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Chondroitinase ABC combined with Schwann cell transplantation enhances restoration of neural connection and functional recovery following acute and chronic spinal cord injury

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Neural Regeneration Research 2025年第5期20卷 1467-1482页

作者： Wenrui Qu Xiangbing Wu Wei Wu Ying Wang Yan Sun Lingxiao Deng Melissa Walker Chen Chen Heqiao Dai Qi Han Ying Ding Yongzhi Xia George Smith Rui Li Nai-Kui Liu Xiao-Ming Xu Spinal Cord and Brain Injury Research Group Stark Neurosciences Research InstituteDepartment of Neurological SurgeryIndiana University School of MedicineIndianapolisINUSA department of Hand surgery the Second Hospital of Jilin UniversityChangchunJilin ProvinceChina Shriners Hospitals pediatric Research Center Temple University School of MedicinePhiladelphiaPAUSA department of Anatomy&Cell Biology Indiana University School of MedicineIndianapolisINUSA

Schwann cell transplantation is considered one of the most promising cell-based therapy to repair injured spinal cord due to its unique growth-promoting and myelin-forming properties.A the Food and Drug Administration-approved Phase I clinical trial has been conducted to evaluate the safety of transplanted human autologous Schwann cells to treat patients with spinal cord injury.A major challenge for Schwann cell transplantation is that grafted Schwann cells are confined within the lesion cavity,and they do not migrate into the host environment due to the inhibitory barrier formed by injury-induced glial scar,thus limiting axonal reentry into the host spinal *** we introduce a combinatorial strategy by suppressing the inhibitory extracellular environment with injection of lentivirus-mediated transfection of chondroitinase ABC gene at the rostral and caudal borders of the lesion site and simultaneously leveraging the repair capacity of transplanted Schwann cells in adult rats following a mid-thoracic contusive spinal cord *** report that when the glial scar was degraded by chondroitinase ABC at the rostral and caudal lesion borders,Schwann cells migrated for considerable distances in both rostral and caudal *** Schwann cell migration led to enhanced axonal regrowth,including the serotonergic and dopaminergic axons originating from supraspinal regions,and promoted recovery of locomotor and urinary bladder ***,the Schwann cell survival and axonal regrowth persisted up to 6 months after the injury,even when treatment was delayed for 3 months to mimic chronic spinal cord *** findings collectively show promising evidence for a combinatorial strategy with chondroitinase ABC and Schwann cells in promoting remodeling and recovery of function following spinal cord injury.

关键词： axonal regrowth bladder function chondroitinase ABC functional recovery glial scar lentivirus migration Schwann cell spinal cord injury transplantation

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Diagnosis and surgical treatment of ruptured aneurysm in sinus of Valsalva

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Chinese Medical Journal 2003年第7期116卷 1047-1050页

作者：赵高峰僧靖静阎保君卫洪超乔晨晖赵松赵文增郅兴义 department of cardiovascular thoracic surgery the First Teaching Hospital Zhengzhou University department

Objective To evaluate the methods used to diagnose and surgically treat ruptured aneurysm in sinus of Valsalva (RASV) Methods Thirty seven hospitalized patients with ruptured aneurysms in the sinus of Valsalva from September 1981 to April 2001, including 21 cases (56 7%) of RASV associated with ventricular septal defects (VSD) and 11 (29 7%) with aortic valvular prolapse were given surgical interventions Under hypothermia and extracorporeal circulation, we successfully performed the surgical correction of RASV for all 37 patients VSD repair in 21 patients, aortic valvuloplasty in 6 and aortic valvular replacement in 2 Results There was no hospital deaths among these patients, although residual shunting occurred in two patients and acute renal failure was found one Follow up study of one month to 20 years in the patients undergoing repair of RASV revealed that the mostly individuals treated with operation obtained satisfactory cardiac function Conclusion Correct diagnosis of ruptured aneurysm in sinus of Valsalva should be confirmed immediately and surgical correction should be carried out as soon as possible

关键词： diagnosis aneurysm sinus of Vlsalva surgical treatment

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A Rare Case of Infective Mediastinitis after Melody Valve Implantation

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Congenital Heart Disease 2022年第2期17卷 187-192页

作者： Veronica Lorenz Karlien Carbonez Geoffroy de Beco Alain Poncelet department of cardiovascular and thoracic surgery Cliniques Universitaires Saint-LucBrusselsBelgium department of pediatric Cardiology Cliniques Universitaires Saint-LucBrusselsBelgium

Pulmonary valve implant is frequently necessary in children and adults with congenital heart *** endocarditis represents a rare but life-threatening complication after transcatheter pulmonary valve *** are various treatments for native or prosthetic valve *** intervention,combined with intravenous antibiotic treatment,is of paramount importance,in case of concomitant mediastinal infection,in order to ensure the radical debridement of all infected tissue,avoiding any recurrent *** this report,we describe a rare case of mediastinitis,associated with an infected endocarditis,occurring 8 months after Melody(Medtronic,Minneapolis,USA)valve implant,successfully treated with the implantation of a homograft to reconstruct the right ventricular outflow tract.

