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Candidate genes conferring ethylene-response in cultivated peanuts determined by BSA-seq and fine-mapping

The Crop Journal 2024年第3期12卷 856-865页

作者： Yanyan Tang Zhong Huang Shaohui Xu Wenjie Zhou Jianjun Ren Fuxin Yu Jingshan Wang Wujun Ma Lixian Qiao College of Agronomy Qingdao Agricultural UniversityQingdao 266109ShandongChina Murdoch University Food Futures InstitutePerthWA 6150Australia The Characteristic Laboratory of Crop Germplasm Innovation and Application Provincial Department of EducationCollege of AgronomyQingdao Agricultural UniversityQingdao 266109ShandongChina Academy of Dongying Efficient agricultural Technology and industry on Saline and Alkaline Land in Collaboration with Qingdao agricultural University Dongying 257347ShandongChina

Ethylene plays essential roles in plant growth,development and stress *** ethylene signaling pathway and molecular mechanism have been studied extensively in Arabidopsis and rice but limited in ***,we established a sand-culture method to screen pingyangmycin mutagenized peanut lines based on their specific response to ethylene(“triple response”).An ethylene-insensitive mutant,inhibition of peanut hypocotyl elongation 1(iph1),was identified that showed reduced sensitivity to ethylene in both hypocotyl elongation and root *** bulked segregant analysis sequencing,a major gene related to iph1,named AhIPH1,was preliminarily mapped at the chromosome Arahy.01,and further narrowed to a 450-kb genomic region through substitution mapping strategy.A total of 7014 genes were differentially expressed among the ACC treatment through RNA-seq analysis,of which only the Arahy.5BLU0Q gene in the candidate mapping interval was differentially expressed between WT and mutant *** sequence variations,functional annotation and transcriptome analysis revealed that a predicated gene,Arahy.5BLU0Q,encoding SNF1 protein kinase,may be the candidate gene for *** gene contained two single-nucleotide polymorphisms at promoter region and was more highly expressed in iph1 than *** findings reveal a novel ethylene-responsive gene,which provides a theoretical foundation and new genetic resources for the mechanism of ethylene signaling in peanuts.

关键词： Ethylene-insensitive Hypocotyl elongation AhIPH1 Candidate gene Genetic resources

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Multi-species probiotics enhance survival,growth,intestinal microbiota and disease resistance of rohu(Labeo rohita)larvae

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Water Biology and Security 2024年第1期3卷 71-79页

作者： Zannatul Ferdous Md Kabir Hossain Md Hadiuzzaman SMRafiquzzaman KM Abdul Halim Tanvir Rahman Md Ali Reza Faruk Zulhisyam Abdul Kari Md Shahjahan Laboratory of Fish Ecophysiology Department of Fisheries ManagementBangladesh Agricultural UniversityMymensingh2202Bangladesh department of Aquaculture Bangladesh Agricultural UniversityMymensingh2202Bangladesh department of Fisheries Matshoy BhabanDhakaBangladesh department of Fisheries Biology&Aquatic Environment Bangabandhu Sheikh Mujibur Rahman Agricultural UniversityGazipur1706Bangladesh department of agricultural Sciences Faculty of Agro-Based IndustryUniversiti Malaysia KelantanJeli Campus17600JeliKelantanMalaysia

Multi-strain probiotics provide the most dependable approaches to improve health,immune response,and disease resistance in farmed *** the present study,we examined the effects of multi-species probiotics on the survival,growth,immune response,and disease resistance of rohu(Labeo rohita)*** hatched larvae from the day of first feeding(average weight of 0.003 g)were reared with multi-species probiotics having a combination of Bacillus subtilis(109 colony forming units(cfu)/mL)and Lactobacillus spp.(Lactobacillus plantarum,Lactobacillus buchneri-1011 cfu/mL)in water containing doses of 0(control-C),0.5 mL/L(treatment 1-T1),and 1.0 mL/L(treatment 2-T2)in triplicates for 90 *** the experiment,a challenge test was performed to assess the fish's resistance to pathogenic Aeromonas *** higher survival was recorded in larvae of treated groups(87%in T2 and 79%in T1)compared to the control(62%).Significantly higher growth performance(weight gain and specific growth rate—SGR)was shown by the probiotic-treated larval groups compared to the *** supplementation resulted in significantly higher counts of total viable colony(TVC)and lactic acid bacteria(LAB)in the *** immunological parameters(mucosal fold fattening,goblet cell abundance,expansion of lamina propria and enterocytes)of the gut were significantly better in probiotic-treated *** liver of treated fish showed irregular shape nuclei turning into regular shape and reducing spaces between the hepatic ***-treated fish had the highest post-challenge survival rate(90%)against *** *** erythrocytes of challenged fish treated with probiotics had significantly lower frequencies of various nuclear and cellular *** findings suggest that multi-species probiotic supplements could improve the survival,growth,health status,and immune response of rohu in the early stages of its development.

