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Chinese Science Bulletin 2002年第22期47卷 1866-1870页

作者： CHEN Zhongzhou TONG Yufeng CHEN Shuibing LI Yanmei CHEN YI ZHAO Yufen WANG Jinfeng Bioorganic Phosphorus Chemistry Laboratory Department of ChemistrySchool of Life Science and EngineeringTsinghua UniversityBeijing 100084China National Laboratory of Biomacromolecules Center for Molecular BiologyInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China

The peptide formation of N-phosphoryl aminoacids with amino acids proceeds in aqueous solution withoutany coupling reagents. After being separated in sephadex gelcolumn, the phosphoryl dipeptides were analyzed by theelectrospray ionization tandem mass spectrometry (ESIMS/MS). The result demonstrates that phosphoryl dipeptideswere datected in all the reaction systems. It is found tkat theformation of N-phosphoryl dipeptides is oriented: theN-terminal amino acid residues of the N-phosphoryl dipep-tides are from N-phosphoryl amino acids, and the peptideelongation happened at the C-terminal. Only adipeptide, noβ-dipeptide, is formed in the N-phosphoryl dipeptides,showing that α-carboxylic group is activated selectively byN-pbosphorylation. Theoretical calculation shows that thepeptide formation of N-phosphoryl amino acids might hap-pen through a pentu-coordinate carboxylic-phosphoric in-termediate in solution. These results might give some clues tothe stlidy on the origin of proteins and protein

关键词： N-phosphoryl amino acids orientation of peptide formation solution N-phosphoryl dipeptides.

Guest Editorial:Special Issue on Visualization and Computer Graphics

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Tsinghua Science and Technology 2012年第4期17卷 I0001-I0002页

作者： Jing Yang Shixia Liu Ye Zhao Ying-Qing Xu William Ribarsky department of Computer Science University of North Carolina at Charlotte Charlotte NC 28223 USA Internet Graphics group Microsoft Research Asia Beijing 100080 China department of Computer Science Kent State University Kent Ohio 44242 USA department of Information Art & Design Tsinghua University Beijing 100084 China

In the decade of data explosion, a significant challenge is how to transform data into understandings and insights that are useful to people. Visualization, the use of computer-supported, interactive visual representations of data to amplify cognition, is an important approach to addressing this challenge. In particular, scientific visualization primarily represents physical or geometric data while information visualization mainly represents abstract data such as text documents, graphs, and multidimensional data. Visual analytics, springing out of the fields of information visualization and scientific visualization,

关键词： Special issues and sections Visual analytics Algorithm design and analysis Data visualization

Sebaceous gland cells differentiation of hair follicle bulge cells induced by peroxisome proliferator activation receptor γ2 expression

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Sebaceous gland cells differentiation of hair follicle bulge...

Early versus delayed necrosectomy in pancreatic necrosis:A population-based cohort study on readmission,healthcare utilization,and in-hospital mortality

中国细胞生物学学会2005年学术大会、青年学术研讨会

作者：符刚高强国杨恬 department of Cell biology Third Military Medical University Chongqing 400038 department of Cell biology Third Military Medical University Chongqing 400038 department of Cell biology Third Military Medical University Chongqing 400038

To study the mechanism of differntiation of hair follcle bulge cells into sebaceous gland cells. We construct peroxisome proliferator activation receptor γ 2 (PPAR γ 2)expression plasmid of pEGFPC1-uPAR gene, then transfected the plasmids into the hair follicle bulge cells mediated by liposome, another two groups of hair follicle bulge cells: hair follicle bulge cells and those transfected with blank pEGFPC1 plasmids were used as controls. The morphological change of the hair follicle bulge cells were observed, and immunocytochemical staining were used to detect expressing of EMA hair follicle bulge cells and oil red "O" staining were used to observed the lipid droplet synthesis of *** which transfected the plasmids expressed bright-green fluorescence. The experiment group cells can expressed PPAγR2 gene, after 10 days postdifferentiation lipid droplet were observed in cytoplasm were observed with oil red "O" staining and cells positive expressed EMA, but those phenomenon not occurrence two control *** data show that the hair follicle bulge cells have the capability to differentiation into sebaceous gland cells in vitro, and PPAγR2 play an important role in differentiation.

