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Hepa.i. steatosis is associated with dysregulated cholesterol metabolism and altered protein acetyla.i.n dyna.i.s in chickens

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Journal of animal Science and Biotechnology 2024年第1期15卷 53-67页

作者： Xiaoli Guo Qianqian Zhou Jia.i.g Jin Fangren Lan Chaoliang Wen Junying Li Ning Yang Congjiao Sun Na.i.nal Engineering Laboratory for animal Breeding and Key Laboratory of animal Genetics Breeding and ReproductionMinistry of Agriculture and Rural AffairsChina Agricultural UniversityBeijing 100193China

Background Hepa.i. steatosis is a prevalent ma.i.esta.i.n of fatty liver, that has detrimental effect on the health and productivity of la.i.g hens, resulting in economic losses to the poultry industry. Here, we aimed to systema.i.ally investigate the genetic regulatory mecha.i.ms of hepa.i. steatosis in la.i.g hens.Methods Ninety individuals with the most prominent characteristics were selected from 686 la.i.g hens according to the accumula.i.n of lipid droplets in the liver, and were graded into three groups, including the control, mild hepa.i. steatosis and severe hepa.i. steatosis groups. A combina.i.n of transcriptome, proteome, acetylome and lipidome analyses, along with bioinforma.i.s analysis were used to screen the key biological processes, modifica.i.ns and lipids associated with hepa.i. steatosis.Results The ra.i.na.i.y of the hepa.i. steatosis grouping was verified through liver biochemical assays and RNA-seq. Hepa.i. steatosis was characterized by increased lipid deposition and multiple metabolic abnorma.i.ies. integra.i.n of proteome and acetylome revealed that differentially expressed proteins(DEPs) interacted with differentially acetylated proteins(DAPs) and were involved in maintaining the metabolic balance in the liver. Acetyla.i.n altera.i.ns mainly occurred in the progression from mild to severe hepa.i. steatosis, i.e., the enzymes in the fatty a.i. oxida.i.n and bile a.i. synthesis pathways were significantly less acetylated in severe hepa.i. steatosis group than that in mild group(P ipidomics detected a va.i.ty of sphingolipids(SPs) and glycerophospholipids(GPs) were nega.i.ely correlated with hepa.i. steatosis(r ≤-0.5, P ity of hepa.i. steatosis was associated with a decrease in cholesterol and bile a.i. synthesis and an increase in exogenous cholesterol transport.Conclusions in addition to acquiring a global and thorough picture of hepa.i. steatosis in la.i.g hens, we were able to reveal the role of acetyla.i.n in hepa.i. steatosis and depict the ch

关键词： Acetyla.i.n Cholesterol metabolism Hepa.i. steatosis La.i.g hens Multiomics

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RNA m6A dyna.i. modifica.i.n mediated by nucleusloca.i.ed FTO is involved in follicular reserve

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Zoological Research 2024年第2期45卷 415-428页

作者： Jiao-Na Zhang Rui-Ting Wang Francesca-Gioia Klinger Shun-Feng Cheng Wei Shen Xiao-Feng Sun College of Life Sciences Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of ShandongQingdao Agricultural UniversityQingdaoShandong 266109China department of Biomedicine and Prevention University of Rome Tor VergataRome 00133Italy

in eukaryotic orga.i.ms,the most common internal modifica.i.n of messenger RNA(m RNa.i. N6-methyladenosine(m6A).This modifica.i.n can be dyna.i.ally and reversibly controlled by specific enzymes known as m6A writers and erasers.The fat-mass and obesity-associated protein(FTO)catalyzes RNA demethyla.i.n and plays a critical role in va.i.us physiological and pathological processes.Our research identified dyna.i. altera.i.ns in both m6A and FTO during the assembly of primordial follicles,with an inverse rela.i.nship observed for m6A levels and nuclear-loca.i.ed FTO expression.Applica.i.n of Fto small interfering RNA(si RNA)altered the expression of genes related to cell prolifera.i.n,hormone regula.i.n,and cell chemota.i.,and affected RNA alterna.i.e splicing.Overexpression of the full-length Fto gene led to changes in m6A levels,alterna.i.e splicing of Cdk5,cell prolifera.i.n,cell cycle progression,and proportion of primordial follicles.Conversely,overexpression of Fto lacking a nuclear loca.i.a.i.n signal(NLS)did not significantly alter m6A levels or primordial follicle assembly.These findings suggest that FTO,loca.i.ed in the nucleus but not in the cytoplasm,regulates RNA m6A demethyla.i.n and plays a role in cell prolifera.i.n,cell cycle progression,and primordial follicle assembly.These results highlight the potential of m6A and its eraser FTO as possible biomarkers and therapeutic targets.

