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Characterization of the infectivity of an Indonesian Zika virus strain in mammalian cell lines

Asian Pacific Journal of Tropical Biomedicine 2024年第5期14卷 215-224页

作者： Justus Thomas Obiajulu Sievers Anom Bowolaksono R.Tedjo Sasmono Graduate School of biology Department of BiologyFaculty of Mathematics and Natural SciencesUniversitas IndonesiaDepok 16424Indonesia Eijkman Research Center for Molecular biology National Research and Innovation AgencyCibinong 16911Indonesia Cellular and Molecular Mechanisms in Biological System Research Group Department of BiologyFaculty of Mathematics and Natural SciencesUniversitas IndonesiaDepok 16424Indonesia

Objective:To characterize the infection patterns and growth characteristics of the Zika virus(ZIKV)strain JMB-185 from Indonesia in various mammalian cell ***:ZIKV was grown in human(A549,HEK293,HepG2,Huh7,Jurkat,and THP-1)and non-human mammalian(RAW264.7,Vero,and Vero76)cell *** replication kinetics were measured using plaque assay,while intra-and extracellular viral RNA concentrations were assessed using *** cytometry was used to quantify the infected cells and cell viability was measured using an MTT *** ability of ZIKV to infect cell lines was visualized using a fluorescence immunostaining ***:This ZIKV strain preferentially infected the lung,kidney,and liver cell lines A549,HEK293,Huh7,Vero,and Vero76,but not the immune cells Jurkat,RAW264.7,and *** contrast,the ZIKV showed no sign of infection in HepG2 cells,while maintaining viral titer over 3 days post-infection,with no infection recorded in immunostaining,no increase in viral RNA,and no indication of cell ***:The Indonesian ZIKV strain has a similar infection profile as other strains,except for its poor infectivity on HepG2 *** on the growth characteristics of Indonesia ZIKV will help expand our understanding of the biology of ZIKV which will be useful for various applications including antiviral discovery.

关键词： Zika virus Infectivity Replication Cell lines In vitro

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Use of induced pluripotent stem cell-derived brain cells,organoids,assembloids,and blood-brain barrier models in understanding alcohol and anesthetic-induced brain injuries:an emerging perspective

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Neural Regeneration Research 2024年第5期19卷 953-954页

作者： Xiaowen Bai department of Cell biology Neurobiology&AnatomyMedical College of WisconsinMilwaukeeWIUSA

Neurological disorders,including developmental disorders,Alzheimer’s disease(AD),and psychiatric conditions,have significant social and economic impacts *** extensive research into the underlying mechanisms of these disorders,effective treatments remain elusive,partly due to the complexity of the brain,the limited availability of human brain tissue,and the blood-brain barrier(BBB)’s impermeability to certain *** perspective article discusses the potential of human induced pluripotent stem cell(iPSC)-based models of brain cells,organoids,assembloids,and BBB to advance our understanding of the etiology,progression,and mechanisms of brain injuries induced by alcohol consumption and general *** models could also be used to develop protective and therapeutic approaches.

关键词： injuries protective drugs

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Metallothionein 1E Alleviates Cadmium-induced Renal Cytotoxicity through Promoting Mitochondrial Functional Recovery

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Biomedical and Environmental Sciences 2024年第1期37卷 117-121页

作者： WEN Si Hui LI Lu Bei HUANG Hui Dan XIE Ying LUO Lei Key Laboratory of Molecular Epidemiology of Hunan Province School of MedicineHunan Normal UniversityChangsha 410081HunanChina department of Occupational Health Changsha Centre for Disease Control and PreventionChangsha 410004HunanChina The Key Laboratory of Model Animals and stem Cell biology in Hunan Province Hunan Normal University School of MedicineChangsha 410013HunanChina

The pervasive utilization of industrial substances has escalated human exposure to cadmium(Cd),a metal associated with long-term negative health outcomes such as renal dysfunction,neurological disorders,and various cancers^([1]).Once ingested by humans,Cd interacts with cysteine-rich metallothioneins(MTs)which have metal-binding and antioxidant properties and is subsequently transported to the kidney^([2]).

关键词： Renal kidney cadmium

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Recent clinical trials and optical control as a potential strategy to develop microtubule-targeting drugs in colorectal cancer management

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World Journal of Gastroenterology 2024年第13期30卷 1780-1790页

作者： Katsuhiro Kita Allen Burdowski department of biology St.Francis CollegeBrooklynNY 11201United States

Colorectal cancer(CRC)has remained the second and the third leading cause of cancer-related death worldwide and in the United States,*** significant improvement in overall survival has been achieved,death in adult populations under the age of 55 appears to have increased in the past *** new classes of therapeutic strategies such as immunotherapy have emerged,their application is very limited in CRC so ***(MT)inhibitors such as taxanes,are not generally successful in *** may be some way to make MT inhibitors work effectively in *** potential advantage that we can take to treat CRC may be the combination of optical techniques coupled to an endoscope or other fiber optics-based devices.A combination of optical devices and photo-activatable drugs may allow us to locally target advanced CRC cells with highly potent MT-targeting *** this Editorial review,we would like to discuss the potential of optogenetic approaches in CRC management.

