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Upregulated genes in toll-like receptor (TLR) signaling pathway in periodontitis-affected gingival tissues

Open Journal of Stomatology 2014年第1期4卷 22-28页

作者： Daisuke Abe Takehiko Kubota Toshiya Morozumi Hiromasa Yoshie Division of periodontology Department of Oral Biological Science Niigata University Graduate School of Medical and Den-tal Sciences Niigata Japan

Toll-like receptor (TLR) signaling is thought to be one of the most important pathways initiating periodontitis onset. We have previously reported that the TLR signaling pathway is upregulated in periodontitis-affected gingival tissues by microarray pathway frequency analysis. The aim of the present study was to quantitatively analyze specific upregulated genes in the TLR signaling pathway, as compared to healthy controls. Healthy and periodontitis-affected gingival tissues were taken from distinct sites of 3 patients with severe chronic periodontitis. Total RNAs from 6 gingival tissue samples were used for microarray. Samples were taken from 14 chronic periodontitis patients and 14 healthy individuals for quantitative reverse transcription real-time polymerase chain reaction (qRT-PCR) analysis. Data-mining analyses, such as pathway analyses, were performed and significant biological pathways in periodontitis were identified. In addition, qRT-PCR analysis was performed for 5 genes—cluster of differentiation 14 (CD14), lymphocyte antigen 96 (MD-2), interleukin-1 beta (IL-1β), interleukin 8 (IL-8), and chemokine ligand 9 (CXCL-9), which are associated with TLR signaling, in order to confirm the results of pathway analysis. qRT-PCR verified that the transcripts for 5 genes in the TLR signaling pathway were significantly upregulated (MD-2 p = 0.0082, CD14 p = 0.0322, IL-1β p = 0.0126, IL-8 p = 0.0438, CXCL-9 p = 0.0325), which was consistent with pathway analyses. We confirmed upregulated MD-2 gene expression levels and associated TLR pathway gene expression, including CD14, IL-1β, IL-8 and CXCL-9, in periodontitis-affected gingival tissues, as compared with healthy controls.

关键词： TLR Signaling Pathway Gene Expression Microarray qRT-PCR

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Hyperbranched Azopolymer with Quadruple Responsibility

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Chinese Journal of Polymer Science 2021年第9期39卷 1169-1176页

作者： Chen Tian Ke-Cong Zhou Yu-Fei Lua Jin-Jie Li Yuan Yao Xin-Feng Tao Qi-Xin Zhuang Yu-Feng Xie Shao-Liang Ling Shanghai Key Laboratory of Advanced Polymeric Materials Key Laboratory for Ultrafine Materials of Ministry of EducationSchool of Materials Science and EngineeringEast China University of Science and TechnologyShanghai 200237China department of periodontology Shanghai Ninth People's HospitalCollege of StomatologyShanghai Key Laboratory of Stomatology&Shanghai Research Institute of StomatologyShanghai Jiao Tong UniversityShanghai 200011China

Simply constructing multiple responsive polymers with obvious shape and dimension variations on their assemblies upon different stimuli is still rarely *** this study,we report a hyperbranched polymer named HPAzoBAHB-star-PEG_(9) with quadruple-response to light,temperature,pH and oxidation *** polymer contains azobenzene chromophore,sulfide,amide and amine groups in its hydrophobic hyperbranched core,and the core is capped with hydrophilic polyethylene glycol(PEG_(9))***-5tar-PEG_(9) could assemble into unusual leaf-like lamellar micelles at 25℃ under the guidance of orderly arranged H-aggregate of azobenzene *** leaf-like lamellar micelles can transform into vesicles upon UV irradiation and lower temperature,or convert to smaller spherical micelles in acidic or oxidative environments,respectively,with the destroy of ordered azobenzene *** quadruple-responsive hyperbranched polymer is suitable to con struct multiple stimuli-resp on sive micro/nanostructures,or accurate delivery and release following subtle stimuli seque nces.

