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THE STUDY OF HUMAN TRANSFER FACTOR

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Science in China,Ser.B 1985年第4期28卷 394-401页

作者：李在连 microbiology department Changwei Medical College Weifang

In this paper, the study of human transfer factor is reported. We established a negative pressure dialysis method instead of ordinary dialysis for treatment of the crude leukocyte extract. Dialysate is rapidly obtained in only 5h so that a large volume of preparation is easier to handle and the chance of contamination avoided. When humau TF was incubated With human cord blood T-lymphocytes and pig lymphocytes, a very high biological activity on SRBC rosette enhancement and an increase in electrophoresis rate appeared. It suggests that these assays may be used as in vitro method of evaluation of TF activity. In our clinic, TF has been in clinical trials for 5 years and has now been administered to a large number of patients with a variety of diseases, in which cell-mediated immune responses have been compromised. We observe that TF has served an efficient immunopotentiating or immunemodulation agent.

关键词： THE STUDY OF HUMAN TRANSFER FACTOR TF RFC

Preparation and Characterization of Antibacterial Zn^(2+)-Exchanged Montmorillonites

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Journal of Wuhan University of Technology(Materials Science) 2010年第5期25卷 725-729页

作者：施庆珊谭绍早 Guangdong Institute of microbiology department of Chemistry Jinan University

Zn-montmorillonites（Zn-MMTs） as antibacterial compounds were prepared by an ion-exchange reaction. The reaction time, initial pH value and molar ratios of CEC influencing zinc content in Zn-MMTs were investigated, and Zn-MMTs were characterized by means of EDX, XRD, XPS, and SEM. The results of bacterial growth tests were confirmed by determination of the minimum inhibition concentrations （MICs） and minimum bactericidal concentrations （MBCs）. The experimental results show that the zinc is confirmed as bivalent zinc state, the d001 basal spacing of Zn-MMTs is enlarged with the enhancement of the zinc content, and the particles of Zn-MMTs are formed with irregular shape. Moreover, the antibacterial activity of Zn-MMTs increases with increasing the zinc content, and Zn-MMT-3 containing 6.76 mass% of zinc exhibits optimum antibacterial activity against Escherichia coli and Staphylococcus aureus.

关键词： Zn^2+-exchanged montmorillonites preparation chemical composition microstructure antibacterial activity

Identification of Arbuscular mycorrhizal multiplicity in the saline-sodic soils

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International Journal of Agricultural and Biological Engineering 2014年第2期7卷 56-67页

作者： Srimathi Priya L Kumutha K Arthee R Pandiyarajan P department of Agricultural microbiology Tamil Nadu Agricultural UniversityCoimbatore-641003India department of Agricultural microbiology Anbil Dharmalingam Agricultural CollegeTrichy-641009India

This study focused on the Arbuscular mycorrhizal(AM)fungal diversity in the saline-sodic soils based on native spore density and most probable number(MPN)*** through spore morphology showed existence of five genera in the various crop *** physico-chemical analysis of the native soils revealed that they were saline-sodic with pH ranging from(8.7±0.5)to(9.5±0.6)and habituated five different genera of AM fungi including Glomus,Scutellospora,Acaulospora,Sclerocystis and *** location revealed presence of varied species of AM fungus namely Acaulospora and Glomus in rhizosphere of maize;Scutellospora and Glomus in tulsi;four isolates of Glomus in onion;Glomus and Sclerocystis in guava;three isolates of Glomus in rice;Glomus in neem and Gigaspora and Glomus in *** molecular identification through nested PCR analysis showed amplification of 600 bp size in SSU rDNA gene in samples A and C(predominated by Acaulospora and Glomus mosseae respectively).

关键词： AM fungal identification saline-sodic soil crop rhizosphere spore density nested PCR technology glomalean fungus

Design of a multiplex PCR method for detection of toxigenicpathogenic in Vibrio cholerae

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Asian Pacific Journal of Tropical Medicine 2013年第2期6卷 115-118页

作者： Abbas Ali Imani Fooladi Iman Islamieh D Hosseini Doust R Karami A Marashi SM applied microbiology Research Center Baqiyatallah University of Medical SciencesTehranIran department of microbiology Faculty of Advance Sciences and TechnologyIslamic Azad University-Pharmaceutical Sciences BranchTehranIran Molecular Biology Research Center Baqiyatallah University of Medical SciencesTehranIran department of microbiology and Immunology Babol University of Medical SciencesBabolIran

