检索结果-南通市图书馆

Lithium promotes recovery after spinal cord injury

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2022年第6期17卷 1324-1333页

作者： Ying-Jie Zhao Hao Qiao Dong-Fan Liu Jie Li Jia-Xi Li Su-E Chang Teng Lu Feng-Tao Li Dong Wang Hao-Peng Li Xi-Jing He Fang Wang Department of Orthopedics the Second Affiliated Hospital of Xi’an Jiaotong University School of MedicineXi’anShaanxi ProvinceChina Department of Orthopedics Xi’an International Medical Center HospitalXi’anShaanxi ProvinceChina

Lithium is associated with oxidative stress and apoptosis,but the mechanism by which lithium protects against spinal cord injury remains poorly understood.In this study,we found that intraperitoneal administration of lithium chloride(LiCl)in a rat model of spinal cord injury alleviated pathological spinal cord injury and inhibited expression of tumor necrosis factorα,interleukin-6,and interleukin 1β.Lithium inhibited pyroptosis and reduced inflammation by inhibiting Caspase-1 expression,reducing the oxidative stress response,and inhibiting activation of the Nod-like receptor protein 3 inflammasome.We also investigated the neuroprotective effects of lithium intervention on oxygen/glucose-deprived PC12 cells.We found that lithium reduced inflammation,oxidative damage,apoptosis,and necrosis and up-regulated nuclear factor E2-related factor 2(Nrf2)and heme oxygenase-1 in PC12 cells.All-trans retinoic acid,an Nrf2 inhibitor,reversed the effects of lithium.These results suggest that lithium exerts anti-inflammatory,anti-oxidant,and anti-pyroptotic effects through the Nrf2/heme oxygenase-1 pathway to promote recovery after spinal cord injury.This study was approved by the Animal Ethics Committee of Xi’an Jiaotong University(approval No.2018-2053)on October 23,2018.

关键词： anti-inflammatory anti-oxidant anti-pyroptosis cell death inflammatory factors lithium neurons neuroprotective Nrf2/HO-1 spinal cord injury

Internal fixator vs external fixator in the management of unstable pelvic ring injuries:A prospective comparative cohort study

在线全文

学校读者我要写书评

暂无评论

World Journal of orthopedics 2023年第7期14卷 562-571页

作者： Mohamed Abo-Elsoud Mostafa I Awad Mahmoud Abdel Karim Sherif Khaled Mohamed Abdelmoneim Department of Orthopedics and Traumatology Cairo University HospitalsCairo 11562Egypt department of Trauma and orthopedics Mataria Teaching HospitalCairo 4540046Egypt

BACKGROUND Reconstruction of the pelvic ring anatomy in unstable anterior pelvic ring injuries is a significant step to reduce the mortality rate associated with these injuries efficiently.There is a debate on using either an anterior subcutaneous pelvis internal fixator(INFIX)or an anterior supra-acetabular external fixator(EXFIX)to manage an unstable anterior pelvic ring fracture.AIM To compare the functional and radiological outcomes and complications of INFIX vs EXFIX in managing unstable pelvic ring injuries.METHODS A prospective cohort study included 54 patients with unstable pelvic ring fractures.The patients were divided into two groups;the INFIX group,in which 30 cases were fixed by INFIX,and the EXFIX group,in which 24 patients were treated by EXFIX.The average age in the EXFIX group was 31.17 years(16-57 years),while in the INFIX group,it was 34.5 years(17-53 years).The study included 20(66.7%)males and 10(33.3%)females in the INFIX group and 10(41.7%)males and 14(58.3%)females in the EXFIX group.The radiological outcomes were evaluated using Matta and Tornetta's score,and the functional outcomes using the Majeed score.RESULTS The results revealed a statistically significant difference between both groups(P=0.013)regarding radiological outcomes,according to Matta and Tornetta's score in favor of the INFIX group.Sitting,standing,and walking abilities were measured at a 3-mo follow-up visit using Majeed score modules.It was significantly better among the INFIX group than the EXFIX group in all three modules.At the final follow-up,both groups had no statistically significant difference according to the Majeed score;92.35 in the INFIX group and 90.99 in the EXFIX group(P=0.513).A lower surgical site infection rate was noticed in the INFIX group(P=0.007).CONCLUSION Anterior subcutaneous pelvis INFIX is associated with better radiological outcomes and a lower infection rate than anterior supra-acetabular EXFIX in managing patients with unstable anterior pelvic ring fractures.