关键词： Hyperbranched Azopolymer Multiple responsibility Self-assembly

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Corrosion in Mg-alloy biomedical implants-the strategies to reduce the impact of the corrosion inflammatory reaction and microbial activity

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Journal of Magnesium and Alloys 2022年第12期10卷 3306-3326页

作者： Soumya Saha Widya Lestari Caroline Dini Murni Nazira Sarian Hendra Hermawan Valentim A.R.Barão Cortino Sukotjo Christos Takoudis department of Biomedical Engineering University of Illinois at ChicagoChicagoUnited States Fundamental Dental and Medical Sciences department Kulliyyah of DentistryInternational Islamic University MalaysiaKuantanMalaysia department of Prosthodontics and periodontology Piracicaba Dental SchoolUniversity of Campinas(UNICAMP)PiracicabaBrazil Institute of Systems Biology(INBIOSIS) Universiti Kebangsaan MalaysiaBandar Baru BangiSelangor 43600Malaysia department of Mining Metallurgical and Materials EngineeringLaval UniversityQuebec CityQC G1V 0A6Canada department of Restorative Dentistry University of Illinois at Chicago College of DentistryChicagoUnited States department of Chemical Engineering University of Illinois at ChicagoChicagoUnited States

The most common complication of orthopedic surgery is implant failure, which can result in catastrophic injury and a significant financial burden for patients. Implant failure can be caused by a variety of factors, the most common of which are peri–implant infection(or implantrelated infection), excessive inflammatory response which caused pain and aseptic loosening. Orthopedic surgeons now have a variety of options for treating these issues, including revision surgery, which has demonstrated to be effective. If excessive inflammatory reaction caused by the corrosion and peri–implant infection can be avoided, it will be of enormous social benefits. This review will provide a summary of corrosion and the inflammation reactions due to the corrosion and antimicrobial properties of Mg alloy-based implants covering both in vitro and in vivo studies. The strategies on hindering/overcoming the excessive inflammatory response and enhancing the antimicrobial activity are discussed in this review.

关键词： Mg-alloy implants review Corrosion and inflammation Microbial infection

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Effect of psoralen on alveolar bone resorption and inflammatory factors in rats with periodontitis

Effect of psoralen on alveolar bone resorption and inflammat...

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中华口腔医学会第十三次全国口腔粘膜病学暨第十一次全国口腔中西医结合学术大会

作者： Hua Liu Yingjie Xu Wenyi Zhang Jin Jing Ning Liu Wangchun Wang Meihua Gao Beibei Cong Yingtao Wu department of Stomatology School of Stomatology of Qingdao University department of periodontology Qingdao Stomatological Hospital Affiliated to Qingdao University

Fifty-two sprague dawley(SD) rats were randomized into control,periodontitis,psoralen and periodontitis + psoralen *** model was established by ligating bilateral maxillary second molars for 4 ***,rats were given normal saline or psoralen for another 4 weeks by gavage *** and eosin(HE) staining was used to detect the pathological *** levels of serum IL-10 and TNF-α were detected by *** periodontal tissues of bilateral maxillary molars were taken for Micro-CT detection to observe the changes of alveolar bone *** with control group,periodontitis rats had inflammatory cell infiltration in the connective tissue of the periodontal ligament,and the formation of periodontal pockets,and the surface of the alveolar bone was nibbling in periodontitis ***,compared with periodontitis group,the serum IL-10 level was significantly increased,but the serum TNF-α level was notably decreased in the periodontitis + psoralen group(P<0.05).Moreover,alveolar bone resorption value in the periodontitis +psoralen group was markedly decreased than that in periodontitis group(P<0.01).Psoralen can increase the serum IL-10 level,reduce the serum TNF-α level,and reduce the alveolar bone resorption in periodontitis rats.