关键词： Colorectal cancer Chemotherapy Microtubule Combretastatin Photopharmacology

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Identification and functional characterization of fish IL‑17 receptors suggest important roles in the response to nodavirus infection

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Marine Life Science & Technology 2024年第2期6卷 252-265页

作者： Carmen González‑Fernández Miguel A.García‑Álvarez Alberto Cuesta department of Cell biology and Histology Faculty of BiologyUniversity of Murcia30100 MurciaSpain Laboratoire d’écotoxicologie Centre de Lyon-VilleurbanneINRAEUR RiverLy69625 VilleurbanneFrance

Th17 is a lymphocyte T helper(Th)subpopulation relevant in the control and regulation of the immune response characterized by the production of interleukin(IL)-*** crucial cytokine family acts through their binding to the IL-17 receptors(IL-17R),having up to six *** the biology of fish Th17 is well-recognized,the molecular and functional characterization of IL-17 and IL-17R has been ***,our aim was to identify and characterize the IL-17R repertory and regulation in the two main Mediterranean cultured fish species,the gilthead seabream(Sparus aurata)and the European sea bass(Dicentrarchus labrax).Our in silico results showed the clear identification of six members in each fish species,from IL-17RA to IL-17RE-like,with well-conserved gene structure and protein domains with their human *** of them showed wide and constitutive transcription in naïve tissues but with highest levels in mucosal tissues,namely skin,gill or *** leucocytes,T mitogens showed the strongest up-regulation in most of the il17 receptors though il17ra resulted in inhibition by most ***,in vivo nodavirus infection resulted in alterations on the transcription of il17 *** nodavirus infection led to some increments in the il17ra,il17rb,il17rc and il17rd transcripts in the susceptible European sea bass,many down-regulations were observed in the resistant gilthead *** data identify the presence and conservation of six coding IL-17R in gilthead seabream and European sea bass as well as their differential regulation in vitro and upon nodavirus infection.

关键词： Th17 cells IL-17 receptors Immunity Teleost fish Nodavirus(NNV) Aquaculture

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A Vertex Network Model of Arabidopsis Leaf Growth

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Communications on Applied Mathematics and Computation 2024年第1期6卷 454-488页

作者： Luke Andrejek Janet Best Ching-Shan Chou Aman Husbands department of Mathematics The Ohio State UniversityColumbusOHUSA department of biology The University of PennsylvaniaPhiladelphiaPAUSA

biology provides many examples of complex systems whose properties allow organisms to develop in a highly reproducible,or robust,*** such system is the growth and development of flat leaves in Arabidopsis *** mechanistically challenging process results from multiple inputs including gene interactions,cellular geometry,growth rates,and coordinated cell *** better understand how this complex genetic and cellular information controls leaf growth,we developed a mathematical model of flat leaf *** two-dimensional model describes the gene interactions in a vertex network of cells which grow and divide according to physical forces and genetic ***,the model predicts the presence of an unknown additional factor required for the formation of biologically realistic gene expression domains and iterative cell *** two-dimensional model will form the basis for future studies into robustness of adaxial-abaxial patterning.

关键词： Robustness Adaxial-abaxial patterning Mathematical modeling Gene regulatory networks(GRNs) Transcription factors Small RNAs

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Luminous fishes:Endocrine and neuronal regulation of bioluminescence

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Aquaculture and Fisheries 2024年第3期9卷 486-500页

作者： José Paitio Yuichi Oba department of Environmental biology Chubu UniversityKasugai487-8501Japan

Bioluminescence is the ability of organisms to emit their own light which can be self-produced in light organs by photogenic cells(photocytes)or symbiotically by bearing cultures of luminous *** animals are mainly comprised in the ocean,nocturnal coastal shallow-water species or,as the majority of species,inhabiting deep-sea where dim light conditions are optimal for communication by luminous *** to difficult access to species,fisheries of bioluminescent fishes worldwide are rare,mostly for traditional cuisine and fishing *** are the only bioluminescent vertebrates and thus their light organs present an astonishing level of complexity for light modulation to adequate light signals for specific ecological *** control the output of light intensity using different mechanisms which can be categorized as being under control of hormonal or neuronal ***,neural control allows fast and direct response to the photocytes while endocrinal systems allow to control the light produced by bacteria,via manipulation of individual *** some species,mechanical movement of melanophore-filled tissues by muscular action is under neuronal or hormonal *** dimorphism of light organs,driven by hormonal regulation during ontogeny,takes a crucial role in communication and reproductive success of these species.

