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Journal of Systems science and Systems Engineering 2024年第2期33卷 187-206页

作者： Mintu Kumar Himani Pant S.B.Singh department of Mathematics Statistics and Computer science G.B.Pant University of AgricultureTechnologyPantnagarUttarakhandIndia

Uncertainty is an important factor that needs to be considered while analyzing the performance of any engineering *** order to quantify uncertainty,fuzzy set theory is frequently used by most of researchers,including energy system *** to the classical reliability theory,component lifetimes have crisp parameters,but due to uncertainty and inaccuracy in data,it is sometimes very difficult to determine the exact values of these parameters in real-world *** overcome this difficulty in the current research,failure and repair rates were taken as triangular fuzzy numbers to determine the fuzzy availability of a system undergoing calendar-based periodic inspection subject to multiple failure modes(FMs).It was assumed that each component in the system had an exponential failure rate and repair rate with fuzzy *** FMs were explicitly taken into account when a functional state of the system was *** FM had a random failure *** the occurrence of any failure,a random time was selected for the relevant corrective repair *** proposed research was studied for one of the major sources of green energy,namely a wind turbine system wherein all the derived propositions have been implemented on it.

关键词： Fuzzy reliability fuzzy availability wind turbine system multiple failure modes triangular fuzzy number

Changes in climatic conditions drive variations in arbuscular mycorrhizal fungi diversity and composition in semi-arid oak forests

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Journal of Forestry Research 2024年第5期35卷 106-119页

作者： Nahid Jafarian Javad Mirzaei Reza Omidipour Yahya Kooch department of Forest science Faculty of AgricultureIlam UniversityIlam 69315-516Iran department of Range and Watershed Management Faculty of AgricultureIlam UniversityIlam 69315-516Iran Faculty of Natural Resources and Marine sciences Tarbiat Modares UniversityNoor 46417-76489MazandaranIran

Arbuscular mycorrhizal fungi(AMF)play a vital role in plant productivity and ecosystem ***,their responses to abiotic factors(i.e.,climate,physiography,and soil properties)are unknown,especially across climatic gradients and slope aspects in arid and semi-arid *** this study,using 60 composite soil samples,direct and indirect effects of climate factors and slope aspects on AMF diversity,composition and spore density were *** findings indicate that climate has a more direct influ-ence on soil properties(P<0.001)in comparison to slope aspect(P=0.449).In contrast,climate significantly affected AMF diversity and composition,with the highest diversity in dryer *** pH had the highest correlation with different facets of AMF *** equation modeling(SEM)indicated that only a small part of the variation in AMF diversity and spore density could be explained by climate characteristics,slope aspect and soil *** on SEM results,climate was the most important determinant of AMF diversity and spore density;slope aspect had a less critical *** outputs suggest that variations in AMF diversity are derived by the direct effects of climate and the indirect effect of soil chemical *** addition,with increasing dryness,sporulation and AMF diversity increased.

关键词： AMF diversity and composition Physiographic factors Semi-arid ecosystems Zagros forest

Theaflavin-3，3′-digallate alleviates glucolipotoxicity-induced insulin secretion dysfunction in pancreatic β-TC-6 cells and zebrafish via anti-ferroptotic mechanisms by activating SERCA2

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Food science and Human Wellness 2024年

作者： Kaixi Wang Huanqing Zhu Jiaxin Kang Shuna Chen Puming He Youying Tu Bo Li department of Tea science， College of agriculture and Biotechnology， Zhejiang University

Theaflavins from black tea effectively improve insulin secretion in obesity and diabetes， but the molecular mechanisms are unclear. Here， the palmitic acid (PA)-induced pancreatic β-TC-6 cells and high fat-/high glucose-induced zebrafish were used. The effects of theaflavin-3，3′-digallate (TF3) on glucolipotoxicity-induced insulin secretion dysfunction， ferroptosis and endoplasmic reticulum (ER) stress were investigated by a variety of molecular biological approaches， inductively coupled plasma-mass spectrometry， transmission electron microscopy and widely targeted metabolomics analysis. TF3 was found to potently inhibit glucolipotoxicity-induced insulin secretion dysfunction and ferroptosis in β-TC-6 cells and zebrafish， with increasing glutathione peroxidase 4 (GPX4) expression， suppressing lipid peroxidation and iron accumulation and protecting mitochondria. Additionally， TF3 attenuated ER stress by regulating three unfolded protein response (UPR) pathways in β-TC-6 cells， and significantly modulated linoleic acid metabolism and L-kynurenine (Kyn) signalling in zebrafish. The expression of sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (SERCA2) was obviously enhanced by TF3. Thapsigargin (Tg)， a SERCA2 inhibitor， remarkably reversed the effects of TF3 on insulin production， ferroptosis， ER stress and the Kyn signalling. Together， this work revealed for the first time the critical role of SERCA2 in ferroptosis regulation， and demonstrated TF3 targeted SERCA2 to inhibit ER stress and ferroptosis， thereby protecting β-cell secretory function from glucolipotoxicity.