关键词： Internal fixator External fixator Unstable Anterior Pelvic Injuries

Changes of serum inflammatory factors and miR-145 expression in patients with osteoarthritis before and after treatment and their clinical value

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Clinical Cases 2019年第19期7卷 2963-2975页

作者： Xiao-Zhen Wang Wen-Xue Li Department of Orthopedics Dongying Shengli Hospital

BACKGROUND Osteoarthritis is a chronic degenerative disease with an incidence of 50%in people over 65 years old and 80%in people over 80 years old worldwide.It is the second leading reason of loss of working capacity after cardiovascular diseases and severely affects the society and families.Therefore,finding biological markers related to the diagnosis and treatment of osteoarthritis is of great significance in clinical practice.AIM To observe the changes and clinical value of serum inflammatory factors and miR-145 expression in patients with osteoarthritis before and after treatment.METHODS Eighty-three patients with knee osteoarthritis(observation group)who were admitted to our hospital from April 2013 to June 2015,and 60 healthy people(control group)during the same period were selected.After 4 wk of treatment,the levels of miR-145,tumor necrosis factor(TNF)-α,interleukin(IL)-6,and IL-10 were compared between the control group and the observation group before treatment.The correlation of miR-145,TNF-α,IL-6,and IL-10 levels with visual analogue scale(VAS),Lysholm,and Western Ontario and McMaster Universities Osteoarthritis Index(WOMAC)scores was assessed by Pearson correlation analysis.The correlation of the expression of miR-145,TNF-α,IL-6,and IL-10 with Kellgren-Lawrence(K-L)grades was assessed by Spearman correlation analysis.The critical levels of miR-145,TNF-α,IL-6,and IL-10 in distinguishing different K-L grades were determined by receiver operating characteristic(ROC)curve analysis.RESULTS The expression level of miR-145 in the observation group was significantly higher than that in the control group before treatment(P<0.05).After treatment,the expression level of miR-145 in the observation group was significantly lower than that before treatment(P<0.05).The levels of TNF-αand IL-6 in the observation group were significantly higher than those in the control group(P<0.05),and the level of IL-10 was significantly lower than that in the control group(P<0.05).After treatment,the levels of TNF-αand IL-6 in the

关键词： Osteoarthritis Treatment Inflammatory factor miR-145

Establishment of a rat model of chronic thoracolumbar cord compression with a flat plastic screw

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2016年第6期11卷 963-970页

作者： Yong Sun Li-hai Zhang Yang-mu Fu Zhi-rui Li Jian-heng Liu Jiang Peng Bin Liu Pei-fu Tang Department of Orthopedics Chinese PLA General Hospital Department of Orthopedics Research Institute Chinese PLA General Hospital

Previous studies of animal models of chronic mechanical compression of the spinal cord have mainly focused on cervical and thoracic lesions, but few studies have investigated thoracolumbar injury. The specific pathophysiological mechanism of chronic thoracolumbar cord injury has not yet been elucidated. The purpose of this study was to improve animal models of chronic thoracolumbar cord compression using the progressive screw. A custom-designed flat plastic screw was implanted in the spinal cord between thoracic vertebrae 12 and lumbar 1 of rats. The screw was tightened one complete turn（0.5 mm） every 7 days for 4 weeks to create different levels of chronic spinal cord compression. Following insertion of the screw, there was a significant decline in motor function of the hind limbs, and severe stenosis of micro-computed tomography parameters in the spinal cord. Cortical somatosensory evoked potential amplitudes were reduced remarkably, and latencies were prolonged at 30 minutes after surgery. The loss of motor neurons in the gray matter was marked. Demyelination and cavitation were observed in the white matter. An appropriate rat model of chronic thoracolumbar cord compression was successfully created using the progressive screw compression method, which simulated spinal cord compression injury.

