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Can antiretroviral therapy be tailored to each human immunodeficiency virus-infected individual? Role of pharmacogenomics

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World Journal of virology 2015年第3期4卷 169-177页

作者： Victor Asensi Julio Collazos Eulalia Valle-Garay Infectious Diseases-HIV Unit Hospital Universitario Central de AsturiasOviedo University School of Medicine Infectious Diseases Unit Hospital de Galdácano Biochemistry and Molecular Biology department Oviedo University School of Medicine

Pharmacogenetics refers to the effect of single nucleotide polymorphisms(SNPs) within human genes on drug therapy outcome. Its study might help clinicians to increase the efficacy of antiretroviral drugs by improving their pharmacokinetics and pharmacodynamics and by decreasing their side effects. HLAB*5701 genotyping to avoid the abacavir-associated hypersensitivity reaction(HSR) is a cost-effective diagnostic tool, with a 100% of negative predictive value, and, therefore, it has been included in the guidelines for treatment of human immunodeficiency virus(HIV) infection. HALDRB*0101 associates with nevirapine-induced HSR. CYP2B6 SNPs modify efavirenz plasma levels and their genotyping help decreasing its central nervous system, hepatic and HSR toxicities. Cytokines SNPs might influence the development of drug-associated lipodystrophy. APOA5, APOB, APOC3 and APOE SNPs modify lipids plasma levels and might influence the coronary artery disease risk of HIV-infected individuals receiving antiretroviral therapy. UGT1A1*28 and ABCB1(MDR1) 3435 C > T SNPs modify atazanavir plasma levels and enhance hyperbilirubinemia. Much more effort needs to be still devoted to complete large prospective studies with multiple SNPs genotyping in order to reveal more clues about the role played by host genetics in antiretroviral drug efficacy and toxicity.

关键词： Pharmacogenomics Pharmacokinetics Antiretroviral drugs Adverse effects Human immunodeficiency virus infection Single nucleotide polymorphisms

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狂犬病暴露前免疫:一项系统综述

中华实验和临床病毒学杂志 2019年第5期33卷 535-535页

作者： Jocelyn A Kessels Sergio Recuenco Ana Maria Navarro-Vela Raffy Deray Marco Vigilato Hildegund E rtl David Durrheim Helen Rees Louis H Nel Bernadette Abela-Ridder Deborah Briggsk 吕新军(编译) School of Veterinary Science University of Queensland Gatton Campus Via Warrego Highway Gatton Queensland 4343 Australia . National Centre for Public Health Instituto Nacional de Salud Lima Peru . Directorate General of Strategic Interventions in Public Health Ministry of Health Lima Peru . Diseases Prevention and Control Bureau Department of Health Manilla Philippines . Veterinary Public Health Unit Pan American Health Organisation-World Health Organisation Rio de Janeiro Brazil . Wistar Institute Vaccine Center Philadelphia United States of America (USA). Hunter Medical Research Institution University of Newcastle Newcastle Australia . Wits Reproductive Health and HIV Institute University of Witwatersrand Johannesburg South Africa . department of Microbiology and Plant Pathology University of Pretoria Pretoria South Africa . Neglected Zoonotic Diseases World Health Organization Geneva Switzerland . College of Veterinary Medicine Kansas State University Manhattan USA .

为了综述狂犬病暴露前免疫的安全性和免疫原性(包括加速程序、与其他疫苗和增强剂的联合应用)、其成本效益和使用建议。在PubMed、Centre for Agriculture and Biosciences International、Cochrane Library、Web of Science databases... 详细信息

为了综述狂犬病暴露前免疫的安全性和免疫原性(包括加速程序、与其他疫苗和增强剂的联合应用)、其成本效益和使用建议。在PubMed、Centre for Agriculture and Biosciences International、Cochrane Library、Web of Science databases检索2007年至2016年1月29日期间发表的关于狂犬病暴露前免疫的论文,回顾在秘鲁和菲律宾开展的暴露前免疫运动的现场数据。

关键词：暴露前免疫系统综述狂犬病成本效益 Cochrane 免疫原性增强剂

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Substrate specificity of avian influenza H5N1 neuraminidase

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World Journal of virology 2014年第4期3卷 30-36页

