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Clinical benefits of biochemical markers of bone turnover in Egyptian children with chronic liver diseases

World Journal of Gastroenterology 2007年第5期13卷 785-790页

作者： Karam A Mahdy Hanaa H Ahmed Fathia Mannaa Azza Abdel-Shaheed Medical biochemistry department National Research Centre Egypt Hormones department National Research Centre Egypt Medical Physiology department National Research Centre Egypt Child Health department National Research Centre Egypt

AIM： To investigate the association between serum insulin-like growth factor 1 （IGF-1）, osteocalcin, and parathyroid hormone （PTH） levels with the etiology and clinical condition of patients with chronic liver disease. METHODS： Eighty children with hepatocellular damage were divided into 3 groups according to the etiology of disease infection： bilharziasis （9 patients）, hepatitis B virus （HBV, 12 patients） and hepatitis C virus （HCV, 29 patients）. The Child score index was found as A in 24 patients, B in 22 patients, C in 4 patients. Thirty healthy children served as control ***, HBcAbIgM, HBcAbIgG, and anti-HCV were detected using ELISA technique. HCV-RNA was measured by reverse transcription polymerase chain reaction （RT-PCR）. Antibllharzial antibodies were detected by indirect haemagglutination test. Liver function tests were performed using autoanalyser. Serum IGF-1, osteocalcin and PTH levels were measured by ELISA technique. Abdominal ultrasonography was also conducted. RESULTS： Serum IGF-1 level was significantly lower in all patient groups with liver diseases, while serum osteocalcin and PTH levels were significantly elevated in patients with HBV and HCV infections compared with the control group. Serum osteocalcin and PTH concentrations were measured with the severity of liver disease from Child A to C. Child A patients unexpectedly showed significantly reduced IGF-1 levels in comparison to patients staged as Child B or C. Serum osteocalcin level was negatively correlated with albumin （14.7 ± 0.54 vs 3.6 ± 0.10, P 〈 0.05）, while that for PTH was positively correlated with total protein （70.1 ± 2.17 vs 6.7 ± 0.10, P 〈 0.05） in patients with HCV infection.

关键词： Liver disease Bone turnover Insulin-like growth factor-1 Osteocalcin Parathyroid hormone

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Vitamin D receptor expression in human bone tissue and dose-dependent activation in resorbing osteoclasts

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Bone research 2016年第3期4卷 164-173页

作者： Allahdad Zarei Alireza Morovat Kassim Javaid Cameron P Brown Botnar research centre Nuffield Department of Orthopaedics Rheumatology and Musculoskeletal Science University of Oxford Oxford UK Clinical biochemistry Oxford University Hospitals NHS Trust Oxford UK

The effects of vitamin D on osteoblast mineralization are well documented. Reports of the effects of vitamin D on osteoclasts, however, are conflicting, showing both inhibition and stimulation. Finding that resorbing osteoclasts in human bone express vitamin D receptor （VDR）, we examined their response to different concentrations of 25-hydroxy vitamin D3 [25（OH）D3] （100 or 500 nmol·L^-1） and 1,25-dihydroxy vitamin D3 [1,25（OH）2D3] （0.1 or 0.5 nmol·L^-1） metabolites in cell cultures. Specifically, CD14＋ monocytes were cultured in charcoal-stripped serum in the presence of receptor activator of nuclear factor kappa-B ligand （RANKL） and macrophage colony-stimulating factor （M-CSF）. Tartrate-resistant acid phosphatase （TRAP） histochemical staining assays and dentine resorption analysis were used to identify the size and number of osteoclast cells, number of nuclei per cell and resorption activity. The expression of VDR was detected in human bone tissue （ex vivo） by immunohistochemistry and in vitro cell cultures by western blotting. Quantitative reverse transcription-PCR （qRT-PCR） was used to determine the level of expression of vitamin D-related genes in response to vitamin D metabolites. VDR-related genes during osteoclastogenesis, shown by qRT-PCR, was stimulated in response to 500 nmol·L^-1 of 25（OH）D3 and 0.1-0.5 nmol·L^-1 of 1,25（OH）2D3, upregulating cytochrome P450 family 27 subfamily B member I （CYP27B1） and cytochrome P450 family 24 subfamily A member I （CYP24A1）. Osteoclast fusion transcripts transmembrane 7 subfamily member 4 （tm7sf4） and nuclear factor of activated T-cell cytoplasmic 1 （nfatcl） where downregulated in response to vitamin D metabolites. Osteoclast number and resorption activity were also increased. Both 25（OH）D3 and 1,25（OH）2D3 reduced osteoclast size and number when co-treated with RANKL and M-CSF. The evidence for VDR expression in resorbing osteoclasts in vivo and low-dose effects of 1,25（OH）2D3 on osteoclasts in vitro may therefore provide

