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Neural Regeneration Research 2015年第7期10卷 1139-1146页

作者： Wan-shu Peng Chao Qi Hong Zhang Mei-ling Gao Hong Wang Fei Ren Xia-qing Li department of pathophysiology Shanxi Medical University

Paired immunoglobulin-like receptor B（Pir B） is a functional receptor of myelin-associated inhibitors for axonal regeneration and synaptic plasticity in the central nervous system, and thus suppresses nerve regeneration. The regulatory effect of Pir B on injured nerves has received a lot of attention. To better understand nerve regeneration inability after spinal cord injury, this study aimed to investigate the distribution of Pir B（via immunofluorescence） in the central nervous system and peripheral nervous system 10 days after injury. Immunoreactivity for Pir B increased in the dorsal root ganglia, sciatic nerves, and spinal cord segments. In the dorsal root ganglia and sciatic nerves, Pir B was mainly distributed along neuronal and axonal membranes. Pir B was found to exhibit a diffuse, intricate distribution in the dorsal and ventral regions. Immunoreactivity for Pir B was enhanced in some cortical neurons located in the bilateral precentral gyri. Overall, the findings suggest a pattern of Pir B immunoreactivity in the nervous system after unilateral spinal transection injury, and also indicate that Pir B may suppress repair after injury.

关键词： nerve regeneration paired immunoglobulin-like receptor B myelin inhibitory factor spinal cord injury peripheral nervous system central nervous system cerebral cortex dorsal root ganglion neural regeneration

Portopulmonary hypertension and serum endothelin levels in hospitalized patients with cirrhosis

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Hepatobiliary & Pancreatic Diseases International 2011年第4期10卷 393-398页

作者： Aristotelis Tsiakalos Gregorios Hatzis Ioannis Moyssakis Aggelos Karatzaferis Panayiotis D Ziakas George E Tzelepis department of pathophysiology Medical SchoolNational and Kapodistrian University of AthensAthens 11527Greece department of Cardiology Laikon General HospitalAthensGreece

BACKGROUND:Cirrhosis is associated with several extrahepatic manifestations including portopulmonary hypertension (PPHT).Recent data suggest that endothelins (ETs) are related to the pathophysiology of *** study aimed to measure serum ET levels in hospitalized cirrhotic patients and to determine their association with PPHT and patient ***:Fifty-seven cirrhotic patients [43 males;median age 58 (28-87) years] underwent Doppler *** with systolic pulmonary arterial pressure ≥40 mmHg and pulmonary acceleration time <100 ms were deemed to have ***-1,2,and 3 serum levels were measured with an ELISA ***-cause mortality was recorded over a median period of 24 ***:Nine out of 57 patients (15.8%) had *** various clinical variables,only autoimmune hepatitis was associated with PPHT (OR=11.5;95% CI,1.58-83.4;P=0.01).ET-1 levels [9.1 (1.6-20.7) vs 2.5 (1.4-9.2) pg/mL,P=0.02] and the ET-1/ET-3 ratio [4.73 (0.9-22.4) vs 1.6 (0.3-10.7),P=0.02] were significantly higher in patients with PPHT than in those ***-2 and ET-3 levels did not differ between the two *** was no difference in survival between the two groups,although ET-1 levels were associated with an adverse outcome in Cox regression analysis (HR=1.11;95% CI,1.02-1.22;P=0.02 per unit increase in ET-1).CONCLUSION:Our data suggest that ET-1 and the ET-1/ET-3 ratio are elevated in patients with PPHT and that ET-1 is associated with a poor outcome irrespective of PPHT.

关键词： cirrhosis portopulmonary hypertension endothelin

Inhibition of neurite outgrowth using commercial myelin associated glycoprotein-Fc in neuro-2a cells

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Neural Regeneration Research 2018年第11期13卷 1893-1899页

作者： Fu Liu Mei-Ling Gao Juan Bai Ya-Fang Wang Xia-Qing Li department of pathophysiology Shanxi Medical University Taiyuan Shanxi Province China department of Vascular Surgery People's Hospital of Gansu Province Lanzhou Gansu Province China

Myelin-associated glycoprotein（MAG） inhibits the growth of neurites from nerve cells. Extraction and purification of MAG require complex operations; therefore, we attempted to determine whether commercially available MAG-Fc can replace endogenous MAG for research purposes. Immunofluorescence using specific antibodies against MAG, Nogo receptor（NgR） and paired immunoglobulin-like receptor B（PirB） was used to determine whether MAG-Fc can be endocytosed by neuro-2a cells. In addition, neurite outgrowth of neuro-2a cells treated with different doses of MAG-Fc was evaluated. Enzyme linked immunosorbent assays were used to measure RhoA activity. Western blot assays were conducted to assess Rho-associated protein kinase（ROCK） phosphorylation. Neuro-2a cells expressed NgR and PirB, and MAG-Fc could be endocytosed by binding to NgR and PirB. This activated intracellular signaling pathways to increase RhoA activity and ROCK phosphorylation, ultimately inhibiting neurite outgrowth. These findings not only verify that MAG-Fc can inhibit the growth of neural neurites by activating RhoA signaling pathways, similarly to endogenous MAG, but also clearly demonstrate that commercial MAG-Fc is suitable for experimental studies of neurite outgrowth.