关键词： Aquaculture Disease resistance Immunity Labeo rohita Probiotics

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An efficient and universal protoplast-based transient gene expression system for genome editing in Brassica crops

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Horticultural Plant Journal 2024年第4期10卷 983-994页

作者： Xiaoxiao Yu Jintai Yu Yin Lu Wenjing Li Guanzhong Huo Jun Zhang Yan Li Jianjun Zhao Jun Li State Key Laboratory of North China Crop Improvement and Regulation College of Life SciencesHebei Agricultural UniversityBaodingHebei 071001China Hebei Key Laboratory of Plant Physiology and Molecular Pathology Hebei Agricultural UniversityBaodingHebei 071001China Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei Collaborative Innovation Center of Vegetable Industry in HebeiCollege of HorticultureHebei Agricultural UniversityBaodingHebei 071001China College of Modern Science and Technology Hebei Agricultural UniversityBaodingHebei 071001China Human Resource department Hebei Agricultural UniversityBaodingHebei 071001China

Protoplast-based transient gene expression system has been widely used in plant genome editing because of its simple operation and less *** order to establish a universal protoplast-based transient transfection system for verifying activities of genome editing vectors containing targets in Brassica,we systematically optimized factors affecting protoplast isolation and transient gene *** established an efficient protoplast-based transient gene expression system(PTGE)in Chinese cabbage,achieving high protoplast yield of 4.9×10^(5)·g^(-1)FW,viability over 95%,and transfection efficiency of 76%.We showed for the first time that pretreatment of protoplasts with a hypotonic MMG could significantly enhance the transfection ***,protoplasts incubated at 37℃ for 6 min improved the transfection efficiency to 86%.We also demonstrated that PTGE worked well(more than 50%transfection efficiency)in multiple Brassica species including cabbage,Pak Choi,Chinese kale,and ***,PTGE was used for validating the activities of CRISPR/Cas9 vectors containing targets in Chinese cabbage,cabbage,and pak choi,demonstrating the broad applicability of the established PTGE for genome editing in Brassica crops.

关键词： Chinese cabbage PTGE Transfection efficiency CRISPR/Cas9

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Efficient DIPA-CRISPR-mediated knockout of an eye pigment gene in the white-backed planthopper,Sogatella furcifera

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Insect Science 2024年第4期31卷 1015-1025页

作者： Meng-Qi Zhang Lang-Lang Gong Ya-Qin Zhao Yun-Feng Ma Gui-Jun Long Huan Guo Xuan-Zheng Liu J.Joe Hull Youssef Dewer Chao Yang Ning-Ning Zhang Ming He Peng He National Key Laboratory of Green Pesticides Key Laboratory of Green Pesticide and Agricultural BioengineeringMinistry of EducationCenter for R&D of Fine Chemicals of Guizhou UniversityGuiyangChina Pest Management and Biocontrol Research Unit US Arid Land Agricultural Research CenterUSDA Agricultural Research ServicesMaricopaArizonaUSA Phytotoxicity Research department Central Agricultural Pesticide LaboratoryAgricultural Research CenterDokkiGizaEgypt Guizhou Jifeng Seed industry Limited Liability Company XingyiGuizhou ProvinceChina Shandong Facility Horticulture Bioengineering Research Center Weifang University of Science and TechnologyWeifangShandong ProvinceChina

