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Experimental Autoimmune Encephalomyelitis Inhibited by Huangqi Guizhi Wuwu Decoction via Th2 Cytokine Enhancement

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World Journal of Traditional Chinese Medicine 2021年第4期7卷 467-476页

作者： Yong Peng Fei-Zhou Zhu Xiang Deng Jian-Xiong Zhou Shuai Gao Zhi-Xing Chen Shan-Shan Yang Lu Gan Zhuo-Lin Li Qian-Qian Liu department of Neurology Affiliated First Hospital of Hunan Traditional Chinese Medical CollegeZhuzhou department of Biochemistry and molecular biology School of Life SciencesCentral South UniversityChangshaHunanChina

Background:Huangqi Guizhi Wuwu decoction(HQGZWW)exhibits good effects when administered to treat multiple sclerosis(MS)and its animal model,experimental autoimmune encephalomyelitis(EAE).Understanding the precise mechanism of this decoction is thus *** on the findings of our previous study,the aim of the present study was to understand the role of antigen-specific CD8^(+)T-cells on the pathogene sis of MS/EAE when HQGZWW is administered as ***:Myelin oligodendrocyte glycoprotein(MOG);-induced mice were administered distilled water,prednisone,and high dose or low dose *** purified CD4^(+)and CD8^(+)T-cells were stimulated with the MOG;peptide,proliferation and cytokine secretion assays were *** establish the adoptive transfer EAE model,naive mice were injected with MOG;-CD8^(+)or CD4^(+)***:Significant improvements in EAE score and pathology were observed in the high dose HQGZWW and prednisone *** to the low dose HQGZWW and distilled water groups,lower antigen-specific re sponses,lower levels of interferon-gamma,and higher levels of interleukin(IL)-4 and IL-10 from CD8^(+)and CD4^(+)T cells were observed in the high dose HQGZWW and prednisone ***,the EAE score was observed to be similar between the high dose HQGZWW group and prednisone group;however,this finding was not observed in the low dose HQGZWW ***:Our findings suggest that high dose HQGZWW has similar effects on cell proliferation,cytokine secretion,and EAE score to prednisone,while low dose HQGZWW does not have such *** protective role of HQGZWW against EAE might thus depend on the Th2 cytokine secretion profile induced by either MOG;specific CD8^(+)or CD4^(+)T-cells.

关键词： CD8-Positive T-lymphocytes experimental autoimmune encephalomyelitis multiple sclerosis myelin-oligodendrocyte glycoprotein Th2 cytokine traditional Chinese medicine

Exon-centric regulation of pyruvate kinase M alternative splicing via mutually exclusive exons

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分子细胞生物学报:英文版 2012年第2期4卷 79-87页

作者： Zhenxun Wang Deblina Chatterjee Hyun Yong Jeon Martin Akerman Matthew G.Vander Heiden Lewis C.Cantley Adrian R.Krainer Cold Spring Harbor Laboratory PO Box 100Cold Spring HarborNY 11724USA Watson School of Biological Sciences Cold Spring HarborNY 11724USA Graduate Program in molecular and Cellular biology Stony Brook UniversityStony BrookNY 11794USA Koch Institute for Integrative Cancer Research at MIT CambridgeMA 02139USA Division of Signal Transduction Beth Israel Deaconess Medical Center and Department of Systems BiologyHarvard Medical SchoolBostonMA 02115USA

Alternative splicing of the pyruvate kinase M gene(PK-M)can generate the M2 isoform and promote aerobic glycolysis and tumor ***,the cancer-specific alternative splicing regulation of PK-M is not completely ***,we demonstrate that PK-M is regulated by reciprocal effects on the mutually exclusive exons 9 and 10,such that exon 9 is repressed and exon 10 is activated in cancer ***,exonic,rather than intronic,cis-elements are key determinants of PK-M splicing isoform *** a systematic sub-exonic duplication approach,we identify a potent exonic splicing enhancer in exon 10,which differs from its homologous counterpart in exon 9 by only two *** identify SRSF3 as one of the cognate factors,and show that this serine/arginine-rich protein activates exon 10 and mediates changes in glucose *** findings provide mechanistic insights into the complex regulation of alternative splicing of a key regulator of the Warburg effect,and also have implications for other genes with a similar pattern of alternative splicing.