作者： Naruthai Onsirisakul Shin-ichi Nakakita Chompunuch Boonarkart Alita Kongchanagul Ornpreya Suptawiwat Pilaipan Puthavathana Krisada Chaichuen Kanokwan Kittiniyom Yasuo Suzuki Prasert Auewarakul department of Clinical Microbiology and Applied Technology Faculty of Medical TechnologyMahidol UniversityBangkok 73170Thailand department of Microbiology Faculty of Medicine Siriraj HospitalMahidol UniveristyBangkok 10700Thailand Division of Functional Glycomics Life Sciences Research CenterKagawa UniversityTakamatsu 761-0793Japan Institute of Molecular Biosciences Mahidol UniversityNakhon Pathom 73170Thailand Faculty of Veterinary Science Mahidol UniversityNakorn Pathom 73170Thailand College of Life and Health Sciences Chubu UniversityKasugai 487-8501Japan

AIM: To characterise neuraminidase(NA) substrate specificity of avian influenza H5N1 strains from humans and birds comparing to seasonal influenza ***: Avian influenza H5N1 strains from humans and birds were recruited for characterising their NA substrate specificity by using a modified commercial fluorescence Amplex Red assay. This method can identify the preference of α2,6-linked sialic acid or α2,3-linked sialic acid. Moreover, to avoid the bias of input virus, reverse genetic virus using NA gene from human isolated H5N1 were generated and used to compare with the seasonal influenza virus. Lastly, the substrate specificity profile was further confirmed by high-performance liquid chromatography(HPLC) analysis of the enzymatic product. RESULTS: The H5N1 NA showed higher activity on α2,3-linked sialic acid than α2,6-linked(P < 0.0001). To compare the NA activity between the H5N1 and seasonal influenza viruses, reverse genetic viruses carrying the NA of H5N1 viruses and NA from a seasonal H3N2 virus was generated. In these reverse genetic viruses, the NA activity of the H5N1 showed markedly higher activity against α2,3-linked sialic acid than that of the H3N2 virus, whereas the activities on α2,6-linkage were comparable. Interestingly, NA from an H5N1 human isolate that was previously shown to have heamagglutinin(HA) with dual specificity showed reduced activity on α2,3-linkage. To confirm the substrate specificity profile, HPLC analytic of enzymatic product was performed. Similar to Amplex red assay, H5N1 virus showed abundant preference on α2,3-linked sialic ***: H5N1 virus maintains the avian specific NA and NA changes may be needed to accompany changes in HA receptor preference for the viral adaptation to humans.

关键词： H5N1 avian influenza virus Neuraminidase Sialic acid Adaptation Substrate preference

噬菌体作为模式生物在蛋白表达和病毒学方面的应用

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中国微生态学杂志 2012年第5期24卷 466-469页

作者：柳鹏程乔涵刘斐斐刘毕胜吴倩妮张燕燕单金玉魏莲花马兴铭兰州大学基础医学院免疫学研究所甘肃兰州730000 department of Infection Immunity and Inflam-mationUniversity of LeicesterLeicester UK LE1 9HN 甘肃省人民医院检验科甘肃兰州730000

近年来,随着噬菌体研究的不断深入,人们逐渐意识到噬菌体巨大的应用潜力。噬菌体表面展示技术、噬菌体抗体库、噬菌体核酸疫苗是将噬菌体作为载体而实现基因型与表型的结合,在病毒学方面噬菌体常被作为模式生物应用于多个研究领域。本... 详细信息

近年来,随着噬菌体研究的不断深入,人们逐渐意识到噬菌体巨大的应用潜力。噬菌体表面展示技术、噬菌体抗体库、噬菌体核酸疫苗是将噬菌体作为载体而实现基因型与表型的结合,在病毒学方面噬菌体常被作为模式生物应用于多个研究领域。本文就以上各点作一综述。

关键词：噬菌体展示技术抗体库核酸疫苗模式生物

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Human T-lymphotropic virus proteins and post-translational modification pathways

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World Journal of virology 2012年第4期1卷 115-130页

作者： Carlo Bidoia Centre for Research in Infectious Diseases School of Medicine and Medical ScienceUniversity College DublinBelfieldDublin 4Ireland department of Surgical and Morphologic Sciences University of InsubriaPadiglione Biffi via Ottorino Rossi921100 VareseItaly

