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Suppression of Sup35 amyloid fibril formation by group II chaperonin from <i>Thermoplasma acidophilum</i>

American Journal of Molecular Biology 2012年第3期2卷 265-275页

作者： Kentaro Noi Aya Kitamura Hidenori Hirai Kunihiro Hongo Toshihiko Sakurai Tomohiro Mizobata Yasushi Kawata department of Biomedical Science Institute of Regenerative Medicine and Biofunction Graduate School of Medical Science department of Chemistry and Biotechnology Graduate School of Engineering Tottori University Tottori Japan

The Group II chaperonin from Thermoplasma acidophilum was added to the in vitro amyloid fibrillation reaction of yeast Sup35NM protein to assess its effects. By measuring the formation of Sup35NM fibrils in real time using the fluorescent dye Thioflavin T, we found that the addition of T. acidophilum-cpn α16, α1, and β1 proteins suppressed fibril formation. Addition of a 0.1 molar-equivalent T. acidophilum-cpn α16 relative to Sup35NM prolonged the initial lag-time of fibril formation and decreased the rate of fibril extension. Addition of 1 or 3 molar-equivalents of T. acidophilum-cpn monomers also produced a similar effect. Delayed addition of these chaperonins after the initial lag phase did not suppress fibril formation. Interestingly, these effects were also observed upon adding only the apical domain segments of α and β-subunits, and we also found that deletion of the helical protrusion in the apical domain of these segments led to an abolishment of the suppression effects. A synthetic peptide whose sequence corresponded to the helical protrusion also displayed a suppression effect, which indicated that archaeal group II chaperonin binds to Sup35NM through the helical protrusion of the apical domain. These findings suggest that group II chaperonin might be actively involved in suppressing amyloid fibril formation, in addition to acting as a protein folding assistant.

关键词： Group II Chaperonin Monomer Thermoplasma Acidophilum Structure and Function Suppression of Amyloid Fibril Sup35NM Amyloid

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A Novel Ratiometric Fluorescent Probe for Cyanide Anion with High Selectivity and Its Application in Cell Imaging

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Chemical Research in Chinese Universities 2017年第4期33卷 534-539页

作者： FENG Yusha BAI Lifei WANG Shaodan KONG Xianyi CONG Lele ZHAO Qing YANG Qingbiao LIYaoxian department of Neurology China-Japan Union Hospital of Jilin University Changehun 130031 P. R. ChinaI College of Chemistry Jilin University Changchun 130021 China Jiangsu Key Laboratory of biofunction Molecule College of Life Science and Cheimistry & Chemical Engineering Jiangsu Second Normal University Nanjing 210013 P. R. China

A novel hybrid acenaphthene-hemicyanine dye（Acc） was designed and synthesized as a colorimetric and ratiometric fluorescent sensor for cyanide detection. The nucleophilic addition of cyanide anion resulted in the blocking of the intramolecular charge transfer（ICT）. Thus, the sensor exhibited high sensitivity and selectivity to- wards cyanide anion among many anions in dimethylsulphoxide（DMSO）-water media, with the emission wavelength displaying a very large shift（186 nm）. Moreover, the color changes of the solution could be observed by the naked eyes and it had a short response time. Under optimized conditions, the detection limit for cyanide was calculated to be 1.3μmol/L（R2=0.9958）. In addition, the probe had excellent biocompatibility and could be utilized to monitor cyanide in living cells.

关键词： Ratiometric fluorescence Cyanide Nucleophilic addition Bioimaging

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Low-Flow Polysulfone Hemodialysis Alters Lipoprotein Parameters, Paraoxonase Activity and <i>in Vitro</i>Incorporation of Phospholipids

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Journal of Biophysical Chemistry 2017年第3期8卷 23-38页

作者： Franciane S. Marques Alysson Luiz M. Da Silva Ricardo J. C. Mattoso Roque Aras Rogério Jorge B. De Oliveira Júlio Cézar De Abreu Santos Fabio D. Couto Ricardo D. Couto PGBSMI/Goncalo Moniz Research Center (CPqGM) FIOCRUZ Foundation Center Salvador Brazil department of biofunction Health Institute of Science Federal University of Bahia/UFBA Salvador Brazil Clinical Biochemistry Laboratory Department of Clinical and Toxicological Analysis Faculty of Pharmacy Federal University of Bahia/UFBA Salvador Brazil Ana Neri Hospital SESAB and Federal University of Bahia/UFBA Salvador Brazil Naval Hospital of Salvador Brazilian Marine Forces Salvador Brazil Laboratory of Molecular Biology and Genetics at Center of Agricultural Environmental and Biological Sciences (CCAAB-UFRB) Cruz das Almas Brazil