关键词： m6A FTO Ovary Primordial follicle assembly Alterna.i.e splicing

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Chroma.i. condensa.i.n but not DNa.i.tegrity of pig sperm is greater in the sperm-rich fraction

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Journal of animal Science and Biotechnology 2024年第1期15卷 171-181页

作者： Estel Viñolas-Vergés Jordi Ribas-Maynou isabel Barranco Ca.i.a Peres Rubio Sergi Bonet Jordi Roca Marc Yeste Unit of Cell Biology Department of BiologyFaculty of SciencesUniversity of GironaGironaSpain Biotechnology of animal and Human reproduction(TechnoSperm) Institute of Food and Agricultural TechnologyUniversity of GironaGironaSpain department of Medicine and animal Surgery Faculty of Veterinary ScienceUniversity of MurciaMurciaSpain Catalan institution for Research and Advanced Studies(iCREA) S08010 BarcelonaSpain

Background Prota.i.a.i.n and condensa.i.n of sperm chroma.i. as well as DNa.i.tegrity play an essential role during fertiliza.i.n and embryo development.in some mammals,like pigs,ejaculates are emitted in three separate fractions:pre-sperm,sperm-rich(SRF)and post sperm-rich(PSRF).These fractions are known to vary in volume,sperm concentra.i.n and qua.i.y,as well as in the origin and composition of seminal plasma(SP),with differences being also observed within the SRF one.Yet,whether dispa.i.ies in the DNa.i.tegrity and chroma.i. condensa.i.n and pro-ta.i.a.i.n of their sperm exist has not been interrogated.Results This study determined chroma.i. prota.i.a.i.n(Chromomycin A3 test,CMA_(3)),condensa.i.n(Dibromobi-mane test,DBB),and DNa.i.tegrity(Comet assay)in the pig sperm contained in the first 10 m L of the SRF(SRF-P1),the remaining portion of the sperm-rich fraction(SRF-P2),and the post sperm-rich fraction(PSRF).While chroma.i. prota.i.a.i.n was found to be similar between the different ejaculate fractions(P>0.05),chroma.i. condensa.i.n was seen to be greater in SRF-P1 and SRF-P2 than in the PSRF(P=0.018 and P=0.004,respectively).Regarding DNa.i.tegrity,no differences between fractions were observed(P>0.05).As the SRF-P1 has the highest sperm concentra-tion and ejaculate fractions are known to differ in antioxidant composition,the oxida.i.e stress index(OSi)in SP,calcu-lated as total oxidant activity divided by total antioxidant capa.i.y,was tested and confirmed to be higher in the SRF-P1 than in SRF-P2 and PSRF(0.42±0.06 vs.0.23±0.09 and 0.08±0.00,respectively;Pis index,in addition,was observed to be correlated to the sperm concentra.i.n of each fraction(Rs=0.973;Pion While sperm DNa.i.tegrity was not found to differ between ejaculate fractions,SRF-P1 and SRF-P2 were observed to exhibit greater chroma.i. condensa.i.n than the PSRF.This could be related to the OSi of each fraction.

关键词： Chroma.i. Condensa.i.n DNa.i.tegrity Ejaculate fractions Pig Prota.i.a.i.n Sperm

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Fermented calcium butyrate supplementa.i.n in post-peak la.i.g hens improved ova.i.n function and tibia qua.i.y through the“gut-bone” a.i.

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animal Nutrition 2024年第1期16卷 350-362页

作者： Huaiyong Zhang Yongshuai Wang Yilu Wang Bin Wei Leilei Wang Minh Tu Nguyen Xiangyun Lv Yanqun Huang Wen Chen College of animal Science and Technology Key Laboratory of Animal Biochemistry and NutritionMinistry of AgricultureHenan Agricultural University Laboratory for animal Nutrition and animal Product Qua.i.y Department of Animal Sciences and Aquatic EcologyGhent University department of Agriculture and Forestry Hue University Charoen Pokphand Group Co. Ltd.

