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Pedosphere 2015年第3期25卷 428-437页

作者： Gurdeep KAUR Mondem Sudhakara REDDY department of biotechnology Thapar University

A two-year field study was conducted to test the effects of two phosphate-solubilizing bacteria(PSB), Pantoea cypripedii(PSB-3)and Pseudomonas plecoglossicida(PSB-5), inoculated singly or together with rock phosphate(RP) fertilization on maize and wheat cropping cycle by comparing with chemical P fertilizer(diammonium phosphate, DAP), mainly in the crop yield, soil fertility and economic returns. Inoculation of PSB together with RP fertilization increased the crop growth in terms of shoot height, shoot and root dry biomass, grain yield and total P uptake in both maize and wheat crops compared to the other treatments. Soil fertility in the context of available P, enzyme activities and PSB population in both maize and wheat crops was significantly improved with PSB inoculation together with RP fertilization compared to DAP treatment. The combined use of PSB inoculation and RP fertilization was more economical due to minimal cost and maximum returns. These results suggested that PSB inoculation along with RP fertilization would be an appropriate substitute for chemical phosphate fertilizer application in sustainable agriculture systems.

关键词： diammonium phosphate Pantoea cypripedii Pseudomonas plecoglossicida soil fertility sustainable agriculture

Influence of Low-Energy Ion Irradiation on Plasma Membrane Permeability of Cells

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Chinese Physics Letters 2000年第1期17卷 61-63页

作者： ZHANG Dong-Mei CUI Fu-Zhai SUN Su-Qin LIN You-Bo TIAN Min-Bo CHEN Guo-Qiang department of materials Science and Engineering Tsinghua UniversityBeijing 100084 Analysis Center Tsinghua UniversityBeijing 100084 department of Biological Science and biotechnology Tsinghua UniversityBeijing 100084

Effect of low-energy ion irradiation on plasma membrane permeability has been investigated by using electron spin resonance(ESR)spectroscopy of spin probe *** investigated system is plumule cells of wheat(Triticum aestivum L.)seeds implanted by 30 keV N^(+) *** spectra indicated that plasmalemma permeability is sensitive to low-energy ion *** irradiations with increasing Huences up to semi-lethal dose lead to gradual increase in plasmalemma permeability of the plumule *** possible factors relevant to the changes in membrane permeability are discussed in relation to the changes in the physical state and chemical nature of membranes.

关键词： technique. permeability irradiation

Variability in pod and seed traits of Pongamia pinnata Pierre ecotypes in North Karnataka, India

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Journal of Forestry Research 2016年第3期27卷 557-567页

作者： Vinod Kumar Patil G.R.Naik department of biotechnology Gulbarga University

An evaluation of 30 naturally available candidate-plus trees（CPTs） of Pongamia pinnata was carried out to elucidate the genetic variability and relationship of pod and seed traits and progeny growth traits to select the best planting material with higher productivity. Significant variations were observed in pod and seed characteristics,oil content, and during the progeny trial studies. The highest values were found in 100-pod weight（410.4 gm）and 100-seed weight（195.92 gm） and the phenotypic and genotypic variance was found maximum in 100 pod weight（4364.71 and 4289.93） and 100 seed weight（813.8 and768.93）, respectively. Estimates of the phenotypic coefficient of variance were higher than the genotypic coefficient of variance for all the pod and seed traits and progeny growth traits, which depict the predominant role of the environment. Approximately 99 % of broad-sense heritability was revealed in oil content, followed by 100-pod weight（98.28 %）, and canopy growth（96.93 %）. Pod thickness shows the highest genetic advance of 56.61 %,followed by pod width（53.43 %） and canopy growth（49.3 %）. Pod and seed traits have proven a positive correlation between each other and with progeny growth traits,except a few negative values. Hierarchical clustering by Ward＇s minimum variance cluster analysis showed phylogeographical patterns of genetic diversity. K-means clustering revealed that trees from different geographical regions were grouped together in a cluster where as trees from the same geographical area are placed in a different cluster. Seven Pongamia ecotypes（GRP-8, 9, 13, 14, 18,28, and 29） are found superior in traits, namely 100-pod weight, 100-seed weight, oil content, germination rate,plant height, canopy growth, and collar diameter. The traits100-pod weight and 100-seed weight are highly correlated with the progeny growth traits and even have higher heritability and genetic advantages.