关键词： psoralen alveolar bone resorption IL-10 TNF-α periodontitis

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Pure drug nano-assemblies: A facile carrier-free nanoplatform for efficient cancer therapy

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Acta Pharmaceutica Sinica B 2022年第1期12卷 92-106页

作者： Shuwen Fu Guanting Li Wenli Zang Xinyu Zhou Kexin Shi Yinglei Zhai School of Pharmacy Shenyang Pharmaceutical UniversityShenyang 110016China department of Pharmaceutics Wuya College of InnovationShenyang Pharmaceutical UniversityShenyang 110016China department of periodontology School and Hospital of StomatologyChina Medical UniversityLiaoning Provincial Key Laboratory of Oral DiseaseShenyang 110016China Bio-system Pharmacology Graduate School of MedicineFaculty of MedicineOsaka UniversityOsaka 565-0871Japan department of Biomedical Engineering School of Medical DeviceShenyang Pharmaceutical UniversityShenyang 110016China

Nanoparticulate drug delivery systems(Nano-DDSs) have emerged as possible solution to the obstacles of anticancer drug delivery. However, the clinical outcomes and translation are restricted by several drawbacks, such as low drug loading, premature drug leakage and carrier-related ***, pure drug nano-assemblies(PDNAs), fabricated by the self-assembly or co-assembly of pure drug molecules, have attracted considerable attention. Their facile and reproducible preparation technique helps to remove the bottleneck of nanomedicines including quality control, scale-up production and clinical translation. Acting as both carriers and cargos, the carrier-free PDNAs have an ultra-high or even100% drug loading. In addition, combination therapies based on PDNAs could possibly address the most intractable problems in cancer treatment, such as tumor metastasis and drug resistance. In the present review, the latest development of PDNAs for cancer treatment is overviewed. First, PDNAs are classified according to the composition of drug molecules, and the assembly mechanisms are discussed. Furthermore, the co-delivery of PDNAs for combination therapies is summarized, with special focus on the improvement of therapeutic outcomes. Finally, future prospects and challenges of PDNAs for efficient cancer therapy are spotlighted.

关键词： Nanotechnology Carrier-free Self-assembly Combination therapy Cancer treatment Pure drug Nanomedicine

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A 3D-printed molybdenum-containing scaffold exerts dual pro-osteogenic and anti-osteoclastogenic effects to facilitate alveolar bone repair

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International Journal of Oral Science 2022年第4期14卷 523-540页

作者： Beimin Tian Xuan Li Jiujiu Zhang Meng Zhang Dian Gan Daokun Deng Lijuan Sun Xiaotao He Chengtie Wu Faming Chen department of periodontology State Key Laboratory of Military Stomatology and National Clinical Research Center for Oral DiseasesShaanxi Engineering Research Center for Dental Materials and Advanced ManufactureSchool of StomatologyFourth Military Medical UniversityXi'anChina State Key Laboratory of High-Performance Ceramics and Superfine Microstructure Shanghai Institute of CeramicsChinese Academy of SciencesShanghaiChina

The positive regulation of bone-forming osteoblast activity and the negative feedback regulation of osteoclastic activity are equally important in strategies to achieve successful alveolar bone regeneration. Here, a molybdenum(Mo)-containing bioactive glass ceramic scaffold with solid-strut-packed structures(Mo-scaffold) was printed, and its ability to regulate pro-osteogenic and antiosteoclastogenic cellular responses was evaluated in vitro and in vivo. We found that extracts derived from Mo-scaffold(Moextracts) strongly stimulated osteogenic differentiation of bone marrow mesenchymal stem cells and inhibited differentiation of osteoclast progenitors. The identified comodulatory effect was further demonstrated to arise from Mo ions in the Mo-extract,wherein Mo ions suppressed osteoclastic differentiation by scavenging reactive oxygen species(ROS) and inhibiting mitochondrial biogenesis in osteoclasts. Consistent with the in vitro findings, the Mo-scaffold was found to significantly promote osteoblastmediated bone formation and inhibit osteoclast-mediated bone resorption throughout the bone healing process, leading to enhanced bone regeneration. In combination with our previous finding that Mo ions participate in material-mediated immunomodulation, this study offers the new insight that Mo ions facilitate bone repair by comodulating the balance between bone formation and resorption. Our findings suggest that Mo ions are multifunctional cellular modulators that can potentially be used in biomaterial design and bone tissue engineering.