关键词： Transcatheter valve prosthesis infective endocarditis cardiac surgery congenital heart disease homograft

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Down-regulation of eIF5A-2 prevents epithelial-mesenchymal transition in non-small-cell lung cancer cells

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第6期14卷 460-467页

作者： Guo-dong XU Xin-bao SHI Le-bo SUN Qing-yun ZHOU Da-wei ZHENG Huo-shun SHI Yong-liang CHE Zi-shan WANG Guo-feng SHAO department of thoracic & cardiovascular surgery Lihuili HospitalNingbo Medical CenterAffiliated Hospital of Medical SchoolNingbo University department of thoracic & cardiovascular surgery Ningbo First HospitalAffiliated Hospital of Medical SchoolNingbo University

Background:Epithelial-mesenchymal transition(EMT) is believed to be the critical process in malignant tumor invasion and metastases,and has a great influence on improving the survival rate in non-small-cell lung cancer(NSCLC) *** studies suggested that eukaryotic initiation factor 5A-2(eIF5A-2) might serve as an adverse prognostic marker of *** detected eIF5A-2 in NSCLC A549 cells,and found that the invasive capability correlates with the eIF5A-2 ***:Transforming growth factor(TGF)-β1 was used to induce EMT in A549 *** blotting,immunofluorescence,wound healing assay,and transwell-matrigel invasion chambers were used to identify phenotype *** blotting was also used to observe changes of the expression of *** down-regulated the eIF5A-2 expression using an eIF5A-2 siRNA and identified the phenotype changes by western blotting and *** tested the change of migration and invasion capabilities of A549 cells by the wound healing assay and transwell-matrigel invasion ***:After stimulating with TGF-β1,almost all A549 cells changed to the mesenchymal phenotype and acquired more migration and invasion *** cells also had higher eIF5A-2 protein ***-regulation of eIF5A-2 expression with eIF5A-2 siRNA transfection could change the cells from mesenchymal to epithelial phenotype and decrease tumor cell migration and invasive capabilities ***:The expression of eIF5A-2 was up-regulated following EMT phenotype changes in A549 cells,which correlated with enhanced tumor invasion and metastatic ***,in the A549 cell line,the process of EMT phenotype change could be reversed by eIF5A-2 siRNA,with a consequent weakening of both invasive and metastatic capabilities.

关键词： Non-small-cell lung cancer(NSCLC) Epithelial-mesenchymal transition(EMT) Eukaryotic initiation factor 5A-2(eIF5A-2) Transforming growth factor(TGF)-β1 A549

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Effect and molecular mechanism of mir-146a on proliferation of lung cancer cells by targeting and regulating MIF gene

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Asian Pacific Journal of Tropical Medicine 2016年第8期9卷 783-788页

作者： Wu-Ming Wang Ji-Chun Liu Medical College of Nanchang University department of thoracic surgery Jiangxi Chest Hospital department of thoracic & cardiovascular surgery the First Affiliated Hospital of Nanchang University

Objective: To discuss the effect and molecular mechanism of mi R-146 a on the proliferation of lung cancer cells by targeting and regulating the macrophage migration inhibitory factor(MIF) gene. Methods: RT-PCR was employed to detect expression of mi R-146a; immunohistochemistry was used to detect the expression of MIF. The luciferase reporter gene technique was adopted to verify that MIF was the specific reverse target gene of mi R-146 a and the liposome LipofectamineTM2000 was employed to transfer the modeled mi R-146 a mimics, and mi R-146 a negative control(NC) in NSCLC cells to detect the expression of MIF m RNA and protein. MTT assay was used to detect cell viability, cloning technique to detect cell proliferation ability, Annexin V-PI to detect cell apoptosis, UV spectrophotometry to detect viability of cysteinyl aspartate specific proteinase 3(Caspase 3), and western blot to detect expression of nuclear factor-κB(NF-κB) in cells. Results: The expression of mi R-146 a in NSCLC lung tissues was lower than that in the normal lung tissues besides the lung cancer; while the expression of mi R-146 a in NSCLC cells was lower than that in normal human embryonic lung tissues. It was chosen as the subsequent cell line for its appropriate expression in A549. The expression of MIF protein in NSCLC lung tissues was higher than that in the normal lung tissues besides the lung cancer. The luciferase reporter gene proved that MIF was the reverse target gene of mi R-146 a. The mi R-146 a mimics were transfected into A549 cells through the liposome. Compared with NC group, the expression of MIF protein and m RNA was significantly decreased(P<0.01), with the decrease in the cell viability(P<0.01), the decrease in the number of clones(P<0.01), cell apoptosis(P<0.01), the increase in the activity of Caspase 3(P<0.01), and decrease in the phosphorylation of NF-κB p65(P<0.01). Conclusions: mi R-146 a has low expression in NSCLC tissues and cell lines, while MIF has the over expres

