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Effect of nitrogen fertilization on yield, N content, and nitrogen fixation of alfalfa and smooth bromegrass grown alone or in mixture in greenhouse pots

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Journal of Integrative Agriculture 2015年第9期14卷 1864-1876页

作者： XIE Kai-yun LI Xiang-lin HE Feng ZHANG Ying-jun WAN Li-qiang David B Hannaway WANG Dong QIN Yan Gamal M A Fadul Institute of animal sciences Chinese Academy of Agricultural Sciences Institute of grassland Science College of Animal Science and TechnologyChina Agricultural University department of Crop and Soil Science Oregon State University

Planting grass and legume mixtures on improved grasslands has the potential advantage of realizing both higher yields and lower environmental pollution by optimizing the balance between applied N fertilizer and the natural process of legume biological nitrogen fixation. However, the optimal level of N fertilization for grass-legume mixtures, to obtain the highest yield, quality, and contribution of N2 fixation, varies with species. A greenhouse pot experiment was conducted to study the temporal dynamics of N2 fixation of alfalfa （Medicago sativa L.） grown alone and in mixture with smooth bromegrass （Bromus inermis Leyss.） in response to the addition of fertilizer N. Three levels of N （0, 75, and 150 kg ha-1） were examined using 15N-labeled urea to evaluate N2 fixation via the 15N isotope dilution method. Treatments were designated NO （0.001 g per pot）, N75 （1.07 g per pot） and N150 （2.14 g per pot）. Alfalfa grown alone did not benefit from the addition of fertilizer N; dry matter was not significantly increased. In contrast, dry weight and N content of smooth bromegrass grown alone was increased significantly by N application. When grown as a mixture, smooth bromegrass biomass was increased significantly by N application, resulted in a decrease in alfalfa biomass. In addition, individual alfalfa plant dry weight （shoots＋roots） was significantly lower in the mixture than when grown alone at all N levels. Smooth bromegrass shoot and root dry weight were significantly higher when grown with alfalfa than when grown alone, regardless of N application level. When grown alone, alfalfa＇s N2 fixation was reduced with N fertilization （R2=0.9376,P=0.0057）. When grown in a mixture with smooth bromegrass, with 75 kg ha-1 of N fertilizer, the percentage of atmospheric N2 fixation contribution to total N in alfalfa （%Ndfa） had a maximum of 84.07 and 83.05% in the 2nd and 3rd harvests, respectively. Total 3-harvest %Ndfa was higher when alfalfa was grown in a mixture than when grow

关键词： alfalfa (Medicago sativa) smooth bromegrass (Bromus inermis) nitrogen (N2) fixation nitrogen partitioning,15N mixture monoculture

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Transcriptome analysis of salt-responsive genes and SSR marker exploration in Carex rigescens using RNA-seq

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Journal of Integrative Agriculture 2018年第1期17卷 184-196页

作者： LI Ming-na LONG Rui-cai FENG Zi-rong LIU Feng-qi SUN Yan ZHANG Kun KANG Jun-mei WANG Zhen CAO Shi-hao grassland Science department College of Animal Science and TechnologyChina Agricultural University Institute of animal Science Chinese Academy of Agricultural Sciences Institute of Pratacultural Science Heilongjiang Academy of Agricultural Sciences

Carex rigescens （Franch.） V. Krecz is a wild turfgrass perennial species in the Carex genus that is widely distributed in salinised areas of northern China. To investigate genome-wide salt-response gene networks in C. rigescens, transcriptome analysis using high-throughput RNA sequencing on C. rigescens exposed to a 0.4% salt treatment （Cr_Salt） was compared to a non-salt control （Cr_Ctrl）. In total, 57 742 546 and 47 063 488 clean reads were obtained from the Cr Ctrl and Cr Salt treatments, respectively. Additionally, 21 954 unigenes were found and annotated using multiple databases. Among these unigenes, 34 were found to respond to salt stress at a statistically significant level with 6 genes up-regulated and 28 downregulated. Specifically, genes encoding an EF-hand domain, ZFP and AP2 were responsive to salt stress, highlighting their roles in future research regarding salt tolerance in C. rigescens and other plants. According to our quantitative RT-PCR results, the expression pattern of all detected differentially expressed genes were consistent with the RNA-seq results. Furthermore, we identified 11 643 simple sequence repeats （SSRs） from the unigenes. A total of 144 amplified successfully in the C. rigescens cultivar LOping 1, and 69 of them reflected polymorphisms between the two genotypes tested. This is the first genome-wide transcriptome study of C. rigescens in both salt-responsive gene investigation and SSR marker exploration. Our results provide further insights into genome annotation, novel gene discovery, molecular breeding and comparative genomics in C. rigescens and related grass species.