End stage-renal-disease (ESRD) is associated with dyslipidemia and premature atherosclerosis. The study evaluates the effect of hemodialysis (HD) on HDL-remodeling between pre- and post-HD. Was conducted a cross-sectional study with 50 ESRD male patients, undergoing HD at Ana Neri Hospital, Salvador, Brazil. All individuals were on HD for at least 3 months, into a three sessions protocol for 3 - 4 hours per week, with a polysulfone low-flow basic-dialyzing-membrane and unfractionated-heparin. HDL Phospholipid-incorporation was measured by 14C-PL-scintillation-counting, expressed as %14C-PL/mL/hour. Paraoxonase (PON-1) activity was measured by spectrophotometry using paraoxon as substrate. Cardiovascular risk ratios and atherogenic index of plasma were calculated. Total cholesterol, HDL-C and non-HDL-C increased at post-HD on all age groups, but without triglycerides (TG) changes. TG/HDL-C decreased in 30 - 39 and 40 - 49 year (y) at post-HD (p 0.05). LDL-C/apoB increased in >60 y, after HD (p 60 (p 0.01). On the other hand, non-HDL-C/HDL-C reduced in 40 - 49 and >60 y, at post-HD (p 0.05). The linear-correlation between %14C-PL-incorporation and non-HDL-C/HDL-C was negative in 30 - 39 y, both at pre-HD (r = -0.90;p = 0.002) and post-HD (r = -0.78;p = 0.022). Linear-correlation between PON-1 and %14C-PL-incorporation was positive in >60 y, both at pre- (r = 0.63;p = 0.029) and post-HD (r = 0.65;p = 0.022). PON-1 activity increased at pre- (59 ± 30) and post-HD (73 ± 38) in 50 - 59 y (p 0.05). The %14C-PL-incorporation was reduced in >60 y (p 0.05), when compared to pre- and post-HD. ESRD patients undergoing HD shows important changes on lipid-profile, PON-1-activity, cardiac risk ratios and HDL-remodeling. These results demonstrate the influence of HD with a polysulfone low-flow basic-dialyzing-membrane and unfractionated-heparin on lipoprotein metabolism.

关键词： Hemodialysis Dyslipidemia Cardiovascular Risk Bioindices PLTP PON-1 HDL-Remodeling Low-Flow Polysulfone Dialyzing-Membrane

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Identification and verification of aging-related lncRNAs for prognosis prediction and immune microenvironment in patients with head and neck squamous carcinoma

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Oncology Research 2023年第1期31卷 35-61页

作者： QING GAO YUJING SHI YUANYUAN SUN SHU ZHOU ZEYUAN LIU XINCHEN SUN XIAOKE DI department of Radiation Oncology The First Affiliated Hospital of Nanjing Medical UniversityNanjing210029China department of Oncology Jurong People’s HospitalJurong212499China department of Radiation Oncology Nanjing Jiangning Hospital and the Affiliated Jiangning Hospital of Nanjing Medical UniversityNanjing211199China

Aging is highly associated with tumor formation and ***,little research has explored the association of aging-related lncRNAs(ARLs)with the prognosis and tumor immune microenvironment(TIME)of head and neck squamous cell carcinoma(HNSCC).RNA sequences and clinicopathological data of HNSCC patients and normal subjects were downloaded from The Cancer Genome *** the training group,we used Pearson correlation,univariate Cox regression,least absolute shrinkage/selection operator regression analyses,and multivariate Cox regression to build a prognostic *** the test group,we evaluated the *** Cox regression was done to screen out independent prognostic factors,with which we constructed a ***,we demonstrated the predictive value of the risk scores based on the model and the nomogram using time-dependent receiver operating *** set enrichment analysis,immune correlation analysis,and half-maximal inhibitory concentration were also performed to reveal the different landscapes of TIME between risk groups and to predict immuno-and chemo-therapeutic *** most important LINC00861 in the model was examined in HNE1,CNE1,and CNE2 nasopharyngeal carcinoma cell lines and transfected into the cell lines CNE1 and CNE2 using the LINC00861-pcDNA3.1 construct *** addition,CCK-8,Edu,and SA-β-gal staining assays were conducted to test the biofunction of LINC00861 in the CNE1 and CNE2 *** signature based on nine ARLs has a good predictive value in survival time,immune infiltration,immune checkpoint expression,and sensitivity to multiple ***00861 expression in CNE2 was significantly lower than in the HNE1 and CNE1 cells,and LINC00861 overexpression significantly inhibited the proliferation and increased the senescence of nasopharyngeal carcinoma cell *** work built and verified a new prognostic model for HNSCC based on ARLs and mapped the immune landscape in ***00861 is a protective