The compromised egg qua.i.y and leg abnorma.i.y during the end of the la.i.g cycle(after 40 weeks)have been lea.i.g to poor animal welfare and substantial economic losses. Therefore, the effects of fermented calcium(Ca) butyrate, produced by fermenta.i.n by Clostridium butyricum, on production,eggshell qua.i.y, and tibial property of hens were explored. A total of 192 Hy-line brown la.i.g hens at50-week-old were assigned to a basal diet or the basal diet with 300 mg/kg of the fermented Ca butyrate from 50 to 58 weeks of age. Each treatment had 6 replicates with 16 hens each. The diet supplemented with 300 mg/kg fermented Ca butyrate notably increased egg weight, ova.i.n follicle number, and eggshell strength(P = 0.072) as compared to the basal diet, which were associated with cytokine secretion, toll-like receptor signa.i.g pathways, and intestinal immunity based on the RNA-seq data from the granulosa. Dietary Ca butyrate inclusion decreased the expression of ileal tumor necrosis factor-alpha and serum pro-inflammatory cytokine concentra.i.n, as well as increased the content of serum immunoglobulin A when compared to the basal diet(both P irds that received fermented Ca butyrate diets exhibited higher villus height(P ion of tight junction proteins, whereas it did not alter the composition of cecal microbiota(P > 0.05). in addition, the diet with fermented Ca butyrate reduced the number of osteoclasts in the proximal tibia and the level of C-terminal cross-linked telopeptide of type i collagen, a bone resorption marker(P it tended to increase the concentra.i.n of the procollagen type i N-terminal propeptide that reflects bone forma.i.n marker in serum. Moreover, the layers fed fermented Ca butyrate diets possessed higher(P imal tibia, yield load, and ultimate load than those that consumed basal diets. Collectively, dietary fermented Ca butyrate supplementa.i.n in post-peak layer diets improved the ovari

关键词： Fermented Ca butyrate Ova.i.n property Tibia metabolism Layer

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Therapeutic advances in atrial fibrilla.i.n based on animal models

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2024年第2期25卷 135-152页

作者： Qian GONG Xuan LE Pengcheng YU Lenan ZHUANG institute of Genetics and reproduction College of Animal SciencesZhejiang UniversityHangzhou310058China Key Laboratory of Cardiovascular intervention and Regenera.i.e Medicine of Zhejiang Province Department of CardiologySir Run Run Shaw HospitalSchool of MedicineZhejiang UniversityHangzhou310016China

Atrial fibrilla.i.n(AF)is the most prevalent sustained cardiac arrhythmia among humans,with its incidence increa.i.g significantly with age.Despite the high frequency of AF in clinical practice,its etiology and management remain elusive.To develop effective treatment strategies,it is impera.i.e to comprehend the underlying mecha.i.ms of AF;therefore,the establishment of animal models of AF is vital to explore its pathogenesis.While spontaneous AF is rare in most animal species,several large animal models,particularly those of pigs,dogs,and horses,have proven as invaluable in recent years in advancing our knowledge of AF pathogenesis and developing novel therapeutic options.This review aims to provide a comprehensive discussion of va.i.us animal models of AF,with an empha.i. on the unique features of each model and its utility in AF research and treatment.The data summa.i.ed in this review provide valuable insights into the mecha.i.ms of AF and can be used to evaluate the efficacy and safety of novel therapeutic interventions.

关键词： Atrial fibrilla.i.n animal model Therapeutic

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Polyoxidovanadates a new therapeutic alterna.i.e for neurodegenera.i.e and a.i.g diseases

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Neural Regenera.i.n Research 2024年第3期19卷 571-577页

作者： Sonia.i.ais Gonzalez-Cano Gonzalo Flores Jorge Guevara Julio Cesar Morales-Medina Samuel Treviño Alfonso Diaz Faculty of Chemical Sciences Benemerita Autonomous University of PueblaPueblaMexico institute of Physiology Benemerita Autonomous University of PueblaPueblaMexico department of Biochemistry Faculty of MedicineNational Autonomous University of MexicoMexico CityMexico animal reproduction Research Center CINVESTAV-Autonomous University of TlaxcalaTlaxcalaMexico

a.i.g is a natural phenomenon characterized by a progressive decline in physiological integrity,lea.i.g to a deteriora.i.n of cognitive function and increa.i.g the risk of suffering from chronic-degenera.i.e diseases,including cardiovascular diseases,osteoporosis,cancer,diabetes,and neurodegenera.i.n.a.i.g is considered the major risk factor for Parkinson’s and Alzheimer’s disease develops.Likewise,diabetes and insulin resistance constitute additional risk factors for developing neurodegenera.i.e disorders.Currently,no treatment can effectively reverse these neurodegenera.i.e pathologies.However,some antidiabetic drugs have opened the possibility of being used against neurodegenera.i.e processes.in the previous framework,Vana.i.m species have demonstrated a notable antidiabetic effect.Our research group evaluated polyoxidovanadates such as decavanadate and metforminium-decavanadate with preventive and corrective activity on neurodegenera.i.n in brain-specific areas from rats with metabolic syndrome.The results suggest that these polyoxidovanadates induce neuronal and cognitive restora.i.n mecha.i.ms.This review aims to describe the therapeutic potential of polyoxidovanadates as insulin-enhancer agents in the brain,constituting a therapeutic alterna.i.e for a.i.g and neurodegenera.i.e diseases.