关键词： nerve regeneration myelin growth inhibitors myelin-associated glycoprotein MAG-Fc cell culture receptors for myelin-associatedglycoprotein neuro-2a cell line RhoA/ROCK signaling pathways neurite outgrowth neural regeneration

Role of circular RNAs in gastrointestinal tumors and drug resistance

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World Journal of Clinical Cases 2021年第34期9卷 10400-10417页

作者： Shi-Jun Xi Wen-Qi Cai Qin-Qi Wang Xiao-Chun Peng department of pathophysiology School of Basic MedicineHealth Science CenterYangtze UniversityJingzhou 434023Hubei ProvinceChina

The incidence of gastrointestinal cancers has increased significantly over the past decade and gastrointestinal malignancies now rank among the leading causes of mortality *** newer therapeutic strategies such as targeted therapies have greatly improved patient outcomes,their clinical success is limited by drug resistance,treatment failure and recurrence of metastatic ***,there is an urgent need for further research identifying accurate and reliable biomarkers for precise treatment *** RNAs(circRNAs)exhibit a covalently closed structure,high stability and biological conservation,and their expression is associated with the occurrence and development of gastrointestinal ***,circRNAs may significantly influence drug resistance of gastrointestinal *** this article,we review the role of circRNAs in the occurrence and development of gastrointestinal cancer,their association with drug resistance,and potential application for early diagnosis,treatment and prognosis in gastrointestinal ***,we summarize characteristics of circRNA,including mechanism of formation and biological effects via mRNA sponging,chromatin replication,gene regulation,translational modification,signal transduction,and damage ***,we discuss whether circRNA-related noninvasive testing may be clinically provided in the *** review provides new insights for the future development of diagnostics and therapeutics based on circRNAs in gastrointestinal tumors.

关键词： Gastrointestinal cancer Circular RNA Drug resistance Genomics Targeted therapy Molecular mechanics

The Anti-tumor Immunity of Dendritic Cells Modified by IFN γ Gene on Mice Bearing Ascite Hepatoma Cell H22

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生物医学工程学杂志 2005年第S1期22卷 109-110页

作者： Zi-You CUI Hong-Yan YANG You-Tian HUANG Zhi-min ZHENGMing-Yao ZHAO Zi-Ming DONG(department of pathophysiology, Basic Medical College, Zhengzhou University, Zhengzhou 450052,China) department of pathophysiology Basic Medical College Zhengzhou University Zhengzhou 450052 China

1 IntroductionDendritc cell (DC)-based cancer vaccines have shown to been effective both in clinical trials and in animal tumor models. Some clinical trials have been on the phase Ⅲ , but some problems are challenging now. The functions of DC from patient with malignant tumor were depressed by tumor-secreting cytokines such as IL-10. it is critical to find out some methods to improve DC differentiation maturation for priming naive T cells and initiating the specific anti-tumor immunity effectively. IFNγ is a pluripotent cytokine that can exert more the expressions of different molecules in various cells. Now, some data have shown that DCs can produce IFNγ and IFNγ can promote the maturation of DCs, which plays very important roles in promoting protective immune response as the same as IFNγ produced in NK and NKT cells. In our research,we transfected IFNγ gene into DCs in order to investigate the effect of IFNγ on DCs and monitor the anti-tumor response of the tumor bearing mice after vaccination by IFNγ-modified DCs.