关键词： Aging lncRNA HNSCC Prognosis Tumor immune microenvironment Bioinformatics

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Synaptophysin-like 2 expression correlates with lymph node metastasis and poor prognosis in colorectal cancer patients

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World Journal of Gastrointestinal Oncology 2022年第11期14卷 2122-2137页

作者： Zong-Xian Zhao Qin-Lingfei Liu Yao Yuan Fu-Sheng Wang department of Anorectal Surgery Fuyang People’s HospitalFuyang 236000Anhui ProvinceChina department of Digestive Internal Medicine Tianjin Medical University General HospitalTianjin 300070China

BACKGROUND Colorectal cancer(CRC)is one of the most common and fatal cancers ***-like 2(SYPL2)is a neuroendocrine-related protein highly expressed in skeletal muscle and the *** involvement of SYPL2 in CRC,including its level of expression and function,has not been *** To evaluate the correlations of SYPL2 expression with lymph node metastasis(LNM)and prognosis in patients with *** The levels of expression of SYPL2 in CRC and normal colorectal tissues were analyzed in multiple public and online *** associations between clinical variables and SYPL2 expression were evaluated statistically,and the associations between SYPL2 expression and prognosis in patients with CRC were analyzed using the Kaplan-Meier method and univariate/multivariate Cox regression ***2 expression was assessed in 20 paired CRC tissue and adjacent normal colorectal tissue samples obtained from Fuyang People’s Hospital,and the associations between SYPL2 expression and the clinical characteristics of these patients were *** between the levels of expression of SYPL2 and key targeted genes were determined by Pearson’s correlation *** distribution of immune cells in these samples was calculated using the CIBERSORT *** set enrichment analysis(GSEA)was performed to evaluate the biofunction and pathways of SYPL2 in *** SYPL2 expression was significantly lower in CRC tissue samples than in normal colorectal tissue samples(P<0.05).High SYPL2 levels in CRC tissues correlated significantly with LNM(P<0.05)and a poorer patient prognosis,including significantly shorter overall survival(OS)[hazard ratio(HR)=1.9,P<0.05]and disease-free survival(HR=1.6,P<0.05).High SYPL2 expression was an independent risk factor for OS in both univariate(HR=2.078,P=0.014)and multivariate(HR=1.754,P=0.018)Cox regression *** addition,SYPL2 expression correlated significantly with the expression of KDR(P<0.0001,r

关键词： Synaptophysin-like 2 Colorectal cancer Lymph node metastasis Prognosis Immune microenvironment Bevacizumab

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Bioinformatics Based Therapeutic Effects of Sinomenium Acutum

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Chinese Journal of Integrative Medicine 2019年第2期25卷 122-130页

作者： LI Yu-yan ZHENG Guang LIU Liang Faculty of Chinese Medicine Macao University of Science and Technology department of Nephrology Shenzhen Traditional Chinese Medicine Hospital School of Information Science and Engineering Lanzhou University

Objective: To decipher the possible mechanisms of Sinomenium Acutum(SA) in treating diseases by a bioinformatics method. Methods: SA ingredients were searched according to Chinese Pharmacopoeia, Chinese Medicine Dictionary and Traditional Chinese Medicines Database(TCMD). Active compounds and target proteins of SA were acquired through the Pubchem platform. Pathway, network and function analyses of SA were performed with ingenuity pathway analysis(IPA), a bioinformatics analysis platform. Disease, biofunction-target networks were established with Cytoscape. Results: Eighteen ingredients from SA were obtained. Seven active ingredients with 31 active target proteins were acquired according to PubChem Bioassay test. By IPA analysis, 277 canonical pathways belonging to 17 function categories were collected, 23 kinds of diseases, 21 categories bio-functions were obtained. Based on P value, calculated by IPA, the top 5 significant pathway of SA targets include phosphatidylinositol 3 kinase/Akt(PI3 K/Akt) signaling, prostate cancer signaling, macrophage migration inhibitory factor(MIF) regulation of innate immunity, Guanosine-binding protein coupled receptor(GPCR) signaling, and ataxia telangiectasia mutated protein(ATM) signaling. Disease and bio-function network analysis indicated that mitogen activated protein kinase 1(MAPK1), MAPK3, p65 nuclear factor κB(RELA), nuclear factor of κB inhibitor alpha(NFκBIA), interleukin 1β(IL-1β), prostaglandin G/H synthase 2(PTGS2) and tumor protein 53(TP53) were the critical targets in various diseases treated by SA. Conclusions: In the different view of target, pathway, disease and bio-function, inflammation was found to be a central theme in many chronic conditions. SA could be used not only as an anti-inflammatory agent, but also for the treatment of cancers, neurological diseases, psychological disorders and metabolic diseases.