Cell life from the cell cycle to the signaling transduction and response to stimuli is finely tuned by protein post-translational modifications(PTMs).PTMs alter the conformation,the stability,the localization,and hence the pattern of interactions of the targeted *** pathways involve the activation of enzymes,like kinases,ligases and transferases,that,once activated,act on many proteins simultaneously,altering the state of the cell and triggering the processes they are involved *** enter a balanced system and hijack the cell,exploiting the potential of PTMs either to activate viral encoded proteins or to alter cellular pathways,with the ultimate consequence to perpetuate through their *** T-lymphotropic virus type 1(HTLV-1)is known to be highly oncogenic and associates with adult T-cell leukemia/lymphoma,HTLV-1-associated myelopathy/tropical spastic paraparesis and other inflammatory pathological ***-1 protein activity is controlled by PTMs and,in turn,viral activity is associated with the modulation of cellular pathways based on *** knowledge is acquired about the PTMs involved in the activation of its proteins,like Tax,Rex,p12,p13,p30,HTLV-I basic leucine zipper factorand ***,more has to be understood at the biochemical level in order to counteract the associated fatal *** review will focus on known PTMs that directly modify HTLV-1 components and on enzymes whose activity is modulated by viral proteins.

关键词： Human T-Lymphotropic virus Tax Rev p12 p13 Gag Post-translational modification

利用人摄金蛋白(METALLOTHIONEIN)启动子和牛乳突瘤病毒在哺乳动物细胞中表达乙型肝炎表面抗原

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病毒学报 1986年第1期 1-9+97页

作者：谢彦博,Peter M. Price,John Vnek,Alfred M.Prince 卫生部北京生物制品研究所 Department of Biochemistry Mt. Sinai Medical Center New York USA Departmnt of Viroiogy The Lindsley F. Kimball Research Institute of New York Blood Center New York USA Departmnt of Viroiogy The Lindsley F. Kimball Research Institute of New York Blood Center New York USA

用人Metallothioenin-Ⅱ启动子、乙型肝炎表面抗原基因,SV40早期基因的编接位点和多聚A位点构成了表达组件,然后插入到经改造过的BpV-1质粒中。所得质粒pdMTsAg-5转染小鼠C127细胞得到转化克隆。Ausria Ⅱ检测证明13株中有12株能产生HB... 详细信息

用人Metallothioenin-Ⅱ启动子、乙型肝炎表面抗原基因,SV40早期基因的编接位点和多聚A位点构成了表达组件,然后插入到经改造过的BpV-1质粒中。所得质粒pdMTsAg-5转染小鼠C127细胞得到转化克隆。Ausria Ⅱ检测证明13株中有12株能产生HBsAg。对其中一株进行HBsAg收率观察,隔天收获为292.6～525.8μg/升,每天收获为200.9～369.0μg/升,可连续收获60天以上。经重金属离子诱导后,收率增至583～854.4μg/升。培养液经超滤浓缩和两次密梯离心后,可集中为一个狭窄的峰,顶峰的Ausria Ⅱ cpm为1.05×107,密度为1.20g/ml。

关键词：哺乳动物细胞基因工程人Metallothionein启动子牛乳突瘤病毒

Semen lactoferrin promotes CCL20 production by epithelial cells: Involvement in HIV transmission

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World Journal of virology 2014年第2期3卷 11-17页

作者： Alan Grupioni Loureno Marilena Chinalli Komesu Alcyone Artioli Machado Silvana Maria Quintana Thomas Bourlet Bruno Pozzetto Olivier Delézay Division of Infectious Diseases Department of Internal MedicineSchool of Medicine of Ribeir?o PretoUniversity of S?o PauloRibeir?o Preto14049-900 S?o PauloBrazil department of Morphology Stomatology and PhysiologySchool of Dentistry of Ribeir?o PretoUniversity of S?o PauloRibeir?o Preto14049-900 S?o PauloBrazil EA3064 Groupe Immunité des Muqueuses et Agents Pathogènes(GIMAP)Faculty of Medicine Jacques LisfrancUniversity of Lyon42023 Saint-EtienneFrance