关键词： Bioluminescence Fish Hormonal Neural Endocrinological Control

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Identification of druggable host dependency factors shared by multiple SARS-CoV-2 variants of concern

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Journal of Molecular Cell biology 2024年第3期16卷 9-23页

作者： Ilaria Frasson Linda Diamante Manuela Zangrossi Elena Carbognin Anna Dalla Pietà Alessandro Penna Antonio Rosato Ranieri Verin Filippo Torrigiani Cristiano Salata Marìa Paula Dizanzo Lorenzo Vaccaro Davide Cacchiarelli Sara N.Richter Marco Montagner Graziano Martello department of Molecular Medicine University of PaduaPadua 35121Italy department of biology Armenise/Harvard Pluripotent Stem Cell Biology LaboratoryUniversity of PaduaPadua 35131Italy department of Surgery Oncology and GastroenterologyUniversity of PaduaPadua 35128Italy Veneto Institute of Oncology IOV-IRCCS Padua 35128Italy department of Comparative Biomedicine and Food Science University of PaduaPadua 35020Italy Telethon Institute of Genetics and Medicine(TIGEM) Armenise/Harvard Laboratory of Integrative GenomicsPozzuoli 80078Italy department of Translational Medicine University of Naples Federico IINaples 80138Italy School for Advanced Studies Genomics and Experimental Medicine ProgramUniversity of Naples Federico IINaples 80138Italy Microbiology and Virology Unit Padua University HospitalPadua 35128Italy

The high mutation rate of SARS-CoV-2 leads to the emergence of multiple variants,some of which are resistant to vaccines and drugs targeting viral *** host dependency factors,*** proteins required for viral replication,would help prevent the development of ***,it remains unclear whether different SARS-CoV-2 variants induce conserved cellular responses and exploit the same core host *** this end,we compared three variants of concern and found that the host transcriptional response was conserved,differing only in kinetics and *** regularly interspaced short palindromic repeats screening identified host genes required for each variant during *** of the genes were shared by multiple *** validated our hits with small molecules and repurposed the US Food and Drug Administration-approved *** the drugs were highly active against all the tested variants,including new variants that emerged during the study(Delta and Omicron).Mechanistically,we identified reactive oxygen species production as a key step in early viral *** such as N-acetyl cysteine(NAC)were effective against all the variants in both human lung cells and a humanized mouse *** study supports the use of available antioxidant drugs,such as NAC,as a general and effective anti-COVID-19 approach.

关键词： SARS-CoV-2 variants of concern host dependency factors antivirals N-acetyl cysteine

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Expression levels of K_(ATP)channel subunits and morphological changes in the mouse liver after exposure to radiation

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World Journal of Experimental Medicine 2024年第2期14卷 103-113页

作者： Ming Zhou Tao-Sheng Li Hiroshi Abe Hideo Akashi Ryoji Suzuki Yoshio Bando department of Anatomy Akita University Graduate School of MedicineAkita 010-8543Japan department of stem Cell biology Atomic Bomb Disease InstituteNagasaki UniversityNagasaki 852-8523Japan Sendai Old Age Refresh Station A Long-term Care Health FacilitySendai 981-1105Japan

BACKGROUND ATP sensitive K+(K_(ATP))channels are ubiquitously distributed in various of cells and tissues,including the *** play a role in the pathogenesis of myocardial and liver *** To evaluate the radiation-induced changes in the expression of K_(ATP)channel subunits in the mouse liver to understand the potential role of K_(ATP)channels in radiation *** Adult C57BL/6 mice were randomly exposed toγ-rays at 0 Gy(control,n=2),0.2 Gy(n=6),1 Gy(n=6),or 5 Gy(n=6).The livers were removed 3 and 24 h after radiation *** and eosin staining was used for morphological observation;immunohistochemical staining was applied to determine the expression of K_(ATP)channel subunits in the liver *** Compared with the control group,the livers exposed to 0.2 Gyγ-ray showed an initial increase in the expression of Kir6.1 at 3 h,followed by recovery at 24 h after *** to a high dose of 5.0 Gy resulted in decreased expression of Kir6.1 and increased expression of SUR2B at 24 ***,the expression of Kir6.2,SUR1,or SUR2A had no remarkable changes at 3 and 24 h after exposure to any of these *** The expression levels of Kir6.1 and SUR2B in mouse liver changed differently in response to different radiation doses,suggesting a potential role for them in radiation-induced liver injury.

关键词： Radiation exposure ATP-sensitive K^(+)channel Mouse Liver

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Virus-like nanoparticle vaccines for inducing long-lasting immunity against infectious diseases

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National Science Review 2024年第4期11卷 13-15页

作者： Yi-Nan Zhang Sarah Auclair Jiang Zhu department of Integrative Structural and Computational biology The Scripps Research Institute department of Immunology and Microbiology The Scripps Research Institute

Prophylactic vaccines that can generate long-lasting protective immune responses are needed for many infectious diseases. Efficient vaccination requires vaccine antigens to be retained and presented to B cells in lymp...

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