovaPadovaItaly BioTekna biomedical Technologies Marcon(VE)Italy department of Medical sciences Pediatric SectionUniversity of FerraraFerraraItaly

In recent years,the increase of psychopathological disorders in the population has become a health emergency,leading to a great effort to understand psychological vulnerability mechanisms.In this scenario,the role of the autonomic nervous system(ANS)has become increasingly important.This study investigated the association between ANS,social skills,and psychopathological functioning in children.As an ANS status proxy,we measured heart rate variability(HRV).Infants admitted to the neonatal intensive care unit of the University Hospital of Padova because of preterm birth or neonatal hypoxic-ischemic encephalopathy were sequentially recruited from January 2011 to June 2013 and followed long-term up to school age in this cross-sectional observational study.We recorded 5 minutes of HRV immediately before measuring performance in social abilities tasks(affect recognition and theory of mind,NEPSY-II)in 50 children(mean age 7.4±1.4 years)with and without risk factors for developing neuropsychiatric disorders due to pre-/perinatal insults without major sequelae.Children also completed extensive cognitive,neuropsychological,and psychosocial assessment.Parents were assessed with psychopathological interviews and a questionnaire(CBCL 6-18).Analysis in a robust Bayesian framework was used to unearth dependencies between HRV,social skills,and psychopathological functioning.Social task scores were associated with HRV components,with high frequency the most consistent.HRV bands were also associated with the psychopathological questionnaire.Only normalized HRV high frequency was able to distinguish impaired children in the affect recognition task.Our data suggest that ANS may be implicated in social cognition both in typical and atypical developmental conditions and that HRV has cross-disease sensitivity.We suggest that HRV parameters may reflect a neurobiological vulnerability to psychopathology.The study was approved by the Ethics Committee of the University Hospital of Padova(Comitato Etico per la Sperimentazione,Azienda Opedal

关键词： affect recognition autonomic nervous system children hypoxic-ischemic encephalopathy impairment neonatal prematurity psychopathology recovery theory of mind

Mechanism of Learning and Memory Impairment in Rats Exposed to Arsenic and/or Fluoride Based on Microbiome and Metabolome

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biomedical and Environmental sciences 2023年第3期36卷 253-268页

作者： ZHANG Xiao Li YU Sheng Nan QU Ruo Di ZHAO Qiu Yi PAN Wei Zhe CHEN Xu Shen ZHANG Qian LIU Yan LI Jia GAO Yi LYU Yi YAN Xiao Yan LI Ben REN Xue Feng QIU Yu Lan department of Toxicology School of Public HealthShanxi Medical UniversityTaiyuan 030001ShanxiChina department of Microbiology Laboratory Linfen Central HospitalLinfen 041000ShanxiChina department of Epidemiology and Environmental health School of Public Health and Health ProfessionsUniversity at BuffaloBuffalo 14214New YorkUSA department of Pharmacology and Toxicology Jacobs School of Medicine and Biomedical SciencesUniversity at BuffaloBuffalo 1421New YorkUSA

Objective Arsenic(As) and fluoride(F) are two of the most common elements contaminating groundwater resources. A growing number of studies have found that As and F can cause neurotoxicity in infants and children, leading to cognitive, learning, and memory impairments. However, early biomarkers of learning and memory impairment induced by As and/or F remain unclear. In the present study, the mechanisms by which As and/or F cause learning memory impairment are explored at the multi-omics level(microbiome and metabolome).Methods We stablished an SD rats model exposed to arsenic and/or fluoride from intrauterine to adult period.Results Arsenic and/fluoride exposed groups showed reduced neurobehavioral performance and lesions in the hippocampal CA1 region. 16S rRNA gene sequencing revealed that As and/or F exposure significantly altered the composition and diversity of the gut microbiome, featuring the Lachnospiraceae_NK4A136_group, Ruminococcus_1, Prevotellaceae_NK3B31_group, [Eubacterium]_xylanophilum_group. Metabolome analysis showed that As and/or F-induced learning and memory impairment may be related to tryptophan, lipoic acid, glutamate, gamma-aminobutyric acidergic(GABAergic) synapse, and arachidonic acid(AA) metabolism. The gut microbiota, metabolites, and learning memory indicators were significantly correlated.Conclusion Learning memory impairment triggered by As and/or F exposure may be mediated by different gut microbes and their associated metabolites.