关键词： Sinomenium Acutum bioinformatics analysis ingenuity pathway analysis network pharmacology

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Renal Effects of Prosthetic Abdominal Aortic Aneurysm in Dogs

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畜牧与兽医 2012年第S2期44卷 44-45页

作者： LV Yu-huan XU Zai-pin XU Wanli MA Zhe PENG Yao DU Peihang SUN Anqiang Deng Xiaoyan LOU Hanjun department of Animal Medicine Guizhou University College of Biological & Medical Engineering Beihang University

To provide experimental model for abdominal aortic aneurysm research and to optimize design of stent-graft,the authors established an animal models of prosthetic abdominal aortic aneurysm (PAAA) in dogs,evaluated its biofunction,biocompatibility and renal *** PAAA was sutured using aneurysm neck (Φ 6 mm artificial blood vessel) and aneurysm body (Φ 29 and 31mm artificial blood vessel,respectively). Sixteen healthy adult dogs,with weight of 13. 5 ± 0. 66 kg,were randomly divided into 2 groups: group 29 mm and group 31 mm,8 in each group. Infrarenal aortic artery was reconstructed with PAAA to establish abdominal aortic aneurysm (AAA) animal model. Blood coagulation index such as prothrombin time (PT),activated partial thromboplatin time (APTT),thrombin time (TT) and Fibrinogen (Fbg) were detected pre-operation,1,7 and 14 days post-operation,respectively. Blood routine parameters were detected pre-operation 11,3 and 7 days post-operation. Renal function parameters such as blood urea nitrogen (BUN),creatinine (CREA),urea acid (UA) and urea/ creatinine (BU/CREA) were evaluated pre-operation and 30,90,150 days post-operation,respectively. PAAA were removed en bloc to determinine its biofuction and biocompatibility after 5 months of surgery. Kidneys were fixed in neutral buffered formalin solution,pathological slice were conducted to observate pathological changes of kidneys by HE *** results are as follows:1. Animal model: The PAAA was implanted to the infrarenal abdominal aortic artery without extensive bleeding after anastomosis. One dog died 2,25 and 127 days post-operation in group 31 mm,respectively,and the overall success rate was 62. 5%. One dog died 7 days post-operation in group 29 mm,the overall success rate was 87. 5%.2. biofunction and biocompatibility of PAAA: Autopsy showed that there were no abdominal adhesions around the PAAAs,the lumen was patent without blood clots,there were no bleeding in PAAAs in two groups. Biocompatibility was poor bec

关键词： Abdominal aortic aneurysm Animal model Artificial blood vessel Canine

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biofunctional magnesium coating of implant materials by physical vapour deposition

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Biomaterials Translational 2021年第3期2卷 248-256页

作者： Qingchuan Wang Weidan Wang Yanfang Li Weirong Li Lili Tan Ke Yang department of Advanced Materials Research Institute of Metal ResearchChinese Academy of SciencesShenyangLiaoning ProvinceChina department of Orthopaedic Surgery Affiliated Zhongshan Hospital of Dalian UniversityDalianLiaoning ProvinceChina Dongguan Eontec Co.Ltd. DongguanGuangdong ProvinceChina

The lack of bioactivity of conventional medical materials leads to low osseointegration ability that may result in the occurrence of aseptic loosening in the *** achieve high osseointegration,surface modifications with multiple biofunctions including degradability,osteogenesis,angiogenesis and antibacterial properties are ***,the functions of conventional bioactive coatings are *** novel biofunctional magnesium(Mg)coatings are believed to be promising candidates for surface modification of implant materials for use in bone tissue *** physical vapour deposition,many previous researchers have deposited Mg coatings with high purity and granular microstructure on titanium alloys,polyetheretherketone,steels,Mg alloys and *** was found that the Mg coatings with high-purity could considerably control the degradation rate in the initial stage of Mg alloy implantation,which is the most important problem for the application of Mg alloy *** addition,Mg coating on titanium(Ti)implant materials has been extensively studied both in vitro and in vivo,and the results indicated that their corrosion behaviour and biocompatibility are *** coatings continuously release Mg ions during the degradation process,and the alkaline environment caused by Mg degradation has obvious antibacterial ***,the Mg coating has beneficial effects on osteogenesis and osseointegration,and increases the new bone-regenerating *** coatings also exhibit favourable osteogenic and angiogenic properties in vitro and increased long-term bone formation and early vascularization in *** effects of Mg coatings on osteoclasts have also been proven,which play a great role in osteoporotic *** addition,in order to obtain more biofunctions,other alloying elements such as copper have been added to the Mg ***,Mg-coated Ti acquired biofunctions including degradability,osteogenesis,angiogenesis and antibacterial properti