关键词： MicroRNA-146a Macrophage migration inhibition factor Non-small cell lung cancer Proliferation

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The diagnostic value of tenascin-C in acute aortic syndrome

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Journal of Geriatric Cardiology 2024年第3期21卷 359-368页

作者： Ming MA Wei CHEN Hai-Long CAO Jun PAN Qing ZHOU Xin-Long TANG Dong-Jin WANG department of thoracic and cardiovascular surgery Nanjing Drum Tower Hospital Clinical College of Nanjing Medical UniversityNanjingChina department of thoracic and cardiovascular surgery Nanjing Drum Tower HospitalAffiliated Hospital of Medical SchoolNanjing UniversityNanjingChina department of thoracic surgery Kunshan Hospital of Traditional Chinese MedicineJiangsuChina

OBJECTIVES Misdiagnosis of acute aortic syndrome(AAS)significantly increases ***-C(TN-C)is an extracellular matrix glycoprotein related to cardiovascular *** elevation of TN-C in AAS and whether it can discriminate suddenonset of acute chest pain in Chinese remains *** We measured the plasma concentration of TN-C by ELISA in a cohort of 376 patients with chest or back *** to discriminate AAS from acute coronary syndrome(ACS)were compared and *** From October 2016 to September 2021,376 undiagnosed patients with chest or back pain were enrolled.166 of them were finally diagnosed as AAS,100 were ACS and 110 without cardiovascular diseases(NCV).TN-C was significantly elevated in AAS at 18.18 ng/mL(IQR:13.10–27.68)compared with 7.51 ng/mL(IQR:5.67–11.38)in ACS(P<0.001)and 3.68 ng/mL(IQR:2.50–5.29)in NCV(P<0.001).There was no significant difference in TN-C level among the subtypes of *** the 166 AAS patients,the peaked level of TN-C was at acute stage(P=0.012),then a slight of decrease was observed at subacute *** area under receiver operating characteristic curve for AAS patients versus NCV was 0.979(95%CI:0.964-0.994)for *** a cutoff level of 11.474 ng/mL,TN-C has a sensitivity of 76.0%,specificity of 85.5%,accuracy of 82.0%,positive predictive value(PPV)of 76.0%,negative predictive value(NPV)of 85.5%.Diagnostic performance of TN-C was superior to D-dimer and *** The concentration of serum TN-C in AAS patients was significantly higher than that in ACS patients and ***-C could be a new biomarker to distinguish AAS patients in the early stage after symptoms onset from other pain diseases.

关键词： acute cardiovascular elevated

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Surgical outcomes and strategy of hypertrophic obstructive cardiomyopathy

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2006年第2期7卷 148-153页

作者：朱亚彬 RAJAN S. KURIAN V.M. 刘志勇 department of thoracic and cardiovascular surgery Affiliated Su Bei Hospital Yangzhou University Yangzhou 225001 China department of thoracic and cardiovascular surgery Madras Medical Mission Moggapair East Chennai 600037 India department of thoracic and cardiovascular surgery Affiliated Zhong Da Hospital Southeast University Nanjing 210009 China

Objective： To evaluate the surgical clinical results of hypertrophic obstructive cardiomyopathy. Methods： We retrospectively collected data on 24 patients who underwent surgical management in the past ten years in two hospitals in China and Madras Medical Mission in India. Myomectomy was carried out on all patients. Among them 3 patients underwent mitral valve replacement; 2 patients underwent mitral valve repair （anterior mitral leaflet plication）; 2 patients underwent aortic valve replacement; 1 patient underwent aortic valve repair; 2 patients underwent aortic root replacement; 1 patient underwent Bentall＇s procedure and 1 patient underwent coronary artery bypass grafting because of a breached muscle bridge, Results： One patient died of post-operative heart failure. The mean follow-up time was 4.3 years, There was significant improvement in the symptomatic status. Sixteen patients were asymptomatic with good effort tolerance and only four patients had New York heart association （NYHA） Classes Ⅰ-Ⅱ due to associated valvular lesions, Conclusion： Our experience proved that symptomatic hypertrophic obstructive cardiomyopathy or non-symptomatic hypertrophic obstructive cardiomyopathy with combined heart disease is indication for surgery as surgical intervention could get better clinical results in this kind of patients compared with other non-surgical method because it beneficially reduces the systolic anterior motion （SAM） of the mitral valve leaflet, which could not be avoided by other non-surgical treatment.