Objective:To study virulence and regulatory genes(hlyA,ctxB,tcpI) in clinical strains of Vibrio ckolerae(***),***:Three important genes,tepI,hlyA and ctxB were used for detection of toxigenic and pathogenic *** by chain reaction assay method. Results:According to the results of the PCR,the incidence of hlyA,tcpI,and ctxB genes in clinical isolates was obtained as 94.7%(72 sample),90.8%(69 sample),and 92.1%(70 sample), *** strains possessed all genes except ctxB,six strains possessed all genes except tcpI,four strains possessed all genes except hlyA,one strain possessed only hlyA and 60 strains contained a combination of three *** hlyA,ctxB and tcpI,Conclusions:Result show that this method could be reliable to detect toxigenic-pathogenic strains of *** in Iran.

关键词： Vibrio cholerae Multiplex PCR Rapid detection

Atrazine biodegradation efficiency, metabolite detection, and trzD gene expression by enrichment bacterial cultures from agricultural soil

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第12期14卷 1162-1172页

作者： Robinson David Jebakumar SOLOMON Amit KUMAR Velayudhan SATHEEJA SANTHI department of Molecular microbiology School of Biotechnology Madurai Kamaraj University

Atrazine is a selective herbicide used in agricultural fields to control the emergence of broadleaf and grassy weeds. The persistence of this herbicide is influenced by the metabolic action of habituated native microorganisms. This study provides information on the occurrence of atrazine mineralizing bacterial strains with faster metabolizing ability. The enrichment cultures were tested for the biodegradation of atrazine by high-performance liquid chromatography(HPLC) and mass spectrometry. Nine cultures ***01 to ***01 were identified as the degrader of atrazine in the enrichment culture. The three isolates ***01, ***01, and ***01 were identified as efficient atrazine metabolizers. Isolates ***01 and ***01 produced hydroxyatrazine(HA) N-isopropylammelide and cyanuric acid by dealkylation reaction. The isolate ***01 generated deethylatrazine(DEA), deisopropylatrazine(DIA), and cyanuric acid by N-dealkylation in the upper degradation pathway and later it incorporated cyanuric acid in their biomass by the lower degradation pathway. The optimum pH for degrading atrazine by ***01 was 7.0 and 16S rDNA phylogenetic typing identified it as Enterobacter cloacae strain ***01. The highest atrazine mineralization was observed in case of isolate ***01, where an ample amount of trzD mRNA was quantified at 72 h of incubation with atrazine. Atrazine bioremediating isolate E. cloacae strain ***01 could be the better environmental remediator of agricultural soils and the crop fields contaminated with atrazine could be the source of the efficient biodegrading microbial strains for the environmental cleanup process.

关键词： Atrazine Biodegradation Enterobacter cloacae Deethylatrazine Cyanuric acid trzD gene

Bacteriophage secondary infection

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Virologica Sinica 2015年第1期30卷 3-10页

作者： Stephen T Abedon department of microbiology the Ohio State University OH 44906 USA

Phages are credited with having been first described in what we now, officially, are commemorating as the 100 th anniversary of their discovery. Those one-hundred years of phage history have not been lacking in excitement, controversy, and occasional convolution. One such complication is the concept of secondary infection, which can take on multiple forms with myriad consequences. The terms secondary infection and secondary adsorption, for example, can be used almost synonymously to describe virion interaction with already phage-infected bacteria, and which can result in what are described as superinfection exclusion or superinfection immunity. The phrase secondary infection also may be used equivalently to superinfection or coinfection, with each of these terms borrowed from medical microbiology, and can result in genetic exchange between phages, phage-on-phage parasitism, and various partial reductions in phage productivity that have been termed mutual exclusion, partial exclusion, or the depressor effect. Alternatively, and drawing from epidemiology, secondary infection has been used to describe phage population growth as that can occur during active phage therapy as well as upon phage contamination of industrial ferments. Here primary infections represent initial bacterial population exposure to phages while consequent phage replication can lead to additional, that is, secondary infections of what otherwise are not yet phage-infected bacteria. Here I explore the varying meanings and resultant ambiguity that has been associated with the term secondary infection. I suggest in particular that secondary infection, as distinctly different phenomena, can in multiple ways influence the success of phage-mediated biocontrol of bacteria, also known as, phage therapy.