AIM: To study the effect of seminal plasma on Chemokine(C-C motif) ligand 20(CCL20) production by epithelial cells and its relationship with ***: HEC-1A cells, a cell line derived from a monostratified endocervical epithelium, were incubated with samples of seminal plasma(diluted 1:10 in culture medium) recovered from human immunodeficiency virus(HIV) seronegative(HIV-) or HIV seropositive(HIV+) subjects. Recombinant human interleukin 1 beta(IL-1β) was used as positive control, and culture medium only as negative control. The measurement of CCL20 production in the supernatants of HEC-1A cells and of lactoferrin in seminal plasma was determined by enzyme-linked immunosorbent assay techniques. A fractionation of seminal plasma proteins was performed by ion exchange chromatography on a pool of seminal plasma specimens from HIV- subjects. Each fraction was tested for its ability to stimulate the production of CCL20 by HEC-1A cells and for its lactoferrin concentration. The HIV viral load in seminal plasma samples from HIV+ patients was measured using the HIV-Monitor kit(Roche Diagnostic Systems, Branchburg, NJ, United States).RESULTS: The positive control IL-1β was responsible for an increase of 11.36 ± 3.36 times in the production of CCL20. Stimulation of HEC-1A cells was performed in 34 seminal plasma samples(22 from HIV+ subjects and 12 from HIV- subjects). The mean production of CCL20 by HEC-1A in presence of seminal plasma from HIV- and HIV+ subjects was respectively 5.38 ± 0.91 and 7.57 ± 3.26 times higher than that obtained with the untreated cells(P < 0.05 between the two groups). Using the same 34 specimens of seminal plasma, no correlation was observed between the concentration of total proteins in seminal plasma and their ability to stimulate the secretion of CCL20 by HEC-1 cells. In contrast, the ability to produce CCL20 by HEC-1A cells correlated to the concentration of lactoferrin in the seminal plasma samples(r coefficient = 0.56; CI: 0.26

关键词： Human immunodeficiency virus/acquired immunodeficiency syndrome Sexual transmission Seminal plasma CCL20 Lactoferrin Endocervical epithelial cells

Searching for nuclear export elements in hepatitis D virus RNA

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World Journal of virology 2013年第3期2卷 123-135页

作者： Natália Freitas Celso Cunha department of Microbiology Molecular Genetics and Immunology Kansas University Medical Center Rainbow Boulevard Kansa KS 66160 United States Medical Microbiology Unit Center for Malaria and Tropical Diseases Institute of Hygiene and Tropical Medicine Nova University of Lisbon 1349-008 Lisbon Portugal

AIM: To search for the presence of cis elements in hepatitis D virus(HDV) genomic and antigenomic RNA capable of promoting nuclear export. METHODS: We made use of a well characterized chloramphenicol acetyl-transferase reporter system based on plasmid p DM138. Twenty c DNA fragments corresponding to different HDV genomic and antigenomic RNA sequences were inserted in plasmid pD M138, and used in transfection experiments in Huh7 cells. The relative amounts of HDV RNA in nuclear and cytoplasmic fractions were then determined by realtime polymerase chain reaction and Northern blotting. The secondary structure of the RNA sequences that displayed nuclear export ability was further predicted using a web interface. Finally, the sensitivity to leptomycin B was assessed in order to investigate possible cellular pathways involved in HDV RNA nuclear ***: Analysis of genomic RNA sequences did not allow identifying an unequivocal nuclear export element. However, two regions were found to promote the export of reporter m RNAs with efficiency higher than the negative controls albeit lower than the positive control. These regions correspond to nucleotides 266-489 and 584-920, respectively. In addition, when analyzing antigenomic RNA sequences a nuclear export element was found in positions 214-417. Export mediated by the nuclear export element of HDV antigenomic RNA is sensitive to leptomycin B suggesting a possible role of CRM1 in this transport pathway. CONCLUSION: A cis-acting nuclear export element is present in nucleotides 214-417 of HDV antigenomic RNA.

关键词： Hepatitis D virus Genomic RNA Antigenomic RNA Nuclear export Nuclear export element

In memory of Dr. Azar Andami, an eminent researcher and scholar at the Pasteur Institute of Iran

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History & Philosophy of Medicine 2023年第4期5卷 15-18页

作者： Zahra Tahmasebi Ashtiani Ehsan Mostafavi department of Epidemiology and Biostatics Research Centre for Emerging and Reemerging Infectious DiseasesPasteur Institute of IranTehran ***Iran National Reference Laboratory for Plague Tularemia and Q FeverResearch Centre for Emerging and Reemerging Infectious DiseasesPasteur Institute of IranAkanluKabudar-AhangHamadan ***Iran

This paper focuses on the remarkable contributions and achievements of *** Andami(1926–1984),a renowned physician and specialist at the Pasteur Institute of *** conducted extensive research on infectious diseases,particularly cholera,utilizing her knowledge in medicine,bacteriology,and laboratory *** played a crucial role in the mass production of the cholera vaccine at the Pasteur Institute of Iran and in controlling the spread of communicable diseases both locally and *** paper aims to highlight her remarkable works and accomplishments.

关键词： Pasteur history Pasteur institute of Iran cholera Iran