关键词： salt stress Carex rigescens transcriptome differentially expressed genes SSR markers

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Attribution of explanatory factors for change in soil organic carbon density in the native grasslands of Inner Mongolia,China

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Journal of Arid Land 2018年第3期10卷 375-387页

作者： JIN Dongyan Phil J MURRAY XIN Xiaoping QIN Yifei CHEN Baorui QING Gele ZHANG Zhao YAN Ruirui Institute of Agricultural Resources and Regional Planning Chinese Academy of Agricultural Sciences department of Sustainable Soils and grassland Systems Rothamsted Research North Wyke department of grassland Ecology and animal Husbandry Xilingol Vocational College

The variation in soil organic carbon density（SOCD） has been widely documented at various spatial and temporal scales. However, an accurate method for examining the attribution of explanatory factors for change in SOCD is still lacking. This study aims to attribute and quantify the key climatic factors, anthropogenic activities, and soil properties associated with SOCD change in the native grasslands of Inner Mongolia, China, by comparing data between the 1960s and the 2010s. In 2007 and 2011, we resampled 142 soil profiles which were originally sampled during 1963–1964 in the native grasslands of Inner Mongolia. SOCD was determined in A horizon（eluvial horizon） of the soil. We selected the explanatory factors based on a random forest method, and explored the relationships between SOCD change and each of the explanatory factors using a linear mixed model. Our results indicated that the change in SOCD varied from the east to the west of Inner Mongolia, and SOCD was 18% lower in the 2010s than in the 1960s. The lower SOCD in the 2010s may primarily be attributed to the increasing in mean annual water surface evaporation, which explained approximately 10% and 50% of the total variation and explainable variation in the change in SOCD, respectively. The sand content of the soil is also a significant explanatory factor for the decrease in SOCD, which explained about 4% and 21% of the total variation and explainable variation in the change in SOCD, respectively. Furthermore, the collection of quantitative information on grazing frequency and duration may also help to improve our understanding of the anthropogenic factors that govern the change in SOCD.

关键词： soil organic carbon climate change soil texture mixed linear model effect isolation native grasslands

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Nitric oxide, g-aminobutyric acid, and mannose pretreatment influence metabolic profiles in white clover under water stress

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Journal of Integrative Plant Biology 2019年第12期61卷 1255-1273页

作者： Zhou Li Bin Yong Bizhen Cheng Xing Wu Yan Zhang Xinquan Zhang Yan Peng department of grassland Science College of Animal Science and Technology Sichuan Agricultural University

Nitric oxide(NO),γ-aminobutyric acid(GABA),and mannose(MAS)could be important regulators of plant growth and adaptation to water *** application of sodium nitroprusside(SNP,a NO donor),GABA,and MAS improved plant growth under watersufficient conditions and effectively mitigated water stress damage to white *** metabonomic analysis showed that both SNP and GABA application resulted in a significant increase in myo-inositol content;the accumulation of mannose was commonly regulated by SNP and MAS;GABA and MAS induced the accumulation of aspartic acid,quinic acid,trehalose,and glycerol under water *** addition,citric acid was uniquely up-regulated by SNP associated with tricarboxylic acid(TCA)cycle under water *** induced the accumulation of GABA,glycine,methionine,and aconitic acid related to GABA shunt,amino acids metabolism,and TCA cycle in response to water *** uniquely enhanced the accumulation of asparagine,galactose,and D-pinitol in association with amino acids and sugars metabolism under water ***-,GABA-,and MAS-induced changes of metabolic profiles and associated metabolic pathways could contribute to enhanced stress tolerance via involvement in the TCA cycle for energy supply,osmotic adjustment,antioxidant defense,and signal transduction for stress defense in white clover.