关键词： Alzheimer’s disease antidiabetic brain cognition diabetes insulin neurodegenera.i.n neuroinflamma.i.n oxida.i.e stress Vana.i.m species

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DNA methyla.i.n in poultry:a review

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Journal of animal Science and Biotechnology 2024年第2期15卷 509-518页

作者： Xing Ju Zhijun Wang Danfeng Cai Semiu Fola.i.i Bello Qinghua Nie State Key Laboratory for Conserva.i.n and Utiliza.i.n of Subtropical Agro‑Bioresources Lingnan Guangdong Laboratory of AgricultureCollege of Animal ScienceSouth China Agricultural UniversityGuangzhouGuangdong 510642China Guangdong Provincial Key Lab of Agro‑animal Genomics and Molecular Breeding and Key Lab of Chicken Genetics Breeding and ReproductionMinistry of AgricultureGuangzhouGuangdong 510642China College of animal Science and Technology Zhejiang Agriculture and Forestry University666 Wusu RoadLin’an 311300China

As an important epigenetic modifica.i.n,DNA methyla.i.n is involved in many biological processes such as animal cell differentia.i.n,embryonic development,genomic imprinting and sex chromosome inactiva.i.n.As DNA methyla.i.n sequencing becomes more sophisticated,it becomes possible to use it to solve more zoological problems.This paper reviews the characteristics of DNA methyla.i.n,with empha.i. on the research and applica.i.n of DNA methyla.i.n in poultry.

关键词： CpG islands Differentially methylated genes Differentially methylated regions DNA methyla.i.n Poultry

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Vita.i. A regulates mitochondrial biogenesis and function through p38 MAPK‑PGC‑1α signa.i.g pathway and alters the muscle fiber composition of sheep

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Journal of animal Science and Biotechnology 2024年第2期15卷 898-910页

作者： Pengkang Song Jia.i. Zhao Fanqinyu Li Xiaoyi Zhao Jinxin Feng Yuan Su Bo Wang Junxing Zhao College of animal Science Shanxi Agricultural UniversityTaiguShanxi 030801People’s Republic of China State Key Laboratory of animal Nutrition and Feeding Department of Animal Nutrition and Feed ScienceCollege of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193People’s Republic of China

Background Vita.i. A(VA)and its metabolite,retinoic a.i.(RA),are of great interest for their wide range of physiological functions.However,the regulatory contribution of VA to mitochondrial and muscle fiber composition in sheep has not been reported.Method Lambs were injected with 0(control)or 7,500 iU VA palmitate into the biceps femoris muscle on d 2 after birth.At the age of 3 and 32 weeks,longissimus dorsi(LD)muscle samples were obtained to explore the effect of VA on myofiber type composition.in vitro,we investigated the effects of RA on myofiber type composition and intrinsic mecha.i.ms.Results The proportion of type i myofiber was greatly increased in VA-treated sheep in LD muscle at harvest.VA greatly promoted mitochondrial biogenesis and function in LD muscle of sheep.Further explora.i.n revealed that VA elevated PGC-1αmRNA and protein contents,and enhanced the level of p38 MAPK phosphoryla.i.n in LD muscle of sheep.in addition,the number of type i myofibers with RA treatment was significantly increased,and type iix myofibers was significantly decreased in primary myoblasts.Consistent with in vivo experiment,RA significantly improved mitochondrial biogenesis and function in primary myoblasts of sheep.We then used si-PGC-1αto inhibit PGC-1αexpression and found that si-PGC-1αsignificantly abrogated Ra.i.duced the forma.i.n of type i myofibers,mitochondrial biogenesis,MitoTracker staining intensity,UQCRC1 and ATP5A1 expression,SDH activity,and enhanced the level of type iix muscle fibers.These data suggested that Ra.i.proved mitochondrial biogenesis and function by promoting PGC-1αexpression,and increased type i myofibers.in order to prove that the effect of RA on the level of PGC-1αis caused by p38 MAPK signa.i.g,we inhibited the p38 MAPK signa.i.g using a p38 MAPK inhibitor,which significantly reduced Ra.i.duced PGC-1αand MyHC i levels.Conclusion VA promoted PGC-1αexpression through the p38 MAPK signa.i.g pathway,improved mitochondrial biogenesis,and altered the composition of muscle fiber type.