关键词： EMA Sebaceous gland cells differentiation of hair follicle bulge cells induced by peroxisome proliferator activation receptor expression red

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World Journal of Methodology 2024年第3期14卷 55-67页

作者： Hassam Ali Faisal Inayat Vinay Jahagirdar Fouad Jaber Arslan Afzal Pratik Patel Hamza Tahir Muhammad Sajeel Anwar Attiq Ur Rehman Muhammad Sarfraz Ahtshamullah Chaudhry Gul Nawaz Dushyant Singh Dahiya Amir H Sohail Muhammad Aziz Division of Gastroenterology and Hepatology East Carolina University Brody School of MedicineGreenvilleNC 27834United States department of Internal Medicine Allama Iqbal Medical CollegeLahorePunjab 54550Pakistan department of Internal Medicine University of Missouri-Kansas CityKansas CityMO 64108United States Division of Gastroenterology Mather Hospital and Zucker School of Medicine at Hofstra UniversityPort JeffersonNY 11777United States department of Internal Medicine Jefferson Einstein HospitalPhiladelphiaPA 19141United States department of Internal Medicine UHS Wilson Medical CenterJohnson CityNY 13790United States Division of Gastroenterology and Hepatology Geisinger Wyoming Valley Medical CenterWilkes-BarrePA 18711United States department of Internal Medicine St.Dominic's HospitalJacksonMS 39216United States Division of Gastroenterology Hepatologyand MotilityThe University of Kansas School of MedicineKansas CityKS 66160United States department of Surgery University of New Mexico School of MedicineAlbuquerqueNM 87106United States Division of Gastroenterology and Hepatology The University of ToledoToledoOH 43606United States

BACKGROUND Acute necrotizing pancreatitis is a severe and life-threatening *** poses a considerable challenge for clinicians due to its complex nature and the high risk of *** minimally invasive and open necrosectomy procedures have been *** advancements in treatment modalities,the optimal timing to perform necrosectomy lacks *** To evaluate the impact of necrosectomy timing on patients with pancreatic necrosis in the United *** A national retrospective cohort study was conducted using the 2016-2019 Nationwide Readmissions *** with non-elective admissions for pancreatic necrosis were *** participants were divided into two groups based on the necrosectomy timing:The early group received intervention within 48 hours,whereas the delayed group underwent the procedure after 48 *** various intervention techniques included endoscopic,percutaneous,or surgical *** major outcomes of interest were 30-day readmission rates,healthcare utilization,and inpatient *** A total of 1309 patients with pancreatic necrosis were *** propensity score matching,349 cases treated with early necrosectomy were matched to 375 controls who received delayed *** early cohort had a 30-day readmission rate of 8.6% compared to 4.8%in the delayed cohort(P=0.040).Early necrosectomy had lower rates of mechanical ventilation(2.9%vs 10.9%,Pgroup incurred lower healthcare costs,with median total charges of $52202 compared to$147418 in the delayed *** in the early cohort also had a relatively shorter median length of stay(6 vs 16 days,P<0.001).The timing of necrosectomy did not significantly influence the risk of 30-day readmission,with a hazard ratio of 0.56(95%confidence interval:0.31-1.02,P=0.06).CONCLUSION Our findings show that

关键词： Acute necrotizing pancreatitis Pancreatic necrosis Early necrosectomy Delayed necrosectomy Readmission,Healthcare costs Mortality

Sirtuin 1 in rat orthotopic liver transplantation:An I GL-1 preservation solution approach

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World Journal of Gastroenterology 2015年第6期21卷 1765-1774页

作者： Eirini Pantazi Mohamed Amine Zaouali Mohamed Bejaoui Emma Folch-Puy Hassen Ben Abdennebi Ana Teresa Varela Anabela Pinto Rolo Carlos Marques Palmeira Joan Roselló-Catafau Experimental Hepatic Ischemia Reperfusion Unit Institute of Biomedical Research of Barcelona Faculty of Pharmacy Molecular Biology and Anthropology Applied to Development and Health department of Life Sciences and Center for Neuroscience and Cell biology University of Coimbra department of biology University of Aveiro

AIM:To investigate the possible involvement of Sirtuin1(SIRT1)in rat orthotopic liver transplantation(OLT),when Institute Georges Lopez 1(IGL-1)preservation solution is enriched with trimetazidine(TMZ).METHODS:Male Sprague-Dawley rats were used as donors and *** were stored in IGL-1 preservation solution for 8h at 4℃,and then underwent OLT according to Kamada’s cuff technique without *** another group,livers were stored in IGL-1 preservation solution supplemented with TMZ,at10-6 mol/L,for 8 h at 4℃and then underwent *** were sacrificed 24 h after reperfusion,and liver and plasma samples were *** injury(transaminase levels),mitochondrial damage(glutamate dehydrogenase activity)oxidative stress(malondialdehyde levels),and nicotinamide adenine dinucleotide(NAD+),the cofactor necessary for SIRT1 activity,were determined by biochemical ***1 and its substrates(acFox O1,ac-p53),the precursor of NAD+,nicotinamide phosphoribosyltransferase(NAMPT),as well as the phosphorylation of adenosine monophosphate activated protein kinase(AMPK),p-m TOR,p-p70S6K(direct substrate of m TOR),autophagy parameters(beclin-1,LC3B)and MAP kinases(p-p38 and p-ERK)were determined by Western ***:Liver grafts preserved in IGL-1 solution enriched with TMZ presented reduced liver injury and mitochondrial damage compared with those preservedin IGL-1 solution *** addition,livers preserved in IGL-1+TMZ presented reduced levels of oxidative *** was consistent with enhanced SIRT1 protein expression and elevated SIRT1 activity,as indicated by decreased acetylation of p53 and Fox *** elevated SIRT1 activity in presence of TMZ can be attributed to the enhanced NAMPT protein and NAD+/NADH ***-regulation of SIRT1 was consistent with activation of AMPK and inhibition of phosphorylation of m TOR and its direct substrate(p-p70S6K).As a consequence,autophagy mediators(beclin-1 and LC3B)were ***,MAP kinases w