关键词： Arsenic Fluoride Learning and memory impairment Microbiome Metabolome

Anti-nitric oxide production, anti-proliferation and antioxidant effects of the aqueous extract from Tithonia diversifolia

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Asian Pacific Journal of Tropical Biomedicine 2016年第11期6卷 950-956页

作者： Poonsit Hiransai Jitbanjong Tangpong Chuthamat Kumbuar Namon Hoonheang Onrunee Rodpech Padchara Sangsuk Urairat Kajklangdon Waraphorn Inkaow Molecular Medicine and Cancer Biology Research Unit School of Allied Health Sciences and Public Health Walailak University department of Medical Technology School of Allied Health Sciences and Public Health Walailak University biomedical sciences Research Unit School of Allied Health Sciences and Public Health Walailak University

Objective: To determine the cytotoxicity, reduction in nitric oxide production and antioxidative activity of the aqueous leaf extract from Tithonia diversifolia(T. diversifolia) in an in vitro model.Methods: Leaves of T. diversifolia were collected from natural habitats and extracted with distilled water using the decoction method. The cytotoxic effect of the extract in terms of cell viability was determined using RAW264.7 cells and human peripheral blood mononuclear cells(PBMCs) via the mitochondrial respiration method using the MTT reagent. The effect of the extract on lipopolysaccharide(LPS)-induced nitric oxide production in RAW264.7 cells was measured using the Griess reagent. The chemical antioxidant was evaluated by ABTS- and DPPH-radical scavenging assays.Results: The half-maximal cytotoxic concentration values were 145.87 mg/m L and73.67 mg/m L for human PBMCs and RAW264.7 cells, respectively. In the presence of phytohemagglutinin-M, the IC_(50) on PBMCs proliferation was 4.42 mg/m L. The noncytotoxic range of the extracts inhibited LPS-induced nitrite production in RAW264.7 cells with an IC_(50) value of 11.63 mg/m L. To determine the anti-oxidative properties, the N-acetyl cysteine equivalent antioxidant capacity of the extract was(32.62 ± 1.87) and(20.99 ± 2.79)mg N-acetyl cysteine/g extract, respectively determined by the ABTS-radical and DPPHradical assay. However, the extract did not confer death protection in a hydrogen peroxideinduced RAW264.7 co-culturing model.Conclusions: Our study demonstrated the immunomodulation caused by the aqueous leaf extract of T. diversifolia, resulting from the inhibition of phytohemagglutinin-Minduced PBMCs proliferation and LPS-induced nitric oxide production in RAW264.7macrophages. Although the anti-oxidative activity was presented in the chemical-based anti-oxidant assay, the extract cannot protect cell death from stress conditions.

关键词： Tithonia diversifolia Anti-nitric oxide production Anti-proliferation Antioxidant

Large-scale analysis of the position-dependent binding and regulation of human RNA binding proteins

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Quantitative Biology 2020年第2期8卷 119-129页

作者： Jianan Lin Zhengqing Ouyang department of Biostatistics and Epidemiology School of Public Health and Health SciencesUniversity of MassachusettsAmherstMA 01003USA department of biomedical Engineering University of ConnecticutStorrsCT 06269USA

Background:RNA binding proteins(RBPs)play essential roles in the regulation of RNA metabolism.Recent studies have disclosed that RBPs achieve their functions via binding to their targets in a position-dependent pattern on RNAs.However,few studies have systematially addressed the associations between the RBP's functions and their positional binding preferences.Methods:Here,we present large-scale analyses on the functional targets of human RBPs by integrating the enhanced cross-linking and immunoprecipitation followed by sequencing(eCLIP-seq)datasets and the shRNA knockdown followed by RNA-seq datasets that are deposited in the integrated ENCyclopedia of DNA Elements in the human genome(ENCODE)data portal.Results:We found that(1)binding to the translation termination site and the 3'untranslated region is important to most human RBP's in the RNA decay regulation;(2)RBPs’binding and regulation follow a cell-ty pe specific pattern.Conclusions:These analysis results show the strong relationship between the binding position and the functions of RBPs,which provides novel insights into the RBPs'regulation mechanisms.

