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Polyphyllin Ⅰ enhances tumor necrosis factor-related apoptosis-inducing ligand-induced inhibition of human osteosarcoma cell growth via downregulating the Wnt/β-catenin pathway

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Journal of Traditional Chinese Medicine 2024年第2期44卷 251-259页

作者： CHANG Junli ZHAO Fulai SUN Xingyuan MA Xiaoping ZHAO Peng ZHOU Chujie SHI Binhao GU Wenchao WANG Yongjun YANG Yanping spine Disease Institute Longhua Hospital Affiliated to Shanghai University of Traditional Chinese MedicineShanghai 200032China department of Diagnostic and Interventional Radiology University of TsukubaIbaraki 315-0114Japan

OBJECTIVE:To investigate the synergistic effects of polyphyllin Ⅰ(PPⅠ)combined with tumor necrosis factorrelated apoptosis-inducing ligand(TRAIL)on the growth of osteosarcoma cells through downregulating the Wnt/β-catenin signaling ***:Cell viability,apoptosis and cell cycle distribution were examined using cell counting kit-8 and flow cytometry *** morphology of cancer cells was observed with inverted phase contrast *** migration and invasion abilities were examined by xCELLigence real time cell analysis DP system and transwell *** expressions of poly(adenosine diphosphate-ribose)polymerase,C-Myc,Cyclin B1,cyclin-dependent kinases 1,N-cadherin,Vimentin,Active-β-catenin,β-catenin,p-glycogen synthase kinase 3β(GSK-3β)and GSK-3βwere determined by Western blotting ***:PPⅠ sensitized TRAIL-induced decrease of viability,migration and invasion,as well as increase of apoptosis and cell cycle arrest of MG-63 and U-2 OS osteosarcoma *** synergistic effect of PPⅠwith TRAIL in inhibiting the growth of osteosarcoma cells was at least partially realized through the inactivation of Wnt/β-catenin signaling ***:The combination of PPⅠ and TRAIL is potentially a novel treatment strategy of osteosarcoma.

关键词： osteosarcoma tumor necrosis factor-related apoptosis-inducing ligand Wnt signaling pathway beta-catenin polyphyllinⅠ

Icariin accelerates bone regeneration by inducing osteogenesisangiogenesis coupling in rats with type 1 diabetes mellitus

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World Journal of Diabetes 2024年第4期15卷 769-782页

作者： Sheng Zheng Guan-Yu Hu Jun-Hua Li Jia Zheng Yi-Kai Li department of Traditional Chinese orthopedics and Traumatology Center for Orthopedic SurgeryThe Third Affiliated Hospital of Southern Medical UniversityGuangzhou 510630Guangdong ProvinceChina School of Traditional Chinese Medicine Southern Medical UniversityGuangzhou 510515Guangdong ProvinceChina department of Endocrinology Peking University First HospitalBeijing 100034China

BACKGROUND Icariin(ICA),a natural flavonoid compound monomer,has multiple pharmacological ***,its effect on bone defect in the context of type 1 diabetes mellitus(T1DM)has not yet been *** To explore the role and potential mechanism of ICA on bone defect in the context of *** The effects of ICA on osteogenesis and angiogenesis were evaluated by alkaline phosphatase staining,alizarin red S staining,quantitative real-time polymerase chain reaction,Western blot,and ***-related assays were conducted to investigate the relationship between osteogenesis and angiogenesis.A bone defect model was established in T1DM *** model rats were then treated with ICA or placebo and micron-scale computed tomography,histomorphometry,histology,and sequential fluorescent labeling were used to evaluate the effect of ICA on bone formation in the defect *** ICA promoted bone marrow mesenchymal stem cell(BMSC)proliferation and osteogenic *** ICA treated-BMSCs showed higher expression levels of osteogenesis-related markers(alkaline phosphatase and osteocalcin)and angiogenesis-related markers(vascular endothelial growth factor A and platelet endothelial cell adhesion molecule 1)compared to the untreated *** was also found to induce osteogenesis-angiogenesis coupling of *** the bone defect model T1DM rats,ICA facilitated bone formation and CD31hiEMCNhi type H-positive capillary ***,ICA effectively accelerated the rate of bone formation in the defect *** ICA was able to accelerate bone regeneration in a T1DM rat model by inducing osteogenesis-angiogenesis coupling of BMSCs.