Although CRISPR/Cas9 has been widely used in insect gene editing,the need for the microinjection of preblastoderm embryos can preclude the technique being used in insect species with eggs that are small,have hard shells,and/or are difficult to collect and maintain outside of their normal *** is the case with Sogatella furcifera,the white-backed planthopper(WBPH),a significant pest of Oryza sativa(rice)that oviposits inside rice *** extraction from the stem runs the risk of mechanical damage and hatching is heavily influenced by the micro-environment of the rice *** bypass these issues,we targeted embryos prior to oviposition via direct parental(DIPA)-CRISPR,in which Cas9 and single-guide RNAs(sgRNAs)for the WBPH eye pigment gene tryptophan 2,3-dioxygenase were injected into the hemocoel of adult *** at varying numbers of days posteclosion were evaluated to determine at what stage their oocyte might be most capable of taking up the gene-editing *** evaluation of the offspring indicated that the highest G0 gene-edited efficacy(56.7%)occurred in females injected 2 d posteclosion,and that those mutations were heritably transmitted to the G1 *** study demonstrates the potential utility of DIPA-CRISPR for future gene-editing studies in non-model insect species and can facilitate the development of novel pest management applications.

关键词： CRISPR/Cas9 DIPA-CRISPR gene editing tryptophan 2,3-dioxygenase WBPH

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Distribution of antioxidants and phenolic compounds in flour milling fractions from hard red winter wheat

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Grain & Oil Science and Technology 2024年第2期7卷 71-78页

作者： Lauren Renee Brewer Jittawan Kubola Sirithon Siriamornpun Yong-Cheng Shi department of Grain Science and industry Kansas State UniversityManhattanKS 66506USA department of Food Innovation and Processing Faculty of Agricultural TechnologyBuriram Rajabhat UniversityBuriram 31000Thailand Research Unit of Thai Food innovation Department of Food Technology and NutritionMahasarakham UniversityMahasarakham 44000Thailand

Mature wheat kernels contain three main parts:endosperm,bran,and *** milling results in multiple streams that are chemically different;however,the distribution of antioxidants and phenolic compounds has not been well documented in terms of conventional milling by-product *** this study,multiple analytical methods were used to investigate antioxidant activity and phenolic compound compositions of hard red winter wheat(whole ground wheat),the parts of a wheat kernel(bran,flour,germ),and wheat by-product streams(mill feed,red dog,shorts)for the first *** each mill stream,phenolic compounds(total,flavonoid,and anthocyanin contents)were determined and antioxidant activities were evaluated with 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical-scavenging activity,ferric reducing/antioxidant power(FRAP),and total antioxidant capacity *** differences(P<0.05)were observed in phenolic concentrations among fractions of bran,flour,and germ milled from the same kernels and noted that germ accounts for the majority of antioxidant properties,whereas bran contains a substantial portion of phenolic compounds and *** feed was high in phenolic content(5.29 mg FAE/g),total antioxidant capacity(866 mg/g),and antioxidant activity(up to 75% DPPH inhibition and 20.26μmol FeSO_(4)/g).The comprehensive information on distribution of antioxidants and phenolic compounds provides insights for future human consumption of commonly produced co-products from flour milling,and for selecting and using different milling fractions to make foods with improved nutritional properties.

关键词： Wheat Milling streams Antioxidants Phenolic acids Flavonoid Anthocyanin

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Optimization Mechanism of Mechanical Properties of Basalt Fiber-Epoxy Resin Composites by Interfacially Enriched Distribution of Nano-Starch Crystals

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Chinese Journal of Mechanical Engineering 2024年第3期37卷 289-296页

作者： Yanpeng Wei Jiale Zhao Jian Zhuang Peng Zhang Zhiwu Han College of Biological and agricultural Engineering Jilin UniversityChangchun 130025China Key Laboratory of Bionics Engineering Ministry of EducationJilin UniversityChangchun 130025China Weichai Lovol Heavy industry Co. LTDWeifang 261031China

Fibre reinforced polymer composites have become a new generation of structural materials due to their unique advantages such as high specific strength,designability,good dimensional stability and ease of large-area monolithic ***,the problem of interfacial bonding between the resin matrix and the fibres limits the direct use of reinforcing fibres and has become a central difficulty in the development of basalt fibre-epoxy *** paper proposes a solution for enhancing the strength of the fibre-resin interface using maize starch nanocrystals,which are highly yield and ***,in this paper,corn starch nanocrystals(SNC)were prepared by hydrolysis,and were deposited on the surface of basalt fibers by electrostatic *** that,in order to maximize the modification effect of nano-starch crystals on the interface,the basalt fiber-epoxy resin composite samples were prepared by mixing in a pressureless molding *** test results shown that the addition of basalt fibers alone led to a reduction in the strength of the *** of 0.1 wt%SNC on the surface of basalt fibers can make the strength consistent with pure epoxy *** the adsorption amount of SNC reached 0.5 wt%,the tensile strength of the samples was 23.7%higher than that of pure epoxy *** is due to the formation of ether bond homopolymers between the SNC at the fibre-epoxy interface and the epoxy resin,which distorts the originally smooth interface,leading to increased stress concentration and the development of *** enhances the binding of basalt *** conclusions of this paper can provide an effective,simple,low-cost and non-polluting method of interfacial enhancement modification.