关键词： screw thoracic lumbar vertebral minutes evoked stenosis progressive custom prolonged

A morphological study of age-related changes in medullary characteristics of proximal humerus

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2022年第3期135卷 359-361页

作者： Wei Zhang Zuhao Chang Jiantao Li Peifu Tang Hua Chen Department of Orthopedics Chinese PLA General HospitalBeijing 100853China

To the Editor:Proximal humeral fracture(PHF)predominantly occurs in patients older than 60 years old with severe osteoporosis.[1]Although a majority of PHFs can be treated non-operatively,the complicated ones require surgical fixation.[2]In older individuals,the loss of trabecular structure and the calcar region in the proximal humeral medullary cavity makes them prone to comminuted fracture and loss of medial hinge support,leading to a high rate of implant failure and reoperation.[3]Recent studies have shown that endosteal augments incorporated into the locking plate construct might provide better medullary support and mechanical stability.[1]However,the endosteal augments used in PHF fixation are of different shapes,modes,and positions,which can lead to implant failure and reduction loss.[1,4,5]This may be because of a lack of understanding of the morphological changes that take place in the proximal humerus at different ages.Therefore,in this study,we analyzed and compared the anatomical degeneration pattern of the medullary canal between older and younger patients to have a better understanding of the medullary morphology of proximal humerus anatomically and the application of endosteal support clinically.

关键词： stability. fracture younger

Superenhancers activate the autophagy-related genes Beclin1 and LC3B to drive metastasis and drug resistance in osteosarcoma

在线全文

学校读者我要写书评

暂无评论

Frontiers of Medicine 2022年第6期16卷 883-895页

作者： Hongyi Wang Zhuochao Liu Jun Wang Fangqiong Hu Qi Zhou Li Wei Qiyuan Bao Jizhuang Wang Jing Liang Zhihong Liu Weibin Zhang Department of Orthopedics Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai200025China Shanghai Institute of Traumatology and orthopedics Shanghai200025China

Metastasis and drug resistance are the leading causes of poor prognosis in patients with osteosarcoma.Identifying the relevant factors that drive metastasis and drug resistance is the key to improving the therapeutic outcome of osteosarcoma.Here,we reported that autophagy was highly activated in metastatic osteosarcoma.We found increased autophagolysosomes in metastatic osteosarcoma cell lines by using electron microscopy,Western blot,and immunofluorescence experiments.We further examined the expression of the autophagy-related genes Beclin1 and LC3B in 82 patients through immunohistochemistry and found that Beclin1 and LC3B were highly related to unfavorable prognosis of osteosarcoma.Knockdown of Beclin1 and LC3B reduced invasion,metastasis,and proliferation in metastatic osteosarcoma cells.In vitro and in vivo studies also demonstrated that inhibiting by 3-MA inhibited cell growth and metastasis.Moreover,we demonstrated that autophagy-related genes were activated by SEs and that the inhibition of SEs by JQ-1 decreased the metastasis of osteosarcoma.Overall,our findings highlighted the association of autophagy with osteosarcoma progression and shed new light on autophagy-targeting therapy for osteosarcoma.

关键词： osteosarcoma autophagy metastasis drug resistance Beclin1 LC3B

Oscillating field stimulation promotes neurogenesis of neural stem cells through miR-124/Tal1 axis to repair spinal cord injury in rats

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2023年第4期18卷 895-900页

作者： Chao Fang Jian Sun Jun Qian Cai-Liang Shen Department of Orthopedics&Spine Surgery The First Affiliated Hospital of Anhui Medical UniversityHefeiAnhui ProvinceChina Department of Orthopedics The Second Affiliated Hospital of Anhui Medical UniversityHefeiAnhui ProvinceChina

Spinal cord injury often leads to severe motor and sensory deficits,and prognosis using the currently available therapies remains poor.Therefore,we aimed to explore a novel therapeutic approach for improving the prognosis of spinal cord injury.In this study,we implanted oscillating field stimulation devices and transplanted neural stem cells into the thoracic region(T9–T10)of rats with a spinal cord contusion.Basso-Beattie-Bresnahan scoring revealed that oscillating field stimulation combined with neural stem cells transplantation promoted motor function recovery following spinal cord injury.In addition,we investigated the regulation of oscillating field stimulation on the miR-124/Tal1 axis in neural stem cells.Transfection of lentivirus was performed to investigate the role of Tal1 in neurogenesis of neural stem cells induced by oscillating field stimulation.Quantitative reverse transcription-polymerase chain reaction,immunofluorescence and western blotting showed that oscillating field stimulation promoted neurogenesis of neural stem cells in vitro and in vivo.Hematoxylin and eosin staining showed that oscillating field stimulation combined with neural stem cells transplantation alleviated cavities formation after spinal cord injury.Taking the results together,we concluded that oscillating field stimulation decreased miR-124 expression and increased Tal1 content,thereby promoting the neurogenesis of neural stem cells.The combination of oscillating field stimulation and neural stem cells transplantation improved neurogenesis,and thereby promoted structural and functional recovery after spinal cord injury.