关键词： uniquely donor sugar

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Transcriptional changes in mesenteric and subcutaneous adipose tissue from Holstein cows in response to plane of dietary energy

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Journal of animal Science and Biotechnology 2018年第1期9卷 233-247页

作者： S.J.Moisá P.Ji J.K.Drackley S.L.Rodriguez-Zas J.J.Loor department of animal sciences Auburn University department of animal sciences University of Illinois

Background： Dairy cows can readily overconsume dietary energy during most of the prepartum period, often leading to higher prepartal concentrations of insulin and glucose and excessive body fat deposition. The end result of these physiologic changes is greater adipose tissue lipolysis post-partum coupled with excessive hepatic lipid accumulation and compromised health. Although transcriptional regulation of the adipose response to energy availability is well established in non-ruminants, such regulation in cow adipose tissue depots remains poorly ***： Effects of ad-libitum access to high [HIGH; 1.62 Mcal/kg of dry matter（DM）] or adequate（CON; 1.35 Mcal/kg of DM） dietary energy for 8 wk on mesenteric（MAT） and subcutaneous（SAT） adipose tissue transcript profiles were assessed in non-pregnant non-lactating Holstein dairy cows using a 13,000-sequence annotated bovine oligonucleotide microarray. Statistical analysis revealed 409 and 310 differentially expressed genes（DEG） due to tissue and diet. Bioinformatics analysis was conducted using the Dynamic Impact Approach（DIA） with the KEGG pathway database. Compared with SAT, MAT had more active biological processes related to adipose tissue accumulation（adiponectin secretion） and signs of pro-inflammatory processes due to adipose tissue expansion and macrophage infiltration（generation of ceramides）. Feeding the HIGH diet led to changes in m RNA expression of genes associated with cell hypertrophy（regucalcin）, activation of adipogenesis（phospholipid phosphatase 1）,insulin signaling activation（neuraminidase 1） and angiogenesis（semaphorin 4 G, plexin B1）. Further, inflammation due to HIGH was underscored by m RNA expression changes associated with oxidative stress response（coenzyme Q3, methyltransferase）, ceramide synthesis（N-acylsphingosine amidohydrolase 1）, and insulin signaling（interferon regulatory factor 1, phosphoinositide-3-kinase regulatory subunit 1, retinoic acid receptor alpha）. Activation of ribos

关键词： Adipose tissue Dairy cow Dietary energy Transcriptome

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Stress and immunological response of heifers divergently ranked for residual feed intake following an adrenocorticotropic hormone challenge

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Journal of animal Science and Biotechnology 2018年第1期9卷 111-118页

作者： A.K.Kelly P.Lawrence B.Earley D.A.Kenny M.McGee School of Agriculture and Food Science College of Health and Agricultural SciencesUniversity College Dublin Livestock Systems Research department Teagasc Animal&Grassland Research and Innovation CentreGrangeDunsanyCo. animal and Bioscience Research department Animal&Grassland Research and Innovation CentreTeagascDunsanyCo.

Background： When an animal is exposed to a stressor, metabolic rate, energy consumption and utilisation increase primarily through activation of the hypothalamic-pituitary-adrenal（HPA） axis. Changes to partitioning of energy by an animal are likely to influence the efficiency with which it is utilised. Therefore, this study aimed to determine the physiological stress response to an exogenous adrenocorticotropic hormone（ACTH） challenge in beef heifers divergently ranked on phenotypic residual feed intake（RFI）.Results： Data were collected on 34 Simmental weaning beef heifers the progeny of a well characterized and divergently bred RFI suckler beef herd. Residual feed intake was determined on each animal during the postweaning stage over a 91-day feed intake measurement period during which they were individually offered adlibitum grass silage and 2 kg of concentrate per head once daily. The 12 highest [0.34 kg DM/d] and 12 lowest[-0.48 kg DM/d] ranking animals on RFI were selected for use in this study. For the physiological stress challenge heifers（mean age 605 ± 13 d; mean BW 518 ± 31.4 kg） were fitted aseptically with indwelling jugular catheters to facilitate intensive blood collection. The response of the adrenal cortex to a standardised dose of ACTH（1.98 IU/kg metabolic BW^（0.75）） was examined. Serial blood samples were analysed for plasma cortisol, ACTH and haematology variables. Heifers differing in RFI did not differ（P = 0.59） in ACTH concentrations. Concentration of ACTH peaked（P 〈 0.001） in both RFI groups at 20 min post-ACTH administration, following which concentration declined to baseline levels by 150 min. Similarly, cortisol systemic profile peaked at 60 min and concentrations remained continuously elevated for 150 min. A RFI × time interaction was detected for cortisol concentrations（P = 0.06） with high RFI heifers had a greater cortisol response than Low RFI from 40 min to 150 min relative to ACTH administration. Cortisol response was positively assoc