关键词： Basalt fibres Epoxy resin Fibre reinforced composites Starch nanocrystals Eco-friendly

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Quantitative and Qualitative Responses of Hydroponic Tomato Production to Different Levels of Salinity

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Phyton-International Journal of Experimental Botany 2024年第6期93卷 1311-1323页

作者： Khalid A.Al-Gaadi Ahmed M.Zeyada ElKamil Tola Abdullah M.Alhamdan Khalid A.M.Ahmed Rangaswamy Madugundu Mohamed K.Edrris department of agricultural Engineering College of Food and Agriculture SciencesKing Saud UniversityRiyadh11451Saudi Arabia Precision Agriculture Research Chair Deanship of Scientific ResearchKing Saud UniversityRiyadh11451Saudi Arabia Chair of Dates industry&Technology Deanship of Scientific ResearchKing Saud UniversityRiyadh11451Saudi Arabia agricultural Research Centre Agricultural Engineering Research Institute(AEnRI)Giza12619Egypt

From economic and nutritional points of view,tomato is,historically,considered one of the most important *** signiﬁcant yield reduction,most commercial cultivars of tomato crops are sensitive to moderate levels of ***,high levels of salt stress can negatively affect the yield and quality of tomato ***,this study was conducted to evaluate the yield and fruit quality of three tomato cultivars(Forester,Ghandowra-F1,and Feisty-Red)cultivated hydroponically,under three different levels of nutrient solution *** of tomato fruits was performed based on quantity(number and weight of fruits,and total fruit yield),physical quality(color andﬁrmness),and chemical quality(refractometric index“Brix”,pH,EC,K^(+),Na^(+),and NO_(3)-).Experiments were conducted using three salinity levels of the nutrient solution with electrical conductivity values(dSm^(-1))of 2.5(control),6.0,and *** showed that the studied tomato cultivars were signiﬁcantly inﬂuenced by high salinity(9.5 dSm^(-1))in comparison to the low(2.5 dSm^(-1))and medium(6.0 dSm^(-1))levels of *** average,the highest fruit weight per plant of 1944.84 g and total fruit yield of 4.42 kgm^(-2) were observed at the low salinity level;however,no signiﬁcant differences were obtained in the two yield factors(single fruit weight and total fruit yield)for the low and medium salinity *** the other hand,a signiﬁcant reduction in tomato yield(31%)was associated with the high salinity level compared to the yield at low and medium salinity *** of physical quality parameters showed highly signiﬁcant differences among all salinity *** average,the maximum value of color change(1.72)was associated with the medium salinity level,and the maximum value ofﬁrmness(9.61 Ncm^(-1))was recorded at the high salinity *** levels and tomato cultivars introduced signiﬁcant differences in chemical quality parameters;however,no signiﬁcant differences in these

关键词： Quality cultivars yield color ﬁrmness brix

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Pelleting and particle size reduction of corn increase net energy and digestibility of fiber,protein,and fat in corn-soybean meal diets fed to group-housed pigs

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Journal of Animal Science and Biotechnology 2024年第4期15卷 1751-1760页

作者： Su A Lee Diego A.Rodriguez Chad B.Paulk Hans H.Stein department of Animal Sciences University of IllinoisUrbanaIL 61801USA department of Grain Sciences and industry Kansas State UniversityManhattanKS 66506USA

