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Phenolic Profile and Volatiles of in vitro Propagated Lavandula angustifolia ***

Phyton-International Journal of Experimental Botany 2024年第3期93卷 427-444页

作者： Mir Abdullatif Yahya Fatma Tunali Dilek Killi Atalay Sökmen Biotechnology department Konya Food and Agriculture UniversityKonya42080Türkiye Plant Production and Technologies department Konya Food and Agriculture UniversityKonya42080Türkiye

An effective in vitro propagation protocol was designed for Lavandula angustifolia Miller,a medicinal aromatic plant that is a prominent source of volatile organic compounds(VOCs).Murashige and Skoog media were supplemented with various concentrations of Plant Growth Regulators(PGRs),and the growth parameters of the nodal segments were *** explants formed callus when they were supplemented with 2 mg/L of 6-Benzylaminopurine(BAP).The superior hormonal concentration of Murashige and Skoog(MS)media for the proliferation of shoots from callus cultures(39.33%)was 5 mg/L of 2-Isopentenyl adenine(2iP),and the favorable media for the growth of *** callus cultures was 1 and 2 mg/L of BAP,with a 98%for-mation rate in each *** callus cultures and in vitro propagated *** seedlings obtained from var-ious PGR concentrations of MS media were exposed to qualitative and quantitative analysis in terms of phenolic proﬁles,ﬂavonoids,High-performance liquid chromatography(HPLC)analysis of phenolic acids,and headspace-SPME analysis for *** analysis revealed that micropropagated seedlings grown in media containing 1 mg/L of 6-Furfurylaminopurine(KIN)accumulated the highest yield(11.95±0.01 mg GAE/g)of phenolic *** contrast,the lowest concentration(2.17±0.04 mg GAE/g)was detected in 0.5 mg/L of BAP+0.5 mg/L of Naphthaleneacetic acid(NAA)*** plantlets grown in 0.5 mg/L of BAP+0.5 mg/L of NAA media showed the highestﬂavonoid yield(31.67±0.06μg/g QE/g).In contrast,callus samples exhibited the lowest yield(11.59±0.02μg/g QE/g)ofﬂavonoids in MS media supplemented with a concentration of 0.5 mg/L of *** analysis revealed the variability of phenolic acid contents within the callus cultures as well as plantlets,with gallic acid,4-OH benzoic acid,chlorogenic acid,vanillic acid,caffeic acid,cinnamic acid,and rosmarinic acid being the prominent *** presence of twenty-two chemicals was revealed by headspace-SPME ***

关键词： Lavender indirect organogenesis micropropagation This

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A plant growth chamber system equipped with aerosol generators for studying aerosol-vegetation interactions

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Particuology 2024年第2期85卷 122-132页

作者： Masao Gen Seiji Ikawa Masahiro Yamaguchi Fong Zyin Lim Takeshi Izuta I.Wuled Lenggoro Graduate School of Bio-Applications and Systems Engineering Tokyo University of Agriculture and TechnologyKoganeiTokyo184-8588Japan Institute of Multidisciplinary Research for Advanced Materials Tohoku UniversitySendai980-8577Japan Faculty of agriculture Tokyo University of Agriculture and TechnologyFuchuTokyo183-8509Japan Graduate School of Fisheries and Environmental Sciences Nagasaki UniversityNagasaki852-8521Japan Institute of agriculture Tokyo University of Agriculture and TechnologyFuchuTokyo183-8509 Japan department of Applied Physics and Chemical Engineering Tokyo University of Agriculture and TechnologyKoganeiTokyo184-8588Japan Institute of Global Innovation Research Tokyo University of Agriculture and TechnologyFuchuTokyo183-8509Japan

Understanding aerosol-vegetation interactions is vital in ***,the interactions remain elusive partly due to the lack of suitable plant growth chamber ***,deposition of submicron particles on leaf surfaces is challenging due to its low deposition velocities compared to larger *** this work,we present a plant-growth chamber that was used to study the effect of sub-micron black carbon(BC)particles on the growth and photosynthesis of *** chamber system simultaneously enables the growth of multiple plants in pots and the deposition of submicron particles onto *** spraying methods assisted by ultrasonic and electrostatic forces were employed as aerosol generators to realize the particle *** flow regime inside the chamber was numeri-cally calculated to predict the transportation of aerosol particles,suggesting the optimal operating conditions of the *** gas-phase particle size distribution measurements showed that gener-ated BC particles were suspended in submicron diameter *** aerosol generators were examined in the chamber using three conductor and insulator substrates as a model of plant *** observations and spectroscopic analysis ascertained that submicron BC particles generated from our generators were deposited on all substrate *** the developed chamber system,systematic studies can be performed to advance the fundamental understanding of aerosol-vegetation interactions.