关键词： Mitochondria Muscle fiber type PGC-1α p38 MAPK Retinoic a.i. Vita.i. A

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Pre‑hatch thermal ma.i.ula.i.n of embryos and post‑hatch baicalein supplementa.i.n mitigated heat stress in broiler chickens

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Journal of animal Science and Biotechnology 2024年第3期15卷 1071-1085页

作者： Sa.i. Al Amaz Ajay Chaudhary Prem Lal Mahato Rajesh Jha Birendra Mishra department of Human Nutrition Food and Animal SciencesCollege of Tropical Agriculture and Human ResourcesUniversity of Hawai’i at ManoaAgSci 2161955 East‑West RdHonoluluHI 96822USA

Background High environmental temperatures induce heat stress in broiler chickens,affecting their health and pro-duction performance.Several dietary,managerial,and genetics strategies have been tested with some success in mitiga.i.g heat stress(HS)in broilers.Developing novel HS mitiga.i.n strategies for sustaining broiler production is critically needed.This study investigated the effects of pre-hatch thermal ma.i.ula.i.n(TM)and post-hatch baica-lein supplementa.i.n on growth performance and health parameters in heat-stressed broilers.Results Six hundred fertile Cobb 500 eggs were incubated for 21 d.After candling on embryonic day(ED)10,238 eggs were thermally ma.i.ulated at 38.5℃ with 55%rela.i.e humidity(RH)from ED 12 to 18,then transferred to the hatcher(ED 19 to 21,standard temperature)and 236 eggs were incubated at a controlled temperature(37.5℃)till hatch.After hatch,180-day-old chicks from both groups were raised in 36 pens(n=10 birds/pen,6 replicates per treatment).The treatments were:1)Control,2)TM,3)control heat stress(CHS),4)thermal ma.i.ula.i.n heat stress(TMHS),5)control heat stress supplement(CHSS),and 6)thermal ma.i.ula.i.n heat stress supplement(TMHSS).All birds were raised under the standard environment for 21 d,followed by chronic heat stress from d 22 to 35(32–33℃ for 8 h)in the CHS,TMHS,CHSS,and TMHSS groups.A thermoneutral(22–24℃)environment was maintained in the Control and TM groups.RH was constant(50%±5%)throughout the trial.All the data were analyzed using one-way ANOVa.i. R and GraphPad software at Pignificantly decreased(Pinal body weight and ADG in CHS and TMHS groups compared to the other groups.Embryonic TM significantly increased(Pion of heat shock protein-related genes(HSP70,HSP90,and HSPH1)and antioxidant-related genes(GPX1 and TXN).TMHS birds showed a significant increment(Pin total cecal vola.i.e fatty a.i.(VFA)concentra.i.n compared to the CHS birds.The cecal microbial analysis showed significan

关键词： Baicalein Growth performance Gut microbiota Heat stress Thermal ma.i.ula.i.n

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Short neuropeptide F in integrated insect physiology

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2024年第5期25卷 389-409页

作者： Marcin CHOLEWiNSKi Szymon CHOWANSKi Jan LUBAWY Arka.i.sz URBANSKi Karolina WALKOWiAK-NOWiCKA Pawet MARCiNiAK department of animal Physiology and Developmental Biology Addm Mickiewicz UniversityPoznan 61-614Poland

The short neuropeptide F(sNPF)fa.i.y of peptides is a multifunctional group of neurohormones involved in the regula.i.n of va.i.us physiological processes in insects.They have been found in a broad spectrum of species,but the number of isoforms in the precursor molecule va.i.s from one to four.The receptor for sNPF(sNPFR),which belongs to the G protein-coupled receptor fa.i.y,has been characterized in va.i.us insect orders and was shown to be an ortholog of the mamma.i.n prolactin-relea.i.g peptide receptor(PrPR).The sNPF signa.i.g pathway interacts with other neurohormones such as insulin-like peptides,SiFa.i.e,and pigment-dispersing factors(PDFs)to regulate va.i.us processes.The main physiological function of sNPF seems to be involved in the regula.i.n of feeding,but the observed effects are species-specific.sNPF is also connected with the regula.i.n of fora.i.g beha.i.r and the olfactory system.The influence of sNPF on feeding and thus energy metabolism may also indirectly affect other vital processes,such as reproduction and development.in addition,these neurohormones are involved in the regula.i.n of locomotor activity and circa.i.n rhythm in insects.This review summa.i.es the current state of knowledge about the sNPF system in insects.

关键词： insect neuropeptide invertebrate neurobiology Short neuropeptide F(sNPF) Feeding Neurohormone

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