关键词： Biodiesel Morphological diversity Pod andseed traits Pongamia pinnata Progeny trial

Differential fate of metabolism of a disperse dye by microorganisms Galactomyces geotrichum and Brevibacillus laterosporus and their consortium GG-BL

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Journal of Environmental Sciences 2012年第7期24卷 1295-1304页

作者： Tatoba R.Waghmode Mayur B.Kurade Anuradha N.Kagalkar Sanjay P.Govindwar department of Biochemistry Shivaji University department of biotechnology Shivaji University

The present work aims to evaluate Brown 3 REL degrading potential of developed microbial consortium GG-BL using two microbial cultures, Galactomyces geotrichum MTCC 1360 （GG） and BrevibaciUus laterosporus MTCC 2298 （BL）. Microbial consortium GG-BL showed 100% decolorization of a dye Brown 3 REL, while individually G. geotrichum MTCC 1360 and B. laterosporus MTCC 2298 showed 26% and 86% decolorization under aerobic condition （shaking） respectively. Measurements of biochemical oxygen demand （BOD）（76%） and chemical oxygen demand （COD）（68%） were done after decolorization by consortium GG-BL. No induction in activities of oxidoreductive enzymes found in G. geotrichum while B. laterosporus showed induction of veratryl alcohol oxidase, Nicotineamide adenine dinucleotide-dichlorophenol indophenol （NADH-DCIP） reductase and riboflavin reductase indicating their role in dye metabolism. Consortium GG-BL showed induction in the activities of laccase, veratryl alcohol oxidase, tyrosinase, NADH-DCIP reductase and riboflavin reductase. Two different sets of induced enzymes from G. geotrichum and B. laterosporus work together in consortium GG-BL resulting in faster degradation of dye. The degradation of Brown 3 REL was analyzed using high performance thin layer chromatography （HPTLC）, high performance liquid chromatography （HPLC）, Fourier transform infrared spectroscopy （FT-IR） and gas chromatography mass spectroscopy （GC-MS）. Phytotoxicity study revealed that metabolites formed after degradation was significantly less toxic in nature.

关键词： consortium GG-BL decolorization Brown 3 REL biochemical oxygen demand laccase biodegradation

Tannic acid and saponin for removing arsenic from brownfield soils: Mobilization, distribution and speciation

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Journal of Environmental Sciences 2014年第4期26卷 855-864页

作者： Zygmunt Mariusz Gusiatin department of Environmental biotechnology University of Warmia and Mazury

Plant biosurfactants were used for the first time to remove As and co-existing metals from brownfield soils. Tannic acid （TA）, a polyphenol, and saponin （SAP）, a glycoside were tested. The soil washing experiments were performed in batch conditions at constant biosurfactant concentration （3%）. Both biosurfactants differed in natural pH, surface tension, critical micelle concentration and content of functional groups. After a single washing, TA （pH 3.44） more efficiently mobilized As than SAP （pH 5.44）. When both biosurfactants were used at the same pH （SAP adjusted to 3.44）, arsenic mobilization was improved by triple washing. The process efficiency for TA and SAP was similar, and depending on the soil sample, ranged between 50%-64%. Arsenic mobilization by TA and SAP resulted mainly from decomposition of Fe arsenates, followed by Fe3＋ complexation with biosurfactants. Arsenic was efficiently released from reducible and partially from residual fractions. In all soils, As（V） was almost completely removed, whereas content of As（III） was decreased by 37%-73%. SAP and TA might be used potentially to remove As from contaminated soils.