关键词： Aerosol Exposure chamber Spray drying Black carbon

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Fuzzy Reliability and Availability of System under a Calendar-based Inspection Involving Multiple Failures and Its Application to Wind Turbine System

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Journal of Systems Science and Systems Engineering 2024年第2期33卷 187-206页

作者： Mintu Kumar Himani Pant S.B.Singh department of Mathematics Statistics and Computer Science G.B.Pant University of AgricultureTechnologyPantnagarUttarakhandIndia

Uncertainty is an important factor that needs to be considered while analyzing the performance of any engineering *** order to quantify uncertainty,fuzzy set theory is frequently used by most of researchers,including energy system *** to the classical reliability theory,component lifetimes have crisp parameters,but due to uncertainty and inaccuracy in data,it is sometimes very difficult to determine the exact values of these parameters in real-world *** overcome this difficulty in the current research,failure and repair rates were taken as triangular fuzzy numbers to determine the fuzzy availability of a system undergoing calendar-based periodic inspection subject to multiple failure modes(FMs).It was assumed that each component in the system had an exponential failure rate and repair rate with fuzzy *** FMs were explicitly taken into account when a functional state of the system was *** FM had a random failure *** the occurrence of any failure,a random time was selected for the relevant corrective repair *** proposed research was studied for one of the major sources of green energy,namely a wind turbine system wherein all the derived propositions have been implemented on it.

关键词： Fuzzy reliability fuzzy availability wind turbine system multiple failure modes triangular fuzzy number

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Methionine sources and genotype affect embryonic intestinal development, antioxidants, tight junctions, and growth-related gene expression in chickens

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Animal Nutrition 2024年第1期16卷 218-230页

作者： James K.Lugata Sawadi F.Ndunguru Gebrehaweria K.Reda Xénia E.Ozsváth Eszter Angyal Levente Czeglédi Gabriella Gulyás Renáta Knop János Oláh Zoltán Mészár Rita Varga Brigitta Csernus Csaba Szabó department of Animal Nutrition and Physiology Faculty of Agriculture and Food Sciences and Environmental Management University of Debrecen Faculty of agriculture and Food Sciences and Environmental Management Doctoral School of Animal Science University of Debrecen department of Animal Husbandry Faculty of Agriculture and Food Sciences and Environmental Management University of Debrecen department of Evolutionary Zoology and Human Biology Faculty of Science and Technology University of Debrecen Institutes for Agricultural Research and Educational Farm University of Debrecen department of Anatomy Histology and Embryology Faculty of Medicine University of Debrecen

Methionine(Met) is an essential and first limiting amino acid in the poultry diet that plays a significant role in chicken embryonic development and growth. The present study examined the effect of in ovo injection of DL-Met and L-Met sources and genotypes on chicken embryonic-intestinal development and health. Fertilized eggs of the two genotypes, TETRA-SL layer hybrid(TSL)—commercial layer hybrid and Hungarian Partridge colored hen breed(HPC)—a native genotype, were randomly distributed into four treatments for each genotype. The treatment groups include the following: 1) control non-injected eggs(NoIn); 2) saline-injected(SaIn); 3) DL-Met injected(DLM); and 4) L-Met injected(LM). The in ovo injection was carried out on 17.5 d of embryonic development; after hatching, eight chicks per group were sacrificed, and the jejunum was extracted for analysis. The results showed that both DLM and LM groups had enhanced intestinal development as evidenced by increased villus width, villus height, and villus area(P < 0.05) compared to the control. The DLM group had significantly reduced crypt depth, glutathione(GSH) content, glutathione S-transferase 3 alpha(GST3), occludin(OCLN) gene expression and increased villus height to crypt depth ratio in the TSL genotype than the LM group(P < 0.05). The HPC genotype has overexpressed insulin-like growth factor 1(IGF1) gene, tricellulin(MD2), occludin(OCLN),superoxide dismutase 1(SOD1), and GST3 genes than the TSL genotype(P < 0.05). In conclusion, these findings showed that in ovo injection of Met enhanced intestinal development, and function, with genotypes responding differently under normal conditions. Genotypes also influenced the expression of intestinal antioxidants, tight junction, and growth-related genes.