关键词： Icariin Osteogenesis-angiogenesis coupling Type 1 diabetes mellitus Bone defect Bone regeneration

Skin and Soft Tissue Infections in the Surgical Area at the Kara Teaching Hospital

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Surgical Science 2024年第2期15卷 48-53页

作者： Tamegnon Dossouvi Tchaa Hodabalo Towoezim Efoé-Ga Olivier Amouzou Kokou Kouliwa Kanassoua Irokoura Kassagne Ayi Amavi Abossisso Sakiye Komlan Adabra Ekoué David Dosseh department of General surgery Dapaong Regional Hospital Center Dapaong Togo department of orthopedic and Traumatology Kara Teaching Hospital Kara Togo department of General surgery Kara Regional Hospital Center Kara Togo department of General surgery Sylvanus Olympio University Hospital Lomé Togo

Objective: To report the management of skin and soft tissue infections in the surgical area of Kara University Hospital in Togo. Material and Methods: This study was conducted retrospectively from January 1, 2021, to December 31, 2022, in the general surgery and orthopedic trauma departments. The study focused on soft tissue infections of the pelvic and thoracic limbs and analyzed epidemiological, clinical, paraclinical, therapeutic, and evolutionary data. Results: We registered 165 patients, comprising 109 men and 56 *** sex ratio (F/H) were 0.51. The mean age was 45 years with extremes ranging from 23 to 90 years. Farmers (64.8%) followed by housewives (34.0%) were the social strata most affected. The consultation period varied between 1 and 90 days. The pathologies found were necrotizing fasciitis (53.3%), erysipelas (18.2%), infected limb wounds (12.1%), pyomyositis (9.7%), and necrotizing dermo-hypodermitis (1.8%). The main procedures performed were necrosectomy and grafting (62.9%), sample necrosectomy (18.8%), drainage (9.7%), and pelvic limb amputation (1.2%). Follow-up was favorable in 86.7% of cases. The study noted a death rate of 13.3% due to septic shock secondary to a delay in consultation. Conclusion: Skin and soft tissue infections were a common reason for surgical hospitalization at Kara University Hospital, with a high mortality rate due to delayed consultations.

关键词： Skin and Soft Tissue Infections Necrotizing Fasciitis Erysipelas Emergency

Mid-term outcomes of a kinematically designed cruciate retaining total knee arthroplasty

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World Journal of orthopedics 2024年第2期15卷 118-128页

作者： Jonathan L Katzman Akram A Habibi Muhammad A Haider Casey Cardillo Ivan Fernandez-Madrid Morteza Meftah Ran Schwarzkopf department of orthopedic surgery Division of Adult Reconstructive SurgeryNYU Langone Orthopedic HospitalNYU Langone HealthNew YorkNY 10010United States