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Plant-available water capacity of soils at a regional scale:Analysis of fixed and dynamic field capacities

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Pedosphere 2024年第3期34卷 590-605页

作者： Vinod PHOGAT Paul RPETRIE Casandra COLLINS Marcos BONADA South Australian Research and Development Institute GPO Box 397Adelaide SA 5001(Australia) School of agriculture Food and WineWaite Research InstituteUniversity of AdelaideWaite CampusPMB1Glen Osmond SA 5064(Australia) College of science and Engineering Flinders UniversityBedford Park SA 5042(Australia) The University of New South Wales Sydney NSW 2052(Australia) ARC Training Centre for Innovative wine Production University of AdelaidePMB1Glen Osmond SA 5064(Australia)

Estimation of the plant-available water capacity(PAWC)of soils at a regional scale helps in adopting better land use planning,developing suitable irrigation schedules for crops,and optimizing the use of scarce water *** the current study,72 soil profiles were sampled from the Barossa region of South Australia to estimate pedo-transfer functions deduced from easily estimated soil *** functions were then used to estimate the fixed(10 and 33 kPa)and dynamic pressure head(h_(fc))water contents at field capacity(FC)for minimum drainage flux(0.01 and 0.001 cm d^(-1)),which serves as the upper boundary for plant-available water in *** estimated residual water content was corrected for subsoil constraints,especially the exchangeable sodium percentage(ESP).The results showed that the mean values of h_(fc)in sand-dominated light and medium textured soils(i.e.,sand,loamy sand,sandy loam,and loam)varied in a narrow range(15.8-18.2 kPa),whereas those in the clay-dominated heavy textured soils(i.e.,clay loam)showed a wide range(11.3-49.3 kPa).There were large differences in PAWC for dynamic FC(PAWC_(fc))and fixed FC at 10 kPa(PAWC10),33 kPa(PAWC33),and a mix of 10and 33 kPa(PAWC_(10,33))pressure heads depending on soil ***,the difference between PAWC at 10 kPa and h_(fc)(ΔPAWC_(10))was positive,whereas that between 33 kPa and h_(fc)(ΔPAWC_(33))was negative across all ***,the estimation of PAWC assuming a fixed FC at 10 and 33 kPa pressures(i.e.,PAWC_(10,33))for sandy,clay,and silty soils reduced the difference between fixed and dynamic pressure PAWCs to<10% across the *** estimation of PAWC was improved by incorporating the impact of subsoil constraints,such as high ESP,which was more pronounced for clay and silty *** findings demonstrate the inherent inconsistencies between static pressure and flux-based dynamic FC estimations in *** heterogeneity,intra-texture variability,subsoil constraints,and swell

关键词： crop lower limit drained upper limit pedo-transfer function soil hydraulic parameter soil texture

Identif ication of a hypersensitive response core peptide of HrpZ and its role in increasing grape downy mildew resistance

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Horticultural Plant Journal 2024年第5期10卷 1112-1120页

作者： Zongbao Fan Xueqiang Guan Zhichang Zhang Yushuai Sun Fei Wang Huiru Chi Yuxin Yao State Key Laboratory of Crop Biology Collaborative Innovation Center of Fruit&Vegetable Quality and Efficient ProductionCollege of Horticulture Science and EngineeringShandong Agricultural UniversityTai'anShandong 271018China Shandong Academy of Grape/Shandong Technology Innovation Center of wine Grape and wine JinanShandong 250100China Zhichang Agricultural science and Technology Development Co. Ltd.RizhaoShandong 276511China