关键词： DL-Methionine L-Methionine In ovo injection Intestinal development Poultry

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Ammonia and greenhouse gas distribution in a dairy barn during warm periods

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Frontiers of Agricultural Science and Engineering 2024年第3期11卷 428-441页

作者： Provvidenza Rita D'URSO Claudia ARCIDIACONO Giovanni CASCONE department of agriculture Food and Environment University of Catania

This research aimed to quantify concentrations of ammonia（NH3）, carbon dioxide（CO2） and methane（CH4）, estimate emissions, and analyze the factors influencing them during warm periods in an open dairy barn equipped with two cooling systems in a Mediterranean climate zone. Gas distribution within the barn was observed to vary both vertically and horizontally, with the highest gas concentrations observed in the central area of the barn. NH3, CH4and CO2ranged in 1.7–7.4, 7–18, 560–724 μg·g–1, respectively. Natural ventilation through openings and the operation of cooling systems induced changes in indoor microclimate conditions, influencing cow behavior and,consequently, gas production. Gas concentrations were the highest at air velocities below 0.5 m·s–1. The highest concentration of NH3was observed when the temperature-humidity index（THI） was > 72 and ≤ 78; and CO2and CH4concentrations were the highest with THI ≥ 72 and decreased with THI ≤72. NH3concentrations when barn management included three daily milkings were higher than those measured when barn management was based on two daily milkings, and lower for CH4and CO2. NH3and CH4emissions were the highest during barn cleaning, while the lowest NH3emissions occurred during activity of the cows（i.e., feeding, walking）.

关键词： Ammonia greenhouse gas environmental monitoring cows’ behavior barn management housing system climatic parameters

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Rice ONAC016 promotes leaf senescence through abscisic acid signaling pathway involving OsNAP

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The Crop Journal 2024年第3期12卷 709-720页

作者： Eunji Gi Sung-Hwan Cho Suk-Hwan Kim Kiyoon Kang Nam-Chon Paek department of agriculture Forestry and BioresourcesPlant Genomics and Breeding InstituteResearch Institute of Agriculture and Life SciencesSeoul National UniversitySeoul 08826Republic of Korea Division of Life Sciences Incheon National UniversityIncheon 22012Republic of Korea

Senescence-induced NAC(senNAC)TFs play a crucial role in senescence during the final stage of leaf *** this study,we identified a rice senNAC,ONAC016,which functions as a positive regulator of leaf *** expression of ONAC016 increased rapidly in rice leaves during the progression of dark-induced and natural *** onac016-1 knockout mutant showed a delayed leaf yellowing phenotype,whereas the overexpression of ONAC016 accelerated leaf ***,ONAC016 expression was upregulated by abscisic acid(ABA),and thus detached leaves of the onac016-1 mutant remained green much longer under ABA *** RT-PCR analysis showed that ONAC016 upregulates the genes associated with chlorophyll degradation,senescence,and ABA *** one-hybrid and dual-luciferase assays revealed that ONAC016 binds directly to the promoter regions of OsNAP,a key gene involved in chlorophyll degradation and ABA-induced *** together,these results suggest that ONAC016 plays an important role in promoting leaf senescence through the ABA signaling pathway involving OsNAP.

关键词： Rice ONAC016 OsNAP Leaf senescence Abscisic acid signaling

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Fermented calcium butyrate supplementation in post-peak laying hens improved ovarian function and tibia quality through the“gut-bone” axis

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Animal Nutrition 2024年第1期16卷 350-362页

作者： Huaiyong Zhang Yongshuai Wang Yilu Wang Bin Wei Leilei Wang Minh Tu Nguyen Xiangyun Lv Yanqun Huang Wen Chen College of Animal Science and Technology Key Laboratory of Animal Biochemistry and NutritionMinistry of AgricultureHenan Agricultural University Laboratory for Animal Nutrition and Animal Product Quality Department of Animal Sciences and Aquatic EcologyGhent University department of agriculture and Forestry Hue University Charoen Pokphand Group Co. Ltd.