关键词： The Anti-tumor Immunity of Dendritic Cells Modified by IFN Gene on Mice Bearing Ascite Hepatoma Cell H22 Cell

Family with sequence similarity 134 member B-mediated reticulophagy ameliorates hepatocyte apoptosis induced by dithiothreitol

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World Journal of Gastroenterology 2022年第23期28卷 2569-2581页

作者： Yi-Xin Guo Bing Han Ting Yang Yu-Si Chen Yi Yang Jia-Yao Li Qin Yang Ru-Jia Xie department of pathophysiology Guizhou Provincial Key Laboratory of Pathogenesis and Drug Research on Common Chronic DiseasesGuizhou Medical UniversityGuiyang 550025Guizhou ProvinceChina department of pathophysiology College of Basic Medical SciencesGuizhou Medical UniversityGuiyang 550025Guizhou ProvinceChina

BACKGROUND Endoplasmic reticulum(ER)stress-related hepatocyte apoptosis is responsible for multiple hepatic *** studies have revealed that endoplasmic reticulophagy(ER-phagy)promotes the selective clearance of damaged ER fragments during ER stress,playing a crucial role in maintaining ER homeostasis and inhibiting *** with sequence similarity 134 member B(FAM134B)is a receptor involved in ER-phagy that can form a complex with calnexin(CNX)and microtubule-associated protein 1 light chain 3(LC3).The complex can mediate the selective isolation of ER fragments to attenuate hepatocyte ***,the precise regulatory mechanisms remain *** To elucidate the effect of FAM134B-mediated ER-phagy on ER stress-induced apoptosis in buffalo rat liver 3A(BRL-3A)rat hepatocytes and the potential regulatory *** ER stress-related hepatocyte apoptosis was induced using dithiothreitol(DTT).Proteins related to ER stress and autophagy were measured with western *** complex interactions with FAM134B were isolated by ***-phagy was evaluated in immunofluorescence *** cycle distribution and apoptosis were measured by flow *** Ca^(2+) levels were evaluated by the co-localization of intracellular Ca^(2+)-tracker and *** small interfering RNA against FAM134B was used to knockdown FAM134B in BRL-3A *** ER stress-related and autophagy-related proteins in BRL-3A cells were elevated by both short and long-term DTT ***,co-immunoprecipitation confirmed an interaction between FAM134B,CNX,FAM134B,and LC3 in BRL-3A *** assays revealed that autolysosomes significantly decreased following short-term DTT treatment,but increased after long-term *** Ca2+levels and apoptotic rates were dramatically elevated,and more cells were arrested in the G1 stage after short-term DTT treatment;however,these decreased

关键词： Hepatocytes Reticulophagy Family with sequence similarity 134 member B Apoptosis Endoplasmic reticulum stress Endoplasmic reticulum homeostasis

PROTECTION AGAINST LEPTOSPIROSIS BY IMMU NIZATION WITH PLASMID DNA ENCODING 33 kDa ENDOFLAGELLIN OF *** SEROVAR LAI

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Chinese Medical Sciences Journal 2000年第1期15卷 14-19页

作者：戴保民游自立陈庄阎和平方之茂 Research Unit of Leptospirosis Department of Pathophysiology West China University of Medical Sciences Chendu 610044

To evaluate how the efficacy of DNA inocutation affects the ability to raise protective immunity against Leptospira. [WT5”BX]Methods.[WT5”BZ] A pair of oligonucleotide primers were designed to amplify the endoflagellar gene of L. interrogans sensu stricto serovar lai. An approximately 840bp fragment was generated with PCR and inserted into VR1012, a plasmid DNA expression vector, after the fragment and VR1012 were digested respectively with EcoRV and Sal I. A recombinant plasmid designated as VR1012+flaB2 was obtained. The vector, VR1012 consits of a pUC18 backbone with the cytomegalovirus(CMV) IE1 enhancer, promoter, and intron A, transcription regulatory elements and the BGH polyadenylation sequences driving the expressing of leptospiral endoflagellar gene of L. interrogans sensu stricto serovar lai. Plasmid encoding leptospiral endoflagellin gene was injected into quadriceps of NZW rabbits. [WT5”BX]Results.[WT5”BZ]This resulted in the generation of specific leptospiral antibody with high ELISA titer (1:32768) in the rabbits. Immuno/protection was performed in guinea pigs without adjuvant. The group“VR1012+flaB2” showed higher survival rate(90%,9/10 animals),compared with the group “VR1012 lack flaB2” and the group “normal saline”. [WT5”BX]Conclusion.[WT5”BZ]The technique of DNA vaccine has potential advantages over certain other vaccine preparation technologies. However whether DNA vaccine will be useful for vaccine development remains to be tested.