关键词： Hypertrophic obstructive cardiomyopathy Systolic anterior motion Myomectomy

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Surgical stent-graft implantation by open procedure for type B aortic dissection without optimal landing zone

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The Journal of Biomedical Research 2019年第5期33卷 357-362页

作者： Wei Qin Reinard Iskandar Liangpeng Li Fuhua Huang Xin Chen department of thoracic and cardiovascular surgery Nanjing First Hospital Nanjing Medical University

thoracic endovascular aortic repair(TEVAR)has been considered as a first-choice treatment for type B aortic dissection(TBAD).However,some patients that is lack of optimal landing zones(<15 mm in dissected Z2,Z3 or the presence of a lusorian artery)still pose significant challenges for *** utilized a surgical stent-graft implantation in the descending aorta combined with supra-aortic vessels transposition through median sternotomy for these special TBAD *** short-and mid-term results showed that our procedure is a good and alternative therapy for such kind patients.

关键词： Type B aortic dissection open surgery

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Perpulmonary Device Closure of Patent Ductus Arteriosus with Minimum Diameter More Than 4 mm in Infants

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Congenital Heart Disease 2022年第4期17卷 437-445页

作者： Shibin Sun Geoffrey J.Changwe Zeeshan Farhaj Hongxin Li Yuekun Sun Zhongzheng Kong department of cardiovascular surgery Shandong Qianfoshan HospitalCheeloo College of MedicineShandong UniversityJinanChina department of cardiovascular and thoracic surgery The National Heart HospitalLusakaZambia

Background:Closure of large patent ductus arteriosus(PDA)in older children has been accomplished using surgical and percutaneous techniques with remarkable ***,outcomes amongst infants have been variable with several *** we describe a novel minimally invasive technique,a product of mini-thoracotomy and traditional percutaneous technique skills,accomplished exclusively under echocardiography ***:Symptomatic infants with a significant left-to-right shunt from PDA measuring more than 4 mm were *** symptoms were varying degrees of tachypnea,tachycardia,heart failure,failure to thrive,recurrent respiratory tract infections,or intensive care unit treatment for a longer *** a left parasternal mini-thoracotomy,two parallel purse-string sutures were placed on the pulmonary *** purse-string circle puncture,under exclusively transesophageal echocardiography guidance,a device secured to the safety-suture was implanted on the ascending aorta via pulmonary trunk using a specially designed *** safety-suture prevented device migration in case of *** basic demographics,PDA size,device size and type,intrapulmonary manipulation time,operation time,PDA parameters(length,diameter,type of duct),redeployment of the device,residual shunt,and retention of safety-suture were all recorded and *** follow-up was done with transthoracic echocardiography on the 2^(nd)postoperative day,1,3,6,and 12 months,and yearly ***:Fifty-two infants with a mean age of 8 months±2.8 months(Interquartile range=0)underwent Perpulmonary device closure of *** PDA occlusion was accomplished event-free in all *** mean PDA,mean device,and mean operation time were 5.6 mm±1.4 mm,7.9 mm±1.7 mm,and 61.2 min±12.9 min,*** immediate acceptable residual shunt was noted among 3 subjects and disappeared at a 1-month *** infants had retained safety-suture for added ***

关键词： Patent ductus arteriosus perpulmonary device closure transesophageal echocardiography infant minimally invasive surgery

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Stress-induced cardiomyopathy with electrocardiographic ST-segment elevation in a patient with pneumothorax

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World Journal of Emergency Medicine 2020年第4期11卷 260-262页

作者： Jun Woo Cho Chi Hoon Bae department of thoracic and cardiovascular surgery School of MedicineCatholic University of DaeguDaeguRepublic of Korea

Dear editor,In many cases,S T-segment changes on electrocardiogram(ECG)are suggestive of acute coronary syndrome,but there may be other causes,[12]such as stress induced ***,reports of such changes in patients with pneumothorax are very rare.[3-1]Here,we report a case of ST-segment elevation due to stress-induced cardiomyopathy in a patient with pneumothorax.

关键词： cardiomyopathy acute patient

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