关键词： RNA binding protein CLIP-seq RNA-seq knockdown RNA regulation

Knowledge and associated factors of healthcare workers on measles vaccine and cold chain management at health institutions in Gondar,Ethiopia

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Asian Pacific Journal of Tropical Medicine 2023年第1期16卷 26-32页

作者： Aschalew Gelaw Yeshambel Belyhun Yitayih Wondimeneh Mehretie Kokeb Mulat Dagnew Azanaw Amare Mesert Mulu Martha Alemayehu Baye Gelaw department of Medical Microbiology School of Biomedical and Laboratory SciencesCollege of Medicine and Health SciencesUniversity of GondarGondarEthiopia department of Molecular Biology and Immunology School of Biomedical and Laboratory SciencesCollege of Medicine and Health Sciences University of GondarGondarEthiopia department of Pediatrics and Child health School of MedicineCollege of Medicine and Health SciencesUniversity of GondarGondarEthiopia

Objective:To assess the knowledge of healthcare workers on the measles vaccine and its cold chain management.Method:An institutional-based cross-sectional study was conducted from February 1 to March 30,2022 in Gondar City Administration public health institutions among 165 healthcare workers.Data were collected using a structured questionnaire.In addition,an on-spot observation checklist was used to assess the availability,status and management of the cold chain.A logistic regression model was used to assess the relationship between the outcome and predictor variables.Crude and adjusted odds ratios were calculated with 95%confidence intervals.Results:Overall,87(52.7%;95%CI 44.8%-60.5%)of the healthcare workers had unsatisfactory knowledge regarding the measles vaccine and its cold chain management.One hundred thirty-six(82.4%)healthcare workers correctly mentioned the recommended range of temperature(2-8℃)for measles vaccine storage.healthcare workers aged 18-29 years(P=0.001)and 30-44 years(P=0.014)were observed as determinants of unsatisfactory knowledge on the measles vaccine and its cold chain management.One hundred and five(63.6%)of the healthcare workers did not correctly mention the type of measles vaccine used in routine immunization.More than one-third(36.4%)of the healthcare workers perceived that the measles vaccine is not safe and could cause measles.Conclusions:More than half of the healthcare workers in the study area had unsatisfactory knowledge on the measles vaccine and its cold chain management.It is necessary to provide technical support and in-service training for healthcare workers to ensure optimal immunization effectiveness.

关键词： Measles vaccine healthcare workers Cold chain Gondar

Prevalence and Early Signs of Autism Spectrum Disorder(ASD) among 18-36 Month Old Children in Tianjin of China

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biomedical and Environmental sciences 2014年第6期27卷 453-461页

作者： HUANG Jun Ping CUI Shan Shan HAN Yu IRVA Hertz-Picciotto QI Li Hong ZHANG Xin School of public health Tianjin Medical University department of public health sciences University of California Davis

Objective The aim of this study is to estimate the prevalence of autism spectrum disorder(ASD) among 18-36 month old children in the Tianjin Municipality of China, and to identify early signs of autistic children and the predictability of each individual symptom. Methods A total of 8 000 children were screened to do a questionnaire based on CHAT modified to include more early signs of autism at the age of 18-36 months. Then the at-risk children were reexamined 1.5 years later and ASD children were identified based on DSM-IV. Early signs of autism were analyzed retrospectively by using discriminant function analysis performed among ASD children, children not followed up and children followed up but failing to meet ASD criteria. Results Three hundred and sixty seven children were screened as being at-risk to ASD, and 22 of them were identified as having ASD in the subsequent diagnosis. The prevalence of ASD was 27.5 per 10 000 in Tianjin of China with a male to female ratio of 4:1. Items addressing social interactions and communications had higher predictability than other items to distinguish autistic children from non-autistic ones. Pretend play, functional play, showing and reading parents' facial expressions distinguished autistic children from those not followed up, nevertheless those followed up but failing to meet ASD criteria were not included. Conclusion The prevalence of ASD found in our study was lower than that reported in some studies by western researchers. Autism has its specific symptoms, such as deficits in social awareness, social relatedness, and social referencing.

关键词： ASD 患病率天津市 6月龄自闭儿童早期中国

The Flavivirus Protease As a Target for Drug Discovery

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Virologica Sinica 2013年第6期28卷 326-336页

作者： Matthew Brecher Jing Zhang Hongmin Li Wadsworth Center New York State Department of Health department of biomedical sciences School of Public HealthState University of New York

Many flaviviruses are significant human pathogens causing considerable disease burdens,including encephalitis and hemorrhagic fever,in the regions in which they are endemic.A paucity of treatments for flaviviral infections has driven interest in drug development targeting proteins essential to flavivirus replication,such as the viral protease.During viral replication,the flavivirus genome is translated as a single polyprotein precursor,which must be cleaved into individual proteins by a complex of the viral protease,NS3,and its cofactor,NS2B.Because this cleavage is an obligate step of the viral life-cycle,the flavivirus protease is an attractive target for antiviral drug development.In this review,we will survey recent drug development studies targeting the NS3 active site,as well as studies targeting an NS2B/NS3interaction site determined from flavivirus protease crystal structures.