BACKGROUND Advances in implant material and design have allowed for improvements in total knee arthroplasty(TKA)outcomes.A cruciate retaining(CR)TKA provides the least constraint of TKA designs by preserving the native posterior cruciate *** research exists that has examined clinical outcomes or patient reported outcome measures(PROMs)of a large cohort of patients undergoing a CR TKA utilizing a kinematically designed *** was hypothesized that the studied CR Knee System would demonstrate favorable outcomes and a clinically significant improvement in pain and functional *** To assess both short-term and mid-term clinical outcomes and PROMs of a novel CR TKA *** A retrospective,multi-surgeon study identified 255 knees undergoing a TKA utilizing a kinematically designed CR Knee System(JOURNEY™II CR;Smith and Nephew,Inc.,Memphis,TN)at an urban,academic medical institution between March 2015 and July 2021 with a minimum of two-years of clinical follow-up with an orthopedic *** demographics,surgical information,clinical outcomes,and PROMs data were collected via query of electronic medical *** PROMs collected in the present study included the Knee Injury and Osteoarthritis Outcome Score for Joint Replacement(KOOS JR)and Patient-Reported Outcomes Measurement Information System(PROMIS■)*** significance of improvements in mean PROM scores from preoperative scores to scores collected at six months and two-years postoperatively was analyzed using Independent Samples *** Of the 255 patients,65.5%were female,43.8%were White,and patients had an average age of 60.6 *** osteoarthritis(96.9%)was the most common primary *** mean surgical time was 105.3 minutes and mean length of stay was 2.1 d with most patients discharged home(92.5%).There were 18 emergency department(ED)visits within 90 d of surgery resulting in a 90 d ED visit rate of 7.1%,including a 2.4%orthopedic-related ED visit ra

关键词： Total knee arthroplasty Cruciate retaining Kinematic design Survivorship Bearing material Prosthetic design Clinical outcomes Patient-reported outcome measures

6DOF pose estimation of a 3D rigid object based on edge-enhanced point pair features

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Computational Visual Media 2024年第1期10卷 61-77页

作者： Chenyi Liu Fei Chen Lu Deng Renjiao Yi Lintao Zheng Chenyang Zhu Jia Wang Kai Xu College of Computing National University of Defense TechnologyChangsha410073China department of spine surgery the Second Xiangya HospitalCentral South UniversityChangsha410011China Clinical Nursing Teaching and Research Section the Second Xiangya HospitalChangsha410011China College of Meteorology and Oceanography National University of Defense TechnologyChangsha410073China Beijing Institute of Tracking and Communication Technology Beijing100094China

The point pair feature(PPF)is widely used for 6D pose *** this paper,we propose an efficient 6D pose estimation method based on the PPF *** introduce a well-targeted down-sampling strategy that focuses on edge areas for efficient feature extraction for complex geometry.A pose hypothesis validation approach is proposed to resolve ambiguity due to symmetry by calculating the edge matching *** perform evaluations on two challenging datasets and one real-world collected dataset,demonstrating the superiority of our method for pose estimation for geometrically complex,occluded,symmetrical *** further validate our method by applying it to simulated punctures.

关键词： point pair feature(PPF) pose estimation object recognition 3D point cloud

Hypermethylation of the ALOX12 and CBS promoters in osteoporosis:Potential biomarkers for early diagnosis

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Genes & Diseases 2024年第1期11卷 30-33页

作者： Shang Guo Yue Wu Weekai Chia Peichun Hsu Hongwei Wang Yunliang Wang Biao Zhong department of orthopedic surgery Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200023China Institute of Translational Medicine Shanghai General HospitalShanghai Jiao Tong University School of Medicine(SJTU-SM)Shanghai 200025China The University of Chicago Department of MedicineChicagoIL 60637USA department of Neurology The Second Affiliated Hospital of Zhengzhou UniversityZhengzhouHenan 450014China

As a bone-confined chronic degenerative disorder,the progression of osteoporosis is characterized by abnormal crosstalk between osteoblasts and osteoclasts,leading to an imbalance of bone remodeling in adults.1 Several studies reported that arachidonic acid ester 12 lipoxygenase(ALOX12)acts as a regulator in bone genesis by participating in the activation of the peroxisome proliferatoractivated receptor(PPARG)pathway through its reaction product.