关键词： Vitamin D receptor expression in human bone tissue and dose-dependent activation in resorbing osteoclasts bone

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Prenatal exposure to the fluoride containing psychiatric drug fluoxetine and anti-oxidative alterations in the neonatal rat brain

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BIOCELL 2019年第2期43卷 65-71页

作者： RAMESA SHAFI BHAT AMINA EL GEZEERY ABIR BEN BACHAN MONA AWAD ALONAZI LEENA SALEH ALSUHAIBANI AFAF EL-ANSARY biochemistry department College of ScienceKing Saud University Human Genetic department Medical Research instituteAlexandria UniversityEgypt Central Laboratory Female Centre for Scientific and Medical StudiesKing Saud UniversitySaudi Arabia Medicinal Chemistry department National Research CentreDokkiCairoEgypt

Fluoride is a key ingredient of many psychiatric drugs like fluoxetine(Prozac■Fluoxetine■).Pregnant women frequently use this drug as they suffer from depression and anxiety disorders during this *** is able to reach the fetus through the placenta and passes to the newborn through *** the present study,female Wistar rats were treated with 5,10,and 20 mg/L fluoxetine(containing 94% fluorides)from pregnancy day 10 to day *** delivery,the levels of the enzymatic antioxidants in the brain of their offspring at postnatal day 2 were *** results showed that,in all fluoxetine exposed groups compared with the control group,there was a significant decrease(P0.05)in the activity of malondialdehyde and creatine *** results suggest that fluoxetine may be a developmental neurotoxicant due to presence of fluoride hence must be used carefully during pregnancy.

关键词： Antidepressant Brain Neonate Prozac Oxidative stress

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In vitro assessment of the synergism between extracts of Cocos nucifera husk and some standard antibiotics

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Asian Pacific Journal of Tropical Biomedicine 2017年第4期7卷 306-313页

作者： Taiwo Adesola Akinyele Etinosa Ogbomoede Igbinosa David Ayinde Akinpelu Anthony Ifeanyin Okoh SAMRC Microbial Water Quality Monitoring centre University of Fort Hare Applied and Environmental Microbiology research Group Department of Biochemistry and MicrobiologyUniversity of Fort Hare

Objective: To evaluate the interactions between the crude extracts of Cocos nucifera(***) and six front line antibiotics(ampicillin sodium salt, penicillin G sodium,amoxicillin, chloramphenicol, ciprofloxacin and tetracycline hydrochloride), against some bacterial pathogens linked with human ***: The pulverized husk of *** was dissolved in 95% n-hexane and extracted using Soxhlet extraction method and sterile distilled water(aqueous).The antibacterial susceptibility of the crude extracts of *** was tested against environmental and clinical strains(6) obtained from the South African Bureau of Standards(SABS), Vibrio(6) and Listeria pathogens(6).The agar-well diffusion method was used for screening the extracts for their antibacterial *** minimum inhibitory concentration and minimum bactericidal concentration of the extracts were ***-kill assay was used to evaluate bactericidal and/or bacteriostatic *** synergistic effect of the crude extracts and antibiotics was assessed and evaluated by adopting the checkerboard ***: With the time-kill assay, the highest bactericidal activity was observed on Vibrio fluvialis EL041 with a-5.6 ± 0.2 log_(10)CFU/mL decrease in cell density as a result of the combination of the extracts and chloramphenicol at two-fold minimum inhibitory *** using the time-kill assay constituted about 72%, while indifference constituted about 28%.The checkerboard method revealed synergistic interaction in 67% of the combinations, and indifference in 33%.There was no specificity in the observed synergy to a particular class of ***: This investigation suggests the crude extracts of *** to be a potential broad spectrum antimicrobial ***, further study is needed to isolate the pure compounds from these crude extracts.