Harpins play a key role in inducing disease resistance in crops,and identifying their core functional regions and establishing a system for their efficient expression would be very *** this study,large amounts of soluble fusion proteins of harpin HrpZ and its subpeptides were obtained via the optimized induction conditions(28C with 0.5 mmol$L1 IPTG for 6 h)in Escherichia coli BL21(DE3).Hypersensitive response(HR)assays demonstrated that the C-terminal 66 aa of HrpZ(HrpZ_C_2_2)elicited a strong HR in tobacco(Nicotiana benthamiana)and grape(Flame Seedless)***,treatment with HrpZ,and particularly HrpZ_C_2_2,significantly reduced the disease incidence and severity index of field vine leaves and those inoculated with downy *** determination of the physiological parameters indicated that HrpZ,and especially HrpZ_C_2_2,improved the photosynthesis-and chlorophyll fluorescence-related parameters,enhanced the activity of defense-related enzymes,including SOD,POD,CAT and PAL,and increased the H_(2)O_(2)***,we efficiently expressed a core peptide of HrpZ and elucidated its strong ability to elicit a HR and resistance to downy *** research provides insight into understanding the structure and function of HrpZ and will advance the application of HrpZ_C_2_2 to increase the resistance of grapevine to downy mildew.

关键词： Grapevine Harpin HrpZ Prokaryotic expression Downy mildew Hypersensitive response

Comparative metabolomics reveal histamine biodegradation mechanism by salt stressed Bacillus subtilis JZXJ-7

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Food science and Human Wellness 2024年

作者： Rundong Wang Yijia Deng Yuhao Zhang Xuepeng Li Ravi Gooneratne Jianrong Li College of Food science and Engineering， Bohai University Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing department of wine， Food and Molecular Biosciences， Faculty of agriculture and Life sciences， Lincoln University Lincoln 7647， New Zealand College of Food science， Southwest University College of Food science and Engineering， Lingnan Normal University

Bacillus subtilis (B. subtilis) JZXJ-7 isolated from shrimp paste can significantly degrade histamine under salt stress but the mechanism is unclear. This study aims to evaluate the effect of 170 and 340 mmol/L NaCl on B. subtilis JZXJ-7 growth， histamine degradation， antioxidant enzymes [catalase (CAT)， superoxide dismutase (SOD) and glutathione S-transferase (GST)] activities and Na+， K+-ATPase activity. Furthermore， comparative metabolomics was used to investigate histamine biodegradation mechanism by *** JZXJ-7 subjected to salt stress. Both 170 and 340 mmol/L NaCl promoted B. subtilis JZXJ-7 growth in late stages of reproduction (32 - 48 h)， increased histamine degradation rate by 64.85% and 79.87% (P < 0.05)， Na+， K+-ATPase activity to 6.28 U/mg (P < 0.05) and 11.63 U/mg (P < 0.01) respectively. NaCl treatment significantly increased the activities of CAT， GST and SOD (P < 0.05)， amino acids and its metabolites (33.39%)， benzene and substituted derivatives (12.05%)， heterocyclic compounds (10.62%)， organic acids and derivatives (9.75%)， aldehydes， ketones， esters (5.59%) and nucleotides and its metabolites (4.58%). Metabolite set enrichment analysis revealed NaCl induced differential metabolic pathways of D-glutamine， D-glutamate， L-arginine， L-proline， histidine and glycerophospholipids， L-lysine degradation， and aminoacyl-tRNA biosynthesis. Exposure to 340 mmol/L NaCl up-regulated carbohydrate， glutathione and glycerophospholipid metabolism. The new insights into the mechanism of salt stress to promote B. subtilis JZJX-7 growth， energy metabolic pathways and to degrade histamine provide the theoretical basis for application of B. subtilis JZXJ-7 in food fermentation industry.