Background Reduction of the particle size of corn increases energy digestibility and concentrations of digestible and metabolizable *** may also reduce particle size of grain,but it is not known if there are interactions between particle size reduction and *** objective of this experiment was to test the hypothesis that particle size reduction and pelleting,separately or in combination,increase N balance,apparent total tract digestibility(ATTD)of fiber and fat,and net energy(NE)in corn-soybean meal diets fed to group-housed *** Six corn-soybean meal-based diets were used in a 3×2 factorial design with 3 particle sizes of corn(i.e.,700,500,or 300μm)and 2 diet forms(i.e.,meal or pelleted).Pigs were allowed ad libitum access to feed and ***-four castrated male pigs(initial weight:29.52 kg;standard diviation:1.40)were allotted to the 6 diets using a 6×6 Latin square design with 6 calorimeter chambers(i.e.,4 pigs/chamber)and 6 *** consumption and CO_(2)and CH_(4)productions were measured during fed and fasting states and fecal and urine samples were *** Regardless of particle size of corn,the ATTD of gross energy(GE),N,and acid-hydrolyzed ether extract(AEE),and the concentration of NE were greater(P<0.05)in pelleted diets than in meal *** of diet form,the ATTD of GE,N,and AEE,and the concentration of NE were increased(linear;P<0.05)by reducing the particle size of corn,but the increase was greater in meal diets than in pelleted diets(interaction;P<0.05).Conclusions Both pelleting and reduction of corn particle size increased nutrient digestibility and NE,but increases were greater in meal diets than in pelleted diets.

关键词： Corn Digestibility Feed technology Net energy Particle size Pelleting

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Risk Constrained Self-scheduling of AA-CAES Facilities in Electricity and Heat Markets:A Distributionally Robust Optimization Approach

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CSEE Journal of Power and Energy Systems 2024年第3期10卷 1159-1167页

作者： Zhiao Li Laijun Chen Wei Wei Shengwei Mei department of Electrical Engineering Tsinghua UniversityBeijing 100084China New Energy(Photovoltaic)industry Research Center Qinghai UniversityXining 810016China IEEE

Advanced adiabatic compressed air energy storage(AA-CAES)has the advantages of large capacity,long service time,combined heat and power generation(CHP),and does not consume fossil fuels,making it a promising storage technology in a low-carbon *** appropriate self-scheduling model can guarantee AA-CAES’s profit and attract ***,very few studies refer to the cogeneration ability of AA-CAES,which enables the possibility to trade in the electricity and heat markets at the same *** this paper,we propose a multimarket self-scheduling model to make full use of heat produced in *** volatile market price is modeled by a set of inexact distributions based on historical data through-***,the self-scheduling model is cast as a robust risk constrained program by introducing Stackelberg game theory,and equivalently reformulated as a mixed-integer linear program(MILP).The numerical simulation results validate the proposed method and demonstrate that participating in multienergy markets increases overall *** impact of uncertainty parameters is also discussed in the sensibility analysis.

关键词： Advanced adiabatic compressed air energy storage(AA-CAES) conditional value at risk(CVaR) distributionally robust optimization(DRO) heat market self-scheduling Stackelberg game

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The effects of mixed prebiotics in aquaculture:A review

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Aquaculture and Fisheries 2024年第1期9卷 28-34页

作者： Wendy Wee Noor Khalidah Abdul Hamid Khairiyah Mat Raja Ili Airina Raja Khalif Nor Dini Rusli Mohammad Mijanur Rahman Muhammad Anamul Kabir Lee Seong Wei Centre for Foundation and Continuing Education Universiti Malaysia Terengganu21030Kuala NerusTerengganuMalaysia School of Biological Sciences Universiti Sains Malaysia11800MindenPulau PinangMalaysia department of agricultural Sciences Faculty of Agro-Based IndustryUniversiti Malaysia KelantanJeli Campus17600JeliKelantanMalaysia Faculty of Fisheries Sylhet Agricultural UniversitySylhet3100Bangladesh

Aquaculture industry contributes significant aquaculture species production to meet fish protein demand from the *** industry is gearing towards intensification to increase more production in the near *** technologies and efforts were introduced into aquaculture industry to bring it up to the next higher ***,the development of aquaculture industry is hindered by disease *** a result,excessive use of antibiotics and chemicals were applied to reduce or minimize risk of disease outbreak in aquaculture farming ***,aquacultures products and environment were highly exposed to these antibiotics and *** resolve this issue,fish farmers were encouraged to utilize alternative method in aquaculture species health *** has become a new approach in aquaculture industry to maintain aquaculture species health and a good environment at the same time produce chemical and antibiotic-free aquaculture *** this review paper,we summarize and discuss the effects of prebiotic mixtures administration on growth performance,immune response,disease resistance,and tolerance against abiotic stressors of aquatic animals.

关键词： Growth performance Immunosaccharide Disease resistance Stress relieve

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