关键词： arsenic tannic acid saponin distribution speciation soil washing

Spectral Insights into Microdynamics of Thermoresponsive Polymers from the Perspective of Two-dimensional Correlation Spectroscopy

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Chinese Journal of Polymer Science 2017年第6期35卷 700-712页

作者：孙胜童武培怡 State Key Laboratory for Modification of Chemical Fibers and Polymer materials College of Chemistry Chemical Engineering and Biotechnology Center for Advanced Low-Dimension Materials Donghua University State Key Laboratory of Molecular Engineering of Polymers Collaborative Innovation Center of Polymers and Polymer Composite Materials Department of Macromolecular Science and Laboratory for Advanced Materials Fudan University

Generalized two-dimensional correlation spectroscopy （2DCOS） and its derivate technique, perturbation correlation moving window （PCMW）, have found great potential in studying a series of physico-chemical phenomena in stimuli-responsive polymeric systems. By spreading peaks along a second dimension, 2DCOS can significantly enhance spectral resolution and discern the sequence of group dynamics applicable to various external perturbation-induced spectroscopic changes, especially in infrared （IR）, near-infrared （NIR） and Raman spectroscopy. On the basis of 2DCOS synchronous power spectra changing, PCMW proves to be a powerful tool to monitor complicated spectral variations and to find transition points and ranges. This article reviews the recent work of our research group in the application of 2DCOS and PCMW in thermoresponsive polymers, mainly focused on liquid crystalline polymers and lower critical solution temperature （LCST）-type polymers. Details of group motions and chain conformational changes upon temperature perturbation can thus be elucidated at the molecular level, which contribute to the understanding of their phase transition nature.

关键词： Thermoresponsive polymers Phase transition Two-dimensional correlation spectroscopy Perturbation correlation moving window

High-quality reference genes for quantifying the transcriptional responses of Oryza sativa L. (ssp. indica and japonica) to abiotic stress conditions

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Chinese Science Bulletin 2013年第16期58卷 1919-1930页

作者： MAKSUP Sarunyaporn SUPAIBULWATANA Kanyaratt SELVARAJ Gopalan department of biotechnology Faculty of Science Mahidol University Plant biotechnology Institute National Research Council of Canada

Rice (Oryza sativa L.) is important to food security and is also an excellent model plant for numerous cereal crops. A functional genomics study in rice includes characterization of the expression dynamics of genes by quantitative real-time PCR (qPCR) analysis; this is a significant key for developing rice varieties that perform well in the face of adverse climate change. The qPCR analysis requires the use of appropriate reference genes in order to make any quantitative interpretations meaningful. Here, the new potential reference genes were selected from a huge public database of rice microarray experiments. The expression stability of 14 candidates and 4 conventional reference genes was validated by geNorm PLUS and NormFinder software. Seven candidates are superior to the conventionally used reference genes in qPCR and three genes can be used reliably for quantitating the expression of genes involved in abiotic stress responses. These high-quality references EP (LOC_Os05g08980), HNR (LOC_Os01g71770), and TBC (LOC_Os09g34040) worked very well in three indica genotypes and one japonica genotype. One of indica genotypes including the Jasmine rice, KDML105 developed in Thailand for which no reference genes have been reported until now.

关键词：功能基因组学胁迫反应非生物水稻胁迫条件籼稻品质实时定量PCR

Promotional effects of copper doping on Ti-Ce-O_x for selective catalytic reduction of NO by NH_3 at low temperature

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Journal of Rare Earths 2016年第3期34卷 268-275页

作者：杨波沈岳松曾燕伟沈树宝祝社民 College of materials Science and Engineering Nanjing Tech University College of biotechnology and Pharmaceutical Engineering Nanjing Tech University