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Multi‑omics integration identifies regulatory factors underlying bovine subclinical mastitis

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Journal of Animal science and Biotechnology 2024年第3期15卷 987-1007页

作者： Mengqi Wang Naisu Yang Mario Laterriere David Gagne Faith Omonijo Eveline M.Ibeagha‑Awemu agriculture and Agri‑Food Canada Sherbrooke Research and Development CentreSherbrookeQCCanada department of Animal science Universite LavalQuebec CityQCCanada Quebec Research and Development Centre Agriculture and Agri-Food CanadaQuebec CityQCCanada

Background Mastitis caused by multiple factors remains one of the most common and costly disease of the dairy ***-omics approaches enable the comprehensive investigation of the complex interactions between mul-tiple layers of information to provide a more holistic view of disease ***,this study investigated the genomic and epigenomic signatures and the possible regulatory mechanisms underlying subclinical mastitis by integrating RNA sequencing data(mRNA and lncRNA),small RNA sequencing data(miRNA)and DNA methylation sequencing data of milk somatic cells from 10 healthy cows and 20 cows with naturally occurring subclinical mastitis caused by Staphylococcus aureus or Staphylococcus *** Functional investigation of the data sets through gene set analysis uncovered 3458 biological process GO terms and 170 KEGG pathways with altered activities during subclinical mastitis,provided further insights into subclin-ical mastitis and revealed the involvement of multi-omics signatures in the altered immune responses and impaired mammary gland productivity during subclinical *** abundant genomic and epigenomic signatures with sig-nificant alterations related to subclinical mastitis were observed,including 30,846,2552,1276 and 57 differential methylation haplotype blocks(dMHBs),differentially expressed genes(DEGs),lncRNAs(DELs)and miRNAs(DEMs),***,5 factors presenting the principal variation of differential multi-omics signatures were *** important roles of Factor 1(DEG,DEM and DEL)and Factor 2(dMHB and DEM),in the regulation of immune defense and impaired mammary gland functions during subclinical mastitis were *** of the omics within Factors 1 and 2 explained about 20%of the source of variation in subclinical ***,networks of impor-tant functional gene sets with the involvement of multi-omics signatures were demonstrated,which contributed to a comprehensive view of the possible regu

关键词： Candidate discriminant multi-omics signature Gene Long non-coding RNA Methylation haplotype block MicroRNA Multi-omics integration Natural killer cell mediated cytotoxicity pathway Staphylococcus aureus infection pathway

An updated inventory of marine opisthobranch(Mollusca,Gastropoda)from the territorial waters of the Republic of Mauritius

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Journal of Oceanology and Limnology 2024年第1期42卷 226-243页

作者： Lisa AH SHEE TEE Chandani APPADOO Daneshwar PUCHOOA Vishwakalyan BHOYROO department of Agricultural&Food science Faculty of AgricultureUniversity of MauritiusRéduit 80837Mauritius department of Biosciences&Ocean Studies Pole of Research Excellence Sustainable Marine BiodiversityFaculty of ScienceUniversity of MauritiusRéduit 80837Mauritius

Island ecosystems support diverse aquatic invertebrate communities comprising endemic *** of existing species is important for *** this study,a checklist of marine opisthobranch from the Republic of Mauritius is presented.A combination of benthic surveys(50 m×5 m in triplicates),rover diving techniques and photo documentation were used over two years(2018–2020)within 35 sheltered and unsheltered *** and molecular analysis were used for *** composition within sheltered and unsheltered areas in Mauritius was estimated using the Bray-Curtis *** checklist featured 117 species belonging to 61 genera and 28families,of which 13 are new *** findings increased the knowledge of opisthobranch diversity from the Mauritius by 15.4%.Among the listed species,the distribution range of Cyerce nigra,Actinocyclus papillatus,and Phyllidia picta extended from the Western Pacific to the South Western Indian *** analysis of the undescribed Gymnodoris *** it resembled Gymnodoris *** Hawaii and were different by a genetic distance value of 10.6%.The species richness and evenness were higher within the sheltered regions of Mauritius which harboured the food resource of *** areas as compared to unsheltered regions were heavily populated,suggesting the probable influence of wave actions on opisthobranch diversity and *** order Nudibranchia was reported as most speciose,with 86 *** Sacoglossa and Nudibranchia were observed only on macroalgae and sponges *** abundance was also recorded on shipwrecks which are the most common form of artificial *** the inclusion of observations from previous studies,201species belonging to 94 genera and 36 families are now known from the Mauritius.