关键词： osteoclast inhibited healing

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Impact of osteoporosis in dental implants:A systematic review

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World Journal of Orthopedics 2015年第2期6卷 311-315页

作者： Gabriela Giro Leandro Chambrone Abrao Goldstein Jose Augusto Rodrigues Elton Zenóbio Magda Feres Luciene Cristina Figueiredo Alessandra Cassoni Jamil Awad Shibli department of periodontology and Oral Implantology Dental Research DivisionUniversity of Guarulhos

AIM: To assess the failure and bone-to-implant contact rate of dental implants placed on osteoporotic subjects. METHODS: Extensive examination strategies were created to classify studies for this systematic review. MEDLINE(via Pub Med) and EMBASE database were examined for studies in English up to and including May 2014. The examination presented a combination of the MeS H words described as follow: "osteoporosis" or "osteopenia" or "estrogen deficiency" AND "implant" or "dental implant" or "osseointegration". Assessment of clinical and/or histological peri-implant conditions in osteoporosis subjects treated with titanium dental implants. The examination included a combination of the MeS H terms described as follow: "osteoporosis" or "osteopenia" or "estrogen deficiency" AND "implant" or "dental implant" or "osseointegration".RESULTS: Of 943 potentially eligible articles, 12 were included in the study. A total of 133 subjects with osteoporosis, 73 subjects diagnosed with osteopenia and 708 healthy subjects were assessed in this systematic review. In these subjects were installed 367, 205, 2981 dental implants in osteoporotic, osteopenic and healthy subjects, respectively. The failure rate of dental implant was 10.9% in osteoporotic subjects, 8.29% in osteopenic and 11.43% in healthy ones. Bone-to-implant contact obtained from retrieved implants ranged between 49.96% to 47.84%, for osteoporosis and non-osteoporotic subjects. CONCLUSION: Osteoporotic subjects presented higher rates of implant loss, however, there is a lower evidence to strengthen or refute the hypothesis that osteoporosis may have detrimental effects on bone healing. Consequently, final conclusions regarding the effect of osteoporosis in dental implant therapy cannot be made at this time. There are no randomized clinical trial accessible for evaluation and the retrospectivenature of the evaluated studies shall be taken in account when interpreting this study.

关键词： Dental implants Osteoporosis Failures Osteopenia Osseointegration

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MiR-4262 regulates differentiation and osteogenesis of humanperiodontal stem cells by targeting suppressor of cytokinesignaling 4

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BIOCELL 2020年第4期44卷 631-638页

作者： ZIMING WEI YARONG WANG MINGYUAN DU MINJIE LI HONG HE The State Key Laboratory Breeding Base of Basic Science of Stomatology(Hubei-MOST)&Key Laboratory of Oral Biomedicine Ministry of Education School&Hospital of StomatologyWuhan UniversityWuhan430079China department of Orthodontics Stomatological HospitalSouthern Medical UniversityGuangzhou510280China department of periodontology and Implantology Stomatological HospitalSouthern Medical UniversityGuangzhou510280China department of Orthodontics School and Hospital of StomatologyWuhan UniversityWuhan430079China

Periodontitis,as a chronic inflammatory disease,remains unsolved,and the pathogenesis of this disease has not been fully *** this study,the effect of miR-4262 was investigated in tumor necrosis factor-α(TNF-α)induced human periodontal stem cells(hPDLSCs)for the first *** gene expression involved in this study was determined using polymerase chain reaction(PCR),the expressions of relevant proteins were determined by western blot analysis,and the levels of IL-1β,IL-6,and MCP-1 were estimated by enzyme-linked immunosorbent assay(ELISA)*** luciferase reporter assay was performed for verification of the target gene,alkaline phosphatase(ALP)activity detection was used for differentiation capacity,and alizarin red staining assay was used for mineralization *** inhibition of miRNA-4262,which resulted in the upregulation of suppressor of cytokine signaling 4(SOCS4),showed protective effects,including anti-inflammation and promotional effects on osteogenesis as well as differentiation in TNF-αinduced *** results provided insights into the roles of miRNAs in regulating the inflammatory response,differentiation,and osteogenesis in hPDLSCs,which may promote the understanding of the mechanisms of periodontitis and find out a better therapeutic application.