A series of copper-doped Ti-Ce-O_x complex oxide catalysts were synthesized by sol-gel method and evaluated for selective catalytic reduction of NO by NH_3 at low temperature. The promotional effect of copper doping on their structure, acidity and catalytic activity were investigated by means of Brumauer-Emmett-Teller（BET）, temperature-programmed reduction（H_2-TPR）, X-ray diffraction（XRD）, scanning electron microscopy（SEM）, temperature programmed desorption（NH_3-TPD） and pyridine adsorption infrared spectrum（Py-IR） technologies. Results showed that the copper additives could improve the low temperature catalytic performance for selective catalytic reduction of Ti-Ce-O_x catalyst and the NO conversion efficiency of Ti-Cu-Ce-O_x catalyst reached above 90% at 150-250 oC（Ti/Cu=4）. The introduction of copper could enhance the redox property of the Ti-Ce-O_x complex oxide catalyst, refine the particle size caused by lattice distortion and oxygen vacancy defect and enhance the acid amount of the Lewis acid site. Moreover, Ti-Cu-Ce-O_x complex oxide catalyst also had good anti-sulfur ability and anti-water influence, when injecting 300 ppm SO_2 and 10 vol.%H_2O, the NO conversion efficiency of Ti-Cu-Ce-O_x catalyst reached 80%.

关键词： Ti-Cu-Ce-Ox catalyst selective catalytic reduction low temperature Cu doping rare earths

Embryo culture is an efficient way to conserve a medicinally important endangered forest tree species Strychnos potatorum

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Journal of Forestry Research 2013年第2期24卷 279-283页

作者： Srikanth Kagithoju Vikram Godishala Madhusudhan Kairamkonda Rama Swamy Nanna department of biotechnology Kakatiya University

The present study reports a protocol for germination of Strychnos potatorum （ver. Tel. Chilla） using zygotic embryo culture as an embryo rescue method. A 100% germination rate was obtained by culturing the embryos on full-strength Murashige and Skoog＇s medium （MS） containing 20 g/L sucrose in comparison to McCown and Lloyd＇s Woody Plant Medium （WPM）. Germination rates decreased when the sucrose concentration was lower or higher than 20 g·L-1 . WPM/MS medium containing glucose at levels 30, 20, 15 g·L-1 showed a smaller percentage of germination and at quarter strength, WPM/MS medium with glucose did not respond. Multiple shoot formation was found at 1.0 2.0 mg/L BAP; 3.0 mg/L Kn; 2.0 mg/L TDZ on MS medium with 20 g·L-1 sucrose. Germination rates improved when the embryos were placed upright （vertically） in the medium. The in vitro germinated seedlings were acclimatized in a walk-in-chamber and maintained in the green house with the survival rate of 65% 75%. These plants were transferred to the field and were found to be phenotypically normal, healthy and similar to donor plants. This protocol will be useful to overcome seed dormancy and for rapid multiplication and conservation of S. potatorum using zygotic embryo culture.

关键词： endangered tree species Strychnos potatorum In vitro zygotic embryo culture immobilization of zygotic embryos embryo rescue

Synthesis of silver nanoparticles by Lactobaciluus acidophilus 01 strain and evaluation of its in vitro genomic DNA toxicity

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Nano-Micro Letters 2010年第3期2卷 160-163页

作者： S.Karthick Raja Namasivayam Gnanendra Kumar E Reepika R department of biotechnology Sathyabama University

Silver nanoparticles synthesized by dried biomass of Lactobaciluus acidophilus 01 strain was evaluated against toxicity of genomic DNA isolated from bacteria(***) fungi(Beauveria bassiana) algae(Seenedesmus acutus) and human blood adopting standard condition was discussed in the present *** silver nanoparticles were characterized by UV-Vis spectroscopy and *** UV-Vis spectroscopy revealed the formation of silver nanoparticles by yielding the typical silver plasmon absorption maxima at 430 nm and SEM micrograph indicates the uniform spherical particles within the size range of 45?60 *** energy dispersive X-ray spectroscopy(EDX) of the nanoparticle confirmed the presence of elemental silver signal as strong *** above synthesized silver nanoparticles didn't cause any toxic effect on all the tested genomic DNA at all tested concentrations which reveals nil genomic nanoparticles induced toxicity.

关键词： Lactobacillus acidophilus Silver nanoparticles Antibacterial activity Biosynthesis