关键词： miR-124 neural stem cell neurogenesis oscillating field stimulation recovery spinal cord injury Tal1 tissue repair transplantation

Medial trochlea superior surface—a new bony landmark to assist the femoral rotational alignment in total knee arthroplasty

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2022年第22期135卷 2761-2763页

作者： Yuan Liu Jun Ma Limin Wu Xianchao Bao Jing Yang Bin Shen orthopedics Research Institute Department of OrthopedicsNational Clinical Research Center for GeriatricsWest China HospitalSichuan UniversityChengduSichuan 610041China

To the Editor:Femoral rotational alignment not only affects patellar tracking and alters peak contact forces,but also determines the flexion gap of the components.[1]If the angle of the external rotation is too large,it would lead to medial compartment loosing during knee flexion;if the angle is too small,it would lead to a narrowing medial compartment.A series of studies have found that the malalignment of femoral prosthesis resulted in poor outcomes after total knee arthroplasty(TKA),such as the lower Knee Society Scores,flexion instability,premature wear of the polyethylene,premature loosening of component and ongoing knee pain.It is helpful to introduce a new bony landmark to assist the femoral rotational alignment,and decrease the adverse effects caused by poor alignment of femoral rotation in TKA.The surface upon the medial trochlea,which was named as medial trochlea superior surface(MTSS)in this research,was found possibly parallel to the surgical trans-epicondylar axis(sTEA),which was usually used as a referential axis for femoral rotational alignment in TKA.Hence,MTSS might be used as a referential surface in TKA for its special anatomical position.The medial boundary of MTSS is close to the axis of the femoral shaft,and the distal end is immediately adjacent to the upper edge of the medial trochlea.The specific location of MTSS in TKA is shown in Figure 1A.

关键词： femoral medial knee

Inhibiting MMP13 Attenuates Deep Vein Thrombosis in a Mouse Model by Reducing the Expression of Pdpn

在线全文

学校读者我要写书评

暂无评论

当代医学科学（英文） 2024年第2期44卷 369-379页

作者： Ji LUO Jin ZHOU Jing-zeng LUO Hai-long WANG Xue-ling ZHAO Ru-dan ZHOU department of Intensive Care Unit The First People's Hospital of ZiyangZiyang 641300China Department of Orthopedics Kunming Medical University Second Affiliated HospitalKunming 650033China Department of Orthopedics Kunming Medical University First Affiliated HospitalKunming 650032China Department of Orthopedics The Third People's Hospital of Yunnan ProvinceKunming 650200China

Objective:Matrix metalloproteinase 13(MMP13)is an extracellular matrix protease that affects the progression of atherosclerotic plaques and arterial thrombi by degrading collagens,modifying protein structures and regulating inflammatory responses,but its role in deep vein thrombosis(DVT)has not been determined.The purpose of this study was to investigate the potential effects of MMP13 and MMP13-related genes on the formation of DVT.Methods:We altered the expression level of MMP13 in vivo and conducted a transcriptome study to examine the expression and relationship between MMP13 and MMP13-related genes in a mouse model of DVT.After screening genes possibly related to MMP13 in DVT mice,the expression levels of candidate genes in human umbilical vein endothelial cells(HUVECs)and the venous wall were evaluated.The effect of MMP13 on platelet aggregation in HUVECs was investigated in vitro.Results:Among the differentially expressed genes,interleukin 1 beta,podoplanin(Pdpn),and factor Ⅷvon Willebrand factor(F8VWF)were selected for analysis in mice.When MMP13 was inhibited,the expression level of PDPN decreased significantly in vitro.In HUVECs,overexpression of MMP13 led to an increase in the expression level of PDPN and induced platelet aggregation,while transfection of PDPN-siRNA weakened the ability of MMP13 to increase platelet aggregation.Conclusions:Inhibiting the expression of MMP13 could reduce the burden of DVT in mice.The mechanism involves downregulating the expression of Pdpn through MMP13,which could provide a novel gene target for DVT diagnosis and treatment.

关键词： deep vein thrombosis matrix metalloproteinase 13 podoplanin