关键词： osteoporosis diagnosis remodeling

Microalgae-based drug delivery system for tumor microenvironment photo-modulating and synergistic chemo-photodynamic therapy of osteosarcoma

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Engineered Regeneration 2024年第2期5卷 199-209页

作者： Feng Liang Xueying An Ruoxi Wang Wenshu Wu Lin Yang Yixin Zheng Qing Jiang Xingquan Xu Danni Zhong Min Zhou Eye Center The Second Affiliated HospitalZhejiang University School of MedicineHangzhou310000PR China department of orthopedics The First Affiliated HospitalZhejiang University School of MedicineHangzhou310000PR China State Key Laboratory of Pharmaceutical Biotechnology Division of Sports Medicine and Adult Reconstructive SurgeryDepartment of Orthopedic SurgeryNanjing Drum Tower HospitalThe Affiliated Hospital of Nanjing University Medical SchoolNanjing210008PR China Zhejiang University-University of Edinburgh Institute(ZJU-UoE Institute) Zhejiang University School of MedicineZhejiang UniversityHaining314400PR China State Key Laboratory of Pharmaceutical Biotechnology Division of Sports Medicine and Adult Reconstructive SurgeryDepartment of Orthopedic SurgeryNanjing Drum Tower HospitalClinical College of Nanjing Medical UniversityNanjing210008PR China Institute of Translational Medicine Zhejiang UniversityHangzhou310029PR China Zhejiang University-Ordos City Etuoke Banner Joint Research Center Zhejiang UniversityHaining314400PR China

Osteosarcoma(OS)is one of the most common malignant tumors in children and young *** chemotherapy and other therapies are limited by low therapeutic efficiency,severe side effects and single therapeutic function,it is of high value to develop innovative therapies for precise and efficient treatment of ***,natural photo-synthetic microalgae(***,CV)were utilized as carriers for the chemotherapeutic agent doxorubicin(DOX)to create a multifunctional therapeutic platform(CV@DOX)for the photo-modulation of the tumor microenvi-ronment(TME)and synergistic chemo-photodynamic therapy of ***@DOX exhibited rapid drug release behavior in the acidic TME,improving the efficiency of chemotherapy against tumors and reducing side effects on other normal *** 650 nm laser irradiation,CV@DOX demonstrated the ability to effectively generate oxygen to alleviate tumor hypoxia and utilize the photosensitizing properties of chlorophyll in CV to produce an increased amount of reactive oxygen species(ROS),thereby enhancing photodynamic therapy(PDT).CV@DOX-mediated synergistic chemo-photodynamic therapy demonstrated efficacy in halting tumor progression in an orthotopic osteosarcoma mouse model by promoting tumor cell apoptosis,inhibiting tumor proliferation and ***,chlorophyll-assisted fluorescence imaging enabled monitoring of the distribution of CV@DOX in osteosarcoma after ***,CV@DOX did not cause significant hematological and tissue toxicity,and prevented DOX-induced cardiotoxicity,showing good in vivo ***,this work presents a novel TME-responsive and TME-modulating platform for imaging-guided multimodal osteosar-coma treatment.

关键词： Osteosarcoma Tumor hypoxia Photodynamic Chemotherapy Synergistic therapy

Additively manufactured Ti–Ta–Cu alloys for the next-generation load-bearing implants

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International Journal of Extreme Manufacturing 2024年第1期6卷 353-374页

作者： Amit Bandyopadhyay Indranath Mitra Sushant Ciliveri Jose D Avila William Dernell Stuart B Goodman Susmita Bose W.M.Keck Biomedical Materials Research Laboratory School of Mechanical and Materials EngineeringWashington State UniversityPullmanWA 99164United States of America Veterinary Clinical Sciences College of Veterinary MedicineWashington State UniversityPullmanWA 99164United States of America department of orthopedic surgery Stanford University Medical CenterRedwood CityCA 94063United States of America