关键词： alternative splicing cancer metabolism pyruvate kinase SRSF3

Capn3 depletion causes Chk1 and Wee1 accumulation and disrupts synchronization of cell cycle reentry during liver regeneration after partial hepatectomy

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Cell Regeneration 2020年第1期9卷 74-91页

作者： Feng Chen Delai Huang Hui Shi Ce Gao Yingchun Wang Jinrong Peng MOE Key Laboratory for molecular Animal Nutrition College of Animal SciencesZhejiang UniversityHangzhou 310058China Present address:department of biology University of VirginiaCharlottesvilleVAUSA Present address:department of Pediatric Oncology Dana-Farber Cancer InstituteHarvard Medical SchoolBostonMAUSA State Key Laboratory of molecular Developmental biology Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China

Recovery of liver mass to a healthy liver donor by compensatory regeneration after partial hepatectomy(PH)is a prerequisite for liver *** cell cycle reentry of the existing hepatocytes after PH is seemingly a hallmark of liver compensatory *** the molecular control of the PH-triggered cell cycle reentry has been extensively studied,little is known about how the synchronization is achieved after *** nucleolus-localized protein cleavage complex formed by the nucleolar protein Digestive-organ expansion factor(Def)and cysteine proteinase Calpain 3(Capn3)has been implicated to control wounding healing during liver regeneration through selectively cleaving the tumor suppressor p53 in the ***,whether the Def-Capn3 complex participates in regulating the synchronization of cell cycle reentry after PH is *** this report,we generated a zebrafish capn3b null mutant(capn3b^(Δ19Δ14)).The homozygous mutant was viable and fertile,but suffered from a delayed liver regeneration after *** liver regeneration in capn3b^(Δ19Δ14)was due to disruption of synchronized cell proliferation after *** spectrometry(MS)analysis of nuclear proteins revealed that a number of negative regulators of cell cycle are accumulated in the capn3b^(Δ19Δ14)liver after ***,we demonstrated that Check-point kinase 1(Chk1)and Wee1,two key negative regulators of G2 to M transition,are substrates of *** also demonstrated that Chk1 and Wee1 were abnormally accumulated in the nucleoli of amputated capn3bΔ19Δ14 *** conclusion,our findings suggest that the nucleolar-localized Def-Capn3 complex acts as a novel regulatory pathway for the synchronization of cell cycle reentry,at least partially,through inactivating Chk1 and Wee1 during liver regeneration after PH.

关键词： Def Capn3 Chk1 Wee1 Nucleolus Cell cycle Liver regeneration Partial hepatectomy Zebrafish

Lipid droplet dynamics in alcoholic fatty liver disease

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Liver Research 2019年第3期3卷 185-190页

作者： Ryan J.Schulze Wen-Xing Ding department of Biochemistry and molecular biology and the Division of Gastroenterology and Hepatology Mayo ClinicRochesterMNUSA department of Pharmacology Toxicologyand TherapeuticsThe University of Kansas Medical CenterKansas CityKSUSA

The rising incidence of alcohol-related liver disease(ALD)demands making urgent progress in understanding the fundamental molecular basis of alcohol-related hepatocellular *** of the key early events accompanying chronic alcohol usage is the accumulation of lipid droplets(LDs)in the hepatocellular *** are far from inert sites of neutral lipid storage;rather,they represent key organelles that play vital roles in the metabolic state of the *** this review,we will examine the biology of these structures and outline recent efforts being made to understand the effects of alcohol exposure on the biogenesis,catabolism,and motility of LDs and how their dynamic nature is perturbed in the context of ALD.

关键词： Lipid droplet(LD) Alcohol Autophagy Lipolysis Hepatocyte Steatosis

Receptor-Like Kinases:Key Regulators of Plant Development and Defense

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Journal of Integrative Plant biology 2013年第12期55卷 1184-1187页

作者： Jia Li Frans E.Tax Special Issue Editor Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations School of Life SciencesLanzhou University Special Issue Editor department of molecular and Cellular biology University of Arizona

Plants are multi-cellular organisms that live in diverse and fluctuating environments. Cell-cell and cell-environment com- munication are therefore critical to plant growth and develop- ment. In animals, transmembrane receptor protein tyrosine kinases play significant roles in cell-cell signaling. There was a great deal of surprise in the plant community, however, when the first receptor-like protein kinase （RLK） was isolated from maize by John C.