关键词： Antibacterial Inhibition zone Synergism Indifference Antibiotics Time-kill assay

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LSM12 facilitates the progression of colorectal cancer by activating the WNT/CTNNB1 signaling pathway

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Oncology research 2022年第6期30卷 289-300页

作者： YAN ZHUANG CHUNLAN NING PENGFEI LIU YANPENG ZHAO YUE LI ZHENCHI MA LULING SHAN YINGZHE PIAO PENG ZHAO XUN JIN department of Colorectal Oncology Tianjin Medical University Cancer Institute&HospitalNational Clinical Research Center for CancerTianjin’s Clinical Research Center for CancerKey Laboratory of Cancer Prevention and TherapyTianjin300060China department of biochemistry and Molecular Biology Tianjin Medical University Cancer Institute and HospitalNational Clinical Research Center for CancerTianjin’s Clinical Research Center for CancerKey Laboratory of Cancer Prevention and TherapyTianjin300060China Tianjin Medical University Tianjin300070China department of Oncology Tianjin Academy of Traditional Chinese Medicine Affiliated HospitalTianjin300120China Tianjin Marvel Medical Laboratory Tianjin Marvelbio Technology Co.Ltd.Tianjin300381China department of Gastro Colorectal Oncology Tianjin Cancer Hospital Airport HospitalTianjin300304China department of Neuro-Oncology and Neurosurgery Tianjin Medical University Cancer Institute&HospitalNational Clinical Research Center for CancerKey Laboratory of Cancer Prevention and Therapy TianjinTianjin’s Clinical Research Center for CancerTianjin300060China

Aberrant activation of the WNT signaling pathway is a joint event in colorectal cancer(CRC),but the molecular mechanism is still ***,RNA-splicing factor LSM12(like-Sm protein 12)is highly expressed in CRC *** study aimed to verify whether LSM12 is involved in regulating CRC progression via regulating the WNT signaling ***,we found that LSM12 is highly expressed in CRC patient-derived tissues and ***12 is involved in the proliferation,invasion,and apoptosis of CRC cells,similar to the function of WNT signaling in ***,protein interaction simulation and biochemical experiments proved that LSM12 directly binds to CTNNB1(also known asβ-Catenin)and regulates its protein stability to affect the CTTNB1-LEF1-TCF1 transcriptional complex formation and the associated WNT downstream signaling ***12 depletion in CRC cells decreased the in vivo tumor growth through repression of cancer cell growth and acceleration of cancer cell *** together,we suggest that the high expression of LSM12 is a novel factor leading to aberrant WNT signaling activation,and that strategies targeting this molecular mechanism may contribute to developing a new therapeutic method for CRC.

关键词： CRC LSM12 WNT CTNNB1 Apotosis

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MYCOPLASMA HYORHINIS IN GASTRIC cancer

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Chinese Journal of cancer research 2002年第2期14卷 84-87页

作者：季加孚张霁寿成超王怡徐光炜 department of Surgery Beijing Cancer HospitalBeijing 100036 department of biochemistry Peking University School of OncologyBeijing Cancer HospitalBeijing 100036

Objective: To determine the prevalence of Mycoplasma hyorhinis in archived paraffin-embedded gastric cancer tissue. Methods: The antigen recognized by anti-tumor monoclonal antibody PD4 was identified as P40 (a specific Mycoplasma hyorhinis protein). We constructed a tissue-microarray of high density containing 105 gastric cancer samples, 101 non-tumor margin samples and 62 benign gastric disease samples for detecting Mycoplasma hyorhinis using Immunohistochemistry. Results: The infection rate of M. hyorhinis was 54.1% (53/98) in gastric cancer samples, 51.7%(45/87) in non-tumor margin samples and 15.8% (9/57) in benign disease samples. The difference in infection rate between gastric cancer and benign gastric disease has statistical significance (P=0.001). Highly differentiated adenocarcinomas have a greater chance (84.6%) to be infected with M. hyorhinis than poorly differentiated ones (45.5%) (Pcancer than in other tastric diseases, which suggests the association between Mycoplasma infection and gastric cancer. Whether M. hyorhinis has oncogenic potential needs to be elucidated.