Bacterial colonization of orthopedic implants is one of the leading causes of failure and clinical complexities for load-bearing metallic implants. Topical or systemic administration of antibiotics may not offer the most efficient defense against colonization, especially in the case of secondary infection, leading to surgical removal of implants and in some cases even limbs. In this study, laser powder bed fusion was implemented to fabricate Ti3Al2V alloy by a 1:1 weight mixture of CpTi and Ti6Al4V powders. Ti-Tantalum(Ta)–Copper(Cu) alloys were further analyzed by the addition of Ta and Cu into the Ti3Al2V custom alloy. The biological,mechanical, and tribo-biocorrosion properties of Ti3Al2V alloy were evaluated. A 10 wt.% Ta(10Ta) and 3 wt.% Cu(3Cu) were added to the Ti3Al2V alloy to enhance biocompatibility and impart inherent bacterial resistance. Additively manufactured implants were investigated for resistance against Pseudomonas aeruginosa and Staphylococcus aureus strains of bacteria for up to 48 h. A 3 wt.% Cu addition to Ti3Al2V displayed improved antibacterial efficacy, i.e.78%–86% with respect to CpTi. Mechanical properties for Ti3Al2V–10Ta–3Cu alloy were evaluated, demonstrating excellent fatigue resistance, exceptional shear strength, and improved tribological and tribo-biocorrosion characteristics when compared to Ti6Al4V. In vivo studies using a rat distal femur model revealed improved early-stage osseointegration for alloys with10 wt.% Ta addition compared to CpTi and Ti6Al4V. The 3 wt.% Cu-added compositions displayed biocompatibility and no adverse infammatory response in vivo. Our results establish the Ti3Al2V–10Ta–3Cu alloy’s synergistic effect on improving both in vivo biocompatibility and microbial resistance for the next generation of load-bearing metallic implants.

关键词： Ti6Al4V load-bearing implants additive manufacturing 3D printing antibacterial performance

BMSC-derived Exosomes Ameliorate Peritoneal Dialysis-associated Peritoneal Fibrosis via the Mir-27a-3p/TP53 Pathway

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Current Medical Science 2024年第2期44卷 333-345页

作者： Jun-li ZHAO Lin ZHAO Qiu-nan ZHAN Miao LIU Ting ZHANG Wen-wen CHU department of Nephrology Shanghai University of Medicine&Health Sciences Affiliated Zhoupu HospitalShanghai 201318China orthopedic department Guangming Traditional Chinese Medicine Hospital of Pudong New AreaShanghai 201399China

Objective:Peritoneal fibrosis(PF)is the main cause of declining efficiency and ultrafiltration failure of the peritoneum,which restricts the long-term application of peritoneal dialysis(PD).This study aimed to investigate the therapeutic effects and mechanisms of bone marrow mesenchymal stem cells-derived exosomes(BMSC-Exos)on PF in response to ***:Small RNA sequencing analysis of BMSC-Exos was performed by second-generation sequencing.C57BL/6J mice were infused with 4.25%glucose-based peritoneal dialysis fluid(PDF)for 6 consecutive weeks to establish a PF model.A total of 36 mice were randomly divided into 6 groups:control group,1.5%PDF group,2.5%PDF group,4.25%PDF group,BMSC-Exos treatment group,and BMSC-Exos+TP53 treatment *** transcription quantitative polymerase chain reaction(RT-qPCR)was performed to measure the expression level of miR-27a-3p in BMSC-Exos and peritoneum of mice treated with different concentrations of *** and Masson staining were performed to evaluate the extent of *** therapeutic potential of BMSC-Exos for PF was examined through pathological examination,RT-qPCR,Western blotting,and peritoneal function ***-mesenchymal transition(EMT)of HMrSV5 was induced with 4.25%*** were divided into control group,4.25%PDF group,BMSC-Exos treatment group,and BMSC-Exos+TP53 treatment *** Counting Kit-8 assay was used to measure cell viability,and transwell migration assay was used to verify the capacity of BMSC-Exos to inhibit EMT in HMrSV5 ***:Small RNA sequencing analysis showed that miR-27a-3p was highly expressed in BMSC-derived exosomes compared to *** RT-qPCR results showed that the expression of miR-27a-3p was upregulated in BMSC-Exos,but decreased in PD *** found that PF was glucose concentration-dependently enhanced in the peritoneum of the PD *** with the control mice,the PD mice showed high solute transport and decreased ultrafiltration volume as well as an obvious f

关键词： peritoneal fibrosis bone marrow mesenchymal stem cell-derived exosomes miR-27a-3p TP53 epithelialmesenchymal transition