关键词： Receptor-Like Kinases

Blue-light-induced rapid chloroplast de-anchoring in Vallisneria epidermal cells

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Journal of Integrative Plant biology 2015年第1期57卷 93-105页

作者： Yuuki Sakai Shin-Ichiro Inoue Akiko Harada Ken-Ichiro Shimazaki Shingo Takagi department of Biological Sciences Graduate School of Science Osaka University Division of Biological Science Graduate School of Science Nagoya University department of biology Osaka Medical College department of biology Faculty of Science Kyushu University

In the outer periclinal cytoplasm of leaf epidermal cells of an aquatic angiosperm Vallisneria, blue light induces ＂chloroplast de-anchoring＂, a rapid decline in the resistance of chloroplasts against centrifugal force. Chloroplast de- anchoring is known induced within lmin of irradiation with high-fluence-rate blue light specifically, preceding the com- mencement of chloroplasts migration toward the anticlinal cytoplasm. However, its regulatory mechanism has remained elusive, although pharmacological analysis suggested that a calcium release from intracellular calcium stores is necessary for the response, in search of the responsible photoreceptors, immunoblotting analysis using antibodies against phototropins demonstrated that cross-reactive polypeptides of 120-kDa exist in the plasma-membrane fraction prepared from the leaves. In vitro phosphorylation analysis revealed that 120-kDa polypeptides were phosphorylated by exposure to blue light in a fluence-dependent manner. The blue-light-induced phosphorylation activity was sensitive to a Ser/Thr kinase inhibitor, staurosporine, and unusually was retained at a high level for a long time in darkness. Furthermore, phototropin gene homologs （Vallisneria PHOTOTROPINI and PHOTOTROPIN2） expressed in leaves were isolated. We propose that calcium- regulated chloroplast de-anchoring, possibly mediated by phototropins, is an initial process of the blue-light-induced avoidance response of chloroplasts in Vallisneria.

关键词： Avoidance response of chloroplasts blue-light response chloroplast de-anchoring cytoplasmic calcium phototropin

Double plasma molecular adsorption system for Stevens–Johnson syndrome/toxic epidermal necrolysis:A case report

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World Journal of Clinical Cases 2024年第7期12卷 1371-1377页

作者： You-Wen Tan Li-Ping Liu Kai Zhang department of Hepatology The Third Hospital of Zhenjiang Affiliated Jiangsu UniversityZhenjiang 212003Jiangsu ProvinceChina

BACKGROUND Stevens–Johnson syndrome and toxic epidermal necrolysis(SJS/TEN)are very serious skin allergies,with an etiology related to infections and *** the coronavirus disease 2019(COVID-19)pandemic,severe acute respiratory syndrome coronavirus-2 has also been considered to cause SJS/*** SUMMARY We report the case of a woman in her thirties who took acetaminophen after contracting *** 3 d of fever relief,she experienced high fever and presented with SJS/TEN symptoms,accompanied by intrahepatic *** days of corticosteroid treatment did not alleviate the skin damage;therefore,double plasma molecular adsorption system(DPMAS)therapy was initiated,with treatment intervals of 48 *** skin symptoms improved gradually and were resolved after seven DPMAS *** DPMAS therapy is beneficial for abrogating SJS/TEN because plasma adsorption and perfusion techniques reduce the inflammatory mediators(e.g.,tumor necrosis factor-alpha and interleukin-10 and-12)speculated to be involved in the pathology of the skin conditions.