关键词： TNF-α Periodontitis Differentiation Osteogenesis

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Allogenic tooth transplantation using 3D printing: A case report and review of the literature

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World Journal of Clinical Cases 2019年第17期7卷 2587-2596页

作者： Hu-Di Xu Richard J Miron Xiao-Xin Zhang Yu-Feng Zhang The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei- MOST) and Key Laboratory of Oral Biomedicine Ministry of Education Wuhan UniversityWuhan 430079Hubei ProvinceChina department of periodontology College of Dental MedicineNova Southeastern UniversityFort LauderdaleFL 33314-7796United States department of Oral Implantology School of StomatologyWuhan UniversityWuhan 430079Hubei ProvinceChina

BACKGROUND The history of allogenic tooth transplantation can be traced back to the 16th *** there have been many successful cases,much needs to be better understood and researched prior to the technique being translated to everyday clinical *** SUMMARY In the present report,we describe a case of allogenic tooth transplantation between a mother and her *** first left maxillary molar of the mother was diagnosed with residual root resorption and needed to be *** 3rd molar of the daughter was used as a donor *** to transplantation,a 3D printing system was introduced to fabricate an individualized reamer drill specifically designed utilizing the donor’s tooth as a *** specific design of our 3D printed bur allowed for the recipient site to better match the donor *** the ability to 3D print in layers,even the protuberance of the root can be matched and 3D printed,thereby minimizing unnecessary bone *** Our study is a pioneering case combining 3D printing with allogenic tooth transplantation,which could be able to minimize unnecessary bone loss and improve the implant *** article aims to enhance our understanding of allogenic tooth transplantation and 3D printing,and may potentially lead to tooth transplantation being utilized more frequently - especially since transplantations are so commonly utilized in many other fields of medicine with high success rates.

关键词： Transplantation Allografts Dental implants 3D printing Case report

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Dental stem cell-derived extracellular vesicles transfer miR-330-5p to treat traumatic brain injury by regulating microglia polarization

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International Journal of Oral Science 2022年第4期14卷 461-469页

作者： Ye Li Meng Sun Xinxin Wang Xiaoyu Cao Na Li Dandan Pei Ang Li Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research College of StomatologyXian Jiaotong UniversityXianChina State Key Laboratory Breeding Base of Basic Science of Stomatology(Hubei-MOST)&Key Laboratory of Oral Biomedicine Ministry of Education School and Hospital of StomatologyWuhan UniversityWuhanChina department of periodontology College of StomatologyXi'an Jiaotong UniversityXi'anChina

Traumatic brain injury(TBI)contributes to the key causative elements of neurological ***,no effective therapeutics have been developed *** our previous work,extracellular vesicles(EVs)secreted by stem cells from human exfoliated deciduous teeth(SHED)offered new insights as potential strategies for functional recovery of *** current study aims to elucidate the mechanism of action,providing novel therapeutic targets for future clinical *** the mi RNA array performed and Real-time PCR validated,we revealed the crucial function of mi R-330-5p transferred by SHED-derived EVs(SHED-EVs)in regulating microglia,the critical immune modulator in central nervous *** R-330-5p targeted Ehmt2 and mediated the transcription of CXCL14 to promote M2 microglia polarization and inhibit M1 *** in our in vivo data,SHED-EVs and their effector mi R-330-5p alleviated the secretion of inflammatory cytokines and resumed the motor functional recovery of TBI *** summary,by transferring mi R-330-5p,SHED-EVs favored anti-inflammatory microglia polarization through Ehmt2 mediated CXCL14transcription in treating traumatic brain injury.

关键词： injury transferred resume

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