关键词： Sirtuin 1 Ischemia-reperfusion injury Liver transp

Mutational Analysis of OCT4+ and OCT4− Circulating Tumour Cells by Single Cell Whole Exome Sequencing in Stage I Non-Small Cell Lung Cancer Patients

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Journal of Shanghai Jiaotong university(Science) 2021年第1期26卷 40-46页

作者： YAN Bo FU Shijie CHANG Yuanyuan GU Aiqin DONG Qianggang LI Rong department of Pulmonary Medicine Shanghai Chest HospitalShanghai Jiao Tong UniversityShanghai 200030China department of Thoracic Surgery Shanghai Chest HospitalShanghai Jiao Tong UniversityShanghai 200030China Cancer Stem Cell Research group Shanghai Jiao Tong University Cancer InstituteShanghai 200032China

Circulating tumour cells(CTCs)were enriched in the peripheral blood of four patients with Stage I non-small cell lung cancer(NSCLC).Octamer-binding transcription factor-4 positive(OCT4+)and negative(OCT4−)CTCs were identified and captured by interphase fluorescence in situ hybridisation(iFISH).Single cell whole exome sequencing(WES)was performed and the corresponding bioinformatics data were ***4+cells were successfully detected in peripheral blood collected from all four Stage I lung cancer ***,the tumour mutational burden(TMB)values observed for OCT4+samples from the same patients were slightly smaller than those of the OCT4−samples;the difference was not statistically significant(P>0.05).Thirteen and six characteristic mutations were found in negative samples and positive samples,*** findings indicate that this methodology provides a potential diagnostic index for the early detection of NSCLC.

关键词： non-small cell lung cancer(NSCLC) octamer-binding transcription factor-4(OCT4) circulating tumour cells(CTCs) whole exome sequencing(WES)

Neurocognitive outcomes in pediatric brain tumors after treatment with proton versus photon radiation:a systematic review and meta-analysis

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世界儿科杂志:英文版 2023年第8期19卷 727-740页

作者： Álvaro Lassaletta Javier S.Morales Pedro L.Valenzuela Borja Esteso Lisa S.Kahalley Donald J.Mabbott Soumya Unnikrishnan Elena Panizo Felipe Calvo Radiation Oncology department Clínica Universidad de NavarraCalle Marquesado de Santa Marta 128027 MadridSpain Pediatric Neuro-Oncology Unit Hospital Infantil Universitario Niño JesúsMadridSpain MOVE-IT Research group Department of Physical EducationFaculty of Education SciencesUniversity of CadizCádizSpain Biomedical Research and Innovation Institute of Cádiz(INiBICA)Research Unit Puerta del Mar University HospitalUniversity of CádizCádizSpain Physical Activity and Health Research group(PaHerg) Research Institute of the Hospital 12 de Octubre(‘imas12’)MadridSpain Systems biology department University of AlcaláMadridSpain Clinical Neuropsychology Unit Psychiatry and Clinical Psychology DepartmentHospital Infantil Universitario Niño JesúsMadridSpain Baylor College of Medicine HoustonTXUSA Texas Children’s Hospital HoustonTXUSA The Hospital for Sick Children TorontoONCanada The University of Toronto TorontoONCanada University of California School of Medicine San DiegoLa JollaCAUSA

Background Advances in cancer treatments,particularly the development of radiation therapy,have led to improvements in survival outcomes in children with brain ***,radiation therapy is associated with significant long-term neurocognitive *** present systematic review and meta-analysis aimed to compare the neurocognitive outcomes of children and adolescents with brain tumors treated with photon radiation(XRT)or proton therapy(PBRT).Methods A systematic search was conducted(PubMed,Embase,Cochrane,and Web of Science from inception until 02/01/2022)for studies comparing the neurocognitive outcomes of children and adolescents with brain tumors treated with XRT *** pooled mean differences(expressed as Z scores)were calculated using a random effects method for those endpoints analyzed by a minimum of three *** Totally 10 studies(n=630 patients,average age range:1–20 years)met the inclusion *** who had received PBRT achieved significantly higher scores(difference in Z scores ranging from 0.29–0.75,all P0.05 in main analyses or sensitivity analyses)were found for nonverbal memory,verbal working memory and working memory index,processing speed index,or focused *** Pediatric brain tumor patients who receive PBRT achieve significantly higher scores on most neurocognitive outcomes than those who receive *** studies with long-term follow-ups are needed to confirm these results.