The compromised egg quality and leg abnormality during the end of the laying cycle(after 40 weeks)have been leading to poor animal welfare and substantial economic losses. Therefore, the effects of fermented calcium(Ca) butyrate, produced by fermentation by Clostridium butyricum, on production,eggshell quality, and tibial property of hens were explored. A total of 192 Hy-line brown laying hens at50-week-old were assigned to a basal diet or the basal diet with 300 mg/kg of the fermented Ca butyrate from 50 to 58 weeks of age. Each treatment had 6 replicates with 16 hens each. The diet supplemented with 300 mg/kg fermented Ca butyrate notably increased egg weight, ovarian follicle number, and eggshell strength(P = 0.072) as compared to the basal diet, which were associated with cytokine secretion, toll-like receptor signaling pathways, and intestinal immunity based on the RNA-seq data from the granulosa. Dietary Ca butyrate inclusion decreased the expression of ileal tumor necrosis factor-alpha and serum pro-inflammatory cytokine concentration, as well as increased the content of serum immunoglobulin A when compared to the basal diet(both P 0.05). In addition, the diet with fermented Ca butyrate reduced the number of osteoclasts in the proximal tibia and the level of C-terminal cross-linked telopeptide of type I collagen, a bone resorption marker(P < 0.05), whereas it tended to increase the concentration of the procollagen type I N-terminal propeptide that reflects bone formation marker in serum. Moreover, the layers fed fermented Ca butyrate diets possessed higher(P < 0.05) bone area and trabecular number of the proximal tibia, yield load, and ultimate load than those that consumed basal diets. Collectively, dietary fermented Ca butyrate supplementa

关键词： Fermented Ca butyrate Ovarian property Tibia metabolism Layer

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Multi‑omics integration identifies regulatory factors underlying bovine subclinical mastitis

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Journal of Animal Science and Biotechnology 2024年第3期15卷 987-1007页

作者： Mengqi Wang Naisu Yang Mario Laterriere David Gagne Faith Omonijo Eveline M.Ibeagha‑Awemu agriculture and Agri‑Food Canada Sherbrooke Research and Development CentreSherbrookeQCCanada department of Animal Science Universite LavalQuebec CityQCCanada Quebec Research and Development Centre Agriculture and Agri-Food CanadaQuebec CityQCCanada

Background Mastitis caused by multiple factors remains one of the most common and costly disease of the dairy ***-omics approaches enable the comprehensive investigation of the complex interactions between mul-tiple layers of information to provide a more holistic view of disease ***,this study investigated the genomic and epigenomic signatures and the possible regulatory mechanisms underlying subclinical mastitis by integrating RNA sequencing data(mRNA and lncRNA),small RNA sequencing data(miRNA)and DNA methylation sequencing data of milk somatic cells from 10 healthy cows and 20 cows with naturally occurring subclinical mastitis caused by Staphylococcus aureus or Staphylococcus *** Functional investigation of the data sets through gene set analysis uncovered 3458 biological process GO terms and 170 KEGG pathways with altered activities during subclinical mastitis,provided further insights into subclin-ical mastitis and revealed the involvement of multi-omics signatures in the altered immune responses and impaired mammary gland productivity during subclinical *** abundant genomic and epigenomic signatures with sig-nificant alterations related to subclinical mastitis were observed,including 30,846,2552,1276 and 57 differential methylation haplotype blocks(dMHBs),differentially expressed genes(DEGs),lncRNAs(DELs)and miRNAs(DEMs),***,5 factors presenting the principal variation of differential multi-omics signatures were *** important roles of Factor 1(DEG,DEM and DEL)and Factor 2(dMHB and DEM),in the regulation of immune defense and impaired mammary gland functions during subclinical mastitis were *** of the omics within Factors 1 and 2 explained about 20%of the source of variation in subclinical ***,networks of impor-tant functional gene sets with the involvement of multi-omics signatures were demonstrated,which contributed to a comprehensive view of the possible regu

关键词： Candidate discriminant multi-omics signature Gene Long non-coding RNA Methylation haplotype block MicroRNA Multi-omics integration Natural killer cell mediated cytotoxicity pathway Staphylococcus aureus infection pathway

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Impact of numerous larval diets on the biology of pink bollworm Pectinophora gossypiella(Saunders)(Lepidoptera:Gelechiidae)under laboratory conditions

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Journal of Cotton Research 2024年第1期7卷 69-78页

作者： AKHTAR Shamim ARIF Muhammad Jalal GOGI Muhammad Dildar HAQ Imran-ul department of Entomology University of Agriculture FaisalabadPunjabPakistan Ayub Agricultural Research Institute(Entomological Research Institute) FaisalabadPunjabPakistan department of Plant Pathology University of AgricultureFaisalabadPunjabPakistan