关键词：病毒感染蛋白酶药物发现药物开发病毒复制病毒基因组生活周期活性位点

Identification of BRAF V600E mutation in odontogenic tumors by high-performance MALDI-TOF analysis

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International Journal of Oral Science 2022年第2期14卷 212-219页

作者： Lucrezia Togni Antonio Zizzi Roberta Mazzucchelli Andrea Santarelli Corrado Rubini Marco Mascitti department of Clinical Specialistic and Dental sciences Marche Polytechnic Universityvia Tronto 10AnconaItaly Department of Biomedical Sciences and Public Health Marche Polytechnic Universityvia Tronto 10AnconaItaly Dentistry Clinic National Institute of Health and Science of AgingIRCCS INRCAvia Tronto 10AnconaItaly

Odontogenic tumors are rare lesions with unknown etiopathogenesis.Most of them are benign,but local aggressiveness,infiltrative potential,and high recurrence rate characterize some entities.The MAP-kinase pathway activation can represent a primary critical event in odontogenic tumorigenesis.Especially,the BRAF V600E mutation has been involved in 80%–90%of ameloblastic lesions,offering a biological rationale for developing new targeted therapies.The study aims to evaluate the BRAF V600E mutation in odontogenic lesions,comparing three different detection methods and focusing on the Sequenom MassARRAY System.81 surgical samples of odontogenic lesions were subjected to immunohistochemical analysis,Sanger Sequencing,and Matrix-Assisted Laser Desorption/Ionization-Time of Flight mass spectrometry(Sequenom).The BRAF V600E mutation was revealed only in ameloblastoma samples.Moreover,the presence of BRAF V600E was significantly associated with the mandibular site(ρ=0.627;P value<0.001)and the unicystic histotype(ρ=0.299,P value<0.001).However,any significant difference of 10-years disease-free survival time was not revealed.Finally,Sequenom showed to be a 100%sensitive and 98.1%specific,suggesting its high-performance diagnostic accuracy.These results suggest the MAP-kinase pathway could contribute to ameloblastic tumorigenesis.Moreover,they could indicate the anatomical specificity of the driving mutations of mandibular ameloblastomas,providing a biological rational for developing new targeted therapies.Finally,the high diagnostic accuracy of Sequenom was confirmed.

关键词： V600E BRAF specificity

NetBCE: An Interpretable Deep Neural Network for Accurate Prediction of Linear B-cell Epitopes

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Genomics, Proteomics & Bioinformatics 2022年第5期20卷 1002-1012页

作者： Haodong Xu Zhongming Zhao Center for Precision health School of Biomedical InformaticsThe University of Texas Health Science Center at HoustonHoustonTX 77030USA Human Genetics Center School of Public HealthThe University of Texas Health Science Center at HoustonHoustonTX 77030USA The University of Texas MD Anderson Cancer Center UThealth Houston Graduate School of biomedical sciences HoustonTX 77030USA department of biomedical Informatics Vanderbilt University Medical CenterNashvilleTN 37203USA

Identification of B-cell epitopes(BCEs)plays an essential role in the development of peptide vaccines and immuno-diagnostic reagents,as well as antibody design and production.In this work,we generated a large benchmark dataset comprising 124,879 experimentally supported linear epitope-containing regions in 3567 protein clusters from over 1.3 million B cell assays.Analysis of this curated dataset showed large pathogen diversity covering 176 different families.The accuracy in linear BCE prediction was found to strongly vary with different features,while all sequencederived and structural features were informative.To search more efficient and interpretive feature representations,a ten-layer deep learning framework for linear BCE prediction,namely Net BCE,was *** BCE achieved high accuracy and robust performance with the average area under the curve(AUC)value of 0.8455 in five-fold cross-validation through automatically learning the informative classification *** BCE substantially outperformed the conventional machine learning algorithms and other tools,with more than 22.06%improvement of AUC value compared to other tools using an independent dataset.Through investigating the output of important network modules in Net BCE,epitopes and non-epitopes tended to be presented in distinct regions with efficient feature representation along the network layer hierarchy.The Net BCE is freely available at https://***/bsml320/Net BCE.

关键词： prediction network value