关键词： Stevens-Johnson Toxic epidermal necrolysis COVID-19 Double plasma molecular adsorption system SARS-CoV-2 Case report

The expression of proteins involved in digestion and detoxification are regulated in Helicoverpa armigera to cope up with chlorpyrifos insecticide

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Insect Science 2016年第1期23卷 68-77页

作者： Vishal V. Dawkar Yojana R. Chikate Tushar H. More Vidya S. Gupta Ashok P. Giri Plant molecular biology Unit Division of Biochemical Sciences CSIR-National Chemical Laboratory Dr. Homi Bhabha Road Pun e 411008(MS) India

Helicoverpa armigera is a key pest in many vital crops, which is mainly controlled by chemical strategies, To manage this pest is becoming challenging due to its ability and evolution of resistance against insecticides. Further, its subsequent spread on nonhost plant is remarkable in recent times. Hence, decoding resistance mechanism against phytochemicals and synthetic insecticides is a major challenge. The present work describes that the digestion, defense and immunity related enzymes are associated with chlorpyrifos resistance in 11. armigera. Proteomic analysis of/-/, armigera gut tissue upon feeding on chlorpyrifos containing diet （CH） and artificial diet （AD） using nano-liquid chromatography-mass spectrometry identified upregulated 23-proteins in CH fed larvae. Database searches combined with gene ontology analysis revealed that the identified gut proteins engrossed in digestion, proteins crucial for immunity, adaptive responses to stress, and detoxification. Biochemical and quantitative real-time polymerase chain reaction anal- ysis of candidate proteins indicated that insects were struggling to get nutrients and energy in presence of CH, while at the same time endeavoring to metabolize chlorpyrifos. More- over, we proposed a potential processing pathway of chlorpyrifos in H. armigera gut by examining the metabolites using gas chromatography-mass spectrometry. 1f. armigera exhibit a range of intriguing behavioral, morphological adaptations and resistance to in- secticides by regulating expression of proteins involved in digestion and detoxification mechanisms to cope up with chlorpyrifos. In these contexts, as gut is a rich repository of biological information; profound analysis of gut tissues can give clues of detoxification and resistance mechanism in insects.

关键词： chlorpyrifos Helicoverpa armigera insecticide resistance proteomics real-time PCR

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古基因组揭示河西走廊两千年以来的人群历史

Science Bulletin 2024年第5期69卷 606-611页

作者：熊建雪王睿陈国科杨谊时杜盼新蒙海亮马敏敏安达陶乐王辉金力王传超文少卿 Institute of Archaeological Science Fudan UniversityShanghai 200433China State Key Laboratory of Cellular Stress biology School of Life SciencesXiamen UniversityXiamen 361102China Institute of Cultural Relics and Archaeology in Gansu Province Lanzhou 730000China State Key Laboratory of Genetic Engineering Collaborative Innovation Center for Genetics and DevelopmentSchool of Life Sciencesand Human Phenome InstituteFudan UniversityShanghai 201203China Ministry of Education Key Laboratory of Contemporary Anthropology Department of Anthropology and Human GeneticsSchool of Life SciencesFudan UniversityShanghai 200438China Key Laboratory of Western China’s Environmental systems(Ministry of Education) College of Earth and Environmental ScienceLanzhou UniversityLanzhou 730000China Center for the Belt and Road Archaeology and Ancient Civilizations Fudan UniversityShanghai 200433China department of Anthropology and Ethnology Institute of AnthropologySchool of Sociology and AnthropologyFujian Provincial Key Laboratory of Philosophy and Social Sciences in BioanthropologyXiamen UniversityXiamen 361005China State Key Laboratory of Marine Environmental Science Xiamen UniversityXiamen 361102China Institute of Artificial Intelligence Xiamen UniversityXiamen 361005China MOE Laboratory for National Development and Intelligent Governance Fudan UniversityShanghai 200433China

The Hexi Corridor in northwest China has for millennia served as a crossroads for interactions between East Asia,Central Asia and lands further west,while also connecting the Chinese heartland and the northern Mongolian Steppe and Tibetan Plateau(TP)to the south(Fig.1a).Previous studies have revealed the details of this cross-continental material and cultural transmission as early as the Bronze Age *** and technologies exchanged along the ancient Silk Road included East Asian millets and painted pottery,West Asian wheat and barley,bronze metal-lurgy,domesticated sheep,horses,and cattle[1].To date.

关键词： connecting details northwest