关键词： Brain health Childhood cancer Intelligence quotient Radiotherapy

A comparative in vitro study on the cerumenolytic effect of docusate sodium versus 2.5%sodium bicarbonate using UVevisible absorption spectroscopy

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Journal of Otology 2020年第3期15卷 99-102页

作者： Chompunut Srisukhumchai Pornthep Kasemsiri Teeraporn Rattanaanekchai Somchai Srirompotong Umaporn Yordpratum Chadamas Sakolsinsiri department of Otorhinolaryngology Srinagarind HospitalFaculty of Medicine at the Khon Kaen UniversityKhon KaenThailand Khon Kaen Ear Hearing and Balance Research group Khon Kaen UniversityThailand department of Microbiology Faculty of MedicineKhon Kaen UniversityThailand department of Biochemistry Faculty of MedicineKhon Kaen UniversityThailand

Objective:To compare cerumenolytic effects of docusate sodium and of 2.5%sodium bicarbonate-In vitro study;observe characteristics of the solution,using ultravioletevisible(UV/Vis)spectroscopy,and measurement of cholesterol ***:Samples of human cerumen were mixed to form a relatively homogenous *** of about 500mg were weighed and packed at the bottom of the test *** each tube was added 1.5 ml of either docusate sodium or 2.5%sodium *** were incubated at 36.4C in a water bath for 15,30 or 60 *** incubation,the supernatant solution was pipetted into a *** cerumenolytic efficacy was defined as the absorbance(recorded at 350 nm and 400 nm)of the *** were the average of three replicates.A cholesterol level of each sample was then determined to confirm the ***:Turbidity was much greater in tubes containing 2.5%sodium bicarbonate,indicating dissolution of *** difference of absorbance values measured at 350 nm and 400 nm after 15,30,60 min digestions were 1.93[95%CI 1.49e2.38,p-value<0.001]and 1.81[95%CI 1.21e2.41,p-value<0.001],***,levels of cholesterol were greater in tubes containing 2.5%sodium bicarbonate solution after digestion than in tubes containing docusate sodium;11 mg/dl[95%CI 1.47e24.14,pvalue?0.083]Conclusion:Both spectrophotometric and cholesterol level assessments suggest that 2.5%sodium bicarbonate has a higher cerumenolytic effect than docusate *** other words,cerumen can be dissolved in 2.5%sodium bicarbonate much better than docusate sodium in a time-dependent manner.

关键词： Cerumen Cerumenolytic agents Sodium bicarbonate Docusate sodium

Sodium and health: New proposal of distribution for major meals

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Health 2014年第3期6卷 195-201页

作者： Renata Puppin Zandonadi Raquel BABotelho Veronica CGinani Rita de Cassia CAAkutsu Karin Eleonora de Oliveira Savio Wilma MCAraujo Research group in Nutritional and Nourishment Quality Department of NutritionUniversity of BrasiliaBrasiliaBrazil

Sodium ingestion in regular diet exceeds individuals’ physiological needs, and it is etiologically related to various diseases. Studies estimate that the worldwide average individual intake of sodium reaches almost double the maximum limit of ingestion for the day only in the main meals: lunch and dinner. Culturally, sodium is highly used in meals to enhance the flavor of preparations. Then, the purposes of this research are to evaluate sodium use in meals prepared in Popular Brazilian Restaurants and to suggest the distribution of sodium for each kind of preparation of major meals. This is an exploratory cross-sectional research based on direct documentation subdivided in six steps: determination of samples of foodservices and menus;development of the technical preparation files offered in the menus;determination of the weight of average portions offered;determination of the average amount of sodium in the portions offered;estimation of sodium distribution regarding two parameters;and determination of the sodium distribution among preparations of major meals by the two proposed parameters. For results’ analysis, portions were calculated as well as the mean contribution of the preparation in the plate weight in percentage and the mean sodium contribution. Standard deviation is also presented. We verified through the study the possibility of using the distribution of sodium by two parameters: the first one considers the ratio of components’ weight in the menu in relation to the total weight of the meal;the second one considers the distribution of sodium in preparations in relation to the total amount of sodium in the meal. We expect the results of this research to improve menu planning in restaurants as to fulfill the actual clients’ needs in order to prevent diseases.

关键词： Sodium Menu Foodservice Parameter Meal Lunch