关键词： biofunction coating degradability magnesium osteogenesis review

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Methacrylated gelatin and platelet-rich plasma based hydrogels promote regeneration of critical-sized bone defects

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Regenerative Biomaterials 2024年第4期3卷 66-81页

作者： Shichao Lian Zhiyu Mu Zhengchao Yuan Muhammad Shafiq Xiumei Mo Weidong Mu department of Traumatic Orthopaedics Shandong Provincial HospitalShandong UniversityJinanShandong 250012China Zoucheng People’s Hospital ZouchengShandong 273500China department of Medical Physics and Biomedical Engineering University of LondonLondon WC1E 6BTUK State Key Laboratory for Modification of Chemical Fibers and Polymer Materials Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative MedicineCollege of Biological Science and Medical EngineeringDonghua UniversityShanghai 201620PR China Innovation Center of NanoMedicine(iCONM) Kawasaki Institute of Industrial PromotionKawasaki-KuKawasaki 210-0821Japan

Physiological repair of large-sized bone defects requires instructive scaffolds with appropriate mechanical properties,biocompatibility,biodegradability,vasculogenic ability and *** objective of this study was to fabricate in situ injectable hydrogels using platelet-rich plasma(PRP)-loaded gelatin methacrylate(GM)and employ them for the regeneration of large-sized bone *** performed various biological assays as well as assessed the mechanical properties of GM@PRP hydrogels alongside evaluating the release kinetics of growth factors(GFs)from *** GM@PRP hydrogels manifested sufficient mechanical properties to support the filling of the tissue *** biofunction assay,the GM@PRP hydrogels significantly improved cell migration and ***,transcriptome RNA sequencing of human umbilical vein endothelial cells and bone marrow-derived stem cells were performed to delineate vascularization and biomineralization abilities of GM@PRP *** GM@PRP hydrogels were subcutaneously implanted in rats for up to 4 weeks for preliminary biocompatibility followed by their transplantation into a tibial defect model for up to 8 weeks in *** defects treated with GM@PRP hydrogels manifested significant bone regeneration as well as angiogenesis,biomineralization,and collagen *** on the biocompatibility and biological function of GM@PRP hydrogels,a new strategy is provided for the regenerative repair of large-size bone defects.

关键词： large-sized bone defect repair platelet-rich plasma hydrogel tissue scaffold gelatin methacrylate

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Mechanobiomaterials: Harnessing mechanobiology principles for tissuerepair and regeneration

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Mechanobiology in Medicine 2024年第3期2卷 64-82页

作者： Xiao Lin Hua Yang Yi Xia Kang Wu Fengcheng Chu Huan Zhou Huajian Gao Lei Yang Orthopedic Institute Department of OrthopedicsThe First Affiliated HospitalSoochow UniversitySuzhouChina School of Mechanical Engineering Hebei University of TechnologyTianjinChina Center for Health Sciences and Engineering(CHSE) Hebei Key Laboratory of Biomaterials and Smart TheranosticsSchool of Health Sciences and Biomedical EngineeringHebei University of TechnologyTianjinChina Mechano-X Institute Applied Mechanics LaboratoryDepartment of Engineering MechanicsTsinghua UniversityBeijingChina

Mechanical stimuli are known to play critical roles in mediating tissue repair and regeneration. Recently, thisknowledge has led to a paradigm shift toward proactive programming of biological functionalities of biomaterialsby leveraging mechanics–geometry–biofunction relationships, which are beginning to shape the newly emergingfield of mechanobiomaterials. To profile this emerging field, this article aims to elucidate the fundamentalprinciples in modulating biological responses with material–tissue mechanical interactions, illustrate recentfindings on the relationships between material properties and biological responses, discuss the importance ofmathematical/physical models and numerical simulations in optimizing material properties and geometry, andoutline design strategies for mechanobiomaterials and their potential for tissue repair and regeneration. Giventhat the field of mechanobiomaterials is still in its infancy, this article also discusses open questions and challengesthat need to be addressed.

关键词： biomaterials geometry repair

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