关键词： Beef cattle Cortisol Feed efficiency Residual feed intake Stress response

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Effect of genotype on duodenal expression of nutrient transporter genes in dairy cows

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Journal of animal Science and Biotechnology 2014年第2期5卷 145-153页

作者： Sinéad M Waters Kate Keogh Frank Buckley David A Kenny Teagasc animal and Bioscience Research department Animal and Grassland Research and Innovation CentreGrangeDunsanyCo.MeathIreland Teagasc animal and Bioscience Research department Animal and Grassland Research and Innovation Centre MooreparkFermoyCo CorkIreland

Background： Studies have shown clear differences between dairy breeds in their feed intake and production efficiencies. The duodenum is critical in the coordination of digestion and absorption of nutrients. This study examined gene transcript abundance of important classes of nutrient transporters in the duodenum of non lactating dairy cows of different feed efficiency potential, namely Holstein-Friesian （HF）, Jersey （JE） and their F1 hybrid. Duodenal epithelial tissue was collected at slaughter and stored at -80℃. Total RNA was extracted from tissue and reverse transcribed to generate cDNA. Gene expression of the following transporters, namely nucleoside amino acid; sugar; mineral; and lipid transporters was measured using quantitative real-time RT-PCR. Data were statistically analysed using mixed models ANOVA in SAS. Orthogonal contrasts were used to test for potential heterotic effects and spearman correlation coefficients calculated to determine potential associations amongst gen, expression values and production efficiency variables. Results： While there were no direct effects of genotype on expression values for any of the genes examined, there was evidence for a heterotic effect （P 〈 0.05） on ABCGS, in the form of increased expression in the F1 genotype compared to either of the two parent breeds. Additionally, a tendency for increased expression of the amino acid transporters, SLC3A1 （P= 0.072）, SLC3A2 （P= 0.081） and SLC6A 14 （P= 0.072） was also evident in the F1 genotype. A negative （P 〈 0.05） association was identified between the expression of the glucose transporter gene SLCSA1 and total lactational milk solids yield, corrected for body weight. Positive correlations （P 〈 0.05） were also observed between the expression values of genes involved in common transporter roles. Conclusion： This study suggests that differences in the expression of sterol and amino acid transporters in the duodenum could contribute towards the documented differences in feed effic

关键词： Bovine Duodenum Gene expression Nutrient transporters

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Process stability and microbial community composition in pig manure and food waste anaerobic co-digesters operated at low HRTs

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Frontiers of Environmental Science & Engineering 2017年第3期11卷 53-65页

作者： Conor Dennehy Peadar G. Lawlor Gillian E. Gardiner Yan Jiang Paul Cormican Matthew S. McCabe Xinmin Zhan department of Civil Engineering College of Engineering and Informatics National University of Ireland Galway Ireland Teagasc Pig Development department Animal and Grassland Research and Innovation Centre Fermoy Ireland department of Science Waterford Institute of Technology Waterford Ireland Teagasc animal and Bioscience Research department Animal and Grassland Research and Innovation Centre Dunsany Meath Ireland