关键词： marine opisthobranch inventory new record occurrence Republic of Mauritius Indian Ocean

Enhanced Topic-Aware Summarization Using Statistical Graph Neural Networks

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Computers, Materials & Continua 2024年第8期80卷 3221-3242页

作者： Ayesha Khaliq Salman Afsar Awan Fahad Ahmad Muhammad Azam Zia Muhammad Zafar Iqbal department of Computer science University of Agriculture FaisalabadFaisalabadPunjab37300Pakistan School of Computing Faculty of TechnologyUniversity of PortsmouthSouthseaPortsmouthPO12UPUK department of Mathematics and Statistics University of Agriculture FaisalabadFaisalabadPunjab37300Pakistan

The rapid expansion of online content and big data has precipitated an urgent need for efficient summarization techniques to swiftly comprehend vast textual documents without compromising their original *** approaches in Extractive Text Summarization(ETS)leverage the modeling of inter-sentence relationships,a task of paramount importance in producing coherent *** study introduces an innovative model that integrates Graph Attention Networks(GATs)with Transformer-based Bidirectional Encoder Representa-tions from Transformers(BERT)and Latent Dirichlet Allocation(LDA),further enhanced by Term Frequency-Inverse Document Frequency(TF-IDF)values,to improve sentence selection by capturing comprehensive topical *** approach constructs a graph with nodes representing sentences,words,and topics,thereby elevating the interconnectivity and enabling a more refined understanding of text *** model is stretched to Multi-Document Summarization(MDS)from Single-Document Summarization,offering significant improvements over existing models such as THGS-GMM and Topic-GraphSum,as demonstrated by empirical evaluations on benchmark news datasets like Cable News Network(CNN)/Daily Mail(DM)and *** results consistently demonstrate superior performance,showcasing the model’s robustness in handling complex summarization tasks across single and multi-document *** research not only advances the integration of BERT and LDA within a GATs but also emphasizes our model’s capacity to effectively manage global information and adapt to diverse summarization challenges.

关键词： Summarization graph attention network bidirectional encoder representations from transformers Latent Dirichlet Allocation term frequency-inverse document frequency

Gut microbiota intervention attenuates thermogenesis in broilers exposed to high temperature through modulation of the hypothalamic 5‑HT pathway

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Journal of Animal science and Biotechnology 2024年第2期15卷 679-698页

作者： Sheng Li Xiaoqing Li Kai Wang Yansen Li Kentaro Nagaoka Chunmei Li Research Centre for Livestock Environmental Control and Smart Production College of Animal Science and TechnologyNanjing Agricultural UniversityNanjing 210095China Laboratory of Veterinary Physiology Department of Veterinary MedicineFaculty of AgricultureTokyo University of Agriculture and TechnologyTokyo 183‑8509Japan

Background Broilers have a robust metabolism and high body temperature,which make them less tolerant to hightemperature(HT)environments and more susceptible to challenges from elevated *** microbes,functioning as symbionts within the host,possess the capacity to significantly regulate the physiological functions and environmental adaptability of the *** study aims to investigate the effects of gut microbial intervention on the body temperature and thermogenesis of broilers at different ambient temperatures,as well as the underlying mechanism involving the"gut-brain"*** Broilers were subjected to gut microbiota interference with or without antibiotics(control or ABX)starting at 1 day of *** 21 day of age,they were divided into 4 groups and exposed to different environments for 7 d:The control and ABX groups at room temperature(RT,24±1℃,60%relative humidity(RH),24 h/d)and the control-HT and ABX-HT groups at high temperature(HT,32±1℃,60%RH,24 h/d).Results The results demonstrated that the antibiotic-induced gut microbiota intervention increased body weight and improved feed conversion in broiler chickens(P<0.05).Under HT conditions,the microbiota intervention reduced the rectal temperature of broiler chickens(P<0.05),inhibited the expression of avUCP and thermogenesisrelated genes in breast muscle and liver(P<0.05),and thus decreased thermogenesis ***,the gut microbiota intervention blunted the hypothalamic‒pituitary‒adrenal axis and hypothalamic–pituitary–thyroid axis activation induced by HT *** analyzing the cecal microbiota composition of control and ABX chickens maintained under HT conditions,we found that Alistipes was enriched in control *** contrast,antibioticinduced gut microbiota intervention resulted in a decrease in the relative abundance of Alistipes(P<0.05).Moreover,this difference was accompanied by increased hypothalamic 5-hydroxytryptamine(5-HT)content and TPH2 expression(P<0.05).Conclus

关键词： 5-HT Broiler chickens Gut microbiota Thermogenesis Thermoregulation