关键词： leptospira endoflagellin DNA vaccine

Characterization of MR-1,a Novel Myofibrillogenesis Regulator in Human Muscle

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Acta Biochimica et Biophysica Sinica 2004年第6期36卷 412-418页

作者： Tian-Bo LI, Xiu-Hua LIU1, Shuang FENG, Yang HU, Wei-Xi YANG, Yue HAN1, Yi-Guang WANG*, and Li-Min GONG2*Institute of Medicinal Biotechnology, CAMS and PUMC, Beijing 100050, China 1department of pathophysiology, PLA General Hospital, Beijing 100853, China 2department of Cardiology, the University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA Institute of Medicinal Biotechnology CAMS and PUMCBeijing 100050 China department of pathophysiology PLA General HospitalBeijing 100853 China department of Cardiology the University of Texas MD Anderson Cancer CenterHouston TX 77030 USA

The actin-myosin contractile apparatus consists of several thick filament and thin filament proteins. Specific regulatory mechanisms are involved in this highly ordered process. In this paper, we reported the identification and characterization of a novel myofibrillogenesis regulator, MR-1. The MR-1 gene was cloned from human skeletal muscle cDNA library by using a strategy that involves EST data base searching, PCR and RACE. The MR-1 gene is located on human chromosome 2q35 and encodes a 142 aa protein. Northern blot revealed that the mRNA level of MR-1 was highest in the skeletal muscle and certain level of MR-1 expression was also observed in heart, liver and kidney. Immunohistochemical assay confirmed that the MR-1 protein existed in human myocardial myofibrils. It was found by yeast two-hybrid screening and confirmed by in vitro binding assay that MR-1 could interact with sarcomeric proteins, such as myosin regulatory light chain, myomesin 1 and β-enolase. These studies suggested that MR-1 might play a regulatory role in the muscle cell and it was worth investigating further.

关键词： MR-1 gene cloning heart muscle yeast two-hybrid

Nucleolin Mediates LPS-induced Expression of Inflammatory Mediators and Activation of Signaling Pathways

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Current Medical Science 2020年第4期40卷 646-653页

作者： Li FANG Kang-kai WANG Qing HUANG Feng CHENG Fang HUANG Wei-wei LIU department of Cardiology the First Hospital of ChangshaChangsha 410008China department of pathophysiology Xiangya School of MedicineCentral South UniversityChangsha 410008China

Summary:In this study,we investigated the effects of nucleolin on lipopolysaccharide(LPS)-induced activation of MAPK and NF-KappaB(NF-kB)signaling pathways and secretion of TNF-a,IL-1βand HMGB1 in THP-1 *** assay and Western blotting were used to identify the nucleolin expression in cell membrane,cytoplasm and nucleus of THP-1 *** of nucleolin was induced by neutralizing antibody against ***-1 monocytes were pretreated with anti-nucleolin antibody for 1 h prior to LPS *** irrelevant IgG group was used as *** of inflammatory mediators(TNF-a,IL-1β and HMGB1)and activation of MAPK and NF-kB/I-kB signaling pathways were examined to assess the effects of nucleolin on LPS-mediated inflammatory *** existed in cell membrane,cytoplasm and nucleus of THP-1 *** of anti-nucleolin antibody significantly inhibited the LPS-induced secretion of TNF-a,IL-1β and HMGB1.P38,JNK,ERK and NF-κB subunit p65 inhibitors could significantly inhibit the secretion of IL-1β,TNF-a and HMGB1 induced by ***,the phosphorylation of p38,JNK,ERK and p65(or nuclear translocation of p65)was significantly increased after LPS *** contrast,pretreatment of anti-nucleolin antibody could significantly inhibit the LPS-induced phosphorylation of p38,JNK,ERK and p65(or nuclear translocation of p65).However,the irrelevant IgG,as a negative control,had no effect on LPS-induced secretion of TNF-a and IL-Iβ and phosphorylation of p38,JNK,ERK and p65(or nuclear translocation of p65).We demonstrated that nucleolin mediated the LPS-induced activation of MAPK and NF-κB signaling pathways,and regulated the secretion of inflammatory mediators(TNF-a,IL-1β and HMGB1).

关键词： nucleolin THP-1 monocytes lipopolysaccharide MAPK NF-κB signaling pathway inflammatory mediators

Role of serotonin in modulating the development and function of adult-born neurons in the olfactory bulb

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Neural Regeneration Research 2022年第6期17卷 1253-1254页

作者： Natalie Fomin-Thunemann Olga Garaschuk department of Biomedical Engineering Boston UniversityBostonMAUSA Institute of Physiology Department NeurophysiologyEberhard Karls University of TübingenTübingenGermany

The neuromodulatory transmitter serotonin(5-hydroxytryptamine,5-HT)is synthesized by neurons located in the brainstem,which project more or less densely to the entire central nervous system(Charnay and Leger,2010).Serotonin regulates a variety of physiological functions,including food intake,reward,reproduction,sleep-wake cycle,memory,cognition,emotion,and mood(Charnay and Leger,2010).

关键词： serotonin intake modulator