Background Pink bollworm,Pectinophora gossypiella(Saunders)(Lepidoptera:Gelechiidae)has become a poten-tial pest of cotton by causing substantial yield losses around the world including *** in view the facts like limited research investigations,unavailability,and high cost of artificial diet’s constituents and their premixes,the present research investigations on the dietary aspect of *** were *** larvae of *** were reared on different diets that were prepared using indigenous *** standard/laboratory diet com-prised of wheat germ meal 34.5 g,casein 30.0 g,agar–agar 20.0 g,sucrose 10.0 g,brewer’s yeast 5.0 g,α-cellulose 1.0 g,potassium-sorbate1.5 g,niplagin 0.5 g,decavitamin 0.01 g,choline-chloride 0.06 g,maize-oil 3.30 g,honey 2.0 g,and water 730.0 *** to cotton bolls and wheat germ meal were okra seed sprouts,okra fruit,cottonseed meal,and okra seed meals,which were included in the study to introduce an efficient and economic mass-rearing *** The larval development completed in 19.68d±0.05 d with a weight of 20.18mg±0.20 mg at the fourth instar fed on the cottonseed meal-based diet instead of wheat germ meal based *** the same diet,84.00%±4.00%,17.24 mg±0.03 mg,and 7.76d±0.06 d were recorded as pupae formation,pupal weight,and pupal duration,*** emergence,76.00%±1.00%was recorded from pupae collected from larvae raised on cottonseed meal-based *** male and female moths lived for 40.25d±0.10 d,and 44.34d±0.11 d,*** deposited 21.28±0.04 eggs per day with the viability of 65.78%±0.14%.The larval mortal-ity at the fourth instar was 37.20%±1.36%and malformed pupation of 12.00%±1.41%was *** of wheat germ meal with that of local meals(cottonseed and okra seed)in the standard laboratory diet has saved 463.80 to 467.10 PKR with 1.62 to 1.63 cost economic returns,*** This research is of novel nature as it provides a concis

关键词： Artificial diets Cottonseed meal Laboratory rearing Pectinophora gossypiella Wheat germ meal

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Evaluation of the nutritional value of Artemia nauplii for European seabass(Dicentrarchus labrax L.)larvae

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Aquaculture and Fisheries 2024年第1期9卷 78-84页

作者： Alaa A.El-Dahhar Rashwan S.Rashwan Samy Y.EL-Zaeem Shaimaa A.Shahin Mona M.Mourad Mohammed F.El Basuini Animal and Fish Production department Faculty of Agriculture-Saba BashaAlexandria UniversityAlexandria21526Egypt National Institute of Oceanography and Fisheries(NIOF) Cairo11562Egypt department of Animal Production Faculty of AgricultureTanta UniversityTanta31527Egypt Faculty of Desert agriculture King Salman International UniversitySouth Sinai46618Egypt

Adapted European seabass(Dicentrarchus labrax L.)larvae at 25 days post-hatching(dph)with a primary weight and length of 23 mg and 9.92 mm,start the weaning up to 46 dph using the weaning micro-diet(54%crude protein from fish meal,powder milk,and poultry egg)concurrently with Artemia enriched with fortification emulsions(0.6 g/L)of DHA selco®as a control group(DHAS)or Fish oil+20%Vitamin C(FOVC),or Fish oil+20%Vitamin E(FOVE),or Fish oil+10%Vit.C+10%Vit.E(FOCE),or Fish oil only(FO).At 46 dph,groups of larvae fed enriched Artemia with DHAS and FOVE exhibited the highest final body weight,weight gain,average daily gain,specific growth rate,feed intake,protein efficiency ratio,and survival%as well as the lowest feed conversion ***,larvae in FOVC displayed the lowest final body weight,total length,weight gain,average daily gain,specific growth rate,feed intake,protein efficiency ratio,and survival%as well as the highest feed conversion *** histological assessment exhibited no pathological alteration as well as an improvement in the structure with DHAS,FOVE,FOVC,and FOCE co-additives compared to *** peroxidase enzyme(GPx)recorded the highest rates(P<0.05)in groups fed Artemia supplemented with DHAS and FOVE followed by FOCE and *** the lowest record for GPx activity was noted in the FO *** conclusion,Using augmented Artemia nauplii with fish oil+vitamin E in single or in companion with vitamin C as cheap antioxidants support *** larval growth,survival,and antioxidant efficacy during the critical weaning period.

关键词： European seabass Gastrointestinal histology Antioxidants Vitamin C Vitamin E DHA

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