This study assessed the effects of reducing hydraulic retention times （HRTs） from 21 days to 10.5 days when anaerobically co-digesting pig manure and food waste. Continuously stirred tank reactors of 3.75 L working volume were operated in triplicate at 42℃. Digester HRT was progressively decreased from 21 to 15 days to 10.5 days, with an associated increase in organic loading rate （OLR） from 3.1 kg volatile solids （VS）·m^-***^-1 to 5.1 kg VS·m^***-1 to 7.25 kg VS.m^-3·day^-1. Reducing HRT from 21 days to 15 days caused a decrease in specific methane yields and VS removal rates. Operation at a HRT of 10.5 days initially resulted in the accumulation of isobutyric acid in each reactor. High throughput 16S rRNA gene sequencing revealed that this increase coincided with a shift in acidogenic bacterial populations, which most likely resulted in the increased isobutyric acid concentrations. This may in turn have caused the increase in relative abundance of Clocamonaceae bacteria, which syntrophically degrade non-acetate volatile fatty acids （VFAs） into H2 and CO2. This, along with the increase in abundance of other syntrophic VFA oxidizers, such as Spiorchatetes, suggests that VFA oxidation plays a role in digester operation at low HRTs. Reducing the HRT to below 21 days compromised the ability of the anaerobic digestion system to reduce enteric indicator organism counts below regulatory limits.

关键词： Biogas Sequencing Clocamonaeeae Spiorchatetes Isobutyrate Biosafety

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Effects of neutral salt and alkali on ion distributions in the roots,shoots,and leaves of two alfalfa cultivars with differing degrees of salt tolerance

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Journal of Integrative Agriculture 2017年第8期16卷 1800-1807页

作者： WANG Xiao-shan REN Hai-long WEI Zen-wu WANG Yun-wen REN Wei-bo department of grassland Science College of Animal Science and TechnologyYangzhou UniversityYangzhou 225009P.R.China Institute of grassland Research Chinese Academy of Agricultural SciencesHohhot 010000P.R.China department of grassland Science College of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193P.R.China

The effects of neutral salt and alkali on the ion distribution were investigated in two alfalfa （Medicago sativa L.） cultivars, including Zhongmu 1, a high salt-tolerant cultivar, and Algonquin, a low salt-tolerant cultivar. The alkali stress expressed more serious growth inhibition than the neutral salt stress at the same Na＋ concentration. Compared with Algonquin, Zhongmu 1 did not exhibit a higher alkali tolerance under the Na2CO3-NaHCO3 treatment with the low Na＋ concentration （50 mmol L-l）. The alkali increased the accumulation of Na＋, Ca2＋, and Mg2＋ in the root and changed the Ca2＋ and Mg2＋ balance in the entire alfalfa plant. The salt and alkali stresses decreased the K＋ and Fe3＋ contents of the roots and leaves, the root Mn2＋ content, and the shoot Zn2＋ content, but they increased the Fe3＋ accumulation of the shoots, the shoot and leaf Cu2＋ contents, and the leaf Zn2＋ content in both alfalfa cultivars. Based on the results obtained under the conditions of this experiment, we found that the salt and alkali stresses reduced the plant growth in both alfalfa cultivars, while the alkali caused a stronger stress than the neutral salt in alfalfa. Thus, we conclude that under hydroponic conditions, the deleterious effects of the alkali on plants are due to the distribution change of some trophic ion balance in the roots, shoots, and leaves of the plants by causing of Na＋, CO3^2-, and/or HCO3- stresses.

关键词： alfalfa ion distribution neutral salts alkali stress

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Non-antibiotic feed additives in diets for pigs:A review

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animal Nutrition 2018年第2期4卷 113-125页

作者： Yanhong Liu Charmaine D.Espinosa Jerubella J.Abelilla Gloria A.Casas L.Vanessa Lagos Su A.Lee Woong B.Kwon John K.Mathai Diego M.D.L.Navarro Neil W.Jaworski Hans H.Stein department of animal Science University of California department of animal sciences University of Illinois department of animal Production College of Animal and Veterinary SciencesUniversity of Colombia Trouw Nutrition

A number of feed additives are marketed to assist in boosting the pigs' immune system, regulate gut microbiota, and reduce negative impacts of weaning and other environmental *** most commonly used feed additives include acidifiers, zinc and copper, prebiotics, direct-fed microbials, yeast products, nucleotides, and plant *** of pharmacological levels of zinc and copper, certain acidifiers, and several plant extracts have been reported to result in improved pig performance or improved immune function of *** is also possible that use of prebiotics, direct-fed microbials, yeast,and nucleotides may have positive impacts on pig performance, but results have been less consistent and there is a need for more research in this area.

关键词： Acidifiers Direct-fed microbials Minerals Plant extracts Prebiotics Pigs

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