关键词： lysis from without lysis inhibition coinfection parallel secondary infection phage therapy pharmacology serial secondary infection superinfection

Potential Inhibitory Effect of Vitamins Against COVID-19

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Computers, Materials & Continua 2021年第1期66卷 707-714页

作者： Kashaf Junaid Sumera Qasim Humaira Yasmeen Hasan Ejaz Abdullah Alsrhani Muhammad Ikram Ullah Fahad Ahmad Abdul Rehman department of Clinical Laboratory Sciences College of Applied Medical SciencesJouf UniversitySakakaAl JoufSaudi Arabia College of Pharmacy Jouf UniversitySakakaAl JoufSaudi Arabia department of microbiology and Molecular Genetics The Women’s UniversityMultanPakistan department of Computer Sciences Kinnaird College for WomenLahorePakistan department of microbiology and Molecular Genetics University of the PunjabLahorePakistan

Coronavirus disease 2019(COVID-19)is a current pandemic that has affected more than 195 countries *** this severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)pandemic,when treatment strategies are not yet clear and vaccines are not available,vitamins are an excellent choice to protect against this viral *** rationale behind this study was to examine the inhibitory effect of vitamins B,C,and D against the main protease of SARSCoV-2 and angiotensin-converting enzyme 2(ACE2),which have critical rolesin the immune *** docking,performed by using MOE-Dock of the Chemical Computing Group,was used to understand the *** vitamins all docked within the active sites of the Mpro(PDB ID:6LU7)and ACE2 receptor proteins(PDB ID:6VW1).Vitamins B and C delivered maximum energy scores against both targets,while vitamin D displayed a binding energy score of−7.9532 kcal/mol for M^(pro) and−7.9297 for *** efficiency of all three vitamins is higher than the binding energy score of chloroquine(−6.889 kcal/mol),which is now under clinical *** use of vitamins is beneficial,being immune system restorative,and they also act as anti-COVID *** the potential beneficial effects of vitamin B and C are revealed through docking studies,further clinical trials are required for the validation of these results.

关键词： SARS-CoV-2 vitamins docking antivirals chemical computing

Role of activating transcription factor 3 （ATF3） in sublytic C5b-9-induced glomerular mesangial cell apoptosis

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Cellular & Molecular Immunology 2010年第2期7卷 143-151页

作者： Xiaoming Jiang Jing Zhang Mei Xia Wen Qiu Hui Wang Dan Zhao Yingwei Wang department of microbiology and Immunology Nanjing Medical University Nanjing China

Sublytic complement C5b-9 complexes can cause cell apoptosis, but the mechanism of glomerular mesangial cell （GMC） apoptosis mediated by these complexes has not been well defined. The activating transcription factor 3 （ATF3） gene is an immediate early gene for the cell to cope with a variety of stress signals and can promote apoptosis of some cells. In this study, ATF3 expression and cell apoptosis in GMCs induced by sublytic C5b-9 were measured, and then the effects of ATF3 gene over-expression or knockdown on GMC apoptosis induced by sublytic C5b-9 were examined at a fixed time. The results showed that both ATF3 expression and GMC apoptosis were markedly increased and ATF3 over-expression obviously increased sublytic C5b-9-induced GMC apoptosis, whereas ATF3 gene silencing had a significant opposite effect. Collectively, these findings indicate that upregulation of ATF3 gene expression is involved in regulating GMC apoptosis induced by sublytic C5b-9 complexes.

关键词： sublytic C5b-9 apoptosis glomerular mesangial cell (GMC) activating transcription factor 3 (ATF3)

Polyomavirus interaction with the DNA damage response

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Virologica Sinica 2015年第2期30卷 122-129页

作者： Joshua L.Justice Brandy Verhalen Mengxi Jiang department of microbiology University of Alabama at Birmingham Birmingham 35294 USA

Viruses are obligate intracellular parasites that subvert cellular metabolism and pathways to mediate their own replication—normally at the expense of the host cell. Polyomaviruses are a group of small DNA viruses, which have long been studied as a model for eukaryotic DNA replication. Polyomaviruses manipulate host replication proteins, as well as proteins involved in DNA maintenance and repair, to serve as essential cofactors for productive infection. Moreover, evidence suggests that polyomavirus infection poses a unique genotoxic threat to the host cell. In response to any source of DNA damage, cells must initiate an effective DNA damage response(DDR) to maintain genomic integrity, wherein two protein kinases, ataxia telangiectasia mutated(ATM) and ATM- and Rad3-related(ATR), are major regulators of DNA damage recognition and repair. Recent investigation suggests that these essential DDR proteins are required for productive polyomavirus infection. This review will focus on polyomaviruses and their interaction with ATMand ATR-mediated DNA damage responses and the effect of this interaction on host genomic stability.

关键词： polyomavirus DNA damage response genomic instability mitotic stress