关键词： Mycoplasma Gastric cancer Tissue microassay

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Phytochemically Functionalized Cu and Ag Nanoparticles Embedded in MWCNTs for Enhanced Antimicrobial and Anticancer Properties

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Nano-Micro Letters 2016年第2期8卷 120-130页

作者： S.Yallappa J.Manjanna B.L.Dhananjaya U.Vishwanatha B.Ravishankar H.Gururaj P.Niranjana B.S.Hungund department of Industrial Chemistry Kuvempu University department of Chemistry Rani Channamma University Toxicology and Drug Discovery centre for Emerging Technologies Jain University SDM centre for research in Ayurveda and Allied Sciences department of Electronics Kuvempu University department of biochemistry Kuvempu University department of Biotechnology B.V.B.College of Engineering & Technology

Nanomedicine is an emerging field concerned with the use of precision engineered nanomaterials, which leads to the development of novel remedial and diagnostic modalities for human use. In this study, Cu(NO_3)_2 and Ag NO_3 precursors were reduced to copper nanoparticles(Cu NPs) and silver nanoparticles(Ag NPs) using Terminalia arjuna bark extracts under microwave irradiation in the presence of well-dispersed multi-walled carbon nanotubes(MWCNTs) in aqueous medium. The formation of Cu NPs or Ag NPs and their functionalization with MWCNTs via bioactive molecules of plant extract were evidenced from UV–Vis spectra, XRD, FTIR, FESEM, EDX, and TEM images. The phytochemically functionalized Cu-MWCNTs and Ag-MWCNTs nanomaterials showed enhanced biocide activity, and the inhibitory activity for bacteria was higher than that of fungus. Furthermore, these biohybrid nanomaterials are non-toxic to normal epithelial cells(Vero), whereas they are highly toxic for tested human cancer cells of MDA-MB-231, He La, Si Ha, and Hep-G2. The cell viability was found to decrease with the increasing dose from 10 to 50 lg m L^(-1), as well as incubation time from 24 to 72 h. For instance, the cell viability was found to be *91 % for normal Vero cells and *76 % for cancer cells for lower dose of 10 lg m L^(-1).

关键词： Cu-MWCNTs Ag-MWCNTs Terminalia arjuna bark extract Antimicrobial activity Anticancer activity

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Import Determinants of Organelle-Specific and Dual Targeting Peptides of Mitochondria and Chloroplasts in Arabidopsis thaliana

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Molecular Plant 2014年第1期7卷 121-136页

作者： Changrong Ge Erika Spanning Elzbieta Glaser Ake Wieslander department of biochemistry and Biophysics Center for Biomembrane Research Stockholm University SE-106 91 Stockholm Sweden Medical Inflammation research Department of Medical Biochemistry and Biophysics Karolinska Institute SE-171 77 Stockholm Sweden

Most of the mitochondrial and chloroplastic proteins are synthesized in the cytosol as precursor proteins carrying an N-terminal targeting peptide （TP） directing them specifically to a correct organelle. However, there is a group of proteins that are dually targeted to mitochondria and chloroplasts using an ambiguous N-terminal dual targeting peptide （dTP）. Here, we have investigated pattern properties of import determinants of organelle-specific TPs and dTPs combining mathematical multivariate data analysis （MVDA） with in vitro organellar import studies. We have used large datasets of mitochondrial and chloroplastic proteins found in organellar proteomes as well as manually selected data sets of experimentally confirmed organelle-specific TPs and dTPs from Arabidopsis thaliana. Two classes of organelle-specific TPs could be distinguished by MVDA and potential patterns or periodicity in the amino acid sequence contributing to the separation were revealed, dTPs were found to have intermediate sequence features between the organelle-specific TPs. Interestingly, introducing positively charged residues to the dTPs showed clustering towards the mitochondrial TPs in silico and resulted in inhibition of chloroplast, but not mitochondrial import in in vitro organellar import studies. These findings suggest that positive charges in the N-terminal region of TPs may function as an ＇avoidance signal＇ for the chloroplast import.

关键词： dual targeting ambiguous targeting signal mitochondria chloroplast protein import partial least square discriminant analysis Arabidopsis thaliana.

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Transperineal prostate biopsies for diagnosis of prostate cancer are well tolerated： a prospective study using patient-reported outcome measures

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Asian Journal of Andrology 2017年第1期19卷 62-66页

作者： Karan Wadhwa Lina Carmona-Echeveria Timur Kuru Gabriele Gaziev Eva Serrao Deepak Parasha Julia Frey Ivailo Dimov Jonas Seidenader Pete Acher Gordon Muir Andrew Doble Vincent Gnanapragasam Boris Hadaschik Christof Kastner department of Urology Addenbrookes Hospital Cambridge UK department of Urology University Hospital Heidelberg Germany department of biochemistry University of Cambridge Cambridge UK cancer research centre and Statistics and Epidemiology Unit Division of Health Sciences Warwick Medical School University of Warwick Coventry UK department of Urology Southend University Hospital Southend UK department of Urology Kings College Hospital London UK CamPARI Prostate cancer Clinic Addenbrookes Hospital Cambridge UK.

We aimed to determine short-term patient-reported outcomes in men having general anesthetic transperineal （TP） prostate biopsies. A prospective cohort study was performed in men having a diagnostic TP biopsy. This was done using a validated and adapted questionnaire immediately post-biopsy and at follow-up of between 7 and 14 days across three tertiary referral hospitals with a response rate of 51.6%. Immediately after biopsy 43/201 （21.4%） of men felt light-headed, syncopal, or suffered syncope. Fifty-three percent of men felt discomfort after biopsy （with 95% scoring 〈5 in a 0-10 scale）. Twelve out of 196 men （6.1%） felt pain immediately after the procedure. Despite a high incidence of symptoms （e.g., up to 75% had some hematuria, 47% suffered some pain）, it was not a moderate or serious problem for most, apart from hemoejaculate which 31 men suffered. Eleven men needed catheterization （5.5%）. There were no inpatient admissions due to complications （hematuria, sepsis）. On repeat questioning at a later time point, only 25/199 （12.6%） of men said repeat biopsy would be a significant problem despite a significant and marked reduction in erectile function after the procedure. From this study, we conclude that TP biopsy is well tolerated with similar side effect profiles and attitudes of men to repeat biopsy to men having TRUS biopsies. These data allow informed counseling of men prior to TP biopsy and a benchmark for tolerability with local anesthetic TP biopsies being developed for clinical use.

关键词： attitude to rebiopsy patient-reported outcome measures patient satisfaction prostate cancer diagnosis transperineal biopsies transrectal biopsies

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The substrates of Plk1, beyond the functions in mitosis

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Protein & Cell 2010年第11期1卷 999-1010页

作者： X.Shawn Liu Bing Song Xiaoqi Liu department of biochemistry Purdue UniversityWest LafayetteIndiana 47907USA department of Biological Sciences Purdue UniversityWest LafayetteIndiana 47907USA Center for cancer research Purdue UniversityWest LafayetteIndiana 47907USA

Polo-like kinase 1(Plk1)is a key regulator of cell division in eukaryotic *** this short review,we briefly summarized the well-established functions modulated by Plk1 during *** mitosis,we focused mainly on the unexpected processes in which Plk1 emerges as a critical player,including microtubule dynamics,DNA replication,chromosome dynamics,p53 regulation,and recovery from the G2 DNA-damage *** discussion is mainly based on the critical substrates targeted by Plk1 during these cellular events and the functional significance associated with each phosphorylation event.

关键词： Polo-like kinase 1 phosphorylation substrates

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